Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0009542

Physical Location & Seq

Genomic Coordinates Forward (+)

4145289 .. 4157969

Gene Structure 12681 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1037200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3672 bp

ATGGAGGAGGTGTGCGAGGGCAAGGACTTCTCCTTCCCCAAGCAAGAGGAGACTATCCTCAAGCTCTGGTCTGAAATCAAGGCCTTCGAAACCCAATTGGCGCGCACAGAGGGCCAGCCCGAGTACGTCTTCTATGACGGTCCTCCATTCGCCACCGGACTCCCACACTACGGCCACATACTGGCCGGCACCATCAAAGACGTCATCACCCGATTCTTCTCCATGACGGGCCACCACGTCATCCGCCGCTTCGGCTGGGACTGCCACGGTCTGCCGGTGGAGAACGAGATCGATCGGATGCTCGGGATCAAGCGTCGGGACGATGTGCTGAAGATGGGGATTGATAAGTACAATGAAGCCTGCAGGAGCATTGTTACTCGGTACGTGGGGGAGTGGGAGAAGGTTATTTCGCGGACGGGGCGGTGGATTGATTTCAATAACGATTACAAGACGATGGATTTGAAGTTCATGGAGAGCGTTTGGTGGGTCTTTGGCCAGTTATTTGAGAAGGGTCTTGTTTATAAAGGCTTCAAGGTCATGCCATACAGCACTGGTTGCAAGACACCGCTTTCCAATTTTGAGGCTGGTCAAGAGTACAGGGATGTACCTGATCCTGAAATTATGGTGACTTTTCCAATTGTTGGAGATCCGCAAAAGGCAAATTTTGTAGCTTGGACAACTACACCATGGACCCTCCCGAGCAATTTGGCCCTTTGTGTCAATGCAAATTTTACTTATGTGAAGGTCCGCAACAAGTACTCTGGAAAAGTTTATGTTGTTGCTGAATCTCGGTTGTCAGCTCTTCCTAATGAGAAACCAAAGGAAAATGTAGCACATGGATCTGTTGGTGATTCAAAAAAGCTAAATTCAAAGGCCAAAGGATCCTCAGGTGGGAAAAAGGAAACCGTGGACAGTTCATATGAAGTATTGGAGAAATTCTCTGGTTCTTCGTTAGAGGGAAAGAAGTATGAACCGCTGTTTGACTACTTTACGGAGTTCTCTGATGTGGCATTTAGAGTTGTTGTGGACAACTATGTTACCAATGACAGTGGTACTGGTATTGTGCATTGTGCTCCTGCATTTGGTGAAGATGATTATCGTGTTTGCCTTGAAAATCAAGTAATCAACAAGGGAGAGAACTTGATTGTAGCTGTTGATGATGATGGATGCTTTACCGAAAGAATTACCGACTTCAGCAAGTGCTATGTCAAGGATGCAGATAAAGATATTATTGAAGCAGTGAAGGCAAAAGGAAGGCTAGTGAAGTCTGGAACGGTTACTCATTCTTATCCCTTTTGCTGGAGATCTAAAACTCCGCTTATTTACAGAGCTGTTCCAAGCTGGTTTATCCGAGTGGAGCTGTTGAAAGAAAAATTGTTAGAAAACAACAAGCAGACTTACTGGGTTCCTGATTTTGTCAAGGAAAAGCGCTTTCACAATTGGCTGGAGAATGCAAGAGATTGGGCTGTTAGTCGAAGTAGATTTTGGGGCACTCCACTTCCAGTCTGGATTAGTGAGGATGGTGAAGAAATTGTTGTAATGGATTCTATTCAGAAGCTTGAAGAGCTTTCTGGTGTTAAGGTTTTCGATCTGCACCGCCACAATATTGATCACATTACTATTCCCTCAAGCCGAGGTCCCGAATATGGTGTTCTTCGACGTATAGATGATGTGTTTGATTGTTGGTTTGAGAGTGGGTCCATGCCTTATGCTTATATCCACTATCCTTTTGAGAATGTTGAACATTTTGAGGAAAATTTCCCTGGCCATTTTGTGGCTGAGGGGCTAGATCAGACTCGTGGATGGTTCTACACTCTTATGGTGTTATCTACGGCGTTATTTGGGAAACCTGCTTTTAGGAACCTCATTTGCAATGGACTTGTCCTTGCTGAGGATGGGAAAAAAATGAGTAAAAGTTTGAAAAATTATCCTTCACCAATGGAAGTTATCGATGACTATGGGGCCGATGCACTAAGACTATACCTCATTAACTCTCCTGTTGTGCGTGCGGAGCCGTTACGTTTCAAGAAGGAAGGAGTTTTTGGTGTTGTCAAGGATGTATTTCTTCCTTGGTACAATGCATATCGGTTCCTTGTTCAGAATGCAAAGAGACTTGAAGTTGAAGGGTTTTCCCCTTTCCTCCCTGTTGATCTAGCCACTGTACAGAAGTCGTCTAATGTGCTTGACCAGTGGATCAATTCAGCCACACAGAGTCTTGTTTATTTTGTTCAGCAAGAGATGAATGGTTATCGACTTTATACGGTGGTTCCATATCTTTTGAAGTTTCTTGACAACTTAACAAATATTTATGTGCGGTTCAATCGGAAGAGGCTAAAGGGTCGAACTGGTGAAGAGGATTGCAGAATGGCACTTTCAACTCTCTACAATGTCCTCTTGGTGTCATGTAAAGTAATGGCACCATTAACACCATTCTTCACGGAGGTCCTTTATCAAAATATGCGGAAAGCTTTGAATGAGTCAGAGGAAAGCATTCATTTTTGCAGTTTTCCTGAAGGAGAAGGAAAGACAGACGAGCGTATAGAGCAAAGTGTTACAAGAATGATGACAACAATTGATCTTGCCCGTAATATTCGTGAGCGTCACAACAAGCCTCTCAAAACACCCCTGAGGGAGATGGTTATTGTTCATCCTGATGCAGACTTTCTTGATGATATTGGTGGAAAGCTAAGAGAGTATGTATTGGAGGAACTTAATGTAAGATCTCTTGTCCCATGTAATGATACACTGAAGTATGCTTCGTTACGAGCAGAGCCTGATTTTAGCGTATTAGGTAAGCGACTTGGAAAATCTATGGGAGTTGTTGCCAAAGAAGTCAAGGCCATGTCTCAAGAAAGCATTTTAGCATTTGAGAAAGCTGGAGAAGTTACTCTTTCTGGTCATTGTTTAAAGCTGGGTGATATTAAGGTTGTTAGGGATTTCAAACGTCCTAATGGAACGACAGAAAAGGAGATTGATGCTACAGGGGACGGTGATGTTTTGGTGATTTTGGATTTACGTCCAGATGAGTCATTGTTTGAGGCCGGTATTGCTCGTGAGATTGTAAACAGAATTCAGAAGTTACGGAAGAAAGCTGCCCTTGAACCTACTGACATGATGGAGGCATACTTTGAATCGTTGGATCAAGATAAATCAGTCTCACAACGGGTTTTGCACTCTCAGGAACAATACATTAGGGATACCATTGGTTTGCCATTGCTTCTGTCTAGTGTGATGCCATCAGATGCTGTCGTTGTTGCGGAGGAGAGTTTCCATGGGATATCTGGCATGTCATTTGTTATCAGTTTGACCCGACCTGTGCCAGTGCTTGACTCAGATGCAATTCTTTCTCTATATTCAGGAAACACAGAATTTGCACGGGCTCTGCAAACGTACTTGTTATCAAGGGATCATGCAAATTTGAAGTCCGAGTTTCAACAAGGAAAGGGAAAGATAAATGTTGATTGCATTGAGAACACTCCTGCTGTGGAACTGGTGTTGGGGGATCATGTATTCTTGACCGTCGGGGACTTCATTTCTAGAACAAAATCTCAATGTAAACGACGCATACTGAAAGCAGCAAGAGATGGCCTAATTGTTGTTTCAAAAGGCCTTAATACAGAACTAAATACGATATTTTGGTTATTGAGAAGTGATGTGAGATTGAATATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004822 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006428 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0017101 GO:0019538 GO:0019752 GO:0019899 GO:0032991 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0051020 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1

Protein Analysis

1224

Amino Acids

139.31

Weight (kDa)

6.02

Isoelectric Point (pI)

39.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	19 - 671	1e-157	tRNA synthetases class I (I, L, M and V)
