Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0009542

Physical Location & Seq

Genomic Coordinates Forward (+)

4447803 .. 4452408

Gene Structure 4606 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1065200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3318 bp

ATGGAGGAAGTGTGCGAGGGCAAGGACTTCTCCTTCCCCAAACAAGAAGAAACCATCCTCAAACTCTGGTCCGAAATCAAAGCCTTCGAAACCCAATTGGCGCGCACGGAGGGCCAGCCGGAATACGTCTTCTATGACGGCCCTCCGTTTGCCACCGGCCTCCCGCACTACGGCCACATCTTGGCCGGAACCATCAAAGACATCATCACCCGATTCTTCTCCATGACCGGCCACCACGTCATCCGCCGATTCGGGTGGGACTGCCACGGCCTGCCTGTGGAGAACGAGATCGATCGGACTCACGGTATCAAGCGCCGGGACGACGTGCTGAATATGGGGATCGATAAGTACAACGAGGCCTGTAGAAGCATTGTGACGCGGTACGTCGGCGAGTGGGAGAAGGTTATCACTCGGACGGGGCGGTGGATTGATTTCCAGAACGATTACAAGACGATGGACTTGAAGTTTATGGAATCTGTGTGGTGGGTTTTTGCGCAATTGTTCGAAAAGGGGCTGGTTTATAAAGGATTCAAGGTCATGCCATACAGCACTGGTTGCAAGACACCACTCTCAAATTTTGAGGCTGGTCAAGATTATAGGGATGTGCCTGATCCCGAAATTATGGTGACTTTTCCAATTGTGGGAGATCTGGAAAATGCAAGTTTTGTGGCTTGGACAACTACACCATGGACCCTCCCTAGTAACTTAGCCCTCTGTGTCAATGCGAACTTTACTTATGTGAAGGTTCGCAACAACTACTCGGGGAAAGTTTATGTTGTTGCTGAATCTCGGTTGTCAGCTCTTCCTAGCGAGAAACCTAAAGAGAAAAGTACTGTAGTAGAAAATGGGTCCAAGTCTAAGACCAAGGGAGGAGCTGGGAAAAAGGAAACTGTCGCCAGTTCATCATATCAAGTATTGCAGAAATTCTCGGGGGCTTCATTAGTGGGAAATAAGTATGTGCCACTGTTTGAATACTTTAAGGAGTTCTCTGATGTGGCATTTAGAGTTGTTGTGGACAACTATGTTACTGATGATAGTGGGACTGGTATTGTTCATTGTGCTCCTGCCTTTGGCGAGGATGATTATCGTGTTTGCCTCGAAAACCAAGTAATCAATAAGGGAGACAACTTGATTGTAGCGGTTGATGATGATGGCTGCTTTACAGAAAGAATCACTGATTTCAGCGGGTTCTATGTCAAGGATGCCGATAAAGATATTATTGAAGCAGTGAAGGCAAAAGGAAGGCTAGTGAAGTCCGGAACTTTCACTCATTCTTATCCCTTTTGCTGGAGATCTAAAACTCCACTTATCTACAGAGCTGTCCCAAGTTGGTTTATCCGGGTGGAGCAGTTGAAAGAAAAATTGCTAGAAAATAACACACAGACTTACTGGGTTCCTGACTTTGTCAAGGAAAAGCGCTTCCACAATTGGCTGGAAAATGCAAGAGATTGGGCCGTTAGTCGAAGTAGGTTTTGGGGAACTCCTCTCCCTGTGTGGGTTAGTGAGGATGGCGAGGAGATACTAGTCATCGATTCTATTGATAAGCTTGAAAAGCTTTCCGGTGTTAAGGTTTTTGATCTGCACCGGCACAATATCGATCATATCACTATTCCATCTGCTCGTGGTCCGCAACATGGTGTTCTTCGACGTATAGATGATGTGTTTGATTGCTGGTTTGAGAGTGGATCCATGCCTTATGCTTATATCCACTATCCTTTCGAGAATGTTGAACTTTTTGAGAAAAATTTTCCTGGGCATTTCGTCGCGGAAGGGCTAGATCAGACTCGTGGGTGGTTCTACACTCTTATGGTGTTATCTACTGCGTTATTTGGGAAACCTGCTTTTAGAAACCTGATTTGCAATGGACTTGTCCTTGCTGAGGATGGAAAAAAAATGAGTAAAAGTTTGAAGAACTATCCTTCACCAATGGAGGTTATTGATGACTATGGTGCTGATGCACTACGATTATACCTCATCAACTCTCCTGTTGTGCGTGCGGAGACATTACGTTTCAAGAAGGAAGGAGTTTTTGGTGTGGTCAAGGATGTATTTCTTCCTTGGTACAATGCATACAGGTTCCTTGTTCAGAATGCAAAGAGACTTGAAGATGAAGGGTTTGGCACTTTCCTCCCTATTGATCTAACCACTGTACAAAAATCATCTAATGTGCTTGACCAGTGGATCAACTCAGCCACACAGAGTCTTGTGCATTTTGTCCAACAAGAGATGAATGGTTATCGACTTTACACGGTGGTTCCATATCTTTTGAAGTTTCTTGACAACTTGACAAATATTTATGTGCGGTTCAATCGCAAGAGGCTAAAGGGTCGTACTGGTGAAGAAGATTGTAGGATGGCACTCTCAACTCTTTATAATGTCCTCTTGGTGTCCTGTAAAGTGATGGCACCATTAACGCCATTCTTCACTGAGGTGCTTTATCAAAATTTGCGGAAAGTTTTGAATGAGTCTGAGGAAAGCATTCACTTCTGTAGTTTTCCTAAAGCAGAAGGAAAGAGGGATGAACGCATGGAGCAGAGTGTTAGAAGAATGATGACAGTGATAGATCTTGGCCGTAACATTCGTGAGCGTCACAATAAGCCTCTCAAGACACCCCTTAAGGAGATGGTTATAGTTCATCCTGATGCAGACTTTCTTGATGATATTGCTGGAAAGCTAAGAGATTATGTGTTGGAGGAACTTAATGTACGATCTCTTGTCACATGTAATGATACATTGGAGTATGCTTCGTTACGTGCAGAGCCTGATTTTAGTGTACTGGGTAAGCGGCTTGGAAAATCGATGGGAGTTGTTGCCAAAGAAGTCAAGGCCATGTCCCAGGAAAGCATTTTAGCATTTGAGAAAGCTGGAGAAGTTACTGTTTCTGGTCATTGTTTAAAGCTTGCTGATATTAAGGTGGTTAGGGATTTCAAGCGTCCTAATGGAATGACAGAAAAGGAGATTGATGCAGCGGGAGATGGTGATGTTTTGGTGGTTTTGGATTTACGTCCAGATGAGTCCTTGTTTGAGGCTGGTGTTGCTCGCGAAATTGTTAGGAGAGTTCAACAGTTACGGAAGACAGCTGCCCTTAAACCTACTGACGTGGTAGAGGCCTACTTTGAATCTTTAGATCAAGATAAATCAGTCTCACAACGGGTTTTGCAATCCCAGGAAGAATACATTACAGATGCCATTGGTTTTCCATTGCGTCCTTCTCATGTGATGCCATCAGATGCTGTCCTTGTTGCGGAGGAGAGTTCCCATGGGGTATCTGGTATGTCATTTGTTATCAGATTGGTTCGCCCGACCAGTTCCAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004822 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006428 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0017101 GO:0019538 GO:0019752 GO:0019899 GO:0032991 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0051020 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1

Protein Analysis

1106

Amino Acids

126.01

Weight (kDa)

6.14

Isoelectric Point (pI)

39.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	19 - 669	3.5e-156	tRNA synthetases class I (I, L, M and V)
Anticodon_1	PF08264	725 - 874	3.9e-27	Anticodon-binding domain of tRNA ligase
DUF5915	PF19302	894 - 1061	1.8e-24	Domain of unknown function (DUF5915)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009542)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G10320
fragaria_vesca	FvH4_4g11470
malus_domestica	MD04G1037200.v1.1 MD09G1065200.v1.1
prunus_persica	Prupe.1G186600_v2.0.a1 Prupe.1G186600_v2.0.a1
pyrus_communis	pycom04g03030 pycom111g05510
rosa_chinensis	RchiOBHm_Chr4g0408181
rosa_laevigata	RLG00000008666 RLG00000035346
rosa_multiflora	Rmu_ssc0000039.1_g000001
