Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0009542

Physical Location & Seq

Genomic Coordinates Reverse (-)

65640565 .. 65648867

Gene Structure 8303 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000035346

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3702 bp

ATGAATTGTGTATTGCCATCTCGTGTATTTCATTTGGGCATTCAAGTTGACGGTCAGTGTGTTGTAGTTGCTGGTTTTCTTGGTGTTTTCGTCTGTCTACTTTGTCGAGCATTCATTTCTTGTTGTAAAGCTGCTTTGCACTGTGTGGTACGTGTTTCCTTTGCAAATATTGTGGAGGAGGTATGTGAAAGCAAGGACTTCTCCTTCCCCAAACATGAGGAGAAGATCCTCGAGTTCTGGTCTGAAATCAATGCCTTTGAGACCCAATTGGCTCGAACCAAGGACATGCCGGAGTATGTCTTCTACGACGGTCCAGCTTTTGCCACTGGCCTCCCACACTATGGCCACCTATTGGCTGGCACCATCAAGGACATTGTCACGCGCTACCAGTCCATGACTGGCCACCATGTCACGCGCCGCTTTGGCTGGGACTGCCATGGCTTGCCTGTTGAGACCGAGATCGATAAGAAGCTCAACATCAAGACCCGAGAAGATGTTCTGAACCTGGGAATTGATGCCTACAATGAAGAATGTAGGAGCTTTGTTACTCGATATGTGGAGGAATGGGAGAAGGTGGTTACTCGCTCGGGGCGGTGGATCGATTTCAGGAATGATTATAAGACTACGGACTTGAAGTTCATGGAGAGTGTGTGGTGGGTTTTGCTCAATTATATGAAAAGAGTCTTCACAGGTTGCAAGACACCACTCTCCAATTTTGAGGCCAGTCAAAATCACAAGGATGTGTCTGATCCTGAAATAATGGTAGCTTTCCCAATTGTCGGTGATCCGTATAAGGCAGCGTTGGTGGCTTGGACAACTACTCCATGGACCCTTTCTAGCAATTTAGCCCTGTGTGTCAATCCAAATTTAACCTATGCGAAGGTTTGCAACAGGTGTTCTGGAAGAGTTTATGTCGTTGCTGAATCCCGGATTTCATCTCTACCTAGTGAGAGACCCAGAGGAGCTGCTTATGACGGTTCAAATATTTATGAAGTGTTGGAGAGATTCTCAGGGACTACACTAGTGGGAAAGAAGTATGAACCACTTTTTGATTACTTCATGGATGGTACTGGAGTTGTTCACTGTGCTCCTGCATTTGGTGAGGATGACTACCGCGTTTGCCTTGCAAATGAAGTAATCAAAAAGGGAGAGGGCTTGATTGTACCAGTTGATGAGAATGGGTGCTTTACAGAAAGAGTTGCTGACTTCAGCAACCTCTATGTCAAGGAGGCAGATAAGGCTATTATTGAAGCAGTGAGGCTGAAGGGAAGGCTTGTTAAAACAAACACACCACTTATCTACCGAGCTGTTCCAAGCTGGTTTATCCGGGTGGAGCAGTTGAAAGAAAAGTTATTGGAAAACAACAAGCAGACATATTGGGTTCCTGATTTTGTGAAGGAAAATCGATTCCATAATTGGCTAGAAAATGCAAGAGATTGGGCAGTTAGCAGAAGTAGATTTTGGGGTACTCCTCTTCCTGTGTGGGTTAGTGAGGATGGTGAGGAAGTACTGGTTTCTGATCTGCACCGCCACTACATTGATCATATCACTATTCCATCGGCGCGTGGCCCTGAATTTGGTTATCTTCGACGTGTAGACGATGTGTTTGATTGTTGGTTTGAGAGTGGATCCATGCCTTATGCTTATATCCACTATCCTTTCGACAATGTGGAGCTTTTTGAGAAAAACTTTCCTGGGAACTTTGTGGCCGAAGGGCTTGATCAGACTCGCGGATGGTTCTACACTCTTATGGTGCTATCTACTGCATTATTTGGGAAACCTGCTTTTAAGAACCTCATTTGCAATGGACTTGTTCTTGCTGATGATGGGAAAAAGATGGGAAAAAGTTTAAAAAACTTTCCTCCAGTTATGGAGGTTCTCGATCAATATGGGGCTGATGCAGTTCGAATGTATATTATTAACTCTCCTGTTGTTCGTGCAGAACCATTACGATTCAAGAAGGAAGGAGTATATGGAGTTGTCAAGGATGTATTTCTGCCTTGGTACAATGCATATAGGTTCCTCGTTCAACAATCAAAAAGACTCAATGCGCCTTTCCTCCCTCTTGATCAAACTACTGTACAGAAGTCATCTAATGTGCTTGACCAGTGGATCAACTCAGCCACACAGTCTCTTGTGCATTTTGTCTGTCAAGAGATGGATGCTTACCGACTCTACACGGTGGTGCCATATCTTTTGAAGTTTCTTGACAACTTGACAAACATCTATGTAAGGTTGAATCGCACGAGGCTGAAGCTAAAGGGTACTAACGAAGAAGATTGCAGGATGGGACTCTCTACTCGTTTCCATGTCCTTTTAGTGTCATGTAAAGTGATGGCACCATTAACACCGTTCTTCACTGAAGCCCTTTACCAAAATATGCGAAAAGTTCTGAATGATGCAGAGGAAAGCATTCATTACTGCAGTTTCCCTCAAGCACAAGGAAAAAGGGACGCTCGTATCAAGCAAAGTGTTGCAAGGATGAAGACAGTAATTGATCATGCTCGTAATATTCGCGAACGTCACAACAAGCCTCTCAAAACACCCCTAAAAGAGATGGTCATTGTTCACCCTGATGCAGACTTTCTTGAAGACATTGATGCCAAGCTAAGAGAATATGTGCTTGAAGAACTTAATGTGCAATCTCTAGACCTGTGCAATGATACACTGAAGTATACGTCATCGCGCGCAGAGCCTGACTTCAGTGTGTTAGGTAAGCGGGTAGGAAAATCTATGGGGGTTGTTTCCAAACAAGTCAAGGCCATGTCACATGAGAGTATCTTAGAATTCGAGAAAACTAGAGAGGTTACTTTTTGTGGTCATTCTCTGAAGCTGAATGATATTAAGGTTGTTAGAGACTTCAAATGTCCTGATGGCACAACAGAGAAGGAGATTGATGCATCAGGAGATGGTGATGTTCTGGTGATCTTGGATTTGCATCAAGACGAATCGTTGTTTGTGGCAGTTGTTGCTCGTGAGATTGTTAGCAGAGTTCAGAAGTTGAGGAAGAAATCGGGCCTTGGACTCACTGACATTGTGGAGGTGTACTTTGAGTCTTTGGATGAAGATAAATCAGTCTCGGACCGAGTTTTGCATTTGCAGGAACAATACATTAAGGATGCCATAGGTTCACCAATGCTTCCTTGCGGTGTGAGGCCATCACATGCTGCTGTGGTTGGGGAGGAGAATTTCCGTGGAGTATCTGGAATGATTGGAAGCTTGAATCATGCTGAAGATCTGGACAAACCGTTGTTGGATCCTGAGAATTTCAATAGAGATGGCATCGAATTGGAGCACTTACCCTTGGAAGAAGTTTTTGAACAACTGAGAACATCAGCAAGAGGACTTTCATCCGATGATGCTGAATGCAGATTGCACATTTTTGTCAACATCTTTCCACCTCTGAATGAGAACAAAATTTTGAAGTTTCTTAGCTTTATGTGGAACCCCTTGTCATGGGTTATGGAAGCTGCAGCAGTTATGGCACTTGTCCTTGCTAATAGGGGAGGTAAGGGTCCTGATTGGCAAGACTTTGTTGGGATTATTGTCCTATTAATAATCAATTTAACAATTAGTTTCATAGAGGAGAATAATGCGGGAAATGCTGCGTCAGCTCTTATGGAACGTTATCTCCAAAGACAAGGGTATGCCTCTTTGATTGATGCCTTCCGCTCACGGAGTTTGGCTATGACAGTTTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004822 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006428 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0017101 GO:0019538 GO:0019752 GO:0019899 GO:0032991 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0051020 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1

