Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0009542

Physical Location & Seq

Genomic Coordinates Forward (+)

24865708 .. 24870886

Gene Structure 5179 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00020510.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2697 bp

ATGAGGAGAAGATCCTCGAGTTCTGGTCTGAAATCAATGCCCTTTGAGACCCAATTGGCTCGAACCAAGGACATGCCGGAGTATGTCTTCTACGACGGTCCAGCTTTTGCCACTGGCCTCCCACACTATGGCCACCTATTGGCTGGCACCATCAAGGACATTGTCACGCGCTACCAGTCCATGACTGGCCACCATGTCACGCGCCGCTTTGGCTGGGACTGCCATGGCTTGCCTGTTGAGACCGAGATCGATAAGAAGCTCAACATCAAGACCCGACAAGATGTTCTGAACCTGGGAATTGATGCCTACAATGAAGAATGTAGGAGCTTTGTTACTCGATATGTGGAGGAATGGGAGAAGGTGGTTACTCGCTCGGGGCGGTGGATCGATTTCAGGAATGATTATAAGACTATGGACTTGAAGTTCATGGAGAGTGTGTGGTGGGTTTTTGCTCAATTATATGAAAAGGGTCTTGTTTACAAAGACTTCAGGGTCATGCTTTACAGCACTGGTTGCAAGACACCGCTCTCCAATTTTGAGGCCGGTCAAAATCACAAGGATGTGTCTGATCCTGAAATAATGGTAGCTTTCCCAATTGTCGGTGATCCGGATAAGGCAGCGTTTGTGGCTTGGACAACTACTCCATGGACCCTTCCTAGCAATTTAGCCCTGTGGACTACACTAGTGGGAAAGAAGTATGAACCACTTTTTGATTACTTCATGGAGTTCTCAGATGTGGCATTTCGAGTTTTGTCCGACGATTATGTCAAAGGTGACAGTGGTACTGGAGTTGTTCACTGTGCTCCTGCATTTGAAAGAGTTGCTGACTTCAGCAACCTCTATGTGAAGGAGGCAGATAAGGCTATTATTGAAGCAGTGAGGCTAAAGGGAAGGCTTGTTAAGTCTGGAACCATAACTCATTCGTATCCCTTTTGCTGGAGAACAAACACTCCACTTATCTACAGAGCTGTTCCAAGCTGGTTTATCCGGGTGGAGCAGTTGAAAGAAAAGTTATTGGAAAACAACAAGCAGACATATTGGGTTCCTGATTTTGTGAAGGAAAAGCGATTCCACAATTGGCTAGAAAATGCAAGAGATTGGGCAGTTAGCAGAAGTAGATTTTGGGGTACTCCTCTTCCTGTGTGGGTTAGTGAGGATGGTGAGGAAGTACTGGTCATGGATTCCATTGAGAAGCTTGAAAAGCTTTCTGGTGTTAAGGTTTCTGATCTGCACCGCCACTACATTGATCATATCACTATTCCATCGGCGCGTGGCCCTGAATTTGGTTATCTTCGACGTGTAGACGATGTGTTTGATTGTTGGTTTGAGAGTGGATCCATGCCTTATGCTTATATCCACTATCCTTTCGAGAATGTGGAGCTTTTTGAGAAAAACTTTCCTGGGAACTTTGTGGCCGAAGGGCTTGATCAGACTCGCGGATGGTTCTACACTCTTATGGTGCTATCTACTGCATTATTTGGGAAACCTGCTTTTAAGAACCTCATTTGCAATGGACTTGTTCTTGCTGATGATGGGAAAAAGATGGGAAAAAGTTTTAAAAACTATCCTCCAGTGATGGAGGTTCTCGATCAATATGGGGCTGATGCAGTTCGAATGTATATTATTAACTCTCCTGTTGTTCGTGCAGAACCATTACGATTCAAGAAGGAAGGAGTATATGGAGTTGTCAAGGATAAGTCATCTAATGTGCTTGACCAGTGGATCAACTCAGCCACACAGTCTCTTGTGCATTTTGTCTGTCAAGAGATGGATGCTTACCGACTCTACACGGTGGTGCCATATCTTTTGAAGTTTCTTGACAACTTGACAAACATCTATAAAGTTCTGAATGATGCAGAGGAAAGCATTCATTACTGCAGTTTCCCTCAAGCACAAGGAAAGAGGGACGCTCGTATCGAGCAAAGTGTTGCCAGGATGAAGACAGTAATTGATCTTGCTCGTAATATCCGCGAACGTCACAACAAGCCTCTCAAAACACCCCTAAAAGAGATGGTCATTGTTCACCCTGATGCAGACTTTCTTGAAGACATTGATGCCAAGCTAAGAGAGTATGTGCTTGAAGAACTGAATGTGCAATCTCTAGTCCTGTGCAATGATACAGTGAAGTATACTTTGTCGCGCGCAGAGCCTGACTTCAGTGTGTTAGGTAAGCGGCTAGGAAAATCTTTGGGAGTTGTTTCCAAACAAGTCAAGGCCATGTCACAGGAGAGTATCTTAGAATTCGAGAAAACAGGAGAGGTTACTCTTTGTGGTCATTCTCTAAAGCTGAATGATATTAAGGTTGTTAGAGACTTCAAATGTCCTGATGGCACAACAGAGAAGGAGATTGACGCATCAGGAGATGGTGATGTTCTGGTGATCTTGGATTTGCATCAAGACGAATCATTGTTTGAGGCAGGTTTTGCTCGTGAGATTGTTAGCAGAGTTCAGAAGTTGAGGAAGAAATCTGGCCTTGGACTCACTGACATTGTGGAGGTGTACTTTGAGTCTTTGGATGAAGATAAATCAGTCTCGGACCGAGTTTTGCATTTGCAGGAACCATACATTAAGGATGCCATAGGTTCGCCAATTCTTCCTTGCGGTGTGAGGCCATCACATGCTGTTGTGGTTGGGGAGGAGAATTTCCGTGGAGTATCTGGAATGTCATTTAACATCAGTTTGGTTCGACCTATCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004822 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006428 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0017101 GO:0019538 GO:0019752 GO:0019899 GO:0032991 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0051020 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1

Protein Analysis

898

Amino Acids

102.81

Weight (kDa)

6.09

Isoelectric Point (pI)

39.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	14 - 550	1.5e-149	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g	PF09334	36 - 177	1.7e-06	tRNA synthetases class I (M)
DUF5915	PF19302	690 - 844	9.8e-25	Domain of unknown function (DUF5915)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009542)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G10320
fragaria_vesca	FvH4_4g11470
malus_domestica	MD04G1037200.v1.1 MD09G1065200.v1.1
prunus_persica	Prupe.1G186600_v2.0.a1 Prupe.1G186600_v2.0.a1
pyrus_communis	pycom04g03030 pycom111g05510
rosa_chinensis	RchiOBHm_Chr4g0408181
rosa_laevigata	RLG00000008666 RLG00000035346
rosa_multiflora	Rmu_ssc0000039.1_g000001
rosa_roxburghii	Rroxscaffold_1G00020510 Rroxscaffold_5G00352680
rosa_rugosa	Rorug04G0083900 Rorug04G0084000 Rorug05G0328300
rosa_samantha	Rh4AG148700 Rh4BG145500 Rh4CG155200 Rh4DG142000
rosa_wichuraiana	Rw4G012140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	405
AasI	GACNNNNNNGTC	2 cut(s)	491, 764
Acc36I	ACCTGC	2 cut(s)	1495, 2408
