MD13G1235000.v1.1

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014710
Physical Location & Seq
Forward (+)
23647060 .. 23648271
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1235000.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGGCACCGGAGGCTAAAAGCAGAGCGAGAGGGGGAGGAGGAAGAAGCGGCAAAGCCACAGATTCCGGTGCTGCAGCTGCCGTGGCCAGCGCAGTTGACCTCCTGCTAGAGATCCTGATGCGCCTCCCGGCAAGACCCCTTCACCGATTCAAATGCGTATCGAAACAATGGTACTCTCTCATCTCTGACCCCCAATTTAGAATCGATTACGTCCAAAAGAACCCTAATCATCTTTTGGGTTTGCTGCTGATTATTCAAGGTCCATACTCTGAATTTTCGTTCATTTCCCTTGGAGGCTGTGGCCAAATTGTTAAAGACCCAAAGTTTTTAAGCACCACCAATCTCAAAATTCATCATTTCTGTAATGGGTTGATGTGTTTTTCCAAGCCTCACAAGGATTCACCTCAGTTTACTTCTTATTATGTTTGTAATTCTGCTTCCGGGCACTCGAAAAGAATTCGGGTTCTTGAGAGCTCGACCAGAAAGAGATTGATGGCTGTTAATGTGGCTTTTGATCCTTCAAAGTCACCCTTCTACAATGTCGTTTTTGTTAGTGAAGAATCCGGGTCGGAACTGAACCATCTGATCAAAATTGATATATACTCATCCGAGACCGAGGCTTGGAGACCCAGCAGCAAGGGGCTGTACATTGTGCCTTACGATGTGGATTTTGACAATGGGGTTTACTGGAATGGGGCAATTTATTGGTACCATCAAAGGAAAAACTCTTTGGCGTATTTTGATTTGGATACCGGTTGCCTTGAAAGATTACGAATGCCACTGCTTTCTCTGGGATCGTATCCCATGGTTTTCGACCATTTTGGAGAATCAGGTGGCCATCTGCATCTTGTTGGGATCTGCAGTCCAAGGACTACTCTGTTCACTGTGTTTGAATTGCAGCAGGATAGGTCCGGTTGGTTTGTGAAGTGTAATGGTGATTTGGATGCCGTAGCAGCTGCTTTCCCAAAGATGGTTACCCTTAAACCCAGTAACCCTTATGTGTTTTCTGCAGTGAGCCTTGTTAGAGAAGAAAAAGAAGAGGAACTGAGTATTGTGCTAACGATACCTGGGAGTGTGGTCAGATATGATGTCAAGCATAAGACATCAAGGAAGCTCTGTGATCTTCCAAAAGGCTGTATGTTCAATGACTTTATGGCTATACAGAGGAGGGTTAAGGCGCATCAATACATTGAGACCCTTGATTCCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

404

Amino Acids

45.28

Weight (kDa)

8.86

Isoelectric Point (pI)

41.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 33 - 68 2.2e-08 F-box domain
FBA_1 PF07734 116 - 261 1.8e-10 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014710)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06290 FvH4_4g06330
malus_domestica MD13G1235000.v1.1
prunus_persica Prupe.1G066100_v2.0.a1 Prupe.1G066200_v2.0.a1
pyrus_communis pycom13g20750
rosa_chinensis RchiOBHm_Chr4g0398011
rosa_laevigata RLG00000014125
rosa_roxburghii Rroxscaffold_5G00342970
rosa_rugosa Rorug04G0005500 Rorug06G0022700
rosa_samantha Rh4AG082200 Rh4BG079700 Rh4DG075100 Rh6BG144300 Rh6DG128700
rosa_wichuraiana Rw4G006860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 708
AccB1I GGYRCC 2 cut(s) 4, 708
AciI CCGC 1 cut(s) 48
AclWI GGATC 4 cut(s) 106, 509, 802, 863
AcoI YGGCCR 3 cut(s) 84, 301, 835
AcsI RAATTY 3 cut(s) 272, 348, 456
AfaI GTAC 3 cut(s) 173, 647, 710
