FvH4_4g17690

Sucrose nonfermenting 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001327
Physical Location & Seq
Reverse (-)
21601607 .. 21608682
7076 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g17690.t7

Sequence Viewer

Length: 1476 bp
ATGTTTGCTACCAACATGGATGCGGCACGAGATATAGGTGGAGCTCAGAGCACAGTGTTGATCCCAATGCGGTTTGTGTGGCCTTATGGTGGCAGAAGTGTGTTTCTCAGTGGTTCATTTGCTCGCAGGTGGTCCGAGCTTGTTCCTATGACGCCGGTTGAGGGCTGCCCGACTGTGTTTCAGGCCATTTATAGCGTCACGCCTGGTATTCACCAGTACAAATTCTTTGTTGATGGGGAATGGCGACACGATGAGCACCAACCCTACGCAAATGGTGAATACGGGTTAGTGAACACCATGGTCTTGGCTACAGACCCTAATTTCTTTCAACCAAATGTACCTCCAGAGACGAGTCCTGGATCTCACATGGAAGAGGACACTGAGGCCTTTAAGCGTTTGGTCCAGATTACAGATGGTACATCAGATGTTGTACCAAGGATGTCAGAGGCTGATGTGCAGGGCTCGCATCATCGGATTTCTGTCTTCTTGTCCACTCACACCACTTACGAACTACTTCCGGAGTCGGGCAAGGTTGTTGCCTTGGACGTTGATTTACCTGTGAAGCAAGCATTTCACATATTGCATGAGCAGGGGATCCCTATGGCGCCACTTTGGGACTTCTGCAAGGGACAATTTGTTGGAGTTCTTAGTGCGTCGGATTTTATATTAATATTGAGAGAGCTTGGGAATCATGGGTCTAATCTGACTGAGGAAGAGCTTGAAACACATACTATAGCTGCTTGGAAAGAAGGAAAAGCATATCTGAATGGACAAATTGATGGTCATGGAAGAGCATTGCCACGACGTCTCGTCCATGCCGGGCCATATGATAATTTAAAAGATCTTGCTTTGAAATTTTTACAAAATGAGGTGGCTACGATTCCGATCATCCATTCATCTTCGGAGGATGGTTCATTTCCACAGCTATTACACCTTGCTTCACTGTCTGGTATTTTAAAATGTATTTGCAGATATTTTAGGCATTGTTCTAGCTCGTTGCCCATTCTCCAAGCACCAATATGTGCACTTCCTGTGGGTACTTGGGTTCCAGAAATCGGAGAGTCAAATCGTCGGCCATTAGCAATGTTGAGGCCAAGTGCGTCACTTAGTGCAGCTTTAAACTTGTTAGTTCAAGCTCAAGTAAGTTCAATACCTGTGGTGGATGATAATGACTCTTTGCTGGATATATATTGTCGGAGTGACATTACAGCTCTAGCTAAAGATAGAGCATACGCACATATTAATCTCAATGAAATGACCATTCAGCAGGCGTTGCAGTTGGGACAAGACTCGTACTCTCCTTTTGAGCAGAGAAGTCAGAGATGTCAGATGTGTTTACGCTCTGATTCACTTCATAAAGTGATGGAGAGATTAGCCAATCCAGGTGTCAGGCGACTTGTCATTGTTGAAGCTGGCAGTAAGCGAGTTGAAGGGATTGTTTCTCTAAGTGACGTTTTCAAGTTCTTGCTTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000266 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006833 GO:0006950 GO:0006996 GO:0007005 GO:0007031 GO:0007154 GO:0008047 GO:0008150 GO:0008152 GO:0009267 GO:0009605 GO:0009856 GO:0009859 GO:0009875 GO:0009893 GO:0009987 GO:0009991 GO:0010562 GO:0010604 GO:0016043 GO:0016301 GO:0016310 GO:0016559 GO:0016740 GO:0016772 GO:0016773 GO:0019207 GO:0019209 GO:0019220 GO:0019222 GO:0019538 GO:0019887 GO:0022414 GO:0030234 GO:0030295 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0031667 GO:0031668 GO:0031669 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0033554 GO:0033674 GO:0036211 GO:0042044 GO:0042149 GO:0042325 GO:0042327 GO:0042594 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044706 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048285 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051716 GO:0060255 GO:0065007 GO:0065009 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0098772 GO:0140096 GO:1901564 GO:2000377
