Rh3DG067300

Sucrose nonfermenting 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001327
Physical Location & Seq
Reverse (-)
4540262 .. 4546095
5834 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG067300.1

Sequence Viewer

Length: 1296 bp
ATGTTTGGTTCTGGTCACGGTACGGCATACCAGAGCAGTGGACTTCCAGGACCCATTTTAATTCCGACCCGCTTCGTCTGGCCTTATGGGGGAAGAAGGGTGTTCCTCAGTGGCTCTTTTACAAGGTGGTTAGAACATATACCTATGTCTCCAATGGAGGGGTGCCCAACTGCGTTTCAAGTTGTTTGGAACTTAACGCCTGGATATCACCAGTACAAGTTCTTGGTTGACGGAGAATGGCGACATAACGAGCAACTGCCTTTTGTCACTGGGAATTGTGGAATAGTGAATACCGTCTTTCTAACTGGGGAGCCAGATACAGTTCCTACTAGTTTCAGTCCTGAGACTTCTGGCAGATCCAATATGGATGTAGATAACGAAGTCTTTACGCGTATGGAAGCAGCTCCAAGGATCTCAGCGGCTGAATTGGAGGTCTCTCGGCAGCGTATATCAGTATTTCTATCCACACATACTGCTTATGAGTTGCTTCCTGAGTCAGGCAAGGTCATTGCCTTGGATGTCAATTTACCAGTGAAAGAAGCATTCCATATCCTACACGAACAGGGAGTACCTGTGGCTCCTCTCTGGGATTTTGAAAAGGGACAATTTGTAGGAGTTCTTAGCGCATTGGATTTCATCTTAATATTGAGAGAGCTTGGGAATCATGGGTCAAATTTGACCGGGGAAGAGCTGGAGACTCATACTATAGCAGCATGGAAAGAGGGAAAATTAAATTTGAACAGACAACTTGATGGAAATGGGAGATGCTATCCGCCACATTTGATCTCTGCTGGGCCTTATGATTCTCTCAAAGAAGTTGCTGTCAGAATTCTGCAAAACAAGGTGGCCACAGTTCCAATTCTTCATACTTCCTCAGAGGATGGTTCATTTCCACAGCTATTGCATCTTGCCTCCCTTTCGGGAATATTAAAATGCATATGCAGGCATTTCAGACATTCTTCTACCTCCTTGCCAATACTCCAACATCCTATTTCAGAATTCCGTATAGGTACATGGGTTCCAAATATTGGGGAGTCAAATGGAAGGCTGCTGGCTATGTTGAGACCAAATTCATCTCTTGCTGATGCCCTGTCTTTGTTGGTACAAGCTGAAGTTAGTTCAATACCCATAGTGGATGACAATGATTCATTGCTGGATGTTTATTCACGAAGTGATATTACTGCTTTGGCAAGAGACAAAGCTTATGCACAGATTCGCCTTGATCAATTGACTATCCATCAGGTAATTGCTAAATATAAAGGACTGATGAGGCTTTTACATGTGGGATTGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000266 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006833 GO:0006950 GO:0006996 GO:0007005 GO:0007031 GO:0007154 GO:0008047 GO:0008150 GO:0008152 GO:0009267 GO:0009605 GO:0009856 GO:0009859 GO:0009875 GO:0009893 GO:0009987 GO:0009991 GO:0010562 GO:0010604 GO:0016043 GO:0016301 GO:0016310 GO:0016559 GO:0016740 GO:0016772 GO:0016773 GO:0019207 GO:0019209 GO:0019220 GO:0019222 GO:0019538 GO:0019887 GO:0022414 GO:0030234 GO:0030295 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0031667 GO:0031668 GO:0031669 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0033554 GO:0033674 GO:0036211 GO:0042044 GO:0042149 GO:0042325 GO:0042327 GO:0042594 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044706 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048285 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051716 GO:0060255 GO:0065007 GO:0065009 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0098772 GO:0140096 GO:1901564 GO:2000377
KEGG Pathways
Metabolic & Signaling
ko04068 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 map04068 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410
Pfam Domains
Protein Families
AMPK1_CBM CBS

Protein Analysis

431

Amino Acids

47.76

Weight (kDa)

