MD13G1156700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013525
Physical Location & Seq
Forward (+)
12231609 .. 12233455
1847 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1156700.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGGAGGCCTCCGGCGTCGTTTCCCGCCAAGTTCAGTGCTTTTCCGCTGCTAAAATCGACAACTCCGCCCGTTCTTGTACGTCGTCGTTTAACTGCAAGCTTCGCGTTCTGGGTCGTCGTGATTTTGGAGTTTCACTGAGAACCAGAAAGCCTCGCCGGCATTTAAATTCCGGGTTTAGCGACGACGGACATTTACAGTATTATCAGGCGGGTCCCATGTGCGGTGGTATGAAGGAGAAGGCGAAGGAGATCAAGAAGAAGGTGAAGTTGCTCAAGGGGTTGTCGAAGAACTTGGCGGCGTATTCTCAGATGGGTTTTGGACCGTTGGATTCTCAAGAGGGTTTGGTCGATCAACTTCAGGGGAAGCTGATCTCGGAAAGTGCTGAGGAATTATTGCTAAAACAATTGGAACAGCTAAGAGCAGAGGAAAAGGAATTGAAGAAGAAGGGGAAAGAAGAGAAGGCAAGGCTTAAAGCTGAGCGGATGAAAACCATGGTTGACAGTGAATCATCATCGTCTTCATCGTCTGAATCCAGTGACAGTGAGTGTGGGGAGGTGGTAGACATGAAGCGCCTGCAAAGTGAAGTCCCGGCGCAACCAATTGTAGATAGTTTGCAGCCATTTGCTCATCAAGAAGGGGCATTGTTGACCATACTTAGCTCGCTAGCTACTCATCAAGAGAACACAACTGTGGAAACTGGCATTGATTTTGGAAGTTCACAGATAGATCAAAAAGCAGAATGCTGTAATGGAACTAGTACTATTTGTGGCAGTAGCGGTAGTATTACCTTTAATGAGTCTCTAAGTAGCGCAGTTGTCGGAGTTTCAGCGAAGAAGATTGAGGTGTGCATGGGAAACAAGTGCAAGAAATCAGGAGGCGGAGCGTTGTTGGAAGAATTCGAGAGGCAGATGGGCGGTGAAGGAGCTGTTGTAGGGTGCAAGTGTATGGGAAAATGCAAAAACGGCCCTAATGTGAGGGTTTCAACCACCGTTGGCAGGATTCAATCCGAAGGAATGGATGACTCCGTTAGAAGTCCGACGAATCCTTTGTACATTGGAGTTGGCCTGGAAGATGTGAGCCTCATTGTGGCTAATTTAATTGGGGACGAAAACAAGGATTTGGACCTTGTTTCTGCAGCTTAG

Protein Analysis

381

Amino Acids

40.75

Weight (kDa)

6.9

Isoelectric Point (pI)

