MD16G1155500.v1.1

seed maturation protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009388
Physical Location & Seq
Forward (+)
12325677 .. 12328127
2451 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1155500.v1.1.491

Sequence Viewer

Length: 729 bp
ATGGATGGGAAGCCGAAAGCTGGAGTGTCGATGACCAGCACGTGGCTGAGGAAGCACCGTCTGATCTACGCTGGAGCCACCCGACACCCCTTCATCCTCAGCATTCGCGACGGCACCGTCGATCTTTCCGCCTTCAAACGCTGGCTGGGCCAGGACTACATATTTGTGAGAGCATTTGTACCATTTGTGGCGAGTGTGCTGATAAAAGCTTGGAAGAATTCCGATCACGAAAGCGGCGATATCGAATTGGTACTGAGTGGGGTGGCTGCTCTGAACGATGAGATTGAATGGTTCAAGCAAGAGGCTTCAAAGTGGGGTGTTGAGCTATCTGGTGTTGTTCCTGAAAAAACAACCCGGGATTACTGCAGATTTCTGGAGGATCTAATGAGCCCGGATGTTGATTACGCTGTGGCCATGACAGCCTTTTGGGCCATCGAAGCTGTATACCAAGAGAGCTTTGCCCATTGCCTGGAAGAAGGGTCCAAAACCCCACCAGAACTGCAAGAGGCTTGCCAAAGGTGGGGCAATGATGGCTTCGGCCAGTACTGCTCCTCTCTCCGAAACATTGCTGACCGAGAGTTGGAGAAGTCCGCCTCAGACGGTGGGCCTTTAGTGAAGGTTTCAGAAGATATGGTCACCAAGGCTGAAGTAGAGTTCCAGCGTGTACTCGAGTATGAGGTTCAGTTTTGGAACATGAGCCATGGGAGTCCTGGACGCTCTGCTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00730 ko01100 map00730 map01100
Pfam Domains
Protein Families
TENA_THI-4

Protein Analysis

243

Amino Acids

27.24

Weight (kDa)

5.06

Isoelectric Point (pI)

