Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0009542

Physical Location & Seq

Genomic Coordinates Reverse (-)

15220800 .. 15235160

Gene Structure 14361 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g11470.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3561 bp

ATGGAAGAGGTGTGCGAGGGCAAGGACTTCTCCTTCCCAAAGCAAGAGGAGAAGATTCTCCACTACTGGTCCGAAATCAAAGCCTTCGAGACTCAACTGGCTCTCACCAAGGACCTCCCCGAGTACGTCTTCTACGACGGCCCCCCCTTCGCCACCGGCCTCCCTCACTACGGCCACATACTCGCCGGCACCATCAAGGACATCATCACGCGCTACCAGTCCATGACCGGCCACCACGTCACGCGCCGCTTCGGCTGGGACTGCCACGGCCTCCCCGTCGAGAACGAGATTGATAAGAAGCTCGACATCACGCGCCGCGAACAGATTATGGAAATGGGGATCGGTAAGTACAACGATGCCTGCCGGAGCATTGTTACTCGCTATGTGGAGGAATGGGAGAAGGTTATTACTCGCACTGGGAGATGGATCGATTTTCGGAACGATTATAAGACTATGGACTTGAATTTTATGGAGAGTGTGTGGTGGGTTTTCGCTCAGATTTATGAAAAAGGCCTTGTCTATAAGGGCTTCAAGGTCATGCCTTACAGCACAGGTTGCAAGACGCCGCTCTCCAATTTTGAGGCTAATCAAGATTACAAGGATGTTCCTGATCCTGAAGTTATGGTGGCTTTTCCAATTCTTGGTGATTCAGACGAGGCAAGCTTTGTGGCCTGGACAACTACTCCGTGGACTCTTCCTAGCCATTTAGCACTTTGTGTCAATGCAAATTTTACCTATTTGAAGGTTCGCAACAAGTACTCAAAAAAAGTTTATGTTGTTGCTGAATCTCGGTTGTCAGCTCTTCCTAATGACAAACCAAAAGAAAATGTACCAAATGGATCTGTTGATTCAAAAAAATCAAATTCAAAGAGCAAGGGATCCTCAGGCGGAAAAAAGGAAGCAGTTGACTCGTCATACGAAGTGTTGCAGAAAATGTCAGGGGCTTCATTAGTGGGCACAAAGTATGAACCACCATTTGATTACTTTAAAGAGTTCTCTGATGTTGCATTTAGAGTAGTTGCAGACAACTATGTTACTGATGACAGTGGTACTGGAATTGTCCACTGTGCTCCTGCATTTGGTGAAGACGATTATCGTGTTTGCCTTGAAAATAAAGTTATCAATAAGGGAGAGACCTTGATTGTAGCAGTTGATGAGGATGGATGCTTTACTGAAAAAATTACGGACTTCAGCAAGTGTTATGTTAAAAATGCAGATAAAGATATTATTGAAGCAGTGAAGCGGAAAGGAAGGCTTGTAAAGTCTGGAACCATTATGCATTCTTATCCTCATTGCCCAAGATCTAAAACTCCGCTTATCCAAAGAGCCGTTCCAAGTTGGTTTATCCGGGTGGAGCAGTTGAAAGAAAAATTGTTAGAAAACAACAAGCAGACATACTGGGTTCCTGATTTTGTGAAGGAAAAACGATTCCACAATTGGCTGGAAAATGCAAGAGATTGGGCAGTTAGTCGAAGTAGATTTTGGGGCACTCCTCTCCCTGTGTGGATTAGTGAGGATGGCGAGGAAATAGAAGTTATGGATTCCATTAAGAAGCTCGAAGAGCGTTCTGGTGTTAAGGTTTTTGATCTTCACCGGCACAATATAGATCACATCACTATTCCATCTAGGCGTGGTGCTCAATTTGGTGTTCTTCGACGTATTGATGATGTGTTTGATTGTTGGTTCGAGAGTGGTTCCATGCCTTATGCTTATATCCATTATCCTTTTGAGAATGTTGAACTTTTTGAGAAAAACTTTCCTGGAAATTTTGTGGCTGAAGGGCTGGATCAGACTCGTGGATGGTTCTACACTCTTATGGTGTTATCTACTGCATTATTTGGGAAACCTGCCTTTCAGAATCTCATTTGCAATGGACTTGTACTTGCTGAGGATGGAAAAAAAATGAGTAAAAGTCTGAAAAATTATCCTCCACCAATTGATGTTATCGATCAGTATGGAGCTGATGCAGTACGATTATACCTAATCAACTCTCCTGTTGTGCGCGCGGAACCGTTACGTTTCAAGAAGGAAGGAGTTTATGGTGTTGTCAAGGACGTATTTTTGCCATGGTACAATGCATATAGGTTCCTTGTCCAGAATGCAAAGAGACTTGAGATTGAAGGGTTTGCCCCTTTTGTCCCTATTGATCAAGCCACTCTACAAAAGTCATCTAATGTGCTTGACCAGTGGATCAACTCAGCCACTCAGAGTCTTGTGTATTTTGTCCGTCAGGAGATGAATGGTTACCGACTTTATACGGTGGTTCCATATCTTTTGAAGTTTCTCGACAACTTGACAAATATCTATGTGAGGTGCAATCGCAAGAGGTTGAAGGGCCGTACAGGTGAAGAAGATTGTAGGGTGGCGCTCTCAACACTTTACAATGTCCTTTTGGTATCATGCAAAGCAATGGCCCCATTAACACCATTTTTCACTGAGGTCCTTTTTCAAAATATGAGGAAAGTTTCCAACACAGCAGAGGAAAGCATTCATCATTGCAGCTTTCCTGAAGCAGAAGGCAAGAGGGATGAACGTATAGAGAAAAGTGTTGCTAGGATGATGACGATAATTGATCTTGCCCGTAATATTCGTGAGAGACATAACAAACCTCTGAAAACACCCCTCAGGGAGATGGTTATTGTTCATCCTGATATGGACTTTCTTGATGATATCGCTGGAAAGCTAAAAGAGTATGTGCTGGAGGAACTGAACGTGCGATCTCTTGTTCCATGTAATGATACATTGAAGTATGCCTCATTACGTGCAGAGCCCGATTTCAGCGTTCTGGGCAAGCGGCTAGGAAAACATATGGGAATTGTTGCCAAAGAAGTCAAGGCAATGTCGCAGGAAAGCATTTTAGCATTCGAGAAATCTGGAGAAGTTACTTTTTCTGGTCATTGCTTAAAGCTGACTGATATTAAGGTTGTTAGGGATTTCAAGCGTCCTGATGGCACTGCGGAAACCGAAGTTGATGCAACAGGAGATGGTGATGTTTTGGTGATTTTGGATTTACGTCCGGATGAGTCGTTGTTTGATGCAGGTGTTGCTCGAGAGATTATCAACAGAATACAGAAGTTAAGAAAGAAATCTGCTCTAGAACCTACTGACTTGGTGGAGGTCTACTTTGACTCTTTGGATAAAGAGGATAAAGATAAAGCAGTTTCCGAACGGGTTTTGCAATCACAGGAACAATACATTAGGGATGCCATTGGTTCGCCATTGCTTCCTTCAAGTGTGATGCCATCACATGCTGTCCTTGTTGGGGAAGAGAGTTTCCATGGGATTTCTGGCATTTCATTTAATATCAAACTAGCCAGGCCTGCCTTAGTTTTTAACGCGGACGCAATTGTTGCTTTATATTCAGGAAACTCAGAATTCGCAAGGTGTCTGCAAACTTACTTGTTGTCAAGGGATCATGCAAATCTGAAGTATGAGTTTCAACATGGAAACGGAAAGATAACTGTTGATTGCATTGAAAATTTGCCCGCCGTGAGTTTGGTGTCGAGGGAACATGTGTATTTGACTGTTGGGGAGTTCTTATGTAGAACAAACTCTGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004822 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006428 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0017101 GO:0019538 GO:0019752 GO:0019899 GO:0032991 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0051020 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1

Protein Analysis

1187

Amino Acids

135.33

Weight (kDa)

6.29

Isoelectric Point (pI)

41.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	19 - 670	1.8e-156	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g	PF09334	49 - 188	5.9e-06	tRNA synthetases class I (M)
Anticodon_1	PF08264	728 - 877	4.5e-26	Anticodon-binding domain of tRNA ligase
DUF5915	PF19302	897 - 1047	1.7e-23	Domain of unknown function (DUF5915)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0009542)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G10320
fragaria_vesca	FvH4_4g11470
malus_domestica	MD04G1037200.v1.1 MD09G1065200.v1.1
