Rorug04G0005500

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0014710
Physical Location & Seq
Reverse (-)
977554 .. 978676
1123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0005500.1

Sequence Viewer

Length: 825 bp
ATGATTCTGAAACCGGTGGTTGGTCACCCTTTCCGTGGGGTTGCTGTCGCTTCGGGTCCCAAATTCTCTTCACCGGTGGCAACTGAGGCGCACGCGACTCCATTTACGGCAAGTCGACCGAACTATCACCACTATACCGAGGCTCCTGCTTTTGTAGTATTCGACCCCGAAGGTGATGGAGAGTTTGAGGCCAAATGGTTGCCTCAGGAGTCGCCTCCTCAGGCGATACTCTATGATGAGAAATCCCACAAGCTGAACGAGTTTATGTATTGTGCTGTTGATTCCAAAATCTTAGTCTGGAATCAAGATAAGGCCGGGGTTTACCTCTTTGACGTGAGCCGACCCGAGAAAGGATGGATAGAGTTGCACTCTCCTCTAGCAACCACTCTTCCCTTTGTTAAGGGTTGTTGGGGTGACACTGCAAAGCTCGCCGATCATCATGGTGGTGGAGGAACCCTAATATTTACCTTTGACTGTACAAGAGGATGTAAACTGCCGGAAATCCCTCCTGAATTTCGCAATGATCCATGCGTGAGAGCAGTTGGTCCAACCCAGTGTTGTCTTGTGCATCTAGGAGATCACAAAGTGTGCCTTATTCTTCACAAATATTTCTATGCATTATACCGTGACACAAATGATTTGGAGGAAGGTTACGAACTAGTTGATCATACCGGCAGAAACAAGGGGATTCTTCATCTCATGACGTTTGAATATCAAGTTACTCCCGACTTGGACATCGAGTACCGACTTCTCACTACTCGCAACTTAGAATACTATACCAAGCTGCCTAACAATGAATATGCAACGTATACCAAGCCTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

274

Amino Acids

31.0

Weight (kDa)

5.79

Isoelectric Point (pI)