Protein Analysis

1234

Amino Acids

140.47

Weight (kDa)

5.97

Isoelectric Point (pI)

37.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	75 - 648	5.2e-132	tRNA synthetases class I (I, L, M and V)
Anticodon_1	PF08264	702 - 852	3.5e-22	Anticodon-binding domain of tRNA ligase
DUF5915	PF19302	872 - 1026	5.1e-22	Domain of unknown function (DUF5915)
Cation_ATPase_N	PF00690	1098 - 1161	1.1e-06	Cation transporter/ATPase, N-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0009542)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G10320
fragaria_vesca	FvH4_4g11470
malus_domestica	MD04G1037200.v1.1 MD09G1065200.v1.1
prunus_persica	Prupe.1G186600_v2.0.a1 Prupe.1G186600_v2.0.a1
pyrus_communis	pycom04g03030 pycom111g05510
rosa_chinensis	RchiOBHm_Chr4g0408181
rosa_laevigata	RLG00000008666 RLG00000035346
rosa_multiflora	Rmu_ssc0000039.1_g000001
rosa_roxburghii	Rroxscaffold_1G00020510 Rroxscaffold_5G00352680
rosa_rugosa	Rorug04G0083900 Rorug04G0084000 Rorug05G0328300
rosa_samantha	Rh4AG148700 Rh4BG145500 Rh4CG155200 Rh4DG142000
rosa_wichuraiana	Rw4G012140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	618
Acc36I	ACCTGC	1 cut(s)	1789
AccB1I	GGYRCC	3 cut(s)	359, 2185, 2338
AccB7I	CCANNNNNTGG	2 cut(s)	341, 352
AccBSI	CCGCTC	1 cut(s)	3672
AccI	GTMKAC	3 cut(s)	97, 1596, 2675
AccII	CGCG	8 cut(s)	382, 415, 1116, 1564, 1731, 2517, 2686, 2688
AciI	CCGC	9 cut(s)	418, 592, 1114, 1528, 1731, 2719, 3147, 3596, 3670
AclI	AACGTT	1 cut(s)	3625
AclWI	GGATC	9 cut(s)	220, 605, 743, 779, 1623, 1636, 2120, 3251, 3264
AcoI	YGGCCR	3 cut(s)	343, 400, 1707
AcsI	RAATTY	6 cut(s)	865, 1574, 2786, 3187, 3265, 3417
AcuI	CTGAAG	8 cut(s)	1192, 1283, 2273, 2382, 2686, 2690, 2849, 3252
AdeI	CACNNNGTG	1 cut(s)	145
AfaI	GTAC	9 cut(s)	150, 1069, 1164, 1468, 1509, 2006, 2082, 2266, 3047
AfiI	CCNNNNNNNGG	6 cut(s)	341, 352, 779, 1097, 1577, 3456
AflIII	ACRYGT	2 cut(s)	151, 1591
AhlI	ACTAGT	1 cut(s)	1021
AjiI	CACGTC	1 cut(s)	1592
AjnI	CCWGG	2 cut(s)	504, 1693
AjuI	GAANNNNNNNTTGG	2 cut(s)	1444, 1476
AleI	CACNNNNGTG	1 cut(s)	2183
AloI	GAACNNNNNNTCC	4 cut(s)	620, 652, 3615, 3647
Alw21I	GWGCWC	2 cut(s)	1090, 3297
Alw26I	GTCTC	6 cut(s)	254, 446, 946, 2136, 2850, 3082
AlwI	GGATC	9 cut(s)	220, 605, 743, 779, 1623, 1636, 2120, 3251, 3264
AlwNI	CAGNNNCTG	1 cut(s)	2696
Ama87I	CYCGRG	3 cut(s)	230, 486, 586
AoxI	GGCC	9 cut(s)	328, 343, 400, 720, 1567, 1707, 2760, 3016, 3155
ApeKI	GCWGC	7 cut(s)	131, 797, 965, 3167, 3470, 3473, 3605
ApoI	RAATTY	6 cut(s)	865, 1574, 2786, 3187, 3265, 3417
ArsI	GACNNNNNNTTYG	2 cut(s)	1589, 1621
AseI	ATTAAT	1 cut(s)	3554
