pycom12g20290

Sucrose nonfermenting 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001327
Physical Location & Seq
Forward (+)
21849132 .. 21853454
4323 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g20290.10

Sequence Viewer

Length: 1446 bp
ATGTTTGGGTCTGGTTCGGGTGCTGCACATCAAAGCAGTAGACTTTCAGTACCAGTTTTAATTCCGACGCGGTTTGTCTGGCCTTATGGCGGAAGGAGTGTGTTTCTCAGTGGTTCTTTCACAAGGTGGTTAGAACATATACCTATGGCTCCAGTGGAGGGGTGTCCAACTGTTTTTCAAGTTGTTTGGAATTTGACGCCCGGATATCATCAGTACAAATTTTTTGTTGATGGCCAATGGCGACATGATGAGCATCAGCCTTTTGTTAATGCGAATGGTGGAGTAGTGAATACCATCTTTCTAGCCGGAGAAGATATGGTTCCTACAAGTTTCAGTCCTGAGACTTCTGGTGGATCCAGTATGGACTTAGATGTGGTTCCTAGATATGTAGAAGCAGTTCCTAGGTTCTCTCAAGCTGATCTCGATATCTCTCGTCATCGTATATCTATTTTCTTGTCTAGACATACTGCTTATGAGTTGCTTCCCGAGTCAGGCAAGGTCATTGCCTTGGATAATAGTTTACCTGTGAAGCAAGCATTCCATATCCTGCACGAACAGGGATTACCTATGGCTCCTCTGTGGGATTTTGAGAAGGGCCAATTTGTTGGAGTTCTTAGCGCAATGGACTTCATCCTAATCCTGAGAGAGCTTGGGAATCATGGATCCAACTTGACTGAGGAAGAGCTTGAGACTCATACTGTAGCAGCATGGAAAGAGGGAAAAATAAATCTTAACAGACAACTTGATGACAATGGGAGATACCGTCCACCGCGTCTTGTCTCTGCTGGCCCTTATGATTCTCTCAAAGAAGTTGCTATGAAAATTTTGCAAAACAAGGTGGCAACAGTTCCAATTGTCCATTCAACTTCGCAGGATGGTTCATTTCCACAACTATTGCACCTTGCTTCCCTCTGCGGAATACTAAAATGCATATGCAGGCATTTTAGACATTCTTCTAGCTCCTTGCCCATACTCCTACATCCTATATCTGAGTTTCGTTTAGGCACATGGGTTCCAAATATTGGAGAGTCAAATGGACGGCCACTGGCAATGTTGAGACCAAATTCATCTCTTGCTGATGCCTTTTCTTTGTTGATACAAGCTGACGTTAGTTCAATACCTATAGTGGATGGTAATGATTCATTGCTGGATATTTATTCGCGAAGTGATATCACTGCTTTGGCCAGAGATAAAGCTTATACACAGATTCGCCTTGATGAATTGAGCATCCATCAGGCATTGCTACTGGGACAAAGTGAAAATCCTCCTTATGGCTTCTTTAATGGGCAGAGATGTCAGATGTGTTTGCAGTCAGATTCTTTGCATAAAGTGATGGAGCGCTTGGCTAATCCTGGGGTTAGGAGACTTGTGATTGTGGAGGCTGGCAGCAAGCGTGTAGAAGGGATTATTTCTTTGAGCGATGTATTCAGGTTCTTGCTAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000266 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006833 GO:0006950 GO:0006996 GO:0007005 GO:0007031 GO:0007154 GO:0008047 GO:0008150 GO:0008152 GO:0009267 GO:0009605 GO:0009856 GO:0009859 GO:0009875 GO:0009893 GO:0009987 GO:0009991 GO:0010562 GO:0010604 GO:0016043 GO:0016301 GO:0016310 GO:0016559 GO:0016740 GO:0016772 GO:0016773 GO:0019207 GO:0019209 GO:0019220 GO:0019222 GO:0019538 GO:0019887 GO:0022414 GO:0030234 