MD04G1203600.v1.1

Sucrose nonfermenting 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001327
Physical Location & Seq
Forward (+)
28972162 .. 28978044
5883 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1203600.v1.1.491

Sequence Viewer

Length: 1452 bp
ATGTTAGGGTCTGGTTCGGGTGCTGCACCTGCACATCAAAGCAGTAGACTTTCCGTACCAGTTTTAATTCCGTATCGGTTTGTCTGGCCTTATGGAGGAAGGAGTGTGTTTCTCAGTGGTTCTTTCACAAGGTGGTTAGAACCTATACCTATGGCTCCAATGGAGGGGTGTCCAACTGTATTTCAAGTTGTTTGGAATTTGACGCCTGGATATCATCAGTACAAATTTTTTGTTGATGGCCAATGGCGACATAATGAGCATCAGCCTTTTGTTAATGCGAATGGTGGAACAGTGAATACCATCTTTCTAGCTGGAGAAGATATGGTTCCTACGAGTTTTAGTCCTGAGACTTCTGGTGGGTCCAACATGGACGTAGATATGATTCCTACAAATGTAGAAACAGTTCTTAGGTTCTCACAGGCTGATCTCGATATCTCTCGACATCGTATATCAATGTTCTTGTCCAGACATACGGCTTATGAGTTGCTTCCAGAGTCAGGCAAGGTCATTGCCTTGGATAATAGATTACCTGTGAAGCAAGCATTCCATATCCTACATGAACAGGGAGTACCTGTGGCTCCTCTGTGGGATTTTGAGAAGGGCCAGTTTGTTGGAGTCCTTAGTGCGATGGACTTCATCCTAATCTTGAGAGAGCTTGGGAATCATGGATCCAACTTGACCGAGGAAGAGCTTGAGACTCATACTGTAGCAGCATGGAAAGAGGGAAAAATAAATCTTAACAGACAACTTGATGGCAATGGGAGATACTATCCACCCCATCTTGTCTCTGCCGGCCCTTACGATTCTCTCAAAGAAGTAGCTTTGAAAATTTTGCAAAACAAGGTGGCTACAGTTCCAATTGTCCATTCAACTTCGCAGGATGGTTTATTTCCACAACTATTGCACCTTGCTTCCCTCTGCGGAATACTAAAATGCATATGCAGGCATTTTAGACATTCTTCTAGCTCCTTGCCCATACTTCAATGTCCTATATCTGAGTTTCGTTTAGGCACATGGGTTCCAAATATTGGAGAGTCAAATGGACGGCCATTGGCAATGTTAAGACCAAATTCATCTCTTGCTGATGTCTTCTCTCTGTTGATACAAGCTGACGTTAGTTCAATACCTATAGTGGATGGTAATGATTCATTGCTGGATATTTATTCACGAAGTGATATCACTGCTTTGGCCAGAGATAAAGCTTATACACAGATTCACCTTGATGAATTGAGCATCCATCAGGCGTTGCTACTGGGACAAGATGCGAATTCTCCTTACGGCTTCCTCAGCGGGCAGAGATGTCAGATGTGTTTGCGGTCAGATTCTTTGCATAAAGTGATGGAGCGCTTGGCTAATCCTGGGGTCAGGAGACTTGTGATCGTGGAGGCTGGCAGCAAGCGTGTAGAAGGGATTATTTCGTTGAGCGATGTATTCAGGTTCTTGCTAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000266 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006833 GO:0006950 GO:0006996 GO:0007005 GO:0007031 GO:0007154 GO:0008047 GO:0008150 GO:0008152 GO:0009267 GO:0009605 GO:0009856 GO:0009859 GO:0009875 GO:0009893 GO:0009987 GO:0009991 GO:0010562 GO:0010604 GO:0016043 GO:0016301 GO:0016310 GO:0016559 GO:0016740 GO:0016772 GO:0016773 GO:0019207 GO:0019209 GO:0019220 GO:0019222 GO:0019538 GO:0019887 GO:0022414 GO:0030234 GO:0030295 