AfiI CCNNNNNNNGG 2 cut(s) 621, 970
AgeI ACCGGT 1 cut(s) 752
AgsI TTSAA 6 cut(s) 151, 257, 522, 764, 893, 1144
AjnI CCWGG 1 cut(s) 1066
AjuI GAANNNNNNNTTGG 2 cut(s) 713, 745
AluBI AGCT 4 cut(s) 77, 474, 956, 1114
AluI AGCT 4 cut(s) 77, 474, 956, 1114
Alw21I GWGCWC 1 cut(s) 476
Alw26I GTCTC 3 cut(s) 605, 619, 1187
AlwI GGATC 4 cut(s) 106, 509, 802, 863
AoxI GGCC 3 cut(s) 84, 301, 835
ApeKI GCWGC 8 cut(s) 71, 74, 77, 244, 633, 898, 953, 956
ApoI RAATTY 3 cut(s) 272, 348, 456
AsiGI ACCGGT 1 cut(s) 752
Asp718I GGTACC 1 cut(s) 708
AspLEI GCGC 3 cut(s) 92, 123, 1180
AspS9I GGNCC 2 cut(s) 260, 909
AsuC2I CCSGG 3 cut(s) 128, 442, 565
AsuHPI GGTGA 4 cut(s) 134, 393, 519, 947
AvaII GGWCC 2 cut(s) 260, 909
BaeGI GKGCMC 1 cut(s) 447
BalI TGGCCA 3 cut(s) 86, 303, 837
BanI GGYRCC 2 cut(s) 4, 708
BanII GRGCYC 1 cut(s) 476
BarI GAAGNNNNNNTAC 2 cut(s) 421, 453
Bbv12I GWGCWC 1 cut(s) 476
BbvI GCAGC 8 cut(s) 58, 64, 86, 231, 645, 910, 943, 965
BccI CCATC 5 cut(s) 487, 588, 720, 846, 964
BceAI ACGGC 2 cut(s) 65, 932
BcgI CGANNNNNNTGC 2 cut(s) 135, 169
BciT130I CCWGG 1 cut(s) 1068
BciVI GTATCC 2 cut(s) 742, 810
BclI TGATCA 1 cut(s) 585
BcnI CCSGG 3 cut(s) 128, 442, 565
BcoDI GTCTC 3 cut(s) 605, 619, 1187
BfaI CTAG 1 cut(s) 107
BfmI CTRYAG 3 cut(s) 72, 859, 1008
BfuI GTATCC 2 cut(s) 742, 810
BisI GCNGC 9 cut(s) 49, 72, 75, 78, 245, 634, 899, 954, 957
BlsI GCNGC 9 cut(s) 50, 73, 76, 79, 246, 635, 900, 955, 958
Bme1390I CCNGG 4 cut(s) 128, 442, 565, 1068
Bme18I GGWCC 2 cut(s) 260, 909
BmgT120I GGNCC 2 cut(s) 260, 909
BmiI GGNNCC 2 cut(s) 6, 710
BmrFI CCNGG 4 cut(s) 128, 442, 565, 1068
BmrI ACTGGG 1 cut(s) 981
BmsI GCATC 4 cut(s) 108, 853, 934, 1189
BmuI ACTGGG 1 cut(s) 981
BpuEI CTTGAG 1 cut(s) 488
BpuMI CCSGG 3 cut(s) 128, 442, 565
Bsa29I ATCGAT 1 cut(s) 204
BsaI GGTCTC 3 cut(s) 605, 619, 1187
BsaJI CCNNGG 6 cut(s) 81, 289, 615, 804, 866, 1067
BsaWI WCCGGW 4 cut(s) 7, 65, 752, 911
Bsc4I CCNNNNNNNGG 2 cut(s) 621, 970
Bse118I RCCGGY 1 cut(s) 752
Bse1I ACTGG 2 cut(s) 692, 987
BseBI CCWGG 1 cut(s) 1068
BseCI ATCGAT 1 cut(s) 204
BseDI CCNNGG 6 cut(s) 81, 289, 615, 804, 866, 1067
BseGI GGATG 2 cut(s) 605, 949
BseLI CCNNNNNNNGG 2 cut(s) 621, 970
BseMII CTCAG 2 cut(s) 419, 1037
BseNI ACTGG 2 cut(s) 692, 987
BseRI GAGGAG 2 cut(s) 51, 1180
BseSI GKGCMC 1 cut(s) 447
BseXI GCAGC 8 cut(s) 58, 64, 86, 231, 645, 910, 943, 965
BseYI CCCAGC 1 cut(s) 629
BshFI GGCC 3 cut(s) 86, 303, 837
BshNI GGYRCC 2 cut(s) 4, 708