AccB1I	GGYRCC	2 cut(s)	146, 1795
AccB7I	CCANNNNNTGG	2 cut(s)	128, 139
AccBSI	CCGCTC	1 cut(s)	526
AccI	GTMKAC	2 cut(s)	1302, 2129
AccII	CGCG	7 cut(s)	169, 202, 1270, 1437, 1971, 2140, 2142
AccIII	TCCGGA	1 cut(s)	607
AciI	CCGC	8 cut(s)	205, 379, 524, 1234, 1437, 1969, 2173, 2601
AclWI	GGATC	7 cut(s)	6, 392, 563, 599, 1329, 1342, 1730
AcoI	YGGCCR	3 cut(s)	130, 187, 1413
AcsI	RAATTY	3 cut(s)	1280, 2240, 2641
AcuI	CTGAAG	3 cut(s)	472, 814, 2140
AfaI	GTAC	4 cut(s)	784, 1129, 1170, 2501
AfiI	CCNNNNNNNGG	5 cut(s)	128, 139, 599, 936, 1283
AflIII	ACRYGT	1 cut(s)	1297
AhlI	ACTAGT	1 cut(s)	682
AjiI	CACGTC	1 cut(s)	1298
AjnI	CCWGG	3 cut(s)	291, 1399, 1931
AjuI	GAANNNNNNNTTGG	2 cut(s)	1105, 1137
AleI	CACNNNNGTG	1 cut(s)	1793
AloI	GAACNNNNNNTCC	4 cut(s)	407, 439, 934, 966
Alw21I	GWGCWC	1 cut(s)	805
Alw26I	GTCTC	5 cut(s)	41, 233, 1746, 2304, 2536
AlwI	GGATC	7 cut(s)	6, 392, 563, 599, 1329, 1342, 1730
AlwNI	CAGNNNCTG	1 cut(s)	2150
Ama87I	CYCGRG	2 cut(s)	16, 373
Aor13HI	TCCGGA	1 cut(s)	607
AoxI	GGCC	9 cut(s)	115, 130, 187, 540, 1273, 1413, 2214, 2470, 2609
ApeKI	GCWGC	1 cut(s)	617
ApoI	RAATTY	3 cut(s)	1280, 2240, 2641
ArsI	GACNNNNNNTTYG	2 cut(s)	1295, 1327
Asp700I	GAANNNNTTC	1 cut(s)	1866
AspLEI	GCGC	5 cut(s)	171, 204, 1270, 2142, 2144
AspS9I	GGNCC	4 cut(s)	98, 648, 1274, 2536
AsuC2I	CCSGG	1 cut(s)	989
AsuHPI	GGTGA	6 cut(s)	614, 785, 1172, 2015, 2378, 2389
AsuII	TTCGAA	1 cut(s)	1612
AvaI	CYCGRG	2 cut(s)	16, 373
AvaII	GGWCC	3 cut(s)	98, 648, 2536
BaeI	ACNNNNGTAYC	4 cut(s)	774, 807, 1897, 1930
BalI	TGGCCA	2 cut(s)	132, 189
BamHI	GGATCC	1 cut(s)	1334
BanI	GGYRCC	2 cut(s)	146, 1795
BauI	CACGAG	1 cut(s)	2427
BbsI	GAAGAC	3 cut(s)	79, 1946, 2052
Bbv12I	GWGCWC	1 cut(s)	805
BbvI	GCAGC	1 cut(s)	629
BciT130I	CCWGG	3 cut(s)	293, 1401, 1933
BciVI	GTATCC	1 cut(s)	936
BclI	TGATCA	2 cut(s)	1246, 1426
BcnI	CCSGG	1 cut(s)	989
BcoDI	GTCTC	5 cut(s)	41, 233, 1746, 2304, 2536
BcuI	ACTAGT	1 cut(s)	682
BfaI	CTAG	6 cut(s)	657, 683, 1082, 2102, 2177, 2695
BfmI	CTRYAG	2 cut(s)	961, 1876
BfuAI	ACCTGC	2 cut(s)	1495, 2408
BfuI	GTATCC	1 cut(s)	936
BglI	GCCNNNNNGGC	1 cut(s)	210
BisI	GCNGC	3 cut(s)	205, 618, 2174
BlsI	GCNGC	3 cut(s)	206, 619, 2175
BmcAI	AGTACT	1 cut(s)	1170
Bme1390I	CCNGG	4 cut(s)	293, 989, 1401, 1933
