FvH4_4g19550

seed maturation protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009388
Physical Location & Seq
Reverse (-)
23182711 .. 23184645
1935 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g19550.t2

Sequence Viewer

Length: 873 bp
ATGCCATTTTTGGGTCTACTGGCCAGTCCAACACAAGTGACAATCACCTCCAAGTCTCCCACCAACTCTGCGAGCTTGAGATCGAAGCTGACCAACAAAAATGGACGTAGTCCCACCCAAGCCCGGGATGATCGACACGTGGCTCAAGAAGCACACTCTGATCCACACCGGCGCCACCCGGCACCCTTTCATCCTCGCCATCCGCGACGGCACCGTCGACATCTCCGCCTTCAAGCGCTGGCTGGTACCAAACGCCGTCGTTTATGTCCTCACCAAATTCTTTACCTTTTTTCTACAGTAGTTATAATTGGATTATGGATTGGTTTGCAGAGCCAGGATTATATATTTGTTCGAGCGTTTGTGCCGTTTGCGGCGAGTTTGGTGGTGAAAGGTTGGAAGAATTCTGATGATAGTGACGGTGACGTGGAAGTCATACTGAGTGGGCTTGCTGCTTTGAACGATGAGATTGACTGGTTCAAGAAACAAGCCTCCAAATGGGGTGTTGATCTTTCTGTTGTTGCTCCTCAGCAGCCTGCTCTAGATTACTGCAGATTTCTGGAGAATTTGACAAGTCCTGAGGTCGAGTACACAGTGGCCATGACGGCCTATTGGGCCATTGAAGCTGTGTACCAGGAGAGCTTTGCCCATTGCTTGGAGGAGGGATTCAAAACCCGACCGGAACTACAGGAGGTTTGTCACAGATGGGGCAATGATGGCTTTGGTAGCTACTGTTCTGCTATCCGAAGCATTGCTGACCGGCTATTGTTGAAGGCTTCAGATGATGAGGTGCGAAAAGCAGAAGTGACATTCCTGCGTGTTCTTGAATATGAGGTTGAATTCTGGAACATGAGCCGGGGGATTCCTAGGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00730 ko01100 map00730 map01100
Pfam Domains
Protein Families
TENA_THI-4

Protein Analysis

291

Amino Acids

33.11

Weight (kDa)

8.77

Isoelectric Point (pI)