Anticodon_1	PF08264	729 - 878	3e-26	Anticodon-binding domain of tRNA ligase
DUF5915	PF19302	898 - 1064	3.7e-23	Domain of unknown function (DUF5915)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009542)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G10320
fragaria_vesca	FvH4_4g11470
malus_domestica	MD04G1037200.v1.1 MD09G1065200.v1.1
prunus_persica	Prupe.1G186600_v2.0.a1 Prupe.1G186600_v2.0.a1
pyrus_communis	pycom04g03030 pycom111g05510
rosa_chinensis	RchiOBHm_Chr4g0408181
rosa_laevigata	RLG00000008666 RLG00000035346
rosa_multiflora	Rmu_ssc0000039.1_g000001
rosa_roxburghii	Rroxscaffold_1G00020510 Rroxscaffold_5G00352680
rosa_rugosa	Rorug04G0083900 Rorug04G0084000 Rorug05G0328300
rosa_samantha	Rh4AG148700 Rh4BG145500 Rh4CG155200 Rh4DG142000
rosa_wichuraiana	Rw4G012140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	522
AatII	GACGTC	1 cut(s)	204
Acc36I	ACCTGC	1 cut(s)	1858
AccB1I	GGYRCC	2 cut(s)	188, 2416
AccB7I	CCANNNNNTGG	3 cut(s)	181, 641, 1774
AccII	CGCG	2 cut(s)	103, 412
AcoI	YGGCCR	4 cut(s)	172, 183, 493, 1765
AcsI	RAATTY	8 cut(s)	661, 727, 865, 935, 1756, 3069, 3368, 3415
AcuI	CTGAAG	4 cut(s)	350, 1177, 2532, 2768
AcyI	GRCGYC	1 cut(s)	201
AfeI	AGCGCT	1 cut(s)	1430
AfiI	CCNNNNNNNGG	9 cut(s)	170, 181, 641, 891, 1082, 1299, 1646, 1774, 1891
AjiI	CACGTC	1 cut(s)	238
AjnI	CCWGG	1 cut(s)	1762
AjuI	GAANNNNNNNTTGG	4 cut(s)	1468, 1500, 2025, 2057
AloI	GAACNNNNNNTCC	2 cut(s)	449, 481
Alw21I	GWGCWC	1 cut(s)	1075
Alw26I	GTCTC	4 cut(s)	44, 2104, 2850, 3160
AlwNI	CAGNNNCTG	2 cut(s)	2774, 3245
Ama87I	CYCGRG	3 cut(s)	119, 302, 697
Aor51HI	AGCGCT	1 cut(s)	1430
ApeKI	GCWGC	2 cut(s)	3092, 3575
ApoI	RAATTY	8 cut(s)	661, 727, 865, 935, 1756, 3069, 3368, 3415
ArsI	GACNNNNNNTTYG	4 cut(s)	442, 474, 3407, 3439
Asp700I	GAANNNNTTC	1 cut(s)	2490
AspLEI	GCGC	3 cut(s)	103, 105, 1431
AsuII	TTCGAA	1 cut(s)	87
AvaI	CYCGRG	3 cut(s)	119, 302, 697
AvaII	GGWCC	6 cut(s)	140, 690, 745, 1637, 1698, 2443
AxyI	CCTNAGG	2 cut(s)	886, 2627
BaeGI	GKGCMC	1 cut(s)	1493
BalI	TGGCCA	2 cut(s)	495, 1767
BamHI	GGATCC	1 cut(s)	881
BanI	GGYRCC	2 cut(s)	188, 2416
BanII	GRGCYC	1 cut(s)	3382
BauI	CACGAG	2 cut(s)	1797, 3051
BbsI	GAAGAC	1 cut(s)	121
Bbv12I	GWGCWC	1 cut(s)	1075
