Rroxscaffold_5G00342970

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0014710
Physical Location & Seq
Forward (+)
12120848 .. 12141395
20548 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00342970.1

Sequence Viewer

Length: 1158 bp
ATGGCTGGCAATGTTTGCTATGGCCGAGACAGCGGTGTTGGTGCTTTGGCGGGGTGGTCACCAGGAGGTGAAAACCCTTTTCAACCACGTTTTCTGATTGCGCTTTGCAATCGAGAAATGGCAAGTAGAGGAGGAAGAAAAGGCTTCAACATCAGAGCTTCTTCCAGTGCAGCTGAAGTGGGCAAGTCCGATGATCTCTTGAATGAGATTCTTGTTCGCCTCCCAGCCAAACCAATTCTCAAGTTCAAATTAGTTTCAAAACAATGGTACTCCATAATCTCTACCCAAAAATTCAGTCACTTATGCCAAAAGAAACCCGAAGCCTCACTTGGGGGTTTTGTTGCATTCTCTACTAAATTTCCAACCATACCCACCTACTCTCTTCCTTTCTCTGGTGGCTATCAATATGATGAGTGCCATTTGATCAAAATTGATATTTACTCATCTGAGACTGAGTCTTGGAGACCCTCCAAGAAGGGGATTTATATTTTGCAATACAATATAGGGTTTCACATCGTAGTTTACTGGAAGGATGCAATTTACTGGTACAATCAGAAGAATCAGTCTTTGGCACATTTCAATTTCGATGATGACAGCGTGGAGAATTTGCCTAAGCCACAAGTTTCTGGAACCAGGATTTATTACTATTTTAGCGAGTCAGGTGGCCATTTGCATCTTGCTGGAGCTTGTAGACCTTGGACTACATTGTTCACTGTCTTCGAAATGAAGGAAGATAGGTCTGGGTGGTTTGTGAAGCATAATGGTGATTTTAGTGCTTTAGCTGCTGCATTTCCGAAGATGGTTACTAATAAACCAAATAGGCCTTATGTGTTTTCTCTGGTGAACCTGTTAAGAGGTGAAAAGGAAGAGGAGTTGATTATTGTGTTAACAATTCTCGAAGGTGTGATTGGGTATAATGTCCAGGATAAGCCTTTGAGAAGCTTAGAGCCACAAAAGGATGTGGCCTCTAAGGATCTTTACCAAGTAAAACAAGAGATGAAGAAAAATGAAAGGAAAGGAAGAAGTATGCAAAGTGAGAAGAGTATTTCGGTCAACAGTACCCTCGATGGGTCATTTGGTCGGCGGTATCCTCCGGATGGCATGAGACGATTAGTTGGCGTACCCTCTAACTATGTTACCCAAGGGCAAAATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

385

Amino Acids

43.49

Weight (kDa)

