MD16G1172400.v1.1

Sucrose nonfermenting 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001327
Physical Location & Seq
Forward (+)
14509908 .. 14516800
6893 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1172400.v1.1.491

Sequence Viewer

Length: 1479 bp
ATGTTTGCTACCAACATGGATTCGGCGCGGGATGTTGGTGGAGCACCGAGCACGGTGTTGATCCCAATGCGTTTTGTGTGGCCTTATGGGGGCAGAAGTGTGTTTCTCAGTGGTTCATTTGCCCGATGGTCTGAACTTATTCCTATGACGCCAGTTGAGGGTTGCCCCACCGTGTTTCAAGCTATTTACAGCGTAACCCCTGGATACCACCAATACAAGTTTTTTGTTGATGGCGAATGGCGACATGATGAGCACCAACCTTTCGTGAGTGGTGAATATGGGTTAGTGAACACCATTCTCTTGGCTACAGATCCTAATCTCTTTCATCCGAATGTAACCCCAGAGATAACTTCTGGACCTAATTATATGGAAGAGGATAATGAGGCCTTTAGGCGTTTGGTCCGGATTACAGATGGTACTTTAACCGATGTACTACCAAGGATATCAGAGGTTGATTTGCAGAGCTCTCGTCATCGTATTTCTGTCTTCCTGTCTACACACACCACATATGAACTACTCCCCGAGTCAGGCAAGGTTGTTGCCTTGGATGTTGATTTACCTGTAAAGAAAGCGTTTCATATATTGCATGAGCAGGGAATCCCTTTGGCGCCTCTTTGGGACTTCAGCAATGGACGTTTTGTTGGAGTTATTAGCGCATTGGATTTTATTTTAATATTGAGAGAGCTTGGAAACCATGGATCTAATTTGACAGAGGAAGAGCTTGAAACACATACTATAGCTGCATGGAAAGAGGGAAAAGCATATCTAAATGGACAGGTTAATGGACATGGGAGAGCAGTGCCCAGACAGTTAGTCCATGCTGGACCATATGATAATATGAAAGATGTTGTTTTGAAAATTTTGCAAAATGGTTTTGCTACGGTTCCAATTATCCATTCATCTTCAGAAGATGGCTCATTTCCACAGCTATTACATCTTGCTTCACTGTCTGGTATTTTAAAATGTGTTTGCAGGTACTTTAGGCATTCATCTAGCTCATTACCCATACTTCAAGCACCAATATGTGCACTTAATGTGGGTACTTGGGTTCCAGAAATTGGAGAGTCGAATCGTCGGCCATTAGCAATGTTGAGACCAAGTGCTTCGCTTAGTGCAGCACTTAACTTGCTAGTTCAAGCTCAAGTAAGTTCAATACCTATAGTTGATGATACTAACTCTTTACTGGATATATATTGTCGAAGTGACATAACAGCTTTGGCTAAAGATAGAGCATATGCTCATATTAATCTCAATGAAATGAGTATTCAACGGGCATTGCAGTTGGGGCAAGACTCATTCTCTCCCTATGAACCAAGAGGTCAAAGATGTCAGATGTGTTTGCGCAGTGATTCTCTGCATAAAGTGATGGAGCGATTGGCCAATCCAGGTGTTAGACGACTTGTGATTGTAGAAGCTGGCAGTAAGCGGGTTGAAGGCATTGTTTCACTAAGTGACGTTTTCAAGTTCTTGCTCGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000266 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006833 GO:0006950 GO:0006996 GO:0007005 GO:0007031 GO:0007154 GO:0008047 GO:0008150 GO:0008152 GO:0009267 GO:0009605 GO:0009856 GO:0009859 GO:0009875 GO:0009893 GO:0009987 GO:0009991 GO:0010562 GO:0010604 GO:0016043 GO:0016301 GO:0016310 GO:0016559 GO:0016740 GO:0016772 GO:0016773 GO:0019207 GO:0019209 GO:0019220 GO:0019222 GO:0019538 GO:0019887 GO:0022414 GO:0030234 GO:0030295 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0031667 GO:0031668 GO:0031669 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0033554 GO:0033674 GO:0036211 GO:0042044 GO:0042149 GO:0042325 GO:0042327 GO:0042594 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044706 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048285 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051716 GO:0060255 GO:0065007 GO:0065009 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0098772 GO:0140096 GO:1901564 GO:2000377