GO:0030295 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0031667 GO:0031668 GO:0031669 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0033554 GO:0033674 GO:0036211 GO:0042044 GO:0042149 GO:0042325 GO:0042327 GO:0042594 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044706 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048285 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051716 GO:0060255 GO:0065007 GO:0065009 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0098772 GO:0140096 GO:1901564 GO:2000377
KEGG Pathways
Metabolic & Signaling
ko04068 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 map04068 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410
Pfam Domains
Protein Families
AMPK1_CBM CBS

Protein Analysis

482

Amino Acids

53.39

Weight (kDa)

6.31

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1022
AccI GTMKAC 1 cut(s) 40
AccII CGCG 3 cut(s) 70, 772, 1162
AciI CCGC 4 cut(s) 70, 90, 770, 915
AclWI GGATC 4 cut(s) 348, 361, 657, 670
AcoI YGGCCR 3 cut(s) 232, 1040, 1182
AcsI RAATTY 4 cut(s) 190, 218, 822, 1063
AcyI GRCGYC 1 cut(s) 197
AdeI CACNNNGTG 1 cut(s) 126
AfaI GTAC 2 cut(s) 51, 215
AfeI AGCGCT 1 cut(s) 1340
AfiI CCNNNNNNNGG 5 cut(s) 89, 158, 491, 1022, 1271
AgsI TTSAA 3 cut(s) 179, 864, 1116
AjnI CCWGG 1 cut(s) 1351
AjuI GAANNNNNNNTTGG 2 cut(s) 844, 876
AluBI AGCT 6 cut(s) 416, 649, 685, 960, 1103, 1196
AluI AGCT 6 cut(s) 416, 649, 685, 960, 1103, 1196
Alw26I GTCTC 5 cut(s) 335, 683, 784, 1051, 1357
AlwI GGATC 4 cut(s) 348, 361, 657, 670
Ama87I CYCGRG 1 cut(s) 485
Aor51HI AGCGCT 1 cut(s) 1340
AoxI GGCC 6 cut(s) 80, 232, 595, 787, 1040, 1182
ApeKI GCWGC 3 cut(s) 23, 704, 1386
ApoI RAATTY 4 cut(s) 190, 218, 822, 1063
Asp700I GAANNNNTTC 1 cut(s) 396
AspA2I CCTAGG 1 cut(s) 401
AspLEI GCGC 2 cut(s) 620, 1341
AspS9I GGNCC 2 cut(s) 595, 788
AsuC2I CCSGG 1 cut(s) 201
AvaI CYCGRG 1 cut(s) 485
AvrII CCTAGG 1 cut(s) 401
BalI TGGCCA 2 cut(s) 234, 1184
BamHI GGATCC 2 cut(s) 353, 662
BbvI GCAGC 3 cut(s) 10, 716, 1398
BccI CCATC 6 cut(s) 224, 302, 869, 1124, 1239, 1327
BceAI ACGGC 1 cut(s) 1055
BciT130I CCWGG 1 cut(s) 1353
BcnI CCSGG 1 cut(s) 201
BcoDI GTCTC 5 cut(s) 335, 683, 784, 1051, 1357