50.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013525)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48300
fragaria_vesca FvH4_4g21650 FvH4_4g21660
malus_domestica MD13G1156700.v1.1 MD16G1134300.v1.1
prunus_persica Prupe.1G103600_v2.0.a1 Prupe.1G103600_v2.0.a1
pyrus_communis pycom13g12050 pycom16g11860
rosa_chinensis RchiOBHm_Chr4g0427881
rosa_multiflora Rmu_co8387153.1_g000001 Rmu_sc0000066.1_g000016
rosa_roxburghii Rroxscaffold_5G00369530
rosa_rugosa Rorug04G0220200
rosa_samantha Rh4AG277200 Rh4BG283000 Rh4DG280100
rosa_wichuraiana Rw4G024080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 481
AccI GTMKAC 1 cut(s) 561
AccII CGCG 1 cut(s) 105
AcsI RAATTY 2 cut(s) 166, 896
AcuI CTGAAG 1 cut(s) 341
AcyI GRCGYC 1 cut(s) 15
AfaI GTAC 3 cut(s) 79, 760, 1052
AfiI CCNNNNNNNGG 3 cut(s) 221, 996, 1087
AgsI TTSAA 3 cut(s) 439, 984, 1004
AhlI ACTAGT 1 cut(s) 755
AjnI CCWGG 1 cut(s) 1065
AleI CACNNNNGTG 1 cut(s) 689
AluBI AGCT 8 cut(s) 100, 367, 415, 476, 660, 668, 926, 1139
AluI AGCT 8 cut(s) 100, 367, 415, 476, 660, 668, 926, 1139
Alw26I GTCTC 1 cut(s) 804
AoxI GGCC 3 cut(s) 6, 964, 1063
ApeKI GCWGC 3 cut(s) 47, 616, 1136
ApoI RAATTY 2 cut(s) 166, 896
ArsI GACNNNNNNTTYG 2 cut(s) 1030, 1062
AspLEI GCGC 3 cut(s) 573, 595, 812
AspS9I GGNCC 4 cut(s) 212, 320, 965, 1123
AsuC2I CCSGG 2 cut(s) 172, 590
AsuHPI GGTGA 2 cut(s) 274, 929
AsuNHI GCTAGC 1 cut(s) 664
AvaII GGWCC 3 cut(s) 212, 320, 1123
BbsI GAAGAC 1 cut(s) 510
BbvCI CCTCAGC 1 cut(s) 384
BbvI GCAGC 2 cut(s) 34, 628
BccI CCATC 2 cut(s) 304, 904
BceAI ACGGC 1 cut(s) 979
BciT130I CCWGG 1 cut(s) 1067
BcnI CCSGG 2 cut(s) 172, 590
BcoDI GTCTC 1 cut(s) 804
BcuI ACTAGT 1 cut(s) 755
BfaI CTAG 2 cut(s) 665, 756
BfmI CTRYAG 1 cut(s) 1134
BfoI RGCGCY 1 cut(s) 574
BglI GCCNNNNNGGC 1 cut(s) 157
BisI GCNGC 4 cut(s) 48, 297, 617, 1137
BlpI GCTNAGC 1 cut(s) 477
BlsI GCNGC 4 cut(s) 49, 298, 618, 1138
BmcAI AGTACT 1 cut(s) 760
Bme1390I CCNGG 3 cut(s) 172, 590, 1067
Bme18I GGWCC 3 cut(s) 212, 320, 1123
BmgT120I GGNCC 4 cut(s) 212, 320, 965, 1123
BmiI GGNNCC 2 cut(s) 213, 214
BmrFI CCNGG 3 cut(s) 172, 590, 1067
BmtI GCTAGC 1 cut(s) 668
BpiI GAAGAC 1 cut(s) 510
Bpu10I CCTNAGC 1 cut(s) 384
Bpu1102I GCTNAGC 1 cut(s) 477
BpuEI CTTGAG 2 cut(s) 257, 318
BpuMI CCSGG 2 cut(s) 172, 590
BsaHI GRCGYC 1 cut(s) 15
BsaJI CCNNGG 1 cut(s) 492
BsaXI ACNNNNNCTCC 2 cut(s) 915, 945
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 996, 1087
Bse118I RCCGGY 1 cut(s) 156
Bse1I ACTGG 2 cut(s) 534, 703
BseBI CCWGG 1 cut(s) 1067
BseDI CCNNGG 1 cut(s) 492
BseGI GGATG 2 cut(s) 489, 1024
BseLI CCNNNNNNNGG 3 cut(s) 221, 996, 1087
