Rw1G012740

Transcription elongation factor B polypeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0013655
Physical Location & Seq
Reverse (-)
28242097 .. 28242468
372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G012740.1

Sequence Viewer

Length: 372 bp
ATGGCCATAGATAATCGGATGTTTCTCGGCGATGTGAGCGACATGGACTTGGATCTTCTCGAGCAGATCTTACCTCACAGTTTCAAGTCGCAGTTGATCCATATAAAGAAGTCCACAAAAGACAGAGATCTGAGTCCAGTGACTAATAAGCTGTGGAAGAGCTTCTATGAGAAGATCGAATCAATCTATTCAGTTTCAGAGATAATGAAGGAGTGGAATCTCAAATTTAAGTGGTCGGAGTTGTACCAGGAGAAATCAAAAAGAGTGAAAGAGGCTGAGAAGAAAGAAGATGACAGGAAACTGAGCCAGCAAGTTAGGGTTTGCAAGAAGGTTCCACCATCTTCAAGCGACAAAACAAACAGGCCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Elongin_A

Protein Analysis

123

Amino Acids

14.61

Weight (kDa)

9.25

Isoelectric Point (pI)

48.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Elongin_A PF06881 10 - 106 7.8e-12 RNA polymerase II transcription factor SIII (Elongin) subunit A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 60, 91
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 224
AfaI GTAC 1 cut(s) 245
AgsI TTSAA 2 cut(s) 85, 345
AjnI CCWGG 1 cut(s) 246
AluBI AGCT 2 cut(s) 151, 162
AluI AGCT 2 cut(s) 151, 162
AlwI GGATC 2 cut(s) 60, 91
Ama87I CYCGRG 1 cut(s) 59
AoxI GGCC 2 cut(s) 3, 362
ApoI RAATTY 1 cut(s) 224
Asp700I GAANNNNTTC 1 cut(s) 161
AvaI CYCGRG 1 cut(s) 59
BalI TGGCCA 1 cut(s) 5
BccI CCATC 1 cut(s) 346
BciT130I CCWGG 1 cut(s) 248
BglII AGATCT 2 cut(s) 66, 127
Bme1390I CCNGG 1 cut(s) 248
BmeT110I CYCGRG 1 cut(s) 59
BmiI GGNNCC 1 cut(s) 333
BmrFI CCNGG 1 cut(s) 248
Bse1I ACTGG 1 cut(s) 137
BseBI CCWGG 1 cut(s) 248
BseGI GGATG 1 cut(s) 24
BseMII CTCAG 3 cut(s) 122, 267, 293
BseNI ACTGG 1 cut(s) 137
BshFI GGCC 2 cut(s) 5, 364
BsiHKCI CYCGRG 1 cut(s) 59
BsnI GGCC 2 cut(s) 5, 364
BsoBI CYCGRG 1 cut(s) 59
Bsp143I GATC 5 cut(s) 52, 66, 96, 127, 174
BspANI GGCC 2 cut(s) 5, 364
BspCNI CTCAG 3 cut(s) 123, 268, 294
BspLI GGNNCC 1 cut(s) 333
BspPI GGATC 2 cut(s) 60, 91
BspQI GCTCTTC 1 cut(s) 152
BsrI ACTGG 1 cut(s) 137
BssMI GATC 5 cut(s) 52, 66, 96, 127, 174
Bst2UI CCWGG 1 cut(s) 248
Bst4CI ACNGT 1 cut(s) 80
Bst6I CTCTTC 1 cut(s) 152
BstC8I GCNNGC 1 cut(s) 308
BstDEI CTNAG 3 cut(s) 131, 276, 302
BstF5I GGATG 1 cut(s) 24
BstKTI GATC 5 cut(s) 55, 69, 99, 130, 177
BstMBI GATC 5 cut(s) 52, 66, 96, 127, 174
BstMWI GCNNNNNNNGC 1 cut(s) 36
BstNI CCWGG 1 cut(s) 248
BstSCI CCNGG 1 cut(s) 246
BstX2I RGATCY 3 cut(s) 52, 66, 127
BstYI RGATCY 3 cut(s) 52, 66, 127
BsuRI GGCC 2 cut(s) 5, 364
BtgZI GCGATG 1 cut(s) 45
BtsCI GGATG 1 cut(s) 24
BtsIMutI CAGTG 1 cut(s) 144
Cac8I GCNNGC 1 cut(s) 308
Csp6I GTAC 1 cut(s) 244
CviAII CATG 1 cut(s) 43
CviJI RGCY 6 cut(s) 5, 151, 162, 275, 306, 364
CviKI_1 RGCY 6 cut(s) 5, 151, 162, 275, 306, 364