48.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014710)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06290 FvH4_4g06330
malus_domestica MD13G1235000.v1.1
prunus_persica Prupe.1G066100_v2.0.a1 Prupe.1G066200_v2.0.a1
pyrus_communis pycom13g20750
rosa_chinensis RchiOBHm_Chr4g0398011
rosa_laevigata RLG00000014125
rosa_roxburghii Rroxscaffold_5G00342970
rosa_rugosa Rorug04G0005500 Rorug06G0022700
rosa_samantha Rh4AG082200 Rh4BG079700 Rh4DG075100 Rh6BG144300 Rh6DG128700
rosa_wichuraiana Rw4G006860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 115, 809
AccII CGCG 1 cut(s) 95
AclWI GGATC 1 cut(s) 518
AcsI RAATTY 2 cut(s) 62, 512
AdeI CACNNNGTG 1 cut(s) 586
AfaI GTAC 2 cut(s) 478, 743
AfiI CCNNNNNNNGG 3 cut(s) 20, 35, 350
AgeI ACCGGT 2 cut(s) 13, 73
AgsI TTSAA 1 cut(s) 710
AhlI ACTAGT 1 cut(s) 658
AjiI CACGTC 1 cut(s) 334
AluBI AGCT 3 cut(s) 253, 427, 784
AluI AGCT 3 cut(s) 253, 427, 784
AlwI GGATC 1 cut(s) 518
Ama87I CYCGRG 1 cut(s) 344
AoxI GGCC 2 cut(s) 189, 312
ApeKI GCWGC 1 cut(s) 784
ApoI RAATTY 2 cut(s) 62, 512
ArsI GACNNNNNNTTYG 2 cut(s) 279, 311
AsiGI ACCGGT 2 cut(s) 13, 73
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 2 cut(s) 56, 545
AsuC2I CCSGG 1 cut(s) 316
AsuHPI GGTGA 5 cut(s) 17, 63, 119, 185, 425
AvaI CYCGRG 1 cut(s) 344
AvaII GGWCC 2 cut(s) 56, 545
AxyI CCTNAGG 2 cut(s) 204, 219
BbvI GCAGC 1 cut(s) 771
BccI CCATC 2 cut(s) 170, 348
BceAI ACGGC 1 cut(s) 123
BcgI CGANNNNNNTGC 2 cut(s) 128, 162
BclI TGATCA 1 cut(s) 664
BcnI CCSGG 1 cut(s) 316
BcuI ACTAGT 1 cut(s) 658
BfaI CTAG 3 cut(s) 377, 572, 659
BisI GCNGC 1 cut(s) 785
BlsI GCNGC 1 cut(s) 786
Bme1390I CCNGG 1 cut(s) 316
Bme18I GGWCC 2 cut(s) 56, 545
BmeT110I CYCGRG 1 cut(s) 344
BmgBI CACGTC 1 cut(s) 334
BmgT120I GGNCC 2 cut(s) 56, 545
BmiI GGNNCC 4 cut(s) 57, 58, 144, 454
BmrFI CCNGG 1 cut(s) 316
BmrI ACTGGG 1 cut(s) 547
BmsI GCATC 1 cut(s) 577
BmuI ACTGGG 1 cut(s) 547
BplI GAGNNNNNCTC 2 cut(s) 353, 385
BpuMI CCSGG 1 cut(s) 316
BsaJI CCNNGG 3 cut(s) 34, 138, 315
BsaWI WCCGGW 2 cut(s) 13, 73
BsaXI ACNNNNNCTCC 2 cut(s) 127, 157
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 35, 350
Bse118I RCCGGY 3 cut(s) 13, 73, 671
Bse1I ACTGG 1 cut(s) 553
Bse21I CCTNAGG 2 cut(s) 204, 219
Bse3DI GCAATG 1 cut(s) 526
BseDI CCNNGG 3 cut(s) 34, 138, 315
BseGI GGATG 2 cut(s) 359, 491
BseLI CCNNNNNNNGG 3 cut(s) 20, 35, 350
BseMI GCAATG 1 cut(s) 526
BseMII CTCAG 3 cut(s) 75, 218, 233
BseNI ACTGG 1 cut(s) 553
BseRI GAGGAG 2 cut(s) 207, 363
BseXI GCAGC 1 cut(s) 771
Bsh1236I CGCG 1 cut(s) 95
Bsh1285I CGRYCG 1 cut(s) 119
BshFI GGCC 2 cut(s) 191, 314
BshTI ACCGGT 2 cut(s) 13, 73
BsiEI CGRYCG 1 cut(s) 119
BsiHKCI CYCGRG 1 cut(s) 344
BsiSI CCGG 5 cut(s) 14, 74, 315, 497, 672
BslFI GGGAC 1 cut(s) 42
BslI CCNNNNNNNGG 3 cut(s) 20, 35, 350
BsmFI GGGAC 1 cut(s) 42
BsnI GGCC 2 cut(s) 191, 314
BsoBI CYCGRG 1 cut(s) 344
Bsp1407I TGTACA 1 cut(s) 476
Bsp143I GATC 4 cut(s) 433, 523, 577, 664
