Rorug04G0083900

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009542
Physical Location & Seq
Reverse (-)
12855974 .. 12858785
2812 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0083900.1

Sequence Viewer

Length: 711 bp
ATGGTGGAAGTGAGGAACAAGCAGGTGATATTGAAGAAGTATGTGTCGGCCGGGCTCCCAAAAGAATCTGACATGGCCGTTGTCACTACCACCACCAAACTGAAGCTTCCAGAAGGCTCCGGCGGGGTTCTGGTGAAGAACCTCTACTTGTCCTGCGATCCTTACATGAGAGGCCACGTGCGAACCTGCTTCTTATGTCGATCCCTTCAACCCCAGCAAGCGTATCAGCAGTATAAACTCTGGAATTCCTACCTTAGAGCTGTTATGGAGTGTGACATGAAAGCAGTTAGCAAAGGTAATAAGAGCAAAGTTGAAGTTTTGGAGACTTGCTTGCAGCAAATGAAAGCCTGCTTCCTTGATGTAAGCTTATCAAATAGATCTGGTGGAGATGAGCAGCATGCATCTGGAGAGTTCATTCGGCGATGTGGACTCTGCCAGGAAGCAGATATGGTGCTTAGGAAAAATCAGGTTGATTTGCTGGAGAAGAAGTTTGGCTTTGATGAGAAAGCCGGTAAAATGGGTCACAAAAGAGGTGGACTTGTTAGCATGCACAAGTTTGGTTTATGGGAAATAAGTGAAGGTTTCACTCTTCAATACTTTCACAACAGAGCAATCGAACAGTGGATGCAGACGACAACTTTTAGTCCTCTCCAGTTGCACTGCTTTTCTTATAATGGCATTTTCTACTTGAGAAAAGCTTGGGTTTCTTAG

Protein Analysis

236

Amino Acids

27.01

Weight (kDa)

9.23

Isoelectric Point (pI)