prunus_persica	Prupe.1G186600_v2.0.a1 Prupe.1G186600_v2.0.a1
pyrus_communis	pycom04g03030 pycom111g05510
rosa_chinensis	RchiOBHm_Chr4g0408181
rosa_laevigata	RLG00000008666 RLG00000035346
rosa_multiflora	Rmu_ssc0000039.1_g000001
rosa_roxburghii	Rroxscaffold_1G00020510 Rroxscaffold_5G00352680
rosa_rugosa	Rorug04G0083900 Rorug04G0084000 Rorug05G0328300
rosa_samantha	Rh4AG148700 Rh4BG145500 Rh4CG155200 Rh4DG142000
rosa_wichuraiana	Rw4G012140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	447
AarI	CACCTGC	1 cut(s)	3029
Acc36I	ACCTGC	2 cut(s)	1855, 3029
AccB1I	GGYRCC	1 cut(s)	188
AccB7I	CCANNNNNTGG	1 cut(s)	641
AccBSI	CCGCTC	1 cut(s)	568
AccI	GTMKAC	1 cut(s)	3120
AccII	CGCG	7 cut(s)	211, 244, 313, 318, 2004, 2006, 3338
AccIII	TCCGGA	1 cut(s)	3016
AclWI	GGATC	9 cut(s)	347, 434, 605, 847, 873, 886, 1794, 2198, 3420
AcoI	YGGCCR	2 cut(s)	172, 229
AcsI	RAATTY	6 cut(s)	463, 727, 862, 1765, 3374, 3478
AcuI	CTGAAG	5 cut(s)	636, 1174, 1797, 2529, 3446
AcyI	GRCGYC	1 cut(s)	563
AdeI	CACNNNGTG	1 cut(s)	716
AfaI	GTAC	9 cut(s)	125, 350, 758, 831, 1051, 1881, 1971, 2072, 2341
AfiI	CCNNNNNNNGG	4 cut(s)	170, 641, 1079, 3262
AflIII	ACRYGT	1 cut(s)	3511
AjiI	CACGTC	1 cut(s)	238
AjnI	CCWGG	3 cut(s)	671, 1759, 3314
AjuI	GAANNNNNNNTTGG	4 cut(s)	1314, 1346, 1465, 1497
AluBI	AGCT	8 cut(s)	301, 663, 800, 1555, 1961, 2502, 2683, 2908
AluI	AGCT	8 cut(s)	301, 663, 800, 1555, 1961, 2502, 2683, 2908
Alw21I	GWGCWC	2 cut(s)	1072, 1639
Alw26I	GTCTC	4 cut(s)	83, 1127, 2101, 2590
AlwI	GGATC	9 cut(s)	347, 434, 605, 847, 873, 886, 1794, 2198, 3420
Ama87I	CYCGRG	2 cut(s)	119, 3048
Aor13HI	TCCGGA	1 cut(s)	3016
ApeKI	GCWGC	1 cut(s)	2499
ApoI	RAATTY	6 cut(s)	463, 727, 862, 1765, 3374, 3478
Asp700I	GAANNNNTTC	1 cut(s)	2487
AspLEI	GCGC	6 cut(s)	213, 246, 315, 2004, 2006, 2368
AspS9I	GGNCC	6 cut(s)	69, 112, 140, 2335, 2413, 2440
AsuC2I	CCSGG	1 cut(s)	1349
AsuHPI	GGTGA	7 cut(s)	97, 656, 1094, 1583, 2357, 2999, 3010
AvaI	CYCGRG	2 cut(s)	119, 3048
AvaII	GGWCC	3 cut(s)	69, 112, 2440
AxyI	CCTNAGG	2 cut(s)	883, 2624
BaeGI	GKGCMC	2 cut(s)	959, 1490
BamHI	GGATCC	1 cut(s)	878
BanI	GGYRCC	1 cut(s)	188
BanII	GRGCYC	1 cut(s)	2772
BauI	CACGAG	1 cut(s)	1794
BbsI	GAAGAC	2 cut(s)	121, 1092
Bbv12I	GWGCWC	2 cut(s)	1072, 1639
BbvCI	CCTCAGC	1 cut(s)	1887
BbvI	GCAGC	1 cut(s)	2511
BceAI	ACGGC	6 cut(s)	154, 187, 283, 1313, 2322, 3473
BcgI	CGANNNNNNTGC	4 cut(s)	891, 925, 2954, 2988
BciT130I	CCWGG	3 cut(s)	673, 1761, 3316