BspANI GGCC 2 cut(s) 191, 314
BspCNI CTCAG 3 cut(s) 76, 217, 232
BspFNI CGCG 1 cut(s) 95
BspHI TCATGA 1 cut(s) 699
BspLI GGNNCC 4 cut(s) 57, 58, 144, 454
BspPI GGATC 1 cut(s) 518
BsrDI GCAATG 1 cut(s) 526
BsrFI RCCGGY 3 cut(s) 13, 73, 671
BsrGI TGTACA 1 cut(s) 476
BsrI ACTGG 1 cut(s) 553
BssAI RCCGGY 3 cut(s) 13, 73, 671
BssECI CCNNGG 3 cut(s) 34, 138, 315
BssMI GATC 4 cut(s) 433, 523, 577, 664
BssNAI GTATAC 1 cut(s) 810
Bst1107I GTATAC 1 cut(s) 810
Bst4CI ACNGT 2 cut(s) 476, 626
Bst6I CTCTTC 2 cut(s) 73, 393
BstAUI TGTACA 1 cut(s) 476
BstC8I GCNNGC 2 cut(s) 93, 429
BstDEI CTNAG 5 cut(s) 84, 204, 219, 292, 766
BstDSI CCRYGG 1 cut(s) 34
BstEII GGTNACC 1 cut(s) 23
BstF5I GGATG 2 cut(s) 359, 491
BstFNI CGCG 1 cut(s) 95
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 4 cut(s) 436, 526, 580, 667
BstMBI GATC 4 cut(s) 433, 523, 577, 664
BstMCI CGRYCG 1 cut(s) 119
BstMWI GCNNNNNNNGC 2 cut(s) 86, 428
BstPI GGTNACC 1 cut(s) 23
BstSCI CCNGG 1 cut(s) 314
BstUI CGCG 1 cut(s) 95
BstV1I GCAGC 1 cut(s) 771
BstZ17I GTATAC 1 cut(s) 810
Bsu36I CCTNAGG 2 cut(s) 204, 219
BsuRI GGCC 2 cut(s) 191, 314
BtgI CCRYGG 1 cut(s) 34
BtrI CACGTC 1 cut(s) 334
BtsCI GGATG 2 cut(s) 359, 491
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 2 cut(s) 417, 560
Cac8I GCNNGC 2 cut(s) 93, 429
CciI TCATGA 1 cut(s) 699
CfoI GCGC 1 cut(s) 91
Cfr10I RCCGGY 3 cut(s) 13, 73, 671
Cfr13I GGNCC 2 cut(s) 56, 545
Csp6I GTAC 2 cut(s) 477, 742
CspAI ACCGGT 2 cut(s) 13, 73
CviAII CATG 3 cut(s) 440, 528, 700
CviJI RGCY 8 cut(s) 143, 191, 253, 314, 339, 427, 784, 817
CviKI_1 RGCY 8 cut(s) 143, 191, 253, 314, 339, 427, 784, 817
CviQI GTAC 2 cut(s) 477, 742
DdeI CTNAG 5 cut(s) 84, 204, 219, 292, 766
DpnI GATC 4 cut(s) 435, 525, 579, 666
DpnII GATC 4 cut(s) 433, 523, 577, 664
DraIII CACNNNGTG 1 cut(s) 586
Eam1104I CTCTTC 2 cut(s) 73, 393
EarI CTCTTC 2 cut(s) 73, 393
Eco47I GGWCC 2 cut(s) 56, 545
Eco81I CCTNAGG 2 cut(s) 204, 219
Eco88I CYCGRG 1 cut(s) 344
Eco91I GGTNACC 1 cut(s) 23
EcoO109I RGGNCCY 1 cut(s) 56
EcoO65I GGTNACC 1 cut(s) 23
EcoT22I ATGCAT 1 cut(s) 619
FaeI CATG 3 cut(s) 443, 531, 703
FalI AAGNNNNNCTT 2 cut(s) 576, 608
FaqI GGGAC 1 cut(s) 42
FatI CATG 3 cut(s) 439, 527, 699
FbaI TGATCA 1 cut(s) 664
FblI GTMKAC 2 cut(s) 115, 809
Fnu4HI GCNGC 1 cut(s) 785
FokI GGATG 2 cut(s) 366, 498
Fsp4HI GCNGC 1 cut(s) 785
FspBI CTAG 3 cut(s) 377, 572, 659
GlaI GCGC 1 cut(s) 90
GluI GCNGC 1 cut(s) 785
HaeIII GGCC 2 cut(s) 191, 314
HapII CCGG 5 cut(s) 14, 74, 315, 497, 672
HhaI GCGC 1 cut(s) 91
Hin1II CATG 3 cut(s) 443, 531, 703
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HincII GTYRAC 1 cut(s) 116
HindII GTYRAC 1 cut(s) 116
HinfI GANTC 6 cut(s) 4, 97, 209, 281, 301, 688
HpaII CCGG 5 cut(s) 14, 74, 315, 497, 672
HphI GGTGA 5 cut(s) 17, 63, 119, 185, 425
Hpy166II GTNNAC 4 cut(s) 116, 322, 491, 810
Hpy188I TCNGA 1 cut(s) 9
Hpy188III TCNNGA 6 cut(s) 206, 298, 305, 509, 700, 725
