Rh3BG067300

Sucrose nonfermenting 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001327
Physical Location & Seq
Reverse (-)
4538902 .. 4545183
6282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG067300.1

Sequence Viewer

Length: 1029 bp
ATGCTGTTAGTTGGTATCTTGCAGGAAGTAGCTCCAAGGATCTCAGCAGCTGAATTGGAGATCTCTCAGCAGCGTATATCAGTATTTCTATCCACACATACTGCTTATGAGTTGCTTCCTGAGTCAGGCAAGGTCATTGCCTTGGATGTCAATTTACCAGTGAAAGAAGCATTCCATATCCTACACGAACAGGGAGTACCTGTGGCTCCTCTCTGGGATTTTGAAAAGGGACAATTTGTAGGAGTTCTTAGCGCATTGGATTTCATCTTAATATTGAGAGAGCTTGGGAATCATGGGTCAAATTTGACTGGGGAAGAGCTGGAGACTCATACTATAGCAGCATGGAAAGAGGGAAAATTAAATTTGAACAGACAACTTGATGGAAATGGGAGATGCTATCCGCCACATTTGATCTCTGCCGGGCCTTATGATTCTCTCAAAGAAGTTGCTGTCAGAATTCTGCAAAACAAGGTGGCCACAGTTCCAATTCTTCATACTTCCTCAGAGGATGGTTCATTTCCACAGCTACTGCATCTTGCCTCCCTTTCGGGAATATTAAAATGCATATGCAGGCATTTCAGACATTCTTCTACCTCCTTGCCAATACTCCAACATCCTATTTCAGAATTCCGTATAGGTACATGGGTTCCAAATATTGGGGAGTCAAATGGAAGGCTGCTGGCCATGTTGAGACCAAATTCATCTCTTGCTGATGCCCTGTCTTTGTTGGTACAAGCTGAAGTTAGTTCAATACCCATAGTGGATGACAATGATTCATTGCTGGATGTTTATTCACGAAGTGATATTACTGCTTTGGCAAGAGACAAAGCTTATGCACAGATTCGCCTTGATCAATTGACTATCCATCAGACCTTGCAACTTGGGCAAAATGCAAATTCTCCTTATGGCTTCTTCAGTGGTCAGAGATGTCAGATGTGTTTGCGATCTGATCCTCTGCATAAAGTGATGGAGCGGTTGGCGAATCCAGGTCTCATCGGCTTTTGCTATTTTAAGACCCCTATCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000266 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006833 GO:0006950 GO:0006996 GO:0007005 GO:0007031 GO:0007154 GO:0008047 GO:0008150 GO:0008152 GO:0009267 GO:0009605 GO:0009856 GO:0009859 GO:0009875 GO:0009893 GO:0009987 GO:0009991 GO:0010562 GO:0010604 GO:0016043 GO:0016301 GO:0016310 GO:0016559 GO:0016740 GO:0016772 GO:0016773 GO:0019207 GO:0019209 GO:0019220 GO:0019222 GO:0019538 GO:0019887 GO:0022414 GO:0030234 GO:0030295 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0031667 GO:0031668 GO:0031669 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0033554 GO:0033674 GO:0036211 GO:0042044 GO:0042149 GO:0042325 GO:0042327 GO:0042594 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044706 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048285 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051716 GO:0060255 GO:0065007 GO:0065009 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0098772 GO:0140096 GO:1901564 GO:2000377
KEGG Pathways
Metabolic & Signaling
ko04068 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 map04068 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410
Pfam Domains
Protein Families
AMPK1_CBM CBS

Protein Analysis

342

Amino Acids

37.78

Weight (kDa)

6.1

Isoelectric Point (pI)