rosa_roxburghii	Rroxscaffold_1G00020510 Rroxscaffold_5G00352680
rosa_rugosa	Rorug04G0083900 Rorug04G0084000 Rorug05G0328300
rosa_samantha	Rh4AG148700 Rh4BG145500 Rh4CG155200 Rh4DG142000
rosa_wichuraiana	Rw4G012140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	522, 2373
Acc16I	TGCGCA	1 cut(s)	495
Acc36I	ACCTGC	1 cut(s)	1846
AccB1I	GGYRCC	1 cut(s)	2404
AccB7I	CCANNNNNTGG	1 cut(s)	181
AccII	CGCG	4 cut(s)	103, 379, 1766, 3042
AccIII	TCCGGA	1 cut(s)	1256
AclWI	GGATC	5 cut(s)	347, 605, 1680, 1693, 2189
AcoI	YGGCCR	4 cut(s)	172, 183, 229, 2569
AcsI	RAATTY	4 cut(s)	574, 923, 1744, 2443
AfaI	GTAC	8 cut(s)	350, 383, 832, 2063, 2151, 2332, 2706, 2775
AfeI	AGCGCT	1 cut(s)	1418
AflII	CTTAAG	1 cut(s)	2615
AflIII	ACRYGT	1 cut(s)	2720
AhlI	ACTAGT	1 cut(s)	1522
AjiI	CACGTC	3 cut(s)	238, 325, 3100
AjnI	CCWGG	3 cut(s)	1750, 2835, 3165
AjuI	GAANNNNNNNTTGG	4 cut(s)	1456, 1488, 2013, 2045
AleI	CACNNNNGTG	1 cut(s)	2429
AluBI	AGCT	9 cut(s)	800, 875, 1319, 1546, 1555, 2674, 2864, 2899, 3080
AluI	AGCT	9 cut(s)	800, 875, 1319, 1546, 1555, 2674, 2864, 2899, 3080
Alw21I	GWGCWC	1 cut(s)	1063
Alw26I	GTCTC	4 cut(s)	1116, 1994, 2092, 3148
AlwI	GGATC	5 cut(s)	347, 605, 1680, 1693, 2189
AlwNI	CAGNNNCTG	2 cut(s)	2762, 3233
Ama87I	CYCGRG	2 cut(s)	760, 928
Aor13HI	TCCGGA	1 cut(s)	1256
Aor51HI	AGCGCT	1 cut(s)	1418
ApeKI	GCWGC	3 cut(s)	1155, 2966, 3080
ApoI	RAATTY	4 cut(s)	574, 923, 1744, 2443
Asp700I	GAANNNNTTC	1 cut(s)	2478
AspLEI	GCGC	5 cut(s)	103, 105, 315, 496, 1419
AspS9I	GGNCC	7 cut(s)	69, 112, 140, 690, 849, 1452, 1625
AsuC2I	CCSGG	2 cut(s)	317, 1340
AsuHPI	GGTGA	5 cut(s)	199, 637, 1914, 2348, 2990
AsuII	TTCGAA	2 cut(s)	87, 504
AvaI	CYCGRG	2 cut(s)	760, 928
AvaII	GGWCC	4 cut(s)	69, 690, 849, 1625
BamHI	GGATCC	1 cut(s)	1685
BanI	GGYRCC	1 cut(s)	2404
BauI	CACGAG	2 cut(s)	1620, 1785
BbsI	GAAGAC	2 cut(s)	121, 3080
Bbv12I	GWGCWC	1 cut(s)	1063
BbvCI	CCTCAGC	1 cut(s)	1878
BbvI	GCAGC	3 cut(s)	1142, 2978, 3067
BceAI	ACGGC	5 cut(s)	154, 187, 283, 1439, 2556
BciT130I	CCWGG	3 cut(s)	1752, 2837, 3167
BcnI	CCSGG	2 cut(s)	317, 1340
BcoDI	GTCTC	4 cut(s)	1116, 1994, 2092, 3148
BcuI	ACTAGT	1 cut(s)	1522
BfaI	CTAG	6 cut(s)	699, 807, 1247, 1367, 1523, 1775
BfmI	CTRYAG	4 cut(s)	361, 834, 1312, 2488
BfoI	RGCGCY	2 cut(s)	316, 1420
BfrI	CTTAAG	1 cut(s)	2615
BfuAI	ACCTGC	1 cut(s)	1846
BglII	AGATCT	3 cut(s)	646, 1292, 2563