Bme18I	GGWCC	3 cut(s)	98, 648, 2536
BmeT110I	CYCGRG	2 cut(s)	16, 373
BmgBI	CACGTC	1 cut(s)	1298
BmgT120I	GGNCC	4 cut(s)	98, 648, 1274, 2536
BmiI	GGNNCC	7 cut(s)	148, 650, 910, 1044, 1336, 1797, 2559
BmrFI	CCNGG	4 cut(s)	293, 989, 1401, 1933
BmsI	GCATC	9 cut(s)	292, 1594, 1762, 1843, 2020, 2044, 2363, 2401, 2563
BpiI	GAAGAC	3 cut(s)	79, 1946, 2052
BplI	GAGNNNNNCTC	2 cut(s)	1894, 1926
BpmI	CTGGAG	3 cut(s)	807, 958, 1554
Bpu14I	TTCGAA	1 cut(s)	1612
BpuEI	CTTGAG	1 cut(s)	1872
BpuMI	CCSGG	1 cut(s)	989
Bsa29I	ATCGAT	2 cut(s)	249, 387
BsaBI	GATNNNNATC	1 cut(s)	2391
BsaI	GGTCTC	2 cut(s)	41, 233
BsaJI	CCNNGG	7 cut(s)	66, 223, 292, 644, 1400, 2473, 2647
BsaWI	WCCGGW	1 cut(s)	607
BsaXI	ACNNNNNCTCC	6 cut(s)	422, 452, 625, 655, 934, 964
Bsc4I	CCNNNNNNNGG	5 cut(s)	128, 139, 599, 936, 1283
Bse118I	RCCGGY	1 cut(s)	542
Bse1I	ACTGG	8 cut(s)	118, 175, 190, 514, 790, 1176, 1571, 1717
Bse3DI	GCAATG	2 cut(s)	1516, 2119
Bse8I	GATNNNNATC	1 cut(s)	2391
BseAI	TCCGGA	1 cut(s)	607
BseBI	CCWGG	3 cut(s)	293, 1401, 1933
BseCI	ATCGAT	2 cut(s)	249, 387
BseDI	CCNNGG	7 cut(s)	66, 223, 292, 644, 1400, 2473, 2647
BseGI	GGATG	7 cut(s)	565, 1162, 1445, 1777, 1941, 2521, 2578
BseJI	GATNNNNATC	1 cut(s)	2391
BseLI	CCNNNNNNNGG	5 cut(s)	128, 139, 599, 936, 1283
BseMI	GCAATG	2 cut(s)	1516, 2119
BseMII	CTCAG	2 cut(s)	744, 1743
BseNI	ACTGG	8 cut(s)	118, 175, 190, 514, 790, 1176, 1571, 1717
BsePI	GCGCGC	1 cut(s)	2140
BseRI	GAGGAG	3 cut(s)	19, 1122, 2651
BseXI	GCAGC	1 cut(s)	629
BseYI	CCCAGC	1 cut(s)	213
BsgI	GTGCAG	2 cut(s)	1214, 1665
Bsh1236I	CGCG	7 cut(s)	169, 202, 1270, 1437, 1971, 2140, 2142
BshFI	GGCC	9 cut(s)	117, 132, 189, 542, 1275, 1415, 2216, 2472, 2611
BshNI	GGYRCC	2 cut(s)	146, 1795
BshVI	ATCGAT	2 cut(s)	249, 387
BsiHKAI	GWGCWC	1 cut(s)	805
BsiHKCI	CYCGRG	2 cut(s)	16, 373
BsiSI	CCGG	4 cut(s)	77, 543, 608, 988
BslFI	GGGAC	2 cut(s)	230, 1919
BslI	CCNNNNNNNGG	5 cut(s)	128, 139, 599, 936, 1283
BsmAI	GTCTC	5 cut(s)	41, 233, 1746, 2304, 2536
BsmFI	GGGAC	2 cut(s)	230, 1919
BsmI	GAATGC	1 cut(s)	1866
BsnI	GGCC	9 cut(s)	117, 132, 189, 542, 1275, 1415, 2216, 2472, 2611
Bso31I	GGTCTC	2 cut(s)	41, 233
BsoBI	CYCGRG	2 cut(s)	16, 373
Bsp119I	TTCGAA	1 cut(s)	1612
Bsp1286I	GDGCHC	1 cut(s)	805
Bsp13I	TCCGGA	1 cut(s)	607