KEGG Pathways
Metabolic & Signaling
ko04068 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 map04068 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410
Pfam Domains
Protein Families
AMPK1_CBM CBS

Protein Analysis

492

Amino Acids

54.4

Weight (kDa)

5.76

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBS PF00571 230 - 277 1.4e-06 CBS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 117
AatII GACGTC 1 cut(s) 808
Acc36I ACCTGC 1 cut(s) 117
AccB1I GGYRCC 1 cut(s) 604
AccIII TCCGGA 1 cut(s) 517
AciI CCGC 2 cut(s) 23, 70
AclWI GGATC 4 cut(s) 55, 367, 589, 602
AcoI YGGCCR 1 cut(s) 1073
AcsI RAATTY 2 cut(s) 221, 854
AcyI GRCGYC 3 cut(s) 152, 605, 805
AdeI CACNNNGTG 1 cut(s) 1109
AfaI GTAC 6 cut(s) 218, 339, 418, 432, 1039, 1295
AfiI CCNNNNNNNGG 4 cut(s) 89, 161, 524, 1055
AgsI TTSAA 8 cut(s) 329, 722, 853, 1133, 1149, 1409, 1430, 1459
AhdI GACNNNNNGTC 1 cut(s) 809
AjnI CCWGG 3 cut(s) 202, 355, 1381
Alw21I GWGCWC 4 cut(s) 46, 53, 258, 1027
Alw26I GTCTC 2 cut(s) 341, 812
Alw44I GTGCAC 1 cut(s) 1023
AlwI GGATC 4 cut(s) 55, 367, 589, 602
AlwNI CAGNNNCTG 1 cut(s) 449
Aor13HI TCCGGA 1 cut(s) 517
AoxI GGCC 6 cut(s) 80, 183, 384, 821, 1073, 1091
ApaLI GTGCAC 1 cut(s) 1023
ApeKI GCWGC 3 cut(s) 165, 737, 1112
ApoI RAATTY 2 cut(s) 221, 854
ArsI GACNNNNNNTTYG 2 cut(s) 766, 798
AseI ATTAAT 2 cut(s) 668, 1242
Asp700I GAANNNNTTC 1 cut(s) 513
AspLEI GCGC 1 cut(s) 607
AspS9I GGNCC 3 cut(s) 132, 400, 821
AsuC2I CCSGG 1 cut(s) 820
AsuHPI GGTGA 2 cut(s) 203, 287
AvaII GGWCC 2 cut(s) 132, 400
BaeGI GKGCMC 1 cut(s) 1027
BaeI ACNNNNGTAYC 2 cut(s) 1029, 1062
BamHI GGATCC 1 cut(s) 594
BanI GGYRCC 1 cut(s) 604
BanII GRGCYC 2 cut(s) 46, 464
BauI CACGAG 1 cut(s) 27
BbsI GAAGAC 1 cut(s) 475
Bbv12I GWGCWC 4 cut(s) 46, 53, 258, 1027
BbvI GCAGC 3 cut(s) 152, 724, 1124
BccI CCATC 5 cut(s) 227, 407, 773, 902, 1357
BciT130I CCWGG 3 cut(s) 204, 357, 1383
BcnI CCSGG 1 cut(s) 820
BcoDI GTCTC 2 cut(s) 341, 812
BfaI CTAG 2 cut(s) 990, 1214
BfmI CTRYAG 2 cut(s) 309, 732
BfoI RGCGCY 1 cut(s) 608
BfuAI ACCTGC 1 cut(s) 117
BglII AGATCT 1 cut(s) 841
BisI GCNGC 4 cut(s) 24, 166, 738, 1113
BlsI GCNGC 4 cut(s) 25, 167, 739, 1114
