Rh3CG065700

Sucrose nonfermenting 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0001327
Physical Location & Seq
Reverse (-)
4538902 .. 4545183
6282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG065700.1

Sequence Viewer

Length: 1008 bp
ATGAAGAAGTCTTGTGCTGATATTGGGATCTGTTGTTTGTTTCAGTACAAGTTCTTGGTTGACGGAGAATGGCGACATAACGAGCAACTGCCTTTTGTCACTGGGAATTGTGGAATAGTGAATACCGTCTTTCTAACTGGGGAGCCAGATACAGTTCCTACTAGTTTCAGTCCTGAGACTTCTGGCAGATCCAATATGGATGTAGATAACGAAGTCTTTACGCGTATGGAAGTAGCTCCAAGGATCTCAGCAGCTGAATTGGAGATCTCTCAGCAGCGTATATCAGTATTTCTATCCACACATACTGCTTATGAGTTGCTTCCTGAGTCAGGCAAGGTCATTGCCTTGGATGTCAATTTACCAGTGAAAGAAGCATTCCATATCCTACACGAACAGGGAGTACCTGTGGCTCCTCTCTGGGATTTTGAAAAGGGACAATTTGTAGGAGTTCTTAGCGCATTGGATTTCATCTTAATATTGAGAGAGCTTGGGAATCATGGGTCAAATTTGACTGGGGAAGAGCTGGAGACTCATACTATAGCAGCATGGAAAGAGGGAAAATTAAATTTGAACAGACAACTTGATGGAAATGGGAGATGCTATCCGCCACATTTGATCTCTGCCGGGCCTTATGATTCTCTCAAAGAAGTTGCTGTCAGAATTCTGCAAAACAAGGTGGCCACAGTTCCAATTCTTCATACTTCCTCAGAGGATGGTTCATTTCCACAGCTACTGCATCTTGCCTCCCTTTCGGGAATATTAAAATGCATATGCAGGCATTTCAGACATTCTTCTACCTCCTTGCCAATACTCCAACATCCTATTTCAGAATTCCGTATAGGTACATGGGTTCCAAATATTGGGGAGTCAAATGGAAGGCTGCTGGCCATGTTGAGACCAAATTCATCTCTTGCTGATGCCCTGTCTTTGTTGGTACAAGCTGAAGTTAGTTCAATACCCATAGTGGATGACAATGATTCATTGCTGGATGTTTATTCACGAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000266 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006833 GO:0006950 GO:0006996 GO:0007005 GO:0007031 GO:0007154 GO:0008047 GO:0008150 GO:0008152 GO:0009267 GO:0009605 GO:0009856 GO:0009859 GO:0009875 GO:0009893 GO:0009987 GO:0009991 GO:0010562 GO:0010604 GO:0016043 GO:0016301 GO:0016310 GO:0016559 GO:0016740 GO:0016772 GO:0016773 GO:0019207 GO:0019209 GO:0019220 GO:0019222 GO:0019538 GO:0019887 GO:0022414 GO:0030234 GO:0030295 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0031667 GO:0031668 GO:0031669 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0033554 GO:0033674 GO:0036211 GO:0042044 GO:0042149 GO:0042325 GO:0042327 GO:0042594 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044706 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048285 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051716 GO:0060255 GO:0065007 GO:0065009 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0098772 GO:0140096 GO:1901564 GO:2000377
KEGG Pathways
Metabolic & Signaling
ko04068 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 map04068 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410
Pfam Domains
Protein Families
AMPK1_CBM CBS

Protein Analysis

335

Amino Acids

36.97

Weight (kDa)

5.45

Isoelectric Point (pI)