49.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TENA_THI-4 PF03070 17 - 233 1.4e-39 TENA/THI-4/PQQC family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 116
AccB1I GGYRCC 1 cut(s) 113
AccB7I CCANNNNNTGG 2 cut(s) 42, 469
AccI GTMKAC 1 cut(s) 444
AccII CGCG 1 cut(s) 108
AciI CCGC 3 cut(s) 129, 234, 591
AclWI GGATC 1 cut(s) 387
AcoI YGGCCR 2 cut(s) 411, 538
AcsI RAATTY 1 cut(s) 217
AcuI CTGAAG 1 cut(s) 666
AcvI CACGTG 1 cut(s) 42
AfaI GTAC 4 cut(s) 180, 252, 545, 666
AfiI CCNNNNNNNGG 5 cut(s) 20, 42, 469, 520, 580
AgsI TTSAA 4 cut(s) 136, 287, 295, 309
AjnI CCWGG 3 cut(s) 150, 468, 709
AluBI AGCT 5 cut(s) 20, 209, 325, 440, 456
AluI AGCT 5 cut(s) 20, 209, 325, 440, 456
AlwI GGATC 1 cut(s) 387
Ama87I CYCGRG 2 cut(s) 354, 668
AoxI GGCC 5 cut(s) 148, 411, 429, 538, 605
ApeKI GCWGC 1 cut(s) 266
ApoI RAATTY 1 cut(s) 217
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
AspS9I GGNCC 4 cut(s) 148, 429, 480, 605
AsuC2I CCSGG 3 cut(s) 355, 356, 392
AsuHPI GGTGA 1 cut(s) 628
AvaI CYCGRG 2 cut(s) 354, 668
AvaII GGWCC 1 cut(s) 480
BalI TGGCCA 1 cut(s) 413
BanI GGYRCC 1 cut(s) 113
BanII GRGCYC 1 cut(s) 392
BbrPI CACGTG 1 cut(s) 42
BbvCI CCTCAGC 2 cut(s) 47, 98
BbvI GCAGC 1 cut(s) 253
BccI CCATC 2 cut(s) 440, 524
BceAI ACGGC 1 cut(s) 127
BciT130I CCWGG 3 cut(s) 152, 470, 711
BcnI CCSGG 3 cut(s) 355, 356, 392
BfmI CTRYAG 1 cut(s) 364
BglI GCCNNNNNGGC 1 cut(s) 428
BisI GCNGC 2 cut(s) 235, 267
BlsI GCNGC 2 cut(s) 236, 268
BmcAI AGTACT 1 cut(s) 545
Bme1390I CCNGG 6 cut(s) 152, 355, 356, 392, 470, 711
Bme18I GGWCC 1 cut(s) 480
BmeT110I CYCGRG 2 cut(s) 354, 668
BmgT120I GGNCC 4 cut(s) 148, 429, 480, 605
BmiI GGNNCC 3 cut(s) 76, 115, 481
BmrFI CCNGG 6 cut(s) 152, 355, 356, 392, 470, 711
BpmI CTGGAG 3 cut(s) 42, 93, 395
Bpu10I CCTNAGC 2 cut(s) 47, 98
BpuMI CCSGG 3 cut(s) 355, 356, 392
BsaAI YACGTR 1 cut(s) 42
BsaJI CCNNGG 3 cut(s) 354, 639, 700
Bsc4I CCNNNNNNNGG 5 cut(s) 20, 42, 469, 520, 580
Bse1I ACTGG 1 cut(s) 541
Bse3DI GCAATG 3 cut(s) 463, 532, 564
BseBI CCWGG 3 cut(s) 152, 470, 711
BseDI CCNNGG 3 cut(s) 354, 639, 700
BseGI GGATG 3 cut(s) 10, 93, 400
BseLI CCNNNNNNNGG 5 cut(s) 20, 42, 469, 520, 580
BseMI GCAATG 3 cut(s) 463, 532, 564
BseMII CTCAG 4 cut(s) 38, 112, 245, 609
BseNI ACTGG 1 cut(s) 541
BseRI GAGGAG 1 cut(s) 541
BseXI GCAGC 1 cut(s) 253
BseYI CCCAGC 1 cut(s) 145
Bsh1236I CGCG 1 cut(s) 108
BshFI GGCC 5 cut(s) 150, 413, 431, 540, 607
BshNI GGYRCC 1 cut(s) 113
BsiHKCI CYCGRG 2 cut(s) 354, 668
BsiSI CCGG 2 cut(s) 355, 392
BslI CCNNNNNNNGG 5 cut(s) 20, 42, 469, 520, 580
BsmI GAATGC 1 cut(s) 102
BsnI GGCC 5 cut(s) 150, 413, 431, 540, 607
BsoBI CYCGRG 2 cut(s) 354, 668
Bsp1286I GDGCHC 1 cut(s) 392
Bsp143I GATC 4 cut(s) 63, 121, 223, 379
Bsp19I CCATGG 1 cut(s) 700
Bsp68I TCGCGA 1 cut(s) 108
BspACI CCGC 3 cut(s) 129, 234, 591
BspANI GGCC 5 cut(s) 150, 413, 431, 540, 607
BspCNI CTCAG 4 cut(s) 39, 111, 246, 608
BspFNI CGCG 1 cut(s) 108
BspLI GGNNCC 3 cut(s) 76, 115, 481
BspMAI CTGCAG 1 cut(s) 368
BspPI GGATC 1 cut(s) 387
BspT107I GGYRCC 1 cut(s) 113
BsrDI GCAATG 3 cut(s) 463, 532, 564