BclI	TGATCA	1 cut(s)	2146
BcnI	CCSGG	1 cut(s)	1349
BcoDI	GTCTC	4 cut(s)	83, 1127, 2101, 2590
BfaI	CTAG	6 cut(s)	699, 1626, 2553, 2798, 3095, 3311
BfoI	RGCGCY	1 cut(s)	2369
BfuAI	ACCTGC	2 cut(s)	1855, 3029
BglI	GCCNNNNNGGC	1 cut(s)	252
BglII	AGATCT	1 cut(s)	1301
BisI	GCNGC	5 cut(s)	247, 316, 566, 2500, 2795
BlsI	GCNGC	5 cut(s)	248, 317, 567, 2501, 2796
BmcAI	AGTACT	1 cut(s)	758
Bme1390I	CCNGG	4 cut(s)	673, 1349, 1761, 3316
Bme18I	GGWCC	3 cut(s)	69, 112, 2440
BmeT110I	CYCGRG	2 cut(s)	119, 3048
BmgBI	CACGTC	1 cut(s)	238
BmgT120I	GGNCC	6 cut(s)	69, 112, 140, 2335, 2413, 2440
BmrFI	CCNGG	4 cut(s)	673, 1349, 1761, 3316
BmrI	ACTGGG	2 cut(s)	426, 1408
BmsI	GCATC	7 cut(s)	346, 1154, 1954, 2962, 3025, 3193, 3228
BmuI	ACTGGG	2 cut(s)	426, 1408
BpiI	GAAGAC	2 cut(s)	121, 1092
BpmI	CTGGAG	2 cut(s)	2720, 2895
Bpu10I	CCTNAGC	1 cut(s)	1887
BpuEI	CTTGAG	1 cut(s)	2132
BpuMI	CCSGG	1 cut(s)	1349
Bsa29I	ATCGAT	2 cut(s)	429, 1947
BsaAI	YACGTR	1 cut(s)	2762
BsaHI	GRCGYC	1 cut(s)	563
BsaI	GGTCTC	1 cut(s)	1127
BsaJI	CCNNGG	5 cut(s)	108, 265, 686, 2066, 3277
BsaWI	WCCGGW	1 cut(s)	3016
BsaXI	ACNNNNNCTCC	4 cut(s)	464, 494, 667, 697
Bsc4I	CCNNNNNNNGG	4 cut(s)	170, 641, 1079, 3262
Bse118I	RCCGGY	4 cut(s)	155, 185, 227, 1593
Bse1I	ACTGG	7 cut(s)	71, 102, 217, 421, 1057, 1403, 2185
Bse21I	CCTNAGG	2 cut(s)	883, 2624
Bse3DI	GCAATG	7 cut(s)	1291, 1876, 2415, 2494, 2844, 2896, 3218
BseAI	TCCGGA	1 cut(s)	3016
BseBI	CCWGG	3 cut(s)	673, 1761, 3316
BseCI	ATCGAT	2 cut(s)	429, 1947
BseDI	CCNNGG	5 cut(s)	108, 265, 686, 2066, 3277
BseLI	CCNNNNNNNGG	4 cut(s)	170, 641, 1079, 3262
BseMI	GCAATG	7 cut(s)	1291, 1876, 2415, 2494, 2844, 2896, 3218
BseMII	CTCAG	8 cut(s)	509, 897, 1878, 2211, 2219, 2427, 2638, 3384
BseNI	ACTGG	7 cut(s)	71, 102, 217, 421, 1057, 1403, 2185
BsePI	GCGCGC	1 cut(s)	2002
BseRI	GAGGAG	2 cut(s)	62, 1482
BseSI	GKGCMC	2 cut(s)	959, 1490
BseXI	GCAGC	1 cut(s)	2511
BseYI	CCCAGC	1 cut(s)	255
BsgI	GTGCAG	1 cut(s)	2784
Bsh1236I	CGCG	7 cut(s)	211, 244, 313, 318, 2004, 2006, 3338
BshNI	GGYRCC	1 cut(s)	188
BshVI	ATCGAT	2 cut(s)	429, 1947
BsiHKAI	GWGCWC	2 cut(s)	1072, 1639
BsiHKCI	CYCGRG	2 cut(s)	119, 3048
BsiSI	CCGG	7 cut(s)	156, 186, 228, 364, 1348, 1594, 3017
BslFI	GGGAC	2 cut(s)	272, 2123
BslI	CCNNNNNNNGG	4 cut(s)	170, 641, 1079, 3262
BsmAI	GTCTC	4 cut(s)	83, 1127, 2101, 2590
BsmFI	GGGAC	2 cut(s)	272, 2123
BsmI	GAATGC	4 cut(s)	1279, 2104, 2487, 2861
Bso31I	GGTCTC	1 cut(s)	1127
BsoBI	CYCGRG	2 cut(s)	119, 3048