KEGG Pathways
Metabolic & Signaling
ko04068 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 map04068 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410
Pfam Domains
Protein Families
AMPK1_CBM CBS

Protein Analysis

493

Amino Acids

54.54

Weight (kDa)

6.1

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMPK1_CBM PF16561 21 - 101 2.5e-23 Glycogen recognition site of AMP-activated protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1343
Acc36I ACCTGC 1 cut(s) 963
AccB1I GGYRCC 1 cut(s) 607
AccB7I CCANNNNNTGG 1 cut(s) 1058
AccI GTMKAC 1 cut(s) 494
AccII CGCG 1 cut(s) 28
AccIII TCCGGA 1 cut(s) 402
AciI CCGC 2 cut(s) 28, 1426
AclWI GGATC 3 cut(s) 55, 305, 706
AcoI YGGCCR 2 cut(s) 1076, 1377
AcsI RAATTY 1 cut(s) 858
AcuI CTGAAG 2 cut(s) 607, 888
AcyI GRCGYC 2 cut(s) 149, 608
AdeI CACNNNGTG 1 cut(s) 1451
AfaI GTAC 4 cut(s) 418, 432, 977, 1042
AfiI CCNNNNNNNGG 4 cut(s) 89, 158, 527, 1058
AgsI TTSAA 9 cut(s) 179, 725, 856, 1013, 1136, 1152, 1268, 1433, 1462
AhdI GACNNNNNGTC 1 cut(s) 812
AjnI CCWGG 2 cut(s) 199, 1384
AjuI GAANNNNNNNTTGG 2 cut(s) 880, 912
Alw21I GWGCWC 5 cut(s) 46, 53, 255, 467, 1030
Alw26I GTCTC 1 cut(s) 1087
Alw44I GTGCAC 1 cut(s) 1026
AlwI GGATC 3 cut(s) 55, 305, 706
Ama87I CYCGRG 1 cut(s) 521
Aor13HI TCCGGA 1 cut(s) 402
AoxI GGCC 4 cut(s) 80, 384, 1076, 1377
ApaLI GTGCAC 1 cut(s) 1026
ApeKI GCWGC 2 cut(s) 740, 1115
ApoI RAATTY 1 cut(s) 858
AseI ATTAAT 1 cut(s) 1245
Asp700I GAANNNNTTC 1 cut(s) 138
AspLEI GCGC 4 cut(s) 28, 610, 656, 1344
AspS9I GGNCC 3 cut(s) 356, 400, 824
AsuHPI GGTGA 1 cut(s) 284
AvaI CYCGRG 1 cut(s) 521
AvaII GGWCC 3 cut(s) 356, 400, 824
BaeGI GKGCMC 2 cut(s) 804, 1030
BaeI ACNNNNGTAYC 2 cut(s) 1032, 1065
BalI TGGCCA 1 cut(s) 1379
BanI GGYRCC 1 cut(s) 607
BanII GRGCYC 1 cut(s) 467
BbsI GAAGAC 1 cut(s) 478
Bbv12I GWGCWC 5 cut(s) 46, 53, 255, 467, 1030
BbvI GCAGC 2 cut(s) 727, 1127
BccI CCATC 5 cut(s) 120, 224, 407, 905, 1360
BcgI CGANNNNNNTGC 2 cut(s) 449, 483
BciT130I CCWGG 2 cut(s) 201, 1386
BciVI GTATCC 1 cut(s) 197
BcoDI GTCTC 1 cut(s) 1087
