RchiOBHm_Chr1g0337481

Transcription elongation factor B polypeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0013655
Physical Location & Seq
Forward (+)
29232007 .. 29232432
426 bp
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UTR
Exon/CDS
Intron
PRQ56537

Sequence Viewer

Length: 426 bp
ATGTCTTTCGCTGACGTCAGTACGACCATAGATAATCAGAGGTTTCTGGTCAATGTGAACGACATGGACTTGGATCTTCTAGAGCAGATCTTACCTCACTGTTCCAAGACGCAGTTGATCCATATAGAGAAGTCCACAAAAGGCAGAGATCTGAGTCCGGTGATTGATAAGCTGTGGAAGAGTGCCTATGAGAAGGAGTTTGGAATCGAATCCACCAATTCGGTTTCGGAGATAATGAAGAAGTGGAATCTCACATTCAAGTGGTCGGAGTTGTACCAGGAGAAATCAAAGAGAGTAAAAAAGGCTGAGAAGAAAGAAGATGACAGGAAACGGAGCCGGCAAGTTAGGGTTTGCAAGAAGGTTCCACCATCTTCAAGCGATAAGAGAAACTGGCCAAAAGAGCAAGTTGATGAAGAAAGTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Elongin_A

Protein Analysis

141

Amino Acids

16.59

Weight (kDa)

8.91

Isoelectric Point (pI)

