Rw4G024020

seed maturation protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009388
Physical Location & Seq
Reverse (-)
49920583 .. 49921982
1400 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G024020.1

Sequence Viewer

Length: 699 bp
ATGGACTTGACACCCAGGCCCGGAGTTCCCTGCCGCGGGATGACCGACACGTGGCTGAAGAAACACGCTTTGATCTACACCGGAGCCACCCGACACCCTTTTATCCTCGCCATTCGCGACGGCACCGTCGACATCTCCGCCTTCAAACGGTGGCTGAGCCAGGACTACATATTTGTGAGAGCTTTTGTGCCGTTTGTGGCGAGCTTGGTGGTGAAAGCTTGGAGGAAGTCTGATGATAGTCATGGTGATGTGGAAGTGATACTGAGTGGGCTTGCTGCTTTGAACGATGAGATTGACTGGTTCAAGAAACAAGCCTCCAAATGGGGTGTTGATCTCTCTGTTGTTGCTCCTCAAAAGCCTACCCAGGATTACTGCAGATTTCTGGAGAATTTGACTAGTCCTGAGGTTGATTACACAGTGGGCATGACGGCCTATTGGGCCATTGAAGCTGTGTACCAAGAGAGCTTTGCCCATTGCCTGGGGGAGGGCTCCAAAACCCCACCGGAACTAGAAGAGGTATGTCAAAGATGGGGCAATGATGGCTTCGGTAACTACTGTTCTGCTCTCCGAAGCATTGCTGACCGGCGTTTGTTGAAGGCCACAGATGATGAGGTGAGTAAAGCTGAAGTGCCATTCCTGCGTGTTCTTGAATATGAGGTTGAGTTCTGGAACATGAGCCGTGGGATTCCTGGGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00730 ko01100 map00730 map01100
Pfam Domains
Protein Families
TENA_THI-4

Protein Analysis

232

Amino Acids

26.3

Weight (kDa)

5.31

Isoelectric Point (pI)

