FvH4_4g13130

Transcription elongation factor B polypeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013655
Physical Location & Seq
Forward (+)
16738516 .. 16738971
456 bp
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UTR
Exon/CDS
Intron
FvH4_4g13130.t1

Sequence Viewer

Length: 456 bp
ATGGACTTGGAACTTGTCGAACAGATCTTACCTCACTGTTCCAAGACGCAGTTGATCCATATAGAGAAGTCTACAAAAGACAGAGATCTGAGTCCGGTGACTGATAAGCTGTGGAAGACTTTCTATGAGAAGGAGTTCGGAATCGAATCCACCGAGTCGGTTTCGGAGATAATGAAAGAGTGGAATCTCAAGTTCAAATGGTCGGACTTGTACCAGGAGAAATCAAAGAGAGTGAAAGGGGCTTCGAAGAAAGAAAGTGATGGGAAGCTGAGCCGGCAAGTTAGGGTTAGCAAGAACCAGGTTCCACCACTTTCAAGAAGCAATAAAGGAAAGTGGCTAAAGAAGAAGTTCGGAGGGGCCAAACATTCAAGCTGTGGTAAAAGAAAAAAGGTGATGAGCTTAGAATATGCGAGGTTCGAAGGGGTGCATTATGTGAAAGCCCTTGAACCTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Elongin_A

Protein Analysis

152

Amino Acids

17.59

Weight (kDa)

9.72

Isoelectric Point (pI)