BisI	GCNGC	4 cut(s)	1156, 2786, 2967, 3081
BlsI	GCNGC	4 cut(s)	1157, 2787, 2968, 3082
BmcAI	AGTACT	1 cut(s)	832
Bme1390I	CCNGG	5 cut(s)	317, 1340, 1752, 2837, 3167
Bme18I	GGWCC	4 cut(s)	69, 690, 849, 1625
BmeT110I	CYCGRG	2 cut(s)	760, 928
BmgBI	CACGTC	3 cut(s)	238, 325, 3100
BmgT120I	GGNCC	7 cut(s)	69, 112, 140, 690, 849, 1452, 1625
BmiI	GGNNCC	8 cut(s)	190, 692, 850, 1395, 1687, 2078, 2256, 2406
BmrFI	CCNGG	5 cut(s)	317, 1340, 1752, 2837, 3167
BmrI	ACTGGG	2 cut(s)	1399, 2786
BmsI	GCATC	7 cut(s)	1192, 1945, 2632, 2953, 3175, 3210, 3220
BmuI	ACTGGG	2 cut(s)	1399, 2786
BpiI	GAAGAC	2 cut(s)	121, 3080
BpmI	CTGGAG	2 cut(s)	1309, 2886
Bpu10I	CCTNAGC	1 cut(s)	1878
Bpu14I	TTCGAA	2 cut(s)	87, 504
BpuEI	CTTGAG	1 cut(s)	2588
BpuMI	CCSGG	2 cut(s)	317, 1340
Bsa29I	ATCGAT	5 cut(s)	291, 342, 1530, 1596, 2798
BsaAI	YACGTR	1 cut(s)	2753
BsaBI	GATNNNNATC	1 cut(s)	1083
BsaJI	CCNNGG	8 cut(s)	265, 686, 864, 1751, 2057, 2835, 3165, 3259
BsaWI	WCCGGW	2 cut(s)	1256, 1559
BsaXI	ACNNNNNCTCC	1 cut(s)	26
Bse118I	RCCGGY	3 cut(s)	155, 227, 1584
Bse1I	ACTGG	8 cut(s)	556, 897, 1048, 1394, 2176, 2338, 2781, 3306
Bse3DI	GCAATG	2 cut(s)	1867, 3200
Bse8I	GATNNNNATC	1 cut(s)	1083
BseAI	TCCGGA	1 cut(s)	1256
BseBI	CCWGG	3 cut(s)	1752, 2837, 3167
BseCI	ATCGAT	5 cut(s)	291, 342, 1530, 1596, 2798
BseDI	CCNNGG	8 cut(s)	265, 686, 864, 1751, 2057, 2835, 3165, 3259
BseJI	GATNNNNATC	1 cut(s)	1083
BseMI	GCAATG	2 cut(s)	1867, 3200
BseMII	CTCAG	4 cut(s)	1869, 2202, 2418, 2460
BseNI	ACTGG	8 cut(s)	556, 897, 1048, 1394, 2176, 2338, 2781, 3306
BsePI	GCGCGC	1 cut(s)	101
BseRI	GAGGAG	4 cut(s)	885, 1473, 1529, 3263
BseXI	GCAGC	3 cut(s)	1142, 2978, 3067
BseYI	CCCAGC	1 cut(s)	875
BsgI	GTGCAG	2 cut(s)	1565, 2775
Bsh1236I	CGCG	4 cut(s)	103, 379, 1766, 3042
Bsh1285I	CGRYCG	1 cut(s)	295
BshNI	GGYRCC	1 cut(s)	2404
BshVI	ATCGAT	5 cut(s)	291, 342, 1530, 1596, 2798
BsiEI	CGRYCG	1 cut(s)	295
BsiHKAI	GWGCWC	1 cut(s)	1063
BsiHKCI	CYCGRG	2 cut(s)	760, 928
BsiSI	CCGG	9 cut(s)	119, 156, 186, 228, 316, 1257, 1339, 1560, 1585
BslFI	GGGAC	5 cut(s)	272, 332, 1054, 1307, 2818
BsmAI	GTCTC	4 cut(s)	1116, 1994, 2092, 3148
BsmFI	GGGAC	5 cut(s)	272, 332, 1054, 1307, 2818
BsmI	GAATGC	2 cut(s)	2095, 2478
BsoBI	CYCGRG	2 cut(s)	760, 928
Bsp119I	TTCGAA	2 cut(s)	87, 504
Bsp1286I	GDGCHC	1 cut(s)	1063
Bsp13I	TCCGGA	1 cut(s)	1256
Bsp1407I	TGTACA	1 cut(s)	2149
Bsp19I	CCATGG	2 cut(s)	686, 3259
Bsp68I	TCGCGA	1 cut(s)	3042