Asp700I	GAANNNNTTC	2 cut(s)	495, 2412
AspLEI	GCGC	6 cut(s)	384, 417, 1564, 2053, 2688, 2690
AspS9I	GGNCC	6 cut(s)	311, 828, 1568, 3016, 3082, 3515
AsuC2I	CCSGG	2 cut(s)	928, 1328
AsuHPI	GGTGA	7 cut(s)	794, 1112, 1511, 2561, 2924, 2935, 3123
AsuII	TTCGAA	1 cut(s)	1906
AvaI	CYCGRG	3 cut(s)	230, 486, 586
AvaII	GGWCC	4 cut(s)	311, 828, 3082, 3515
BaeI	ACNNNNGTAYC	4 cut(s)	1059, 1092, 2443, 2476
BalI	TGGCCA	2 cut(s)	345, 402
BamHI	GGATCC	2 cut(s)	1628, 3256
BanI	GGYRCC	3 cut(s)	359, 2185, 2338
BauI	CACGAG	3 cut(s)	21, 2245, 2973
BbsI	GAAGAC	4 cut(s)	292, 676, 2492, 2598
Bbv12I	GWGCWC	2 cut(s)	1090, 3297
BbvI	GCAGC	7 cut(s)	118, 809, 952, 3154, 3457, 3485, 3592
BciT130I	CCWGG	2 cut(s)	506, 1695
BclI	TGATCA	4 cut(s)	1540, 1720, 2068, 2497
BcnI	CCSGG	2 cut(s)	928, 1328
BcoDI	GTCTC	6 cut(s)	254, 446, 946, 2136, 2850, 3082
BcuI	ACTAGT	1 cut(s)	1021
BfaI	CTAG	6 cut(s)	837, 945, 1022, 1421, 2648, 2799
BfmI	CTRYAG	2 cut(s)	2422, 3471
BfuAI	ACCTGC	1 cut(s)	1789
BglI	GCCNNNNNGGC	1 cut(s)	423
BglII	AGATCT	1 cut(s)	3235
BisI	GCNGC	8 cut(s)	132, 418, 798, 966, 3168, 3471, 3474, 3606
BlsI	GCNGC	8 cut(s)	133, 419, 799, 967, 3169, 3472, 3475, 3607
BmcAI	AGTACT	1 cut(s)	1509
Bme1390I	CCNGG	4 cut(s)	506, 928, 1328, 1695
Bme18I	GGWCC	4 cut(s)	311, 828, 3082, 3515
BmeT110I	CYCGRG	3 cut(s)	230, 486, 586
BmgBI	CACGTC	1 cut(s)	1592
BmgT120I	GGNCC	6 cut(s)	311, 828, 1568, 3016, 3082, 3515
BmrFI	CCNGG	4 cut(s)	506, 928, 1328, 1695
BpiI	GAAGAC	4 cut(s)	292, 676, 2492, 2598
BplI	GAGNNNNNCTC	2 cut(s)	992, 1024
BpmI	CTGGAG	2 cut(s)	1092, 1848
Bpu14I	TTCGAA	1 cut(s)	1906
BpuEI	CTTGAG	1 cut(s)	2418
BpuMI	CCSGG	2 cut(s)	928, 1328
Bsa29I	ATCGAT	3 cut(s)	462, 600, 1405
BsaAI	YACGTR	1 cut(s)	152
BsaBI	GATNNNNATC	1 cut(s)	2937
BsaI	GGTCTC	3 cut(s)	254, 446, 946
BsaJI	CCNNGG	9 cut(s)	279, 436, 505, 824, 1694, 2000, 3019, 3193, 3303
BsaXI	ACNNNNNCTCC	6 cut(s)	635, 665, 805, 835, 3615, 3645
Bsc4I	CCNNNNNNNGG	6 cut(s)	341, 352, 779, 1097, 1577, 3456
Bse1I	ACTGG	9 cut(s)	331, 388, 403, 723, 1075, 1166, 1515, 1865, 2107
Bse3DI	GCAATG	2 cut(s)	1810, 2665
Bse8I	GATNNNNATC	1 cut(s)	2937
BseBI	CCWGG	2 cut(s)	506, 1695
BseCI	ATCGAT	3 cut(s)	462, 600, 1405
BseDI	CCNNGG	9 cut(s)	279, 436, 505, 824, 1694, 2000, 3019, 3193, 3303
BseJI	GATNNNNATC	1 cut(s)	2937
BseLI	CCNNNNNNNGG	6 cut(s)	341, 352, 779, 1097, 1577, 3456
BseMI	GCAATG	2 cut(s)	1810, 2665
BseMII	CTCAG	4 cut(s)	1023, 2133, 3252, 3317
BseNI	ACTGG	9 cut(s)	331, 388, 403, 723, 1075, 1166, 1515, 1865, 2107
BsePI	GCGCGC	1 cut(s)	2686
BseRI	GAGGAG	6 cut(s)	191, 233, 975, 1461, 3197, 3599
BseXI	GCAGC	7 cut(s)	118, 809, 952, 3154, 3457, 3485, 3592