6.09

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMPK1_CBM PF16561 21 - 101 3.5e-25 Glycogen recognition site of AMP-activated protein kinase
CBS PF00571 353 - 395 4e-06 CBS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 162
AccB7I CCANNNNNTGG 1 cut(s) 1028
AccII CGCG 1 cut(s) 391
AciI CCGC 3 cut(s) 70, 419, 773
AclWI GGATC 2 cut(s) 351, 419
AcoI YGGCCR 1 cut(s) 846
AcsI RAATTY 5 cut(s) 673, 733, 828, 998, 1069
AcuI CTGAAG 1 cut(s) 1131
AdeI CACNNNGTG 1 cut(s) 1172
AfaI GTAC 5 cut(s) 22, 215, 570, 1012, 1104
AfiI CCNNNNNNNGG 4 cut(s) 89, 158, 497, 1028
AflIII ACRYGT 2 cut(s) 389, 1279
AgsI TTSAA 4 cut(s) 179, 596, 739, 1122
AhlI ACTAGT 1 cut(s) 329
AjnI CCWGG 2 cut(s) 46, 199
AluBI AGCT 6 cut(s) 404, 655, 691, 898, 1109, 1202
AluI AGCT 6 cut(s) 404, 655, 691, 898, 1109, 1202
Alw26I GTCTC 6 cut(s) 153, 338, 439, 689, 1057, 1188
AlwI GGATC 2 cut(s) 351, 419
AlwNI CAGNNNCTG 1 cut(s) 422
AoxI GGCC 3 cut(s) 80, 794, 846
ApeKI GCWGC 4 cut(s) 401, 442, 710, 1048
ApoI RAATTY 5 cut(s) 673, 733, 828, 998, 1069
AspLEI GCGC 1 cut(s) 626
AspS9I GGNCC 2 cut(s) 50, 794
AsuC2I CCSGG 1 cut(s) 682
AsuHPI GGTGA 1 cut(s) 200
AvaII GGWCC 1 cut(s) 50
BaeGI GKGCMC 1 cut(s) 167
BaeI ACNNNNGTAYC 2 cut(s) 1002, 1035
BalI TGGCCA 1 cut(s) 848
BanI GGYRCC 1 cut(s) 162
BarI GAAGNNNNNNTAC 2 cut(s) 1162, 1194
BbvI GCAGC 4 cut(s) 413, 454, 722, 1035
BccI CCATC 3 cut(s) 746, 875, 1245
BceAI ACGGC 1 cut(s) 39
BciT130I CCWGG 2 cut(s) 48, 201
BclI TGATCA 1 cut(s) 1222
BcnI CCSGG 1 cut(s) 682
BcoDI GTCTC 6 cut(s) 153, 338, 439, 689, 1057, 1188
BcuI ACTAGT 1 cut(s) 329
BfaI CTAG 1 cut(s) 330
BfmI CTRYAG 1 cut(s) 705
BisI GCNGC 5 cut(s) 402, 420, 443, 711, 1049
BlsI GCNGC 5 cut(s) 403, 421, 444, 712, 1050
Bme1390I CCNGG 3 cut(s) 48, 201, 682
Bme18I GGWCC 1 cut(s) 50
BmgT120I GGNCC 2 cut(s) 50, 794
BmiI GGNNCC 5 cut(s) 52, 164, 312, 579, 1020
BmrFI CCNGG 3 cut(s) 48, 201, 682
BmrI ACTGGG 2 cut(s) 279, 315
BmsI GCATC 3 cut(s) 755, 913, 1075
BmuI ACTGGG 2 cut(s) 279, 315
BpmI CTGGAG 1 cut(s) 713
BpuMI CCSGG 1 cut(s) 682
BsaI GGTCTC 2 cut(s) 439, 1057
BsaJI CCNNGG 3 cut(s) 407, 513, 681
BsaXI ACNNNNNCTCC 2 cut(s) 558, 588
Bsc4I CCNNNNNNNGG 4 cut(s) 89, 158, 497, 1028
Bse1I ACTGG 4 cut(s) 211, 274, 310, 530
Bse3DI GCAATG 2 cut(s) 507, 1148
BseBI CCWGG 2 cut(s) 48, 201
BseDI CCNNGG 3 cut(s) 407, 513, 681
BseGI GGATG 6 cut(s) 373, 523, 886, 985, 1141, 1162
BseLI CCNNNNNNNGG 4 cut(s) 89, 158, 497, 1028
BseMI GCAATG 2 cut(s) 507, 1148