9.22

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 64 - 98 1.1e-07 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014710)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06290 FvH4_4g06330
malus_domestica MD13G1235000.v1.1
prunus_persica Prupe.1G066100_v2.0.a1 Prupe.1G066200_v2.0.a1
pyrus_communis pycom13g20750
rosa_chinensis RchiOBHm_Chr4g0398011
rosa_laevigata RLG00000014125
rosa_roxburghii Rroxscaffold_5G00342970
rosa_rugosa Rorug04G0005500 Rorug06G0022700
rosa_samantha Rh4AG082200 Rh4BG079700 Rh4DG075100 Rh6BG144300 Rh6DG128700
rosa_wichuraiana Rw4G006860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 691
AccIII TCCGGA 1 cut(s) 1093
AciI CCGC 3 cut(s) 33, 50, 1084
AclWI GGATC 1 cut(s) 981
AcoI YGGCCR 2 cut(s) 22, 664
AcsI RAATTY 3 cut(s) 290, 356, 604
AcuI CTGAAG 1 cut(s) 195
AfaI GTAC 4 cut(s) 269, 548, 1060, 1122
AfiI CCNNNNNNNGG 5 cut(s) 330, 392, 477, 1068, 1097
AgsI TTSAA 6 cut(s) 83, 148, 202, 247, 258, 580
AjnI CCWGG 3 cut(s) 61, 632, 921
AjuI GAANNNNNNNTTGG 6 cut(s) 312, 344, 551, 583, 891, 923
AluBI AGCT 5 cut(s) 158, 173, 686, 782, 942
AluI AGCT 5 cut(s) 158, 173, 686, 782, 942
Alw26I GTCTC 4 cut(s) 21, 443, 457, 1099
AlwI GGATC 1 cut(s) 981
Aor13HI TCCGGA 1 cut(s) 1093
AoxI GGCC 4 cut(s) 22, 664, 821, 963
ApeKI GCWGC 3 cut(s) 170, 782, 785
ApoI RAATTY 3 cut(s) 290, 356, 604
ArsI GACNNNNNNTTYG 2 cut(s) 280, 312
AspLEI GCGC 1 cut(s) 103
AsuHPI GGTGA 5 cut(s) 51, 80, 776, 853, 869
AsuII TTCGAA 1 cut(s) 720
BalI TGGCCA 1 cut(s) 666
BbsI GAAGAC 1 cut(s) 709
BbvI GCAGC 3 cut(s) 182, 769, 772
BccI CCATC 3 cut(s) 793, 1061, 1091
BciT130I CCWGG 3 cut(s) 63, 634, 923
BciVI GTATCC 1 cut(s) 1098
BclI TGATCA 1 cut(s) 423
BcoDI GTCTC 4 cut(s) 21, 443, 457, 1099
BfuI GTATCC 1 cut(s) 1098
BisI GCNGC 3 cut(s) 171, 783, 786
BlsI GCNGC 3 cut(s) 172, 784, 787
Bme1390I CCNGG 3 cut(s) 63, 634, 923
BmiI GGNNCC 1 cut(s) 631
BmrFI CCNGG 3 cut(s) 63, 634, 923
BmsI GCATC 2 cut(s) 523, 682
BpiI GAAGAC 1 cut(s) 709
BpmI CTGGAG 1 cut(s) 702
Bpu10I CCTNAGC 1 cut(s) 612
Bpu14I TTCGAA 1 cut(s) 720
BpuEI CTTGAG 1 cut(s) 224
BsaI GGTCTC 1 cut(s) 457
BsaJI CCNNGG 2 cut(s) 695, 1141
BsaWI WCCGGW 1 cut(s) 1093
Bsc4I CCNNNNNNNGG 5 cut(s) 330, 392, 477, 1068, 1097
Bse1I ACTGG 3 cut(s) 165, 530, 548
Bse3DI GCAATG 1 cut(s) 16
BseAI TCCGGA 1 cut(s) 1093
BseBI CCWGG 3 cut(s) 63, 634, 923
BseDI CCNNGG 2 cut(s) 695, 1141
BseGI GGATG 3 cut(s) 538, 964, 1102
BseLI CCNNNNNNNGG 5 cut(s) 330, 392, 477, 1068, 1097
BseMI GCAATG 1 cut(s) 16
BseMII CTCAG 2 cut(s) 438, 444
BseNI ACTGG 3 cut(s) 165, 530, 548
BseRI GAGGAG 2 cut(s) 144, 884
BseXI GCAGC 3 cut(s) 182, 769, 772
BseYI CCCAGC 1 cut(s) 223
BsgI GTGCAG 1 cut(s) 189
BshFI GGCC 4 cut(s) 24, 666, 823, 965
BsiSI CCGG 1 cut(s) 1094
BslI CCNNNNNNNGG 5 cut(s) 330, 392, 477, 1068, 1097
BsmAI GTCTC 4 cut(s) 21, 443, 457, 1099
BsmBI CGTCTC 1 cut(s) 1099
BsmI GAATGC 1 cut(s) 344
BsnI GGCC 4 cut(s) 24, 666, 823, 965
Bso31I GGTCTC 1 cut(s) 457
Bsp119I TTCGAA 1 cut(s) 720
Bsp13I TCCGGA 1 cut(s) 1093
Bsp143I GATC 3 cut(s) 193, 423, 973