BfaI CTAG 7 cut(s) 302, 381, 402, 459, 957, 1439, 1444
BfmI CTRYAG 2 cut(s) 699, 1122
BfoI RGCGCY 1 cut(s) 1342
BisI GCNGC 3 cut(s) 24, 705, 1387
BlnI CCTAGG 1 cut(s) 401
BlsI GCNGC 3 cut(s) 25, 706, 1388
Bme1390I CCNGG 2 cut(s) 201, 1353
BmeT110I CYCGRG 1 cut(s) 485
BmgT120I GGNCC 2 cut(s) 595, 788
BmiI GGNNCC 7 cut(s) 150, 321, 355, 378, 573, 664, 1014
BmrFI CCNGG 2 cut(s) 201, 1353
BmrI ACTGGG 1 cut(s) 1256
BmsI GCATC 3 cut(s) 262, 1069, 1236
BmuI ACTGGG 1 cut(s) 1256
BpmI CTGGAG 1 cut(s) 135
BpuEI CTTGAG 2 cut(s) 396, 707
BpuMI CCSGG 1 cut(s) 201
BsaBI GATNNNNATC 1 cut(s) 252
BsaHI GRCGYC 1 cut(s) 197
BsaI GGTCTC 1 cut(s) 1051
BsaJI CCNNGG 3 cut(s) 401, 507, 1352
BsaXI ACNNNNNCTCC 2 cut(s) 748, 778
Bsc4I CCNNNNNNNGG 5 cut(s) 89, 158, 491, 1022, 1271
Bse1I ACTGG 5 cut(s) 53, 152, 357, 1050, 1251
Bse3DI GCAATG 5 cut(s) 501, 627, 1056, 1142, 1238
Bse8I GATNNNNATC 1 cut(s) 252
BseBI CCWGG 1 cut(s) 1353
BseDI CCNNGG 3 cut(s) 401, 507, 1352
BseGI GGATG 5 cut(s) 630, 880, 979, 1135, 1227
BseJI GATNNNNATC 1 cut(s) 252
BseLI CCNNNNNNNGG 5 cut(s) 89, 158, 491, 1022, 1271
BseMI GCAATG 5 cut(s) 501, 627, 1056, 1142, 1238
BseMII CTCAG 5 cut(s) 121, 330, 632, 666, 981
BseNI ACTGG 5 cut(s) 53, 152, 357, 1050, 1251
BseRI GAGGAG 1 cut(s) 564
BseXI GCAGC 3 cut(s) 10, 716, 1398
BsgI GTGCAG 2 cut(s) 9, 533
Bsh1236I CGCG 3 cut(s) 70, 772, 1162
BshFI GGCC 6 cut(s) 82, 234, 597, 789, 1042, 1184
BsiHKCI CYCGRG 1 cut(s) 485
BsiSI CCGG 2 cut(s) 201, 306
BslFI GGGAC 1 cut(s) 1263
BslI CCNNNNNNNGG 5 cut(s) 89, 158, 491, 1022, 1271
BsmAI GTCTC 5 cut(s) 335, 683, 784, 1051, 1357
BsmFI GGGAC 1 cut(s) 1263
BsmI GAATGC 1 cut(s) 536
BsnI GGCC 6 cut(s) 82, 234, 597, 789, 1042, 1184
Bso31I GGTCTC 1 cut(s) 1051
BsoBI CYCGRG 1 cut(s) 485
Bsp143I GATC 3 cut(s) 353, 418, 662
Bsp68I TCGCGA 1 cut(s) 1162
BspACI CCGC 4 cut(s) 70, 90, 770, 915
BspANI GGCC 6 cut(s) 82, 234, 597, 789, 1042, 1184
BspCNI CTCAG 5 cut(s) 120, 331, 633, 667, 982
BspFNI CGCG 3 cut(s) 70, 772, 1162
BspLI GGNNCC 7 cut(s) 150, 321, 355, 378, 573, 664, 1014
BspPI GGATC 4 cut(s) 348, 361, 657, 670
BspQI GCTCTTC 1 cut(s) 675
BspTNI GGTCTC 1 cut(s) 1051
BsrDI GCAATG 5 cut(s) 501, 627, 1056, 1142, 1238
BsrI ACTGG 5 cut(s) 53, 152, 357, 1050, 1251
