FvH4_7g07290

Transcription elongation factor B polypeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0013655
Physical Location & Seq
Reverse (-)
7262780 .. 7263280
501 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g07290.t1

Sequence Viewer

Length: 501 bp
ATGGATATTCAAGCTAACACCGATTATCAGATCGTAAAGGCTCTCTCTCTCGCCGACGTCAGTACGGCCATAGAAAATCGGAGGTTTCTGGGGGATGTGAGCGACATGGACTTGGAACTTGTCGAACAGATCTTACCTCACTGTTCCAAGACACAGTTGATCCATATAGAGAAGTCCACAAAAAACAGAGATCTGAGTCCCGTGACTGATAAGCTGTGGAAGAGTTTCTATGAGAGGGAGTTCGGAACTGAATCGACCGAGTCGGTTTCACAGATAATGAAGGAGTGGAATCTCAAGTTCAAGTGGTCGGAGCTGTACCAGGAGAAATCAAAGAGAGTGAAAGGGGCTAAGAAGAAAGAAGATGACGGGAAACTGAGCCGGCAAGTGAGGGTTTGCAAGAAGGTTTCACCATTCTCAGGCAAGAAAGAAAAGTGGCTAAAGAAGAAGTTCGTAGGCGCCATTGTGCCAACTGCCAAGCATTCGAGCTGTGGTAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Elongin_A

Protein Analysis

167

Amino Acids

19.15

Weight (kDa)

9.55

Isoelectric Point (pI)