BshTI ACCGGT 1 cut(s) 752
BshVI ATCGAT 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 476
BsiSI CCGG 7 cut(s) 8, 66, 128, 441, 564, 753, 912
BslI CCNNNNNNNGG 2 cut(s) 621, 970
BsmAI GTCTC 3 cut(s) 605, 619, 1187
BsmI GAATGC 1 cut(s) 780
BsnI GGCC 3 cut(s) 86, 303, 837
Bso31I GGTCTC 3 cut(s) 605, 619, 1187
Bsp1286I GDGCHC 2 cut(s) 447, 476
Bsp1407I TGTACA 1 cut(s) 645
Bsp143I GATC 6 cut(s) 111, 514, 585, 794, 855, 1120
Bsp19I CCATGG 1 cut(s) 804
BspACI CCGC 1 cut(s) 48
BspANI GGCC 3 cut(s) 86, 303, 837
BspCNI CTCAG 2 cut(s) 418, 1038
BspDI ATCGAT 1 cut(s) 204
BspLI GGNNCC 2 cut(s) 6, 710
BspMAI CTGCAG 3 cut(s) 76, 863, 1012
BspPI GGATC 4 cut(s) 106, 509, 802, 863
BspT107I GGYRCC 2 cut(s) 4, 708
BspTNI GGTCTC 3 cut(s) 605, 619, 1187
BsrFI RCCGGY 1 cut(s) 752
BsrGI TGTACA 1 cut(s) 645
BsrI ACTGG 2 cut(s) 692, 987
BssAI RCCGGY 1 cut(s) 752
BssECI CCNNGG 6 cut(s) 81, 289, 615, 804, 866, 1067
BssMI GATC 6 cut(s) 111, 514, 585, 794, 855, 1120
BssT1I CCWWGG 3 cut(s) 289, 804, 866
Bst2UI CCWGG 1 cut(s) 1068
Bst4CI ACNGT 1 cut(s) 886
Bst6I CTCTTC 1 cut(s) 1032
BstAUI TGTACA 1 cut(s) 645
BstC8I GCNNGC 1 cut(s) 88
BstDEI CTNAG 2 cut(s) 405, 1046
BstDSI CCRYGG 2 cut(s) 81, 804
BstEII GGTNACC 1 cut(s) 973
BstF5I GGATG 2 cut(s) 605, 949
BstHHI GCGC 3 cut(s) 92, 123, 1180
BstKTI GATC 6 cut(s) 114, 517, 588, 797, 858, 1123
BstMAI GTCTC 3 cut(s) 605, 619, 1187
BstMBI GATC 6 cut(s) 111, 514, 585, 794, 855, 1120
BstMWI GCNNNNNNNGC 4 cut(s) 11, 77, 83, 953
BstNI CCWGG 1 cut(s) 1068
BstPI GGTNACC 1 cut(s) 973
BstSCI CCNGG 4 cut(s) 126, 440, 563, 1066
BstSFI CTRYAG 3 cut(s) 72, 859, 1008
BstSLI GKGCMC 1 cut(s) 447
BstV1I GCAGC 8 cut(s) 58, 64, 86, 231, 645, 910, 943, 965
BstX2I RGATCY 2 cut(s) 111, 855
BstYI RGATCY 2 cut(s) 111, 855
Bsu15I ATCGAT 1 cut(s) 204
BsuI GTATCC 2 cut(s) 742, 810
BsuRI GGCC 3 cut(s) 86, 303, 837
BsuTUI ATCGAT 1 cut(s) 204
BtgI CCRYGG 2 cut(s) 81, 804
BtsCI GGATG 2 cut(s) 605, 949
BtsI GCAGTG 2 cut(s) 779, 1017
BtsIMutI CAGTG 3 cut(s) 779, 882, 1017
Cac8I GCNNGC 1 cut(s) 88
CfoI GCGC 3 cut(s) 92, 123, 1180
Cfr10I RCCGGY 1 cut(s) 752
Cfr13I GGNCC 2 cut(s) 260, 909
ClaI ATCGAT 1 cut(s) 204
Csp6I GTAC 3 cut(s) 172, 646, 709
CspAI ACCGGT 1 cut(s) 752
CviAII CATG 1 cut(s) 805
CviQI GTAC 3 cut(s) 172, 646, 709
DdeI CTNAG 2 cut(s) 405, 1046
DpnI GATC 6 cut(s) 113, 516, 587, 796, 857, 1122
DpnII GATC 6 cut(s) 111, 514, 585, 794, 855, 1120
EaeI YGGCCR 3 cut(s) 84, 301, 835
Eam1104I CTCTTC 1 cut(s) 1032