38.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMPK1_CBM PF16561 14 - 45 7e-07 Glycogen recognition site of AMP-activated protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 860
AccII CGCG 1 cut(s) 223
AciI CCGC 1 cut(s) 605
AclWI GGATC 3 cut(s) 35, 183, 251
AcoI YGGCCR 2 cut(s) 678, 885
AcsI RAATTY 5 cut(s) 505, 565, 660, 830, 901
AcuI CTGAAG 1 cut(s) 963
AfaI GTAC 4 cut(s) 47, 402, 844, 936
AfiI CCNNNNNNNGG 2 cut(s) 329, 860
AflIII ACRYGT 1 cut(s) 221
AgsI TTSAA 3 cut(s) 428, 571, 954
AhlI ACTAGT 1 cut(s) 161
AleI CACNNNNGTG 1 cut(s) 1003
AluBI AGCT 6 cut(s) 236, 254, 487, 523, 730, 941
AluI AGCT 6 cut(s) 236, 254, 487, 523, 730, 941
Alw26I GTCTC 3 cut(s) 170, 521, 889
AlwI GGATC 3 cut(s) 35, 183, 251
AlwNI CAGNNNCTG 2 cut(s) 254, 733
AoxI GGCC 3 cut(s) 626, 678, 885
ApeKI GCWGC 4 cut(s) 251, 274, 542, 880
ApoI RAATTY 5 cut(s) 505, 565, 660, 830, 901
AspLEI GCGC 1 cut(s) 458
AspS9I GGNCC 1 cut(s) 626
AsuC2I CCSGG 1 cut(s) 625
BaeI ACNNNNGTAYC 2 cut(s) 834, 867
BalI TGGCCA 2 cut(s) 680, 887
BbvI GCAGC 4 cut(s) 263, 286, 554, 867
BccI CCATC 2 cut(s) 578, 707
BcnI CCSGG 1 cut(s) 625
BcoDI GTCTC 3 cut(s) 170, 521, 889
BcuI ACTAGT 1 cut(s) 161
BfaI CTAG 1 cut(s) 162
BfmI CTRYAG 1 cut(s) 537
BglII AGATCT 1 cut(s) 264
BisI GCNGC 4 cut(s) 252, 275, 543, 881
BlsI GCNGC 4 cut(s) 253, 276, 544, 882
Bme1390I CCNGG 1 cut(s) 625
BmgT120I GGNCC 1 cut(s) 626
BmiI GGNNCC 3 cut(s) 144, 411, 852
BmrFI CCNGG 1 cut(s) 625
BmrI ACTGGG 3 cut(s) 111, 147, 522
BmsI GCATC 3 cut(s) 587, 745, 907
BmuI ACTGGG 3 cut(s) 111, 147, 522
BpmI CTGGAG 1 cut(s) 545
BpuMI CCSGG 1 cut(s) 625
BsaI GGTCTC 1 cut(s) 889
BsaJI CCNNGG 2 cut(s) 239, 345
BsaXI ACNNNNNCTCC 2 cut(s) 390, 420
Bsc4I CCNNNNNNNGG 2 cut(s) 329, 860
Bse1I ACTGG 4 cut(s) 106, 142, 362, 517
Bse3DI GCAATG 2 cut(s) 339, 980
BseDI CCNNGG 2 cut(s) 239, 345
BseGI GGATG 6 cut(s) 205, 355, 718, 817, 973, 994
BseLI CCNNNNNNNGG 2 cut(s) 329, 860
BseMI GCAATG 2 cut(s) 339, 980
BseMII CTCAG 5 cut(s) 165, 261, 284, 315, 720
BseNI ACTGG 4 cut(s) 106, 142, 362, 517
BseRI GAGGAG 1 cut(s) 402
BseXI GCAGC 4 cut(s) 263, 286, 554, 867
Bsh1236I CGCG 1 cut(s) 223
BshFI GGCC 3 cut(s) 628, 680, 887
BsiSI CCGG 1 cut(s) 624
BslFI GGGAC 1 cut(s) 447
BslI CCNNNNNNNGG 2 cut(s) 329, 860
BsmAI GTCTC 3 cut(s) 170, 521, 889
BsmFI GGGAC 1 cut(s) 447
BsmI GAATGC 1 cut(s) 374
BsnI GGCC 3 cut(s) 628, 680, 887
Bso31I GGTCTC 1 cut(s) 889
Bsp143I GATC 5 cut(s) 27, 188, 243, 264, 615
BspACI CCGC 1 cut(s) 605
BspANI GGCC 3 cut(s) 628, 680, 887
BspCNI CTCAG 5 cut(s) 166, 260, 283, 316, 719
BspFNI CGCG 1 cut(s) 223
BspLI GGNNCC 3 cut(s) 144, 411, 852
BspPI GGATC 3 cut(s) 35, 183, 251
BspQI GCTCTTC 1 cut(s) 513
BspTNI GGTCTC 1 cut(s) 889