35.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TENA_THI-4 PF03070 20 - 227 2.1e-39 TENA/THI-4/PQQC family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 125
AccB1I GGYRCC 1 cut(s) 122
AccB7I CCANNNNNTGG 1 cut(s) 478
AccI GTMKAC 1 cut(s) 129
AccII CGCG 2 cut(s) 36, 117
AciI CCGC 3 cut(s) 34, 36, 138
AcsI RAATTY 1 cut(s) 388
AcuI CTGAAG 2 cut(s) 77, 645
AcvI CACGTG 1 cut(s) 51
AfaI GTAC 2 cut(s) 455, 695
AfiI CCNNNNNNNGG 8 cut(s) 20, 35, 36, 51, 147, 321, 478, 484
AflIII ACRYGT 1 cut(s) 48
AgsI TTSAA 6 cut(s) 145, 283, 304, 446, 595, 650
AhlI ACTAGT 1 cut(s) 395
AjnI CCWGG 5 cut(s) 14, 159, 363, 477, 688
AluBI AGCT 6 cut(s) 182, 204, 218, 449, 465, 623
AluI AGCT 6 cut(s) 182, 204, 218, 449, 465, 623
AoxI GGCC 4 cut(s) 17, 429, 438, 597
ApeKI GCWGC 1 cut(s) 275
ApoI RAATTY 1 cut(s) 388
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
AspS9I GGNCC 2 cut(s) 18, 438
AsuC2I CCSGG 1 cut(s) 21
AsuHPI GGTGA 3 cut(s) 223, 257, 625
AxyI CCTNAGG 1 cut(s) 402
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 1 cut(s) 491
BbrPI CACGTG 1 cut(s) 51
BbvI GCAGC 1 cut(s) 262
BccI CCATC 2 cut(s) 522, 533
BceAI ACGGC 4 cut(s) 136, 175, 444, 663
BciT130I CCWGG 5 cut(s) 16, 161, 365, 479, 690
BcnI CCSGG 1 cut(s) 21
BcuI ACTAGT 1 cut(s) 395
BfaI CTAG 3 cut(s) 396, 509, 697
BfmI CTRYAG 1 cut(s) 373
BglI GCCNNNNNGGC 1 cut(s) 437
BisI GCNGC 2 cut(s) 34, 276
BlpI GCTNAGC 1 cut(s) 155
BlsI GCNGC 2 cut(s) 35, 277
Bme1390I CCNGG 6 cut(s) 16, 21, 161, 365, 479, 690
BmgT120I GGNCC 2 cut(s) 18, 438
BmiI GGNNCC 3 cut(s) 85, 124, 490
BmrFI CCNGG 6 cut(s) 16, 21, 161, 365, 479, 690
BpmI CTGGAG 1 cut(s) 404
Bpu1102I GCTNAGC 1 cut(s) 155
BpuMI CCSGG 1 cut(s) 21
BsaAI YACGTR 1 cut(s) 51
BsaJI CCNNGG 6 cut(s) 14, 34, 363, 478, 679, 689
BsaWI WCCGGW 2 cut(s) 80, 502
Bsc4I CCNNNNNNNGG 8 cut(s) 20, 35, 36, 51, 147, 321, 478, 484
Bse118I RCCGGY 1 cut(s) 582
Bse1I ACTGG 1 cut(s) 302
Bse21I CCTNAGG 1 cut(s) 402
Bse3DI GCAATG 3 cut(s) 472, 541, 573
BseBI CCWGG 5 cut(s) 16, 161, 365, 479, 690
BseDI CCNNGG 6 cut(s) 14, 34, 363, 478, 679, 689
BseGI GGATG 1 cut(s) 45
BseLI CCNNNNNNNGG 8 cut(s) 20, 35, 36, 51, 147, 321, 478, 484
BseMI GCAATG 3 cut(s) 472, 541, 573
BseMII CTCAG 3 cut(s) 146, 254, 393
BseNI ACTGG 1 cut(s) 302
BseRI GAGGAG 1 cut(s) 339
BseXI GCAGC 1 cut(s) 262
Bsh1236I CGCG 2 cut(s) 36, 117
BshFI GGCC 4 cut(s) 19, 431, 440, 599
BshNI GGYRCC 1 cut(s) 122
BsiSI CCGG 4 cut(s) 21, 81, 503, 583
BslI CCNNNNNNNGG 8 cut(s) 20, 35, 36, 51, 147, 321, 478, 484
BsnI GGCC 4 cut(s) 19, 431, 440, 599
Bsp1286I GDGCHC 1 cut(s) 491
Bsp143I GATC 2 cut(s) 72, 331
Bsp1720I GCTNAGC 1 cut(s) 155
Bsp68I TCGCGA 1 cut(s) 117
BspACI CCGC 3 cut(s) 34, 36, 138
