Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0009542

Physical Location & Seq

Genomic Coordinates Reverse (-)

37554016 .. 37570189

Gene Structure 16174 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG148700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3270 bp

ATGGAAGAGGTATGCGAGGGCAAGGACTTCTCCTTCCCGAAGCAAGAGGAGAAGATTCTCCACTACTGGTCCCAAATCAAAGCCTTCGAGACTCAATTGTCTCTCACCGAACACATGCCCGAGTACGTCTTCTACGACGGCCCCCCCTTCGCCACCGGCCTCCCTCACTACGGCCACATACTGGCCGGCACCATCAAGGACATCATCACGCGCTACCAGTCCATGACCGGCCACCACGTCACGCGCCGCTTCGGCTGGGACTGCCACGGCCTTCCCGTCGAGAACGAGATTGATAAGAAGCTCGACATCAGGCGCCGGGAACAGGTTCTCGAAATGGGGATCGATAAGTACAACGAGGAGTGCCGGAGCATTGTTACTCGGTTTGTGGAGGAGTGGGAGAAGGTTATTGCGCGCACTGGGAGGTGGATCGATTTTCGGAACGATTATAAGACCATGGACTTGAAGTTTATGGAGAGTGTGTGGTGGGTTTTTGCTCAGATATATGATAAGGGCCTTGTCTATAAGGGCTTCAAGGTCATGCCGTACAGCACCGGTTGCAAGACACCGCTTTCGAATTTCGAGGCTAATCAAGATTACAAGGATGTTCCCGATCCAGAAGTTATGGTGGCTTTTCCAATTATTGGTGATTCTGACGAGGCAAGTTTTGTGGCGTGGACAACTACTCCGTGGACCCTTCCTAGCCATTTAGCGCTTTGTGTCAATGCAAATTTTACCTATTTGAAGGTTTGCAACAAGTACTCAAAAAAAGTTTACGTCGTTGCTGAATCTCGATTGTCAGCTCTTCCTAGTGACAAACCAAAAGAAAACGTACCAAATGGATCTGTTGATTCAAAAAAATTAAATTCAAAGAGCAAGGGATCCTCGGGTGGGAAAAAGGAAGCAGTTGACACATCATATGAAGTGTTGGAGAAAATGTCCGGGGCTTCATTAGTGGGGAAGAAGTATGAACCACCATTTGATTACTTTAAGGAGTTCTCTGATGTTGCATTTAGAGTTGTTGCGGACAACTATGTTACTGATGACAGTGGTACTGGAATTGTCCACTGTGCTCCTGCATTTGGTGAAGACGATTATCGTGTTTGCCTTGAAAATAAAGTTATCAGTAAGGGAGAGACCTTGATTGTAGCAGTTGATGAGGATGGATGCTTTACTGAAAAAATTACCGACTTCAGCAAGTGCTATGTCAAAAATGCAGATAAAGATATTATTGAAGCATTGAAGCGGAAAGGAAGGCTTGTTAAGTCTGGGACCATTATGCATTCTTATCCCCATTGCCCAAGATCTAAAACTCCACTTATTCAAAGAGCTGTTCCAAGTTGGTTTATCCGGGTGGAGCAGTTGAAAGAAAAATTGTTAGAAAACAACAAGCAGACATATTGGGTTCCTGATTTTGTGAAGGAAAAACGATTCCACAATTGGCTGGAAAATGCAAGAGATTGGGCAGTTAGTCGAAGTAGATTCTGGGGCACTCCTCTTCCTGTGTGGATTAGTGAGGATGGGGAGGAAATAGTAGTTATGGATTCCATTGAGAAGCTCGAAAAGCTTTCTGGTGTTAAGGTTTTTGATCTTCACCGGCACAATATCGATCATATCACTGTTCCATCTAGCCGTGGTCCTCAATTTGGCGTTCTTCGACGTATTGATGATGTGTTTGATTGTTGGTTTGAGAGTGGTTCCATGCCTTATGCTTATATCCACTATCCTTTTGAGAATGTTGAACTTTTTGAGAAAAACTTTCCTGGAAATTTTGTGGCTGAAGGGCTGGATCAGACTCGTGGATGGTTCTACACTCTTATGGTGTTATCTACCGCATTATTTGGGAAACCTGCTTTTAAGAATCTCATTTGCAATGGACTCGTACTTGCTGAGGATGGAAAAAAAATGAGTAAAAGTCTGAAAAATTATCCTCCACCAGTTGAAGTTATCGATCAGTATGGAGCTGATGCAGTACGATTATACCTAATTAACTCTCCTGTTGTGCGCGCGGAACCATTACGTTTCAAGAAGGAAGGAGTTTATGGTGTTGTCAAGGATGTATTTCTTCCATGGTACAATGCATATAGGTTCCTTGTCCAGAATGCAAAGAGACTTGAGATTGAAGGGTTTGCCCCTTTTGTTCCTATTGATCAAGCCACTCTACAAAAGTCATCTAATGTGCTTGACCAGTGGATCTACTCAGCCACACAGAGTCTTGTGTATTTTGTCCGTCAGGAGATGAACGGTTATCGACTTTATACGGTGGTTCCATATCTTTTGAAGTTTCTAGACAACTTGACTAATATCTATGTGAGGTGCAATCGCAAGAGGTTGAAGGGTCGTACAGGTGAAGAAGATTGTAGGGTGGCGCTCTCAACACTTTACAATGTCCTTTTGGTATCATGTAAAGCGATGGCTCCATTAACACCATTTTTCACTGAGGTCCTTTTTCAAAATATGCGAAAAGTTTCCAACACGGCAGAGGAAAGCATTCATCATTGCAGCTTTCCTGAAGCAGAAGGCAAGAGGGATGAACGTATTGAGCAAAGTGTTGCAAGGATGATGACAATAATTGATCTTGCCCGTAATATTCGTGAGAGACGTAACAAACCTCTTAAAACACCACTGAGGGAGATGGTTATCGTTCATCCTGATGTGGACTTTCTTGATGATATCGCTGGAAGGCTAAAAGAGTATGTGCTGGAGGAACTTAATGTGAGATCTCTTGTTCCATGTAATGATACACTGAAGTATGCTTCATTACGTGCTGAGCCTGATTTCAGCGTTTTGGGTAAGCGGCTAGGAAAACATATGGGAATTGTTGCCAAAGAAGTCAAGGCAATGTCACAGGAAAGCATTCTAGCATTTGAGAAATCCGGAGAAGTTACTCTTTCTGGTCATTGCTTAAAGCTGACTGATATTAAGGTTGTTAGGGATTTCAAGTGTCCTGATGGCACTGCAGTAGACGACGTGGATGCTACAGGAGATGGTGATGTTTTGGTGATTTTGGATTTACGTTCCGATGAGTCGTTGGCTGATGCGGGTGTTGCTCGAGAGATTGTCAACAGAATCCAGAAGTTAAGAAAGAAATCTGCTCTTGAACCTACTGACTCGGTGGAGGTCTACTTTGACTCTTTGGATGAAGATAAAGCAGATTCCCAAAGGATCTTGCAATCACAGGAACAATACATTAGGGATGCCATTGGTTCGCCATTGCTTCCTTCAAGTGTAATGCCATCACATGCTGTAAGTTCTATTGTTATTAGGAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004822 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006428 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0017101 GO:0019538 GO:0019752 GO:0019899 GO:0032991 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0051020 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1