BbvCI	CCTCAGC	2 cut(s)	1779, 1890
BbvI	GCAGC	2 cut(s)	3079, 3587
BceAI	ACGGC	3 cut(s)	187, 1848, 1999
BciT130I	CCWGG	1 cut(s)	1764
BciVI	GTATCC	1 cut(s)	3190
BclI	TGATCA	1 cut(s)	1609
BcoDI	GTCTC	4 cut(s)	44, 2104, 2850, 3160
BfaI	CTAG	5 cut(s)	1259, 1787, 2153, 3225, 3537
BfmI	CTRYAG	2 cut(s)	361, 2979
BfoI	RGCGCY	1 cut(s)	1432
BfuAI	ACCTGC	1 cut(s)	1858
BfuI	GTATCC	1 cut(s)	3190
BglI	GCCNNNNNGGC	1 cut(s)	252
BglII	AGATCT	2 cut(s)	1304, 2720
BisI	GCNGC	3 cut(s)	247, 3093, 3576
BlsI	GCNGC	3 cut(s)	248, 3094, 3577
BmcAI	AGTACT	1 cut(s)	758
Bme1390I	CCNGG	1 cut(s)	1764
Bme18I	GGWCC	6 cut(s)	140, 690, 745, 1637, 1698, 2443
BmeT110I	CYCGRG	3 cut(s)	119, 302, 697
BmgBI	CACGTC	1 cut(s)	238
BmrFI	CCNGG	1 cut(s)	1764
BmrI	ACTGGG	1 cut(s)	1411
BmsI	GCATC	9 cut(s)	288, 1157, 1204, 1957, 2644, 2965, 3222, 3232, 3325
BmuI	ACTGGG	1 cut(s)	1411
BpiI	GAAGAC	1 cut(s)	121
BplI	GAGNNNNNCTC	2 cut(s)	923, 955
BpmI	CTGGAG	3 cut(s)	1321, 1466, 2898
Bpu10I	CCTNAGC	2 cut(s)	1779, 1890
Bpu14I	TTCGAA	1 cut(s)	87
BpuEI	CTTGAG	3 cut(s)	44, 1612, 2832
Bsa29I	ATCGAT	2 cut(s)	291, 1950
BsaAI	YACGTR	1 cut(s)	385
BsaBI	GATNNNNATC	1 cut(s)	1095
BsaHI	GRCGYC	1 cut(s)	201
BsaJI	CCNNGG	7 cut(s)	265, 686, 906, 1633, 1762, 2069, 3271
BsaWI	WCCGGW	1 cut(s)	155
BsaXI	ACNNNNNCTCC	1 cut(s)	26
Bsc4I	CCNNNNNNNGG	9 cut(s)	170, 181, 641, 891, 1082, 1299, 1646, 1774, 1891
Bse118I	RCCGGY	3 cut(s)	185, 274, 3041
Bse21I	CCTNAGG	2 cut(s)	886, 2627
Bse3DI	GCAATG	2 cut(s)	1879, 3212
Bse8I	GATNNNNATC	1 cut(s)	1095
BseBI	CCWGG	1 cut(s)	1764
BseCI	ATCGAT	2 cut(s)	291, 1950
BseDI	CCNNGG	7 cut(s)	265, 686, 906, 1633, 1762, 2069, 3271
BseJI	GATNNNNATC	1 cut(s)	1095
BseLI	CCNNNNNNNGG	9 cut(s)	170, 181, 641, 891, 1082, 1299, 1646, 1774, 1891
BseMI	GCAATG	2 cut(s)	1879, 3212
BseMII	CTCAG	6 cut(s)	900, 1770, 1881, 2618, 3191, 3345
BsePI	GCGCGC	1 cut(s)	101
BseRI	GAGGAG	3 cut(s)	20, 62, 3275
BseSI	GKGCMC	1 cut(s)	1493
BseXI	GCAGC	2 cut(s)	3079, 3587
BseYI	CCCAGC	2 cut(s)	255, 2911
BsgI	GTGCAG	1 cut(s)	1577
Bsh1236I	CGCG	2 cut(s)	103, 412
Bsh1285I	CGRYCG	1 cut(s)	295
BshNI	GGYRCC	2 cut(s)	188, 2416
BshVI	ATCGAT	2 cut(s)	291, 1950
BsiEI	CGRYCG	1 cut(s)	295
BsiHKAI	GWGCWC	1 cut(s)	1075
BsiHKCI	CYCGRG	3 cut(s)	119, 302, 697
BsiSI	CCGG	4 cut(s)	156, 186, 275, 3042