Bsp1286I	GDGCHC	5 cut(s)	959, 1072, 1490, 1639, 2772
Bsp13I	TCCGGA	1 cut(s)	3016
Bsp19I	CCATGG	2 cut(s)	2066, 3277
BspCNI	CTCAG	8 cut(s)	508, 896, 1879, 2210, 2218, 2428, 2637, 3383
BspDI	ATCGAT	2 cut(s)	429, 1947
BspEI	TCCGGA	1 cut(s)	3016
BspFNI	CGCG	7 cut(s)	211, 244, 313, 318, 2004, 2006, 3338
BspMI	ACCTGC	2 cut(s)	1855, 3029
BspPI	GGATC	9 cut(s)	347, 434, 605, 847, 873, 886, 1794, 2198, 3420
BspQI	GCTCTTC	2 cut(s)	807, 1554
BspT107I	GGYRCC	1 cut(s)	188
BspTNI	GGTCTC	1 cut(s)	1127
BsrBI	CCGCTC	1 cut(s)	568
BsrDI	GCAATG	7 cut(s)	1291, 1876, 2415, 2494, 2844, 2896, 3218
BsrFI	RCCGGY	4 cut(s)	155, 185, 227, 1593
BsrI	ACTGG	7 cut(s)	71, 102, 217, 421, 1057, 1403, 2185
BssAI	RCCGGY	4 cut(s)	155, 185, 227, 1593
BssECI	CCNNGG	5 cut(s)	108, 265, 686, 2066, 3277
BssHII	GCGCGC	1 cut(s)	2002
BssNI	GRCGYC	1 cut(s)	563
BssSI	CACGAG	1 cut(s)	1794
BssT1I	CCWWGG	3 cut(s)	108, 2066, 3277
Bst2BI	CACGAG	1 cut(s)	1794
Bst2UI	CCWGG	3 cut(s)	673, 1761, 3316
Bst4CI	ACNGT	6 cut(s)	1046, 1067, 2013, 2260, 3463, 3526
Bst6I	CTCTTC	4 cut(s)	699, 807, 1554, 3261
BstACI	GRCGYC	1 cut(s)	563
BstAPI	GCANNNNNTGC	3 cut(s)	555, 3044, 3350
BstBAI	YACGTR	1 cut(s)	2762
BstC8I	GCNNGC	7 cut(s)	187, 361, 661, 2004, 2792, 3321, 3486
BstDEI	CTNAG	9 cut(s)	495, 883, 1887, 2197, 2205, 2436, 2624, 3325, 3370
BstDSI	CCRYGG	4 cut(s)	265, 686, 2066, 3277
BstEII	GGTNACC	1 cut(s)	2243
BstFNI	CGCG	7 cut(s)	211, 244, 313, 318, 2004, 2006, 3338
BstH2I	RGCGCY	1 cut(s)	2369
BstHHI	GCGC	6 cut(s)	213, 246, 315, 2004, 2006, 2368
BstMAI	GTCTC	4 cut(s)	83, 1127, 2101, 2590
BstMWI	GCNNNNNNNGC	8 cut(s)	252, 261, 555, 1561, 2787, 3044, 3320, 3350
BstNI	CCWGG	3 cut(s)	673, 1761, 3316
BstNSI	RCATGY	2 cut(s)	3251, 3515
BstPI	GGTNACC	1 cut(s)	2243
BstSCI	CCNGG	4 cut(s)	671, 1347, 1759, 3314
BstSLI	GKGCMC	2 cut(s)	959, 1490
BstUI	CGCG	7 cut(s)	211, 244, 313, 318, 2004, 2006, 3338
BstV1I	GCAGC	1 cut(s)	2511
BstV2I	GAAGAC	2 cut(s)	121, 1092
BstX2I	RGATCY	3 cut(s)	839, 878, 1301
BstYI	RGATCY	3 cut(s)	839, 878, 1301
Bsu15I	ATCGAT	2 cut(s)	429, 1947
Bsu36I	CCTNAGG	2 cut(s)	883, 2624
BsuTUI	ATCGAT	2 cut(s)	429, 1947
BtgI	CCRYGG	4 cut(s)	265, 686, 2066, 3277
BtrI	CACGTC	1 cut(s)	238
BtsI	GCAGTG	2 cut(s)	1242, 2952
BtsIMutI	CAGTG	7 cut(s)	414, 1051, 1063, 1242, 2192, 2433, 2952
BveI	ACCTGC	2 cut(s)	1855, 3029
Cac8I	GCNNGC	7 cut(s)	187, 361, 661, 2004, 2792, 3321, 3486
CfoI	GCGC	6 cut(s)	213, 246, 315, 2004, 2006, 2368