BseMII CTCAG 5 cut(s) 121, 333, 429, 483, 888
BseNI ACTGG 4 cut(s) 211, 274, 310, 530
BseRI GAGGAG 1 cut(s) 570
BseSI GKGCMC 1 cut(s) 167
BseXI GCAGC 4 cut(s) 413, 454, 722, 1035
BseYI CCCAGC 1 cut(s) 791
Bsh1236I CGCG 1 cut(s) 391
BshFI GGCC 3 cut(s) 82, 796, 848
BshNI GGYRCC 1 cut(s) 162
BsiSI CCGG 1 cut(s) 681
BslFI GGGAC 1 cut(s) 615
BslI CCNNNNNNNGG 4 cut(s) 89, 158, 497, 1028
BsmAI GTCTC 6 cut(s) 153, 338, 439, 689, 1057, 1188
BsmFI GGGAC 1 cut(s) 615
BsmI GAATGC 1 cut(s) 542
BsnI GGCC 3 cut(s) 82, 796, 848
Bso31I GGTCTC 2 cut(s) 439, 1057
Bsp1286I GDGCHC 1 cut(s) 167
Bsp143I GATC 4 cut(s) 356, 411, 783, 1222
BspACI CCGC 3 cut(s) 70, 419, 773
BspANI GGCC 3 cut(s) 82, 796, 848
BspCNI CTCAG 5 cut(s) 120, 334, 428, 484, 887
BspFNI CGCG 1 cut(s) 391
BspLI GGNNCC 5 cut(s) 52, 164, 312, 579, 1020
BspPI GGATC 2 cut(s) 351, 419
BspQI GCTCTTC 1 cut(s) 681
BspT107I GGYRCC 1 cut(s) 162
BspTNI GGTCTC 2 cut(s) 439, 1057
BsrDI GCAATG 2 cut(s) 507, 1148
BsrI ACTGG 4 cut(s) 211, 274, 310, 530
BssECI CCNNGG 3 cut(s) 407, 513, 681
BssMI GATC 4 cut(s) 356, 411, 783, 1222
BssT1I CCWWGG 2 cut(s) 407, 513
Bst2UI CCWGG 2 cut(s) 48, 201
Bst4CI ACNGT 4 cut(s) 20, 295, 322, 853
Bst6I CTCTTC 1 cut(s) 681
BstC8I GCNNGC 2 cut(s) 944, 1053
BstDEI CTNAG 6 cut(s) 107, 342, 415, 492, 620, 874
BstENI CCTNNNNNAGG 1 cut(s) 495
BstF5I GGATG 6 cut(s) 373, 523, 886, 985, 1141, 1162
BstFNI CGCG 1 cut(s) 391
BstHHI GCGC 1 cut(s) 626
BstKTI GATC 4 cut(s) 359, 414, 786, 1225
BstMAI GTCTC 6 cut(s) 153, 338, 439, 689, 1057, 1188
BstMBI GATC 4 cut(s) 356, 411, 783, 1222
BstNI CCWGG 2 cut(s) 48, 201
BstNSI RCATGY 1 cut(s) 1283
BstSCI CCNGG 3 cut(s) 46, 199, 680
BstSFI CTRYAG 1 cut(s) 705
BstSLI GKGCMC 1 cut(s) 167
BstUI CGCG 1 cut(s) 391
BstV1I GCAGC 4 cut(s) 413, 454, 722, 1035
BstX2I RGATCY 2 cut(s) 356, 411
BstXI CCANNNNNNTGG 1 cut(s) 38
BstYI RGATCY 2 cut(s) 356, 411
BsuRI GGCC 3 cut(s) 82, 796, 848
BtsCI GGATG 6 cut(s) 373, 523, 886, 985, 1141, 1162
BtsI GCAGTG 1 cut(s) 43
BtsIMutI CAGTG 4 cut(s) 43, 115, 267, 537
Cac8I GCNNGC 2 cut(s) 944, 1053
CaiI CAGNNNCTG 1 cut(s) 422
CfoI GCGC 1 cut(s) 626
Cfr13I GGNCC 2 cut(s) 50, 794
Csp6I GTAC 5 cut(s) 21, 214, 569, 1011, 1103
CspCI CAANNNNNGTGG 2 cut(s) 882, 917
CviAII CATG 4 cut(s) 665, 714, 1014, 1280
CviQI GTAC 5 cut(s) 21, 214, 569, 1011, 1103
DdeI CTNAG 6 cut(s) 107, 342, 415, 492, 620, 874
DpnI GATC 4 cut(s) 358, 413, 785, 1224
DpnII GATC 4 cut(s) 356, 411, 783, 1222
DraIII CACNNNGTG 1 cut(s) 1172
EaeI YGGCCR 1 cut(s) 846