Bsp19I	CCATGG	2 cut(s)	223, 644
BspACI	CCGC	8 cut(s)	205, 379, 524, 1234, 1437, 1969, 2173, 2601
BspANI	GGCC	9 cut(s)	117, 132, 189, 542, 1275, 1415, 2216, 2472, 2611
BspCNI	CTCAG	2 cut(s)	743, 1742
BspDI	ATCGAT	2 cut(s)	249, 387
BspEI	TCCGGA	1 cut(s)	607
BspFNI	CGCG	7 cut(s)	169, 202, 1270, 1437, 1971, 2140, 2142
BspLI	GGNNCC	7 cut(s)	148, 650, 910, 1044, 1336, 1797, 2559
BspMAI	CTGCAG	1 cut(s)	1880
BspMI	ACCTGC	2 cut(s)	1495, 2408
BspPI	GGATC	7 cut(s)	6, 392, 563, 599, 1329, 1342, 1730
BspT104I	TTCGAA	1 cut(s)	1612
BspT107I	GGYRCC	2 cut(s)	146, 1795
BspTNI	GGTCTC	2 cut(s)	41, 233
BsrBI	CCGCTC	1 cut(s)	526
BsrDI	GCAATG	2 cut(s)	1516, 2119
BsrFI	RCCGGY	1 cut(s)	542
BsrI	ACTGG	8 cut(s)	118, 175, 190, 514, 790, 1176, 1571, 1717
BssAI	RCCGGY	1 cut(s)	542
BssECI	CCNNGG	7 cut(s)	66, 223, 292, 644, 1400, 2473, 2647
BssHII	GCGCGC	1 cut(s)	2140
BssNAI	GTATAC	1 cut(s)	2130
BssSI	CACGAG	1 cut(s)	2427
BssT1I	CCWWGG	4 cut(s)	66, 223, 644, 2473
Bst1107I	GTATAC	1 cut(s)	2130
Bst2BI	CACGAG	1 cut(s)	2427
Bst2UI	CCWGG	3 cut(s)	293, 1401, 1933
Bst4CI	ACNGT	7 cut(s)	98, 779, 800, 1740, 1792, 1945, 2122
Bst6I	CTCTTC	1 cut(s)	1140
BstAPI	GCANNNNNTGC	2 cut(s)	513, 2423
BstBI	TTCGAA	1 cut(s)	1612
BstC8I	GCNNGC	3 cut(s)	145, 230, 2142
BstDEI	CTNAG	4 cut(s)	730, 1729, 2063, 2236
BstDSI	CCRYGG	3 cut(s)	223, 644, 2647
BstF5I	GGATG	7 cut(s)	565, 1162, 1445, 1777, 1941, 2521, 2578
BstFNI	CGCG	7 cut(s)	169, 202, 1270, 1437, 1971, 2140, 2142
BstHHI	GCGC	5 cut(s)	171, 204, 1270, 2142, 2144
BstMAI	GTCTC	5 cut(s)	41, 233, 1746, 2304, 2536
BstMWI	GCNNNNNNNGC	8 cut(s)	210, 219, 513, 626, 860, 1201, 2146, 2423
BstNI	CCWGG	3 cut(s)	293, 1401, 1933
BstNSI	RCATGY	2 cut(s)	76, 2621
BstSCI	CCNGG	4 cut(s)	291, 987, 1399, 1931
BstSFI	CTRYAG	2 cut(s)	961, 1876
BstUI	CGCG	7 cut(s)	169, 202, 1270, 1437, 1971, 2140, 2142
BstV1I	GCAGC	1 cut(s)	629
BstV2I	GAAGAC	3 cut(s)	79, 1946, 2052
BstX2I	RGATCY	2 cut(s)	11, 1334
BstYI	RGATCY	2 cut(s)	11, 1334
BstZ17I	GTATAC	1 cut(s)	2130
Bsu15I	ATCGAT	2 cut(s)	249, 387
BsuI	GTATCC	1 cut(s)	936
BsuRI	GGCC	9 cut(s)	117, 132, 189, 542, 1275, 1415, 2216, 2472, 2611
BsuTUI	ATCGAT	2 cut(s)	249, 387
BtgI	CCRYGG	3 cut(s)	223, 644, 2647
BtrI	CACGTC	1 cut(s)	1298
