Rh4CG273200

seed maturation protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0009388
Physical Location & Seq
Reverse (-)
52825851 .. 52828747
2897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG273200.1

Sequence Viewer

Length: 942 bp
ATGGACGTGACACCCAAGCCCGGAGTTCCCTGCCGCGGGATGACCGACACGTGGCTGAAGAAACACACTTTGATCTACACCGGAGCCACCCGACACCCTTTTATCCTCGCCATTCGCGACGGCACCGTCGACATCTCCGCCTTCAAACGGTGGCTGAGCCAGGACTACTTATTTGTGAGAGCTTTTGTGCCGTTTGTGGCGAGTTTGGTGGTGAAAGCTTGGAAGAAGTCTGATGATAGTCATGGTGACGTGGAAGTAATACTGAGTGGGCTTGCTGCTTTGAACGATGAGATTGACTGGTTCAAGAAACAAGCCTCCAAATGGGGTGTTGATCTCTCTGTTGTTGCTCCTCAAAAGCCTACCCAGGATTACTGCAGATTTCTGGAGAATTTGACTAGTCCTGAGGTTGATTACACAGTGGGCATCACGGCCTATTGGGCCATTGAAGCTGTGTACCAAGAGAGCTTTGCCCATTGCCTGGGGGAGGGCTCCAAAACGCCACCGGAACTAGAAGAGGTTTGTCAAAGATGGGGCAATGATGGCTTCGGTAACTACTGTTCTGCTCTCCGAAGCATTGCTGACCGGCGTTTGTTGAAGGCCACAGATGATGAGGTGAGTAAAGCTGAAGTGACATTCCTGCGTGTTCTTGAATATGAGGTTGAGTTCTGGAACATGAGCCGTGGGATTCCTGGGTCTGGCCGACGAGTCAATGTTCATATAAGCCGTCGATTGCTCTTGGAGGCTGAAGAAGCTCCAGTGCCAGCTCCGATCGCTGCAATTTCGGGTCAGCCACATCGGGCGTTCGGCTCCGATCGCTGCAATTTCGGATCTGCTAAGGAACGGCAAAGCCTCCATGGATGGACGCTGCCGTCCAGTCCCCAACGCTTAGCTCCTGGCGAACCGATGTCCAATCCTCATCCGGTGAAGACTGCAGTGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00730 ko01100 map00730 map01100
Pfam Domains
Protein Families
TENA_THI-4

Protein Analysis

313

Amino Acids

34.9

Weight (kDa)