Protein Analysis

1089

Amino Acids

124.41

Weight (kDa)

6.2

Isoelectric Point (pI)

43.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	19 - 670	3.3e-157	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g	PF09334	49 - 187	9.6e-06	tRNA synthetases class I (M)
Anticodon_1	PF08264	728 - 877	3.4e-26	Anticodon-binding domain of tRNA ligase
DUF5915	PF19302	897 - 1064	1.4e-25	Domain of unknown function (DUF5915)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0009542)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G10320
fragaria_vesca	FvH4_4g11470
malus_domestica	MD04G1037200.v1.1 MD09G1065200.v1.1
prunus_persica	Prupe.1G186600_v2.0.a1 Prupe.1G186600_v2.0.a1
pyrus_communis	pycom04g03030 pycom111g05510
rosa_chinensis	RchiOBHm_Chr4g0408181
rosa_laevigata	RLG00000008666 RLG00000035346
rosa_multiflora	Rmu_ssc0000039.1_g000001
rosa_roxburghii	Rroxscaffold_1G00020510 Rroxscaffold_5G00352680
rosa_rugosa	Rorug04G0083900 Rorug04G0084000 Rorug05G0328300
rosa_samantha	Rh4AG148700 Rh4BG145500 Rh4CG155200 Rh4DG142000
rosa_wichuraiana	Rw4G012140

