FvH4_4g20570

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006593
Physical Location & Seq
Forward (+)
23745872 .. 23749736
3865 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g20570.t1

Sequence Viewer

Length: 783 bp
ATGTGTTGGGCTGCATGTGAGACATATTGGTTTGCACTGCAAGACATTTGTTGTTTCTTGTGGTACAAGTTGAGCAACATTAAGAGGGTAAACCATCGTCGCAGGCGTCATCTCCGCCACCGCCAATTTCGAGACATTGAAGAAGGCTACAGTTCAAGTGGAGATAATAATTTAGGGGACGATTATAATAATCTTAATGTTAGTAGAAAGCGAAAGTCACTAAGCAGGAGAAGAATAGATAGGGTGCAGAGGTCTACTCACCCTTTGAGTAGGCATGGCAGTCGAAGTCACCATCATCGGCATGTAAGGTTAAAGACTAGGAGTATGTATGTTCGTGTTATGGGCAGGTCTCAAAGACTAAAAAGCTCGAGACGTGTGAAAATCAGCAAGGTGACAAATGTTCAAAGGAAAACCAAAACCTCTAAGAGGAGGAGGCTTAGGGTGAAGTACTTAATTAAGAATGGGTTGTCACGGAGGGATATTGCATTCATGGTTCTACAATTTTCTCCCTTGCTTGAGTACATCGTCGAAGAGGTTTTACGACCGAAGCTTGAATTCCTCATAAACACCATAGGGAAGCTGATAACAGAAGTCGTGGACTATCCGAGATACTTTAGTTATTCATTAAAAAAGAAGATAAAACCAAGATACTTGGTGCTAAAGGGAAAGAATATTGAGTGTAGCTTAAAGGATATGTTGGCTAAAAATGACGACGAATTTGCTTGCAAGTTCTTGAATGTTGAAAATATGCTTGTTACATGTCCTCCACCTTCACATCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

261

Amino Acids

31.18

Weight (kDa)

10.59

Isoelectric Point (pI)