BfaI CTAG 2 cut(s) 993, 1130
BfmI CTRYAG 3 cut(s) 306, 735, 1158
BfoI RGCGCY 1 cut(s) 611
BfuAI ACCTGC 1 cut(s) 963
BfuI GTATCC 1 cut(s) 197
BisI GCNGC 2 cut(s) 741, 1116
BlsI GCNGC 2 cut(s) 742, 1117
Bme1390I CCNGG 2 cut(s) 201, 1386
Bme18I GGWCC 3 cut(s) 356, 400, 824
BmeRI GACNNNNNGTC 1 cut(s) 812
BmeT110I CYCGRG 1 cut(s) 521
BmgT120I GGNCC 3 cut(s) 356, 400, 824
BmiI GGNNCC 3 cut(s) 609, 885, 1050
BmrFI CCNGG 2 cut(s) 201, 1386
BpiI GAAGAC 1 cut(s) 478
BpuEI CTTGAG 1 cut(s) 1125
BsaBI GATNNNNATC 1 cut(s) 315
BsaHI GRCGYC 2 cut(s) 149, 608
BsaI GGTCTC 1 cut(s) 1087
BsaJI CCNNGG 4 cut(s) 199, 437, 543, 694
BsaWI WCCGGW 1 cut(s) 402
Bsc4I CCNNNNNNNGG 4 cut(s) 89, 158, 527, 1058
Bse1I ACTGG 2 cut(s) 152, 1188
Bse3DI GCAATG 3 cut(s) 634, 1092, 1274
Bse8I GATNNNNATC 1 cut(s) 315
BseAI TCCGGA 1 cut(s) 402
BseBI CCWGG 2 cut(s) 201, 1386
BseDI CCNNGG 4 cut(s) 199, 437, 543, 694
BseGI GGATG 3 cut(s) 37, 325, 553
BseJI GATNNNNATC 1 cut(s) 315
BseLI CCNNNNNNNGG 4 cut(s) 89, 158, 527, 1058
BseMI GCAATG 3 cut(s) 634, 1092, 1274
BseMII CTCAG 1 cut(s) 121
BseNI ACTGG 2 cut(s) 152, 1188
BseSI GKGCMC 2 cut(s) 804, 1030
BseXI GCAGC 2 cut(s) 727, 1127
BsgI GTGCAG 1 cut(s) 1134
Bsh1236I CGCG 1 cut(s) 28
BshFI GGCC 4 cut(s) 82, 386, 1078, 1379
BshNI GGYRCC 1 cut(s) 607
BsiHKAI GWGCWC 5 cut(s) 46, 53, 255, 467, 1030
BsiHKCI CYCGRG 1 cut(s) 521
BsiSI CCGG 1 cut(s) 403
BslFI GGGAC 1 cut(s) 632
BslI CCNNNNNNNGG 4 cut(s) 89, 158, 527, 1058
BsmAI GTCTC 1 cut(s) 1087
BsmFI GGGAC 1 cut(s) 632
BsmI GAATGC 1 cut(s) 985
BsnI GGCC 4 cut(s) 82, 386, 1078, 1379
Bso31I GGTCTC 1 cut(s) 1087
BsoBI CYCGRG 1 cut(s) 521
Bsp1286I GDGCHC 6 cut(s) 46, 53, 255, 467, 804, 1030
Bsp13I TCCGGA 1 cut(s) 402
Bsp143I GATC 3 cut(s) 60, 310, 698
Bsp19I CCATGG 1 cut(s) 694
BspACI CCGC 2 cut(s) 28, 1426
BspANI GGCC 4 cut(s) 82, 386, 1078, 1379
BspCNI CTCAG 1 cut(s) 120
BspEI TCCGGA 1 cut(s) 402
BspFNI CGCG 1 cut(s) 28
BspLI GGNNCC 3 cut(s) 609, 885, 1050
BspMI ACCTGC 1 cut(s) 963
BspPI GGATC 3 cut(s) 55, 305, 706
BspQI GCTCTTC 1 cut(s) 711
BspT107I GGYRCC 1 cut(s) 607
BspTNI GGTCTC 1 cut(s) 1087
BsrDI GCAATG 3 cut(s) 634, 1092, 1274
BsrI ACTGG 2 cut(s) 152, 1188
BssECI CCNNGG 4 cut(s) 199, 437, 543, 694
BssMI GATC 3 cut(s) 60, 310, 698
BssNI GRCGYC 2 cut(s) 149, 608
BssT1I CCWWGG 3 cut(s) 437, 543, 694
Bst2UI CCWGG 2 cut(s) 201, 1386
Bst4CI ACNGT 5 cut(s) 55, 172, 810, 883, 948
Bst6I CTCTTC 2 cut(s) 366, 711
BstACI GRCGYC 2 cut(s) 149, 608