60.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TENA_THI-4 PF03070 16 - 223 3.4e-40 TENA/THI-4/PQQC family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 305
AasI GACNNNNNNGTC 2 cut(s) 213, 428
Acc65I GGTACC 1 cut(s) 245
AccB1I GGYRCC 4 cut(s) 171, 181, 210, 245
AccB7I CCANNNNNTGG 1 cut(s) 652
AccI GTMKAC 2 cut(s) 16, 217
AccII CGCG 1 cut(s) 205
AciI CCGC 3 cut(s) 203, 226, 371
AclWI GGATC 1 cut(s) 155
AcoI YGGCCR 2 cut(s) 21, 594
AcsI RAATTY 4 cut(s) 276, 400, 562, 836
AcuI CTGAAG 1 cut(s) 759
AcvI CACGTG 1 cut(s) 139
AcyI GRCGYC 1 cut(s) 172
AfaI GTAC 4 cut(s) 247, 587, 629, 869
AfeI AGCGCT 1 cut(s) 237
AfiI CCNNNNNNNGG 5 cut(s) 11, 123, 124, 495, 652
AflIII ACRYGT 1 cut(s) 136
AgsI TTSAA 8 cut(s) 233, 457, 478, 620, 667, 769, 824, 836
AjiI CACGTC 1 cut(s) 424
AjnI CCWGG 2 cut(s) 333, 630
AluBI AGCT 5 cut(s) 75, 88, 623, 639, 726
AluI AGCT 5 cut(s) 75, 88, 623, 639, 726
Alw26I GTCTC 1 cut(s) 60
AlwI GGATC 1 cut(s) 155
Ama87I CYCGRG 1 cut(s) 123
Aor51HI AGCGCT 1 cut(s) 237
AoxI GGCC 4 cut(s) 21, 594, 603, 612
ApeKI GCWGC 2 cut(s) 449, 529
ApoI RAATTY 4 cut(s) 276, 400, 562, 836
Asp718I GGTACC 1 cut(s) 245
AspA2I CCTAGG 1 cut(s) 863
AspLEI GCGC 2 cut(s) 174, 238
AspS9I GGNCC 1 cut(s) 612
AsuC2I CCSGG 4 cut(s) 124, 125, 179, 854
AsuHPI GGTGA 4 cut(s) 37, 263, 397, 431
AvaI CYCGRG 1 cut(s) 123
AvrII CCTAGG 1 cut(s) 863
AxyI CCTNAGG 1 cut(s) 576
BalI TGGCCA 2 cut(s) 23, 596
BanI GGYRCC 4 cut(s) 171, 181, 210, 245
BbrPI CACGTG 1 cut(s) 139
BbvCI CCTCAGC 1 cut(s) 525
BbvI GCAGC 2 cut(s) 436, 541
BccI CCATC 3 cut(s) 207, 696, 707
BceAI ACGGC 4 cut(s) 224, 240, 349, 618
BciT130I CCWGG 2 cut(s) 335, 632
BcnI CCSGG 4 cut(s) 124, 125, 179, 854
BcoDI GTCTC 1 cut(s) 60
BfaI CTAG 2 cut(s) 539, 864
BfmI CTRYAG 3 cut(s) 294, 547, 683
BfoI RGCGCY 2 cut(s) 175, 239
BglI GCCNNNNNGGC 2 cut(s) 602, 611
BisI GCNGC 3 cut(s) 372, 450, 530
BlnI CCTAGG 1 cut(s) 863
BlsI GCNGC 3 cut(s) 373, 451, 531
Bme1390I CCNGG 6 cut(s) 124, 125, 179, 335, 632, 854
BmeT110I CYCGRG 1 cut(s) 123
BmgBI CACGTC 1 cut(s) 424
BmgT120I GGNCC 1 cut(s) 612
BmiI GGNNCC 4 cut(s) 173, 183, 212, 247
BmrFI CCNGG 6 cut(s) 124, 125, 179, 335, 632, 854
BpmI CTGGAG 1 cut(s) 578
Bpu10I CCTNAGC 1 cut(s) 525
BpuEI CTTGAG 2 cut(s) 97, 129
BpuMI CCSGG 4 cut(s) 124, 125, 179, 854
BsaAI YACGTR 1 cut(s) 139
BsaHI GRCGYC 1 cut(s) 172
BsaJI CCNNGG 3 cut(s) 123, 853, 863
BsaWI WCCGGW 1 cut(s) 676
Bsc4I CCNNNNNNNGG 5 cut(s) 11, 123, 124, 495, 652
Bse118I RCCGGY 2 cut(s) 168, 756
Bse1I ACTGG 3 cut(s) 24, 24, 476