BspACI CCGC 3 cut(s) 33, 50, 1084
BspANI GGCC 4 cut(s) 24, 666, 823, 965
BspCNI CTCAG 2 cut(s) 439, 445
BspEI TCCGGA 1 cut(s) 1093
BspLI GGNNCC 1 cut(s) 631
BspPI GGATC 1 cut(s) 981
BspT104I TTCGAA 1 cut(s) 720
BspTNI GGTCTC 1 cut(s) 457
BsrDI GCAATG 1 cut(s) 16
BsrI ACTGG 3 cut(s) 165, 530, 548
BssECI CCNNGG 2 cut(s) 695, 1141
BssMI GATC 3 cut(s) 193, 423, 973
BssT1I CCWWGG 2 cut(s) 695, 1141
Bst2UI CCWGG 3 cut(s) 63, 634, 923
Bst4CI ACNGT 2 cut(s) 715, 1058
Bst6I CTCTTC 3 cut(s) 387, 861, 1034
BstAPI GCANNNNNTGC 1 cut(s) 15
BstBI TTCGAA 1 cut(s) 720
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 5 cut(s) 447, 453, 612, 943, 969
BstEII GGTNACC 1 cut(s) 57
BstF5I GGATG 3 cut(s) 538, 964, 1102
BstHHI GCGC 1 cut(s) 103
BstKTI GATC 3 cut(s) 196, 426, 976
BstMAI GTCTC 4 cut(s) 21, 443, 457, 1099
BstMBI GATC 3 cut(s) 193, 423, 973
BstMWI GCNNNNNNNGC 3 cut(s) 15, 30, 782
BstNI CCWGG 3 cut(s) 63, 634, 923
BstPI GGTNACC 1 cut(s) 57
BstSCI CCNGG 3 cut(s) 61, 632, 921
BstV1I GCAGC 3 cut(s) 182, 769, 772
BstV2I GAAGAC 1 cut(s) 709
BstX2I RGATCY 1 cut(s) 973
BstYI RGATCY 1 cut(s) 973
BsuI GTATCC 1 cut(s) 1098
BsuRI GGCC 4 cut(s) 24, 666, 823, 965
BtsCI GGATG 3 cut(s) 538, 964, 1102
BtsIMutI CAGTG 2 cut(s) 172, 711
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 1 cut(s) 103
Csp6I GTAC 4 cut(s) 268, 547, 1059, 1121
CviAII CATG 1 cut(s) 1102
CviQI GTAC 4 cut(s) 268, 547, 1059, 1121
DdeI CTNAG 5 cut(s) 447, 453, 612, 943, 969
DpnI GATC 3 cut(s) 195, 425, 975
DpnII GATC 3 cut(s) 193, 423, 973
EaeI YGGCCR 2 cut(s) 22, 664
Eam1104I CTCTTC 3 cut(s) 387, 861, 1034
EarI CTCTTC 3 cut(s) 387, 861, 1034
Eco130I CCWWGG 2 cut(s) 695, 1141
Eco147I AGGCCT 1 cut(s) 823
Eco31I GGTCTC 1 cut(s) 457
Eco57I CTGAAG 1 cut(s) 195
Eco91I GGTNACC 1 cut(s) 57
EcoO65I GGTNACC 1 cut(s) 57
EcoRII CCWGG 3 cut(s) 61, 632, 921
EcoT14I CCWWGG 2 cut(s) 695, 1141
ErhI CCWWGG 2 cut(s) 695, 1141
Esp3I CGTCTC 1 cut(s) 1099
FaeI CATG 1 cut(s) 1105
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FatI CATG 1 cut(s) 1101
FauI CCCGC 1 cut(s) 43
FbaI TGATCA 1 cut(s) 423
FblI GTMKAC 1 cut(s) 691
Fnu4HI GCNGC 3 cut(s) 171, 783, 786
FokI GGATG 3 cut(s) 545, 971, 1109
Fsp4HI GCNGC 3 cut(s) 171, 783, 786
GlaI GCGC 1 cut(s) 102
GluI GCNGC 3 cut(s) 171, 783, 786
GsaI CCCAGC 1 cut(s) 227
GsuI CTGGAG 1 cut(s) 702
HaeIII GGCC 4 cut(s) 24, 666, 823, 965
HapII CCGG 1 cut(s) 1094
HhaI GCGC 1 cut(s) 103
Hin1II CATG 1 cut(s) 1105
Hin6I GCGC 1 cut(s) 101
HinP1I GCGC 1 cut(s) 101
HincII GTYRAC 2 cut(s) 888, 1054
HindII GTYRAC 2 cut(s) 888, 1054
HindIII AAGCTT 1 cut(s) 940
HinfI GANTC 4 cut(s) 208, 455, 559, 656
HpaI GTTAAC 1 cut(s) 888
HpaII CCGG 1 cut(s) 1094
HphI GGTGA 5 cut(s) 51, 80, 776, 853, 869
Hpy166II GTNNAC 6 cut(s) 523, 692, 711, 844, 888, 1054
Hpy188I TCNGA 6 cut(s) 96, 155, 190, 448, 555, 795
Hpy188III TCNNGA 5 cut(s) 113, 199, 627, 896, 1094
Hpy8I GTNNAC 6 cut(s) 523, 692, 711, 844, 888, 1054
HpyAV CCTTC 4 cut(s) 469, 523, 721, 893
HpyCH4III ACNGT 2 cut(s) 715, 1058