BsrDI GCAATG 2 cut(s) 339, 980
BsrI ACTGG 4 cut(s) 106, 142, 362, 517
BssECI CCNNGG 2 cut(s) 239, 345
BssMI GATC 5 cut(s) 27, 188, 243, 264, 615
BssT1I CCWWGG 2 cut(s) 239, 345
Bst4CI ACNGT 3 cut(s) 127, 154, 685
Bst6I CTCTTC 1 cut(s) 513
BstC8I GCNNGC 2 cut(s) 776, 885
BstDEI CTNAG 6 cut(s) 174, 247, 270, 324, 452, 706
BstENI CCTNNNNNAGG 1 cut(s) 327
BstF5I GGATG 6 cut(s) 205, 355, 718, 817, 973, 994
BstFNI CGCG 1 cut(s) 223
BstHHI GCGC 1 cut(s) 458
BstKTI GATC 5 cut(s) 30, 191, 246, 267, 618
BstMAI GTCTC 3 cut(s) 170, 521, 889
BstMBI GATC 5 cut(s) 27, 188, 243, 264, 615
BstSCI CCNGG 1 cut(s) 623
BstSFI CTRYAG 1 cut(s) 537
BstUI CGCG 1 cut(s) 223
BstV1I GCAGC 4 cut(s) 263, 286, 554, 867
BstX2I RGATCY 4 cut(s) 27, 188, 243, 264
BstYI RGATCY 4 cut(s) 27, 188, 243, 264
BsuRI GGCC 3 cut(s) 628, 680, 887
BtsCI GGATG 6 cut(s) 205, 355, 718, 817, 973, 994
BtsIMutI CAGTG 2 cut(s) 99, 369
Cac8I GCNNGC 2 cut(s) 776, 885
CaiI CAGNNNCTG 2 cut(s) 254, 733
CfoI GCGC 1 cut(s) 458
Cfr13I GGNCC 1 cut(s) 626
Csp6I GTAC 4 cut(s) 46, 401, 843, 935
CviAII CATG 4 cut(s) 497, 546, 846, 889
CviQI GTAC 4 cut(s) 46, 401, 843, 935
DdeI CTNAG 6 cut(s) 174, 247, 270, 324, 452, 706
DpnI GATC 5 cut(s) 29, 190, 245, 266, 617
DpnII GATC 5 cut(s) 27, 188, 243, 264, 615
EaeI YGGCCR 2 cut(s) 678, 885
Eam1104I CTCTTC 1 cut(s) 513
EarI CTCTTC 1 cut(s) 513
EciI GGCGGA 1 cut(s) 594
Eco130I CCWWGG 2 cut(s) 239, 345
Eco31I GGTCTC 1 cut(s) 889
Eco57I CTGAAG 1 cut(s) 963
EcoNI CCTNNNNNAGG 1 cut(s) 327
EcoRI GAATTC 2 cut(s) 660, 830
EcoT14I CCWWGG 2 cut(s) 239, 345
EcoT22I ATGCAT 1 cut(s) 770
ErhI CCWWGG 2 cut(s) 239, 345
FaeI CATG 4 cut(s) 500, 549, 849, 892
FaqI GGGAC 1 cut(s) 447
FatI CATG 4 cut(s) 496, 545, 845, 888
FauNDI CATATG 1 cut(s) 770
Fnu4HI GCNGC 4 cut(s) 252, 275, 543, 881
FokI GGATG 6 cut(s) 212, 362, 725, 804, 980, 1001
Fsp4HI GCNGC 4 cut(s) 252, 275, 543, 881
FspBI CTAG 1 cut(s) 162
GlaI GCGC 1 cut(s) 457
GluI GCNGC 4 cut(s) 252, 275, 543, 881
GsuI CTGGAG 1 cut(s) 545
HaeIII GGCC 3 cut(s) 628, 680, 887
HapII CCGG 1 cut(s) 624
HhaI GCGC 1 cut(s) 458
Hin1II CATG 4 cut(s) 500, 549, 849, 892
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HinfI GANTC 6 cut(s) 326, 493, 529, 635, 866, 977
HpaII CCGG 1 cut(s) 624
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 4 cut(s) 659, 709, 785, 829
Hpy188III TCNNGA 4 cut(s) 173, 323, 753, 999
Hpy8I GTNNAC 1 cut(s) 61
HpyAV CCTTC 2 cut(s) 870, 996
HpyCH4III ACNGT 3 cut(s) 127, 154, 685
HpyCH4V TGCA 4 cut(s) 667, 736, 768, 774
HpyF3I CTNAG 6 cut(s) 174, 247, 270, 324, 452, 706
Hsp92II CATG 4 cut(s) 500, 549, 849, 892
HspAI GCGC 1 cut(s) 456
Kzo9I GATC 5 cut(s) 27, 188, 243, 264, 615
LguI GCTCTTC 1 cut(s) 513
LmnI GCTCC 3 cut(s) 142, 241, 415