Eam1104I CTCTTC 1 cut(s) 681
EarI CTCTTC 1 cut(s) 681
EciI GGCGGA 1 cut(s) 762
Eco130I CCWWGG 2 cut(s) 407, 513
Eco31I GGTCTC 2 cut(s) 439, 1057
Eco32I GATATC 1 cut(s) 206
Eco47I GGWCC 1 cut(s) 50
Eco57I CTGAAG 1 cut(s) 1131
EcoNI CCTNNNNNAGG 1 cut(s) 495
EcoO109I RGGNCCY 1 cut(s) 50
EcoRI GAATTC 2 cut(s) 828, 998
EcoRII CCWGG 2 cut(s) 46, 199
EcoRV GATATC 1 cut(s) 206
EcoT14I CCWWGG 2 cut(s) 407, 513
EcoT22I ATGCAT 1 cut(s) 938
ErhI CCWWGG 2 cut(s) 407, 513
FaeI CATG 4 cut(s) 668, 717, 1017, 1283
FaqI GGGAC 1 cut(s) 615
FatI CATG 4 cut(s) 664, 713, 1013, 1279
FauI CCCGC 1 cut(s) 77
FauNDI CATATG 1 cut(s) 938
FbaI TGATCA 1 cut(s) 1222
Fnu4HI GCNGC 5 cut(s) 402, 420, 443, 711, 1049
FokI GGATG 6 cut(s) 380, 530, 893, 972, 1148, 1169
Fsp4HI GCNGC 5 cut(s) 402, 420, 443, 711, 1049
FspBI CTAG 1 cut(s) 330
GlaI GCGC 1 cut(s) 625
GluI GCNGC 5 cut(s) 402, 420, 443, 711, 1049
GsaI CCCAGC 1 cut(s) 795
GsuI CTGGAG 1 cut(s) 713
HaeIII GGCC 3 cut(s) 82, 796, 848
HapII CCGG 1 cut(s) 681
HhaI GCGC 1 cut(s) 626
Hin1II CATG 4 cut(s) 668, 717, 1017, 1283
Hin6I GCGC 1 cut(s) 624
HinP1I GCGC 1 cut(s) 624
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HindIII AAGCTT 1 cut(s) 1200
HinfI GANTC 7 cut(s) 494, 661, 697, 803, 1034, 1145, 1213
HpaII CCGG 1 cut(s) 681
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 2 cut(s) 41, 229
Hpy188I TCNGA 5 cut(s) 66, 827, 877, 953, 997
Hpy188III TCNNGA 4 cut(s) 341, 491, 921, 1167
Hpy8I GTNNAC 2 cut(s) 41, 229
HpyAV CCTTC 2 cut(s) 90, 1038
HpyCH4III ACNGT 4 cut(s) 20, 295, 322, 853
HpyCH4V TGCA 5 cut(s) 835, 904, 936, 942, 1208
HpyF3I CTNAG 6 cut(s) 107, 342, 415, 492, 620, 874
Hsp92II CATG 4 cut(s) 668, 717, 1017, 1283
HspAI GCGC 1 cut(s) 624
Ksp22I TGATCA 1 cut(s) 1222
Kzo9I GATC 4 cut(s) 356, 411, 783, 1222
LguI GCTCTTC 1 cut(s) 681
LmnI GCTCC 3 cut(s) 310, 409, 583
Lsp1109I GCAGC 4 cut(s) 413, 454, 722, 1035
LweI GCATC 3 cut(s) 755, 913, 1075
MaeI CTAG 1 cut(s) 330
MaeIII GTNAC 2 cut(s) 14, 265
MalI GATC 4 cut(s) 358, 413, 785, 1224
MboI GATC 4 cut(s) 356, 411, 783, 1222
MboII GAAGA 4 cut(s) 105, 698, 854, 951
MfeI CAATTG 1 cut(s) 1226
MflI RGATCY 2 cut(s) 356, 411
MhlI GDGCHC 1 cut(s) 167
MlsI TGGCCA 1 cut(s) 848
MluI ACGCGT 1 cut(s) 389
MluNI TGGCCA 1 cut(s) 848
MlyI GAGTC 3 cut(s) 503, 691, 1043
MmeI TCCRAC 2 cut(s) 89, 1006
Mox20I TGGCCA 1 cut(s) 848
Mph1103I ATGCAT 1 cut(s) 938
MscI TGGCCA 1 cut(s) 848
MseI TTAA 5 cut(s) 59, 194, 641, 731, 929
Msp20I TGGCCA 1 cut(s) 848
MspA1I CMGCKG 1 cut(s) 419