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	447
AasI	GACNNNNNNGTC	1 cut(s)	97
Acc36I	ACCTGC	1 cut(s)	1855
AccB1I	GGYRCC	2 cut(s)	188, 312
AccB7I	CCANNNNNTGG	2 cut(s)	181, 641
AccI	GTMKAC	2 cut(s)	2961, 3120
AccII	CGCG	5 cut(s)	211, 244, 412, 2004, 2006
AccIII	TCCGGA	1 cut(s)	2873
AciI	CCGC	8 cut(s)	247, 566, 1022, 1243, 1830, 2006, 2794, 3038
AclWI	GGATC	9 cut(s)	347, 434, 605, 847, 873, 886, 1794, 2198, 3170
AcoI	YGGCCR	3 cut(s)	172, 183, 229
AcsI	RAATTY	4 cut(s)	574, 727, 862, 1765
AcuI	CTGAAG	4 cut(s)	1174, 1797, 2529, 2765
AcyI	GRCGYC	1 cut(s)	313
AfeI	AGCGCT	1 cut(s)	711
AfiI	CCNNNNNNNGG	7 cut(s)	170, 181, 322, 641, 888, 1079, 1643
AgeI	ACCGGT	1 cut(s)	551
AjiI	CACGTC	2 cut(s)	238, 2968
AjnI	CCWGG	1 cut(s)	1759
AluBI	AGCT	8 cut(s)	301, 800, 1328, 1555, 1564, 1961, 2502, 2908
AluI	AGCT	8 cut(s)	301, 800, 1328, 1555, 1564, 1961, 2502, 2908
Alw21I	GWGCWC	1 cut(s)	1072
Alw26I	GTCTC	5 cut(s)	83, 105, 1127, 2101, 2590
AlwI	GGATC	9 cut(s)	347, 434, 605, 847, 873, 886, 1794, 2198, 3170
Ama87I	CYCGRG	3 cut(s)	119, 883, 3048
Aor13HI	TCCGGA	1 cut(s)	2873
Aor51HI	AGCGCT	1 cut(s)	711
AoxI	GGCC	7 cut(s)	139, 157, 172, 183, 229, 268, 511
ApeKI	GCWGC	1 cut(s)	2499
ApoI	RAATTY	4 cut(s)	574, 727, 862, 1765
ArsI	GACNNNNNNTTYG	2 cut(s)	553, 585
AsiGI	ACCGGT	1 cut(s)	551
Asp700I	GAANNNNTTC	4 cut(s)	324, 2464, 2487, 2853
AspLEI	GCGC	9 cut(s)	213, 246, 315, 412, 414, 712, 2004, 2006, 2368
AspS9I	GGNCC	7 cut(s)	69, 140, 511, 690, 1269, 1634, 2440
AsuC2I	CCSGG	3 cut(s)	317, 940, 1349
AsuHPI	GGTGA	7 cut(s)	97, 656, 1094, 1583, 2357, 2999, 3010
AsuII	TTCGAA	1 cut(s)	572
AvaI	CYCGRG	3 cut(s)	119, 883, 3048
AvaII	GGWCC	5 cut(s)	69, 690, 1269, 1634, 2440
BaeGI	GKGCMC	1 cut(s)	1490
BamHI	GGATCC	1 cut(s)	878
BanI	GGYRCC	2 cut(s)	188, 312
BauI	CACGAG	1 cut(s)	1794
BbsI	GAAGAC	2 cut(s)	121, 1092
Bbv12I	GWGCWC	1 cut(s)	1072
BbvCI	CCTCAGC	1 cut(s)	1887
BbvI	GCAGC	1 cut(s)	2511
BceAI	ACGGC	6 cut(s)	154, 187, 283, 526, 1614, 2490
BcgI	CGANNNNNNTGC	4 cut(s)	1858, 1892, 2954, 2988
BciT130I	CCWGG	1 cut(s)	1761
BclI	TGATCA	1 cut(s)	2146
BcnI	CCSGG	3 cut(s)	317, 940, 1349
BcoDI	GTCTC	5 cut(s)	83, 105, 1127, 2101, 2590
BfaI	CTAG	6 cut(s)	699, 807, 1626, 2285, 2798, 2858
BfmI	CTRYAG	2 cut(s)	2955, 2976
BfoI	RGCGCY	3 cut(s)	316, 713, 2369
BfuAI	ACCTGC	1 cut(s)	1855
BglI	GCCNNNNNGGC	1 cut(s)	252
BglII	AGATCT	2 cut(s)	1301, 2717
BisI	GCNGC	3 cut(s)	247, 2500, 2795
