RLG00000007423

seed maturation protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0009388
Physical Location & Seq
Forward (+)
16633543 .. 16634004
462 bp
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UTR
Exon/CDS
Intron
RLM00000007423

Sequence Viewer

Length: 381 bp
ATGGACGCGACACCCAAGCCCGGAGTTCCCTACCGCGGGATGACCGACACGTGGCTGAAGAAGCACACTTTCATCTACACCGGAGCCACCCGACACCCTTTTATCCCCGCCATTCGCGACGGCACCGTCGACATCTCCGCCTTCAAACGGTGGCTGAGCCAGGACTACATATTTGTGAGAGCTTTTGTGCCGTTTGTGGCGAGTTTGGTGGTGAAAGCTTGGAAGAAGTCTGATGATAGTCATGGTGATGTGGAAGTGATACTGAATGGGCTTGCTGCTTTGAACGATGAGATTGACTGGTTCAAGAAACAAGCCTCCAAATGGGGTGTTGATCTCTCTCTTGTTGCTCCTCAAAAGCCTACCCAGGATTACTGCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00730 ko01100 map00730 map01100
Pfam Domains
Protein Families
TENA_THI-4

Protein Analysis

127

Amino Acids

14.3

Weight (kDa)

9.04

Isoelectric Point (pI)