6.46

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TENA_THI-4 PF03070 20 - 227 6.4e-40 TENA/THI-4/PQQC family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 125, 868
AccB1I GGYRCC 1 cut(s) 122
AccB7I CCANNNNNTGG 1 cut(s) 478
AccI GTMKAC 1 cut(s) 129
AccII CGCG 2 cut(s) 36, 117
AciI CCGC 3 cut(s) 34, 36, 138
AclWI GGATC 1 cut(s) 835
AcoI YGGCCR 1 cut(s) 697
AcsI RAATTY 1 cut(s) 388
AcuI CTGAAG 3 cut(s) 77, 645, 765
AcvI CACGTG 1 cut(s) 51
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 9 cut(s) 20, 35, 36, 51, 147, 321, 478, 484, 695
AflIII ACRYGT 1 cut(s) 48
AgsI TTSAA 6 cut(s) 145, 283, 304, 446, 595, 650
AhlI ACTAGT 1 cut(s) 395
AjiI CACGTC 2 cut(s) 7, 250
AjnI CCWGG 5 cut(s) 159, 363, 477, 688, 892
AluBI AGCT 8 cut(s) 182, 218, 449, 465, 623, 752, 764, 890
AluI AGCT 8 cut(s) 182, 218, 449, 465, 623, 752, 764, 890
AlwI GGATC 1 cut(s) 835
AoxI GGCC 4 cut(s) 429, 438, 597, 697
ApeKI GCWGC 4 cut(s) 275, 773, 816, 865
ApoI RAATTY 1 cut(s) 388
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
AspS9I GGNCC 1 cut(s) 438
AsuC2I CCSGG 1 cut(s) 21
AsuHPI GGTGA 4 cut(s) 223, 257, 625, 934
AxyI CCTNAGG 1 cut(s) 402
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 1 cut(s) 491
BbrPI CACGTG 1 cut(s) 51
BbsI GAAGAC 1 cut(s) 932
BbvI GCAGC 4 cut(s) 262, 760, 803, 852
BccI CCATC 3 cut(s) 522, 533, 852
BceAI ACGGC 7 cut(s) 136, 175, 444, 663, 708, 853, 857
BciT130I CCWGG 5 cut(s) 161, 365, 479, 690, 894
BcnI CCSGG 1 cut(s) 21
BcuI ACTAGT 1 cut(s) 395
BfaI CTAG 3 cut(s) 396, 509, 940
BfmI CTRYAG 2 cut(s) 373, 930
BglI GCCNNNNNGGC 1 cut(s) 437
BisI GCNGC 5 cut(s) 34, 276, 774, 817, 866
BlpI GCTNAGC 2 cut(s) 155, 886
BlsI GCNGC 5 cut(s) 35, 277, 775, 818, 867
Bme1390I CCNGG 6 cut(s) 21, 161, 365, 479, 690, 894
BmgBI CACGTC 2 cut(s) 7, 250
BmgT120I GGNCC 1 cut(s) 438
BmiI GGNNCC 4 cut(s) 85, 124, 490, 808
BmrFI CCNGG 6 cut(s) 21, 161, 365, 479, 690, 894
BmsI GCATC 1 cut(s) 432
BpiI GAAGAC 1 cut(s) 932
BpmI CTGGAG 2 cut(s) 404, 738
Bpu10I CCTNAGC 1 cut(s) 834
Bpu1102I GCTNAGC 2 cut(s) 155, 886
BpuMI CCSGG 1 cut(s) 21
BsaAI YACGTR 1 cut(s) 51
BsaJI CCNNGG 6 cut(s) 34, 363, 478, 679, 689, 853
BsaWI WCCGGW 3 cut(s) 80, 502, 919
Bsc4I CCNNNNNNNGG 9 cut(s) 20, 35, 36, 51, 147, 321, 478, 484, 695
Bse118I RCCGGY 1 cut(s) 582
Bse1I ACTGG 3 cut(s) 302, 755, 873
Bse21I CCTNAGG 1 cut(s) 402
Bse3DI GCAATG 3 cut(s) 472, 541, 573
BseBI CCWGG 5 cut(s) 161, 365, 479, 690, 894
BseDI CCNNGG 6 cut(s) 34, 363, 478, 679, 689, 853
BseGI GGATG 3 cut(s) 45, 863, 916
