Rh4AG256900

seed maturation protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0009388
Physical Location & Seq
Reverse (-)
57182967 .. 57185164
2198 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG256900.1

Sequence Viewer

Length: 570 bp
ATGATTGCTGTTTTGAATTGGTTGCAGAGCCAGGACTACATATTTGTGAGAGCTTTTGTGCCGTTTGTGGCGAGCTTGGTGGTGAAAGCTTGGAAGAAGTCTGATGATAGTCATGGTGATGTGGAAGTGATACTGAGTGGGCTTGCTGCTTTGAACGATGAGATTGACTGGTTCAAGAAACAAGCCTCCAAATGGGGTGTTGATCTCTCTGTTGTTGCTCCTCAAAAGCCTACCCAGGATTACTGCAGATTTCTGGAGAATTTGACTAGTCCTGAGGTTGATTACACAGTGGGCATGACGGCCTATTGGGCCATTGAAGCTGTGTACCAAGAGAGCTTTGCCCATTGCCTGGGGGAGGGCTCCAAAACCCCACCGGAACTAGAAGAGGTTTGTCAAAGATGGGGCAATGATGGCTTCGGTAACTACTGTTCTGCTCTCCGAAGCATTGCTGACCGGCGTTTGTTGAAGGCCACAGATGATGAGGTGAGTAAAGCTGAAGTGACATTCCTGCGTGTTCTTGAATATGAGGTTGAGTTCTGGAACATGAGCCGTGGGATTCCTGGGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00730 ko01100 map00730 map01100
Pfam Domains
Protein Families
TENA_THI-4

Protein Analysis

189

Amino Acids

21.43

Weight (kDa)

4.68

Isoelectric Point (pI)