Bme1390I CCNGG 4 cut(s) 204, 357, 820, 1383
Bme18I GGWCC 2 cut(s) 132, 400
BmeRI GACNNNNNGTC 1 cut(s) 809
BmgT120I GGNCC 3 cut(s) 132, 400, 821
BmiI GGNNCC 3 cut(s) 596, 606, 1047
BmrFI CCNGG 4 cut(s) 204, 357, 820, 1383
BmsI GCATC 2 cut(s) 10, 475
BpiI GAAGAC 1 cut(s) 475
BpmI CTGGAG 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 1122
BpuMI CCSGG 1 cut(s) 820
BsaBI GATNNNNATC 1 cut(s) 884
BsaHI GRCGYC 3 cut(s) 152, 605, 805
BsaJI CCNNGG 3 cut(s) 297, 434, 540
BsaWI WCCGGW 1 cut(s) 517
Bsc4I CCNNNNNNNGG 4 cut(s) 89, 161, 524, 1055
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 1 cut(s) 214
Bse3DI GCAATG 2 cut(s) 794, 1089
Bse8I GATNNNNATC 1 cut(s) 884
BseAI TCCGGA 1 cut(s) 517
BseBI CCWGG 3 cut(s) 204, 357, 1383
BseDI CCNNGG 3 cut(s) 297, 434, 540
BseGI GGATG 5 cut(s) 25, 444, 888, 913, 1168
BseJI GATNNNNATC 1 cut(s) 884
BseLI CCNNNNNNNGG 4 cut(s) 89, 161, 524, 1055
BseMI GCAATG 2 cut(s) 794, 1089
BseMII CTCAG 4 cut(s) 59, 121, 372, 699
BseNI ACTGG 1 cut(s) 214
BseSI GKGCMC 1 cut(s) 1027
BseXI GCAGC 3 cut(s) 152, 724, 1124
BsgI GTGCAG 2 cut(s) 476, 1131
BshFI GGCC 6 cut(s) 82, 185, 386, 823, 1075, 1093
BshNI GGYRCC 1 cut(s) 604
BsiHKAI GWGCWC 4 cut(s) 46, 53, 258, 1027
BsiSI CCGG 3 cut(s) 155, 518, 819
BslFI GGGAC 3 cut(s) 629, 642, 1296
BslI CCNNNNNNNGG 4 cut(s) 89, 161, 524, 1055
BsmAI GTCTC 2 cut(s) 341, 812
BsmBI CGTCTC 2 cut(s) 341, 812
BsmFI GGGAC 3 cut(s) 629, 642, 1296
BsnI GGCC 6 cut(s) 82, 185, 386, 823, 1075, 1093
Bsp1286I GDGCHC 5 cut(s) 46, 53, 258, 464, 1027
Bsp13I TCCGGA 1 cut(s) 517
Bsp143I GATC 5 cut(s) 60, 359, 594, 841, 885
Bsp19I CCATGG 1 cut(s) 297
BspACI CCGC 2 cut(s) 23, 70
BspANI GGCC 6 cut(s) 82, 185, 386, 823, 1075, 1093
BspCNI CTCAG 4 cut(s) 58, 120, 373, 700
BspEI TCCGGA 1 cut(s) 517
BspLI GGNNCC 3 cut(s) 596, 606, 1047
BspMI ACCTGC 1 cut(s) 117
BspPI GGATC 4 cut(s) 55, 367, 589, 602
BspQI GCTCTTC 2 cut(s) 708, 784
BspT107I GGYRCC 1 cut(s) 604
BsrDI GCAATG 2 cut(s) 794, 1089
BsrFI RCCGGY 1 cut(s) 154
BsrI ACTGG 1 cut(s) 214
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 3 cut(s) 297, 434, 540
BssMI GATC 5 cut(s) 60, 359, 594, 841, 885
BssNI GRCGYC 3 cut(s) 152, 605, 805
BssSI CACGAG 1 cut(s) 27
BssT1I CCWWGG 3 cut(s) 297, 434, 540
Bst2BI CACGAG 1 cut(s) 27
Bst2UI CCWGG 3 cut(s) 204, 357, 1383
Bst4CI ACNGT 3 cut(s) 55, 175, 945
Bst6I CTCTTC 3 cut(s) 366, 708, 784
BstACI GRCGYC 3 cut(s) 152, 605, 805
BstAPI GCANNNNNTGC 1 cut(s) 1273