EarI CTCTTC 1 cut(s) 1032
Ecl136II GAGCTC 1 cut(s) 474
Eco130I CCWWGG 3 cut(s) 289, 804, 866
Eco24I GRGCYC 1 cut(s) 476
Eco31I GGTCTC 3 cut(s) 605, 619, 1187
Eco47I GGWCC 2 cut(s) 260, 909
Eco53kI GAGCTC 1 cut(s) 474
Eco91I GGTNACC 1 cut(s) 973
EcoICRI GAGCTC 1 cut(s) 474
EcoO65I GGTNACC 1 cut(s) 973
EcoRI GAATTC 1 cut(s) 456
EcoRII CCWGG 1 cut(s) 1066
EcoT14I CCWWGG 3 cut(s) 289, 804, 866
EcoT38I GRGCYC 1 cut(s) 476
ErhI CCWWGG 3 cut(s) 289, 804, 866
FaeI CATG 1 cut(s) 808
FalI AAGNNNNNCTT 2 cut(s) 515, 547
FatI CATG 1 cut(s) 804
FbaI TGATCA 1 cut(s) 585
Fnu4HI GCNGC 9 cut(s) 49, 72, 75, 78, 245, 634, 899, 954, 957
FokI GGATG 2 cut(s) 592, 956
FriOI GRGCYC 1 cut(s) 476
Fsp4HI GCNGC 9 cut(s) 49, 72, 75, 78, 245, 634, 899, 954, 957
FspBI CTAG 1 cut(s) 107
GlaI GCGC 3 cut(s) 91, 122, 1179
GluI GCNGC 9 cut(s) 49, 72, 75, 78, 245, 634, 899, 954, 957
GsaI CCCAGC 1 cut(s) 633
HaeIII GGCC 3 cut(s) 86, 303, 837
HapII CCGG 7 cut(s) 8, 66, 128, 441, 564, 753, 912
HhaI GCGC 3 cut(s) 92, 123, 1180
Hin1II CATG 1 cut(s) 808
Hin6I GCGC 3 cut(s) 90, 121, 1178
HinP1I GCGC 3 cut(s) 90, 121, 1178
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HinfI GANTC 7 cut(s) 62, 147, 201, 398, 560, 827, 1202
HpaII CCGG 7 cut(s) 8, 66, 128, 441, 564, 753, 912
HphI GGTGA 4 cut(s) 134, 393, 519, 947
Hpy166II GTNNAC 4 cut(s) 97, 411, 685, 882
Hpy188I TCNGA 7 cut(s) 187, 271, 571, 585, 610, 1082, 1211
Hpy188III TCNNGA 2 cut(s) 115, 467
Hpy8I GTNNAC 4 cut(s) 97, 411, 685, 882
HpyAV CCTTC 3 cut(s) 149, 528, 541
HpyCH4III ACNGT 1 cut(s) 886
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 5 cut(s) 74, 844, 861, 898, 1010
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 77, 83, 953
HpyF3I CTNAG 2 cut(s) 405, 1046
HpySE526I ACGT 1 cut(s) 210
Hsp92II CATG 1 cut(s) 808
HspAI GCGC 3 cut(s) 90, 121, 1178
KpnI GGTACC 1 cut(s) 712
Ksp22I TGATCA 1 cut(s) 585
Kzo9I GATC 6 cut(s) 111, 514, 585, 794, 855, 1120
Lsp1109I GCAGC 8 cut(s) 58, 64, 86, 231, 645, 910, 943, 965
LweI GCATC 4 cut(s) 108, 853, 934, 1189
MaeI CTAG 1 cut(s) 107
MaeII ACGT 1 cut(s) 210
MaeIII GTNAC 3 cut(s) 525, 973, 989
MalI GATC 6 cut(s) 113, 516, 587, 796, 857, 1122
MboI GATC 6 cut(s) 111, 514, 585, 794, 855, 1120
MboII GAAGA 5 cut(s) 54, 569, 1040, 1049, 1115
MflI RGATCY 2 cut(s) 111, 855
MhlI GDGCHC 2 cut(s) 447, 476
MlsI TGGCCA 3 cut(s) 86, 303, 837
MluCI AATT 9 cut(s) 194, 272, 306, 348, 430, 456, 591, 699, 893
MluNI TGGCCA 3 cut(s) 86, 303, 837
MmeI TCCRAC 1 cut(s) 549