BslFI	GGGAC	6 cut(s)	272, 332, 1623, 2714, 2999, 3539
BslI	CCNNNNNNNGG	9 cut(s)	170, 181, 641, 891, 1082, 1299, 1646, 1774, 1891
BsmAI	GTCTC	4 cut(s)	44, 2104, 2850, 3160
BsmFI	GGGAC	6 cut(s)	272, 332, 1623, 2714, 2999, 3539
BsmI	GAATGC	3 cut(s)	1456, 2107, 2490
BsoBI	CYCGRG	3 cut(s)	119, 302, 697
Bsp119I	TTCGAA	1 cut(s)	87
Bsp1286I	GDGCHC	3 cut(s)	1075, 1493, 3382
Bsp1407I	TGTACA	1 cut(s)	2161
Bsp19I	CCATGG	2 cut(s)	686, 3271
BspCNI	CTCAG	6 cut(s)	899, 1771, 1882, 2619, 3190, 3344
BspDI	ATCGAT	2 cut(s)	291, 1950
BspFNI	CGCG	2 cut(s)	103, 412
BspMAI	CTGCAG	1 cut(s)	365
BspMI	ACCTGC	1 cut(s)	1858
BspQI	GCTCTTC	2 cut(s)	807, 1557
BspT104I	TTCGAA	1 cut(s)	87
BspT107I	GGYRCC	2 cut(s)	188, 2416
BsrDI	GCAATG	2 cut(s)	1879, 3212
BsrFI	RCCGGY	3 cut(s)	185, 274, 3041
BsrGI	TGTACA	1 cut(s)	2161
BssAI	RCCGGY	3 cut(s)	185, 274, 3041
BssECI	CCNNGG	7 cut(s)	265, 686, 906, 1633, 1762, 2069, 3271
BssHII	GCGCGC	1 cut(s)	101
BssNI	GRCGYC	1 cut(s)	201
BssSI	CACGAG	2 cut(s)	1797, 3051
BssT1I	CCWWGG	3 cut(s)	686, 2069, 3271
Bst2BI	CACGAG	2 cut(s)	1797, 3051
Bst2UI	CCWGG	1 cut(s)	1764
Bst6I	CTCTTC	4 cut(s)	807, 1557, 2321, 2346
BstACI	GRCGYC	1 cut(s)	201
BstAPI	GCANNNNNTGC	1 cut(s)	555
BstAUI	TGTACA	1 cut(s)	2161
BstBAI	YACGTR	1 cut(s)	385
BstBI	TTCGAA	1 cut(s)	87
BstC8I	GCNNGC	5 cut(s)	103, 116, 187, 361, 2007
BstDEI	CTNAG	8 cut(s)	886, 1779, 1890, 1973, 2627, 2687, 3177, 3331
BstDSI	CCRYGG	4 cut(s)	265, 686, 906, 3271
BstENI	CCTNNNNNAGG	1 cut(s)	1889
BstFNI	CGCG	2 cut(s)	103, 412
BstH2I	RGCGCY	1 cut(s)	1432
BstHHI	GCGC	3 cut(s)	103, 105, 1431
BstMAI	GTCTC	4 cut(s)	44, 2104, 2850, 3160
BstMCI	CGRYCG	1 cut(s)	295
BstNI	CCWGG	1 cut(s)	1764
BstNSI	RCATGY	1 cut(s)	3289
BstSCI	CCNGG	1 cut(s)	1762
BstSFI	CTRYAG	2 cut(s)	361, 2979
BstSLI	GKGCMC	1 cut(s)	1493
BstUI	CGCG	2 cut(s)	103, 412
BstV1I	GCAGC	2 cut(s)	3079, 3587
BstV2I	GAAGAC	1 cut(s)	121
BstX2I	RGATCY	5 cut(s)	646, 839, 881, 1304, 2720
BstYI	RGATCY	5 cut(s)	646, 839, 881, 1304, 2720
Bsu15I	ATCGAT	2 cut(s)	291, 1950
Bsu36I	CCTNAGG	2 cut(s)	886, 2627
BsuI	GTATCC	1 cut(s)	3190
BsuTUI	ATCGAT	2 cut(s)	291, 1950
BtgI	CCRYGG	4 cut(s)	265, 686, 906, 3271
BtrI	CACGTC	1 cut(s)	238
BtsI	GCAGTG	1 cut(s)	1245
BtsIMutI	CAGTG	7 cut(s)	549, 1054, 1245, 2157, 2195, 2744, 3327