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0031667 GO:0031668 GO:0031669 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0033554 GO:0033674 GO:0036211 GO:0042044 GO:0042149 GO:0042325 GO:0042327 GO:0042594 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044706 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048285 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051716 GO:0060255 GO:0065007 GO:0065009 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0098772 GO:0140096 GO:1901564 GO:2000377
KEGG Pathways
Metabolic & Signaling
ko04068 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 map04068 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410
Pfam Domains
Protein Families
AMPK1_CBM CBS

Protein Analysis

484

Amino Acids

53.47

Weight (kDa)

6.31

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMPK1_CBM PF16561 23 - 101 3.9e-23 Glycogen recognition site of AMP-activated protein kinase
CBS PF00571 349 - 395 4.5e-06 CBS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 37
Acc36I ACCTGC 1 cut(s) 37
AccB7I CCANNNNNTGG 1 cut(s) 1028
AccI GTMKAC 1 cut(s) 46
AciI CCGC 3 cut(s) 921, 1290, 1315
AclWI GGATC 2 cut(s) 663, 676
AcoI YGGCCR 3 cut(s) 238, 1046, 1188
AcsI RAATTY 5 cut(s) 196, 224, 828, 1069, 1267
AcyI GRCGYC 1 cut(s) 203
AdeI CACNNNGTG 2 cut(s) 132, 1172
AfaI GTAC 3 cut(s) 57, 221, 570
AfeI AGCGCT 1 cut(s) 1346
AfiI CCNNNNNNNGG 4 cut(s) 95, 164, 497, 1028
AgsI TTSAA 5 cut(s) 185, 826, 870, 983, 1122
AjnI CCWGG 2 cut(s) 205, 1357
AjuI GAANNNNNNNTTGG 2 cut(s) 850, 882
AluBI AGCT 7 cut(s) 311, 655, 691, 821, 966, 1109, 1202
AluI AGCT 7 cut(s) 311, 655, 691, 821, 966, 1109, 1202
Alw26I GTCTC 4 cut(s) 341, 689, 790, 1363
AlwI GGATC 2 cut(s) 663, 676
Aor51HI AGCGCT 1 cut(s) 1346
AoxI GGCC 6 cut(s) 86, 238, 601, 793, 1046, 1188
ApeKI GCWGC 3 cut(s) 23, 710, 1392
ApoI RAATTY 5 cut(s) 196, 224, 828, 1069, 1267
Asp700I GAANNNNTTC 1 cut(s) 402
AspLEI GCGC 1 cut(s) 1347
AspS9I GGNCC 3 cut(s) 360, 601, 794
AsuHPI GGTGA 1 cut(s) 1208
AvaII GGWCC 1 cut(s) 360
BalI TGGCCA 2 cut(s) 240, 1190
BamHI GGATCC 1 cut(s) 668
BbsI GAAGAC 1 cut(s) 1081
BbvCI CCTCAGC 1 cut(s) 1286
BbvI GCAGC 3 cut(s) 10, 722, 1404
BccI CCATC 9 cut(s) 230, 308, 622, 746, 786, 875, 1130, 1245, 1333