BseLI CCNNNNNNNGG 9 cut(s) 20, 35, 36, 51, 147, 321, 478, 484, 695
BseMI GCAATG 3 cut(s) 472, 541, 573
BseMII CTCAG 3 cut(s) 146, 254, 393
BseNI ACTGG 3 cut(s) 302, 755, 873
BseRI GAGGAG 1 cut(s) 339
BseXI GCAGC 4 cut(s) 262, 760, 803, 852
Bsh1236I CGCG 2 cut(s) 36, 117
Bsh1285I CGRYCG 2 cut(s) 771, 814
BshFI GGCC 4 cut(s) 431, 440, 599, 699
BshNI GGYRCC 1 cut(s) 122
BsiEI CGRYCG 2 cut(s) 771, 814
BsiSI CCGG 5 cut(s) 21, 81, 503, 583, 920
BslFI GGGAC 1 cut(s) 861
BslI CCNNNNNNNGG 9 cut(s) 20, 35, 36, 51, 147, 321, 478, 484, 695
BsmFI GGGAC 1 cut(s) 861
BsnI GGCC 4 cut(s) 431, 440, 599, 699
Bsp1286I GDGCHC 1 cut(s) 491
Bsp143I GATC 5 cut(s) 72, 331, 768, 811, 827
Bsp1720I GCTNAGC 2 cut(s) 155, 886
Bsp19I CCATGG 1 cut(s) 853
Bsp68I TCGCGA 1 cut(s) 117
BspACI CCGC 3 cut(s) 34, 36, 138
BspANI GGCC 4 cut(s) 431, 440, 599, 699
BspCNI CTCAG 3 cut(s) 147, 255, 394
BspFNI CGCG 2 cut(s) 36, 117
BspLI GGNNCC 4 cut(s) 85, 124, 490, 808
BspMAI CTGCAG 2 cut(s) 377, 934
BspPI GGATC 1 cut(s) 835
BspT107I GGYRCC 1 cut(s) 122
BsrDI GCAATG 3 cut(s) 472, 541, 573
BsrFI RCCGGY 1 cut(s) 582
BsrI ACTGG 3 cut(s) 302, 755, 873
BssAI RCCGGY 1 cut(s) 582
BssECI CCNNGG 6 cut(s) 34, 363, 478, 679, 689, 853
BssMI GATC 5 cut(s) 72, 331, 768, 811, 827
BssT1I CCWWGG 1 cut(s) 853
Bst2UI CCWGG 5 cut(s) 161, 365, 479, 690, 894
Bst4CI ACNGT 4 cut(s) 127, 150, 418, 557
Bst6I CTCTTC 1 cut(s) 507
BstBAI YACGTR 1 cut(s) 51
BstC8I GCNNGC 2 cut(s) 273, 762
BstDEI CTNAG 5 cut(s) 155, 263, 402, 834, 886
BstDSI CCRYGG 3 cut(s) 34, 679, 853
BstENI CCTNNNNNAGG 1 cut(s) 482
BstF5I GGATG 3 cut(s) 45, 863, 916
BstFNI CGCG 2 cut(s) 36, 117
BstKTI GATC 5 cut(s) 75, 334, 771, 814, 830
BstMBI GATC 5 cut(s) 72, 331, 768, 811, 827
BstMCI CGRYCG 2 cut(s) 771, 814
BstMWI GCNNNNNNNGC 6 cut(s) 437, 446, 540, 749, 770, 813
BstNI CCWGG 5 cut(s) 161, 365, 479, 690, 894
BstSCI CCNGG 6 cut(s) 19, 159, 363, 477, 688, 892
BstSFI CTRYAG 2 cut(s) 373, 930
BstUI CGCG 2 cut(s) 36, 117
BstV1I GCAGC 4 cut(s) 262, 760, 803, 852
BstV2I GAAGAC 1 cut(s) 932
BstX2I RGATCY 1 cut(s) 827
BstYI RGATCY 1 cut(s) 827
Bsu36I CCTNAGG 1 cut(s) 402
BsuRI GGCC 4 cut(s) 431, 440, 599, 699
BtgI CCRYGG 3 cut(s) 34, 679, 853
BtrI CACGTC 2 cut(s) 7, 250
BtsCI GGATG 3 cut(s) 45, 863, 916
BtsI GCAGTG 1 cut(s) 939
BtsIMutI CAGTG 3 cut(s) 423, 762, 939
BtuMI TCGCGA 1 cut(s) 117
Cac8I GCNNGC 2 cut(s) 273, 762
Cfr10I RCCGGY 1 cut(s) 582
Cfr13I GGNCC 1 cut(s) 438
Cfr42I CCGCGG 1 cut(s) 37
CseI GACGC 1 cut(s) 871