HpyCH4IV ACGT 1 cut(s) 88
HpyCH4V TGCA 8 cut(s) 108, 170, 344, 493, 536, 673, 788, 1030
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 30, 782
HpyF3I CTNAG 5 cut(s) 447, 453, 612, 943, 969
HpySE526I ACGT 1 cut(s) 88
Hsp92II CATG 1 cut(s) 1105
HspAI GCGC 1 cut(s) 101
Kpn2I TCCGGA 1 cut(s) 1093
Ksp22I TGATCA 1 cut(s) 423
KspAI GTTAAC 1 cut(s) 888
Kzo9I GATC 3 cut(s) 193, 423, 973
LmnI GCTCC 1 cut(s) 683
Lsp1109I GCAGC 3 cut(s) 182, 769, 772
LweI GCATC 2 cut(s) 523, 682
MaeII ACGT 1 cut(s) 88
MaeIII GTNAC 4 cut(s) 57, 296, 802, 1135
MalI GATC 3 cut(s) 195, 425, 975
MboI GATC 3 cut(s) 193, 423, 973
MflI RGATCY 1 cut(s) 973
MlsI TGGCCA 1 cut(s) 666
MluCI AATT 9 cut(s) 234, 248, 290, 356, 429, 537, 580, 604, 891
MluNI TGGCCA 1 cut(s) 666
MlyI GAGTC 2 cut(s) 464, 665
MmeI TCCRAC 1 cut(s) 386
Mox20I TGGCCA 1 cut(s) 666
MroI TCCGGA 1 cut(s) 1093
MscI TGGCCA 1 cut(s) 666
MseI TTAA 3 cut(s) 851, 887, 1156
MslI CAYNNNNRTG 1 cut(s) 762
Msp20I TGGCCA 1 cut(s) 666
MspA1I CMGCKG 2 cut(s) 33, 173
MspI CCGG 1 cut(s) 1094
MspR9I CCNGG 3 cut(s) 63, 634, 923
Mva1269I GAATGC 1 cut(s) 344
MvaI CCWGG 3 cut(s) 63, 634, 923
MwoI GCNNNNNNNGC 3 cut(s) 15, 30, 782
NdeII GATC 3 cut(s) 193, 423, 973
NlaIII CATG 1 cut(s) 1105
NlaIV GGNNCC 1 cut(s) 631
NmeAIII GCCGAG 1 cut(s) 50
NmuCI GTSAC 2 cut(s) 57, 296
NspV TTCGAA 1 cut(s) 720
PceI AGGCCT 1 cut(s) 823
PctI GAATGC 1 cut(s) 344
PfeI GAWTC 2 cut(s) 208, 559
PflFI GACNNNGTC 1 cut(s) 454
PfoI TCCNGGA 1 cut(s) 921
PkrI GCNGC 3 cut(s) 172, 784, 787
PleI GAGTC 2 cut(s) 463, 664
PpsI GAGTC 2 cut(s) 463, 664
Psp6I CCWGG 3 cut(s) 61, 632, 921
PspEI GGTNACC 1 cut(s) 57
PspFI CCCAGC 1 cut(s) 223
PspGI CCWGG 3 cut(s) 61, 632, 921
PspN4I GGNNCC 1 cut(s) 631
PsuI RGATCY 1 cut(s) 973
PsyI GACNNNGTC 1 cut(s) 454
PvuII CAGCTG 1 cut(s) 173
RsaI GTAC 4 cut(s) 269, 548, 1060, 1122
RsaNI GTAC 4 cut(s) 268, 547, 1059, 1121
RseI CAYNNNNRTG 1 cut(s) 762
SaqAI TTAA 3 cut(s) 851, 887, 1156
SatI GCNGC 3 cut(s) 171, 783, 786
Sau3AI GATC 3 cut(s) 193, 423, 973
SchI GAGTC 2 cut(s) 464, 665
ScrFI CCNGG 3 cut(s) 63, 634, 923
SfaNI GCATC 2 cut(s) 523, 682
SfuI TTCGAA 1 cut(s) 720
SmiMI CAYNNNNRTG 1 cut(s) 762
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 9 cut(s) 234, 248, 290, 356, 429, 537, 580, 604, 891
SseBI AGGCCT 1 cut(s) 823
SsiI CCGC 3 cut(s) 33, 50, 1084
StuI AGGCCT 1 cut(s) 823
StyD4I CCNGG 3 cut(s) 61, 632, 921
StyI CCWWGG 2 cut(s) 695, 1141
TaaI ACNGT 2 cut(s) 715, 1058
TaiI ACGT 1 cut(s) 91
TaqI TCGA 5 cut(s) 112, 585, 720, 897, 1065
TaqII GACCGA 1 cut(s) 1039
TasI AATT 9 cut(s) 234, 248, 290, 356, 429, 537, 580, 604, 891
TfiI GAWTC 2 cut(s) 208, 559
Tru1I TTAA 3 cut(s) 851, 887, 1156
Tru9I TTAA 3 cut(s) 851, 887, 1156
TscAI CASTG 2 cut(s) 172, 718
TseFI GTSAC 2 cut(s) 57, 296
TseI GCWGC 3 cut(s) 170, 782, 785
Tsp45I GTSAC 2 cut(s) 57, 296
TspDTI ATGAA 3 cut(s) 740, 1013, 1023
TspRI CASTG 2 cut(s) 172, 718
Tth111I GACNNNGTC 1 cut(s) 454
XapI RAATTY 3 cut(s) 290, 356, 604
XmiI GTMKAC 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.