BveI	ACCTGC	1 cut(s)	1858
Cac8I	GCNNGC	5 cut(s)	103, 116, 187, 361, 2007
CaiI	CAGNNNCTG	2 cut(s)	2774, 3245
CfoI	GCGC	3 cut(s)	103, 105, 1431
Cfr10I	RCCGGY	3 cut(s)	185, 274, 3041
ClaI	ATCGAT	2 cut(s)	291, 1950
CseI	GACGC	3 cut(s)	302, 2588, 3570
CspCI	CAANNNNNGTGG	2 cut(s)	1030, 1065
DdeI	CTNAG	8 cut(s)	886, 1779, 1890, 1973, 2627, 2687, 3177, 3331
DraI	TTTAAA	1 cut(s)	2907
EaeI	YGGCCR	4 cut(s)	172, 183, 493, 1765
Eam1104I	CTCTTC	4 cut(s)	807, 1557, 2321, 2346
EarI	CTCTTC	4 cut(s)	807, 1557, 2321, 2346
EciI	GGCGGA	1 cut(s)	233
Eco130I	CCWWGG	3 cut(s)	686, 2069, 3271
Eco147I	AGGCCT	2 cut(s)	83, 3609
Eco24I	GRGCYC	1 cut(s)	3382
Eco32I	GATATC	1 cut(s)	3279
Eco47I	GGWCC	6 cut(s)	140, 690, 745, 1637, 1698, 2443
Eco47III	AGCGCT	1 cut(s)	1430
Eco57I	CTGAAG	4 cut(s)	350, 1177, 2532, 2768
Eco81I	CCTNAGG	2 cut(s)	886, 2627
Eco88I	CYCGRG	3 cut(s)	119, 302, 697
EcoNI	CCTNNNNNAGG	1 cut(s)	1889
EcoO109I	RGGNCCY	2 cut(s)	1637, 2443
EcoRI	GAATTC	1 cut(s)	3069
EcoRII	CCWGG	1 cut(s)	1762
EcoRV	GATATC	1 cut(s)	3279
EcoT14I	CCWWGG	3 cut(s)	686, 2069, 3271
EcoT22I	ATGCAT	1 cut(s)	2083
EcoT38I	GRGCYC	1 cut(s)	3382
ErhI	CCWWGG	3 cut(s)	686, 2069, 3271
FalI	AAGNNNNNCTT	4 cut(s)	2874, 2906, 3081, 3113
FaqI	GGGAC	6 cut(s)	272, 332, 1623, 2714, 2999, 3539
FauNDI	CATATG	1 cut(s)	919
FbaI	TGATCA	1 cut(s)	1609
Fnu4HI	GCNGC	3 cut(s)	247, 3093, 3576
FriOI	GRGCYC	1 cut(s)	3382
Fsp4HI	GCNGC	3 cut(s)	247, 3093, 3576
FspBI	CTAG	5 cut(s)	1259, 1787, 2153, 3225, 3537
GlaI	GCGC	3 cut(s)	102, 104, 1430
GluI	GCNGC	3 cut(s)	247, 3093, 3576
GsaI	CCCAGC	2 cut(s)	259, 2915
GsuI	CTGGAG	3 cut(s)	1321, 1466, 2898
HaeII	RGCGCY	1 cut(s)	1432
HapII	CCGG	4 cut(s)	156, 186, 275, 3042
HgaI	GACGC	3 cut(s)	302, 2588, 3570
HhaI	GCGC	3 cut(s)	103, 105, 1431
Hin1I	GRCGYC	1 cut(s)	201
Hin6I	GCGC	3 cut(s)	101, 103, 1429
HinP1I	GCGC	3 cut(s)	101, 103, 1429
HindIII	AAGCTT	2 cut(s)	1556, 2466
HpaII	CCGG	4 cut(s)	156, 186, 275, 3042
Hpy166II	GTNNAC	4 cut(s)	910, 1027, 3064, 3557
Hpy8I	GTNNAC	4 cut(s)	910, 1027, 3064, 3557
Hpy99I	CGWCG	4 cut(s)	318, 1662, 3524, 3564
HpyCH4IV	ACGT	9 cut(s)	126, 201, 237, 384, 1660, 2020, 2944, 3016, 3389
HpyF3I	CTNAG	8 cut(s)	886, 1779, 1890, 1973, 2627, 2687, 3177, 3331
HpySE526I	ACGT	9 cut(s)	126, 201, 237, 384, 1660, 2020, 2944, 3016, 3389