BtsCI	GGATG	7 cut(s)	565, 1162, 1445, 1777, 1941, 2521, 2578
BtsI	GCAGTG	1 cut(s)	882
BveI	ACCTGC	2 cut(s)	1495, 2408
Cac8I	GCNNGC	3 cut(s)	145, 230, 2142
CaiI	CAGNNNCTG	1 cut(s)	2150
CfoI	GCGC	5 cut(s)	171, 204, 1270, 2142, 2144
Cfr10I	RCCGGY	1 cut(s)	542
Cfr13I	GGNCC	4 cut(s)	98, 648, 1274, 2536
ClaI	ATCGAT	2 cut(s)	249, 387
CpoI	CGGWCCG	1 cut(s)	2536
CseI	GACGC	2 cut(s)	1916, 2360
Csp6I	GTAC	4 cut(s)	783, 1128, 1169, 2500
CspI	CGGWCCG	1 cut(s)	2536
CviQI	GTAC	4 cut(s)	783, 1128, 1169, 2500
DdeI	CTNAG	4 cut(s)	730, 1729, 2063, 2236
DraI	TTTAAA	1 cut(s)	1558
DrdI	GACNNNNNNGTC	2 cut(s)	491, 764
DseDI	GACNNNNNNGTC	2 cut(s)	491, 764
EaeI	YGGCCR	3 cut(s)	130, 187, 1413
Eam1104I	CTCTTC	1 cut(s)	1140
EarI	CTCTTC	1 cut(s)	1140
Eco130I	CCWWGG	4 cut(s)	66, 223, 644, 2473
Eco31I	GGTCTC	2 cut(s)	41, 233
Eco47I	GGWCC	3 cut(s)	98, 648, 2536
Eco57I	CTGAAG	3 cut(s)	472, 814, 2140
Eco88I	CYCGRG	2 cut(s)	16, 373
EcoRI	GAATTC	1 cut(s)	2240
EcoRII	CCWGG	3 cut(s)	291, 1399, 1931
EcoT14I	CCWWGG	4 cut(s)	66, 223, 644, 2473
ErhI	CCWWGG	4 cut(s)	66, 223, 644, 2473
FaqI	GGGAC	2 cut(s)	230, 1919
FbaI	TGATCA	2 cut(s)	1246, 1426
FblI	GTMKAC	2 cut(s)	1302, 2129
Fnu4HI	GCNGC	3 cut(s)	205, 618, 2174
FokI	GGATG	7 cut(s)	572, 1169, 1452, 1784, 1948, 2528, 2585
Fsp4HI	GCNGC	3 cut(s)	205, 618, 2174
FspBI	CTAG	6 cut(s)	657, 683, 1082, 2102, 2177, 2695
GlaI	GCGC	5 cut(s)	170, 203, 1269, 2141, 2143
GluI	GCNGC	3 cut(s)	205, 618, 2174
GsaI	CCCAGC	1 cut(s)	217
GsuI	CTGGAG	3 cut(s)	807, 958, 1554
HaeIII	GGCC	9 cut(s)	117, 132, 189, 542, 1275, 1415, 2216, 2472, 2611
HapII	CCGG	4 cut(s)	77, 543, 608, 988
HgaI	GACGC	2 cut(s)	1916, 2360
HhaI	GCGC	5 cut(s)	171, 204, 1270, 2142, 2144
Hin6I	GCGC	5 cut(s)	169, 202, 1268, 2140, 2142
HinP1I	GCGC	5 cut(s)	169, 202, 1268, 2140, 2142
HindIII	AAGCTT	2 cut(s)	1193, 1202
HinfI	GANTC	8 cut(s)	1068, 1181, 1432, 1659, 1782, 2402, 2478, 2507
HpaII	CCGG	4 cut(s)	77, 543, 608, 988
HphI	GGTGA	6 cut(s)	614, 785, 1172, 2015, 2378, 2389
Hpy166II	GTNNAC	7 cut(s)	478, 675, 796, 1303, 2023, 2130, 2500
Hpy8I	GTNNAC	7 cut(s)	478, 675, 796, 1303, 2023, 2130, 2500
Hpy99I	CGWCG	3 cut(s)	98, 761, 1299
HpyAV	CCTTC	9 cut(s)	352, 662, 841, 885, 1051, 1412, 1660, 1664, 2335
HpyCH4III	ACNGT	7 cut(s)	98, 779, 800, 1740, 1792, 1945, 2122