BspCNI	CTCAG	4 cut(s)	1870, 2201, 2419, 2461
BspDI	ATCGAT	5 cut(s)	291, 342, 1530, 1596, 2798
BspEI	TCCGGA	1 cut(s)	1256
BspFNI	CGCG	4 cut(s)	103, 379, 1766, 3042
BspLI	GGNNCC	8 cut(s)	190, 692, 850, 1395, 1687, 2078, 2256, 2406
BspMI	ACCTGC	1 cut(s)	1846
BspPI	GGATC	5 cut(s)	347, 605, 1680, 1693, 2189
BspQI	GCTCTTC	1 cut(s)	807
BspT104I	TTCGAA	2 cut(s)	87, 504
BspT107I	GGYRCC	1 cut(s)	2404
BspTI	CTTAAG	1 cut(s)	2615
BsrDI	GCAATG	2 cut(s)	1867, 3200
BsrFI	RCCGGY	3 cut(s)	155, 227, 1584
BsrGI	TGTACA	1 cut(s)	2149
BsrI	ACTGG	8 cut(s)	556, 897, 1048, 1394, 2176, 2338, 2781, 3306
BssAI	RCCGGY	3 cut(s)	155, 227, 1584
BssECI	CCNNGG	8 cut(s)	265, 686, 864, 1751, 2057, 2835, 3165, 3259
BssHII	GCGCGC	1 cut(s)	101
BssSI	CACGAG	2 cut(s)	1620, 1785
BssT1I	CCWWGG	4 cut(s)	686, 864, 2057, 3259
Bst2BI	CACGAG	2 cut(s)	1620, 1785
Bst2UI	CCWGG	3 cut(s)	1752, 2837, 3167
Bst4CI	ACNGT	9 cut(s)	305, 835, 892, 966, 2149, 2251, 2557, 2878, 3066
Bst6I	CTCTTC	1 cut(s)	807
BstAFI	CTTAAG	1 cut(s)	2615
BstAPI	GCANNNNNTGC	1 cut(s)	555
BstAUI	TGTACA	1 cut(s)	2149
BstBAI	YACGTR	1 cut(s)	2753
BstBI	TTCGAA	2 cut(s)	87, 504
BstC8I	GCNNGC	6 cut(s)	103, 116, 272, 1995, 2901, 3040
BstDEI	CTNAG	7 cut(s)	706, 858, 1878, 2188, 2427, 2469, 2675
BstDSI	CCRYGG	3 cut(s)	265, 686, 3259
BstENI	CCTNNNNNAGG	1 cut(s)	1877
BstFNI	CGCG	4 cut(s)	103, 379, 1766, 3042
BstH2I	RGCGCY	2 cut(s)	316, 1420
BstHHI	GCGC	5 cut(s)	103, 105, 315, 496, 1419
BstMAI	GTCTC	4 cut(s)	1116, 1994, 2092, 3148
BstMCI	CGRYCG	1 cut(s)	295
BstMWI	GCNNNNNNNGC	5 cut(s)	111, 555, 1232, 1552, 3035
BstNI	CCWGG	3 cut(s)	1752, 2837, 3167
BstNSI	RCATGY	1 cut(s)	2724
BstSCI	CCNGG	5 cut(s)	315, 1338, 1750, 2835, 3165
BstSFI	CTRYAG	4 cut(s)	361, 834, 1312, 2488
BstUI	CGCG	4 cut(s)	103, 379, 1766, 3042
BstV1I	GCAGC	3 cut(s)	1142, 2978, 3067
BstV2I	GAAGAC	2 cut(s)	121, 3080
BstX2I	RGATCY	4 cut(s)	646, 1292, 1685, 2563
BstYI	RGATCY	4 cut(s)	646, 1292, 1685, 2563
Bsu15I	ATCGAT	5 cut(s)	291, 342, 1530, 1596, 2798
BsuTUI	ATCGAT	5 cut(s)	291, 342, 1530, 1596, 2798
BtgI	CCRYGG	3 cut(s)	265, 686, 3259
BtrI	CACGTC	3 cut(s)	238, 325, 3100
BtsI	GCAGTG	1 cut(s)	1233
BtsIMutI	CAGTG	8 cut(s)	549, 962, 1173, 1233, 2145, 2183, 2424, 2562
BtuMI	TCGCGA	1 cut(s)	3042
BveI	ACCTGC	1 cut(s)	1846
Cac8I	GCNNGC	6 cut(s)	103, 116, 272, 1995, 2901, 3040
CaiI	CAGNNNCTG	2 cut(s)	2762, 3233
CfoI	GCGC	5 cut(s)	103, 105, 315, 496, 1419