BstC8I GCNNGC 1 cut(s) 1417
BstDEI CTNAG 3 cut(s) 107, 1109, 1448
BstDSI CCRYGG 1 cut(s) 694
BstF5I GGATG 3 cut(s) 37, 325, 553
BstFNI CGCG 1 cut(s) 28
BstH2I RGCGCY 1 cut(s) 611
BstHHI GCGC 4 cut(s) 28, 610, 656, 1344
BstKTI GATC 3 cut(s) 63, 313, 701
BstMAI GTCTC 1 cut(s) 1087
BstMBI GATC 3 cut(s) 60, 310, 698
BstMWI GCNNNNNNNGC 1 cut(s) 1285
BstNI CCWGG 2 cut(s) 201, 1386
BstSCI CCNGG 2 cut(s) 199, 1384
BstSFI CTRYAG 3 cut(s) 306, 735, 1158
BstSLI GKGCMC 2 cut(s) 804, 1030
BstUI CGCG 1 cut(s) 28
BstV1I GCAGC 2 cut(s) 727, 1127
BstV2I GAAGAC 1 cut(s) 478
BstX2I RGATCY 2 cut(s) 310, 698
BstXI CCANNNNNNTGG 1 cut(s) 301
BstYI RGATCY 2 cut(s) 310, 698
BsuI GTATCC 1 cut(s) 197
BsuRI GGCC 4 cut(s) 82, 386, 1078, 1379
BtgI CCRYGG 1 cut(s) 694
BtsCI GGATG 3 cut(s) 37, 325, 553
BtsI GCAGTG 2 cut(s) 804, 1351
BtsIMutI CAGTG 4 cut(s) 115, 804, 944, 1351
BveI ACCTGC 1 cut(s) 963
Cac8I GCNNGC 1 cut(s) 1417
CfoI GCGC 4 cut(s) 28, 610, 656, 1344
Cfr13I GGNCC 3 cut(s) 356, 400, 824
CseI GACGC 1 cut(s) 157
Csp6I GTAC 4 cut(s) 417, 431, 976, 1041
CviAII CATG 7 cut(s) 16, 245, 587, 695, 744, 788, 818
CviQI GTAC 4 cut(s) 417, 431, 976, 1041
DdeI CTNAG 3 cut(s) 107, 1109, 1448
DinI GGCGCC 1 cut(s) 609
DpnI GATC 3 cut(s) 62, 312, 700
DpnII GATC 3 cut(s) 60, 310, 698
DraI TTTAAA 1 cut(s) 960
DraIII CACNNNGTG 1 cut(s) 1451
DriI GACNNNNNGTC 1 cut(s) 812
EaeI YGGCCR 2 cut(s) 1076, 1377
Eam1104I CTCTTC 2 cut(s) 366, 711
Eam1105I GACNNNNNGTC 1 cut(s) 812
EarI CTCTTC 2 cut(s) 366, 711
Ecl136II GAGCTC 1 cut(s) 465
Eco130I CCWWGG 3 cut(s) 437, 543, 694
Eco147I AGGCCT 1 cut(s) 386
Eco24I GRGCYC 1 cut(s) 467
Eco31I GGTCTC 1 cut(s) 1087
Eco32I GATATC 1 cut(s) 444
Eco47I GGWCC 3 cut(s) 356, 400, 824
Eco53kI GAGCTC 1 cut(s) 465
Eco57I CTGAAG 2 cut(s) 607, 888
Eco88I CYCGRG 1 cut(s) 521
EcoICRI GAGCTC 1 cut(s) 465
EcoRII CCWGG 2 cut(s) 199, 1384
EcoRV GATATC 1 cut(s) 444
EcoT14I CCWWGG 3 cut(s) 437, 543, 694
EcoT38I GRGCYC 1 cut(s) 467
EgeI GGCGCC 1 cut(s) 609
EheI GGCGCC 1 cut(s) 609
ErhI CCWWGG 3 cut(s) 437, 543, 694
FaeI CATG 7 cut(s) 19, 248, 590, 698, 747, 791, 821
FaqI GGGAC 1 cut(s) 632
FatI CATG 7 cut(s) 15, 244, 586, 694, 743, 787, 817
FauI CCCGC 2 cut(s) 21, 1419
FauNDI CATATG 3 cut(s) 508, 829, 1234
FblI GTMKAC 1 cut(s) 494
Fnu4HI GCNGC 2 cut(s) 741, 1116
FokI GGATG 3 cut(s) 44, 312, 560
FriOI GRGCYC 1 cut(s) 467
Fsp4HI GCNGC 2 cut(s) 741, 1116
FspBI CTAG 2 cut(s) 993, 1130
