FvH4_4g21650

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013525
Physical Location & Seq
Forward (+)
24687653 .. 24689290
1638 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g21650.t1

Sequence Viewer

Length: 1065 bp
ATGGAGGTCTCCGGCTTGGTTTCCCGCCAAATTCCGTGCTTTTCCGGCGCGGGAATTGATACCCGGTCTTCCGGATCGTTTTCCGGCGAGGTCCATTTTCCGGGCCGGAATGAATATGGGGCGAGTTTGAGGAGCAGAAGGGTTGATATGAATTCTGGGTTTTGCGATAACGGGCATGTGGAGTATTATTATTCGGGTCCCAGATGCGGTGGTGGTAATAAGAAGGAGAAGGAGATCAAGAAGAAGTTGAAGTTGCTTAAAGGATTAGCGGAGCTGTCTTCGTCGACTCAGATCAGTACAGGGTTGGATTCCGAGGAGGGTTCGGCAGCTCAAGTCCAGAGGAAGCTGATTTCGGAGGGTGCAGAGGCATTGCTGCAGCAATTGGAACAGGTGAGAGCTGAGGAGAAGGAGATGAAGAAAAAGAAGAAAGAAGAGAAGGCGAGGCTTAAAGCCGAGCGGATGAAGACCATGAAGGACTGTGAGTCGTCGTCTTCATCTGAGTCGAGCGAGAGTGAATGTGGGGAGGTGATTGACATGAACAGGGTGAGAAGTGAAGTTCCCAAGCAAGCGATGCTAGAGGGTGTGAAGCCATTGGCTCAAGAACGGGTGGCATTGTTGACCCAACCGAGTTTGGTAACCACACTTCAAGAAGATAAATGCTGCAATGGAACTAGTACTAGTTTTGGCAGCAGTGATAGTATTGATCTTAACAATGCTTCTTCTAGCTCAGCTACAGAAGCATCTATCGTGGCGAAGGTTGAAGTTTGCATGGGCAAGAAGTGCAAGACATCAGGAGGTGTAGAATTGTTGGAGGAATTCGAGAGGTTGATGGGTGTCCAAGGCAGTGTTGTTGGGTGTAAGTGTTTGGGAAAATGCAAAAATGGTCCGAATGTCAGGGTTGTGAATTCCGTAAATGGGATCAAATCCGAAGAAGGAACAGAGGATTCTGTAAGGAATCCCACTAATCCTTTGTACATTGGAGTTGGCCTGGAAGATGTGAGCCTCATTGTGGCCAACTTAATGGGGGAAGTCAAGAAGGATTTGGGGGTTGCCACTGCAATTTAG

Protein Analysis

355

Amino Acids

37.99

Weight (kDa)