BspANI GGCC 4 cut(s) 19, 431, 440, 599
BspCNI CTCAG 3 cut(s) 147, 255, 394
BspFNI CGCG 2 cut(s) 36, 117
BspLI GGNNCC 3 cut(s) 85, 124, 490
BspMAI CTGCAG 1 cut(s) 377
BspT107I GGYRCC 1 cut(s) 122
BsrDI GCAATG 3 cut(s) 472, 541, 573
BsrFI RCCGGY 1 cut(s) 582
BsrI ACTGG 1 cut(s) 302
BssAI RCCGGY 1 cut(s) 582
BssECI CCNNGG 6 cut(s) 14, 34, 363, 478, 679, 689
BssMI GATC 2 cut(s) 72, 331
Bst2UI CCWGG 5 cut(s) 16, 161, 365, 479, 690
Bst4CI ACNGT 4 cut(s) 127, 150, 418, 557
Bst6I CTCTTC 1 cut(s) 507
BstBAI YACGTR 1 cut(s) 51
BstC8I GCNNGC 2 cut(s) 202, 273
BstDEI CTNAG 3 cut(s) 155, 263, 402
BstDSI CCRYGG 2 cut(s) 34, 679
BstENI CCTNNNNNAGG 1 cut(s) 482
BstF5I GGATG 1 cut(s) 45
BstFNI CGCG 2 cut(s) 36, 117
BstKTI GATC 2 cut(s) 75, 334
BstMBI GATC 2 cut(s) 72, 331
BstMWI GCNNNNNNNGC 4 cut(s) 437, 446, 540, 637
BstNI CCWGG 5 cut(s) 16, 161, 365, 479, 690
BstSCI CCNGG 6 cut(s) 14, 19, 159, 363, 477, 688
BstSFI CTRYAG 1 cut(s) 373
BstUI CGCG 2 cut(s) 36, 117
BstV1I GCAGC 1 cut(s) 262
Bsu36I CCTNAGG 1 cut(s) 402
BsuRI GGCC 4 cut(s) 19, 431, 440, 599
BtgI CCRYGG 2 cut(s) 34, 679
BtsCI GGATG 1 cut(s) 45
BtsIMutI CAGTG 1 cut(s) 423
BtuMI TCGCGA 1 cut(s) 117
Cac8I GCNNGC 2 cut(s) 202, 273
Cfr10I RCCGGY 1 cut(s) 582
Cfr13I GGNCC 2 cut(s) 18, 438
Cfr42I CCGCGG 1 cut(s) 37
Csp6I GTAC 2 cut(s) 454, 694
CviAII CATG 3 cut(s) 242, 424, 673
CviQI GTAC 2 cut(s) 454, 694
DdeI CTNAG 3 cut(s) 155, 263, 402
DpnI GATC 2 cut(s) 74, 333
DpnII GATC 2 cut(s) 72, 331
DrdI GACNNNNNNGTC 1 cut(s) 125
DseDI GACNNNNNNGTC 1 cut(s) 125
Eam1104I CTCTTC 1 cut(s) 507
EarI CTCTTC 1 cut(s) 507
EciI GGCGGA 1 cut(s) 127
Eco24I GRGCYC 1 cut(s) 491
Eco57I CTGAAG 2 cut(s) 77, 645
Eco72I CACGTG 1 cut(s) 51
Eco81I CCTNAGG 1 cut(s) 402
EcoNI CCTNNNNNAGG 1 cut(s) 482
EcoRII CCWGG 5 cut(s) 14, 159, 363, 477, 688
EcoT38I GRGCYC 1 cut(s) 491
FaeI CATG 3 cut(s) 245, 427, 676
FaiI YATR 6 cut(s) 170, 243, 425, 520, 654, 674
FatI CATG 3 cut(s) 241, 423, 672
FauI CCCGC 1 cut(s) 29
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 2 cut(s) 34, 276
FokI GGATG 1 cut(s) 52
FriOI GRGCYC 1 cut(s) 491
Fsp4HI GCNGC 2 cut(s) 34, 276
FspBI CTAG 3 cut(s) 396, 509, 697
GluI GCNGC 2 cut(s) 34, 276
GsuI CTGGAG 1 cut(s) 404
HaeIII GGCC 4 cut(s) 19, 431, 440, 599
HapII CCGG 4 cut(s) 21, 81, 503, 583
Hin1II CATG 3 cut(s) 245, 427, 676
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HindIII AAGCTT 1 cut(s) 216
HinfI GANTC 1 cut(s) 685
HpaII CCGG 4 cut(s) 21, 81, 503, 583
HphI GGTGA 3 cut(s) 223, 257, 625
Hpy166II GTNNAC 2 cut(s) 130, 454
Hpy188I TCNGA 2 cut(s) 232, 569
Hpy188III TCNNGA 6 cut(s) 116, 304, 383, 401, 647, 667
Hpy8I GTNNAC 2 cut(s) 130, 454
