Rh4DG257600

seed maturation protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0009388
Physical Location & Seq
Reverse (-)
47435501 .. 47435857
357 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG257600.1

Sequence Viewer

Length: 357 bp
ATGATGATCGAGTACTATCTGGAACGGTTTTACAGATTTCTGGAGAATTTGACTAGTCCTGAGGTTGATTACACAGTGGGCATGACGGCCTATTGGGCCATTGAAGCTGTGTACCAAGAGAGTTTTGCCCATTGCCTGGGGGAGGGCTCCAAAACCCCACCGGAACTAGAAGAGGTTTGTCAAAGATGGGGCAATGATGGCTTCGGTAACTACTGTTCTGCTCTCCGAAGCATTGCTGACCGGCGTTTGTTGAAGGCCACAGATGATGAGGTGAGTAAAGCTGAAGTGACATTCCTGTGTGTTCTTGAATATGAGGTTGAGTTCTGGAACATGAGCCGTGGGATTCCTGGGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00730 ko01100 map00730 map01100
Pfam Domains
Protein Families
TENA_THI-4

Protein Analysis

118

Amino Acids

13.71

Weight (kDa)

4.45

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TENA_THI-4 PF03070 16 - 113 8.6e-15 TENA/THI-4/PQQC family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 136
AcsI RAATTY 1 cut(s) 46
AcuI CTGAAG 1 cut(s) 303
AfaI GTAC 3 cut(s) 14, 113, 353
AfiI CCNNNNNNNGG 2 cut(s) 136, 142
AgsI TTSAA 3 cut(s) 104, 253, 308
AhlI ACTAGT 1 cut(s) 53
AjnI CCWGG 2 cut(s) 135, 346
AluBI AGCT 2 cut(s) 107, 281
AluI AGCT 2 cut(s) 107, 281
AoxI GGCC 3 cut(s) 87, 96, 255
ApoI RAATTY 1 cut(s) 46
AspS9I GGNCC 1 cut(s) 96
AsuHPI GGTGA 1 cut(s) 283
AxyI CCTNAGG 1 cut(s) 60
BanII GRGCYC 1 cut(s) 149
BccI CCATC 2 cut(s) 180, 191
BceAI ACGGC 2 cut(s) 102, 321
BciT130I CCWGG 2 cut(s) 137, 348
BcuI ACTAGT 1 cut(s) 53
BfaI CTAG 3 cut(s) 54, 167, 355
BglI GCCNNNNNGGC 1 cut(s) 95
BmcAI AGTACT 1 cut(s) 14
Bme1390I CCNGG 2 cut(s) 137, 348
BmgT120I GGNCC 1 cut(s) 96
BmiI GGNNCC 1 cut(s) 148
BmrFI CCNGG 2 cut(s) 137, 348
BpmI CTGGAG 1 cut(s) 62
BsaJI CCNNGG 3 cut(s) 136, 337, 347
BsaWI WCCGGW 1 cut(s) 160
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 142
Bse118I RCCGGY 1 cut(s) 240
Bse21I CCTNAGG 1 cut(s) 60
Bse3DI GCAATG 3 cut(s) 130, 199, 231
BseBI CCWGG 2 cut(s) 137, 348
BseDI CCNNGG 3 cut(s) 136, 337, 347
BseLI CCNNNNNNNGG 2 cut(s) 136, 142
BseMI GCAATG 3 cut(s) 130, 199, 231
BseMII CTCAG 1 cut(s) 51
BshFI GGCC 3 cut(s) 89, 98, 257
BsiSI CCGG 2 cut(s) 161, 241
BslI CCNNNNNNNGG 2 cut(s) 136, 142
BsnI GGCC 3 cut(s) 89, 98, 257
Bsp1286I GDGCHC 1 cut(s) 149
Bsp143I GATC 1 cut(s) 6
BspANI GGCC 3 cut(s) 89, 98, 257
BspCNI CTCAG 1 cut(s) 52
BspLI GGNNCC 1 cut(s) 148
BsrDI GCAATG 3 cut(s) 130, 199, 231
BsrFI RCCGGY 1 cut(s) 240
BssAI RCCGGY 1 cut(s) 240
BssECI CCNNGG 3 cut(s) 136, 337, 347
BssMI GATC 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 137, 348
Bst4CI ACNGT 3 cut(s) 27, 76, 215
Bst6I CTCTTC 1 cut(s) 165
BstDEI CTNAG 1 cut(s) 60
BstDSI CCRYGG 1 cut(s) 337
BstENI CCTNNNNNAGG 1 cut(s) 140
BstKTI GATC 1 cut(s) 9
BstMBI GATC 1 cut(s) 6
BstMWI GCNNNNNNNGC 3 cut(s) 95, 104, 198
BstNI CCWGG 2 cut(s) 137, 348
BstSCI CCNGG 2 cut(s) 135, 346
Bsu36I CCTNAGG 1 cut(s) 60