Csp6I GTAC 1 cut(s) 454
CviAII CATG 3 cut(s) 242, 673, 854
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 5 cut(s) 155, 263, 402, 834, 886
DpnI GATC 5 cut(s) 74, 333, 770, 813, 829
DpnII GATC 5 cut(s) 72, 331, 768, 811, 827
DrdI GACNNNNNNGTC 2 cut(s) 125, 868
DseDI GACNNNNNNGTC 2 cut(s) 125, 868
EaeI YGGCCR 1 cut(s) 697
Eam1104I CTCTTC 1 cut(s) 507
EarI CTCTTC 1 cut(s) 507
EciI GGCGGA 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 853
Eco24I GRGCYC 1 cut(s) 491
Eco57I CTGAAG 3 cut(s) 77, 645, 765
Eco72I CACGTG 1 cut(s) 51
Eco81I CCTNAGG 1 cut(s) 402
EcoNI CCTNNNNNAGG 1 cut(s) 482
EcoRII CCWGG 5 cut(s) 159, 363, 477, 688, 892
EcoT14I CCWWGG 1 cut(s) 853
EcoT38I GRGCYC 1 cut(s) 491
ErhI CCWWGG 1 cut(s) 853
FaeI CATG 3 cut(s) 245, 676, 857
FaiI YATR 6 cut(s) 243, 654, 674, 717, 719, 855
FaqI GGGAC 1 cut(s) 861
FatI CATG 3 cut(s) 241, 672, 853
FauI CCCGC 1 cut(s) 29
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 5 cut(s) 34, 276, 774, 817, 866
FokI GGATG 3 cut(s) 52, 870, 903
FriOI GRGCYC 1 cut(s) 491
Fsp4HI GCNGC 5 cut(s) 34, 276, 774, 817, 866
FspBI CTAG 3 cut(s) 396, 509, 940
GluI GCNGC 5 cut(s) 34, 276, 774, 817, 866
GsuI CTGGAG 2 cut(s) 404, 738
HaeIII GGCC 4 cut(s) 431, 440, 599, 699
HapII CCGG 5 cut(s) 21, 81, 503, 583, 920
HgaI GACGC 1 cut(s) 871
Hin1II CATG 3 cut(s) 245, 676, 857
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HindIII AAGCTT 1 cut(s) 216
HinfI GANTC 2 cut(s) 685, 705
HpaII CCGG 5 cut(s) 21, 81, 503, 583, 920
HphI GGTGA 4 cut(s) 223, 257, 625, 934
Hpy166II GTNNAC 3 cut(s) 130, 454, 937
Hpy188I TCNGA 5 cut(s) 232, 569, 768, 811, 827
Hpy188III TCNNGA 6 cut(s) 116, 304, 383, 401, 647, 667
Hpy8I GTNNAC 3 cut(s) 130, 454, 937
Hpy99I CGWCG 4 cut(s) 122, 131, 705, 729
HpyAV CCTTC 2 cut(s) 151, 589
HpyCH4III ACNGT 4 cut(s) 127, 150, 418, 557
HpyCH4IV ACGT 3 cut(s) 6, 50, 249
HpyCH4V TGCA 4 cut(s) 375, 776, 819, 932
HpyF10VI GCNNNNNNNGC 6 cut(s) 437, 446, 540, 749, 770, 813
HpyF3I CTNAG 5 cut(s) 155, 263, 402, 834, 886
HpySE526I ACGT 3 cut(s) 6, 50, 249
Hsp92II CATG 3 cut(s) 245, 676, 857
KspI CCGCGG 1 cut(s) 37
Kzo9I GATC 5 cut(s) 72, 331, 768, 811, 827
LmnI GCTCC 7 cut(s) 83, 352, 494, 757, 769, 812, 895
Lsp1109I GCAGC 4 cut(s) 262, 760, 803, 852
LweI GCATC 1 cut(s) 432
MaeI CTAG 3 cut(s) 396, 509, 940
MaeII ACGT 3 cut(s) 6, 50, 249
MaeIII GTNAC 4 cut(s) 7, 245, 548, 628
MalI GATC 5 cut(s) 74, 333, 770, 813, 829
MboI GATC 5 cut(s) 72, 331, 768, 811, 827
MboII GAAGA 5 cut(s) 70, 235, 524, 758, 937