BlpI	GCTNAGC	1 cut(s)	2766
BlsI	GCNGC	3 cut(s)	248, 2501, 2796
BmcAI	AGTACT	1 cut(s)	758
Bme1390I	CCNGG	4 cut(s)	317, 940, 1349, 1761
Bme18I	GGWCC	5 cut(s)	69, 690, 1269, 1634, 2440
BmeT110I	CYCGRG	3 cut(s)	119, 883, 3048
BmgBI	CACGTC	2 cut(s)	238, 2968
BmgT120I	GGNCC	7 cut(s)	69, 140, 511, 690, 1269, 1634, 2440
BmrFI	CCNGG	4 cut(s)	317, 940, 1349, 1761
BmrI	ACTGGG	1 cut(s)	426
BmsI	GCATC	5 cut(s)	1154, 1954, 2962, 3025, 3184
BmuI	ACTGGG	1 cut(s)	426
BpiI	GAAGAC	2 cut(s)	121, 1092
BpmI	CTGGAG	1 cut(s)	2720
Bpu10I	CCTNAGC	1 cut(s)	1887
Bpu1102I	GCTNAGC	1 cut(s)	2766
Bpu14I	TTCGAA	1 cut(s)	572
BpuEI	CTTGAG	1 cut(s)	2132
BpuMI	CCSGG	3 cut(s)	317, 940, 1349
Bsa29I	ATCGAT	4 cut(s)	342, 429, 1605, 1947
BsaAI	YACGTR	1 cut(s)	2762
BsaBI	GATNNNNATC	1 cut(s)	2636
BsaHI	GRCGYC	1 cut(s)	313
BsaI	GGTCTC	1 cut(s)	1127
BsaJI	CCNNGG	7 cut(s)	265, 453, 686, 882, 939, 1630, 2066
BsaWI	WCCGGW	2 cut(s)	551, 2873
BsaXI	ACNNNNNCTCC	6 cut(s)	464, 494, 667, 697, 920, 950
Bsc4I	CCNNNNNNNGG	7 cut(s)	170, 181, 322, 641, 888, 1079, 1643
Bse118I	RCCGGY	5 cut(s)	155, 185, 227, 551, 1593
Bse1I	ACTGG	7 cut(s)	71, 186, 217, 421, 1057, 1934, 2185
Bse3DI	GCAATG	6 cut(s)	1291, 1876, 2494, 2844, 2896, 3209
Bse8I	GATNNNNATC	1 cut(s)	2636
BseAI	TCCGGA	1 cut(s)	2873
BseBI	CCWGG	1 cut(s)	1761
BseCI	ATCGAT	4 cut(s)	342, 429, 1605, 1947
BseDI	CCNNGG	7 cut(s)	265, 453, 686, 882, 939, 1630, 2066
BseJI	GATNNNNATC	1 cut(s)	2636
BseLI	CCNNNNNNNGG	7 cut(s)	170, 181, 322, 641, 888, 1079, 1643
BseMI	GCAATG	6 cut(s)	1291, 1876, 2494, 2844, 2896, 3209
BseMII	CTCAG	6 cut(s)	509, 1878, 2211, 2427, 2615, 2757
BseNI	ACTGG	7 cut(s)	71, 186, 217, 421, 1057, 1934, 2185
BsePI	GCGCGC	2 cut(s)	410, 2002
BseRI	GAGGAG	4 cut(s)	62, 371, 404, 1482
BseSI	GKGCMC	1 cut(s)	1490
BseXI	GCAGC	1 cut(s)	2511
BseYI	CCCAGC	1 cut(s)	255
Bsh1236I	CGCG	5 cut(s)	211, 244, 412, 2004, 2006
BshFI	GGCC	7 cut(s)	141, 159, 174, 185, 231, 270, 513
BshNI	GGYRCC	2 cut(s)	188, 312
BshTI	ACCGGT	1 cut(s)	551
BshVI	ATCGAT	4 cut(s)	342, 429, 1605, 1947
BsiHKAI	GWGCWC	1 cut(s)	1072
BsiHKCI	CYCGRG	3 cut(s)	119, 883, 3048
BslFI	GGGAC	3 cut(s)	55, 272, 1282
BslI	CCNNNNNNNGG	7 cut(s)	170, 181, 322, 641, 888, 1079, 1643
BsmAI	GTCTC	5 cut(s)	83, 105, 1127, 2101, 2590
BsmBI	CGTCTC	1 cut(s)	2590
BsmFI	GGGAC	3 cut(s)	55, 272, 1282
BsmI	GAATGC	4 cut(s)	1279, 2104, 2487, 2853
BsnI	GGCC	7 cut(s)	141, 159, 174, 185, 231, 270, 513
Bso31I	GGTCTC	1 cut(s)	1127