BseMII CTCAG 4 cut(s) 128, 320, 375, 468
BseNI ACTGG 2 cut(s) 534, 703
BseXI GCAGC 2 cut(s) 34, 628
Bsh1236I CGCG 1 cut(s) 105
BshFI GGCC 3 cut(s) 8, 966, 1065
BsiSI CCGG 4 cut(s) 12, 157, 171, 590
BslFI GGGAC 3 cut(s) 198, 572, 1118
BslI CCNNNNNNNGG 3 cut(s) 221, 996, 1087
BsmAI GTCTC 1 cut(s) 804
BsmFI GGGAC 3 cut(s) 198, 572, 1118
BsmI GAATGC 1 cut(s) 746
BsnI GGCC 3 cut(s) 8, 966, 1065
Bsp1407I TGTACA 1 cut(s) 1050
Bsp143I GATC 4 cut(s) 249, 349, 369, 727
Bsp1720I GCTNAGC 1 cut(s) 477
Bsp19I CCATGG 1 cut(s) 492
BspANI GGCC 3 cut(s) 8, 966, 1065
BspCNI CTCAG 4 cut(s) 129, 319, 376, 469
BspFNI CGCG 1 cut(s) 105
BspLI GGNNCC 2 cut(s) 213, 214
BspMAI CTGCAG 1 cut(s) 1138
BspOI GCTAGC 1 cut(s) 668
BsrBI CCGCTC 1 cut(s) 481
BsrFI RCCGGY 1 cut(s) 156
BsrGI TGTACA 1 cut(s) 1050
BsrI ACTGG 2 cut(s) 534, 703
BssAI RCCGGY 1 cut(s) 156
BssECI CCNNGG 1 cut(s) 492
BssMI GATC 4 cut(s) 249, 349, 369, 727
BssNI GRCGYC 1 cut(s) 15
BssT1I CCWWGG 1 cut(s) 492
Bst2UI CCWGG 1 cut(s) 1067
Bst4CI ACNGT 6 cut(s) 198, 324, 503, 542, 691, 991
Bst6I CTCTTC 1 cut(s) 450
BstACI GRCGYC 1 cut(s) 15
BstAUI TGTACA 1 cut(s) 1050
BstC8I GCNNGC 5 cut(s) 98, 158, 575, 662, 666
BstDEI CTNAG 8 cut(s) 137, 306, 384, 416, 477, 656, 803, 1140
BstDSI CCRYGG 1 cut(s) 492
BstF5I GGATG 2 cut(s) 489, 1024
BstFNI CGCG 1 cut(s) 105
BstH2I RGCGCY 1 cut(s) 574
BstHHI GCGC 3 cut(s) 573, 595, 812
BstKTI GATC 4 cut(s) 252, 352, 372, 730
BstMAI GTCTC 1 cut(s) 804
BstMBI GATC 4 cut(s) 249, 349, 369, 727
BstMWI GCNNNNNNNGC 3 cut(s) 102, 157, 963
BstNI CCWGG 1 cut(s) 1067
BstSCI CCNGG 3 cut(s) 170, 588, 1065
BstSFI CTRYAG 1 cut(s) 1134
BstUI CGCG 1 cut(s) 105
BstV1I GCAGC 2 cut(s) 34, 628
BstV2I GAAGAC 1 cut(s) 510
BsuRI GGCC 3 cut(s) 8, 966, 1065
BtgI CCRYGG 1 cut(s) 492
BtsCI GGATG 2 cut(s) 489, 1024
BtsIMutI CAGTG 5 cut(s) 41, 134, 508, 541, 547
Cac8I GCNNGC 5 cut(s) 98, 158, 575, 662, 666
CfoI GCGC 3 cut(s) 573, 595, 812
Cfr10I RCCGGY 1 cut(s) 156
Cfr13I GGNCC 4 cut(s) 212, 320, 965, 1123
CseI GACGC 1 cut(s) 4
Csp6I GTAC 3 cut(s) 78, 759, 1051
CviAII CATG 4 cut(s) 217, 493, 565, 850
CviQI GTAC 3 cut(s) 78, 759, 1051
DdeI CTNAG 8 cut(s) 137, 306, 384, 416, 477, 656, 803, 1140
DpnI GATC 4 cut(s) 251, 351, 371, 729
DpnII GATC 4 cut(s) 249, 349, 369, 727
DraI TTTAAA 1 cut(s) 165
Eam1104I CTCTTC 1 cut(s) 450
EarI CTCTTC 1 cut(s) 450
EciI GGCGGA 2 cut(s) 55, 894
Eco130I CCWWGG 1 cut(s) 492
Eco147I AGGCCT 1 cut(s) 8
Eco47I GGWCC 3 cut(s) 212, 320, 1123
Eco57I CTGAAG 1 cut(s) 341
EcoO109I RGGNCCY 1 cut(s) 212
EcoRI GAATTC 1 cut(s) 896
EcoRII CCWGG 1 cut(s) 1065
EcoT14I CCWWGG 1 cut(s) 492