Lsp1109I GCAGC 4 cut(s) 263, 286, 554, 867
LweI GCATC 3 cut(s) 587, 745, 907
MaeI CTAG 1 cut(s) 162
MaeIII GTNAC 1 cut(s) 97
MalI GATC 5 cut(s) 29, 190, 245, 266, 617
MboI GATC 5 cut(s) 27, 188, 243, 264, 615
MboII GAAGA 4 cut(s) 16, 530, 686, 783
MflI RGATCY 4 cut(s) 27, 188, 243, 264
MlsI TGGCCA 2 cut(s) 680, 887
MluI ACGCGT 1 cut(s) 221
MluNI TGGCCA 2 cut(s) 680, 887
MlyI GAGTC 3 cut(s) 335, 523, 875
MmeI TCCRAC 1 cut(s) 838
MnlI CCTC 6 cut(s) 423, 547, 703, 715, 754, 808
Mox20I TGGCCA 2 cut(s) 680, 887
Mph1103I ATGCAT 1 cut(s) 770
MscI TGGCCA 2 cut(s) 680, 887
MseI TTAA 3 cut(s) 473, 563, 761
MslI CAYNNNNRTG 1 cut(s) 1003
Msp20I TGGCCA 2 cut(s) 680, 887
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 1 cut(s) 624
MspR9I CCNGG 1 cut(s) 625
Mva1269I GAATGC 1 cut(s) 374
MvnI CGCG 1 cut(s) 223
NciI CCSGG 1 cut(s) 625
NdeI CATATG 1 cut(s) 770
NdeII GATC 5 cut(s) 27, 188, 243, 264, 615
NlaIII CATG 4 cut(s) 500, 549, 849, 892
NlaIV GGNNCC 3 cut(s) 144, 411, 852
NmuCI GTSAC 1 cut(s) 97
NsiI ATGCAT 1 cut(s) 770
OliI CACNNNNGTG 1 cut(s) 1003
PciSI GCTCTTC 1 cut(s) 513
PctI GAATGC 1 cut(s) 374
PfeI GAWTC 3 cut(s) 493, 635, 977
PflMI CCANNNNNTGG 1 cut(s) 860
PkrI GCNGC 4 cut(s) 253, 276, 544, 882
PleI GAGTC 3 cut(s) 334, 523, 874
PpsI GAGTC 3 cut(s) 334, 523, 874
PspN4I GGNNCC 3 cut(s) 144, 411, 852
PspPI GGNCC 1 cut(s) 626
PsrI GAACNNNNNNTAC 2 cut(s) 384, 416
PstNI CAGNNNCTG 2 cut(s) 254, 733
PsuI RGATCY 4 cut(s) 27, 188, 243, 264
PvuII CAGCTG 1 cut(s) 254
RsaI GTAC 4 cut(s) 47, 402, 844, 936
RsaNI GTAC 4 cut(s) 46, 401, 843, 935
RseI CAYNNNNRTG 1 cut(s) 1003
SapI GCTCTTC 1 cut(s) 513
SaqAI TTAA 3 cut(s) 473, 563, 761
SatI GCNGC 4 cut(s) 252, 275, 543, 881
Sau3AI GATC 5 cut(s) 27, 188, 243, 264, 615
Sau96I GGNCC 1 cut(s) 626
SchI GAGTC 3 cut(s) 335, 523, 875
ScrFI CCNGG 1 cut(s) 625
SfaNI GCATC 3 cut(s) 587, 745, 907
SfcI CTRYAG 1 cut(s) 537
SmiMI CAYNNNNRTG 1 cut(s) 1003
SpeI ACTAGT 1 cut(s) 161
SsiI CCGC 1 cut(s) 605
SspI AATATT 3 cut(s) 477, 759, 859
SspMI CTAG 1 cut(s) 162
StyD4I CCNGG 1 cut(s) 623
StyI CCWWGG 2 cut(s) 239, 345
TaaI ACNGT 3 cut(s) 127, 154, 685
TatI WGTACW 1 cut(s) 45
TfiI GAWTC 3 cut(s) 493, 635, 977
Tru1I TTAA 3 cut(s) 473, 563, 761
Tru9I TTAA 3 cut(s) 473, 563, 761
TscAI CASTG 2 cut(s) 106, 369
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 4 cut(s) 251, 274, 542, 880
Tsp45I GTSAC 1 cut(s) 97
TspDTI ATGAA 6 cut(s) 17, 457, 686, 708, 894, 969
TspGWI ACGGA 2 cut(s) 78, 824
TspRI CASTG 2 cut(s) 106, 369
Van91I CCANNNNNTGG 1 cut(s) 860
XagI CCTNNNNNAGG 1 cut(s) 327
XapI RAATTY 5 cut(s) 505, 565, 660, 830, 901
XspI CTAG 1 cut(s) 162
Zsp2I ATGCAT 1 cut(s) 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.