59.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Elongin_A PF06881 18 - 112 8.3e-15 RNA polymerase II transcription factor SIII (Elongin) subunit A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 18
AclWI GGATC 2 cut(s) 81, 112
AcoI YGGCCR 1 cut(s) 392
AcyI GRCGYC 1 cut(s) 15
AfaI GTAC 2 cut(s) 22, 275
AgsI TTSAA 2 cut(s) 259, 375
AjnI CCWGG 1 cut(s) 276
AluBI AGCT 1 cut(s) 172
AluI AGCT 1 cut(s) 172
AlwI GGATC 2 cut(s) 81, 112
AoxI GGCC 1 cut(s) 392
AsuHPI GGTGA 1 cut(s) 172
BalI TGGCCA 1 cut(s) 394
BccI CCATC 1 cut(s) 376
BciT130I CCWGG 1 cut(s) 278
BfaI CTAG 1 cut(s) 80
BglII AGATCT 2 cut(s) 87, 148
Bme1390I CCNGG 1 cut(s) 278
BmiI GGNNCC 2 cut(s) 335, 363
BmrFI CCNGG 1 cut(s) 278
BsaHI GRCGYC 1 cut(s) 15
BsaWI WCCGGW 1 cut(s) 157
Bse118I RCCGGY 1 cut(s) 336
Bse1I ACTGG 1 cut(s) 395
BseBI CCWGG 1 cut(s) 278
BseMII CTCAG 2 cut(s) 143, 297
BseNI ACTGG 1 cut(s) 395
BshFI GGCC 1 cut(s) 394
BsiSI CCGG 2 cut(s) 158, 337
BsnI GGCC 1 cut(s) 394
Bsp143I GATC 4 cut(s) 73, 87, 117, 148
BspANI GGCC 1 cut(s) 394
BspCNI CTCAG 2 cut(s) 144, 298
BspLI GGNNCC 2 cut(s) 335, 363
BspPI GGATC 2 cut(s) 81, 112
BsrFI RCCGGY 1 cut(s) 336
BsrI ACTGG 1 cut(s) 395
BssAI RCCGGY 1 cut(s) 336
BssMI GATC 4 cut(s) 73, 87, 117, 148
BssNI GRCGYC 1 cut(s) 15
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 1 cut(s) 101
Bst6I CTCTTC 1 cut(s) 173
BstACI GRCGYC 1 cut(s) 15
BstC8I GCNNGC 1 cut(s) 338
BstDEI CTNAG 2 cut(s) 152, 306
BstKTI GATC 4 cut(s) 76, 90, 120, 151
BstMBI GATC 4 cut(s) 73, 87, 117, 148
BstMWI GCNNNNNNNGC 1 cut(s) 400
BstNI CCWGG 1 cut(s) 278
BstSCI CCNGG 1 cut(s) 276
BstX2I RGATCY 3 cut(s) 73, 87, 148
BstYI RGATCY 3 cut(s) 73, 87, 148
BsuRI GGCC 1 cut(s) 394
BtsIMutI CAGTG 1 cut(s) 97
Cac8I GCNNGC 1 cut(s) 338
Cfr10I RCCGGY 1 cut(s) 336
CseI GACGC 1 cut(s) 118
Csp6I GTAC 2 cut(s) 21, 274
CviAII CATG 1 cut(s) 64
CviJI RGCY 4 cut(s) 172, 305, 336, 394
CviKI_1 RGCY 4 cut(s) 172, 305, 336, 394
CviQI GTAC 2 cut(s) 21, 274
DdeI CTNAG 2 cut(s) 152, 306
DpnI GATC 4 cut(s) 75, 89, 119, 150
DpnII GATC 4 cut(s) 73, 87, 117, 148
EaeI YGGCCR 1 cut(s) 392
Eam1104I CTCTTC 1 cut(s) 173
EarI CTCTTC 1 cut(s) 173
EcoRII CCWGG 1 cut(s) 276
FaeI CATG 1 cut(s) 67
FaiI YATR 5 cut(s) 29, 65, 123, 125, 189
FatI CATG 1 cut(s) 63
FspBI CTAG 1 cut(s) 80
HaeIII GGCC 1 cut(s) 394
HapII CCGG 2 cut(s) 158, 337
HgaI GACGC 1 cut(s) 118
Hin1I GRCGYC 1 cut(s) 15
Hin1II CATG 1 cut(s) 67
HinfI GANTC 4 cut(s) 154, 204, 209, 247
HpaII CCGG 2 cut(s) 158, 337
HphI GGTGA 1 cut(s) 172
Hpy166II GTNNAC 2 cut(s) 58, 135
Hpy188I TCNGA 4 cut(s) 39, 153, 229, 268
Hpy188III TCNNGA 1 cut(s) 80
Hpy8I GTNNAC 2 cut(s) 58, 135
HpyAV CCTTC 2 cut(s) 187, 352
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4IV ACGT 1 cut(s) 15
HpyCH4V TGCA 1 cut(s) 354
HpyF10VI GCNNNNNNNGC 1 cut(s) 400
HpyF3I CTNAG 2 cut(s) 152, 306
HpySE526I ACGT 1 cut(s) 15
Hsp92I GRCGYC 1 cut(s) 15
Hsp92II CATG 1 cut(s) 67
KroI GCCGGC 1 cut(s) 336
KroNI GCCGGC 1 cut(s) 338
Kzo9I GATC 4 cut(s) 73, 87, 117, 148
LmnI GCTCC 1 cut(s) 333
LpnPI CCDG 7 cut(s) 32, 171, 263, 290, 310, 350, 376
MaeI CTAG 1 cut(s) 80
MaeII ACGT 1 cut(s) 15
MalI GATC 4 cut(s) 75, 89, 119, 150
MboI GATC 4 cut(s) 73, 87, 117, 148
MboII GAAGA 7 cut(s) 68, 190, 250, 322, 329, 363, 425
MflI RGATCY 3 cut(s) 73, 87, 148
MlsI TGGCCA 1 cut(s) 394
MluCI AATT 1 cut(s) 217
MluNI TGGCCA 1 cut(s) 394
MlyI GAGTC 1 cut(s) 163
MmeI TCCRAC 1 cut(s) 246
MnlI CCTC 2 cut(s) 33, 105
Mox20I TGGCCA 1 cut(s) 394
MroNI GCCGGC 1 cut(s) 336
MscI TGGCCA 1 cut(s) 394
MseI TTAA 1 cut(s) 424
MslI CAYNNNNRTG 1 cut(s) 259
Msp20I TGGCCA 1 cut(s) 394
MspI CCGG 2 cut(s) 158, 337
MspR9I CCNGG 1 cut(s) 278
MvaI CCWGG 1 cut(s) 278
MwoI GCNNNNNNNGC 1 cut(s) 400
NaeI GCCGGC 1 cut(s) 338
NdeII GATC 4 cut(s) 73, 87, 117, 148
NgoMIV GCCGGC 1 cut(s) 336
NlaIII CATG 1 cut(s) 67
NlaIV GGNNCC 2 cut(s) 335, 363
PdiI GCCGGC 1 cut(s) 338
PfeI GAWTC 3 cut(s) 204, 209, 247
PleI GAGTC 1 cut(s) 162
PpsI GAGTC 1 cut(s) 162
Psp6I CCWGG 1 cut(s) 276
PspGI CCWGG 1 cut(s) 276
PspN4I GGNNCC 2 cut(s) 335, 363
PsuI RGATCY 3 cut(s) 73, 87, 148
RsaI GTAC 2 cut(s) 22, 275
RsaNI GTAC 2 cut(s) 21, 274
RseI CAYNNNNRTG 1 cut(s) 259
SaqAI TTAA 1 cut(s) 424
Sau3AI GATC 4 cut(s) 73, 87, 117, 148
SchI GAGTC 1 cut(s) 163
ScrFI CCNGG 1 cut(s) 278
SetI ASST 5 cut(s) 18, 44, 97, 174, 363
SmiMI CAYNNNNRTG 1 cut(s) 259
Sse9I AATT 1 cut(s) 217
SspMI CTAG 1 cut(s) 80
StyD4I CCNGG 1 cut(s) 276
TaaI ACNGT 1 cut(s) 101
TaiI ACGT 1 cut(s) 18
TaqI TCGA 1 cut(s) 207
TasI AATT 1 cut(s) 217
TfiI GAWTC 3 cut(s) 204, 209, 247
Tru1I TTAA 1 cut(s) 424
Tru9I TTAA 1 cut(s) 424
TscAI CASTG 1 cut(s) 104
TspDTI ATGAA 2 cut(s) 251, 426
TspGWI ACGGA 1 cut(s) 346
TspRI CASTG 1 cut(s) 104
XbaI TCTAGA 1 cut(s) 79
XspI CTAG 1 cut(s) 80
ZraI GACGTC 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.