Hsp92I	GRCGYC	1 cut(s)	201
HspAI	GCGC	3 cut(s)	101, 103, 1429
KroI	GCCGGC	1 cut(s)	185
KroNI	GCCGGC	1 cut(s)	187
Ksp22I	TGATCA	1 cut(s)	1609
LguI	GCTCTTC	2 cut(s)	807, 1557
LmnI	GCTCC	4 cut(s)	366, 1078, 1357, 2011
Lsp1109I	GCAGC	2 cut(s)	3079, 3587
LweI	GCATC	9 cut(s)	288, 1157, 1204, 1957, 2644, 2965, 3222, 3232, 3325
MaeI	CTAG	5 cut(s)	1259, 1787, 2153, 3225, 3537
MaeII	ACGT	9 cut(s)	126, 201, 237, 384, 1660, 2020, 2944, 3016, 3389
MfeI	CAATTG	4 cut(s)	95, 636, 1438, 2571
MflI	RGATCY	5 cut(s)	646, 839, 881, 1304, 2720
MhlI	GDGCHC	3 cut(s)	1075, 1493, 3382
MlsI	TGGCCA	2 cut(s)	495, 1767
MluNI	TGGCCA	2 cut(s)	495, 1767
MlyI	GAGTC	6 cut(s)	153, 1789, 2221, 2486, 3035, 3323
MmeI	TCCRAC	2 cut(s)	622, 3117
Mox20I	TGGCCA	2 cut(s)	495, 1767
Mph1103I	ATGCAT	1 cut(s)	2083
MroNI	GCCGGC	1 cut(s)	185
MroXI	GAANNNNTTC	1 cut(s)	2490
MscI	TGGCCA	2 cut(s)	495, 1767
MseI	TTAA	8 cut(s)	1578, 1989, 2297, 2423, 2712, 2906, 2922, 3612
MslI	CAYNNNNRTG	2 cut(s)	1817, 2652
Msp20I	TGGCCA	2 cut(s)	495, 1767
MspA1I	CMGCKG	1 cut(s)	976
MspI	CCGG	4 cut(s)	156, 186, 275, 3042
MspR9I	CCNGG	1 cut(s)	1764
MunI	CAATTG	4 cut(s)	95, 636, 1438, 2571
Mva1269I	GAATGC	3 cut(s)	1456, 2107, 2490
MvaI	CCWGG	1 cut(s)	1764
MvnI	CGCG	2 cut(s)	103, 412
NaeI	GCCGGC	1 cut(s)	187
NcoI	CCATGG	2 cut(s)	686, 3271
NdeI	CATATG	1 cut(s)	919
NgoMIV	GCCGGC	1 cut(s)	185
NmeAIII	GCCGAG	1 cut(s)	1658
NmuCI	GTSAC	2 cut(s)	625, 2600
NsiI	ATGCAT	1 cut(s)	2083
NspI	RCATGY	1 cut(s)	3289
NspV	TTCGAA	1 cut(s)	87
PauI	GCGCGC	1 cut(s)	101
PceI	AGGCCT	2 cut(s)	83, 3609
PciSI	GCTCTTC	2 cut(s)	807, 1557
PctI	GAATGC	3 cut(s)	1456, 2107, 2490
PdiI	GCCGGC	1 cut(s)	187
PdmI	GAANNNNTTC	1 cut(s)	2490
PfeI	GAWTC	5 cut(s)	213, 785, 851, 1544, 3131
PflMI	CCANNNNNTGG	3 cut(s)	181, 641, 1774
PkrI	GCNGC	3 cut(s)	248, 3094, 3577
Ple19I	CGATCG	1 cut(s)	295
PleI	GAGTC	6 cut(s)	153, 1789, 2220, 2485, 3034, 3323
PpsI	GAGTC	6 cut(s)	153, 1789, 2220, 2485, 3034, 3323
Ppu21I	YACGTR	1 cut(s)	385
PpuMI	RGGWCCY	2 cut(s)	1637, 2443
PsiI	TTATAA	1 cut(s)	522
Psp5II	RGGWCCY	2 cut(s)	1637, 2443
Psp6I	CCWGG	1 cut(s)	1762
PspFI	CCCAGC	2 cut(s)	255, 2911
PspGI	CCWGG	1 cut(s)	1762
PspPPI	RGGWCCY	2 cut(s)	1637, 2443
PstI	CTGCAG	1 cut(s)	365
PstNI	CAGNNNCTG	2 cut(s)	2774, 3245