40.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBS PF00571 229 - 271 2.9e-06 CBS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 656
AccBSI CCGCTC 1 cut(s) 973
AciI CCGC 2 cut(s) 401, 973
AclWI GGATC 2 cut(s) 47, 944
AcoI YGGCCR 2 cut(s) 474, 681
AcsI RAATTY 6 cut(s) 301, 361, 456, 626, 697, 895
AcuI CTGAAG 2 cut(s) 759, 898
AdeI CACNNNGTG 1 cut(s) 800
AfaI GTAC 3 cut(s) 198, 640, 732
AfiI CCNNNNNNNGG 2 cut(s) 125, 656
AgsI TTSAA 3 cut(s) 224, 367, 750
AjnI CCWGG 1 cut(s) 985
AluBI AGCT 7 cut(s) 32, 50, 283, 319, 526, 737, 830
AluI AGCT 7 cut(s) 32, 50, 283, 319, 526, 737, 830
Alw26I GTCTC 4 cut(s) 317, 685, 816, 995
AlwI GGATC 2 cut(s) 47, 944
AlwNI CAGNNNCTG 2 cut(s) 50, 529
AoxI GGCC 3 cut(s) 422, 474, 681
ApeKI GCWGC 4 cut(s) 47, 70, 338, 676
ApoI RAATTY 6 cut(s) 301, 361, 456, 626, 697, 895
AspLEI GCGC 1 cut(s) 254
AspS9I GGNCC 1 cut(s) 422
AsuC2I CCSGG 1 cut(s) 421
BaeI ACNNNNGTAYC 2 cut(s) 630, 663
BalI TGGCCA 2 cut(s) 476, 683
BarI GAAGNNNNNNTAC 2 cut(s) 790, 822
BbvI GCAGC 4 cut(s) 59, 82, 350, 663
BccI CCATC 4 cut(s) 374, 503, 873, 961
BciT130I CCWGG 1 cut(s) 987
BclI TGATCA 1 cut(s) 850
BcnI CCSGG 1 cut(s) 421
BcoDI GTCTC 4 cut(s) 317, 685, 816, 995
BfmI CTRYAG 1 cut(s) 333
BglII AGATCT 1 cut(s) 60
BisI GCNGC 4 cut(s) 48, 71, 339, 677
BlsI GCNGC 4 cut(s) 49, 72, 340, 678
Bme1390I CCNGG 2 cut(s) 421, 987
BmgT120I GGNCC 1 cut(s) 422
BmiI GGNNCC 2 cut(s) 207, 648
BmrFI CCNGG 2 cut(s) 421, 987
BmrI ACTGGG 1 cut(s) 318
BmsI GCATC 3 cut(s) 383, 541, 703
BmuI ACTGGG 1 cut(s) 318
BpmI CTGGAG 1 cut(s) 341
BpuMI CCSGG 1 cut(s) 421
BsaI GGTCTC 2 cut(s) 685, 995
BsaJI CCNNGG 2 cut(s) 35, 141
BsaXI ACNNNNNCTCC 2 cut(s) 186, 216
Bsc4I CCNNNNNNNGG 2 cut(s) 125, 656
Bse1I ACTGG 2 cut(s) 158, 313
Bse3DI GCAATG 2 cut(s) 135, 776
BseBI CCWGG 1 cut(s) 987
BseDI CCNNGG 2 cut(s) 35, 141
BseGI GGATG 5 cut(s) 151, 514, 613, 769, 790
BseLI CCNNNNNNNGG 2 cut(s) 125, 656
BseMI GCAATG 2 cut(s) 135, 776
BseMII CTCAG 4 cut(s) 57, 80, 111, 516
BseNI ACTGG 2 cut(s) 158, 313
BseRI GAGGAG 1 cut(s) 198
BseXI GCAGC 4 cut(s) 59, 82, 350, 663
BshFI GGCC 3 cut(s) 424, 476, 683
BsiSI CCGG 1 cut(s) 420
BslFI GGGAC 1 cut(s) 243
BslI CCNNNNNNNGG 2 cut(s) 125, 656
BsmAI GTCTC 4 cut(s) 317, 685, 816, 995
BsmFI GGGAC 1 cut(s) 243
BsmI GAATGC 1 cut(s) 170
BsnI GGCC 3 cut(s) 424, 476, 683
Bso31I GGTCTC 2 cut(s) 685, 995
Bsp143I GATC 6 cut(s) 39, 60, 411, 850, 944, 949
BspACI CCGC 2 cut(s) 401, 973
BspANI GGCC 3 cut(s) 424, 476, 683
BspCNI CTCAG 4 cut(s) 56, 79, 112, 515
BspLI GGNNCC 2 cut(s) 207, 648
BspPI GGATC 2 cut(s) 47, 944
BspQI GCTCTTC 1 cut(s) 309
BspTNI GGTCTC 2 cut(s) 685, 995
BsrBI CCGCTC 1 cut(s) 973