BsoBI	CYCGRG	3 cut(s)	119, 883, 3048
Bsp119I	TTCGAA	1 cut(s)	572
Bsp1286I	GDGCHC	2 cut(s)	1072, 1490
Bsp13I	TCCGGA	1 cut(s)	2873
Bsp1720I	GCTNAGC	1 cut(s)	2766
Bsp19I	CCATGG	2 cut(s)	453, 2066
BspACI	CCGC	8 cut(s)	247, 566, 1022, 1243, 1830, 2006, 2794, 3038
BspANI	GGCC	7 cut(s)	141, 159, 174, 185, 231, 270, 513
BspCNI	CTCAG	6 cut(s)	508, 1879, 2210, 2428, 2616, 2758
BspDI	ATCGAT	4 cut(s)	342, 429, 1605, 1947
BspEI	TCCGGA	1 cut(s)	2873
BspFNI	CGCG	5 cut(s)	211, 244, 412, 2004, 2006
BspMAI	CTGCAG	1 cut(s)	2959
BspMI	ACCTGC	1 cut(s)	1855
BspPI	GGATC	9 cut(s)	347, 434, 605, 847, 873, 886, 1794, 2198, 3170
BspQI	GCTCTTC	1 cut(s)	807
BspT104I	TTCGAA	1 cut(s)	572
BspT107I	GGYRCC	2 cut(s)	188, 312
BspTNI	GGTCTC	1 cut(s)	1127
BsrDI	GCAATG	6 cut(s)	1291, 1876, 2494, 2844, 2896, 3209
BsrFI	RCCGGY	5 cut(s)	155, 185, 227, 551, 1593
BsrI	ACTGG	7 cut(s)	71, 186, 217, 421, 1057, 1934, 2185
BssAI	RCCGGY	5 cut(s)	155, 185, 227, 551, 1593
BssECI	CCNNGG	7 cut(s)	265, 453, 686, 882, 939, 1630, 2066
BssHII	GCGCGC	2 cut(s)	410, 2002
BssNI	GRCGYC	1 cut(s)	313
BssSI	CACGAG	1 cut(s)	1794
BssT1I	CCWWGG	2 cut(s)	453, 2066
Bst2BI	CACGAG	1 cut(s)	1794
Bst2UI	CCWGG	1 cut(s)	1761
Bst4CI	ACNGT	5 cut(s)	1046, 1067, 1618, 2243, 2260
Bst6I	CTCTTC	2 cut(s)	807, 1500
BstACI	GRCGYC	1 cut(s)	313
BstAPI	GCANNNNNTGC	1 cut(s)	555
BstBAI	YACGTR	1 cut(s)	2762
BstBI	TTCGAA	1 cut(s)	572
BstC8I	GCNNGC	3 cut(s)	187, 412, 2004
BstDEI	CTNAG	6 cut(s)	495, 1887, 2197, 2436, 2624, 2766
BstDSI	CCRYGG	5 cut(s)	265, 453, 686, 1630, 2066
BstFNI	CGCG	5 cut(s)	211, 244, 412, 2004, 2006
BstH2I	RGCGCY	3 cut(s)	316, 713, 2369
BstHHI	GCGC	9 cut(s)	213, 246, 315, 412, 414, 712, 2004, 2006, 2368
BstMAI	GTCTC	5 cut(s)	83, 105, 1127, 2101, 2590
BstMWI	GCNNNNNNNGC	5 cut(s)	252, 261, 555, 1561, 3044
BstNI	CCWGG	1 cut(s)	1761
BstNSI	RCATGY	2 cut(s)	118, 3242
BstSCI	CCNGG	4 cut(s)	315, 938, 1347, 1759
BstSFI	CTRYAG	2 cut(s)	2955, 2976
BstSLI	GKGCMC	1 cut(s)	1490
BstUI	CGCG	5 cut(s)	211, 244, 412, 2004, 2006
BstV1I	GCAGC	1 cut(s)	2511
BstV2I	GAAGAC	2 cut(s)	121, 1092
BstX2I	RGATCY	6 cut(s)	839, 878, 1301, 2190, 2717, 3162
BstYI	RGATCY	6 cut(s)	839, 878, 1301, 2190, 2717, 3162
Bsu15I	ATCGAT	4 cut(s)	342, 429, 1605, 1947
BsuRI	GGCC	7 cut(s)	141, 159, 174, 185, 231, 270, 513
BsuTUI	ATCGAT	4 cut(s)	342, 429, 1605, 1947
BtgI	CCRYGG	5 cut(s)	265, 453, 686, 1630, 2066
BtgZI	GCGATG	1 cut(s)	2423
BtrI	CACGTC	2 cut(s)	238, 2968