Bse21I CCTNAGG 1 cut(s) 576
Bse3DI GCAATG 3 cut(s) 646, 715, 747
BseBI CCWGG 2 cut(s) 335, 632
BseDI CCNNGG 3 cut(s) 123, 853, 863
BseGI GGATG 3 cut(s) 133, 190, 199
BseLI CCNNNNNNNGG 5 cut(s) 11, 123, 124, 495, 652
BseMI GCAATG 3 cut(s) 646, 715, 747
BseMII CTCAG 3 cut(s) 428, 539, 567
BseNI ACTGG 3 cut(s) 24, 24, 476
BseRI GAGGAG 2 cut(s) 513, 671
BseXI GCAGC 2 cut(s) 436, 541
Bsh1236I CGCG 1 cut(s) 205
Bsh1285I CGRYCG 1 cut(s) 677
BshFI GGCC 4 cut(s) 23, 596, 605, 614
BshNI GGYRCC 4 cut(s) 171, 181, 210, 245
BsiEI CGRYCG 1 cut(s) 677
BsiHKCI CYCGRG 1 cut(s) 123
BsiSI CCGG 6 cut(s) 124, 169, 179, 677, 757, 853
BslFI GGGAC 1 cut(s) 96
BslI CCNNNNNNNGG 5 cut(s) 11, 123, 124, 495, 652
BsmAI GTCTC 1 cut(s) 60
BsmFI GGGAC 1 cut(s) 96
BsnI GGCC 4 cut(s) 23, 596, 605, 614
BsoBI CYCGRG 1 cut(s) 123
Bsp143I GATC 4 cut(s) 80, 130, 160, 505
BspACI CCGC 3 cut(s) 203, 226, 371
BspANI GGCC 4 cut(s) 23, 596, 605, 614
BspCNI CTCAG 3 cut(s) 429, 538, 568
BspFNI CGCG 1 cut(s) 205
BspLI GGNNCC 4 cut(s) 173, 183, 212, 247
BspMAI CTGCAG 1 cut(s) 551
BspPI GGATC 1 cut(s) 155
BspT107I GGYRCC 4 cut(s) 171, 181, 210, 245
BsrDI GCAATG 3 cut(s) 646, 715, 747
BsrFI RCCGGY 2 cut(s) 168, 756
BsrI ACTGG 3 cut(s) 24, 24, 476
BssAI RCCGGY 2 cut(s) 168, 756
BssECI CCNNGG 3 cut(s) 123, 853, 863
BssMI GATC 4 cut(s) 80, 130, 160, 505
BssNI GRCGYC 1 cut(s) 172
BssT1I CCWWGG 1 cut(s) 863
Bst2UI CCWGG 2 cut(s) 335, 632
Bst4CI ACNGT 5 cut(s) 215, 298, 419, 592, 731
BstACI GRCGYC 1 cut(s) 172
BstBAI YACGTR 1 cut(s) 139
BstC8I GCNNGC 4 cut(s) 73, 240, 447, 534
BstDEI CTNAG 3 cut(s) 437, 525, 576
BstF5I GGATG 3 cut(s) 133, 190, 199
BstFNI CGCG 1 cut(s) 205
BstH2I RGCGCY 2 cut(s) 175, 239
BstHHI GCGC 2 cut(s) 174, 238
BstKTI GATC 4 cut(s) 83, 133, 163, 508
BstMAI GTCTC 1 cut(s) 60
BstMBI GATC 4 cut(s) 80, 130, 160, 505
BstMCI CGRYCG 1 cut(s) 677
BstMWI GCNNNNNNNGC 6 cut(s) 149, 602, 611, 620, 714, 723
BstNI CCWGG 2 cut(s) 335, 632
BstSCI CCNGG 6 cut(s) 122, 123, 177, 333, 630, 852
BstSFI CTRYAG 3 cut(s) 294, 547, 683
BstUI CGCG 1 cut(s) 205
BstV1I GCAGC 2 cut(s) 436, 541
Bsu36I CCTNAGG 1 cut(s) 576
BsuRI GGCC 4 cut(s) 23, 596, 605, 614
BtrI CACGTC 1 cut(s) 424
BtsCI GGATG 3 cut(s) 133, 190, 199
BtsIMutI CAGTG 1 cut(s) 597
Cac8I GCNNGC 4 cut(s) 73, 240, 447, 534
CfoI GCGC 2 cut(s) 174, 238
Cfr10I RCCGGY 2 cut(s) 168, 756
Cfr13I GGNCC 1 cut(s) 612
Cfr9I CCCGGG 1 cut(s) 123
Csp6I GTAC 4 cut(s) 246, 586, 628, 868
CviAII CATG 2 cut(s) 598, 847
CviQI GTAC 4 cut(s) 246, 586, 628, 868