BsrDI GCAATG 2 cut(s) 135, 776
BsrI ACTGG 2 cut(s) 158, 313
BssECI CCNNGG 2 cut(s) 35, 141
BssMI GATC 6 cut(s) 39, 60, 411, 850, 944, 949
BssT1I CCWWGG 2 cut(s) 35, 141
Bst2UI CCWGG 1 cut(s) 987
Bst4CI ACNGT 1 cut(s) 481
Bst6I CTCTTC 1 cut(s) 309
BstC8I GCNNGC 2 cut(s) 572, 681
BstDEI CTNAG 5 cut(s) 43, 66, 120, 248, 502
BstENI CCTNNNNNAGG 1 cut(s) 123
BstF5I GGATG 5 cut(s) 151, 514, 613, 769, 790
BstHHI GCGC 1 cut(s) 254
BstKTI GATC 6 cut(s) 42, 63, 414, 853, 947, 952
BstMAI GTCTC 4 cut(s) 317, 685, 816, 995
BstMBI GATC 6 cut(s) 39, 60, 411, 850, 944, 949
BstMWI GCNNNNNNNGC 1 cut(s) 883
BstNI CCWGG 1 cut(s) 987
BstSCI CCNGG 2 cut(s) 419, 985
BstSFI CTRYAG 1 cut(s) 333
BstV1I GCAGC 4 cut(s) 59, 82, 350, 663
BstX2I RGATCY 2 cut(s) 39, 60
BstYI RGATCY 2 cut(s) 39, 60
BsuRI GGCC 3 cut(s) 424, 476, 683
BtsCI GGATG 5 cut(s) 151, 514, 613, 769, 790
BtsIMutI CAGTG 2 cut(s) 165, 922
Cac8I GCNNGC 2 cut(s) 572, 681
CaiI CAGNNNCTG 2 cut(s) 50, 529
CfoI GCGC 1 cut(s) 254
Cfr13I GGNCC 1 cut(s) 422
Csp6I GTAC 3 cut(s) 197, 639, 731
CviAII CATG 4 cut(s) 293, 342, 642, 685
CviQI GTAC 3 cut(s) 197, 639, 731
DdeI CTNAG 5 cut(s) 43, 66, 120, 248, 502
DpnI GATC 6 cut(s) 41, 62, 413, 852, 946, 951
DpnII GATC 6 cut(s) 39, 60, 411, 850, 944, 949
DraIII CACNNNGTG 1 cut(s) 800
EaeI YGGCCR 2 cut(s) 474, 681
Eam1104I CTCTTC 1 cut(s) 309
EarI CTCTTC 1 cut(s) 309
EciI GGCGGA 1 cut(s) 390
Eco130I CCWWGG 2 cut(s) 35, 141
Eco31I GGTCTC 2 cut(s) 685, 995
Eco57I CTGAAG 2 cut(s) 759, 898
EcoNI CCTNNNNNAGG 1 cut(s) 123
EcoRI GAATTC 2 cut(s) 456, 626
EcoRII CCWGG 1 cut(s) 985
EcoT14I CCWWGG 2 cut(s) 35, 141
EcoT22I ATGCAT 1 cut(s) 566
ErhI CCWWGG 2 cut(s) 35, 141
FaeI CATG 4 cut(s) 296, 345, 645, 688
FaqI GGGAC 1 cut(s) 243
FatI CATG 4 cut(s) 292, 341, 641, 684
FauNDI CATATG 1 cut(s) 566
FbaI TGATCA 1 cut(s) 850
Fnu4HI GCNGC 4 cut(s) 48, 71, 339, 677
FokI GGATG 5 cut(s) 158, 521, 600, 776, 797
Fsp4HI GCNGC 4 cut(s) 48, 71, 339, 677
GlaI GCGC 1 cut(s) 253
GluI GCNGC 4 cut(s) 48, 71, 339, 677
GsuI CTGGAG 1 cut(s) 341
HaeIII GGCC 3 cut(s) 424, 476, 683
HapII CCGG 1 cut(s) 420
HhaI GCGC 1 cut(s) 254
Hin1II CATG 4 cut(s) 296, 345, 645, 688
Hin6I GCGC 1 cut(s) 252
HinP1I GCGC 1 cut(s) 252
HindIII AAGCTT 1 cut(s) 828
HinfI GANTC 8 cut(s) 122, 289, 325, 431, 662, 773, 841, 982
HpaII CCGG 1 cut(s) 420
Hpy188I TCNGA 8 cut(s) 455, 505, 581, 625, 870, 924, 933, 949
Hpy188III TCNNGA 3 cut(s) 119, 549, 795
HpyAV CCTTC 1 cut(s) 666
HpyCH4III ACNGT 1 cut(s) 481
HpyCH4V TGCA 9 cut(s) 22, 463, 532, 564, 570, 836, 877, 893, 958
HpyF10VI GCNNNNNNNGC 1 cut(s) 883
HpyF3I CTNAG 5 cut(s) 43, 66, 120, 248, 502
Hsp92II CATG 4 cut(s) 296, 345, 645, 688