Hpy99I CGWCG 2 cut(s) 122, 131
HpyAV CCTTC 2 cut(s) 151, 589
HpyCH4III ACNGT 4 cut(s) 127, 150, 418, 557
HpyCH4IV ACGT 1 cut(s) 50
HpyCH4V TGCA 1 cut(s) 375
HpyF10VI GCNNNNNNNGC 4 cut(s) 437, 446, 540, 637
HpyF3I CTNAG 3 cut(s) 155, 263, 402
HpySE526I ACGT 1 cut(s) 50
Hsp92II CATG 3 cut(s) 245, 427, 676
KspI CCGCGG 1 cut(s) 37
Kzo9I GATC 2 cut(s) 72, 331
LmnI GCTCC 3 cut(s) 83, 352, 494
Lsp1109I GCAGC 1 cut(s) 262
MaeI CTAG 3 cut(s) 396, 509, 697
MaeII ACGT 1 cut(s) 50
MaeIII GTNAC 1 cut(s) 548
MalI GATC 2 cut(s) 74, 333
MboI GATC 2 cut(s) 72, 331
MboII GAAGA 2 cut(s) 70, 524
MhlI GDGCHC 1 cut(s) 491
MluCI AATT 1 cut(s) 388
MnlI CCTC 9 cut(s) 116, 216, 325, 360, 397, 478, 508, 604, 649
MslI CAYNNNNRTG 2 cut(s) 173, 246
MspA1I CMGCKG 1 cut(s) 36
MspI CCGG 4 cut(s) 21, 81, 503, 583
MspR9I CCNGG 6 cut(s) 16, 21, 161, 365, 479, 690
MvaI CCWGG 5 cut(s) 16, 161, 365, 479, 690
MvnI CGCG 2 cut(s) 36, 117
MwoI GCNNNNNNNGC 4 cut(s) 437, 446, 540, 637
NciI CCSGG 1 cut(s) 21
NdeII GATC 2 cut(s) 72, 331
NlaIII CATG 3 cut(s) 245, 427, 676
NlaIV GGNNCC 3 cut(s) 85, 124, 490
NruI TCGCGA 1 cut(s) 117
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 126
PfeI GAWTC 1 cut(s) 685
PflMI CCANNNNNTGG 1 cut(s) 478
PkrI GCNGC 2 cut(s) 35, 277
PmaCI CACGTG 1 cut(s) 51
PmlI CACGTG 1 cut(s) 51
Ppu21I YACGTR 1 cut(s) 51
Psp6I CCWGG 5 cut(s) 14, 159, 363, 477, 688
PspCI CACGTG 1 cut(s) 51
PspGI CCWGG 5 cut(s) 14, 159, 363, 477, 688
PspN4I GGNNCC 3 cut(s) 85, 124, 490
PspPI GGNCC 2 cut(s) 18, 438
PsrI GAACNNNNNNTAC 2 cut(s) 541, 573
PstI CTGCAG 1 cut(s) 377
RruI TCGCGA 1 cut(s) 117
RsaI GTAC 2 cut(s) 455, 695
RsaNI GTAC 2 cut(s) 454, 694
RseI CAYNNNNRTG 2 cut(s) 173, 246
SacII CCGCGG 1 cut(s) 37
SalI GTCGAC 1 cut(s) 128
SatI GCNGC 2 cut(s) 34, 276
Sau3AI GATC 2 cut(s) 72, 331
Sau96I GGNCC 2 cut(s) 18, 438
ScrFI CCNGG 6 cut(s) 16, 21, 161, 365, 479, 690
SduI GDGCHC 1 cut(s) 491
SfcI CTRYAG 1 cut(s) 373
SfiI GGCCNNNNNGGCC 1 cut(s) 437
Sfr303I CCGCGG 1 cut(s) 37
SgrBI CCGCGG 1 cut(s) 37
SmiMI CAYNNNNRTG 2 cut(s) 173, 246
SpeI ACTAGT 1 cut(s) 395
Sse9I AATT 1 cut(s) 388
SsiI CCGC 3 cut(s) 34, 36, 138
SspMI CTAG 3 cut(s) 396, 509, 697
StyD4I CCNGG 6 cut(s) 14, 19, 159, 363, 477, 688
TaaI ACNGT 4 cut(s) 127, 150, 418, 557
TaiI ACGT 1 cut(s) 53
TaqI TCGA 1 cut(s) 129
TaqII GACCGA 1 cut(s) 59
TasI AATT 1 cut(s) 388
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 1 cut(s) 685
TscAI CASTG 1 cut(s) 423
TseI GCWGC 1 cut(s) 275
TspRI CASTG 1 cut(s) 423
Van91I CCANNNNNTGG 1 cut(s) 478
XagI CCTNNNNNAGG 1 cut(s) 482
XapI RAATTY 1 cut(s) 388
XmiI GTMKAC 1 cut(s) 129
XspI CTAG 3 cut(s) 396, 509, 697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.