Cfr10I	RCCGGY	4 cut(s)	155, 185, 227, 1593
Cfr13I	GGNCC	6 cut(s)	69, 112, 140, 2335, 2413, 2440
ClaI	ATCGAT	2 cut(s)	429, 1947
CseI	GACGC	3 cut(s)	571, 2930, 3350
Csp6I	GTAC	9 cut(s)	124, 349, 757, 830, 1050, 1880, 1970, 2071, 2340
CspCI	CAANNNNNGTGG	2 cut(s)	648, 683
CviQI	GTAC	9 cut(s)	124, 349, 757, 830, 1050, 1880, 1970, 2071, 2340
DdeI	CTNAG	9 cut(s)	495, 883, 1887, 2197, 2205, 2436, 2624, 3325, 3370
DraI	TTTAAA	1 cut(s)	988
DraIII	CACNNNGTG	1 cut(s)	716
EaeI	YGGCCR	2 cut(s)	172, 229
Eam1104I	CTCTTC	4 cut(s)	699, 807, 1554, 3261
EarI	CTCTTC	4 cut(s)	699, 807, 1554, 3261
EciI	GGCGGA	1 cut(s)	903
Eco130I	CCWWGG	3 cut(s)	108, 2066, 3277
Eco147I	AGGCCT	2 cut(s)	513, 3319
Eco24I	GRGCYC	1 cut(s)	2772
Eco31I	GGTCTC	1 cut(s)	1127
Eco32I	GATATC	1 cut(s)	2671
Eco47I	GGWCC	3 cut(s)	69, 112, 2440
Eco57I	CTGAAG	5 cut(s)	636, 1174, 1797, 2529, 3446
Eco81I	CCTNAGG	2 cut(s)	883, 2624
Eco88I	CYCGRG	2 cut(s)	119, 3048
Eco91I	GGTNACC	1 cut(s)	2243
EcoO109I	RGGNCCY	2 cut(s)	112, 2440
EcoO65I	GGTNACC	1 cut(s)	2243
EcoRI	GAATTC	1 cut(s)	3374
EcoRII	CCWGG	3 cut(s)	671, 1759, 3314
EcoRV	GATATC	1 cut(s)	2671
EcoT14I	CCWWGG	3 cut(s)	108, 2066, 3277
EcoT22I	ATGCAT	2 cut(s)	1281, 2080
EcoT38I	GRGCYC	1 cut(s)	2772
ErhI	CCWWGG	3 cut(s)	108, 2066, 3277
FalI	AAGNNNNNCTT	2 cut(s)	1239, 1271
FaqI	GGGAC	2 cut(s)	272, 2123
FauI	CCCGC	1 cut(s)	3493
FauNDI	CATATG	1 cut(s)	2808
FbaI	TGATCA	1 cut(s)	2146
FblI	GTMKAC	1 cut(s)	3120
Fnu4HI	GCNGC	5 cut(s)	247, 316, 566, 2500, 2795
FriOI	GRGCYC	1 cut(s)	2772
Fsp4HI	GCNGC	5 cut(s)	247, 316, 566, 2500, 2795
FspBI	CTAG	6 cut(s)	699, 1626, 2553, 2798, 3095, 3311
GlaI	GCGC	6 cut(s)	212, 245, 314, 2003, 2005, 2367
GluI	GCNGC	5 cut(s)	247, 316, 566, 2500, 2795
GsaI	CCCAGC	1 cut(s)	259
GsuI	CTGGAG	2 cut(s)	2720, 2895
HaeII	RGCGCY	1 cut(s)	2369
HapII	CCGG	7 cut(s)	156, 186, 228, 364, 1348, 1594, 3017
HgaI	GACGC	3 cut(s)	571, 2930, 3350
HhaI	GCGC	6 cut(s)	213, 246, 315, 2004, 2006, 2368
Hin1I	GRCGYC	1 cut(s)	563
Hin6I	GCGC	6 cut(s)	211, 244, 313, 2002, 2004, 2366
HinP1I	GCGC	6 cut(s)	211, 244, 313, 2002, 2004, 2366
HincII	GTYRAC	1 cut(s)	907
HindII	GTYRAC	1 cut(s)	907
HindIII	AAGCTT	1 cut(s)	661
HpaII	CCGG	7 cut(s)	156, 186, 228, 364, 1348, 1594, 3017
HphI	GGTGA	7 cut(s)	97, 656, 1094, 1583, 2357, 2999, 3010
Hpy166II	GTNNAC	4 cut(s)	690, 907, 1063, 3121
Hpy8I	GTNNAC	4 cut(s)	690, 907, 1063, 3121
Hpy99I	CGWCG	3 cut(s)	140, 281, 1659