Cfr10I	RCCGGY	3 cut(s)	155, 227, 1584
Cfr13I	GGNCC	7 cut(s)	69, 112, 140, 690, 849, 1452, 1625
ClaI	ATCGAT	5 cut(s)	291, 342, 1530, 1596, 2798
CseI	GACGC	4 cut(s)	385, 2576, 2921, 3194
Csp6I	GTAC	8 cut(s)	349, 382, 831, 2062, 2150, 2331, 2705, 2774
CspCI	CAANNNNNGTGG	2 cut(s)	648, 683
CviQI	GTAC	8 cut(s)	349, 382, 831, 2062, 2150, 2331, 2705, 2774
DdeI	CTNAG	7 cut(s)	706, 858, 1878, 2188, 2427, 2469, 2675
DraI	TTTAAA	1 cut(s)	2895
EaeI	YGGCCR	4 cut(s)	172, 183, 229, 2569
Eam1104I	CTCTTC	1 cut(s)	807
EarI	CTCTTC	1 cut(s)	807
EciI	GGCGGA	1 cut(s)	233
Eco130I	CCWWGG	4 cut(s)	686, 864, 2057, 3259
Eco147I	AGGCCT	2 cut(s)	359, 3110
Eco47I	GGWCC	4 cut(s)	69, 690, 849, 1625
Eco47III	AGCGCT	1 cut(s)	1418
Eco88I	CYCGRG	2 cut(s)	760, 928
EcoNI	CCTNNNNNAGG	1 cut(s)	1877
EcoRII	CCWGG	3 cut(s)	1750, 2835, 3165
EcoT14I	CCWWGG	4 cut(s)	686, 864, 2057, 3259
EcoT22I	ATGCAT	1 cut(s)	2071
ErhI	CCWWGG	4 cut(s)	686, 864, 2057, 3259
FaqI	GGGAC	5 cut(s)	272, 332, 1054, 1307, 2818
FauI	CCCGC	3 cut(s)	171, 1178, 2962
Fnu4HI	GCNGC	4 cut(s)	1156, 2786, 2967, 3081
Fsp4HI	GCNGC	4 cut(s)	1156, 2786, 2967, 3081
FspBI	CTAG	6 cut(s)	699, 807, 1247, 1367, 1523, 1775
FspI	TGCGCA	1 cut(s)	495
GlaI	GCGC	5 cut(s)	102, 104, 314, 495, 1418
GluI	GCNGC	4 cut(s)	1156, 2786, 2967, 3081
GsaI	CCCAGC	1 cut(s)	879
GsuI	CTGGAG	2 cut(s)	1309, 2886
HaeII	RGCGCY	2 cut(s)	316, 1420
HapII	CCGG	9 cut(s)	119, 156, 186, 228, 316, 1257, 1339, 1560, 1585
HgaI	GACGC	4 cut(s)	385, 2576, 2921, 3194
HhaI	GCGC	5 cut(s)	103, 105, 315, 496, 1419
Hin6I	GCGC	5 cut(s)	101, 103, 313, 494, 1417
HinP1I	GCGC	5 cut(s)	101, 103, 313, 494, 1417
HindIII	AAGCTT	3 cut(s)	1544, 1553, 2897
HpaII	CCGG	9 cut(s)	119, 156, 186, 228, 316, 1257, 1339, 1560, 1585
HphI	GGTGA	5 cut(s)	199, 637, 1914, 2348, 2990
Hpy166II	GTNNAC	2 cut(s)	1015, 2774
Hpy188I	TCNGA	9 cut(s)	73, 297, 414, 991, 1782, 2088, 2470, 3229, 3290
Hpy8I	GTNNAC	2 cut(s)	1015, 2774
Hpy99I	CGWCG	4 cut(s)	326, 389, 1650, 1766
HpyCH4III	ACNGT	9 cut(s)	305, 835, 892, 966, 2149, 2251, 2557, 2878, 3066
HpyCH4IV	ACGT	9 cut(s)	126, 237, 324, 384, 1648, 2008, 2752, 3004, 3099
HpyF10VI	GCNNNNNNNGC	5 cut(s)	111, 555, 1232, 1552, 3035
HpyF3I	CTNAG	7 cut(s)	706, 858, 1878, 2188, 2427, 2469, 2675
HpySE526I	ACGT	9 cut(s)	126, 237, 324, 384, 1648, 2008, 2752, 3004, 3099
HspAI	GCGC	5 cut(s)	101, 103, 313, 494, 1417
Kpn2I	TCCGGA	1 cut(s)	1256