BceAI ACGGC 3 cut(s) 489, 1061, 1294
BciT130I CCWGG 2 cut(s) 207, 1359
BcoDI GTCTC 4 cut(s) 341, 689, 790, 1363
BfaI CTAG 4 cut(s) 308, 963, 1445, 1450
BfmI CTRYAG 3 cut(s) 705, 849, 1128
BfoI RGCGCY 1 cut(s) 1348
BfuAI ACCTGC 1 cut(s) 37
BisI GCNGC 3 cut(s) 24, 711, 1393
BlsI GCNGC 3 cut(s) 25, 712, 1394
Bme1390I CCNGG 2 cut(s) 207, 1359
Bme18I GGWCC 1 cut(s) 360
BmgT120I GGNCC 3 cut(s) 360, 601, 794
BmiI GGNNCC 6 cut(s) 156, 327, 361, 579, 670, 1020
BmrFI CCNGG 2 cut(s) 207, 1359
BmrI ACTGGG 1 cut(s) 1262
BmsI GCATC 3 cut(s) 268, 1242, 1252
BmuI ACTGGG 1 cut(s) 1262
BpiI GAAGAC 1 cut(s) 1081
BpmI CTGGAG 1 cut(s) 333
Bpu10I CCTNAGC 1 cut(s) 1286
BpuEI CTTGAG 2 cut(s) 667, 713
BsaHI GRCGYC 1 cut(s) 203
BsaJI CCNNGG 3 cut(s) 513, 681, 1358
BsaXI ACNNNNNCTCC 2 cut(s) 558, 588
Bsc4I CCNNNNNNNGG 4 cut(s) 95, 164, 497, 1028
Bse118I RCCGGY 1 cut(s) 791
Bse1I ACTGG 3 cut(s) 59, 604, 1257
Bse3DI GCAATG 4 cut(s) 507, 763, 1062, 1148
BseBI CCWGG 2 cut(s) 207, 1359
BseDI CCNNGG 3 cut(s) 513, 681, 1358
BseGI GGATG 4 cut(s) 636, 886, 1141, 1233
BseLI CCNNNNNNNGG 4 cut(s) 95, 164, 497, 1028
BseMI GCAATG 4 cut(s) 507, 763, 1062, 1148
BseMII CTCAG 4 cut(s) 127, 336, 987, 1300
BseNI ACTGG 3 cut(s) 59, 604, 1257
BseRI GAGGAG 1 cut(s) 570
BseXI GCAGC 3 cut(s) 10, 722, 1404
BsgI GTGCAG 2 cut(s) 9, 15
BshFI GGCC 6 cut(s) 88, 240, 603, 795, 1048, 1190
BsiSI CCGG 1 cut(s) 792
BslFI GGGAC 1 cut(s) 1269
BslI CCNNNNNNNGG 4 cut(s) 95, 164, 497, 1028
BsmAI GTCTC 4 cut(s) 341, 689, 790, 1363
BsmFI GGGAC 1 cut(s) 1269
BsmI GAATGC 1 cut(s) 542
BsnI GGCC 6 cut(s) 88, 240, 603, 795, 1048, 1190
Bsp143I GATC 3 cut(s) 424, 668, 1377
BspACI CCGC 3 cut(s) 921, 1290, 1315
BspANI GGCC 6 cut(s) 88, 240, 603, 795, 1048, 1190
BspCNI CTCAG 4 cut(s) 126, 337, 988, 1299
BspLI GGNNCC 6 cut(s) 156, 327, 361, 579, 670, 1020
BspMI ACCTGC 1 cut(s) 37
BspPI GGATC 2 cut(s) 663, 676
BspQI GCTCTTC 1 cut(s) 681
BsrDI GCAATG 4 cut(s) 507, 763, 1062, 1148
BsrFI RCCGGY 1 cut(s) 791
BsrI ACTGG 3 cut(s) 59, 604, 1257
BssAI RCCGGY 1 cut(s) 791
BssECI CCNNGG 3 cut(s) 513, 681, 1358
BssMI GATC 3 cut(s) 424, 668, 1377
BssNI GRCGYC 1 cut(s) 203
BssT1I CCWWGG 1 cut(s) 513
Bst2UI CCWGG 2 cut(s) 207, 1359
Bst4CI ACNGT 5 cut(s) 178, 292, 403, 706, 853