6.32

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013525)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48300
fragaria_vesca FvH4_4g21650 FvH4_4g21660
malus_domestica MD13G1156700.v1.1 MD16G1134300.v1.1
prunus_persica Prupe.1G103600_v2.0.a1 Prupe.1G103600_v2.0.a1
pyrus_communis pycom13g12050 pycom16g11860
rosa_chinensis RchiOBHm_Chr4g0427881
rosa_multiflora Rmu_co8387153.1_g000001 Rmu_sc0000066.1_g000016
rosa_roxburghii Rroxscaffold_5G00369530
rosa_rugosa Rorug04G0220200
rosa_samantha Rh4AG277200 Rh4BG283000 Rh4DG280100
rosa_wichuraiana Rw4G024080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 457
AccI GTMKAC 1 cut(s) 284
AccII CGCG 1 cut(s) 50
AccIII TCCGGA 1 cut(s) 71
AciI CCGC 5 cut(s) 25, 50, 207, 269, 457
AclWI GGATC 2 cut(s) 82, 926
AcoI YGGCCR 1 cut(s) 1011
AcsI RAATTY 4 cut(s) 30, 151, 815, 904
AfaI GTAC 3 cut(s) 298, 676, 974
AfiI CCNNNNNNNGG 4 cut(s) 100, 206, 915, 1009
AgsI TTSAA 3 cut(s) 250, 647, 761
AhdI GACNNNNNGTC 1 cut(s) 481
AhlI ACTAGT 2 cut(s) 671, 677
AjnI CCWGG 1 cut(s) 987
AluBI AGCT 6 cut(s) 274, 329, 346, 398, 726, 731
AluI AGCT 6 cut(s) 274, 329, 346, 398, 726, 731
Alw26I GTCTC 1 cut(s) 13
AlwI GGATC 2 cut(s) 82, 926
Aor13HI TCCGGA 1 cut(s) 71
AoxI GGCC 3 cut(s) 103, 985, 1011
ApeKI GCWGC 5 cut(s) 326, 373, 376, 660, 687
ApoI RAATTY 4 cut(s) 30, 151, 815, 904
AspLEI GCGC 1 cut(s) 50
AspS9I GGNCC 4 cut(s) 91, 103, 197, 884
AsuC2I CCSGG 2 cut(s) 64, 102
AsuHPI GGTGA 3 cut(s) 403, 538, 556
AvaII GGWCC 3 cut(s) 91, 197, 884
BalI TGGCCA 1 cut(s) 1013
BarI GAAGNNNNNNTAC 2 cut(s) 627, 659
BbsI GAAGAC 4 cut(s) 60, 270, 470, 483
BbvCI CCTCAGC 1 cut(s) 399
BbvI GCAGC 5 cut(s) 338, 360, 388, 647, 699
BccI CCATC 1 cut(s) 823
BciT130I CCWGG 1 cut(s) 989
BcnI CCSGG 2 cut(s) 64, 102
BcoDI GTCTC 1 cut(s) 13
BcuI ACTAGT 2 cut(s) 671, 677
BfaI CTAG 4 cut(s) 575, 672, 678, 723
BfmI CTRYAG 2 cut(s) 374, 732
BisI GCNGC 5 cut(s) 327, 374, 377, 661, 688
BlpI GCTNAGC 1 cut(s) 727
BlsI GCNGC 5 cut(s) 328, 375, 378, 662, 689
BmcAI AGTACT 1 cut(s) 676
Bme1390I CCNGG 3 cut(s) 64, 102, 989
Bme18I GGWCC 3 cut(s) 91, 197, 884
BmeRI GACNNNNNGTC 1 cut(s) 481
BmgT120I GGNCC 4 cut(s) 91, 103, 197, 884
BmiI GGNNCC 2 cut(s) 198, 199
BmrFI CCNGG 3 cut(s) 64, 102, 989
BmsI GCATC 3 cut(s) 194, 561, 749
BpiI GAAGAC 4 cut(s) 60, 270, 470, 483
Bpu10I CCTNAGC 1 cut(s) 399
Bpu1102I GCTNAGC 1 cut(s) 727
BpuEI CTTGAG 2 cut(s) 315, 582
BpuMI CCSGG 2 cut(s) 64, 102
BsaI GGTCTC 1 cut(s) 13