BsrI ACTGG 1 cut(s) 541
BssECI CCNNGG 3 cut(s) 354, 639, 700
BssMI GATC 4 cut(s) 63, 121, 223, 379
BssNAI GTATAC 1 cut(s) 445
BssT1I CCWWGG 2 cut(s) 639, 700
Bst1107I GTATAC 1 cut(s) 445
Bst2UI CCWGG 3 cut(s) 152, 470, 711
Bst4CI ACNGT 3 cut(s) 59, 118, 602
BstBAI YACGTR 1 cut(s) 42
BstC8I GCNNGC 2 cut(s) 143, 511
BstDEI CTNAG 4 cut(s) 47, 98, 254, 595
BstDSI CCRYGG 1 cut(s) 700
BstEII GGTNACC 1 cut(s) 634
BstF5I GGATG 3 cut(s) 10, 93, 400
BstFNI CGCG 1 cut(s) 108
BstKTI GATC 4 cut(s) 66, 124, 226, 382
BstMBI GATC 4 cut(s) 63, 121, 223, 379
BstMWI GCNNNNNNNGC 7 cut(s) 52, 147, 419, 428, 437, 531, 546
BstNI CCWGG 3 cut(s) 152, 470, 711
BstPI GGTNACC 1 cut(s) 634
BstSCI CCNGG 6 cut(s) 150, 353, 354, 390, 468, 709
BstSFI CTRYAG 1 cut(s) 364
BstUI CGCG 1 cut(s) 108
BstV1I GCAGC 1 cut(s) 253
BstX2I RGATCY 1 cut(s) 379
BstYI RGATCY 1 cut(s) 379
BstZ17I GTATAC 1 cut(s) 445
BsuRI GGCC 5 cut(s) 150, 413, 431, 540, 607
BtgI CCRYGG 1 cut(s) 700
BtsCI GGATG 3 cut(s) 10, 93, 400
BtuMI TCGCGA 1 cut(s) 108
Cac8I GCNNGC 2 cut(s) 143, 511
Cfr13I GGNCC 4 cut(s) 148, 429, 480, 605
Cfr9I CCCGGG 1 cut(s) 354
CseI GACGC 1 cut(s) 723
Csp6I GTAC 4 cut(s) 179, 251, 544, 665
CviAII CATG 4 cut(s) 415, 694, 701, 726
CviQI GTAC 4 cut(s) 179, 251, 544, 665
DdeI CTNAG 4 cut(s) 47, 98, 254, 595
DpnI GATC 4 cut(s) 65, 123, 225, 381
DpnII GATC 4 cut(s) 63, 121, 223, 379
DrdI GACNNNNNNGTC 1 cut(s) 116
DseDI GACNNNNNNGTC 1 cut(s) 116
EaeI YGGCCR 2 cut(s) 411, 538
EciI GGCGGA 2 cut(s) 118, 580
Eco130I CCWWGG 2 cut(s) 639, 700
Eco24I GRGCYC 1 cut(s) 392
Eco32I GATATC 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 480
Eco57I CTGAAG 1 cut(s) 666
Eco72I CACGTG 1 cut(s) 42
Eco88I CYCGRG 2 cut(s) 354, 668
Eco91I GGTNACC 1 cut(s) 634
EcoO65I GGTNACC 1 cut(s) 634
EcoRI GAATTC 1 cut(s) 217
EcoRII CCWGG 3 cut(s) 150, 468, 709
EcoRV GATATC 1 cut(s) 241
EcoT14I CCWWGG 2 cut(s) 639, 700
EcoT38I GRGCYC 1 cut(s) 392
ErhI CCWWGG 2 cut(s) 639, 700
FaeI CATG 4 cut(s) 418, 697, 704, 729
FaiI YATR 8 cut(s) 161, 416, 445, 632, 675, 695, 702, 727
FatI CATG 4 cut(s) 414, 693, 700, 725
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 2 cut(s) 235, 267
FokI GGATG 3 cut(s) 17, 80, 407
FriOI GRGCYC 1 cut(s) 392
Fsp4HI GCNGC 2 cut(s) 235, 267
GluI GCNGC 2 cut(s) 235, 267
GsaI CCCAGC 1 cut(s) 149
GsuI CTGGAG 3 cut(s) 42, 93, 395
HaeIII GGCC 5 cut(s) 150, 413, 431, 540, 607
HapII CCGG 2 cut(s) 355, 392
HgaI GACGC 1 cut(s) 723
Hin1II CATG 4 cut(s) 418, 697, 704, 729
HindIII AAGCTT 1 cut(s) 207
HinfI GANTC 1 cut(s) 706
HpaII CCGG 2 cut(s) 355, 392
HphI GGTGA 1 cut(s) 628
Hpy166II GTNNAC 2 cut(s) 445, 665
Hpy188I TCNGA 6 cut(s) 63, 223, 273, 560, 598, 625
Hpy188III TCNNGA 4 cut(s) 107, 227, 341, 374
Hpy8I GTNNAC 2 cut(s) 445, 665
Hpy99I CGWCG 2 cut(s) 113, 122
HpyAV CCTTC 4 cut(s) 100, 142, 470, 610
HpyCH4III ACNGT 3 cut(s) 59, 118, 602
HpyCH4IV ACGT 1 cut(s) 41
HpyCH4V TGCA 2 cut(s) 366, 502
HpyF10VI GCNNNNNNNGC 7 cut(s) 52, 147, 419, 428, 437, 531, 546
HpyF3I CTNAG 4 cut(s) 47, 98, 254, 595
HpySE526I ACGT 1 cut(s) 41
Hsp92II CATG 4 cut(s) 418, 697, 704, 729