Bst6I CTCTTC 1 cut(s) 681
BstACI GRCGYC 1 cut(s) 203
BstC8I GCNNGC 6 cut(s) 540, 793, 944, 1292, 1390, 1397
BstDEI CTNAG 6 cut(s) 113, 345, 407, 620, 996, 1286
BstENI CCTNNNNNAGG 1 cut(s) 93
BstF5I GGATG 4 cut(s) 636, 886, 1141, 1233
BstH2I RGCGCY 1 cut(s) 1348
BstHHI GCGC 1 cut(s) 1347
BstKTI GATC 3 cut(s) 427, 671, 1380
BstMAI GTCTC 4 cut(s) 341, 689, 790, 1363
BstMBI GATC 3 cut(s) 424, 668, 1377
BstMWI GCNNNNNNNGC 2 cut(s) 29, 1287
BstNI CCWGG 2 cut(s) 207, 1359
BstSCI CCNGG 2 cut(s) 205, 1357
BstSFI CTRYAG 3 cut(s) 705, 849, 1128
BstV1I GCAGC 3 cut(s) 10, 722, 1404
BstV2I GAAGAC 1 cut(s) 1081
BstX2I RGATCY 1 cut(s) 668
BstXI CCANNNNNNTGG 1 cut(s) 611
BstYI RGATCY 1 cut(s) 668
BsuRI GGCC 6 cut(s) 88, 240, 603, 795, 1048, 1190
BtgZI GCGATG 2 cut(s) 641, 1440
BtsCI GGATG 4 cut(s) 636, 886, 1141, 1233
BtsI GCAGTG 1 cut(s) 1179
BtsIMutI CAGTG 3 cut(s) 121, 297, 1179
BveI ACCTGC 1 cut(s) 37
Cac8I GCNNGC 6 cut(s) 540, 793, 944, 1292, 1390, 1397
CfoI GCGC 1 cut(s) 1347
Cfr10I RCCGGY 1 cut(s) 791
Cfr13I GGNCC 3 cut(s) 360, 601, 794
CseI GACGC 1 cut(s) 211
Csp6I GTAC 3 cut(s) 56, 220, 569
CspCI CAANNNNNGTGG 2 cut(s) 882, 917
CviAII CATG 5 cut(s) 367, 557, 665, 714, 1014
CviQI GTAC 3 cut(s) 56, 220, 569
DdeI CTNAG 6 cut(s) 113, 345, 407, 620, 996, 1286
DpnI GATC 3 cut(s) 426, 670, 1379
DpnII GATC 3 cut(s) 424, 668, 1377
DraIII CACNNNGTG 2 cut(s) 132, 1172
EaeI YGGCCR 3 cut(s) 238, 1046, 1188
Eam1104I CTCTTC 1 cut(s) 681
EarI CTCTTC 1 cut(s) 681
Eco130I CCWWGG 1 cut(s) 513
Eco32I GATATC 3 cut(s) 212, 433, 1177
Eco47I GGWCC 1 cut(s) 360
Eco47III AGCGCT 1 cut(s) 1346
EcoNI CCTNNNNNAGG 1 cut(s) 93
EcoRI GAATTC 1 cut(s) 1267
EcoRII CCWGG 2 cut(s) 205, 1357
EcoRV GATATC 3 cut(s) 212, 433, 1177
EcoT14I CCWWGG 1 cut(s) 513
EcoT22I ATGCAT 1 cut(s) 938
ErhI CCWWGG 1 cut(s) 513
FaeI CATG 5 cut(s) 370, 560, 668, 717, 1017
FaqI GGGAC 1 cut(s) 1269
FatI CATG 5 cut(s) 366, 556, 664, 713, 1013
FauI CCCGC 1 cut(s) 1283
FauNDI CATATG 1 cut(s) 938
FblI GTMKAC 1 cut(s) 46
Fnu4HI GCNGC 3 cut(s) 24, 711, 1393
FokI GGATG 4 cut(s) 623, 893, 1148, 1220
Fsp4HI GCNGC 3 cut(s) 24, 711, 1393
FspBI CTAG 4 cut(s) 308, 963, 1445, 1450
GlaI GCGC 1 cut(s) 1346
GluI GCNGC 3 cut(s) 24, 711, 1393
GsuI CTGGAG 1 cut(s) 333
HaeII RGCGCY 1 cut(s) 1348