HpyCH4III	ACNGT	6 cut(s)	1046, 1067, 2013, 2260, 3463, 3526
HpyCH4IV	ACGT	9 cut(s)	126, 237, 1657, 2017, 2055, 2533, 2712, 2761, 3013
HpyF10VI	GCNNNNNNNGC	8 cut(s)	252, 261, 555, 1561, 2787, 3044, 3320, 3350
HpyF3I	CTNAG	9 cut(s)	495, 883, 1887, 2197, 2205, 2436, 2624, 3325, 3370
HpySE526I	ACGT	9 cut(s)	126, 237, 1657, 2017, 2055, 2533, 2712, 2761, 3013
Hsp92I	GRCGYC	1 cut(s)	563
HspAI	GCGC	6 cut(s)	211, 244, 313, 2002, 2004, 2366
Kpn2I	TCCGGA	1 cut(s)	3016
KroI	GCCGGC	1 cut(s)	185
KroNI	GCCGGC	1 cut(s)	187
Ksp22I	TGATCA	1 cut(s)	2146
LguI	GCTCTTC	2 cut(s)	807, 1554
LmnI	GCTCC	4 cut(s)	366, 1075, 1354, 1958
Lsp1109I	GCAGC	1 cut(s)	2511
LweI	GCATC	7 cut(s)	346, 1154, 1954, 2962, 3025, 3193, 3228
MaeI	CTAG	6 cut(s)	699, 1626, 2553, 2798, 3095, 3311
MaeII	ACGT	9 cut(s)	126, 237, 1657, 2017, 2055, 2533, 2712, 2761, 3013
MaeIII	GTNAC	6 cut(s)	238, 373, 1033, 2013, 2243, 2881
MbiI	CCGCTC	1 cut(s)	568
MfeI	CAATTG	3 cut(s)	1435, 1935, 3345
MflI	RGATCY	3 cut(s)	839, 878, 1301
MhlI	GDGCHC	5 cut(s)	959, 1072, 1490, 1639, 2772
MlyI	GAGTC	7 cut(s)	85, 685, 902, 1786, 2218, 3032, 3122
MmeI	TCCRAC	1 cut(s)	2493
Mph1103I	ATGCAT	2 cut(s)	1281, 2080
MroI	TCCGGA	1 cut(s)	3016
MroNI	GCCGGC	1 cut(s)	185
MroXI	GAANNNNTTC	1 cut(s)	2487
MslI	CAYNNNNRTG	1 cut(s)	1814
MspI	CCGG	7 cut(s)	156, 186, 228, 364, 1348, 1594, 3017
MspR9I	CCNGG	4 cut(s)	673, 1349, 1761, 3316
MunI	CAATTG	3 cut(s)	1435, 1935, 3345
Mva1269I	GAATGC	4 cut(s)	1279, 2104, 2487, 2861
MvaI	CCWGG	3 cut(s)	673, 1761, 3316
MvnI	CGCG	7 cut(s)	211, 244, 313, 318, 2004, 2006, 3338
MwoI	GCNNNNNNNGC	8 cut(s)	252, 261, 555, 1561, 2787, 3044, 3320, 3350
NaeI	GCCGGC	1 cut(s)	187
NciI	CCSGG	1 cut(s)	1349
NcoI	CCATGG	2 cut(s)	2066, 3277
NdeI	CATATG	1 cut(s)	2808
NgoMIV	GCCGGC	1 cut(s)	185
NmuCI	GTSAC	1 cut(s)	238
NsiI	ATGCAT	2 cut(s)	1281, 2080
NspI	RCATGY	2 cut(s)	3251, 3515
PaeR7I	CTCGAG	1 cut(s)	3048
PaqCI	CACCTGC	1 cut(s)	3029
PauI	GCGCGC	1 cut(s)	2002
PceI	AGGCCT	2 cut(s)	513, 3319
PciI	ACATGT	1 cut(s)	3511
PciSI	GCTCTTC	2 cut(s)	807, 1554
PcsI	WCGNNNNNNNCGW	2 cut(s)	132, 273
PctI	GAATGC	4 cut(s)	1279, 2104, 2487, 2861
PdiI	GCCGGC	1 cut(s)	187
PdmI	GAANNNNTTC	1 cut(s)	2487
PfeI	GAWTC	7 cut(s)	55, 647, 785, 848, 1428, 1541, 1858
PflMI	CCANNNNNTGG	1 cut(s)	641
PfoI	TCCNGGA	1 cut(s)	1759
PkrI	GCNGC	5 cut(s)	248, 317, 567, 2501, 2796
PleI	GAGTC	7 cut(s)	85, 685, 902, 1786, 2217, 3031, 3122
PpsI	GAGTC	7 cut(s)	85, 685, 902, 1786, 2217, 3031, 3122