BsaJI CCNNGG 2 cut(s) 312, 838
BsaWI WCCGGW 1 cut(s) 71
Bsc4I CCNNNNNNNGG 4 cut(s) 100, 206, 915, 1009
Bse3DI GCAATG 2 cut(s) 368, 670
BseAI TCCGGA 1 cut(s) 71
BseBI CCWGG 1 cut(s) 989
BseDI CCNNGG 2 cut(s) 312, 838
BseGI GGATG 1 cut(s) 465
BseLI CCNNNNNNNGG 4 cut(s) 100, 206, 915, 1009
BseMI GCAATG 2 cut(s) 368, 670
BseMII CTCAG 4 cut(s) 302, 390, 489, 741
BseRI GAGGAG 3 cut(s) 145, 329, 416
BseXI GCAGC 5 cut(s) 338, 360, 388, 647, 699
BsgI GTGCAG 1 cut(s) 381
Bsh1236I CGCG 1 cut(s) 50
BshFI GGCC 3 cut(s) 105, 987, 1013
BsiSI CCGG 7 cut(s) 12, 45, 64, 72, 84, 101, 106
BslFI GGGAC 1 cut(s) 183
BslI CCNNNNNNNGG 4 cut(s) 100, 206, 915, 1009
BsmAI GTCTC 1 cut(s) 13
BsmFI GGGAC 1 cut(s) 183
BsnI GGCC 3 cut(s) 105, 987, 1013
Bso31I GGTCTC 1 cut(s) 13
Bsp13I TCCGGA 1 cut(s) 71
Bsp1407I TGTACA 1 cut(s) 972
Bsp143I GATC 5 cut(s) 74, 234, 291, 703, 918
Bsp1720I GCTNAGC 1 cut(s) 727
BspACI CCGC 5 cut(s) 25, 50, 207, 269, 457
BspANI GGCC 3 cut(s) 105, 987, 1013
BspCNI CTCAG 4 cut(s) 301, 391, 490, 740
BspEI TCCGGA 1 cut(s) 71
BspFNI CGCG 1 cut(s) 50
BspLI GGNNCC 2 cut(s) 198, 199
BspMAI CTGCAG 1 cut(s) 378
BspPI GGATC 2 cut(s) 82, 926
BspTNI GGTCTC 1 cut(s) 13
BsrBI CCGCTC 1 cut(s) 457
BsrDI GCAATG 2 cut(s) 368, 670
BsrGI TGTACA 1 cut(s) 972
BssECI CCNNGG 2 cut(s) 312, 838
BssMI GATC 5 cut(s) 74, 234, 291, 703, 918
BssT1I CCWWGG 1 cut(s) 838
Bst2UI CCWGG 1 cut(s) 989
Bst4CI ACNGT 1 cut(s) 479
Bst6I CTCTTC 1 cut(s) 426
BstAPI GCANNNNNTGC 2 cut(s) 571, 780
BstAUI TGTACA 1 cut(s) 972
BstC8I GCNNGC 1 cut(s) 567
BstDEI CTNAG 4 cut(s) 288, 399, 498, 727
BstEII GGTNACC 1 cut(s) 634
BstF5I GGATG 1 cut(s) 465
BstFNI CGCG 1 cut(s) 50
BstHHI GCGC 1 cut(s) 50
BstKTI GATC 5 cut(s) 77, 237, 294, 706, 921
BstMAI GTCTC 1 cut(s) 13
BstMBI GATC 5 cut(s) 74, 234, 291, 703, 918
BstMWI GCNNNNNNNGC 4 cut(s) 45, 571, 737, 780
BstNI CCWGG 1 cut(s) 989
BstNSI RCATGY 1 cut(s) 179
BstPI GGTNACC 1 cut(s) 634
BstSCI CCNGG 3 cut(s) 62, 100, 987
BstSFI CTRYAG 2 cut(s) 374, 732
BstUI CGCG 1 cut(s) 50
BstV1I GCAGC 5 cut(s) 338, 360, 388, 647, 699
BstV2I GAAGAC 4 cut(s) 60, 270, 470, 483
BstXI CCANNNNNNTGG 1 cut(s) 1021
BsuRI GGCC 3 cut(s) 105, 987, 1013
BtgZI GCGATG 1 cut(s) 584
BtsCI GGATG 1 cut(s) 465
BtsI GCAGTG 3 cut(s) 697, 850, 1053
BtsIMutI CAGTG 3 cut(s) 697, 850, 1053
Cac8I GCNNGC 1 cut(s) 567
CfoI GCGC 1 cut(s) 50
Cfr13I GGNCC 4 cut(s) 91, 103, 197, 884
Csp6I GTAC 3 cut(s) 297, 675, 973
CviAII CATG 4 cut(s) 176, 469, 535, 769
CviQI GTAC 3 cut(s) 297, 675, 973