BssECI CCNNGG 3 cut(s) 401, 507, 1352
BssMI GATC 3 cut(s) 353, 418, 662
BssNI GRCGYC 1 cut(s) 197
BssT1I CCWWGG 2 cut(s) 401, 507
Bst2UI CCWGG 1 cut(s) 1353
Bst4CI ACNGT 4 cut(s) 172, 700, 764, 847
Bst6I CTCTTC 1 cut(s) 675
BstACI GRCGYC 1 cut(s) 197
BstC8I GCNNGC 5 cut(s) 534, 787, 938, 1384, 1391
BstDEI CTNAG 7 cut(s) 107, 339, 367, 614, 641, 675, 990
BstF5I GGATG 5 cut(s) 630, 880, 979, 1135, 1227
BstFNI CGCG 3 cut(s) 70, 772, 1162
BstH2I RGCGCY 1 cut(s) 1342
BstHHI GCGC 2 cut(s) 620, 1341
BstKTI GATC 3 cut(s) 356, 421, 665
BstMAI GTCTC 5 cut(s) 335, 683, 784, 1051, 1357
BstMBI GATC 3 cut(s) 353, 418, 662
BstNI CCWGG 1 cut(s) 1353
BstSCI CCNGG 2 cut(s) 199, 1351
BstSFI CTRYAG 2 cut(s) 699, 1122
BstUI CGCG 3 cut(s) 70, 772, 1162
BstV1I GCAGC 3 cut(s) 10, 716, 1398
BstX2I RGATCY 2 cut(s) 353, 662
BstXI CCANNNNNNTGG 1 cut(s) 605
BstYI RGATCY 2 cut(s) 353, 662
BsuRI GGCC 6 cut(s) 82, 234, 597, 789, 1042, 1184
BtgZI GCGATG 1 cut(s) 1434
BtsCI GGATG 5 cut(s) 630, 880, 979, 1135, 1227
BtsI GCAGTG 1 cut(s) 1173
BtsIMutI CAGTG 4 cut(s) 115, 159, 1043, 1173
BtuMI TCGCGA 1 cut(s) 1162
Cac8I GCNNGC 5 cut(s) 534, 787, 938, 1384, 1391
CfoI GCGC 2 cut(s) 620, 1341
Cfr13I GGNCC 2 cut(s) 595, 788
CseI GACGC 3 cut(s) 76, 205, 761
Csp6I GTAC 2 cut(s) 50, 214
CspCI CAANNNNNGTGG 2 cut(s) 876, 911
CviAII CATG 4 cut(s) 245, 659, 708, 1008
CviQI GTAC 2 cut(s) 50, 214
DdeI CTNAG 7 cut(s) 107, 339, 367, 614, 641, 675, 990
DpnI GATC 3 cut(s) 355, 420, 664
DpnII GATC 3 cut(s) 353, 418, 662
DraIII CACNNNGTG 1 cut(s) 126
EaeI YGGCCR 3 cut(s) 232, 1040, 1182
Eam1104I CTCTTC 1 cut(s) 675
EarI CTCTTC 1 cut(s) 675
EciI GGCGGA 1 cut(s) 105
Eco130I CCWWGG 2 cut(s) 401, 507
Eco31I GGTCTC 1 cut(s) 1051
Eco32I GATATC 3 cut(s) 206, 427, 1171
Eco47III AGCGCT 1 cut(s) 1340
Eco88I CYCGRG 1 cut(s) 485
EcoRII CCWGG 1 cut(s) 1351
EcoRV GATATC 3 cut(s) 206, 427, 1171
EcoT14I CCWWGG 2 cut(s) 401, 507
EcoT22I ATGCAT 1 cut(s) 932
ErhI CCWWGG 2 cut(s) 401, 507
FaeI CATG 4 cut(s) 248, 662, 711, 1011
FaqI GGGAC 1 cut(s) 1263
FatI CATG 4 cut(s) 244, 658, 707, 1007
FauNDI CATATG 1 cut(s) 932
FblI GTMKAC 1 cut(s) 40
Fnu4HI GCNGC 3 cut(s) 24, 705, 1387
FokI GGATG 5 cut(s) 617, 887, 966, 1142, 1214