MflI RGATCY 1 cut(s) 827
MhlI GDGCHC 1 cut(s) 491
MluCI AATT 3 cut(s) 388, 777, 820
MlyI GAGTC 1 cut(s) 714
MspA1I CMGCKG 1 cut(s) 36
MspI CCGG 5 cut(s) 21, 81, 503, 583, 920
MspR9I CCNGG 6 cut(s) 21, 161, 365, 479, 690, 894
MvaI CCWGG 5 cut(s) 161, 365, 479, 690, 894
MvnI CGCG 2 cut(s) 36, 117
MwoI GCNNNNNNNGC 6 cut(s) 437, 446, 540, 749, 770, 813
NciI CCSGG 1 cut(s) 21
NcoI CCATGG 1 cut(s) 853
NdeII GATC 5 cut(s) 72, 331, 768, 811, 827
NlaIII CATG 3 cut(s) 245, 676, 857
NlaIV GGNNCC 4 cut(s) 85, 124, 490, 808
NmuCI GTSAC 3 cut(s) 7, 245, 628
NruI TCGCGA 1 cut(s) 117
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 126
PfeI GAWTC 1 cut(s) 685
PflMI CCANNNNNTGG 1 cut(s) 478
PkrI GCNGC 5 cut(s) 35, 277, 775, 818, 867
Ple19I CGATCG 2 cut(s) 771, 814
PleI GAGTC 1 cut(s) 713
PmaCI CACGTG 1 cut(s) 51
PmlI CACGTG 1 cut(s) 51
PpsI GAGTC 1 cut(s) 713
Ppu21I YACGTR 1 cut(s) 51
Psp6I CCWGG 5 cut(s) 159, 363, 477, 688, 892
PspCI CACGTG 1 cut(s) 51
PspGI CCWGG 5 cut(s) 159, 363, 477, 688, 892
PspN4I GGNNCC 4 cut(s) 85, 124, 490, 808
PspPI GGNCC 1 cut(s) 438
PsrI GAACNNNNNNTAC 2 cut(s) 541, 573
PstI CTGCAG 2 cut(s) 377, 934
PsuI RGATCY 1 cut(s) 827
PvuI CGATCG 2 cut(s) 771, 814
RruI TCGCGA 1 cut(s) 117
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
SacII CCGCGG 1 cut(s) 37
SalI GTCGAC 1 cut(s) 128
SatI GCNGC 5 cut(s) 34, 276, 774, 817, 866
Sau3AI GATC 5 cut(s) 72, 331, 768, 811, 827
Sau96I GGNCC 1 cut(s) 438
SchI GAGTC 1 cut(s) 714
ScrFI CCNGG 6 cut(s) 21, 161, 365, 479, 690, 894
SduI GDGCHC 1 cut(s) 491
SfaNI GCATC 1 cut(s) 432
SfcI CTRYAG 2 cut(s) 373, 930
SfiI GGCCNNNNNGGCC 1 cut(s) 437
Sfr303I CCGCGG 1 cut(s) 37
SgrBI CCGCGG 1 cut(s) 37
SpeI ACTAGT 1 cut(s) 395
Sse9I AATT 3 cut(s) 388, 777, 820
SsiI CCGC 3 cut(s) 34, 36, 138
SspMI CTAG 3 cut(s) 396, 509, 940
StyD4I CCNGG 6 cut(s) 19, 159, 363, 477, 688, 892
StyI CCWWGG 1 cut(s) 853
TaaI ACNGT 4 cut(s) 127, 150, 418, 557
TaiI ACGT 3 cut(s) 9, 53, 252
TaqI TCGA 2 cut(s) 129, 727
TaqII GACCGA 1 cut(s) 59
TasI AATT 3 cut(s) 388, 777, 820
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 1 cut(s) 685
TscAI CASTG 3 cut(s) 423, 762, 939
TseFI GTSAC 3 cut(s) 7, 245, 628
TseI GCWGC 4 cut(s) 275, 773, 816, 865
Tsp45I GTSAC 3 cut(s) 7, 245, 628
TspDTI ATGAA 1 cut(s) 704
TspRI CASTG 3 cut(s) 423, 762, 939
Van91I CCANNNNNTGG 1 cut(s) 478
XagI CCTNNNNNAGG 1 cut(s) 482
XapI RAATTY 1 cut(s) 388
XmiI GTMKAC 1 cut(s) 129
XspI CTAG 3 cut(s) 396, 509, 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.