DdeI CTNAG 3 cut(s) 437, 525, 576
DinI GGCGCC 1 cut(s) 173
DpnI GATC 4 cut(s) 82, 132, 162, 507
DpnII GATC 4 cut(s) 80, 130, 160, 505
DrdI GACNNNNNNGTC 2 cut(s) 213, 428
DseDI GACNNNNNNGTC 2 cut(s) 213, 428
EaeI YGGCCR 2 cut(s) 21, 594
EciI GGCGGA 1 cut(s) 215
Eco130I CCWWGG 1 cut(s) 863
Eco47III AGCGCT 1 cut(s) 237
Eco57I CTGAAG 1 cut(s) 759
Eco72I CACGTG 1 cut(s) 139
Eco81I CCTNAGG 1 cut(s) 576
Eco88I CYCGRG 1 cut(s) 123
EcoRI GAATTC 2 cut(s) 400, 836
EcoRII CCWGG 2 cut(s) 333, 630
EcoT14I CCWWGG 1 cut(s) 863
EgeI GGCGCC 1 cut(s) 173
EheI GGCGCC 1 cut(s) 173
ErhI CCWWGG 1 cut(s) 863
FaeI CATG 2 cut(s) 601, 850
FaiI YATR 9 cut(s) 265, 305, 316, 342, 344, 434, 599, 828, 848
FaqI GGGAC 1 cut(s) 96
FatI CATG 2 cut(s) 597, 846
FblI GTMKAC 2 cut(s) 16, 217
Fnu4HI GCNGC 3 cut(s) 372, 450, 530
FokI GGATG 3 cut(s) 140, 177, 186
Fsp4HI GCNGC 3 cut(s) 372, 450, 530
FspBI CTAG 2 cut(s) 539, 864
GlaI GCGC 2 cut(s) 173, 237
GluI GCNGC 3 cut(s) 372, 450, 530
GsuI CTGGAG 1 cut(s) 578
HaeII RGCGCY 2 cut(s) 175, 239
HaeIII GGCC 4 cut(s) 23, 596, 605, 614
HapII CCGG 6 cut(s) 124, 169, 179, 677, 757, 853
HhaI GCGC 2 cut(s) 174, 238
Hin1I GRCGYC 1 cut(s) 172
Hin1II CATG 2 cut(s) 601, 850
Hin6I GCGC 2 cut(s) 172, 236
HinP1I GCGC 2 cut(s) 172, 236
HincII GTYRAC 1 cut(s) 218
HindII GTYRAC 1 cut(s) 218
HinfI GANTC 2 cut(s) 663, 859
HpaII CCGG 6 cut(s) 124, 169, 179, 677, 757, 853
HphI GGTGA 4 cut(s) 37, 263, 397, 431
Hpy166II GTNNAC 4 cut(s) 17, 218, 588, 628
Hpy188I TCNGA 4 cut(s) 160, 406, 743, 778
Hpy188III TCNNGA 7 cut(s) 146, 478, 539, 557, 575, 821, 841
Hpy8I GTNNAC 4 cut(s) 17, 218, 588, 628
Hpy99I CGWCG 3 cut(s) 210, 219, 261
HpyAV CCTTC 2 cut(s) 239, 763
HpyCH4III ACNGT 5 cut(s) 215, 298, 419, 592, 731
HpyCH4IV ACGT 3 cut(s) 106, 138, 423
HpyCH4V TGCA 2 cut(s) 328, 549
HpyF10VI GCNNNNNNNGC 6 cut(s) 149, 602, 611, 620, 714, 723
HpyF3I CTNAG 3 cut(s) 437, 525, 576
HpySE526I ACGT 3 cut(s) 106, 138, 423
Hsp92I GRCGYC 1 cut(s) 172
Hsp92II CATG 2 cut(s) 601, 850
HspAI GCGC 2 cut(s) 172, 236
KasI GGCGCC 1 cut(s) 171
KpnI GGTACC 1 cut(s) 249
Kzo9I GATC 4 cut(s) 80, 130, 160, 505
LmnI GCTCC 1 cut(s) 526
Lsp1109I GCAGC 2 cut(s) 436, 541
MaeI CTAG 2 cut(s) 539, 864
MaeII ACGT 3 cut(s) 106, 138, 423
MaeIII GTNAC 5 cut(s) 37, 413, 419, 695, 802
MalI GATC 4 cut(s) 82, 132, 162, 507
MboI GATC 4 cut(s) 80, 130, 160, 505
MboII GAAGA 1 cut(s) 409
MlsI TGGCCA 2 cut(s) 23, 596
MluCI AATT 5 cut(s) 276, 306, 400, 562, 836
MluNI TGGCCA 2 cut(s) 23, 596
Mly113I GGCGCC 1 cut(s) 172