Fsp4HI GCNGC 3 cut(s) 24, 705, 1387
FspBI CTAG 7 cut(s) 302, 381, 402, 459, 957, 1439, 1444
GlaI GCGC 2 cut(s) 619, 1340
GluI GCNGC 3 cut(s) 24, 705, 1387
GsuI CTGGAG 1 cut(s) 135
HaeII RGCGCY 1 cut(s) 1342
HaeIII GGCC 6 cut(s) 82, 234, 597, 789, 1042, 1184
HapII CCGG 2 cut(s) 201, 306
HgaI GACGC 3 cut(s) 76, 205, 761
HhaI GCGC 2 cut(s) 620, 1341
Hin1I GRCGYC 1 cut(s) 197
Hin1II CATG 4 cut(s) 248, 662, 711, 1011
Hin6I GCGC 2 cut(s) 618, 1339
HinP1I GCGC 2 cut(s) 618, 1339
HindIII AAGCTT 1 cut(s) 1194
HinfI GANTC 8 cut(s) 488, 655, 691, 797, 1028, 1139, 1207, 1316
HpaII CCGG 2 cut(s) 201, 306
Hpy166II GTNNAC 3 cut(s) 41, 521, 767
Hpy188I TCNGA 4 cut(s) 66, 991, 1299, 1315
Hpy188III TCNNGA 6 cut(s) 338, 422, 459, 485, 640, 1161
Hpy8I GTNNAC 3 cut(s) 41, 521, 767
Hpy99I CGWCG 1 cut(s) 70
HpyAV CCTTC 3 cut(s) 87, 586, 1394
HpyCH4III ACNGT 4 cut(s) 172, 700, 764, 847
HpyCH4IV ACGT 1 cut(s) 1107
HpyCH4V TGCA 8 cut(s) 26, 550, 829, 898, 930, 936, 1309, 1324
HpyF3I CTNAG 7 cut(s) 107, 339, 367, 614, 641, 675, 990
HpySE526I ACGT 1 cut(s) 1107
Hsp92I GRCGYC 1 cut(s) 197
Hsp92II CATG 4 cut(s) 248, 662, 711, 1011
HspAI GCGC 2 cut(s) 618, 1339
Kzo9I GATC 3 cut(s) 353, 418, 662
LguI GCTCTTC 1 cut(s) 675
LmnI GCTCC 4 cut(s) 154, 577, 965, 1336
Lsp1109I GCAGC 3 cut(s) 10, 716, 1398
LweI GCATC 3 cut(s) 262, 1069, 1236
MaeI CTAG 7 cut(s) 302, 381, 402, 459, 957, 1439, 1444
MaeII ACGT 1 cut(s) 1107
MalI GATC 3 cut(s) 355, 420, 664
MboI GATC 3 cut(s) 353, 418, 662
MboII GAAGA 3 cut(s) 323, 692, 945
MfeI CAATTG 1 cut(s) 852
MflI RGATCY 2 cut(s) 353, 662
MlsI TGGCCA 2 cut(s) 234, 1184
MluCI AATT 8 cut(s) 60, 190, 218, 599, 822, 852, 1063, 1220
MluNI TGGCCA 2 cut(s) 234, 1184
MlyI GAGTC 3 cut(s) 497, 685, 1037
MmeI TCCRAC 4 cut(s) 89, 191, 586, 690
MnlI CCTC 7 cut(s) 151, 585, 670, 709, 920, 1275, 1372
Mox20I TGGCCA 2 cut(s) 234, 1184
Mph1103I ATGCAT 1 cut(s) 932
MroXI GAANNNNTTC 1 cut(s) 396
MscI TGGCCA 2 cut(s) 234, 1184
MseI TTAA 4 cut(s) 59, 267, 732, 1281
Msp20I TGGCCA 2 cut(s) 234, 1184
MspI CCGG 2 cut(s) 201, 306
MspR9I CCNGG 2 cut(s) 201, 1353
MunI CAATTG 1 cut(s) 852
Mva1269I GAATGC 1 cut(s) 536
MvaI CCWGG 1 cut(s) 1353
MvnI CGCG 3 cut(s) 70, 772, 1162