BseYI	CCCAGC	1 cut(s)	426
BsgI	GTGCAG	2 cut(s)	1508, 1959
Bsh1236I	CGCG	8 cut(s)	382, 415, 1116, 1564, 1731, 2517, 2686, 2688
BshFI	GGCC	9 cut(s)	330, 345, 402, 722, 1569, 1709, 2762, 3018, 3157
BshNI	GGYRCC	3 cut(s)	359, 2185, 2338
BshVI	ATCGAT	3 cut(s)	462, 600, 1405
BsiHKAI	GWGCWC	2 cut(s)	1090, 3297
BsiHKCI	CYCGRG	3 cut(s)	230, 486, 586
BsiSI	CCGG	3 cut(s)	290, 928, 1327
BslFI	GGGAC	4 cut(s)	443, 1027, 2304, 2465
BslI	CCNNNNNNNGG	6 cut(s)	341, 352, 779, 1097, 1577, 3456
BsmAI	GTCTC	6 cut(s)	254, 446, 946, 2136, 2850, 3082
BsmFI	GGGAC	4 cut(s)	443, 1027, 2304, 2465
BsmI	GAATGC	4 cut(s)	39, 110, 2412, 3371
BsnI	GGCC	9 cut(s)	330, 345, 402, 722, 1569, 1709, 2762, 3018, 3157
Bso31I	GGTCTC	3 cut(s)	254, 446, 946
BsoBI	CYCGRG	3 cut(s)	230, 486, 586
Bsp119I	TTCGAA	1 cut(s)	1906
Bsp1286I	GDGCHC	2 cut(s)	1090, 3297
Bsp1407I	TGTACA	1 cut(s)	2080
Bsp19I	CCATGG	2 cut(s)	436, 824
Bsp68I	TCGCGA	1 cut(s)	2517
BspACI	CCGC	9 cut(s)	418, 592, 1114, 1528, 1731, 2719, 3147, 3596, 3670
BspANI	GGCC	9 cut(s)	330, 345, 402, 722, 1569, 1709, 2762, 3018, 3157
BspCNI	CTCAG	4 cut(s)	1022, 2132, 3253, 3318
BspDI	ATCGAT	3 cut(s)	462, 600, 1405
BspFNI	CGCG	8 cut(s)	382, 415, 1116, 1564, 1731, 2517, 2686, 2688
BspMAI	CTGCAG	2 cut(s)	2426, 3475
BspMI	ACCTGC	1 cut(s)	1789
BspPI	GGATC	9 cut(s)	220, 605, 743, 779, 1623, 1636, 2120, 3251, 3264
BspT104I	TTCGAA	1 cut(s)	1906
BspT107I	GGYRCC	3 cut(s)	359, 2185, 2338
BspTNI	GGTCTC	3 cut(s)	254, 446, 946
BsrBI	CCGCTC	1 cut(s)	3672
BsrDI	GCAATG	2 cut(s)	1810, 2665
BsrGI	TGTACA	1 cut(s)	2080
BsrI	ACTGG	9 cut(s)	331, 388, 403, 723, 1075, 1166, 1515, 1865, 2107
BssECI	CCNNGG	9 cut(s)	279, 436, 505, 824, 1694, 2000, 3019, 3193, 3303
BssHII	GCGCGC	1 cut(s)	2686
BssNAI	GTATAC	1 cut(s)	2676
BssSI	CACGAG	3 cut(s)	21, 2245, 2973
BssT1I	CCWWGG	6 cut(s)	279, 436, 824, 2000, 3019, 3303
Bst1107I	GTATAC	1 cut(s)	2676
Bst2BI	CACGAG	3 cut(s)	21, 2245, 2973
Bst2UI	CCWGG	2 cut(s)	506, 1695
Bst6I	CTCTTC	2 cut(s)	898, 1479
BstAPI	GCANNNNNTGC	1 cut(s)	2969
BstAUI	TGTACA	1 cut(s)	2080
BstBAI	YACGTR	1 cut(s)	152
BstBI	TTCGAA	1 cut(s)	1906
BstC8I	GCNNGC	3 cut(s)	358, 443, 2688
BstDEI	CTNAG	7 cut(s)	1009, 2119, 2609, 2782, 3261, 3326, 3431
BstDSI	CCRYGG	3 cut(s)	436, 824, 3193
BstFNI	CGCG	8 cut(s)	382, 415, 1116, 1564, 1731, 2517, 2686, 2688
BstHHI	GCGC	6 cut(s)	384, 417, 1564, 2053, 2688, 2690
BstMAI	GTCTC	6 cut(s)	254, 446, 946, 2136, 2850, 3082
BstMWI	GCNNNNNNNGC	9 cut(s)	423, 432, 806, 1238, 2692, 2969, 3482, 3602, 3611
BstNI	CCWGG	2 cut(s)	506, 1695
BstNSI	RCATGY	2 cut(s)	289, 3167
BstSCI	CCNGG	4 cut(s)	504, 926, 1326, 1693