MmeI TCCRAC 2 cut(s) 53, 374
Mox20I TGGCCA 2 cut(s) 23, 596
MscI TGGCCA 2 cut(s) 23, 596
Msp20I TGGCCA 2 cut(s) 23, 596
MspI CCGG 6 cut(s) 124, 169, 179, 677, 757, 853
MspR9I CCNGG 6 cut(s) 124, 125, 179, 335, 632, 854
MvaI CCWGG 2 cut(s) 335, 632
MvnI CGCG 1 cut(s) 205
MwoI GCNNNNNNNGC 6 cut(s) 149, 602, 611, 620, 714, 723
NarI GGCGCC 1 cut(s) 172
NciI CCSGG 4 cut(s) 124, 125, 179, 854
NdeII GATC 4 cut(s) 80, 130, 160, 505
NlaIII CATG 2 cut(s) 601, 850
NlaIV GGNNCC 4 cut(s) 173, 183, 212, 247
NmuCI GTSAC 5 cut(s) 37, 413, 419, 695, 802
PcsI WCGNNNNNNNCGW 2 cut(s) 202, 214
PfeI GAWTC 2 cut(s) 663, 859
PflFI GACNNNGTC 1 cut(s) 108
PflMI CCANNNNNTGG 1 cut(s) 652
PkrI GCNGC 3 cut(s) 373, 451, 531
PluTI GGCGCC 1 cut(s) 175
PmaCI CACGTG 1 cut(s) 139
PmlI CACGTG 1 cut(s) 139
Ppu21I YACGTR 1 cut(s) 139
PsiI TTATAA 1 cut(s) 305
Psp6I CCWGG 2 cut(s) 333, 630
PspCI CACGTG 1 cut(s) 139
PspGI CCWGG 2 cut(s) 333, 630
PspN4I GGNNCC 4 cut(s) 173, 183, 212, 247
PspPI GGNCC 1 cut(s) 612
PsrI GAACNNNNNNTAC 2 cut(s) 715, 747
PstI CTGCAG 1 cut(s) 551
PsyI GACNNNGTC 1 cut(s) 108
RsaI GTAC 4 cut(s) 247, 587, 629, 869
RsaNI GTAC 4 cut(s) 246, 586, 628, 868
SalI GTCGAC 1 cut(s) 216
SatI GCNGC 3 cut(s) 372, 450, 530
Sau3AI GATC 4 cut(s) 80, 130, 160, 505
Sau96I GGNCC 1 cut(s) 612
ScrFI CCNGG 6 cut(s) 124, 125, 179, 335, 632, 854
SfcI CTRYAG 3 cut(s) 294, 547, 683
SfiI GGCCNNNNNGGCC 2 cut(s) 602, 611
SfoI GGCGCC 1 cut(s) 173
SgrAI CRCCGGYG 1 cut(s) 168
SmaI CCCGGG 1 cut(s) 125
SmlI CTYRAG 2 cut(s) 76, 144
SmoI CTYRAG 2 cut(s) 76, 144
Sse9I AATT 5 cut(s) 276, 306, 400, 562, 836
SsiI CCGC 3 cut(s) 203, 226, 371
SspDI GGCGCC 1 cut(s) 171
SspMI CTAG 2 cut(s) 539, 864
StyD4I CCNGG 6 cut(s) 122, 123, 177, 333, 630, 852
StyI CCWWGG 1 cut(s) 863
TaaI ACNGT 5 cut(s) 215, 298, 419, 592, 731
TaiI ACGT 3 cut(s) 109, 141, 426
TaqI TCGA 5 cut(s) 83, 133, 217, 352, 582
TasI AATT 5 cut(s) 276, 306, 400, 562, 836
TatI WGTACW 1 cut(s) 585
TauI GCSGC 1 cut(s) 374
TfiI GAWTC 2 cut(s) 663, 859
TscAI CASTG 1 cut(s) 597
TseFI GTSAC 5 cut(s) 37, 413, 419, 695, 802
TseI GCWGC 2 cut(s) 449, 529
Tsp45I GTSAC 5 cut(s) 37, 413, 419, 695, 802
TspDTI ATGAA 1 cut(s) 179
TspMI CCCGGG 1 cut(s) 123
TspRI CASTG 1 cut(s) 597
Tth111I GACNNNGTC 1 cut(s) 108
Van91I CCANNNNNTGG 1 cut(s) 652
XapI RAATTY 4 cut(s) 276, 400, 562, 836
XbaI TCTAGA 1 cut(s) 538
XmaI CCCGGG 1 cut(s) 123
XmaJI CCTAGG 1 cut(s) 863
XmiI GTMKAC 2 cut(s) 16, 217
XspI CTAG 2 cut(s) 539, 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.