HpyCH4IV	ACGT	2 cut(s)	1297, 1975
HpyF10VI	GCNNNNNNNGC	8 cut(s)	210, 219, 513, 626, 860, 1201, 2146, 2423
HpyF3I	CTNAG	4 cut(s)	730, 1729, 2063, 2236
HpySE526I	ACGT	2 cut(s)	1297, 1975
HspAI	GCGC	5 cut(s)	169, 202, 1268, 2140, 2142
Kpn2I	TCCGGA	1 cut(s)	607
Ksp22I	TGATCA	2 cut(s)	1246, 1426
LmnI	GCTCC	4 cut(s)	324, 808, 994, 1378
Lsp1109I	GCAGC	1 cut(s)	629
LweI	GCATC	9 cut(s)	292, 1594, 1762, 1843, 2020, 2044, 2363, 2401, 2563
MaeI	CTAG	6 cut(s)	657, 683, 1082, 2102, 2177, 2695
MaeII	ACGT	2 cut(s)	1297, 1975
MaeIII	GTNAC	8 cut(s)	163, 196, 331, 364, 773, 1976, 2220, 2260
MbiI	CCGCTC	1 cut(s)	526
MfeI	CAATTG	3 cut(s)	53, 594, 1075
MflI	RGATCY	2 cut(s)	11, 1334
MhlI	GDGCHC	1 cut(s)	805
MlsI	TGGCCA	2 cut(s)	132, 189
MluNI	TGGCCA	2 cut(s)	132, 189
MlyI	GAGTC	4 cut(s)	1426, 1776, 2472, 2516
MmeI	TCCRAC	1 cut(s)	780
Mox20I	TGGCCA	2 cut(s)	132, 189
MroI	TCCGGA	1 cut(s)	607
MroXI	GAANNNNTTC	1 cut(s)	1866
MscI	TGGCCA	2 cut(s)	132, 189
MseI	TTAA	8 cut(s)	900, 1215, 1494, 1557, 1626, 2298, 2568, 2670
MslI	CAYNNNNRTG	4 cut(s)	558, 1454, 1793, 2028
Msp20I	TGGCCA	2 cut(s)	132, 189
MspI	CCGG	4 cut(s)	77, 543, 608, 988
MspR9I	CCNGG	4 cut(s)	293, 989, 1401, 1933
MunI	CAATTG	3 cut(s)	53, 594, 1075
Mva1269I	GAATGC	1 cut(s)	1866
MvaI	CCWGG	3 cut(s)	293, 1401, 1933
MvnI	CGCG	7 cut(s)	169, 202, 1270, 1437, 1971, 2140, 2142
MwoI	GCNNNNNNNGC	8 cut(s)	210, 219, 513, 626, 860, 1201, 2146, 2423
NciI	CCSGG	1 cut(s)	989
NcoI	CCATGG	2 cut(s)	223, 644
NlaIV	GGNNCC	7 cut(s)	148, 650, 910, 1044, 1336, 1797, 2559
NmuCI	GTSAC	5 cut(s)	163, 196, 773, 1976, 2220
NspI	RCATGY	2 cut(s)	76, 2621
NspV	TTCGAA	1 cut(s)	1612
OliI	CACNNNNGTG	1 cut(s)	1793
PaeR7I	CTCGAG	1 cut(s)	16
PauI	GCGCGC	1 cut(s)	2140
PcsI	WCGNNNNNNNCGW	1 cut(s)	1914
PctI	GAATGC	1 cut(s)	1866
PdmI	GAANNNNTTC	1 cut(s)	1866
PfeI	GAWTC	4 cut(s)	1068, 1181, 1659, 2402
PflFI	GACNNNGTC	1 cut(s)	161
PflMI	CCANNNNNTGG	2 cut(s)	128, 139
PkrI	GCNGC	3 cut(s)	206, 619, 2175
PleI	GAGTC	4 cut(s)	1426, 1776, 2472, 2515
PpsI	GAGTC	4 cut(s)	1426, 1776, 2472, 2515
PsiI	TTATAA	1 cut(s)	405
Psp6I	CCWGG	3 cut(s)	291, 1399, 1931
PspFI	CCCAGC	1 cut(s)	213
PspGI	CCWGG	3 cut(s)	291, 1399, 1931
PspN4I	GGNNCC	7 cut(s)	148, 650, 910, 1044, 1336, 1797, 2559