59.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 7 - 61 7.4e-08 N-terminal domain of oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 672
AarI CACCTGC 1 cut(s) 13
Acc36I ACCTGC 2 cut(s) 13, 194
AciI CCGC 1 cut(s) 123
AclWI GGATC 2 cut(s) 152, 195
AcoI YGGCCR 2 cut(s) 48, 75
AcsI RAATTY 1 cut(s) 244
AcuI CTGAAG 1 cut(s) 122
AcvI CACGTG 1 cut(s) 178
AgsI TTSAA 4 cut(s) 34, 209, 314, 593
AjnI CCWGG 1 cut(s) 435
AluBI AGCT 4 cut(s) 106, 260, 366, 698
AluI AGCT 4 cut(s) 106, 260, 366, 698
Alw26I GTCTC 1 cut(s) 317
AlwI GGATC 2 cut(s) 152, 195
AoxI GGCC 3 cut(s) 48, 75, 172
ApeKI GCWGC 2 cut(s) 334, 394
ApoI RAATTY 1 cut(s) 244
AsuC2I CCSGG 1 cut(s) 52
AsuHPI GGTGA 2 cut(s) 37, 145
BanII GRGCYC 1 cut(s) 57
BarI GAAGNNNNNNTAC 2 cut(s) 128, 160
BbrPI CACGTG 1 cut(s) 178
BbvI GCAGC 2 cut(s) 346, 406
BceAI ACGGC 1 cut(s) 62
BciT130I CCWGG 1 cut(s) 437
BcnI CCSGG 1 cut(s) 52
BcoDI GTCTC 1 cut(s) 317
BfuAI ACCTGC 2 cut(s) 13, 194
BglII AGATCT 1 cut(s) 377
BisI GCNGC 2 cut(s) 335, 395
BlsI GCNGC 2 cut(s) 336, 396
Bme1390I CCNGG 2 cut(s) 52, 437
BmiI GGNNCC 2 cut(s) 56, 118
BmrFI CCNGG 2 cut(s) 52, 437
BmsI GCATC 2 cut(s) 410, 615
BpmI CTGGAG 3 cut(s) 426, 500, 635
Bpu10I CCTNAGC 1 cut(s) 455
BpuEI CTTGAG 1 cut(s) 709
BpuMI CCSGG 1 cut(s) 52
BsaAI YACGTR 1 cut(s) 178
BsaXI ACNNNNNCTCC 2 cut(s) 473, 503
Bse118I RCCGGY 1 cut(s) 509
Bse1I ACTGG 1 cut(s) 652
BseBI CCWGG 1 cut(s) 437
BseGI GGATG 1 cut(s) 630
BseNI ACTGG 1 cut(s) 652
BseX3I CGGCCG 1 cut(s) 48
BseXI GCAGC 2 cut(s) 346, 406
BseYI CCCAGC 1 cut(s) 213
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 3 cut(s) 50, 77, 174
BsiEI CGRYCG 1 cut(s) 51
BsiSI CCGG 3 cut(s) 51, 120, 510
BsmAI GTCTC 1 cut(s) 317
BsnI GGCC 3 cut(s) 50, 77, 174
Bsp1286I GDGCHC 1 cut(s) 57
Bsp143I GATC 3 cut(s) 157, 200, 377
BspACI CCGC 1 cut(s) 123
BspANI GGCC 3 cut(s) 50, 77, 174
BspLI GGNNCC 2 cut(s) 56, 118
BspMI ACCTGC 2 cut(s) 13, 194
BspPI GGATC 2 cut(s) 152, 195
BsrFI RCCGGY 1 cut(s) 509
BsrI ACTGG 1 cut(s) 652
BssAI RCCGGY 1 cut(s) 509
BssMI GATC 3 cut(s) 157, 200, 377
Bst2UI CCWGG 1 cut(s) 437
Bst4CI ACNGT 1 cut(s) 621
Bst6I CTCTTC 1 cut(s) 594
BstBAI YACGTR 1 cut(s) 178
BstC8I GCNNGC 5 cut(s) 219, 332, 349, 399, 548
BstDEI CTNAG 3 cut(s) 254, 455, 708
BstF5I GGATG 1 cut(s) 630
BstKTI GATC 3 cut(s) 160, 203, 380
BstMAI GTCTC 1 cut(s) 317
BstMBI GATC 3 cut(s) 157, 200, 377
BstMCI CGRYCG 1 cut(s) 51
BstNI CCWGG 1 cut(s) 437
BstNSI RCATGY 2 cut(s) 401, 550
BstSCI CCNGG 2 cut(s) 50, 435
BstV1I GCAGC 2 cut(s) 346, 406
BstX2I RGATCY 1 cut(s) 377
BstYI RGATCY 1 cut(s) 377
BstZI CGGCCG 1 cut(s) 48
BsuRI GGCC 3 cut(s) 50, 77, 174
BtgZI GCGATG 1 cut(s) 436
BtsCI GGATG 1 cut(s) 630
BtsI GCAGTG 1 cut(s) 658
BtsIMutI CAGTG 2 cut(s) 626, 658
BveI ACCTGC 2 cut(s) 13, 194
Cac8I GCNNGC 5 cut(s) 219, 332, 349, 399, 548
Cfr10I RCCGGY 1 cut(s) 509
CspCI CAANNNNNGTGG 2 cut(s) 514, 549
CviAII CATG 5 cut(s) 73, 166, 277, 398, 547
DdeI CTNAG 3 cut(s) 254, 455, 708
DpnI GATC 3 cut(s) 159, 202, 379
DpnII GATC 3 cut(s) 157, 200, 377
EaeI YGGCCR 2 cut(s) 48, 75
EagI CGGCCG 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
EclXI CGGCCG 1 cut(s) 48