BstC8I GCNNGC 5 cut(s) 124, 464, 567, 1269, 1414
BstDEI CTNAG 7 cut(s) 45, 107, 381, 647, 708, 1106, 1445
BstDSI CCRYGG 1 cut(s) 297
BstF5I GGATG 5 cut(s) 25, 444, 888, 913, 1168
BstH2I RGCGCY 1 cut(s) 608
BstHHI GCGC 1 cut(s) 607
BstKTI GATC 5 cut(s) 63, 362, 597, 844, 888
BstMAI GTCTC 2 cut(s) 341, 812
BstMBI GATC 5 cut(s) 60, 359, 594, 841, 885
BstMWI GCNNNNNNNGC 2 cut(s) 463, 1273
BstNI CCWGG 3 cut(s) 204, 357, 1383
BstSCI CCNGG 4 cut(s) 202, 355, 818, 1381
BstSFI CTRYAG 2 cut(s) 309, 732
BstSLI GKGCMC 1 cut(s) 1027
BstV1I GCAGC 3 cut(s) 152, 724, 1124
BstV2I GAAGAC 1 cut(s) 475
BstX2I RGATCY 3 cut(s) 359, 594, 841
BstXI CCANNNNNNTGG 1 cut(s) 304
BstYI RGATCY 3 cut(s) 359, 594, 841
BsuRI GGCC 6 cut(s) 82, 185, 386, 823, 1075, 1093
BtgI CCRYGG 1 cut(s) 297
BtsCI GGATG 5 cut(s) 25, 444, 888, 913, 1168
BtsIMutI CAGTG 4 cut(s) 60, 115, 378, 941
BveI ACCTGC 1 cut(s) 117
Cac8I GCNNGC 5 cut(s) 124, 464, 567, 1269, 1414
CaiI CAGNNNCTG 1 cut(s) 449
CfoI GCGC 1 cut(s) 607
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 3 cut(s) 132, 400, 821
CseI GACGC 4 cut(s) 160, 184, 642, 1089
Csp6I GTAC 6 cut(s) 217, 338, 417, 431, 1038, 1294
CspCI CAANNNNNGTGG 2 cut(s) 1137, 1172
CviAII CATG 7 cut(s) 16, 298, 367, 584, 692, 785, 815
CviQI GTAC 6 cut(s) 217, 338, 417, 431, 1038, 1294
DdeI CTNAG 7 cut(s) 45, 107, 381, 647, 708, 1106, 1445
DinI GGCGCC 1 cut(s) 606
DpnI GATC 5 cut(s) 62, 361, 596, 843, 887
DpnII GATC 5 cut(s) 60, 359, 594, 841, 885
DraI TTTAAA 3 cut(s) 837, 957, 1119
DraIII CACNNNGTG 1 cut(s) 1109
DriI GACNNNNNGTC 1 cut(s) 809
EaeI YGGCCR 1 cut(s) 1073
Eam1104I CTCTTC 3 cut(s) 366, 708, 784
Eam1105I GACNNNNNGTC 1 cut(s) 809
EarI CTCTTC 3 cut(s) 366, 708, 784
Ecl136II GAGCTC 1 cut(s) 44
Eco130I CCWWGG 3 cut(s) 297, 434, 540
Eco147I AGGCCT 1 cut(s) 386
Eco24I GRGCYC 2 cut(s) 46, 464
Eco47I GGWCC 2 cut(s) 132, 400
Eco53kI GAGCTC 1 cut(s) 44
EcoICRI GAGCTC 1 cut(s) 44
EcoRII CCWGG 3 cut(s) 202, 355, 1381
EcoT14I CCWWGG 3 cut(s) 297, 434, 540
EcoT38I GRGCYC 2 cut(s) 46, 464
EgeI GGCGCC 1 cut(s) 606
EheI GGCGCC 1 cut(s) 606
ErhI CCWWGG 3 cut(s) 297, 434, 540
Esp3I CGTCTC 2 cut(s) 341, 812
FaeI CATG 7 cut(s) 19, 301, 370, 587, 695, 788, 818
FaqI GGGAC 3 cut(s) 629, 642, 1296
FatI CATG 7 cut(s) 15, 297, 366, 583, 691, 784, 814
FauNDI CATATG 1 cut(s) 826
Fnu4HI GCNGC 4 cut(s) 24, 166, 738, 1113
FokI GGATG 5 cut(s) 32, 451, 875, 920, 1175
FriOI GRGCYC 2 cut(s) 46, 464
Fsp4HI GCNGC 4 cut(s) 24, 166, 738, 1113