Hpy8I GTNNAC 4 cut(s) 116, 322, 491, 810
HpyAV CCTTC 2 cut(s) 164, 641
HpyCH4III ACNGT 2 cut(s) 476, 626
HpyCH4IV ACGT 3 cut(s) 333, 704, 806
HpyCH4V TGCA 5 cut(s) 367, 422, 568, 617, 803
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 428
HpyF3I CTNAG 5 cut(s) 84, 204, 219, 292, 766
HpySE526I ACGT 3 cut(s) 333, 704, 806
Hsp92II CATG 3 cut(s) 443, 531, 703
HspAI GCGC 1 cut(s) 89
KflI GGGWCCC 1 cut(s) 56
Ksp22I TGATCA 1 cut(s) 664
Kzo9I GATC 4 cut(s) 433, 523, 577, 664
LmnI GCTCC 1 cut(s) 148
Lsp1109I GCAGC 1 cut(s) 771
LweI GCATC 1 cut(s) 577
MaeI CTAG 3 cut(s) 377, 572, 659
MaeII ACGT 3 cut(s) 333, 704, 806
MaeIII GTNAC 5 cut(s) 23, 413, 626, 650, 718
MalI GATC 4 cut(s) 435, 525, 579, 666
MboI GATC 4 cut(s) 433, 523, 577, 664
MboII GAAGA 4 cut(s) 60, 380, 590, 683
MluCI AATT 2 cut(s) 62, 512
MlyI GAGTC 2 cut(s) 91, 218
MmeI TCCRAC 1 cut(s) 572
Mph1103I ATGCAT 1 cut(s) 619
MseI TTAA 1 cut(s) 399
MslI CAYNNNNRTG 2 cut(s) 441, 444
MspI CCGG 5 cut(s) 14, 74, 315, 497, 672
MspR9I CCNGG 1 cut(s) 316
MvnI CGCG 1 cut(s) 95
MwoI GCNNNNNNNGC 2 cut(s) 86, 428
NciI CCSGG 1 cut(s) 316
NdeII GATC 4 cut(s) 433, 523, 577, 664
NlaIII CATG 3 cut(s) 443, 531, 703
NlaIV GGNNCC 4 cut(s) 57, 58, 144, 454
NmuCI GTSAC 3 cut(s) 23, 413, 626
NsiI ATGCAT 1 cut(s) 619
PagI TCATGA 1 cut(s) 699
PfeI GAWTC 4 cut(s) 4, 281, 301, 688
PinAI ACCGGT 2 cut(s) 13, 73
PkrI GCNGC 1 cut(s) 786
PleI GAGTC 2 cut(s) 91, 217
PpsI GAGTC 2 cut(s) 91, 217
PpuMI RGGWCCY 1 cut(s) 56
Psp5II RGGWCCY 1 cut(s) 56
PspEI GGTNACC 1 cut(s) 23
PspN4I GGNNCC 4 cut(s) 57, 58, 144, 454
PspPI GGNCC 2 cut(s) 56, 545
PspPPI RGGWCCY 1 cut(s) 56
RsaI GTAC 2 cut(s) 478, 743
RsaNI GTAC 2 cut(s) 477, 742
RseI CAYNNNNRTG 2 cut(s) 441, 444
SalI GTCGAC 1 cut(s) 114
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 1 cut(s) 785
Sau3AI GATC 4 cut(s) 433, 523, 577, 664
Sau96I GGNCC 2 cut(s) 56, 545
SchI GAGTC 2 cut(s) 91, 218
ScrFI CCNGG 1 cut(s) 316
SfaNI GCATC 1 cut(s) 577
SgrAI CRCCGGYG 1 cut(s) 73
SinI GGWCC 2 cut(s) 56, 545
SmiMI CAYNNNNRTG 2 cut(s) 441, 444
SpeI ACTAGT 1 cut(s) 658
Sse9I AATT 2 cut(s) 62, 512
SspI AATATT 2 cut(s) 462, 608
SspMI CTAG 3 cut(s) 377, 572, 659
StyD4I CCNGG 1 cut(s) 314
TaaI ACNGT 2 cut(s) 476, 626
TaiI ACGT 3 cut(s) 336, 707, 809
TaqI TCGA 3 cut(s) 115, 162, 738
TaqII GACCGA 1 cut(s) 133
TasI AATT 2 cut(s) 62, 512
TatI WGTACW 1 cut(s) 476
TfiI GAWTC 4 cut(s) 4, 281, 301, 688
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TscAI CASTG 2 cut(s) 424, 560
TseFI GTSAC 3 cut(s) 23, 413, 626
TseI GCWGC 1 cut(s) 784
Tsp45I GTSAC 3 cut(s) 23, 413, 626
TspDTI ATGAA 2 cut(s) 683, 810
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 2 cut(s) 424, 560
VpaK11BI GGWCC 2 cut(s) 56, 545
XapI RAATTY 2 cut(s) 62, 512
XmiI GTMKAC 2 cut(s) 115, 809
XspI CTAG 3 cut(s) 377, 572, 659
Zsp2I ATGCAT 1 cut(s) 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.