39.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TENA_THI-4 PF03070 11 - 184 1.4e-28 TENA/THI-4/PQQC family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 349
AcsI RAATTY 1 cut(s) 259
AcuI CTGAAG 1 cut(s) 516
AfaI GTAC 2 cut(s) 326, 566
AfiI CCNNNNNNNGG 3 cut(s) 192, 349, 355
AgsI TTSAA 6 cut(s) 16, 154, 175, 317, 466, 521
AhlI ACTAGT 1 cut(s) 266
AjnI CCWGG 4 cut(s) 30, 234, 348, 559
AluBI AGCT 6 cut(s) 53, 75, 89, 320, 336, 494
AluI AGCT 6 cut(s) 53, 75, 89, 320, 336, 494
AoxI GGCC 3 cut(s) 300, 309, 468
ApeKI GCWGC 1 cut(s) 146
ApoI RAATTY 1 cut(s) 259
ArsI GACNNNNNNTTYG 2 cut(s) 26, 58
AspS9I GGNCC 1 cut(s) 309
AsuHPI GGTGA 3 cut(s) 94, 128, 496
AxyI CCTNAGG 1 cut(s) 273
BanII GRGCYC 1 cut(s) 362
BbvI GCAGC 1 cut(s) 133
BccI CCATC 2 cut(s) 393, 404
BceAI ACGGC 3 cut(s) 46, 315, 534
BciT130I CCWGG 4 cut(s) 32, 236, 350, 561
BcuI ACTAGT 1 cut(s) 266
BfaI CTAG 3 cut(s) 267, 380, 568
BfmI CTRYAG 1 cut(s) 244
BglI GCCNNNNNGGC 1 cut(s) 308
BisI GCNGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 148
Bme1390I CCNGG 4 cut(s) 32, 236, 350, 561
BmgT120I GGNCC 1 cut(s) 309
BmiI GGNNCC 1 cut(s) 361
BmrFI CCNGG 4 cut(s) 32, 236, 350, 561
BpmI CTGGAG 1 cut(s) 275
BsaJI CCNNGG 4 cut(s) 234, 349, 550, 560
BsaWI WCCGGW 1 cut(s) 373
Bsc4I CCNNNNNNNGG 3 cut(s) 192, 349, 355
Bse118I RCCGGY 1 cut(s) 453
Bse1I ACTGG 1 cut(s) 173
Bse21I CCTNAGG 1 cut(s) 273
Bse3DI GCAATG 3 cut(s) 343, 412, 444
BseBI CCWGG 4 cut(s) 32, 236, 350, 561
BseDI CCNNGG 4 cut(s) 234, 349, 550, 560
BseLI CCNNNNNNNGG 3 cut(s) 192, 349, 355
BseMI GCAATG 3 cut(s) 343, 412, 444
BseMII CTCAG 2 cut(s) 125, 264
BseNI ACTGG 1 cut(s) 173
BseRI GAGGAG 1 cut(s) 210
BseXI GCAGC 1 cut(s) 133
BshFI GGCC 3 cut(s) 302, 311, 470
BsiSI CCGG 2 cut(s) 374, 454
BslI CCNNNNNNNGG 3 cut(s) 192, 349, 355
BsnI GGCC 3 cut(s) 302, 311, 470
Bsp1286I GDGCHC 1 cut(s) 362
Bsp143I GATC 1 cut(s) 202
BspANI GGCC 3 cut(s) 302, 311, 470
BspCNI CTCAG 2 cut(s) 126, 265
BspLI GGNNCC 1 cut(s) 361
BspMAI CTGCAG 1 cut(s) 248
BsrDI GCAATG 3 cut(s) 343, 412, 444
BsrFI RCCGGY 1 cut(s) 453
BsrI ACTGG 1 cut(s) 173
BssAI RCCGGY 1 cut(s) 453
BssECI CCNNGG 4 cut(s) 234, 349, 550, 560
BssMI GATC 1 cut(s) 202
Bst2UI CCWGG 4 cut(s) 32, 236, 350, 561
Bst4CI ACNGT 2 cut(s) 289, 428
Bst6I CTCTTC 1 cut(s) 378
BstC8I GCNNGC 2 cut(s) 73, 144
BstDEI CTNAG 2 cut(s) 134, 273
BstDSI CCRYGG 1 cut(s) 550
BstENI CCTNNNNNAGG 1 cut(s) 353
BstKTI GATC 1 cut(s) 205
BstMBI GATC 1 cut(s) 202
BstMWI GCNNNNNNNGC 3 cut(s) 308, 317, 411
BstNI CCWGG 4 cut(s) 32, 236, 350, 561
BstSCI CCNGG 4 cut(s) 30, 234, 348, 559
BstSFI CTRYAG 1 cut(s) 244
BstV1I GCAGC 1 cut(s) 133
Bsu36I CCTNAGG 1 cut(s) 273
BsuRI GGCC 3 cut(s) 302, 311, 470
BtgI CCRYGG 1 cut(s) 550
BtsIMutI CAGTG 1 cut(s) 294
Cac8I GCNNGC 2 cut(s) 73, 144
Cfr10I RCCGGY 1 cut(s) 453
Cfr13I GGNCC 1 cut(s) 309
Csp6I GTAC 2 cut(s) 325, 565
CviAII CATG 3 cut(s) 113, 295, 544