BstSFI	CTRYAG	2 cut(s)	2422, 3471
BstUI	CGCG	8 cut(s)	382, 415, 1116, 1564, 1731, 2517, 2686, 2688
BstV1I	GCAGC	7 cut(s)	118, 809, 952, 3154, 3457, 3485, 3592
BstV2I	GAAGAC	4 cut(s)	292, 676, 2492, 2598
BstX2I	RGATCY	4 cut(s)	225, 1628, 3235, 3256
BstYI	RGATCY	4 cut(s)	225, 1628, 3235, 3256
BstZ17I	GTATAC	1 cut(s)	2676
Bsu15I	ATCGAT	3 cut(s)	462, 600, 1405
BsuRI	GGCC	9 cut(s)	330, 345, 402, 722, 1569, 1709, 2762, 3018, 3157
BsuTUI	ATCGAT	3 cut(s)	462, 600, 1405
BtgI	CCRYGG	3 cut(s)	436, 824, 3193
BtgZI	GCGATG	1 cut(s)	2667
BtrI	CACGTC	1 cut(s)	1592
BtsI	GCAGTG	1 cut(s)	1260
BtuMI	TCGCGA	1 cut(s)	2517
BveI	ACCTGC	1 cut(s)	1789
Cac8I	GCNNGC	3 cut(s)	358, 443, 2688
CaiI	CAGNNNCTG	1 cut(s)	2696
CfoI	GCGC	6 cut(s)	384, 417, 1564, 2053, 2688, 2690
Cfr13I	GGNCC	6 cut(s)	311, 828, 1568, 3016, 3082, 3515
ClaI	ATCGAT	3 cut(s)	462, 600, 1405
CpoI	CGGWCCG	1 cut(s)	3082
CseI	GACGC	2 cut(s)	2462, 3597
Csp6I	GTAC	9 cut(s)	149, 1068, 1163, 1467, 1508, 2005, 2081, 2265, 3046
CspCI	CAANNNNNGTGG	2 cut(s)	153, 188
CspI	CGGWCCG	1 cut(s)	3082
CviQI	GTAC	9 cut(s)	149, 1068, 1163, 1467, 1508, 2005, 2081, 2265, 3046
DdeI	CTNAG	7 cut(s)	1009, 2119, 2609, 2782, 3261, 3326, 3431
DraI	TTTAAA	1 cut(s)	1851
DraIII	CACNNNGTG	1 cut(s)	145
EaeI	YGGCCR	3 cut(s)	343, 400, 1707
Eam1104I	CTCTTC	2 cut(s)	898, 1479
EarI	CTCTTC	2 cut(s)	898, 1479
Eco130I	CCWWGG	6 cut(s)	279, 436, 824, 2000, 3019, 3303
Eco31I	GGTCTC	3 cut(s)	254, 446, 946
Eco47I	GGWCC	4 cut(s)	311, 828, 3082, 3515
Eco57I	CTGAAG	8 cut(s)	1192, 1283, 2273, 2382, 2686, 2690, 2849, 3252
Eco88I	CYCGRG	3 cut(s)	230, 486, 586
EcoO109I	RGGNCCY	1 cut(s)	3515
EcoRI	GAATTC	1 cut(s)	2786
EcoRII	CCWGG	2 cut(s)	504, 1693
EcoT14I	CCWWGG	6 cut(s)	279, 436, 824, 2000, 3019, 3303
EcoT22I	ATGCAT	2 cut(s)	2014, 2902
ErhI	CCWWGG	6 cut(s)	279, 436, 824, 2000, 3019, 3303
FaqI	GGGAC	4 cut(s)	443, 1027, 2304, 2465
FauI	CCCGC	2 cut(s)	2712, 3589
FbaI	TGATCA	4 cut(s)	1540, 1720, 2068, 2497
FblI	GTMKAC	3 cut(s)	97, 1596, 2675
Fnu4HI	GCNGC	8 cut(s)	132, 418, 798, 966, 3168, 3471, 3474, 3606
Fsp4HI	GCNGC	8 cut(s)	132, 418, 798, 966, 3168, 3471, 3474, 3606
FspBI	CTAG	6 cut(s)	837, 945, 1022, 1421, 2648, 2799
GlaI	GCGC	6 cut(s)	383, 416, 1563, 2052, 2687, 2689
GluI	GCNGC	8 cut(s)	132, 418, 798, 966, 3168, 3471, 3474, 3606
GsaI	CCCAGC	1 cut(s)	430
GsuI	CTGGAG	2 cut(s)	1092, 1848
HaeIII	GGCC	9 cut(s)	330, 345, 402, 722, 1569, 1709, 2762, 3018, 3157
HapII	CCGG	3 cut(s)	290, 928, 1327
HgaI	GACGC	2 cut(s)	2462, 3597
HhaI	GCGC	6 cut(s)	384, 417, 1564, 2053, 2688, 2690
Hin6I	GCGC	6 cut(s)	382, 415, 1562, 2051, 2686, 2688