FspBI CTAG 2 cut(s) 990, 1214
GlaI GCGC 1 cut(s) 606
GluI GCNGC 4 cut(s) 24, 166, 738, 1113
GsuI CTGGAG 1 cut(s) 327
HaeII RGCGCY 1 cut(s) 608
HaeIII GGCC 6 cut(s) 82, 185, 386, 823, 1075, 1093
HapII CCGG 3 cut(s) 155, 518, 819
HgaI GACGC 4 cut(s) 160, 184, 642, 1089
HhaI GCGC 1 cut(s) 607
Hin1I GRCGYC 3 cut(s) 152, 605, 805
Hin1II CATG 7 cut(s) 19, 301, 370, 587, 695, 788, 818
Hin6I GCGC 1 cut(s) 605
HinP1I GCGC 1 cut(s) 605
HinfI GANTC 8 cut(s) 352, 521, 688, 880, 1061, 1172, 1289, 1346
HpaII CCGG 3 cut(s) 155, 518, 819
HphI GGTGA 2 cut(s) 203, 287
Hpy166II GTNNAC 4 cut(s) 292, 492, 1025, 1337
Hpy188III TCNNGA 4 cut(s) 344, 403, 518, 1049
Hpy8I GTNNAC 4 cut(s) 292, 492, 1025, 1337
Hpy99I CGWCG 3 cut(s) 658, 807, 1074
HpyAV CCTTC 2 cut(s) 743, 1424
HpyCH4III ACNGT 3 cut(s) 55, 175, 945
HpyCH4IV ACGT 3 cut(s) 546, 805, 1452
HpyCH4V TGCA 7 cut(s) 457, 583, 624, 969, 1025, 1112, 1276
HpyF10VI GCNNNNNNNGC 2 cut(s) 463, 1273
HpyF3I CTNAG 7 cut(s) 45, 107, 381, 647, 708, 1106, 1445
HpySE526I ACGT 3 cut(s) 546, 805, 1452
Hsp92I GRCGYC 3 cut(s) 152, 605, 805
Hsp92II CATG 7 cut(s) 19, 301, 370, 587, 695, 788, 818
HspAI GCGC 1 cut(s) 605
KasI GGCGCC 1 cut(s) 604
Kpn2I TCCGGA 1 cut(s) 517
Kzo9I GATC 5 cut(s) 60, 359, 594, 841, 885
LguI GCTCTTC 2 cut(s) 708, 784
LmnI GCTCC 1 cut(s) 41
Lsp1109I GCAGC 3 cut(s) 152, 724, 1124
LweI GCATC 2 cut(s) 10, 475
MaeI CTAG 2 cut(s) 990, 1214
MaeII ACGT 3 cut(s) 546, 805, 1452
MaeIII GTNAC 4 cut(s) 196, 1101, 1199, 1448
MalI GATC 5 cut(s) 62, 361, 596, 843, 887
MboI GATC 5 cut(s) 60, 359, 594, 841, 885
MboII GAAGA 5 cut(s) 383, 475, 725, 801, 891
MflI RGATCY 3 cut(s) 359, 594, 841
MhlI GDGCHC 5 cut(s) 46, 53, 258, 464, 1027
MluCI AATT 6 cut(s) 221, 319, 632, 774, 832, 854
Mly113I GGCGCC 1 cut(s) 605
MlyI GAGTC 5 cut(s) 361, 530, 1070, 1166, 1283
MmeI TCCRAC 3 cut(s) 619, 636, 1175
MnlI CCTC 9 cut(s) 154, 351, 367, 376, 439, 703, 862, 898, 1083
MroI TCCGGA 1 cut(s) 517
MroXI GAANNNNTTC 1 cut(s) 513
MseI TTAA 6 cut(s) 390, 668, 836, 956, 1118, 1242
MslI CAYNNNNRTG 1 cut(s) 1018
MspI CCGG 3 cut(s) 155, 518, 819
MspR9I CCNGG 4 cut(s) 204, 357, 820, 1383
MvaI CCWGG 3 cut(s) 204, 357, 1383
MwoI GCNNNNNNNGC 2 cut(s) 463, 1273
NarI GGCGCC 1 cut(s) 605
NciI CCSGG 1 cut(s) 820
NcoI CCATGG 1 cut(s) 297
NdeI CATATG 1 cut(s) 826
NdeII GATC 5 cut(s) 60, 359, 594, 841, 885
NlaIII CATG 7 cut(s) 19, 301, 370, 587, 695, 788, 818
NlaIV GGNNCC 3 cut(s) 596, 606, 1047
NmuCI GTSAC 4 cut(s) 196, 1101, 1199, 1448