PsuI	RGATCY	5 cut(s)	646, 839, 881, 1304, 2720
PteI	GCGCGC	1 cut(s)	101
PvuI	CGATCG	1 cut(s)	295
RseI	CAYNNNNRTG	2 cut(s)	1817, 2652
SapI	GCTCTTC	2 cut(s)	807, 1557
SaqAI	TTAA	8 cut(s)	1578, 1989, 2297, 2423, 2712, 2906, 2922, 3612
SatI	GCNGC	3 cut(s)	247, 3093, 3576
SbfI	CCTGCAGG	1 cut(s)	365
ScaI	AGTACT	1 cut(s)	758
SchI	GAGTC	6 cut(s)	153, 1789, 2221, 2486, 3035, 3323
ScrFI	CCNGG	1 cut(s)	1764
SdaI	CCTGCAGG	1 cut(s)	365
SduI	GDGCHC	3 cut(s)	1075, 1493, 3382
SfaNI	GCATC	9 cut(s)	288, 1157, 1204, 1957, 2644, 2965, 3222, 3232, 3325
SfcI	CTRYAG	2 cut(s)	361, 2979
SfuI	TTCGAA	1 cut(s)	87
SinI	GGWCC	6 cut(s)	140, 690, 745, 1637, 1698, 2443
SmiMI	CAYNNNNRTG	2 cut(s)	1817, 2652
SmlI	CTYRAG	3 cut(s)	59, 1627, 2847
SmoI	CTYRAG	3 cut(s)	59, 1627, 2847
Sse8387I	CCTGCAGG	1 cut(s)	365
SseBI	AGGCCT	2 cut(s)	83, 3609
SspI	AATATT	4 cut(s)	1606, 2305, 2590, 3667
SspMI	CTAG	5 cut(s)	1259, 1787, 2153, 3225, 3537
StuI	AGGCCT	2 cut(s)	83, 3609
StyD4I	CCNGG	1 cut(s)	1762
StyI	CCWWGG	3 cut(s)	686, 2069, 3271
TaiI	ACGT	9 cut(s)	129, 204, 240, 387, 1663, 2023, 2947, 3019, 3392
TaqI	TCGA	8 cut(s)	87, 291, 1474, 1587, 1657, 1950, 2251, 2341
TatI	WGTACW	4 cut(s)	348, 594, 756, 2161
TauI	GCSGC	1 cut(s)	249
TfiI	GAWTC	5 cut(s)	213, 785, 851, 1544, 3131
Tru1I	TTAA	8 cut(s)	1578, 1989, 2297, 2423, 2712, 2906, 2922, 3612
Tru9I	TTAA	8 cut(s)	1578, 1989, 2297, 2423, 2712, 2906, 2922, 3612
TscAI	CASTG	7 cut(s)	556, 1054, 1245, 2164, 2195, 2751, 3327
TseFI	GTSAC	2 cut(s)	625, 2600
TseI	GCWGC	2 cut(s)	3092, 3575
Tsp45I	GTSAC	2 cut(s)	625, 2600
TspDTI	ATGAA	9 cut(s)	369, 457, 906, 936, 984, 2255, 2483, 2636, 3520
TspGWI	ACGGA	3 cut(s)	1007, 2453, 3097
TspRI	CASTG	7 cut(s)	556, 1054, 1245, 2164, 2195, 2751, 3327
Van91I	CCANNNNNTGG	3 cut(s)	181, 641, 1774
VpaK11BI	GGWCC	6 cut(s)	140, 690, 745, 1637, 1698, 2443
XagI	CCTNNNNNAGG	1 cut(s)	1889
XapI	RAATTY	8 cut(s)	661, 727, 865, 935, 1756, 3069, 3368, 3415
XbaI	TCTAGA	1 cut(s)	3536
XceI	RCATGY	1 cut(s)	3289
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1503, 3278
XmnI	GAANNNNTTC	1 cut(s)	2490
XspI	CTAG	5 cut(s)	1259, 1787, 2153, 3225, 3537
ZraI	GACGTC	1 cut(s)	202
ZrmI	AGTACT	1 cut(s)	758
Zsp2I	ATGCAT	1 cut(s)	2083

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.