58.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Elongin_A PF06881 1 - 95 7.1e-15 RNA polymerase II transcription factor SIII (Elongin) subunit A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 71
AclWI GGATC 1 cut(s) 49
AfaI GTAC 1 cut(s) 212
AgsI TTSAA 4 cut(s) 196, 315, 369, 446
AjnI CCWGG 2 cut(s) 213, 297
AluBI AGCT 4 cut(s) 109, 268, 372, 399
AluI AGCT 4 cut(s) 109, 268, 372, 399
AlwI GGATC 1 cut(s) 49
AoxI GGCC 1 cut(s) 357
Asp700I GAANNNNTTC 3 cut(s) 119, 134, 347
AspS9I GGNCC 1 cut(s) 357
AsuHPI GGTGA 2 cut(s) 109, 403
AsuII TTCGAA 2 cut(s) 245, 417
BbsI GAAGAC 1 cut(s) 122
BccI CCATC 1 cut(s) 254
BciT130I CCWGG 2 cut(s) 215, 299
BglII AGATCT 2 cut(s) 24, 85
BlpI GCTNAGC 1 cut(s) 269
Bme1390I CCNGG 2 cut(s) 215, 299
BmgT120I GGNCC 1 cut(s) 357
BmiI GGNNCC 2 cut(s) 303, 358
BmrFI CCNGG 2 cut(s) 215, 299
BpiI GAAGAC 1 cut(s) 122
Bpu1102I GCTNAGC 1 cut(s) 269
Bpu14I TTCGAA 2 cut(s) 245, 417
BpuEI CTTGAG 1 cut(s) 173
BsaWI WCCGGW 1 cut(s) 94
Bse118I RCCGGY 1 cut(s) 273
BseBI CCWGG 2 cut(s) 215, 299
BseMII CTCAG 2 cut(s) 80, 260
BshFI GGCC 1 cut(s) 359
BsiSI CCGG 2 cut(s) 95, 274
BsnI GGCC 1 cut(s) 359
Bsp119I TTCGAA 2 cut(s) 245, 417
Bsp143I GATC 3 cut(s) 24, 54, 85
Bsp1720I GCTNAGC 1 cut(s) 269
BspANI GGCC 1 cut(s) 359
BspCNI CTCAG 2 cut(s) 81, 261
BspLI GGNNCC 2 cut(s) 303, 358
BspPI GGATC 1 cut(s) 49
BspT104I TTCGAA 2 cut(s) 245, 417
BsrFI RCCGGY 1 cut(s) 273
BssAI RCCGGY 1 cut(s) 273
BssMI GATC 3 cut(s) 24, 54, 85
Bst2UI CCWGG 2 cut(s) 215, 299
Bst4CI ACNGT 1 cut(s) 38
BstBI TTCGAA 2 cut(s) 245, 417
BstC8I GCNNGC 1 cut(s) 275
BstDEI CTNAG 3 cut(s) 89, 269, 400
BstKTI GATC 3 cut(s) 27, 57, 88
BstMBI GATC 3 cut(s) 24, 54, 85
BstMWI GCNNNNNNNGC 1 cut(s) 274
BstNI CCWGG 2 cut(s) 215, 299
BstSCI CCNGG 2 cut(s) 213, 297
BstV2I GAAGAC 1 cut(s) 122
BstX2I RGATCY 2 cut(s) 24, 85
BstYI RGATCY 2 cut(s) 24, 85
BsuRI GGCC 1 cut(s) 359
BtsIMutI CAGTG 1 cut(s) 34
Cac8I GCNNGC 1 cut(s) 275
Cfr10I RCCGGY 1 cut(s) 273
Cfr13I GGNCC 1 cut(s) 357
CseI GACGC 1 cut(s) 55
CsiI ACCWGGT 1 cut(s) 297
Csp6I GTAC 1 cut(s) 211
CviJI RGCY 9 cut(s) 109, 242, 268, 273, 337, 359, 372, 399, 440
CviKI_1 RGCY 9 cut(s) 109, 242, 268, 273, 337, 359, 372, 399, 440
CviQI GTAC 1 cut(s) 211
DdeI CTNAG 3 cut(s) 89, 269, 400
DpnI GATC 3 cut(s) 26, 56, 87
DpnII GATC 3 cut(s) 24, 54, 85
EcoRII CCWGG 2 cut(s) 213, 297
FaiI YATR 5 cut(s) 60, 62, 126, 408, 432
FblI GTMKAC 1 cut(s) 71
HaeIII GGCC 1 cut(s) 359
HapII CCGG 2 cut(s) 95, 274
HgaI GACGC 1 cut(s) 55
HinfI GANTC 5 cut(s) 91, 141, 146, 155, 184
HpaII CCGG 2 cut(s) 95, 274
HphI GGTGA 2 cut(s) 109, 403
Hpy166II GTNNAC 1 cut(s) 72
Hpy188I TCNGA 5 cut(s) 90, 140, 166, 205, 353
Hpy188III TCNNGA 1 cut(s) 315
Hpy8I GTNNAC 1 cut(s) 72
HpyAV CCTTC 2 cut(s) 124, 413
HpyCH4III ACNGT 1 cut(s) 38
HpyCH4V TGCA 1 cut(s) 427
HpyF10VI GCNNNNNNNGC 1 cut(s) 274
HpyF3I CTNAG 3 cut(s) 89, 269, 400
KroI GCCGGC 1 cut(s) 273
KroNI GCCGGC 1 cut(s) 275
Kzo9I GATC 3 cut(s) 24, 54, 85
LpnPI CCDG 6 cut(s) 108, 200, 227, 284, 287, 311
MabI ACCWGGT 1 cut(s) 297
MaeIII GTNAC 1 cut(s) 97
MalI GATC 3 cut(s) 26, 56, 87
MboI GATC 3 cut(s) 24, 54, 85
MboII GAAGA 3 cut(s) 127, 259, 355
MflI RGATCY 2 cut(s) 24, 85
MlyI GAGTC 2 cut(s) 100, 164
MmeI TCCRAC 1 cut(s) 183
MnlI CCTC 3 cut(s) 42, 347, 405
MroNI GCCGGC 1 cut(s) 273
MroXI GAANNNNTTC 3 cut(s) 119, 134, 347
MspI CCGG 2 cut(s) 95, 274
MspR9I CCNGG 2 cut(s) 215, 299
MvaI CCWGG 2 cut(s) 215, 299
MwoI GCNNNNNNNGC 1 cut(s) 274
NaeI GCCGGC 1 cut(s) 275
NdeII GATC 3 cut(s) 24, 54, 85
NgoMIV GCCGGC 1 cut(s) 273
NlaIV GGNNCC 2 cut(s) 303, 358
NmuCI GTSAC 1 cut(s) 97
NspV TTCGAA 2 cut(s) 245, 417
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PdiI GCCGGC 1 cut(s) 275
PdmI GAANNNNTTC 3 cut(s) 119, 134, 347
PfeI GAWTC 3 cut(s) 141, 146, 184
PleI GAGTC 2 cut(s) 99, 163
PpsI GAGTC 2 cut(s) 99, 163
Psp6I CCWGG 2 cut(s) 213, 297
PspGI CCWGG 2 cut(s) 213, 297
PspN4I GGNNCC 2 cut(s) 303, 358
PspPI GGNCC 1 cut(s) 357
PsrI GAACNNNNNNTAC 2 cut(s) 12, 44
PsuI RGATCY 2 cut(s) 24, 85
RsaI GTAC 1 cut(s) 212
RsaNI GTAC 1 cut(s) 211
Sau3AI GATC 3 cut(s) 24, 54, 85
Sau96I GGNCC 1 cut(s) 357
SchI GAGTC 2 cut(s) 100, 164
ScrFI CCNGG 2 cut(s) 215, 299
SetI ASST 9 cut(s) 34, 111, 270, 303, 374, 393, 401, 416, 451
SexAI ACCWGGT 1 cut(s) 297
SfuI TTCGAA 2 cut(s) 245, 417
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
StyD4I CCNGG 2 cut(s) 213, 297
TaaI ACNGT 1 cut(s) 38
TaqI TCGA 4 cut(s) 18, 144, 245, 417
TfiI GAWTC 3 cut(s) 141, 146, 184
TscAI CASTG 1 cut(s) 41
TseFI GTSAC 1 cut(s) 97
Tsp45I GTSAC 1 cut(s) 97
TspDTI ATGAA 1 cut(s) 188
TspRI CASTG 1 cut(s) 41
XmiI GTMKAC 1 cut(s) 71
XmnI GAANNNNTTC 3 cut(s) 119, 134, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.