DdeI CTNAG 4 cut(s) 288, 399, 498, 727
DpnI GATC 5 cut(s) 76, 236, 293, 705, 920
DpnII GATC 5 cut(s) 74, 234, 291, 703, 918
DriI GACNNNNNGTC 1 cut(s) 481
EaeI YGGCCR 1 cut(s) 1011
Eam1104I CTCTTC 1 cut(s) 426
Eam1105I GACNNNNNGTC 1 cut(s) 481
EarI CTCTTC 1 cut(s) 426
Eco130I CCWWGG 1 cut(s) 838
Eco31I GGTCTC 1 cut(s) 13
Eco47I GGWCC 3 cut(s) 91, 197, 884
Eco91I GGTNACC 1 cut(s) 634
EcoO109I RGGNCCY 1 cut(s) 197
EcoO65I GGTNACC 1 cut(s) 634
EcoRI GAATTC 3 cut(s) 151, 815, 904
EcoRII CCWGG 1 cut(s) 987
EcoT14I CCWWGG 1 cut(s) 838
ErhI CCWWGG 1 cut(s) 838
FaeI CATG 4 cut(s) 179, 472, 538, 772
FaiI YATR 6 cut(s) 117, 149, 177, 470, 536, 770
FaqI GGGAC 1 cut(s) 183
FatI CATG 4 cut(s) 175, 468, 534, 768
FauI CCCGC 2 cut(s) 32, 43
FblI GTMKAC 1 cut(s) 284
Fnu4HI GCNGC 5 cut(s) 327, 374, 377, 661, 688
FokI GGATG 1 cut(s) 472
Fsp4HI GCNGC 5 cut(s) 327, 374, 377, 661, 688
FspBI CTAG 4 cut(s) 575, 672, 678, 723
GlaI GCGC 1 cut(s) 49
GluI GCNGC 5 cut(s) 327, 374, 377, 661, 688
HaeIII GGCC 3 cut(s) 105, 987, 1013
HapII CCGG 7 cut(s) 12, 45, 64, 72, 84, 101, 106
HhaI GCGC 1 cut(s) 50
Hin1II CATG 4 cut(s) 179, 472, 538, 772
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HincII GTYRAC 2 cut(s) 285, 618
HindII GTYRAC 2 cut(s) 285, 618
HinfI GANTC 6 cut(s) 286, 308, 482, 500, 944, 955
HpaII CCGG 7 cut(s) 12, 45, 64, 72, 84, 101, 106
HphI GGTGA 3 cut(s) 403, 538, 556
Hpy166II GTNNAC 2 cut(s) 285, 618
Hpy188I TCNGA 6 cut(s) 291, 313, 355, 499, 888, 928
Hpy188III TCNNGA 8 cut(s) 72, 238, 337, 599, 647, 792, 820, 1033
Hpy8I GTNNAC 2 cut(s) 285, 618
Hpy99I CGWCG 2 cut(s) 286, 490
HpyAV CCTTC 9 cut(s) 132, 217, 223, 400, 430, 466, 748, 926, 1030
HpyCH4III ACNGT 1 cut(s) 479
HpyCH4V TGCA 7 cut(s) 362, 376, 663, 768, 783, 876, 1058
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 571, 737, 780
HpyF3I CTNAG 4 cut(s) 288, 399, 498, 727
Hsp92II CATG 4 cut(s) 179, 472, 538, 772
HspAI GCGC 1 cut(s) 48
KflI GGGWCCC 1 cut(s) 197
Kpn2I TCCGGA 1 cut(s) 71
Kzo9I GATC 5 cut(s) 74, 234, 291, 703, 918
LmnI GCTCC 2 cut(s) 132, 271
Lsp1109I GCAGC 5 cut(s) 338, 360, 388, 647, 699
LweI GCATC 3 cut(s) 194, 561, 749
MaeI CTAG 4 cut(s) 575, 672, 678, 723
MaeIII GTNAC 1 cut(s) 634
MalI GATC 5 cut(s) 76, 236, 293, 705, 920
MbiI CCGCTC 1 cut(s) 457
MboI GATC 5 cut(s) 74, 234, 291, 703, 918
MfeI CAATTG 1 cut(s) 380
MlsI TGGCCA 1 cut(s) 1013
MluCI AATT 8 cut(s) 30, 54, 151, 380, 803, 815, 904, 1059
MluNI TGGCCA 1 cut(s) 1013