BtsI	GCAGTG	1 cut(s)	2952
BtsIMutI	CAGTG	9 cut(s)	414, 1051, 1063, 1614, 2192, 2433, 2621, 2741, 2952
BveI	ACCTGC	1 cut(s)	1855
Cac8I	GCNNGC	3 cut(s)	187, 412, 2004
CfoI	GCGC	9 cut(s)	213, 246, 315, 412, 414, 712, 2004, 2006, 2368
Cfr10I	RCCGGY	5 cut(s)	155, 185, 227, 551, 1593
Cfr13I	GGNCC	7 cut(s)	69, 140, 511, 690, 1269, 1634, 2440
ClaI	ATCGAT	4 cut(s)	342, 429, 1605, 1947
CspAI	ACCGGT	1 cut(s)	551
CspCI	CAANNNNNGTGG	2 cut(s)	648, 683
CviAII	CATG	9 cut(s)	115, 223, 454, 538, 1699, 2067, 2400, 2730, 3239
DdeI	CTNAG	6 cut(s)	495, 1887, 2197, 2436, 2624, 2766
DinI	GGCGCC	1 cut(s)	314
DrdI	GACNNNNNNGTC	1 cut(s)	97
DseDI	GACNNNNNNGTC	1 cut(s)	97
EaeI	YGGCCR	3 cut(s)	172, 183, 229
Eam1104I	CTCTTC	2 cut(s)	807, 1500
EarI	CTCTTC	2 cut(s)	807, 1500
Eco130I	CCWWGG	2 cut(s)	453, 2066
Eco31I	GGTCTC	1 cut(s)	1127
Eco32I	GATATC	1 cut(s)	2671
Eco47I	GGWCC	5 cut(s)	69, 690, 1269, 1634, 2440
Eco47III	AGCGCT	1 cut(s)	711
Eco57I	CTGAAG	4 cut(s)	1174, 1797, 2529, 2765
Eco88I	CYCGRG	3 cut(s)	119, 883, 3048
EcoO109I	RGGNCCY	2 cut(s)	511, 2440
EcoRII	CCWGG	1 cut(s)	1759
EcoRV	GATATC	1 cut(s)	2671
EcoT14I	CCWWGG	2 cut(s)	453, 2066
EcoT22I	ATGCAT	2 cut(s)	1281, 2080
EgeI	GGCGCC	1 cut(s)	314
EheI	GGCGCC	1 cut(s)	314
ErhI	CCWWGG	2 cut(s)	453, 2066
Esp3I	CGTCTC	1 cut(s)	2590
FaeI	CATG	9 cut(s)	118, 226, 457, 541, 1702, 2070, 2403, 2733, 3242
FalI	AAGNNNNNCTT	4 cut(s)	1239, 1271, 2871, 2903
FaqI	GGGAC	3 cut(s)	55, 272, 1282
FatI	CATG	9 cut(s)	114, 222, 453, 537, 1698, 2066, 2399, 2729, 3238
FauI	CCCGC	1 cut(s)	3031
FauNDI	CATATG	2 cut(s)	916, 2808
FbaI	TGATCA	1 cut(s)	2146
FblI	GTMKAC	2 cut(s)	2961, 3120
Fnu4HI	GCNGC	3 cut(s)	247, 2500, 2795
Fsp4HI	GCNGC	3 cut(s)	247, 2500, 2795
FspBI	CTAG	6 cut(s)	699, 807, 1626, 2285, 2798, 2858
GlaI	GCGC	9 cut(s)	212, 245, 314, 411, 413, 711, 2003, 2005, 2367
GluI	GCNGC	3 cut(s)	247, 2500, 2795
GsaI	CCCAGC	1 cut(s)	259
GsuI	CTGGAG	1 cut(s)	2720
HaeII	RGCGCY	3 cut(s)	316, 713, 2369
HaeIII	GGCC	7 cut(s)	141, 159, 174, 185, 231, 270, 513
HhaI	GCGC	9 cut(s)	213, 246, 315, 412, 414, 712, 2004, 2006, 2368
Hin1I	GRCGYC	1 cut(s)	313
Hin1II	CATG	9 cut(s)	118, 226, 457, 541, 1702, 2070, 2403, 2733, 3242
Hin6I	GCGC	9 cut(s)	211, 244, 313, 410, 412, 710, 2002, 2004, 2366
HinP1I	GCGC	9 cut(s)	211, 244, 313, 410, 412, 710, 2002, 2004, 2366
HincII	GTYRAC	2 cut(s)	907, 3061
HindII	GTYRAC	2 cut(s)	907, 3061
HindIII	AAGCTT	1 cut(s)	1562