Eco24I GRGCYC 1 cut(s) 57
Eco52I CGGCCG 1 cut(s) 48
Eco57I CTGAAG 1 cut(s) 122
Eco72I CACGTG 1 cut(s) 178
EcoRI GAATTC 1 cut(s) 244
EcoRII CCWGG 1 cut(s) 435
EcoT22I ATGCAT 1 cut(s) 403
EcoT38I GRGCYC 1 cut(s) 57
FaeI CATG 5 cut(s) 76, 169, 280, 401, 550
FalI AAGNNNNNCTT 2 cut(s) 479, 511
FatI CATG 5 cut(s) 72, 165, 276, 397, 546
FauI CCCGC 1 cut(s) 116
Fnu4HI GCNGC 2 cut(s) 335, 395
FokI GGATG 1 cut(s) 637
FriOI GRGCYC 1 cut(s) 57
Fsp4HI GCNGC 2 cut(s) 335, 395
GluI GCNGC 2 cut(s) 335, 395
GsaI CCCAGC 1 cut(s) 217
GsuI CTGGAG 3 cut(s) 426, 500, 635
HaeIII GGCC 3 cut(s) 50, 77, 174
HapII CCGG 3 cut(s) 51, 120, 510
Hin1II CATG 5 cut(s) 76, 169, 280, 401, 550
HindIII AAGCTT 3 cut(s) 104, 364, 696
HinfI GANTC 2 cut(s) 65, 429
HpaII CCGG 3 cut(s) 51, 120, 510
HphI GGTGA 2 cut(s) 37, 145
Hpy166II GTNNAC 2 cut(s) 428, 536
Hpy188I TCNGA 1 cut(s) 70
Hpy188III TCNNGA 3 cut(s) 110, 241, 405
Hpy8I GTNNAC 2 cut(s) 428, 536
HpyAV CCTTC 3 cut(s) 107, 215, 572
HpyCH4III ACNGT 1 cut(s) 621
HpyCH4IV ACGT 1 cut(s) 177
HpyCH4V TGCA 5 cut(s) 334, 401, 550, 628, 658
HpyF3I CTNAG 3 cut(s) 254, 455, 708
HpySE526I ACGT 1 cut(s) 177
Hsp92II CATG 5 cut(s) 76, 169, 280, 401, 550
Kzo9I GATC 3 cut(s) 157, 200, 377
LmnI GCTCC 2 cut(s) 60, 122
Lsp1109I GCAGC 2 cut(s) 346, 406
LweI GCATC 2 cut(s) 410, 615
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 3 cut(s) 82, 272, 521
MalI GATC 3 cut(s) 159, 202, 379
MboI GATC 3 cut(s) 157, 200, 377
MboII GAAGA 4 cut(s) 46, 148, 496, 581
MflI RGATCY 1 cut(s) 377
MhlI GDGCHC 1 cut(s) 57
MluCI AATT 1 cut(s) 244
MlyI GAGTC 1 cut(s) 423
MnlI CCTC 5 cut(s) 6, 152, 164, 524, 657
Mph1103I ATGCAT 1 cut(s) 403
MspI CCGG 3 cut(s) 51, 120, 510
MspR9I CCNGG 2 cut(s) 52, 437
MvaI CCWGG 1 cut(s) 437
NciI CCSGG 1 cut(s) 52
NdeII GATC 3 cut(s) 157, 200, 377
NlaIII CATG 5 cut(s) 76, 169, 280, 401, 550
NlaIV GGNNCC 2 cut(s) 56, 118
NmuCI GTSAC 3 cut(s) 82, 272, 521
NsiI ATGCAT 1 cut(s) 403
NspI RCATGY 2 cut(s) 401, 550
PaeI GCATGC 2 cut(s) 401, 550
PaqCI CACCTGC 1 cut(s) 13
PfeI GAWTC 1 cut(s) 65
PkrI GCNGC 2 cut(s) 336, 396
PleI GAGTC 1 cut(s) 423
PmaCI CACGTG 1 cut(s) 178
PmlI CACGTG 1 cut(s) 178
PpsI GAGTC 1 cut(s) 423
Ppu21I YACGTR 1 cut(s) 178
PsiI TTATAA 1 cut(s) 672
Psp6I CCWGG 1 cut(s) 435
PspCI CACGTG 1 cut(s) 178
PspFI CCCAGC 1 cut(s) 213
PspGI CCWGG 1 cut(s) 435
PspN4I GGNNCC 2 cut(s) 56, 118
PsuI RGATCY 1 cut(s) 377
SatI GCNGC 2 cut(s) 335, 395
Sau3AI GATC 3 cut(s) 157, 200, 377
SchI GAGTC 1 cut(s) 423
ScrFI CCNGG 2 cut(s) 52, 437
SduI GDGCHC 1 cut(s) 57
SfaNI GCATC 2 cut(s) 410, 615
SmlI CTYRAG 1 cut(s) 688
SmoI CTYRAG 1 cut(s) 688
SphI GCATGC 2 cut(s) 401, 550
Sse9I AATT 1 cut(s) 244
SsiI CCGC 1 cut(s) 123
StyD4I CCNGG 2 cut(s) 50, 435
TaaI ACNGT 1 cut(s) 621
TaiI ACGT 1 cut(s) 180
TaqI TCGA 2 cut(s) 199, 615
TasI AATT 1 cut(s) 244
TfiI GAWTC 1 cut(s) 65
TscAI CASTG 2 cut(s) 626, 665
TseFI GTSAC 3 cut(s) 82, 272, 521
TseI GCWGC 2 cut(s) 334, 394
Tsp45I GTSAC 3 cut(s) 82, 272, 521
TspDTI ATGAA 3 cut(s) 293, 356, 403
TspRI CASTG 2 cut(s) 626, 665
XapI RAATTY 1 cut(s) 244
XceI RCATGY 2 cut(s) 401, 550
Zsp2I ATGCAT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.