MlyI GAGTC 3 cut(s) 280, 491, 509
MmeI TCCRAC 2 cut(s) 285, 789
Mox20I TGGCCA 1 cut(s) 1013
MroI TCCGGA 1 cut(s) 71
MscI TGGCCA 1 cut(s) 1013
MseI TTAA 4 cut(s) 258, 447, 708, 1019
Msp20I TGGCCA 1 cut(s) 1013
MspI CCGG 7 cut(s) 12, 45, 64, 72, 84, 101, 106
MspR9I CCNGG 3 cut(s) 64, 102, 989
MunI CAATTG 1 cut(s) 380
MvaI CCWGG 1 cut(s) 989
MvnI CGCG 1 cut(s) 50
MwoI GCNNNNNNNGC 4 cut(s) 45, 571, 737, 780
NciI CCSGG 2 cut(s) 64, 102
NdeII GATC 5 cut(s) 74, 234, 291, 703, 918
NlaIII CATG 4 cut(s) 179, 472, 538, 772
NlaIV GGNNCC 2 cut(s) 198, 199
NmeAIII GCCGAG 1 cut(s) 478
NspI RCATGY 1 cut(s) 179
PfeI GAWTC 3 cut(s) 308, 944, 955
PkrI GCNGC 5 cut(s) 328, 375, 378, 662, 689
PleI GAGTC 3 cut(s) 280, 490, 508
PpsI GAGTC 3 cut(s) 280, 490, 508
PpuMI RGGWCCY 1 cut(s) 197
Psp5II RGGWCCY 1 cut(s) 197
Psp6I CCWGG 1 cut(s) 987
PspEI GGTNACC 1 cut(s) 634
PspGI CCWGG 1 cut(s) 987
PspN4I GGNNCC 2 cut(s) 198, 199
PspPI GGNCC 4 cut(s) 91, 103, 197, 884
PspPPI RGGWCCY 1 cut(s) 197
PstI CTGCAG 1 cut(s) 378
RsaI GTAC 3 cut(s) 298, 676, 974
RsaNI GTAC 3 cut(s) 297, 675, 973
SalI GTCGAC 1 cut(s) 283
SaqAI TTAA 4 cut(s) 258, 447, 708, 1019
SatI GCNGC 5 cut(s) 327, 374, 377, 661, 688
Sau3AI GATC 5 cut(s) 74, 234, 291, 703, 918
Sau96I GGNCC 4 cut(s) 91, 103, 197, 884
ScaI AGTACT 1 cut(s) 676
SchI GAGTC 3 cut(s) 280, 491, 509
ScrFI CCNGG 3 cut(s) 64, 102, 989
SfaNI GCATC 3 cut(s) 194, 561, 749
SfcI CTRYAG 2 cut(s) 374, 732
SinI GGWCC 3 cut(s) 91, 197, 884
SmlI CTYRAG 2 cut(s) 330, 597
SmoI CTYRAG 2 cut(s) 330, 597
SpeI ACTAGT 2 cut(s) 671, 677
Sse9I AATT 8 cut(s) 30, 54, 151, 380, 803, 815, 904, 1059
SsiI CCGC 5 cut(s) 25, 50, 207, 269, 457
SspMI CTAG 4 cut(s) 575, 672, 678, 723
StyD4I CCNGG 3 cut(s) 62, 100, 987
StyI CCWWGG 1 cut(s) 838
TaaI ACNGT 1 cut(s) 479
TaqI TCGA 3 cut(s) 284, 503, 819
TasI AATT 8 cut(s) 30, 54, 151, 380, 803, 815, 904, 1059
TatI WGTACW 3 cut(s) 296, 674, 972
TfiI GAWTC 3 cut(s) 308, 944, 955
Tru1I TTAA 4 cut(s) 258, 447, 708, 1019
Tru9I TTAA 4 cut(s) 258, 447, 708, 1019
TscAI CASTG 3 cut(s) 697, 850, 1060
TseI GCWGC 5 cut(s) 326, 373, 376, 660, 687
TspDTI ATGAA 7 cut(s) 126, 164, 428, 476, 483, 485, 551
TspGWI ACGGA 2 cut(s) 24, 898
TspRI CASTG 3 cut(s) 697, 850, 1060
VpaK11BI GGWCC 3 cut(s) 91, 197, 884
XapI RAATTY 4 cut(s) 30, 151, 815, 904
XceI RCATGY 1 cut(s) 179
XmiI GTMKAC 1 cut(s) 284
XspI CTAG 4 cut(s) 575, 672, 678, 723
ZrmI AGTACT 1 cut(s) 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.