PaqCI CACCTGC 1 cut(s) 117
PceI AGGCCT 1 cut(s) 386
PciSI GCTCTTC 2 cut(s) 708, 784
PdmI GAANNNNTTC 1 cut(s) 513
PfeI GAWTC 3 cut(s) 688, 880, 1346
PfoI TCCNGGA 1 cut(s) 355
PkrI GCNGC 4 cut(s) 25, 167, 739, 1114
PleI GAGTC 5 cut(s) 360, 529, 1069, 1166, 1283
PluTI GGCGCC 1 cut(s) 608
PpsI GAGTC 5 cut(s) 360, 529, 1069, 1166, 1283
PshBI ATTAAT 2 cut(s) 668, 1242
Psp124BI GAGCTC 1 cut(s) 46
Psp6I CCWGG 3 cut(s) 202, 355, 1381
PspGI CCWGG 3 cut(s) 202, 355, 1381
PspN4I GGNNCC 3 cut(s) 596, 606, 1047
PspPI GGNCC 3 cut(s) 132, 400, 821
PstNI CAGNNNCTG 1 cut(s) 449
PsuI RGATCY 3 cut(s) 359, 594, 841
RsaI GTAC 6 cut(s) 218, 339, 418, 432, 1039, 1295
RsaNI GTAC 6 cut(s) 217, 338, 417, 431, 1038, 1294
RseI CAYNNNNRTG 1 cut(s) 1018
SacI GAGCTC 1 cut(s) 46
SapI GCTCTTC 2 cut(s) 708, 784
SaqAI TTAA 6 cut(s) 390, 668, 836, 956, 1118, 1242
SatI GCNGC 4 cut(s) 24, 166, 738, 1113
Sau3AI GATC 5 cut(s) 60, 359, 594, 841, 885
Sau96I GGNCC 3 cut(s) 132, 400, 821
SchI GAGTC 5 cut(s) 361, 530, 1070, 1166, 1283
ScrFI CCNGG 4 cut(s) 204, 357, 820, 1383
SduI GDGCHC 5 cut(s) 46, 53, 258, 464, 1027
SfaNI GCATC 2 cut(s) 10, 475
SfcI CTRYAG 2 cut(s) 309, 732
SfoI GGCGCC 1 cut(s) 606
SinI GGWCC 2 cut(s) 132, 400
SmiMI CAYNNNNRTG 1 cut(s) 1018
SmlI CTYRAG 1 cut(s) 1137
SmoI CTYRAG 1 cut(s) 1137
Sse9I AATT 6 cut(s) 221, 319, 632, 774, 832, 854
SseBI AGGCCT 1 cut(s) 386
SsiI CCGC 2 cut(s) 23, 70
SspDI GGCGCC 1 cut(s) 604
SspI AATATT 1 cut(s) 672
SspMI CTAG 2 cut(s) 990, 1214
SstI GAGCTC 1 cut(s) 46
StuI AGGCCT 1 cut(s) 386
StyD4I CCNGG 4 cut(s) 202, 355, 818, 1381
StyI CCWWGG 3 cut(s) 297, 434, 540
TaaI ACNGT 3 cut(s) 55, 175, 945
TaiI ACGT 3 cut(s) 549, 808, 1455
TasI AATT 6 cut(s) 221, 319, 632, 774, 832, 854
TatI WGTACW 1 cut(s) 216
TauI GCSGC 1 cut(s) 26
TfiI GAWTC 3 cut(s) 688, 880, 1346
Tru1I TTAA 6 cut(s) 390, 668, 836, 956, 1118, 1242
Tru9I TTAA 6 cut(s) 390, 668, 836, 956, 1118, 1242
TscAI CASTG 4 cut(s) 60, 115, 385, 948
TseFI GTSAC 4 cut(s) 196, 1101, 1199, 1448
TseI GCWGC 3 cut(s) 165, 737, 1112
Tsp45I GTSAC 4 cut(s) 196, 1101, 1199, 1448
TspDTI ATGAA 5 cut(s) 105, 885, 903, 1266, 1343
TspRI CASTG 4 cut(s) 60, 115, 385, 948
VneI GTGCAC 1 cut(s) 1023
VpaK11BI GGWCC 2 cut(s) 132, 400
VspI ATTAAT 2 cut(s) 668, 1242
XapI RAATTY 2 cut(s) 221, 854
XcmI CCANNNNNNNNNTGG 1 cut(s) 410
XmnI GAANNNNTTC 1 cut(s) 513
XspI CTAG 2 cut(s) 990, 1214
ZraI GACGTC 1 cut(s) 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.