NciI CCSGG 1 cut(s) 201
NdeI CATATG 1 cut(s) 932
NdeII GATC 3 cut(s) 353, 418, 662
NlaIII CATG 4 cut(s) 248, 662, 711, 1011
NlaIV GGNNCC 7 cut(s) 150, 321, 355, 378, 573, 664, 1014
NruI TCGCGA 1 cut(s) 1162
NsiI ATGCAT 1 cut(s) 932
PciSI GCTCTTC 1 cut(s) 675
PctI GAATGC 1 cut(s) 536
PdmI GAANNNNTTC 1 cut(s) 396
PfeI GAWTC 5 cut(s) 655, 797, 1139, 1207, 1316
PflMI CCANNNNNTGG 1 cut(s) 1022
PkrI GCNGC 3 cut(s) 25, 706, 1388
PleI GAGTC 3 cut(s) 496, 685, 1036
PpsI GAGTC 3 cut(s) 496, 685, 1036
Psp6I CCWGG 1 cut(s) 1351
PspGI CCWGG 1 cut(s) 1351
PspN4I GGNNCC 7 cut(s) 150, 321, 355, 378, 573, 664, 1014
PspPI GGNCC 2 cut(s) 595, 788
PsrI GAACNNNNNNTAC 4 cut(s) 381, 413, 546, 578
PsuI RGATCY 2 cut(s) 353, 662
RruI TCGCGA 1 cut(s) 1162
RsaI GTAC 2 cut(s) 51, 215
RsaNI GTAC 2 cut(s) 50, 214
SapI GCTCTTC 1 cut(s) 675
SaqAI TTAA 4 cut(s) 59, 267, 732, 1281
SatI GCNGC 3 cut(s) 24, 705, 1387
Sau3AI GATC 3 cut(s) 353, 418, 662
Sau96I GGNCC 2 cut(s) 595, 788
SchI GAGTC 3 cut(s) 497, 685, 1037
ScrFI CCNGG 2 cut(s) 201, 1353
SfaNI GCATC 3 cut(s) 262, 1069, 1236
SfcI CTRYAG 2 cut(s) 699, 1122
SmlI CTYRAG 2 cut(s) 411, 686
SmoI CTYRAG 2 cut(s) 411, 686
Sse9I AATT 8 cut(s) 60, 190, 218, 599, 822, 852, 1063, 1220
SsiI CCGC 4 cut(s) 70, 90, 770, 915
SspI AATATT 1 cut(s) 1021
SspMI CTAG 7 cut(s) 302, 381, 402, 459, 957, 1439, 1444
StyD4I CCNGG 2 cut(s) 199, 1351
StyI CCWWGG 2 cut(s) 401, 507
TaaI ACNGT 4 cut(s) 172, 700, 764, 847
TaiI ACGT 1 cut(s) 1110
TaqI TCGA 1 cut(s) 423
TasI AATT 8 cut(s) 60, 190, 218, 599, 822, 852, 1063, 1220
TatI WGTACW 1 cut(s) 213
TfiI GAWTC 5 cut(s) 655, 797, 1139, 1207, 1316
Tru1I TTAA 4 cut(s) 59, 267, 732, 1281
Tru9I TTAA 4 cut(s) 59, 267, 732, 1281
TscAI CASTG 4 cut(s) 115, 159, 1050, 1180
TseI GCWGC 3 cut(s) 23, 704, 1386
TspDTI ATGAA 6 cut(s) 619, 833, 870, 1056, 1131, 1233
TspRI CASTG 4 cut(s) 115, 159, 1050, 1180
Van91I CCANNNNNTGG 1 cut(s) 1022
XapI RAATTY 4 cut(s) 190, 218, 822, 1063
XbaI TCTAGA 1 cut(s) 458
XmaJI CCTAGG 1 cut(s) 401
XmiI GTMKAC 1 cut(s) 40
XmnI GAANNNNTTC 1 cut(s) 396
XspI CTAG 7 cut(s) 302, 381, 402, 459, 957, 1439, 1444
Zsp2I ATGCAT 1 cut(s) 932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.