Kzo9I GATC 4 cut(s) 63, 121, 223, 379
LmnI GCTCC 3 cut(s) 74, 554, 727
Lsp1109I GCAGC 1 cut(s) 253
MaeII ACGT 1 cut(s) 41
MaeIII GTNAC 1 cut(s) 634
MalI GATC 4 cut(s) 65, 123, 225, 381
MboI GATC 4 cut(s) 63, 121, 223, 379
MboII GAAGA 3 cut(s) 226, 485, 638
MflI RGATCY 1 cut(s) 379
MhlI GDGCHC 1 cut(s) 392
MlsI TGGCCA 1 cut(s) 413
MluCI AATT 2 cut(s) 217, 245
MluNI TGGCCA 1 cut(s) 413
MlyI GAGTC 1 cut(s) 715
MmeI TCCRAC 1 cut(s) 561
MnlI CCTC 8 cut(s) 42, 107, 295, 370, 499, 562, 604, 670
Mox20I TGGCCA 1 cut(s) 413
MscI TGGCCA 1 cut(s) 413
MslI CAYNNNNRTG 1 cut(s) 164
Msp20I TGGCCA 1 cut(s) 413
MspI CCGG 2 cut(s) 355, 392
MspR9I CCNGG 6 cut(s) 152, 355, 356, 392, 470, 711
Mva1269I GAATGC 1 cut(s) 102
MvaI CCWGG 3 cut(s) 152, 470, 711
MvnI CGCG 1 cut(s) 108
MwoI GCNNNNNNNGC 7 cut(s) 52, 147, 419, 428, 437, 531, 546
NciI CCSGG 3 cut(s) 355, 356, 392
NcoI CCATGG 1 cut(s) 700
NdeII GATC 4 cut(s) 63, 121, 223, 379
NlaIII CATG 4 cut(s) 418, 697, 704, 729
NlaIV GGNNCC 3 cut(s) 76, 115, 481
NmuCI GTSAC 1 cut(s) 634
NruI TCGCGA 1 cut(s) 108
PaeR7I CTCGAG 1 cut(s) 668
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 234
PctI GAATGC 1 cut(s) 102
PflMI CCANNNNNTGG 2 cut(s) 42, 469
PfoI TCCNGGA 1 cut(s) 709
PkrI GCNGC 2 cut(s) 236, 268
PleI GAGTC 1 cut(s) 714
PmaCI CACGTG 1 cut(s) 42
PmlI CACGTG 1 cut(s) 42
PpsI GAGTC 1 cut(s) 714
Ppu21I YACGTR 1 cut(s) 42
Psp6I CCWGG 3 cut(s) 150, 468, 709
PspCI CACGTG 1 cut(s) 42
PspEI GGTNACC 1 cut(s) 634
PspFI CCCAGC 1 cut(s) 145
PspGI CCWGG 3 cut(s) 150, 468, 709
PspN4I GGNNCC 3 cut(s) 76, 115, 481
PspPI GGNCC 4 cut(s) 148, 429, 480, 605
PspXI VCTCGAGB 1 cut(s) 668
PstI CTGCAG 1 cut(s) 368
PsuI RGATCY 1 cut(s) 379
RruI TCGCGA 1 cut(s) 108
RsaI GTAC 4 cut(s) 180, 252, 545, 666
RsaNI GTAC 4 cut(s) 179, 251, 544, 665
RseI CAYNNNNRTG 1 cut(s) 164
SatI GCNGC 2 cut(s) 235, 267
Sau3AI GATC 4 cut(s) 63, 121, 223, 379
Sau96I GGNCC 4 cut(s) 148, 429, 480, 605
ScaI AGTACT 1 cut(s) 545
SchI GAGTC 1 cut(s) 715
ScrFI CCNGG 6 cut(s) 152, 355, 356, 392, 470, 711
SduI GDGCHC 1 cut(s) 392
SetI ASST 9 cut(s) 22, 44, 211, 327, 442, 458, 521, 621, 681
SfcI CTRYAG 1 cut(s) 364
Sfr274I CTCGAG 1 cut(s) 668
SinI GGWCC 1 cut(s) 480
SlaI CTCGAG 1 cut(s) 668
SmaI CCCGGG 1 cut(s) 356
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 668
SmoI CTYRAG 1 cut(s) 668
Sse9I AATT 2 cut(s) 217, 245
SsiI CCGC 3 cut(s) 129, 234, 591
StyD4I CCNGG 6 cut(s) 150, 353, 354, 390, 468, 709
StyI CCWWGG 2 cut(s) 639, 700
TaaI ACNGT 3 cut(s) 59, 118, 602
TaiI ACGT 1 cut(s) 44
TaqI TCGA 5 cut(s) 29, 120, 243, 435, 669
TaqII GACCGA 1 cut(s) 588
TasI AATT 2 cut(s) 217, 245
TatI WGTACW 2 cut(s) 543, 664
TauI GCSGC 1 cut(s) 237
TseFI GTSAC 1 cut(s) 634
TseI GCWGC 1 cut(s) 266
Tsp45I GTSAC 1 cut(s) 634
TspDTI ATGAA 1 cut(s) 82
TspMI CCCGGG 1 cut(s) 354
Van91I CCANNNNNTGG 2 cut(s) 42, 469
VpaK11BI GGWCC 1 cut(s) 480
XapI RAATTY 1 cut(s) 217
XcmI CCANNNNNNNNNTGG 1 cut(s) 707
XhoI CTCGAG 1 cut(s) 668
XmaI CCCGGG 1 cut(s) 354
XmiI GTMKAC 1 cut(s) 444
ZrmI AGTACT 1 cut(s) 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.