CviQI GTAC 2 cut(s) 325, 565
DdeI CTNAG 2 cut(s) 134, 273
DpnI GATC 1 cut(s) 204
DpnII GATC 1 cut(s) 202
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
Eco24I GRGCYC 1 cut(s) 362
Eco57I CTGAAG 1 cut(s) 516
Eco81I CCTNAGG 1 cut(s) 273
EcoNI CCTNNNNNAGG 1 cut(s) 353
EcoRII CCWGG 4 cut(s) 30, 234, 348, 559
EcoT38I GRGCYC 1 cut(s) 362
FaeI CATG 3 cut(s) 116, 298, 547
FaiI YATR 5 cut(s) 41, 114, 296, 525, 545
FatI CATG 3 cut(s) 112, 294, 543
Fnu4HI GCNGC 1 cut(s) 147
FriOI GRGCYC 1 cut(s) 362
Fsp4HI GCNGC 1 cut(s) 147
FspBI CTAG 3 cut(s) 267, 380, 568
GluI GCNGC 1 cut(s) 147
GsuI CTGGAG 1 cut(s) 275
HaeIII GGCC 3 cut(s) 302, 311, 470
HapII CCGG 2 cut(s) 374, 454
Hin1II CATG 3 cut(s) 116, 298, 547
HindIII AAGCTT 1 cut(s) 87
HinfI GANTC 1 cut(s) 556
HpaII CCGG 2 cut(s) 374, 454
HphI GGTGA 3 cut(s) 94, 128, 496
Hpy166II GTNNAC 1 cut(s) 325
Hpy188I TCNGA 2 cut(s) 103, 440
Hpy188III TCNNGA 5 cut(s) 175, 254, 272, 518, 538
Hpy8I GTNNAC 1 cut(s) 325
HpyAV CCTTC 1 cut(s) 460
HpyCH4III ACNGT 2 cut(s) 289, 428
HpyCH4V TGCA 2 cut(s) 25, 246
HpyF10VI GCNNNNNNNGC 3 cut(s) 308, 317, 411
HpyF3I CTNAG 2 cut(s) 134, 273
Hsp92II CATG 3 cut(s) 116, 298, 547
Kzo9I GATC 1 cut(s) 202
LmnI GCTCC 2 cut(s) 223, 365
Lsp1109I GCAGC 1 cut(s) 133
MaeI CTAG 3 cut(s) 267, 380, 568
MaeIII GTNAC 2 cut(s) 419, 499
MalI GATC 1 cut(s) 204
MboI GATC 1 cut(s) 202
MboII GAAGA 2 cut(s) 106, 395
MhlI GDGCHC 1 cut(s) 362
MluCI AATT 2 cut(s) 16, 259
MnlI CCTC 7 cut(s) 196, 231, 268, 349, 379, 475, 520
MslI CAYNNNNRTG 2 cut(s) 44, 117
MspI CCGG 2 cut(s) 374, 454
MspR9I CCNGG 4 cut(s) 32, 236, 350, 561
MvaI CCWGG 4 cut(s) 32, 236, 350, 561
MwoI GCNNNNNNNGC 3 cut(s) 308, 317, 411
NdeII GATC 1 cut(s) 202
NlaIII CATG 3 cut(s) 116, 298, 547
NlaIV GGNNCC 1 cut(s) 361
NmuCI GTSAC 1 cut(s) 499
PfeI GAWTC 1 cut(s) 556
PflMI CCANNNNNTGG 1 cut(s) 349
PkrI GCNGC 1 cut(s) 148
Psp6I CCWGG 4 cut(s) 30, 234, 348, 559
PspGI CCWGG 4 cut(s) 30, 234, 348, 559
PspN4I GGNNCC 1 cut(s) 361
PspPI GGNCC 1 cut(s) 309
PsrI GAACNNNNNNTAC 2 cut(s) 412, 444
PstI CTGCAG 1 cut(s) 248
RsaI GTAC 2 cut(s) 326, 566
RsaNI GTAC 2 cut(s) 325, 565
RseI CAYNNNNRTG 2 cut(s) 44, 117
SatI GCNGC 1 cut(s) 147
Sau3AI GATC 1 cut(s) 202
Sau96I GGNCC 1 cut(s) 309
ScrFI CCNGG 4 cut(s) 32, 236, 350, 561
SduI GDGCHC 1 cut(s) 362
SfcI CTRYAG 1 cut(s) 244
SfiI GGCCNNNNNGGCC 1 cut(s) 308
SmiMI CAYNNNNRTG 2 cut(s) 44, 117
SpeI ACTAGT 1 cut(s) 266
Sse9I AATT 2 cut(s) 16, 259
SspMI CTAG 3 cut(s) 267, 380, 568
StyD4I CCNGG 4 cut(s) 30, 234, 348, 559
TaaI ACNGT 2 cut(s) 289, 428
TasI AATT 2 cut(s) 16, 259
TfiI GAWTC 1 cut(s) 556
TscAI CASTG 1 cut(s) 294
TseFI GTSAC 1 cut(s) 499
TseI GCWGC 1 cut(s) 146
Tsp45I GTSAC 1 cut(s) 499
TspRI CASTG 1 cut(s) 294
Van91I CCANNNNNTGG 1 cut(s) 349
XagI CCTNNNNNAGG 1 cut(s) 353
XapI RAATTY 1 cut(s) 259
XspI CTAG 3 cut(s) 267, 380, 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.