LguI	GCTCTTC	1 cut(s)	807
LmnI	GCTCC	4 cut(s)	872, 1066, 1345, 2530
Lsp1109I	GCAGC	3 cut(s)	1142, 2978, 3067
LweI	GCATC	7 cut(s)	1192, 1945, 2632, 2953, 3175, 3210, 3220
MaeI	CTAG	6 cut(s)	699, 807, 1247, 1367, 1523, 1775
MaeII	ACGT	9 cut(s)	126, 237, 324, 384, 1648, 2008, 2752, 3004, 3099
MfeI	CAATTG	4 cut(s)	95, 497, 636, 1426
MflI	RGATCY	4 cut(s)	646, 1292, 1685, 2563
MhlI	GDGCHC	1 cut(s)	1063
MlyI	GAGTC	5 cut(s)	292, 1777, 2209, 2474, 3023
MmeI	TCCRAC	2 cut(s)	2242, 2670
Mph1103I	ATGCAT	1 cut(s)	2071
MroI	TCCGGA	1 cut(s)	1256
MroXI	GAANNNNTTC	1 cut(s)	2478
MseI	TTAA	8 cut(s)	978, 1566, 2411, 2616, 2700, 2894, 2910, 3087
MslI	CAYNNNNRTG	3 cut(s)	1805, 2429, 2640
MspA1I	CMGCKG	3 cut(s)	1185, 2969, 3080
MspCI	CTTAAG	1 cut(s)	2615
MspI	CCGG	9 cut(s)	119, 156, 186, 228, 316, 1257, 1339, 1560, 1585
MspR9I	CCNGG	5 cut(s)	317, 1340, 1752, 2837, 3167
MunI	CAATTG	4 cut(s)	95, 497, 636, 1426
Mva1269I	GAATGC	2 cut(s)	2095, 2478
MvaI	CCWGG	3 cut(s)	1752, 2837, 3167
MvnI	CGCG	4 cut(s)	103, 379, 1766, 3042
MwoI	GCNNNNNNNGC	5 cut(s)	111, 555, 1232, 1552, 3035
NciI	CCSGG	2 cut(s)	317, 1340
NcoI	CCATGG	2 cut(s)	686, 3259
NlaIV	GGNNCC	8 cut(s)	190, 692, 850, 1395, 1687, 2078, 2256, 2406
NmuCI	GTSAC	4 cut(s)	373, 625, 2588, 2716
NruI	TCGCGA	1 cut(s)	3042
NsbI	TGCGCA	1 cut(s)	495
NsiI	ATGCAT	1 cut(s)	2071
NspI	RCATGY	1 cut(s)	2724
NspV	TTCGAA	2 cut(s)	87, 504
OliI	CACNNNNGTG	1 cut(s)	2429
PauI	GCGCGC	1 cut(s)	101
PceI	AGGCCT	2 cut(s)	359, 3110
PciI	ACATGT	1 cut(s)	2720
PciSI	GCTCTTC	1 cut(s)	807
PctI	GAATGC	2 cut(s)	2095, 2478
PdmI	GAANNNNTTC	1 cut(s)	2478
PfeI	GAWTC	8 cut(s)	213, 249, 473, 528, 785, 1170, 1532, 3119
PflFI	GACNNNGTC	1 cut(s)	1403
PflMI	CCANNNNNTGG	1 cut(s)	181
PkrI	GCNGC	4 cut(s)	1157, 2787, 2968, 3082
Ple19I	CGATCG	1 cut(s)	295
PleI	GAGTC	5 cut(s)	292, 1777, 2208, 2473, 3022
PpsI	GAGTC	5 cut(s)	292, 1777, 2208, 2473, 3022
Ppu21I	YACGTR	1 cut(s)	2753
PscI	ACATGT	1 cut(s)	2720
PsiI	TTATAA	2 cut(s)	522, 2373
Psp6I	CCWGG	3 cut(s)	1750, 2835, 3165
PspFI	CCCAGC	1 cut(s)	875
PspGI	CCWGG	3 cut(s)	1750, 2835, 3165
PspN4I	GGNNCC	8 cut(s)	190, 692, 850, 1395, 1687, 2078, 2256, 2406
PspPI	GGNCC	7 cut(s)	69, 112, 140, 690, 849, 1452, 1625
PsrI	GAACNNNNNNTAC	2 cut(s)	2688, 2720
PstNI	CAGNNNCTG	2 cut(s)	2762, 3233
PsuI	RGATCY	4 cut(s)	646, 1292, 1685, 2563
PsyI	GACNNNGTC	1 cut(s)	1403
PteI	GCGCGC	1 cut(s)	101