MspI CCGG 1 cut(s) 681
MspR9I CCNGG 3 cut(s) 48, 201, 682
MunI CAATTG 1 cut(s) 1226
Mva1269I GAATGC 1 cut(s) 542
MvaI CCWGG 2 cut(s) 48, 201
MvnI CGCG 1 cut(s) 391
NciI CCSGG 1 cut(s) 682
NdeI CATATG 1 cut(s) 938
NdeII GATC 4 cut(s) 356, 411, 783, 1222
NlaIII CATG 4 cut(s) 668, 717, 1017, 1283
NlaIV GGNNCC 5 cut(s) 52, 164, 312, 579, 1020
NmeAIII GCCGAG 1 cut(s) 418
NmuCI GTSAC 2 cut(s) 14, 265
NsiI ATGCAT 1 cut(s) 938
NspI RCATGY 1 cut(s) 1283
PciI ACATGT 1 cut(s) 1279
PciSI GCTCTTC 1 cut(s) 681
PctI GAATGC 1 cut(s) 542
PfeI GAWTC 4 cut(s) 661, 803, 1145, 1213
PflMI CCANNNNNTGG 1 cut(s) 1028
PfoI TCCNGGA 1 cut(s) 46
PkrI GCNGC 5 cut(s) 403, 421, 444, 712, 1050
PleI GAGTC 3 cut(s) 502, 691, 1042
PpsI GAGTC 3 cut(s) 502, 691, 1042
PpuMI RGGWCCY 1 cut(s) 50
PscI ACATGT 1 cut(s) 1279
Psp5II RGGWCCY 1 cut(s) 50
Psp6I CCWGG 2 cut(s) 46, 199
PspFI CCCAGC 1 cut(s) 791
PspGI CCWGG 2 cut(s) 46, 199
PspN4I GGNNCC 5 cut(s) 52, 164, 312, 579, 1020
PspPI GGNCC 2 cut(s) 50, 794
PspPPI RGGWCCY 1 cut(s) 50
PsrI GAACNNNNNNTAC 2 cut(s) 552, 584
PstNI CAGNNNCTG 1 cut(s) 422
PsuI RGATCY 2 cut(s) 356, 411
RsaI GTAC 5 cut(s) 22, 215, 570, 1012, 1104
RsaNI GTAC 5 cut(s) 21, 214, 569, 1011, 1103
SapI GCTCTTC 1 cut(s) 681
SaqAI TTAA 5 cut(s) 59, 194, 641, 731, 929
SatI GCNGC 5 cut(s) 402, 420, 443, 711, 1049
Sau3AI GATC 4 cut(s) 356, 411, 783, 1222
Sau96I GGNCC 2 cut(s) 50, 794
SchI GAGTC 3 cut(s) 503, 691, 1043
ScrFI CCNGG 3 cut(s) 48, 201, 682
SduI GDGCHC 1 cut(s) 167
SfaNI GCATC 3 cut(s) 755, 913, 1075
SfcI CTRYAG 1 cut(s) 705
SinI GGWCC 1 cut(s) 50
SpeI ACTAGT 1 cut(s) 329
SsiI CCGC 3 cut(s) 70, 419, 773
SspI AATATT 3 cut(s) 645, 927, 1027
SspMI CTAG 1 cut(s) 330
StyD4I CCNGG 3 cut(s) 46, 199, 680
StyI CCWWGG 2 cut(s) 407, 513
TaaI ACNGT 4 cut(s) 20, 295, 322, 853
TatI WGTACW 1 cut(s) 213
TauI GCSGC 1 cut(s) 422
TfiI GAWTC 4 cut(s) 661, 803, 1145, 1213
Tru1I TTAA 5 cut(s) 59, 194, 641, 731, 929
Tru9I TTAA 5 cut(s) 59, 194, 641, 731, 929
TscAI CASTG 4 cut(s) 43, 115, 274, 537
TseFI GTSAC 2 cut(s) 14, 265
TseI GCWGC 4 cut(s) 401, 442, 710, 1048
Tsp45I GTSAC 2 cut(s) 14, 265
TspDTI ATGAA 5 cut(s) 625, 854, 876, 1062, 1137
TspGWI ACGGA 2 cut(s) 246, 992
TspRI CASTG 4 cut(s) 43, 115, 274, 537
Van91I CCANNNNNTGG 1 cut(s) 1028
VpaK11BI GGWCC 1 cut(s) 50
XagI CCTNNNNNAGG 1 cut(s) 495
XapI RAATTY 5 cut(s) 673, 733, 828, 998, 1069
XceI RCATGY 1 cut(s) 1283
XspI CTAG 1 cut(s) 330
Zsp2I ATGCAT 1 cut(s) 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.