HspAI GCGC 1 cut(s) 252
Ksp22I TGATCA 1 cut(s) 850
Kzo9I GATC 6 cut(s) 39, 60, 411, 850, 944, 949
LguI GCTCTTC 1 cut(s) 309
LmnI GCTCC 3 cut(s) 37, 211, 970
Lsp1109I GCAGC 4 cut(s) 59, 82, 350, 663
LweI GCATC 3 cut(s) 383, 541, 703
MalI GATC 6 cut(s) 41, 62, 413, 852, 946, 951
MbiI CCGCTC 1 cut(s) 973
MboI GATC 6 cut(s) 39, 60, 411, 850, 944, 949
MboII GAAGA 4 cut(s) 326, 482, 579, 904
MfeI CAATTG 1 cut(s) 854
MflI RGATCY 2 cut(s) 39, 60
MlsI TGGCCA 2 cut(s) 476, 683
MluNI TGGCCA 2 cut(s) 476, 683
MlyI GAGTC 3 cut(s) 131, 319, 671
MmeI TCCRAC 1 cut(s) 634
MnlI CCTC 7 cut(s) 219, 343, 499, 511, 550, 604, 963
Mox20I TGGCCA 2 cut(s) 476, 683
Mph1103I ATGCAT 1 cut(s) 566
MscI TGGCCA 2 cut(s) 476, 683
MseI TTAA 4 cut(s) 269, 359, 557, 1011
Msp20I TGGCCA 2 cut(s) 476, 683
MspA1I CMGCKG 1 cut(s) 50
MspI CCGG 1 cut(s) 420
MspR9I CCNGG 2 cut(s) 421, 987
MunI CAATTG 1 cut(s) 854
Mva1269I GAATGC 1 cut(s) 170
MvaI CCWGG 1 cut(s) 987
MwoI GCNNNNNNNGC 1 cut(s) 883
NciI CCSGG 1 cut(s) 421
NdeI CATATG 1 cut(s) 566
NdeII GATC 6 cut(s) 39, 60, 411, 850, 944, 949
NlaIII CATG 4 cut(s) 296, 345, 645, 688
NlaIV GGNNCC 2 cut(s) 207, 648
NsiI ATGCAT 1 cut(s) 566
PciSI GCTCTTC 1 cut(s) 309
PctI GAATGC 1 cut(s) 170
PfeI GAWTC 5 cut(s) 289, 431, 773, 841, 982
PflMI CCANNNNNTGG 1 cut(s) 656
PkrI GCNGC 4 cut(s) 49, 72, 340, 678
PleI GAGTC 3 cut(s) 130, 319, 670
PpsI GAGTC 3 cut(s) 130, 319, 670
Psp6I CCWGG 1 cut(s) 985
PspGI CCWGG 1 cut(s) 985
PspN4I GGNNCC 2 cut(s) 207, 648
PspPI GGNCC 1 cut(s) 422
PsrI GAACNNNNNNTAC 2 cut(s) 180, 212
PstNI CAGNNNCTG 2 cut(s) 50, 529
PsuI RGATCY 2 cut(s) 39, 60
PvuII CAGCTG 1 cut(s) 50
RsaI GTAC 3 cut(s) 198, 640, 732
RsaNI GTAC 3 cut(s) 197, 639, 731
SapI GCTCTTC 1 cut(s) 309
SaqAI TTAA 4 cut(s) 269, 359, 557, 1011
SatI GCNGC 4 cut(s) 48, 71, 339, 677
Sau3AI GATC 6 cut(s) 39, 60, 411, 850, 944, 949
Sau96I GGNCC 1 cut(s) 422
SchI GAGTC 3 cut(s) 131, 319, 671
ScrFI CCNGG 2 cut(s) 421, 987
SfaNI GCATC 3 cut(s) 383, 541, 703
SfcI CTRYAG 1 cut(s) 333
SsiI CCGC 2 cut(s) 401, 973
SspI AATATT 3 cut(s) 273, 555, 655
StyD4I CCNGG 2 cut(s) 419, 985
StyI CCWWGG 2 cut(s) 35, 141
TaaI ACNGT 1 cut(s) 481
TfiI GAWTC 5 cut(s) 289, 431, 773, 841, 982
Tru1I TTAA 4 cut(s) 269, 359, 557, 1011
Tru9I TTAA 4 cut(s) 269, 359, 557, 1011
TscAI CASTG 2 cut(s) 165, 922
TseI GCWGC 4 cut(s) 47, 70, 338, 676
TspDTI ATGAA 5 cut(s) 253, 482, 504, 690, 765
TspGWI ACGGA 1 cut(s) 620
TspRI CASTG 2 cut(s) 165, 922
Van91I CCANNNNNTGG 1 cut(s) 656
XagI CCTNNNNNAGG 1 cut(s) 123
XapI RAATTY 6 cut(s) 301, 361, 456, 626, 697, 895
Zsp2I ATGCAT 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.