CviQI GTAC 1 cut(s) 244
DdeI CTNAG 3 cut(s) 131, 276, 302
DpnI GATC 5 cut(s) 54, 68, 98, 129, 176
DpnII GATC 5 cut(s) 52, 66, 96, 127, 174
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
Eco88I CYCGRG 1 cut(s) 59
EcoRII CCWGG 1 cut(s) 246
FaeI CATG 1 cut(s) 46
FaiI YATR 5 cut(s) 8, 44, 102, 104, 168
FatI CATG 1 cut(s) 42
FokI GGATG 1 cut(s) 31
HaeIII GGCC 2 cut(s) 5, 364
Hin1II CATG 1 cut(s) 46
HinfI GANTC 3 cut(s) 133, 179, 217
Hpy166II GTNNAC 1 cut(s) 114
Hpy188I TCNGA 4 cut(s) 18, 132, 199, 238
Hpy188III TCNNGA 1 cut(s) 59
Hpy8I GTNNAC 1 cut(s) 114
HpyAV CCTTC 2 cut(s) 202, 322
HpyCH4III ACNGT 1 cut(s) 80
HpyCH4V TGCA 1 cut(s) 324
HpyF10VI GCNNNNNNNGC 1 cut(s) 36
HpyF3I CTNAG 3 cut(s) 131, 276, 302
Hsp92II CATG 1 cut(s) 46
Kzo9I GATC 5 cut(s) 52, 66, 96, 127, 174
LguI GCTCTTC 1 cut(s) 152
LpnPI CCDG 6 cut(s) 150, 233, 260, 280, 320, 346
MaeIII GTNAC 1 cut(s) 139
MalI GATC 5 cut(s) 54, 68, 98, 129, 176
MboI GATC 5 cut(s) 52, 66, 96, 127, 174
MboII GAAGA 6 cut(s) 47, 169, 184, 292, 299, 333
MflI RGATCY 3 cut(s) 52, 66, 127
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 224, 367
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 142
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 2 cut(s) 84, 265
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 161
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 228, 370
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 248
MvaI CCWGG 1 cut(s) 248
MwoI GCNNNNNNNGC 1 cut(s) 36
NdeII GATC 5 cut(s) 52, 66, 96, 127, 174
NlaIII CATG 1 cut(s) 46
NlaIV GGNNCC 1 cut(s) 333
NmeAIII GCCGAG 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 139
PaeR7I CTCGAG 1 cut(s) 59
PciSI GCTCTTC 1 cut(s) 152
PdmI GAANNNNTTC 1 cut(s) 161
PfeI GAWTC 2 cut(s) 179, 217
PleI GAGTC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 246
PspGI CCWGG 1 cut(s) 246
PspN4I GGNNCC 1 cut(s) 333
PsuI RGATCY 3 cut(s) 52, 66, 127
RsaI GTAC 1 cut(s) 245
RsaNI GTAC 1 cut(s) 244
SapI GCTCTTC 1 cut(s) 152
SaqAI TTAA 2 cut(s) 228, 370
Sau3AI GATC 5 cut(s) 52, 66, 96, 127, 174
SchI GAGTC 1 cut(s) 142
ScrFI CCNGG 1 cut(s) 248
SetI ASST 4 cut(s) 76, 153, 164, 333
Sfr274I CTCGAG 1 cut(s) 59
SlaI CTCGAG 1 cut(s) 59
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 2 cut(s) 224, 367
StyD4I CCNGG 1 cut(s) 246
TaaI ACNGT 1 cut(s) 80
TaqI TCGA 2 cut(s) 60, 177
TasI AATT 2 cut(s) 224, 367
TfiI GAWTC 2 cut(s) 179, 217
Tru1I TTAA 2 cut(s) 228, 370
Tru9I TTAA 2 cut(s) 228, 370
TscAI CASTG 1 cut(s) 144
TseFI GTSAC 1 cut(s) 139
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 1 cut(s) 221
TspRI CASTG 1 cut(s) 144
XapI RAATTY 1 cut(s) 224
XhoI CTCGAG 1 cut(s) 59
XmnI GAANNNNTTC 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.