HinP1I	GCGC	6 cut(s)	382, 415, 1562, 2051, 2686, 2688
HincII	GTYRAC	2 cut(s)	49, 3388
HindII	GTYRAC	2 cut(s)	49, 3388
HindIII	AAGCTT	1 cut(s)	3217
HpaII	CCGG	3 cut(s)	290, 928, 1327
HphI	GGTGA	7 cut(s)	794, 1112, 1511, 2561, 2924, 2935, 3123
Hpy166II	GTNNAC	9 cut(s)	49, 98, 1081, 1597, 2569, 2676, 3046, 3131, 3388
Hpy8I	GTNNAC	9 cut(s)	49, 98, 1081, 1597, 2569, 2676, 3046, 3131, 3388
Hpy99I	CGWCG	2 cut(s)	311, 1593
HpyCH4IV	ACGT	5 cut(s)	151, 1591, 2521, 2678, 3625
HpyF10VI	GCNNNNNNNGC	9 cut(s)	423, 432, 806, 1238, 2692, 2969, 3482, 3602, 3611
HpyF3I	CTNAG	7 cut(s)	1009, 2119, 2609, 2782, 3261, 3326, 3431
HpySE526I	ACGT	5 cut(s)	151, 1591, 2521, 2678, 3625
HspAI	GCGC	6 cut(s)	382, 415, 1562, 2051, 2686, 2688
Ksp22I	TGATCA	4 cut(s)	1540, 1720, 2068, 2497
LmnI	GCTCC	6 cut(s)	537, 962, 1093, 1333, 1672, 3292
Lsp1109I	GCAGC	7 cut(s)	118, 809, 952, 3154, 3457, 3485, 3592
MaeI	CTAG	6 cut(s)	837, 945, 1022, 1421, 2648, 2799
MaeII	ACGT	5 cut(s)	151, 1591, 2521, 2678, 3625
MaeIII	GTNAC	7 cut(s)	376, 409, 544, 577, 2522, 2766, 2806
MbiI	CCGCTC	1 cut(s)	3672
MfeI	CAATTG	2 cut(s)	266, 774
MflI	RGATCY	4 cut(s)	225, 1628, 3235, 3256
MhlI	GDGCHC	2 cut(s)	1090, 3297
MlsI	TGGCCA	2 cut(s)	345, 402
MluNI	TGGCCA	2 cut(s)	345, 402
MlyI	GAGTC	7 cut(s)	690, 1720, 2037, 2166, 2286, 3018, 3062
MmeI	TCCRAC	2 cut(s)	978, 3234
Mox20I	TGGCCA	2 cut(s)	345, 402
Mph1103I	ATGCAT	2 cut(s)	2014, 2902
MroXI	GAANNNNTTC	2 cut(s)	495, 2412
MscI	TGGCCA	2 cut(s)	345, 402
MslI	CAYNNNNRTG	4 cut(s)	738, 1748, 2183, 2574
Msp20I	TGGCCA	2 cut(s)	345, 402
MspI	CCGG	3 cut(s)	290, 928, 1327
MspR9I	CCNGG	4 cut(s)	506, 928, 1328, 1695
MunI	CAATTG	2 cut(s)	266, 774
Mva1269I	GAATGC	4 cut(s)	39, 110, 2412, 3371
MvaI	CCWGG	2 cut(s)	506, 1695
MvnI	CGCG	8 cut(s)	382, 415, 1116, 1564, 1731, 2517, 2686, 2688
MwoI	GCNNNNNNNGC	9 cut(s)	423, 432, 806, 1238, 2692, 2969, 3482, 3602, 3611
NciI	CCSGG	2 cut(s)	928, 1328
NcoI	CCATGG	2 cut(s)	436, 824
NmuCI	GTSAC	4 cut(s)	376, 409, 2522, 2766
NruI	TCGCGA	1 cut(s)	2517
NsiI	ATGCAT	2 cut(s)	2014, 2902
NspI	RCATGY	2 cut(s)	289, 3167
NspV	TTCGAA	1 cut(s)	1906
OliI	CACNNNNGTG	1 cut(s)	2183
PaeR7I	CTCGAG	1 cut(s)	230
PauI	GCGCGC	1 cut(s)	2686
PctI	GAATGC	4 cut(s)	39, 110, 2412, 3371
PdmI	GAANNNNTTC	2 cut(s)	495, 2412
PfeI	GAWTC	7 cut(s)	923, 1005, 1407, 1953, 2239, 2948, 3223
PflFI	GACNNNGTC	1 cut(s)	374
PflMI	CCANNNNNTGG	2 cut(s)	341, 352
PfoI	TCCNGGA	1 cut(s)	926
PkrI	GCNGC	8 cut(s)	133, 419, 799, 967, 3169, 3472, 3475, 3607
PleI	GAGTC	7 cut(s)	689, 1720, 2037, 2166, 2286, 3018, 3061
PpsI	GAGTC	7 cut(s)	689, 1720, 2037, 2166, 2286, 3018, 3061