PspPI	GGNCC	4 cut(s)	98, 648, 1274, 2536
PspXI	VCTCGAGB	1 cut(s)	16
PstI	CTGCAG	1 cut(s)	1880
PstNI	CAGNNNCTG	1 cut(s)	2150
PsuI	RGATCY	2 cut(s)	11, 1334
PsyI	GACNNNGTC	1 cut(s)	161
PteI	GCGCGC	1 cut(s)	2140
RsaI	GTAC	4 cut(s)	784, 1129, 1170, 2501
RsaNI	GTAC	4 cut(s)	783, 1128, 1169, 2500
RseI	CAYNNNNRTG	4 cut(s)	558, 1454, 1793, 2028
Rsr2I	CGGWCCG	1 cut(s)	2536
RsrII	CGGWCCG	1 cut(s)	2536
SaqAI	TTAA	8 cut(s)	900, 1215, 1494, 1557, 1626, 2298, 2568, 2670
SatI	GCNGC	3 cut(s)	205, 618, 2174
Sau96I	GGNCC	4 cut(s)	98, 648, 1274, 2536
ScaI	AGTACT	1 cut(s)	1170
SchI	GAGTC	4 cut(s)	1426, 1776, 2472, 2516
ScrFI	CCNGG	4 cut(s)	293, 989, 1401, 1933
SduI	GDGCHC	1 cut(s)	805
SfaNI	GCATC	9 cut(s)	292, 1594, 1762, 1843, 2020, 2044, 2363, 2401, 2563
SfcI	CTRYAG	2 cut(s)	961, 1876
Sfr274I	CTCGAG	1 cut(s)	16
SfuI	TTCGAA	1 cut(s)	1612
SinI	GGWCC	3 cut(s)	98, 648, 2536
SlaI	CTCGAG	1 cut(s)	16
SmiMI	CAYNNNNRTG	4 cut(s)	558, 1454, 1793, 2028
SmlI	CTYRAG	2 cut(s)	16, 1887
SmoI	CTYRAG	2 cut(s)	16, 1887
SpeI	ACTAGT	1 cut(s)	682
SsiI	CCGC	8 cut(s)	205, 379, 524, 1234, 1437, 1969, 2173, 2601
SspMI	CTAG	6 cut(s)	657, 683, 1082, 2102, 2177, 2695
StyD4I	CCNGG	4 cut(s)	291, 987, 1399, 1931
StyI	CCWWGG	4 cut(s)	66, 223, 644, 2473
TaaI	ACNGT	7 cut(s)	98, 779, 800, 1740, 1792, 1945, 2122
TaiI	ACGT	2 cut(s)	1300, 1978
TaqII	GACCGA	2 cut(s)	257, 2553
TatI	WGTACW	2 cut(s)	1168, 2499
TauI	GCSGC	2 cut(s)	207, 2176
TfiI	GAWTC	4 cut(s)	1068, 1181, 1659, 2402
Tru1I	TTAA	8 cut(s)	900, 1215, 1494, 1557, 1626, 2298, 2568, 2670
Tru9I	TTAA	8 cut(s)	900, 1215, 1494, 1557, 1626, 2298, 2568, 2670
TseFI	GTSAC	5 cut(s)	163, 196, 773, 1976, 2220
TseI	GCWGC	1 cut(s)	617
Tsp45I	GTSAC	5 cut(s)	163, 196, 773, 1976, 2220
TspDTI	ATGAA	8 cut(s)	327, 415, 477, 709, 714, 1859, 1952, 2532
TspGWI	ACGGA	1 cut(s)	2636
Tth111I	GACNNNGTC	1 cut(s)	161
Van91I	CCANNNNNTGG	2 cut(s)	128, 139
VpaK11BI	GGWCC	3 cut(s)	98, 648, 2536
XapI	RAATTY	3 cut(s)	1280, 2240, 2641
XceI	RCATGY	2 cut(s)	76, 2621
XcmI	CCANNNNNNNNNTGG	2 cut(s)	140, 182
XhoI	CTCGAG	1 cut(s)	16
XmiI	GTMKAC	2 cut(s)	1302, 2129
XmnI	GAANNNNTTC	1 cut(s)	1866
XspI	CTAG	6 cut(s)	657, 683, 1082, 2102, 2177, 2695
ZrmI	AGTACT	1 cut(s)	1170

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.