44.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Elongin_A PF06881 31 - 128 3.9e-18 RNA polymerase II transcription factor SIII (Elongin) subunit A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 60
AccB1I GGYRCC 1 cut(s) 455
AclWI GGATC 1 cut(s) 154
AcoI YGGCCR 1 cut(s) 66
AcyI GRCGYC 2 cut(s) 57, 456
AfaI GTAC 2 cut(s) 64, 317
AfiI CCNNNNNNNGG 1 cut(s) 416
AgsI TTSAA 2 cut(s) 11, 301
AjnI CCWGG 1 cut(s) 318
AluBI AGCT 4 cut(s) 14, 214, 313, 486
AluI AGCT 4 cut(s) 14, 214, 313, 486
AlwI GGATC 1 cut(s) 154
AoxI GGCC 1 cut(s) 66
Asp700I GAANNNNTTC 2 cut(s) 224, 446
AspLEI GCGC 1 cut(s) 458
AsuHPI GGTGA 1 cut(s) 399
BanI GGYRCC 1 cut(s) 455
BceAI ACGGC 1 cut(s) 81
BciT130I CCWGG 1 cut(s) 320
BfoI RGCGCY 1 cut(s) 459
BglII AGATCT 2 cut(s) 129, 190
Bme1390I CCNGG 1 cut(s) 320
BmiI GGNNCC 1 cut(s) 457
BmrFI CCNGG 1 cut(s) 320
BpuEI CTTGAG 1 cut(s) 278
BsaHI GRCGYC 2 cut(s) 57, 456
Bsc4I CCNNNNNNNGG 1 cut(s) 416
Bse118I RCCGGY 1 cut(s) 378
BseBI CCWGG 1 cut(s) 320
BseGI GGATG 1 cut(s) 100
BseLI CCNNNNNNNGG 1 cut(s) 416
BseMII CTCAG 3 cut(s) 185, 365, 429
Bsh1285I CGRYCG 1 cut(s) 258
BshFI GGCC 1 cut(s) 68
BshNI GGYRCC 1 cut(s) 455
BsiEI CGRYCG 1 cut(s) 258
BsiSI CCGG 1 cut(s) 379
BslFI GGGAC 1 cut(s) 183
BslI CCNNNNNNNGG 1 cut(s) 416
BsmFI GGGAC 1 cut(s) 183
BsmI GAATGC 1 cut(s) 478
BsnI GGCC 1 cut(s) 68
Bsp143I GATC 4 cut(s) 30, 129, 159, 190
BspANI GGCC 1 cut(s) 68
BspCNI CTCAG 3 cut(s) 186, 366, 428
BspLI GGNNCC 1 cut(s) 457
BspPI GGATC 1 cut(s) 154
BspT107I GGYRCC 1 cut(s) 455
BsrFI RCCGGY 1 cut(s) 378
BssAI RCCGGY 1 cut(s) 378
BssMI GATC 4 cut(s) 30, 129, 159, 190
BssNI GRCGYC 2 cut(s) 57, 456
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 2 cut(s) 143, 156
Bst6I CTCTTC 1 cut(s) 215
BstACI GRCGYC 2 cut(s) 57, 456
BstC8I GCNNGC 1 cut(s) 380
BstDEI CTNAG 4 cut(s) 194, 348, 374, 415
BstF5I GGATG 1 cut(s) 100
BstH2I RGCGCY 1 cut(s) 459
BstHHI GCGC 1 cut(s) 458
BstKTI GATC 4 cut(s) 33, 132, 162, 193
BstMBI GATC 4 cut(s) 30, 129, 159, 190
BstMCI CGRYCG 1 cut(s) 258
BstNI CCWGG 1 cut(s) 320
BstSCI CCNGG 1 cut(s) 318
BstX2I RGATCY 2 cut(s) 129, 190
BstYI RGATCY 2 cut(s) 129, 190
BsuRI GGCC 1 cut(s) 68
BtsCI GGATG 1 cut(s) 100
BtsIMutI CAGTG 1 cut(s) 139
Cac8I GCNNGC 1 cut(s) 380
CfoI GCGC 1 cut(s) 458
Cfr10I RCCGGY 1 cut(s) 378
Csp6I GTAC 2 cut(s) 63, 316
CviAII CATG 1 cut(s) 106
CviJI RGCY 9 cut(s) 14, 41, 68, 214, 313, 347, 378, 436, 486
CviKI_1 RGCY 9 cut(s) 14, 41, 68, 214, 313, 347, 378, 436, 486
CviQI GTAC 2 cut(s) 63, 316
DdeI CTNAG 4 cut(s) 194, 348, 374, 415
DinI GGCGCC 1 cut(s) 457
DpnI GATC 4 cut(s) 32, 131, 161, 192
DpnII GATC 4 cut(s) 30, 129, 159, 190
EaeI YGGCCR 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
EcoRII CCWGG 1 cut(s) 318
EgeI GGCGCC 1 cut(s) 457
EheI GGCGCC 1 cut(s) 457
FaeI CATG 1 cut(s) 109
FaiI YATR 5 cut(s) 71, 107, 165, 167, 231
FaqI GGGAC 1 cut(s) 183
FatI CATG 1 cut(s) 105
FokI GGATG 1 cut(s) 107
GlaI GCGC 1 cut(s) 457
HaeII RGCGCY 1 cut(s) 459
HaeIII GGCC 1 cut(s) 68
HapII CCGG 1 cut(s) 379
HhaI GCGC 1 cut(s) 458
Hin1I GRCGYC 2 cut(s) 57, 456
Hin1II CATG 1 cut(s) 109
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HinfI GANTC 4 cut(s) 196, 251, 260, 289
HpaII CCGG 1 cut(s) 379
HphI GGTGA 1 cut(s) 399
Hpy166II GTNNAC 1 cut(s) 177
Hpy188I TCNGA 5 cut(s) 30, 81, 195, 245, 310
Hpy8I GTNNAC 1 cut(s) 177
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 2 cut(s) 274, 394
HpyCH4III ACNGT 2 cut(s) 143, 156
HpyCH4IV ACGT 1 cut(s) 57
HpyCH4V TGCA 1 cut(s) 396
HpyF3I CTNAG 4 cut(s) 194, 348, 374, 415
HpySE526I ACGT 1 cut(s) 57
Hsp92I GRCGYC 2 cut(s) 57, 456
Hsp92II CATG 1 cut(s) 109
HspAI GCGC 1 cut(s) 456
KasI GGCGCC 1 cut(s) 455
KroI GCCGGC 1 cut(s) 378
KroNI GCCGGC 1 cut(s) 380
Kzo9I GATC 4 cut(s) 30, 129, 159, 190
LmnI GCTCC 1 cut(s) 310
LpnPI CCDG 5 cut(s) 74, 305, 332, 392, 402
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 1 cut(s) 202
MalI GATC 4 cut(s) 32, 131, 161, 192
MboI GATC 4 cut(s) 30, 129, 159, 190
MboII GAAGA 4 cut(s) 232, 364, 371, 454
MflI RGATCY 2 cut(s) 129, 190
Mly113I GGCGCC 1 cut(s) 456
MlyI GAGTC 2 cut(s) 205, 269
MmeI TCCRAC 1 cut(s) 288
MnlI CCTC 4 cut(s) 75, 147, 228, 381
MroNI GCCGGC 1 cut(s) 378
MroXI GAANNNNTTC 2 cut(s) 224, 446
MspI CCGG 1 cut(s) 379
MspR9I CCNGG 1 cut(s) 320
Mva1269I GAATGC 1 cut(s) 478
MvaI CCWGG 1 cut(s) 320
NaeI GCCGGC 1 cut(s) 380
NarI GGCGCC 1 cut(s) 456
NdeII GATC 4 cut(s) 30, 129, 159, 190
NgoMIV GCCGGC 1 cut(s) 378
NlaIII CATG 1 cut(s) 109
NlaIV GGNNCC 1 cut(s) 457
NmuCI GTSAC 1 cut(s) 202
PctI GAATGC 1 cut(s) 478
PdiI GCCGGC 1 cut(s) 380
PdmI GAANNNNTTC 2 cut(s) 224, 446
PfeI GAWTC 2 cut(s) 251, 289
PflFI GACNNNGTC 1 cut(s) 259
PleI GAGTC 2 cut(s) 204, 268
PluTI GGCGCC 1 cut(s) 459
PpsI GAGTC 2 cut(s) 204, 268
Psp6I CCWGG 1 cut(s) 318
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 1 cut(s) 457
PsrI GAACNNNNNNTAC 2 cut(s) 117, 149
PsuI RGATCY 2 cut(s) 129, 190
PsyI GACNNNGTC 1 cut(s) 259
RsaI GTAC 2 cut(s) 64, 317
RsaNI GTAC 2 cut(s) 63, 316
Sau3AI GATC 4 cut(s) 30, 129, 159, 190
SchI GAGTC 2 cut(s) 205, 269
ScrFI CCNGG 1 cut(s) 320
SetI ASST 8 cut(s) 16, 60, 86, 139, 216, 315, 405, 488
SfoI GGCGCC 1 cut(s) 457
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
SspDI GGCGCC 1 cut(s) 455
StyD4I CCNGG 1 cut(s) 318
TaaI ACNGT 2 cut(s) 143, 156
TaiI ACGT 1 cut(s) 60
TaqI TCGA 3 cut(s) 123, 254, 482
TaqII GACCGA 1 cut(s) 272
TfiI GAWTC 2 cut(s) 251, 289
TscAI CASTG 1 cut(s) 146
TseFI GTSAC 1 cut(s) 202
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 1 cut(s) 293
TspRI CASTG 1 cut(s) 146
Tth111I GACNNNGTC 1 cut(s) 259
XmnI GAANNNNTTC 2 cut(s) 224, 446
ZraI GACGTC 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.