HphI	GGTGA	7 cut(s)	97, 656, 1094, 1583, 2357, 2999, 3010
Hpy166II	GTNNAC	9 cut(s)	675, 690, 772, 907, 1063, 2656, 2962, 3061, 3121
Hpy188I	TCNGA	7 cut(s)	438, 498, 652, 1000, 1791, 1917, 3019
Hpy8I	GTNNAC	9 cut(s)	675, 690, 772, 907, 1063, 2656, 2962, 3061, 3121
Hpy99I	CGWCG	5 cut(s)	140, 281, 779, 1659, 2969
HpyCH4III	ACNGT	5 cut(s)	1046, 1067, 1618, 2243, 2260
HpyF10VI	GCNNNNNNNGC	5 cut(s)	252, 261, 555, 1561, 3044
HpyF3I	CTNAG	6 cut(s)	495, 1887, 2197, 2436, 2624, 2766
Hsp92I	GRCGYC	1 cut(s)	313
Hsp92II	CATG	9 cut(s)	118, 226, 457, 541, 1702, 2070, 2403, 2733, 3242
HspAI	GCGC	9 cut(s)	211, 244, 313, 410, 412, 710, 2002, 2004, 2366
KasI	GGCGCC	1 cut(s)	312
Kpn2I	TCCGGA	1 cut(s)	2873
KroI	GCCGGC	1 cut(s)	185
KroNI	GCCGGC	1 cut(s)	187
Ksp22I	TGATCA	1 cut(s)	2146
LguI	GCTCTTC	1 cut(s)	807
LmnI	GCTCC	5 cut(s)	366, 1075, 1354, 1958, 2419
Lsp1109I	GCAGC	1 cut(s)	2511
LweI	GCATC	5 cut(s)	1154, 1954, 2962, 3025, 3184
MaeI	CTAG	6 cut(s)	699, 807, 1626, 2285, 2798, 2858
MaeIII	GTNAC	7 cut(s)	238, 373, 809, 1033, 2600, 2841, 2881
MfeI	CAATTG	2 cut(s)	95, 1435
MflI	RGATCY	6 cut(s)	839, 878, 1301, 2190, 2717, 3162
MhlI	GDGCHC	2 cut(s)	1072, 1490
Mly113I	GGCGCC	1 cut(s)	313
MlyI	GAGTC	7 cut(s)	85, 1786, 1869, 2218, 3032, 3101, 3122
MmeI	TCCRAC	2 cut(s)	906, 2493
Mph1103I	ATGCAT	2 cut(s)	1281, 2080
MroI	TCCGGA	1 cut(s)	2873
MroNI	GCCGGC	1 cut(s)	185
MroXI	GAANNNNTTC	4 cut(s)	324, 2464, 2487, 2853
MslI	CAYNNNNRTG	2 cut(s)	1814, 2649
MspR9I	CCNGG	4 cut(s)	317, 940, 1349, 1761
MunI	CAATTG	2 cut(s)	95, 1435
Mva1269I	GAATGC	4 cut(s)	1279, 2104, 2487, 2853
MvaI	CCWGG	1 cut(s)	1761
MvnI	CGCG	5 cut(s)	211, 244, 412, 2004, 2006
MwoI	GCNNNNNNNGC	5 cut(s)	252, 261, 555, 1561, 3044
NaeI	GCCGGC	1 cut(s)	187
NarI	GGCGCC	1 cut(s)	313
NciI	CCSGG	3 cut(s)	317, 940, 1349
NcoI	CCATGG	2 cut(s)	453, 2066
NdeI	CATATG	2 cut(s)	916, 2808
NgoMIV	GCCGGC	1 cut(s)	185
NlaIII	CATG	9 cut(s)	118, 226, 457, 541, 1702, 2070, 2403, 2733, 3242
NmuCI	GTSAC	3 cut(s)	238, 809, 2841
NsiI	ATGCAT	2 cut(s)	1281, 2080
NspI	RCATGY	2 cut(s)	118, 3242
NspV	TTCGAA	1 cut(s)	572
PaeR7I	CTCGAG	1 cut(s)	3048
PauI	GCGCGC	2 cut(s)	410, 2002
PciSI	GCTCTTC	1 cut(s)	807
PcsI	WCGNNNNNNNCGW	3 cut(s)	132, 273, 2596
PctI	GAATGC	4 cut(s)	1279, 2104, 2487, 2853
PdiI	GCCGGC	1 cut(s)	187
PdmI	GAANNNNTTC	4 cut(s)	324, 2464, 2487, 2853
PflMI	CCANNNNNTGG	2 cut(s)	181, 641
PfoI	TCCNGGA	1 cut(s)	1759
PinAI	ACCGGT	1 cut(s)	551