67.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTERF PF02536 146 - 241 2.6e-31 mTERF
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 186
Acc36I ACCTGC 1 cut(s) 336
AccI GTMKAC 1 cut(s) 254
AciI CCGC 2 cut(s) 115, 121
AcsI RAATTY 2 cut(s) 554, 716
AcyI GRCGYC 1 cut(s) 106
AfaI GTAC 3 cut(s) 65, 449, 521
AfiI CCNNNNNNNGG 1 cut(s) 426
AflIII ACRYGT 2 cut(s) 373, 758
AgsI TTSAA 6 cut(s) 140, 156, 404, 554, 736, 743
AjiI CACGTC 1 cut(s) 374
AluBI AGCT 4 cut(s) 366, 550, 580, 684
AluI AGCT 4 cut(s) 366, 550, 580, 684
Alw26I GTCTC 4 cut(s) 14, 126, 354, 364
Ama87I CYCGRG 1 cut(s) 367
ApeKI GCWGC 1 cut(s) 11
ApoI RAATTY 2 cut(s) 554, 716
ArsI GACNNNNNNTTYG 2 cut(s) 701, 733
AsuHPI GGTGA 4 cut(s) 251, 281, 403, 454
AvaI CYCGRG 1 cut(s) 367
BaeI ACNNNNGTAYC 2 cut(s) 601, 634
BarI GAAGNNNNNNTAC 2 cut(s) 522, 554
BccI CCATC 2 cut(s) 102, 300
BcgI CGANNNNNNTGC 4 cut(s) 263, 297, 701, 735
BcoDI GTCTC 4 cut(s) 14, 126, 354, 364
BfaI CTAG 1 cut(s) 318
BfmI CTRYAG 1 cut(s) 148
BfuAI ACCTGC 1 cut(s) 336
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
BmcAI AGTACT 1 cut(s) 449
BmeT110I CYCGRG 1 cut(s) 367
BmgBI CACGTC 1 cut(s) 374
BplI GAGNNNNNCTC 2 cut(s) 241, 273
Bpu10I CCTNAGC 1 cut(s) 437
BpuEI CTTGAG 1 cut(s) 536
BsaHI GRCGYC 1 cut(s) 106
BsaI GGTCTC 1 cut(s) 354
BsaXI ACNNNNNCTCC 2 cut(s) 748, 778
Bsc4I CCNNNNNNNGG 1 cut(s) 426
BseLI CCNNNNNNNGG 1 cut(s) 426
BseRI GAGGAG 2 cut(s) 442, 445
BsgI GTGCAG 1 cut(s) 266
Bsh1285I CGRYCG 1 cut(s) 545
BsiEI CGRYCG 1 cut(s) 545
BsiHKCI CYCGRG 1 cut(s) 367
BslFI GGGAC 1 cut(s) 191
BslI CCNNNNNNNGG 1 cut(s) 426
BsmAI GTCTC 4 cut(s) 14, 126, 354, 364
BsmBI CGTCTC 1 cut(s) 364
BsmFI GGGAC 1 cut(s) 191
BsmI GAATGC 1 cut(s) 485
Bso31I GGTCTC 1 cut(s) 354
BsoBI CYCGRG 1 cut(s) 367
BspACI CCGC 2 cut(s) 115, 121
BspMI ACCTGC 1 cut(s) 336
BspTNI GGTCTC 1 cut(s) 354
BssNI GRCGYC 1 cut(s) 106
Bst4CI ACNGT 1 cut(s) 152
Bst6I CTCTTC 1 cut(s) 525
BstACI GRCGYC 1 cut(s) 106
BstC8I GCNNGC 2 cut(s) 104, 724
BstDEI CTNAG 3 cut(s) 221, 423, 437
BstENI CCTNNNNNAGG 1 cut(s) 424
BstMAI GTCTC 4 cut(s) 14, 126, 354, 364
BstMCI CGRYCG 1 cut(s) 545
BstNSI RCATGY 3 cut(s) 18, 305, 762
BstSFI CTRYAG 1 cut(s) 148
BtrI CACGTC 1 cut(s) 374
BtsI GCAGTG 1 cut(s) 35
BtsIMutI CAGTG 1 cut(s) 35
BveI ACCTGC 1 cut(s) 336
Cac8I GCNNGC 2 cut(s) 104, 724
CseI GACGC 1 cut(s) 95
Csp6I GTAC 3 cut(s) 64, 448, 520
CviAII CATG 5 cut(s) 15, 275, 302, 490, 759
CviJI RGCY 8 cut(s) 11, 147, 366, 436, 550, 580, 684, 701
CviKI_1 RGCY 8 cut(s) 11, 147, 366, 436, 550, 580, 684, 701
CviQI GTAC 3 cut(s) 64, 448, 520
DdeI CTNAG 3 cut(s) 221, 423, 437
Eam1104I CTCTTC 1 cut(s) 525
EarI CTCTTC 1 cut(s) 525
EciI GGCGGA 1 cut(s) 104
Eco31I GGTCTC 1 cut(s) 354
Eco88I CYCGRG 1 cut(s) 367
EcoNI CCTNNNNNAGG 1 cut(s) 424