FspI TGCGCA 1 cut(s) 1343
GlaI GCGC 4 cut(s) 27, 609, 655, 1343
GluI GCNGC 2 cut(s) 741, 1116
HaeII RGCGCY 1 cut(s) 611
HaeIII GGCC 4 cut(s) 82, 386, 1078, 1379
HapII CCGG 1 cut(s) 403
HgaI GACGC 1 cut(s) 157
HhaI GCGC 4 cut(s) 28, 610, 656, 1344
Hin1I GRCGYC 2 cut(s) 149, 608
Hin1II CATG 7 cut(s) 19, 248, 590, 698, 747, 791, 821
Hin6I GCGC 4 cut(s) 26, 608, 654, 1342
HinP1I GCGC 4 cut(s) 26, 608, 654, 1342
HinfI GANTC 7 cut(s) 20, 524, 597, 1064, 1069, 1292, 1349
HpaII CCGG 1 cut(s) 403
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 3 cut(s) 289, 495, 1028
Hpy188I TCNGA 5 cut(s) 133, 330, 448, 907, 1332
Hpy188III TCNNGA 4 cut(s) 265, 354, 403, 1052
Hpy8I GTNNAC 3 cut(s) 289, 495, 1028
Hpy99I CGWCG 1 cut(s) 1077
HpyAV CCTTC 1 cut(s) 1427
HpyCH4III ACNGT 5 cut(s) 55, 172, 810, 883, 948
HpyCH4IV ACGT 2 cut(s) 634, 1455
HpyCH4V TGCA 9 cut(s) 460, 586, 743, 865, 972, 1028, 1115, 1279, 1357
HpyF10VI GCNNNNNNNGC 1 cut(s) 1285
HpyF3I CTNAG 3 cut(s) 107, 1109, 1448
HpySE526I ACGT 2 cut(s) 634, 1455
Hsp92I GRCGYC 2 cut(s) 149, 608
Hsp92II CATG 7 cut(s) 19, 248, 590, 698, 747, 791, 821
HspAI GCGC 4 cut(s) 26, 608, 654, 1342
KasI GGCGCC 1 cut(s) 607
Kpn2I TCCGGA 1 cut(s) 402
Kzo9I GATC 3 cut(s) 60, 310, 698
LguI GCTCTTC 1 cut(s) 711
LmnI GCTCC 2 cut(s) 41, 1369
Lsp1109I GCAGC 2 cut(s) 727, 1127
MaeI CTAG 2 cut(s) 993, 1130
MaeII ACGT 2 cut(s) 634, 1455
MaeIII GTNAC 4 cut(s) 193, 334, 1202, 1451
MalI GATC 3 cut(s) 62, 312, 700
MboI GATC 3 cut(s) 60, 310, 698
MboII GAAGA 5 cut(s) 383, 478, 728, 894, 920
MflI RGATCY 2 cut(s) 310, 698
MhlI GDGCHC 6 cut(s) 46, 53, 255, 467, 804, 1030
MlsI TGGCCA 1 cut(s) 1379
MluCI AATT 5 cut(s) 361, 703, 858, 888, 1056
MluNI TGGCCA 1 cut(s) 1379
Mly113I GGCGCC 1 cut(s) 608
MlyI GAGTC 3 cut(s) 533, 1073, 1286
MmeI TCCRAC 1 cut(s) 622
MnlI CCTC 8 cut(s) 151, 367, 376, 442, 621, 706, 745, 1310
Mox20I TGGCCA 1 cut(s) 1379
MroI TCCGGA 1 cut(s) 402
MroXI GAANNNNTTC 1 cut(s) 138
MscI TGGCCA 1 cut(s) 1379
MseI TTAA 7 cut(s) 422, 671, 780, 959, 1032, 1122, 1245
MslI CAYNNNNRTG 2 cut(s) 330, 1021
Msp20I TGGCCA 1 cut(s) 1379
MspI CCGG 1 cut(s) 403
MspR9I CCNGG 2 cut(s) 201, 1386
Mva1269I GAATGC 1 cut(s) 985
MvaI CCWGG 2 cut(s) 201, 1386
MvnI CGCG 1 cut(s) 28
MwoI GCNNNNNNNGC 1 cut(s) 1285
NarI GGCGCC 1 cut(s) 608
NcoI CCATGG 1 cut(s) 694
NdeI CATATG 3 cut(s) 508, 829, 1234
NdeII GATC 3 cut(s) 60, 310, 698
NlaIII CATG 7 cut(s) 19, 248, 590, 698, 747, 791, 821