BsuRI GGCC 3 cut(s) 89, 98, 257
BtgI CCRYGG 1 cut(s) 337
BtsIMutI CAGTG 1 cut(s) 81
Cfr10I RCCGGY 1 cut(s) 240
Cfr13I GGNCC 1 cut(s) 96
Csp6I GTAC 3 cut(s) 13, 112, 352
CviAII CATG 2 cut(s) 82, 331
CviJI RGCY 8 cut(s) 89, 98, 107, 147, 201, 257, 281, 336
CviKI_1 RGCY 8 cut(s) 89, 98, 107, 147, 201, 257, 281, 336
CviQI GTAC 3 cut(s) 13, 112, 352
DdeI CTNAG 1 cut(s) 60
DpnI GATC 1 cut(s) 8
DpnII GATC 1 cut(s) 6
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
Eco24I GRGCYC 1 cut(s) 149
Eco57I CTGAAG 1 cut(s) 303
Eco81I CCTNAGG 1 cut(s) 60
EcoNI CCTNNNNNAGG 1 cut(s) 140
EcoRII CCWGG 2 cut(s) 135, 346
EcoT38I GRGCYC 1 cut(s) 149
FaeI CATG 2 cut(s) 85, 334
FaiI YATR 3 cut(s) 83, 312, 332
FatI CATG 2 cut(s) 81, 330
FriOI GRGCYC 1 cut(s) 149
FspBI CTAG 3 cut(s) 54, 167, 355
GsuI CTGGAG 1 cut(s) 62
HaeIII GGCC 3 cut(s) 89, 98, 257
HapII CCGG 2 cut(s) 161, 241
Hin1II CATG 2 cut(s) 85, 334
HinfI GANTC 1 cut(s) 343
HpaII CCGG 2 cut(s) 161, 241
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 1 cut(s) 112
Hpy188I TCNGA 1 cut(s) 227
Hpy188III TCNNGA 5 cut(s) 20, 41, 59, 305, 325
Hpy8I GTNNAC 1 cut(s) 112
HpyAV CCTTC 1 cut(s) 247
HpyCH4III ACNGT 3 cut(s) 27, 76, 215
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 104, 198
HpyF3I CTNAG 1 cut(s) 60
Hsp92II CATG 2 cut(s) 85, 334
Kzo9I GATC 1 cut(s) 6
LmnI GCTCC 1 cut(s) 152
MaeI CTAG 3 cut(s) 54, 167, 355
MaeIII GTNAC 2 cut(s) 206, 286
MalI GATC 1 cut(s) 8
MboI GATC 1 cut(s) 6
MboII GAAGA 1 cut(s) 182
MhlI GDGCHC 1 cut(s) 149
MluCI AATT 1 cut(s) 46
MnlI CCTC 5 cut(s) 55, 136, 166, 262, 307
MslI CAYNNNNRTG 1 cut(s) 295
MspI CCGG 2 cut(s) 161, 241
MspR9I CCNGG 2 cut(s) 137, 348
MvaI CCWGG 2 cut(s) 137, 348
MwoI GCNNNNNNNGC 3 cut(s) 95, 104, 198
NdeII GATC 1 cut(s) 6
NlaIII CATG 2 cut(s) 85, 334
NlaIV GGNNCC 1 cut(s) 148
NmuCI GTSAC 1 cut(s) 286
PfeI GAWTC 1 cut(s) 343
PflMI CCANNNNNTGG 1 cut(s) 136
Psp6I CCWGG 2 cut(s) 135, 346
PspGI CCWGG 2 cut(s) 135, 346
PspN4I GGNNCC 1 cut(s) 148
PspPI GGNCC 1 cut(s) 96
PsrI GAACNNNNNNTAC 2 cut(s) 199, 231
RsaI GTAC 3 cut(s) 14, 113, 353
RsaNI GTAC 3 cut(s) 13, 112, 352
RseI CAYNNNNRTG 1 cut(s) 295
Sau3AI GATC 1 cut(s) 6
Sau96I GGNCC 1 cut(s) 96
ScaI AGTACT 1 cut(s) 14
ScrFI CCNGG 2 cut(s) 137, 348
SduI GDGCHC 1 cut(s) 149
SetI ASST 6 cut(s) 66, 109, 177, 273, 283, 318
SfiI GGCCNNNNNGGCC 1 cut(s) 95
SmiMI CAYNNNNRTG 1 cut(s) 295
SpeI ACTAGT 1 cut(s) 53
Sse9I AATT 1 cut(s) 46
SspMI CTAG 3 cut(s) 54, 167, 355
StyD4I CCNGG 2 cut(s) 135, 346
TaaI ACNGT 3 cut(s) 27, 76, 215
TaqI TCGA 1 cut(s) 9
TasI AATT 1 cut(s) 46
TatI WGTACW 1 cut(s) 12
TfiI GAWTC 1 cut(s) 343
TscAI CASTG 1 cut(s) 81
TseFI GTSAC 1 cut(s) 286
Tsp45I GTSAC 1 cut(s) 286
TspRI CASTG 1 cut(s) 81
Van91I CCANNNNNTGG 1 cut(s) 136
XagI CCTNNNNNAGG 1 cut(s) 140
XapI RAATTY 1 cut(s) 46
XspI CTAG 3 cut(s) 54, 167, 355
ZrmI AGTACT 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.