HaeIII GGCC 6 cut(s) 88, 240, 603, 795, 1048, 1190
HapII CCGG 1 cut(s) 792
HgaI GACGC 1 cut(s) 211
HhaI GCGC 1 cut(s) 1347
Hin1I GRCGYC 1 cut(s) 203
Hin1II CATG 5 cut(s) 370, 560, 668, 717, 1017
Hin6I GCGC 1 cut(s) 1345
HinP1I GCGC 1 cut(s) 1345
HindIII AAGCTT 1 cut(s) 1200
HpaII CCGG 1 cut(s) 792
HphI GGTGA 1 cut(s) 1208
Hpy166II GTNNAC 1 cut(s) 47
Hpy188I TCNGA 3 cut(s) 997, 1305, 1321
Hpy188III TCNNGA 8 cut(s) 344, 428, 438, 465, 491, 646, 1167, 1366
Hpy8I GTNNAC 1 cut(s) 47
HpyAV CCTTC 3 cut(s) 93, 592, 1400
HpyCH4III ACNGT 5 cut(s) 178, 292, 403, 706, 853
HpyCH4IV ACGT 2 cut(s) 372, 1113
HpyCH4V TGCA 7 cut(s) 26, 32, 835, 904, 936, 942, 1330
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 1287
HpyF3I CTNAG 6 cut(s) 113, 345, 407, 620, 996, 1286
HpySE526I ACGT 2 cut(s) 372, 1113
Hsp92I GRCGYC 1 cut(s) 203
Hsp92II CATG 5 cut(s) 370, 560, 668, 717, 1017
HspAI GCGC 1 cut(s) 1345
KroI GCCGGC 1 cut(s) 791
KroNI GCCGGC 1 cut(s) 793
Kzo9I GATC 3 cut(s) 424, 668, 1377
LguI GCTCTTC 1 cut(s) 681
LmnI GCTCC 4 cut(s) 160, 583, 971, 1342
Lsp1109I GCAGC 3 cut(s) 10, 722, 1404
LweI GCATC 3 cut(s) 268, 1242, 1252
MaeI CTAG 4 cut(s) 308, 963, 1445, 1450
MaeII ACGT 2 cut(s) 372, 1113
MalI GATC 3 cut(s) 426, 670, 1379
MboI GATC 3 cut(s) 424, 668, 1377
MboII GAAGA 4 cut(s) 329, 698, 951, 1081
MfeI CAATTG 1 cut(s) 858
MflI RGATCY 1 cut(s) 668
MlsI TGGCCA 2 cut(s) 240, 1190
MluCI AATT 8 cut(s) 66, 196, 224, 828, 858, 1069, 1226, 1267
MluNI TGGCCA 2 cut(s) 240, 1190
MlyI GAGTC 4 cut(s) 503, 624, 691, 1043
MmeI TCCRAC 4 cut(s) 197, 387, 592, 696
MnlI CCTC 8 cut(s) 89, 157, 591, 676, 715, 926, 1295, 1378
Mox20I TGGCCA 2 cut(s) 240, 1190
Mph1103I ATGCAT 1 cut(s) 938
MroNI GCCGGC 1 cut(s) 791
MroXI GAANNNNTTC 1 cut(s) 402
MscI TGGCCA 2 cut(s) 240, 1190
MseI TTAA 4 cut(s) 65, 273, 738, 1061
MslI CAYNNNNRTG 1 cut(s) 1221
Msp20I TGGCCA 2 cut(s) 240, 1190
MspA1I CMGCKG 1 cut(s) 1290
MspI CCGG 1 cut(s) 792
MspR9I CCNGG 2 cut(s) 207, 1359
MunI CAATTG 1 cut(s) 858
Mva1269I GAATGC 1 cut(s) 542
MvaI CCWGG 2 cut(s) 207, 1359
MwoI GCNNNNNNNGC 2 cut(s) 29, 1287
NaeI GCCGGC 1 cut(s) 793
NdeI CATATG 1 cut(s) 938
NdeII GATC 3 cut(s) 424, 668, 1377
NgoMIV GCCGGC 1 cut(s) 791
NlaIII CATG 5 cut(s) 370, 560, 668, 717, 1017