NlaIV GGNNCC 3 cut(s) 609, 885, 1050
NmuCI GTSAC 2 cut(s) 1202, 1451
NsbI TGCGCA 1 cut(s) 1343
PceI AGGCCT 1 cut(s) 386
PciSI GCTCTTC 1 cut(s) 711
PctI GAATGC 1 cut(s) 985
PdmI GAANNNNTTC 1 cut(s) 138
PfeI GAWTC 4 cut(s) 20, 597, 1069, 1349
PflMI CCANNNNNTGG 1 cut(s) 1058
PkrI GCNGC 2 cut(s) 742, 1117
PleI GAGTC 3 cut(s) 532, 1072, 1286
PluTI GGCGCC 1 cut(s) 611
PpsI GAGTC 3 cut(s) 532, 1072, 1286
PshBI ATTAAT 1 cut(s) 1245
Psp124BI GAGCTC 1 cut(s) 467
Psp6I CCWGG 2 cut(s) 199, 1384
PspGI CCWGG 2 cut(s) 199, 1384
PspN4I GGNNCC 3 cut(s) 609, 885, 1050
PspPI GGNCC 3 cut(s) 356, 400, 824
PsuI RGATCY 2 cut(s) 310, 698
RsaI GTAC 4 cut(s) 418, 432, 977, 1042
RsaNI GTAC 4 cut(s) 417, 431, 976, 1041
RseI CAYNNNNRTG 2 cut(s) 330, 1021
SacI GAGCTC 1 cut(s) 467
SapI GCTCTTC 1 cut(s) 711
SaqAI TTAA 7 cut(s) 422, 671, 780, 959, 1032, 1122, 1245
SatI GCNGC 2 cut(s) 741, 1116
Sau3AI GATC 3 cut(s) 60, 310, 698
Sau96I GGNCC 3 cut(s) 356, 400, 824
SchI GAGTC 3 cut(s) 533, 1073, 1286
ScrFI CCNGG 2 cut(s) 201, 1386
SduI GDGCHC 6 cut(s) 46, 53, 255, 467, 804, 1030
SfcI CTRYAG 3 cut(s) 306, 735, 1158
SfoI GGCGCC 1 cut(s) 609
SinI GGWCC 3 cut(s) 356, 400, 824
SmiMI CAYNNNNRTG 2 cut(s) 330, 1021
SmlI CTYRAG 1 cut(s) 1140
SmoI CTYRAG 1 cut(s) 1140
Sse9I AATT 5 cut(s) 361, 703, 858, 888, 1056
SseBI AGGCCT 1 cut(s) 386
SsiI CCGC 2 cut(s) 28, 1426
SspDI GGCGCC 1 cut(s) 607
SspI AATATT 1 cut(s) 675
SspMI CTAG 2 cut(s) 993, 1130
SstI GAGCTC 1 cut(s) 467
StuI AGGCCT 1 cut(s) 386
StyD4I CCNGG 2 cut(s) 199, 1384
StyI CCWWGG 3 cut(s) 437, 543, 694
TaaI ACNGT 5 cut(s) 55, 172, 810, 883, 948
TaiI ACGT 2 cut(s) 637, 1458
TaqI TCGA 2 cut(s) 1067, 1198
TasI AATT 5 cut(s) 361, 703, 858, 888, 1056
TatI WGTACW 1 cut(s) 430
TfiI GAWTC 4 cut(s) 20, 597, 1069, 1349
Tru1I TTAA 7 cut(s) 422, 671, 780, 959, 1032, 1122, 1245
Tru9I TTAA 7 cut(s) 422, 671, 780, 959, 1032, 1122, 1245
TscAI CASTG 4 cut(s) 115, 804, 951, 1351
TseFI GTSAC 2 cut(s) 1202, 1451
TseI GCWGC 2 cut(s) 740, 1115
Tsp45I GTSAC 2 cut(s) 1202, 1451
TspDTI ATGAA 9 cut(s) 105, 314, 525, 566, 854, 888, 978, 1269, 1323
TspRI CASTG 4 cut(s) 115, 804, 951, 1351
Van91I CCANNNNNTGG 1 cut(s) 1058
VneI GTGCAC 1 cut(s) 1026
VpaK11BI GGWCC 3 cut(s) 356, 400, 824
VspI ATTAAT 1 cut(s) 1245
XapI RAATTY 1 cut(s) 858
XmiI GTMKAC 1 cut(s) 494
XmnI GAANNNNTTC 1 cut(s) 138
XspI CTAG 2 cut(s) 993, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.