Ppu21I	YACGTR	1 cut(s)	2762
PpuMI	RGGWCCY	2 cut(s)	112, 2440
PscI	ACATGT	1 cut(s)	3511
PsiI	TTATAA	1 cut(s)	447
Psp5II	RGGWCCY	2 cut(s)	112, 2440
Psp6I	CCWGG	3 cut(s)	671, 1759, 3314
PspEI	GGTNACC	1 cut(s)	2243
PspFI	CCCAGC	1 cut(s)	255
PspGI	CCWGG	3 cut(s)	671, 1759, 3314
PspPI	GGNCC	6 cut(s)	69, 112, 140, 2335, 2413, 2440
PspPPI	RGGWCCY	2 cut(s)	112, 2440
PsuI	RGATCY	3 cut(s)	839, 878, 1301
PteI	GCGCGC	1 cut(s)	2002
RsaI	GTAC	9 cut(s)	125, 350, 758, 831, 1051, 1881, 1971, 2072, 2341
RsaNI	GTAC	9 cut(s)	124, 349, 757, 830, 1050, 1880, 1970, 2071, 2340
RseI	CAYNNNNRTG	1 cut(s)	1814
SapI	GCTCTTC	2 cut(s)	807, 1554
SatI	GCNGC	5 cut(s)	247, 316, 566, 2500, 2795
Sau96I	GGNCC	6 cut(s)	69, 112, 140, 2335, 2413, 2440
ScaI	AGTACT	1 cut(s)	758
SchI	GAGTC	7 cut(s)	85, 685, 902, 1786, 2218, 3032, 3122
ScrFI	CCNGG	4 cut(s)	673, 1349, 1761, 3316
SduI	GDGCHC	5 cut(s)	959, 1072, 1490, 1639, 2772
SfaNI	GCATC	7 cut(s)	346, 1154, 1954, 2962, 3025, 3193, 3228
Sfr274I	CTCGAG	1 cut(s)	3048
SinI	GGWCC	3 cut(s)	69, 112, 2440
SlaI	CTCGAG	1 cut(s)	3048
SmiMI	CAYNNNNRTG	1 cut(s)	1814
SmlI	CTYRAG	2 cut(s)	2111, 3048
SmoI	CTYRAG	2 cut(s)	2111, 3048
SseBI	AGGCCT	2 cut(s)	513, 3319
SspI	AATATT	1 cut(s)	2587
SspMI	CTAG	6 cut(s)	699, 1626, 2553, 2798, 3095, 3311
StuI	AGGCCT	2 cut(s)	513, 3319
StyD4I	CCNGG	4 cut(s)	671, 1347, 1759, 3314
StyI	CCWWGG	3 cut(s)	108, 2066, 3277
TaaI	ACNGT	6 cut(s)	1046, 1067, 2013, 2260, 3463, 3526
TaiI	ACGT	9 cut(s)	129, 240, 1660, 2020, 2058, 2536, 2715, 2764, 3016
TatI	WGTACW	3 cut(s)	348, 756, 1879
TauI	GCSGC	4 cut(s)	249, 318, 568, 2797
TfiI	GAWTC	7 cut(s)	55, 647, 785, 848, 1428, 1541, 1858
TscAI	CASTG	7 cut(s)	421, 1051, 1070, 1242, 2192, 2440, 2959
TseFI	GTSAC	1 cut(s)	238
TseI	GCWGC	1 cut(s)	2499
Tsp45I	GTSAC	1 cut(s)	238
TspDTI	ATGAA	8 cut(s)	519, 936, 981, 2252, 2480, 2544, 2633, 3285
TspGWI	ACGGA	4 cut(s)	675, 1199, 2216, 3465
TspRI	CASTG	7 cut(s)	421, 1051, 1070, 1242, 2192, 2440, 2959
Van91I	CCANNNNNTGG	1 cut(s)	641
VpaK11BI	GGWCC	3 cut(s)	69, 112, 2440
XapI	RAATTY	6 cut(s)	463, 727, 862, 1765, 3374, 3478
XbaI	TCTAGA	1 cut(s)	3094
XceI	RCATGY	2 cut(s)	3251, 3515
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3284
XhoI	CTCGAG	1 cut(s)	3048
XmiI	GTMKAC	1 cut(s)	3120
XmnI	GAANNNNTTC	1 cut(s)	2487
XspI	CTAG	6 cut(s)	699, 1626, 2553, 2798, 3095, 3311
ZrmI	AGTACT	1 cut(s)	758
Zsp2I	ATGCAT	2 cut(s)	1281, 2080

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.