NlaIV GGNNCC 6 cut(s) 156, 327, 361, 579, 670, 1020
NsiI ATGCAT 1 cut(s) 938
PaqCI CACCTGC 1 cut(s) 37
PciSI GCTCTTC 1 cut(s) 681
PctI GAATGC 1 cut(s) 542
PdiI GCCGGC 1 cut(s) 793
PdmI GAANNNNTTC 1 cut(s) 402
PfeI GAWTC 6 cut(s) 382, 661, 803, 1145, 1213, 1322
PflMI CCANNNNNTGG 1 cut(s) 1028
PkrI GCNGC 3 cut(s) 25, 712, 1394
PleI GAGTC 4 cut(s) 502, 623, 691, 1042
PpsI GAGTC 4 cut(s) 502, 623, 691, 1042
Psp6I CCWGG 2 cut(s) 205, 1357
PspGI CCWGG 2 cut(s) 205, 1357
PspN4I GGNNCC 6 cut(s) 156, 327, 361, 579, 670, 1020
PspPI GGNCC 3 cut(s) 360, 601, 794
PsrI GAACNNNNNNTAC 6 cut(s) 280, 312, 387, 419, 552, 584
PsuI RGATCY 1 cut(s) 668
RsaI GTAC 3 cut(s) 57, 221, 570
RsaNI GTAC 3 cut(s) 56, 220, 569
RseI CAYNNNNRTG 1 cut(s) 1221
SapI GCTCTTC 1 cut(s) 681
SaqAI TTAA 4 cut(s) 65, 273, 738, 1061
SatI GCNGC 3 cut(s) 24, 711, 1393
Sau3AI GATC 3 cut(s) 424, 668, 1377
Sau96I GGNCC 3 cut(s) 360, 601, 794
SchI GAGTC 4 cut(s) 503, 624, 691, 1043
ScrFI CCNGG 2 cut(s) 207, 1359
SfaNI GCATC 3 cut(s) 268, 1242, 1252
SfcI CTRYAG 3 cut(s) 705, 849, 1128
SinI GGWCC 1 cut(s) 360
SmiMI CAYNNNNRTG 1 cut(s) 1221
SmlI CTYRAG 2 cut(s) 646, 692
SmoI CTYRAG 2 cut(s) 646, 692
Sse9I AATT 8 cut(s) 66, 196, 224, 828, 858, 1069, 1226, 1267
SsiI CCGC 3 cut(s) 921, 1290, 1315
SspI AATATT 1 cut(s) 1027
SspMI CTAG 4 cut(s) 308, 963, 1445, 1450
StyD4I CCNGG 2 cut(s) 205, 1357
StyI CCWWGG 1 cut(s) 513
TaaI ACNGT 5 cut(s) 178, 292, 403, 706, 853
TaiI ACGT 2 cut(s) 375, 1116
TaqI TCGA 2 cut(s) 429, 439
TaqII GACCGA 1 cut(s) 695
TasI AATT 8 cut(s) 66, 196, 224, 828, 858, 1069, 1226, 1267
TatI WGTACW 1 cut(s) 219
TfiI GAWTC 6 cut(s) 382, 661, 803, 1145, 1213, 1322
Tru1I TTAA 4 cut(s) 65, 273, 738, 1061
Tru9I TTAA 4 cut(s) 65, 273, 738, 1061
TscAI CASTG 3 cut(s) 121, 297, 1186
TseI GCWGC 3 cut(s) 23, 710, 1392
TspDTI ATGAA 5 cut(s) 573, 625, 1062, 1137, 1239
TspGWI ACGGA 2 cut(s) 43, 60
TspRI CASTG 3 cut(s) 121, 297, 1186
Van91I CCANNNNNTGG 1 cut(s) 1028
VpaK11BI GGWCC 1 cut(s) 360
XagI CCTNNNNNAGG 1 cut(s) 93
XapI RAATTY 5 cut(s) 196, 224, 828, 1069, 1267
XmiI GTMKAC 1 cut(s) 46
XmnI GAANNNNTTC 1 cut(s) 402
XspI CTAG 4 cut(s) 308, 963, 1445, 1450
Zsp2I ATGCAT 1 cut(s) 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.