PkrI	GCNGC	3 cut(s)	248, 2501, 2796
PleI	GAGTC	7 cut(s)	85, 1786, 1869, 2217, 3031, 3101, 3122
PluTI	GGCGCC	1 cut(s)	316
PpsI	GAGTC	7 cut(s)	85, 1786, 1869, 2217, 3031, 3101, 3122
Ppu21I	YACGTR	1 cut(s)	2762
PpuMI	RGGWCCY	1 cut(s)	2440
PsiI	TTATAA	1 cut(s)	447
Psp5II	RGGWCCY	1 cut(s)	2440
Psp6I	CCWGG	1 cut(s)	1759
PspFI	CCCAGC	1 cut(s)	255
PspGI	CCWGG	1 cut(s)	1759
PspPI	GGNCC	7 cut(s)	69, 140, 511, 690, 1269, 1634, 2440
PspPPI	RGGWCCY	1 cut(s)	2440
PstI	CTGCAG	1 cut(s)	2959
PsuI	RGATCY	6 cut(s)	839, 878, 1301, 2190, 2717, 3162
PteI	GCGCGC	2 cut(s)	410, 2002
RseI	CAYNNNNRTG	2 cut(s)	1814, 2649
SapI	GCTCTTC	1 cut(s)	807
SatI	GCNGC	3 cut(s)	247, 2500, 2795
Sau96I	GGNCC	7 cut(s)	69, 140, 511, 690, 1269, 1634, 2440
ScaI	AGTACT	1 cut(s)	758
SchI	GAGTC	7 cut(s)	85, 1786, 1869, 2218, 3032, 3101, 3122
ScrFI	CCNGG	4 cut(s)	317, 940, 1349, 1761
SduI	GDGCHC	2 cut(s)	1072, 1490
SfaNI	GCATC	5 cut(s)	1154, 1954, 2962, 3025, 3184
SfcI	CTRYAG	2 cut(s)	2955, 2976
SfoI	GGCGCC	1 cut(s)	314
Sfr274I	CTCGAG	1 cut(s)	3048
SfuI	TTCGAA	1 cut(s)	572
SinI	GGWCC	5 cut(s)	69, 690, 1269, 1634, 2440
SlaI	CTCGAG	1 cut(s)	3048
SmiMI	CAYNNNNRTG	2 cut(s)	1814, 2649
SmlI	CTYRAG	2 cut(s)	2111, 3048
SmoI	CTYRAG	2 cut(s)	2111, 3048
SsiI	CCGC	8 cut(s)	247, 566, 1022, 1243, 1830, 2006, 2794, 3038
SspDI	GGCGCC	1 cut(s)	312
SspI	AATATT	1 cut(s)	2587
SspMI	CTAG	6 cut(s)	699, 807, 1626, 2285, 2798, 2858
StyD4I	CCNGG	4 cut(s)	315, 938, 1347, 1759
StyI	CCWWGG	2 cut(s)	453, 2066
TaaI	ACNGT	5 cut(s)	1046, 1067, 1618, 2243, 2260
TatI	WGTACW	2 cut(s)	348, 756
TauI	GCSGC	2 cut(s)	249, 2797
TscAI	CASTG	9 cut(s)	421, 1051, 1070, 1621, 2192, 2440, 2628, 2748, 2959
TseFI	GTSAC	3 cut(s)	238, 809, 2841
TseI	GCWGC	1 cut(s)	2499
Tsp45I	GTSAC	3 cut(s)	238, 809, 2841
TspDTI	ATGAA	9 cut(s)	933, 936, 981, 2252, 2480, 2544, 2633, 2745, 3153
TspGWI	ACGGA	2 cut(s)	675, 2216
TspRI	CASTG	9 cut(s)	421, 1051, 1070, 1621, 2192, 2440, 2628, 2748, 2959
Van91I	CCANNNNNTGG	2 cut(s)	181, 641
VpaK11BI	GGWCC	5 cut(s)	69, 690, 1269, 1634, 2440
XapI	RAATTY	4 cut(s)	574, 727, 862, 1765
XbaI	TCTAGA	1 cut(s)	2284
XceI	RCATGY	2 cut(s)	118, 3242
XhoI	CTCGAG	1 cut(s)	3048
XmiI	GTMKAC	2 cut(s)	2961, 3120
XmnI	GAANNNNTTC	4 cut(s)	324, 2464, 2487, 2853
XspI	CTAG	6 cut(s)	699, 807, 1626, 2285, 2798, 2858
ZrmI	AGTACT	1 cut(s)	758
Zsp2I	ATGCAT	2 cut(s)	1281, 2080

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.