Ppu21I	YACGTR	1 cut(s)	152
PpuMI	RGGWCCY	1 cut(s)	3515
PshBI	ATTAAT	1 cut(s)	3554
PsiI	TTATAA	1 cut(s)	618
Psp1406I	AACGTT	1 cut(s)	3625
Psp5II	RGGWCCY	1 cut(s)	3515
Psp6I	CCWGG	2 cut(s)	504, 1693
PspFI	CCCAGC	1 cut(s)	426
PspGI	CCWGG	2 cut(s)	504, 1693
PspPI	GGNCC	6 cut(s)	311, 828, 1568, 3016, 3082, 3515
PspPPI	RGGWCCY	1 cut(s)	3515
PspXI	VCTCGAGB	1 cut(s)	230
PstI	CTGCAG	2 cut(s)	2426, 3475
PstNI	CAGNNNCTG	1 cut(s)	2696
PsuI	RGATCY	4 cut(s)	225, 1628, 3235, 3256
PsyI	GACNNNGTC	1 cut(s)	374
PteI	GCGCGC	1 cut(s)	2686
RruI	TCGCGA	1 cut(s)	2517
RsaI	GTAC	9 cut(s)	150, 1069, 1164, 1468, 1509, 2006, 2082, 2266, 3047
RsaNI	GTAC	9 cut(s)	149, 1068, 1163, 1467, 1508, 2005, 2081, 2265, 3046
RseI	CAYNNNNRTG	4 cut(s)	738, 1748, 2183, 2574
Rsr2I	CGGWCCG	1 cut(s)	3082
RsrII	CGGWCCG	1 cut(s)	3082
SatI	GCNGC	8 cut(s)	132, 418, 798, 966, 3168, 3471, 3474, 3606
Sau96I	GGNCC	6 cut(s)	311, 828, 1568, 3016, 3082, 3515
ScaI	AGTACT	1 cut(s)	1509
SchI	GAGTC	7 cut(s)	690, 1720, 2037, 2166, 2286, 3018, 3062
ScrFI	CCNGG	4 cut(s)	506, 928, 1328, 1695
SduI	GDGCHC	2 cut(s)	1090, 3297
SfcI	CTRYAG	2 cut(s)	2422, 3471
Sfr274I	CTCGAG	1 cut(s)	230
SfuI	TTCGAA	1 cut(s)	1906
SinI	GGWCC	4 cut(s)	311, 828, 3082, 3515
SlaI	CTCGAG	1 cut(s)	230
SmiMI	CAYNNNNRTG	4 cut(s)	738, 1748, 2183, 2574
SmlI	CTYRAG	2 cut(s)	230, 2433
SmoI	CTYRAG	2 cut(s)	230, 2433
SpeI	ACTAGT	1 cut(s)	1021
SsiI	CCGC	9 cut(s)	418, 592, 1114, 1528, 1731, 2719, 3147, 3596, 3670
SspI	AATATT	3 cut(s)	169, 985, 2512
SspMI	CTAG	6 cut(s)	837, 945, 1022, 1421, 2648, 2799
StyD4I	CCNGG	4 cut(s)	504, 926, 1326, 1693
StyI	CCWWGG	6 cut(s)	279, 436, 824, 2000, 3019, 3303
TaiI	ACGT	5 cut(s)	154, 1594, 2524, 2681, 3628
TaqII	GACCGA	2 cut(s)	470, 3099
TatI	WGTACW	3 cut(s)	1507, 2080, 3045
TauI	GCSGC	1 cut(s)	420
TfiI	GAWTC	7 cut(s)	923, 1005, 1407, 1953, 2239, 2948, 3223
TseFI	GTSAC	4 cut(s)	376, 409, 2522, 2766
TseI	GCWGC	7 cut(s)	131, 797, 965, 3167, 3470, 3473, 3605
Tsp45I	GTSAC	4 cut(s)	376, 409, 2522, 2766
TspGWI	ACGGA	4 cut(s)	641, 777, 3182, 3691
Tth111I	GACNNNGTC	1 cut(s)	374
Van91I	CCANNNNNTGG	2 cut(s)	341, 352
VpaK11BI	GGWCC	4 cut(s)	311, 828, 3082, 3515
VspI	ATTAAT	1 cut(s)	3554
XapI	RAATTY	6 cut(s)	865, 1574, 2786, 3187, 3265, 3417
XbaI	TCTAGA	1 cut(s)	2647
XceI	RCATGY	2 cut(s)	289, 3167
XcmI	CCANNNNNNNNNTGG	2 cut(s)	353, 395
XhoI	CTCGAG	1 cut(s)	230
XmiI	GTMKAC	3 cut(s)	97, 1596, 2675
XmnI	GAANNNNTTC	2 cut(s)	495, 2412
XspI	CTAG	6 cut(s)	837, 945, 1022, 1421, 2648, 2799
ZrmI	AGTACT	1 cut(s)	1509
Zsp2I	ATGCAT	2 cut(s)	2014, 2902

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.