EcoRI GAATTC 1 cut(s) 554
Esp3I CGTCTC 1 cut(s) 364
FaeI CATG 5 cut(s) 18, 278, 305, 493, 762
FaqI GGGAC 1 cut(s) 191
FatI CATG 5 cut(s) 14, 274, 301, 489, 758
FblI GTMKAC 1 cut(s) 254
Fnu4HI GCNGC 1 cut(s) 12
Fsp4HI GCNGC 1 cut(s) 12
FspBI CTAG 1 cut(s) 318
GluI GCNGC 1 cut(s) 12
HgaI GACGC 1 cut(s) 95
Hin1I GRCGYC 1 cut(s) 106
Hin1II CATG 5 cut(s) 18, 278, 305, 493, 762
HindIII AAGCTT 1 cut(s) 548
HphI GGTGA 4 cut(s) 251, 281, 403, 454
Hpy166II GTNNAC 3 cut(s) 91, 255, 598
Hpy188I TCNGA 1 cut(s) 606
Hpy188III TCNNGA 3 cut(s) 131, 369, 733
Hpy8I GTNNAC 3 cut(s) 91, 255, 598
Hpy99I CGWCG 3 cut(s) 102, 530, 716
HpyAV CCTTC 2 cut(s) 137, 780
HpyCH4III ACNGT 1 cut(s) 152
HpyCH4IV ACGT 1 cut(s) 373
HpyCH4V TGCA 6 cut(s) 14, 35, 40, 247, 485, 726
HpyF3I CTNAG 3 cut(s) 221, 423, 437
HpySE526I ACGT 1 cut(s) 373
Hsp92I GRCGYC 1 cut(s) 106
Hsp92II CATG 5 cut(s) 18, 278, 305, 493, 762
LpnPI CCDG 3 cut(s) 88, 211, 331
MaeI CTAG 1 cut(s) 318
MaeII ACGT 1 cut(s) 373
MaeIII GTNAC 5 cut(s) 216, 287, 391, 468, 754
MboII GAAGA 4 cut(s) 152, 243, 542, 646
MluCI AATT 6 cut(s) 125, 169, 453, 500, 554, 716
MseI TTAA 7 cut(s) 81, 195, 311, 452, 456, 626, 686
MslI CAYNNNNRTG 1 cut(s) 300
Mva1269I GAATGC 1 cut(s) 485
NlaIII CATG 5 cut(s) 18, 278, 305, 493, 762
NmuCI GTSAC 4 cut(s) 216, 287, 391, 468
NspI RCATGY 3 cut(s) 18, 305, 762
PacI TTAATTAA 1 cut(s) 456
PaeR7I CTCGAG 1 cut(s) 367
PciI ACATGT 1 cut(s) 758
PcsI WCGNNNNNNNCGW 1 cut(s) 103
PctI GAATGC 1 cut(s) 485
PkrI GCNGC 1 cut(s) 13
PscI ACATGT 1 cut(s) 758
PsiI TTATAA 1 cut(s) 186
RsaI GTAC 3 cut(s) 65, 449, 521
RsaNI GTAC 3 cut(s) 64, 448, 520
RseI CAYNNNNRTG 1 cut(s) 300
SaqAI TTAA 7 cut(s) 81, 195, 311, 452, 456, 626, 686
SatI GCNGC 1 cut(s) 12
ScaI AGTACT 1 cut(s) 449
SfcI CTRYAG 1 cut(s) 148
Sfr274I CTCGAG 1 cut(s) 367
SlaI CTCGAG 1 cut(s) 367
SmiMI CAYNNNNRTG 1 cut(s) 300
SmlI CTYRAG 2 cut(s) 367, 515
SmoI CTYRAG 2 cut(s) 367, 515
Sse9I AATT 6 cut(s) 125, 169, 453, 500, 554, 716
SsiI CCGC 2 cut(s) 115, 121
SspI AATATT 1 cut(s) 673
SspMI CTAG 1 cut(s) 318
TaaI ACNGT 1 cut(s) 152
TaiI ACGT 1 cut(s) 376
TaqI TCGA 4 cut(s) 130, 283, 368, 528
TaqII GACCGA 1 cut(s) 559
TasI AATT 6 cut(s) 125, 169, 453, 500, 554, 716
TatI WGTACW 2 cut(s) 447, 519
Tru1I TTAA 7 cut(s) 81, 195, 311, 452, 456, 626, 686
Tru9I TTAA 7 cut(s) 81, 195, 311, 452, 456, 626, 686
TscAI CASTG 1 cut(s) 42
TseFI GTSAC 4 cut(s) 216, 287, 391, 468
TseI GCWGC 1 cut(s) 11
Tsp45I GTSAC 4 cut(s) 216, 287, 391, 468
TspDTI ATGAA 2 cut(s) 478, 612
TspGWI ACGGA 1 cut(s) 487
TspRI CASTG 1 cut(s) 42
XagI CCTNNNNNAGG 1 cut(s) 424
XapI RAATTY 2 cut(s) 554, 716
XceI RCATGY 3 cut(s) 18, 305, 762
XhoI CTCGAG 1 cut(s) 367
XmiI GTMKAC 1 cut(s) 254
XspI CTAG 1 cut(s) 318
ZrmI AGTACT 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.