Mox20I TGGCCA 3 cut(s) 86, 303, 837
MscI TGGCCA 3 cut(s) 86, 303, 837
MseI TTAA 5 cut(s) 312, 329, 501, 981, 1173
Msp20I TGGCCA 3 cut(s) 86, 303, 837
MspA1I CMGCKG 2 cut(s) 77, 956
MspI CCGG 7 cut(s) 8, 66, 128, 441, 564, 753, 912
MspR9I CCNGG 4 cut(s) 128, 442, 565, 1068
Mva1269I GAATGC 1 cut(s) 780
MvaI CCWGG 1 cut(s) 1068
MwoI GCNNNNNNNGC 4 cut(s) 11, 77, 83, 953
NciI CCSGG 3 cut(s) 128, 442, 565
NcoI CCATGG 1 cut(s) 804
NdeII GATC 6 cut(s) 111, 514, 585, 794, 855, 1120
NlaIII CATG 1 cut(s) 808
NlaIV GGNNCC 2 cut(s) 6, 710
NmuCI GTSAC 1 cut(s) 525
PctI GAATGC 1 cut(s) 780
PfeI GAWTC 7 cut(s) 62, 147, 201, 398, 560, 827, 1202
PinAI ACCGGT 1 cut(s) 752
PkrI GCNGC 9 cut(s) 50, 73, 76, 79, 246, 635, 900, 955, 958
Psp124BI GAGCTC 1 cut(s) 476
Psp6I CCWGG 1 cut(s) 1066
PspEI GGTNACC 1 cut(s) 973
PspFI CCCAGC 1 cut(s) 629
PspGI CCWGG 1 cut(s) 1066
PspN4I GGNNCC 2 cut(s) 6, 710
PspPI GGNCC 2 cut(s) 260, 909
PstI CTGCAG 3 cut(s) 76, 863, 1012
PsuI RGATCY 2 cut(s) 111, 855
PvuII CAGCTG 2 cut(s) 77, 956
RsaI GTAC 3 cut(s) 173, 647, 710
RsaNI GTAC 3 cut(s) 172, 646, 709
SacI GAGCTC 1 cut(s) 476
SaqAI TTAA 5 cut(s) 312, 329, 501, 981, 1173
SatI GCNGC 9 cut(s) 49, 72, 75, 78, 245, 634, 899, 954, 957
Sau3AI GATC 6 cut(s) 111, 514, 585, 794, 855, 1120
Sau96I GGNCC 2 cut(s) 260, 909
ScrFI CCNGG 4 cut(s) 128, 442, 565, 1068
SduI GDGCHC 2 cut(s) 447, 476
SfaNI GCATC 4 cut(s) 108, 853, 934, 1189
SfcI CTRYAG 3 cut(s) 72, 859, 1008
SinI GGWCC 2 cut(s) 260, 909
SmlI CTYRAG 1 cut(s) 467
SmoI CTYRAG 1 cut(s) 467
Sse9I AATT 9 cut(s) 194, 272, 306, 348, 430, 456, 591, 699, 893
SsiI CCGC 1 cut(s) 48
SspMI CTAG 1 cut(s) 107
SstI GAGCTC 1 cut(s) 476
StyD4I CCNGG 4 cut(s) 126, 440, 563, 1066
StyI CCWWGG 3 cut(s) 289, 804, 866
TaaI ACNGT 1 cut(s) 886
TaiI ACGT 1 cut(s) 213
TaqI TCGA 5 cut(s) 161, 204, 449, 476, 813
TaqII GACCGA 1 cut(s) 629
TasI AATT 9 cut(s) 194, 272, 306, 348, 430, 456, 591, 699, 893
TatI WGTACW 1 cut(s) 645
TauI GCSGC 1 cut(s) 51
TfiI GAWTC 7 cut(s) 62, 147, 201, 398, 560, 827, 1202
Tru1I TTAA 5 cut(s) 312, 329, 501, 981, 1173
Tru9I TTAA 5 cut(s) 312, 329, 501, 981, 1173
TscAI CASTG 3 cut(s) 786, 889, 1017
TseFI GTSAC 1 cut(s) 525
TseI GCWGC 8 cut(s) 71, 74, 77, 244, 633, 898, 953, 956
Tsp45I GTSAC 1 cut(s) 525
TspDTI ATGAA 2 cut(s) 271, 341
TspRI CASTG 3 cut(s) 786, 889, 1017
VpaK11BI GGWCC 2 cut(s) 260, 909
XapI RAATTY 3 cut(s) 272, 348, 456
XspI CTAG 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.