PvuI	CGATCG	1 cut(s)	295
PvuII	CAGCTG	1 cut(s)	3080
RruI	TCGCGA	1 cut(s)	3042
RsaI	GTAC	8 cut(s)	350, 383, 832, 2063, 2151, 2332, 2706, 2775
RsaNI	GTAC	8 cut(s)	349, 382, 831, 2062, 2150, 2331, 2705, 2774
RseI	CAYNNNNRTG	3 cut(s)	1805, 2429, 2640
SapI	GCTCTTC	1 cut(s)	807
SaqAI	TTAA	8 cut(s)	978, 1566, 2411, 2616, 2700, 2894, 2910, 3087
SatI	GCNGC	4 cut(s)	1156, 2786, 2967, 3081
Sau96I	GGNCC	7 cut(s)	69, 112, 140, 690, 849, 1452, 1625
ScaI	AGTACT	1 cut(s)	832
SchI	GAGTC	5 cut(s)	292, 1777, 2209, 2474, 3023
ScrFI	CCNGG	5 cut(s)	317, 1340, 1752, 2837, 3167
SduI	GDGCHC	1 cut(s)	1063
SfaNI	GCATC	7 cut(s)	1192, 1945, 2632, 2953, 3175, 3210, 3220
SfcI	CTRYAG	4 cut(s)	361, 834, 1312, 2488
SfuI	TTCGAA	2 cut(s)	87, 504
SinI	GGWCC	4 cut(s)	69, 690, 849, 1625
SmiMI	CAYNNNNRTG	3 cut(s)	1805, 2429, 2640
SmlI	CTYRAG	2 cut(s)	2603, 2615
SmoI	CTYRAG	2 cut(s)	2603, 2615
SpeI	ACTAGT	1 cut(s)	1522
SseBI	AGGCCT	2 cut(s)	359, 3110
SspI	AATATT	1 cut(s)	2293
SspMI	CTAG	6 cut(s)	699, 807, 1247, 1367, 1523, 1775
StuI	AGGCCT	2 cut(s)	359, 3110
StyD4I	CCNGG	5 cut(s)	315, 1338, 1750, 2835, 3165
StyI	CCWWGG	4 cut(s)	686, 864, 2057, 3259
TaaI	ACNGT	9 cut(s)	305, 835, 892, 966, 2149, 2251, 2557, 2878, 3066
TaiI	ACGT	9 cut(s)	129, 240, 327, 387, 1651, 2011, 2755, 3007, 3102
TatI	WGTACW	4 cut(s)	348, 830, 2149, 2773
TauI	GCSGC	1 cut(s)	2788
TfiI	GAWTC	8 cut(s)	213, 249, 473, 528, 785, 1170, 1532, 3119
Tru1I	TTAA	8 cut(s)	978, 1566, 2411, 2616, 2700, 2894, 2910, 3087
Tru9I	TTAA	8 cut(s)	978, 1566, 2411, 2616, 2700, 2894, 2910, 3087
TscAI	CASTG	8 cut(s)	556, 969, 1180, 1233, 2152, 2183, 2431, 2562
TseFI	GTSAC	4 cut(s)	373, 625, 2588, 2716
TseI	GCWGC	3 cut(s)	1155, 2966, 3080
Tsp45I	GTSAC	4 cut(s)	373, 625, 2588, 2716
TspDTI	ATGAA	7 cut(s)	891, 927, 1043, 2124, 2243, 2535, 2624
TspGWI	ACGGA	3 cut(s)	122, 135, 3085
TspRI	CASTG	8 cut(s)	556, 969, 1180, 1233, 2152, 2183, 2431, 2562
Tth111I	GACNNNGTC	1 cut(s)	1403
Van91I	CCANNNNNTGG	1 cut(s)	181
Vha464I	CTTAAG	1 cut(s)	2615
VpaK11BI	GGWCC	4 cut(s)	69, 690, 849, 1625
XagI	CCTNNNNNAGG	1 cut(s)	1877
XapI	RAATTY	4 cut(s)	574, 923, 1744, 2443
XceI	RCATGY	1 cut(s)	2724
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3266
XmnI	GAANNNNTTC	1 cut(s)	2478
XspI	CTAG	6 cut(s)	699, 807, 1247, 1367, 1523, 1775
ZrmI	AGTACT	1 cut(s)	832
Zsp2I	ATGCAT	1 cut(s)	2071

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.