24.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TENA_THI-4 PF03070 20 - 125 7.5e-19 TENA/THI-4/PQQC family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 125
AccB1I GGYRCC 1 cut(s) 122
AccI GTMKAC 1 cut(s) 129
AccII CGCG 3 cut(s) 8, 36, 117
AciI CCGC 4 cut(s) 34, 36, 108, 138
AcuI CTGAAG 1 cut(s) 77
AcvI CACGTG 1 cut(s) 51
AfiI CCNNNNNNNGG 6 cut(s) 20, 35, 36, 51, 147, 321
AflIII ACRYGT 1 cut(s) 48
AgsI TTSAA 3 cut(s) 145, 283, 304
AjnI CCWGG 2 cut(s) 159, 363
AluBI AGCT 2 cut(s) 182, 218
AluI AGCT 2 cut(s) 182, 218
ApeKI GCWGC 1 cut(s) 275
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
AsuC2I CCSGG 1 cut(s) 21
AsuHPI GGTGA 2 cut(s) 223, 257
BanI GGYRCC 1 cut(s) 122
BbrPI CACGTG 1 cut(s) 51
BbvI GCAGC 1 cut(s) 262
BceAI ACGGC 2 cut(s) 136, 175
BciT130I CCWGG 2 cut(s) 161, 365
BcnI CCSGG 1 cut(s) 21
BisI GCNGC 1 cut(s) 276
BlpI GCTNAGC 1 cut(s) 155
BlsI GCNGC 1 cut(s) 277
Bme1390I CCNGG 3 cut(s) 21, 161, 365
BmiI GGNNCC 2 cut(s) 85, 124
BmrFI CCNGG 3 cut(s) 21, 161, 365
Bpu1102I GCTNAGC 1 cut(s) 155
BpuMI CCSGG 1 cut(s) 21
BsaAI YACGTR 1 cut(s) 51
BsaJI CCNNGG 2 cut(s) 34, 363
BsaWI WCCGGW 1 cut(s) 80
Bsc4I CCNNNNNNNGG 6 cut(s) 20, 35, 36, 51, 147, 321
Bse118I RCCGGY 1 cut(s) 375
Bse1I ACTGG 1 cut(s) 302
BseBI CCWGG 2 cut(s) 161, 365
BseDI CCNNGG 2 cut(s) 34, 363
BseGI GGATG 1 cut(s) 45
BseLI CCNNNNNNNGG 6 cut(s) 20, 35, 36, 51, 147, 321
BseMII CTCAG 1 cut(s) 146
BseNI ACTGG 1 cut(s) 302
BseRI GAGGAG 1 cut(s) 339
BseXI GCAGC 1 cut(s) 262
Bsh1236I CGCG 3 cut(s) 8, 36, 117
BshNI GGYRCC 1 cut(s) 122
BsiSI CCGG 3 cut(s) 21, 81, 376
BslI CCNNNNNNNGG 6 cut(s) 20, 35, 36, 51, 147, 321
Bsp143I GATC 1 cut(s) 331
Bsp1720I GCTNAGC 1 cut(s) 155
Bsp68I TCGCGA 1 cut(s) 117
BspACI CCGC 4 cut(s) 34, 36, 108, 138
BspCNI CTCAG 1 cut(s) 147
BspFNI CGCG 3 cut(s) 8, 36, 117
BspLI GGNNCC 2 cut(s) 85, 124
BspT107I GGYRCC 1 cut(s) 122
BsrFI RCCGGY 1 cut(s) 375
BsrI ACTGG 1 cut(s) 302
BssAI RCCGGY 1 cut(s) 375
BssECI CCNNGG 2 cut(s) 34, 363
BssMI GATC 1 cut(s) 331
Bst2UI CCWGG 2 cut(s) 161, 365
Bst4CI ACNGT 2 cut(s) 127, 150
BstBAI YACGTR 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 273
BstDEI CTNAG 1 cut(s) 155
BstDSI CCRYGG 1 cut(s) 34
BstF5I GGATG 1 cut(s) 45
BstFNI CGCG 3 cut(s) 8, 36, 117
BstKTI GATC 1 cut(s) 334
BstMBI GATC 1 cut(s) 331
BstMWI GCNNNNNNNGC 1 cut(s) 61
BstNI CCWGG 2 cut(s) 161, 365
BstSCI CCNGG 3 cut(s) 19, 159, 363
BstUI CGCG 3 cut(s) 8, 36, 117
BstV1I GCAGC 1 cut(s) 262
BtgI CCRYGG 1 cut(s) 34
BtsCI GGATG 1 cut(s) 45
BtuMI TCGCGA 1 cut(s) 117
Cac8I GCNNGC 1 cut(s) 273
Cfr10I RCCGGY 1 cut(s) 375
Cfr42I CCGCGG 1 cut(s) 37
CseI GACGC 1 cut(s) 14
CviAII CATG 1 cut(s) 242
DdeI CTNAG 1 cut(s) 155
DpnI GATC 1 cut(s) 333
DpnII GATC 1 cut(s) 331
DrdI GACNNNNNNGTC 1 cut(s) 125
DseDI GACNNNNNNGTC 1 cut(s) 125
EciI GGCGGA 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 77
Eco72I CACGTG 1 cut(s) 51
EcoRII CCWGG 2 cut(s) 159, 363
FaeI CATG 1 cut(s) 245
FaiI YATR 2 cut(s) 170, 243
FatI CATG 1 cut(s) 241
FauI CCCGC 2 cut(s) 29, 115
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 1 cut(s) 276
FokI GGATG 1 cut(s) 52
Fsp4HI GCNGC 1 cut(s) 276
GluI GCNGC 1 cut(s) 276
HapII CCGG 3 cut(s) 21, 81, 376
HgaI GACGC 1 cut(s) 14
Hin1II CATG 1 cut(s) 245
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HindIII AAGCTT 1 cut(s) 216
HpaII CCGG 3 cut(s) 21, 81, 376
HphI GGTGA 2 cut(s) 223, 257
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 1 cut(s) 232
Hpy188III TCNNGA 2 cut(s) 116, 304
Hpy8I GTNNAC 1 cut(s) 130
Hpy99I CGWCG 2 cut(s) 122, 131
HpyAV CCTTC 1 cut(s) 151
HpyCH4III ACNGT 2 cut(s) 127, 150
HpyCH4IV ACGT 1 cut(s) 50
HpyF10VI GCNNNNNNNGC 1 cut(s) 61
HpyF3I CTNAG 1 cut(s) 155
HpySE526I ACGT 1 cut(s) 50
Hsp92II CATG 1 cut(s) 245
KspI CCGCGG 1 cut(s) 37
Kzo9I GATC 1 cut(s) 331
LmnI GCTCC 2 cut(s) 83, 352
LpnPI CCDG 7 cut(s) 34, 94, 146, 173, 283, 350, 377
Lsp1109I GCAGC 1 cut(s) 262
MaeII ACGT 1 cut(s) 50
MalI GATC 1 cut(s) 333
MboI GATC 1 cut(s) 331
MboII GAAGA 2 cut(s) 70, 235
MnlI CCTC 2 cut(s) 325, 360
MslI CAYNNNNRTG 2 cut(s) 173, 246
MspA1I CMGCKG 1 cut(s) 36
MspI CCGG 3 cut(s) 21, 81, 376
MspR9I CCNGG 3 cut(s) 21, 161, 365
MvaI CCWGG 2 cut(s) 161, 365
MvnI CGCG 3 cut(s) 8, 36, 117
MwoI GCNNNNNNNGC 1 cut(s) 61
NciI CCSGG 1 cut(s) 21
NdeII GATC 1 cut(s) 331
NlaIII CATG 1 cut(s) 245
NlaIV GGNNCC 2 cut(s) 85, 124
NruI TCGCGA 1 cut(s) 117
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PkrI GCNGC 1 cut(s) 277
PmaCI CACGTG 1 cut(s) 51
PmlI CACGTG 1 cut(s) 51
Ppu21I YACGTR 1 cut(s) 51
Psp6I CCWGG 2 cut(s) 159, 363
PspCI CACGTG 1 cut(s) 51
PspGI CCWGG 2 cut(s) 159, 363
PspN4I GGNNCC 2 cut(s) 85, 124
RruI TCGCGA 1 cut(s) 117
RseI CAYNNNNRTG 2 cut(s) 173, 246
SacII CCGCGG 1 cut(s) 37
SalI GTCGAC 1 cut(s) 128
SatI GCNGC 1 cut(s) 276
Sau3AI GATC 1 cut(s) 331
ScrFI CCNGG 3 cut(s) 21, 161, 365
SetI ASST 3 cut(s) 53, 184, 220
Sfr303I CCGCGG 1 cut(s) 37
SgrBI CCGCGG 1 cut(s) 37
SmiMI CAYNNNNRTG 2 cut(s) 173, 246
SsiI CCGC 4 cut(s) 34, 36, 108, 138
StyD4I CCNGG 3 cut(s) 19, 159, 363
TaaI ACNGT 2 cut(s) 127, 150
TaiI ACGT 1 cut(s) 53
TaqI TCGA 1 cut(s) 129
TaqII GACCGA 1 cut(s) 59
TseI GCWGC 1 cut(s) 275
TspDTI ATGAA 1 cut(s) 61
XmiI GTMKAC 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.