ErhI CCWWGG 1 cut(s) 492
FaeI CATG 4 cut(s) 220, 496, 568, 853
FaiI YATR 7 cut(s) 218, 230, 494, 566, 653, 851, 947
FaqI GGGAC 3 cut(s) 198, 572, 1118
FatI CATG 4 cut(s) 216, 492, 564, 849
FauI CCCGC 2 cut(s) 32, 202
FblI GTMKAC 1 cut(s) 561
Fnu4HI GCNGC 4 cut(s) 48, 297, 617, 1137
FokI GGATG 2 cut(s) 496, 1031
Fsp4HI GCNGC 4 cut(s) 48, 297, 617, 1137
FspBI CTAG 2 cut(s) 665, 756
GlaI GCGC 3 cut(s) 572, 594, 811
GluI GCNGC 4 cut(s) 48, 297, 617, 1137
HaeII RGCGCY 1 cut(s) 574
HaeIII GGCC 3 cut(s) 8, 966, 1065
HapII CCGG 4 cut(s) 12, 157, 171, 590
HgaI GACGC 1 cut(s) 4
HhaI GCGC 3 cut(s) 573, 595, 812
Hin1I GRCGYC 1 cut(s) 15
Hin1II CATG 4 cut(s) 220, 496, 568, 853
Hin6I GCGC 3 cut(s) 571, 593, 810
HinP1I GCGC 3 cut(s) 571, 593, 810
HincII GTYRAC 2 cut(s) 499, 648
HindII GTYRAC 2 cut(s) 499, 648
HindIII AAGCTT 1 cut(s) 98
HinfI GANTC 7 cut(s) 329, 506, 530, 797, 1000, 1022, 1042
HpaII CCGG 4 cut(s) 12, 157, 171, 590
HphI GGTGA 2 cut(s) 274, 929
Hpy166II GTNNAC 4 cut(s) 499, 562, 648, 719
Hpy188I TCNGA 6 cut(s) 309, 376, 529, 821, 1009, 1038
Hpy188III TCNNGA 7 cut(s) 119, 253, 335, 632, 677, 873, 901
Hpy8I GTNNAC 4 cut(s) 499, 562, 648, 719
Hpy99I CGWCG 7 cut(s) 20, 85, 88, 120, 185, 188, 1042
HpyAV CCTTC 9 cut(s) 226, 232, 238, 253, 439, 454, 629, 914, 1004
HpyCH4III ACNGT 6 cut(s) 198, 324, 503, 542, 691, 991
HpyCH4IV ACGT 1 cut(s) 80
HpyCH4V TGCA 8 cut(s) 96, 577, 616, 849, 864, 939, 957, 1136
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 157, 963
HpyF3I CTNAG 8 cut(s) 137, 306, 384, 416, 477, 656, 803, 1140
HpySE526I ACGT 1 cut(s) 80
Hsp92I GRCGYC 1 cut(s) 15
Hsp92II CATG 4 cut(s) 220, 496, 568, 853
HspAI GCGC 3 cut(s) 571, 593, 810
KflI GGGWCCC 1 cut(s) 212
KroI GCCGGC 1 cut(s) 156
KroNI GCCGGC 1 cut(s) 158
Kzo9I GATC 4 cut(s) 249, 349, 369, 727
LmnI GCTCC 2 cut(s) 881, 923
Lsp1109I GCAGC 2 cut(s) 34, 628
MaeI CTAG 2 cut(s) 665, 756
MaeII ACGT 1 cut(s) 80
MaeIII GTNAC 1 cut(s) 536
MalI GATC 4 cut(s) 251, 351, 371, 729
MbiI CCGCTC 1 cut(s) 481
MboI GATC 4 cut(s) 249, 349, 369, 727
MfeI CAATTG 2 cut(s) 404, 600
MluCI AATT 8 cut(s) 166, 389, 404, 434, 600, 896, 1093, 1098
MlyI GAGTC 2 cut(s) 806, 1016
MmeI TCCRAC 4 cut(s) 306, 799, 870, 1061
MroNI GCCGGC 1 cut(s) 156
MseI TTAA 5 cut(s) 90, 164, 471, 792, 1097
MslI CAYNNNNRTG 1 cut(s) 689
MspA1I CMGCKG 1 cut(s) 47
MspI CCGG 4 cut(s) 12, 157, 171, 590
MspR9I CCNGG 3 cut(s) 172, 590, 1067
MunI CAATTG 2 cut(s) 404, 600
Mva1269I GAATGC 1 cut(s) 746
MvaI CCWGG 1 cut(s) 1067
MvnI CGCG 1 cut(s) 105
MwoI GCNNNNNNNGC 3 cut(s) 102, 157, 963
NaeI GCCGGC 1 cut(s) 158
NciI CCSGG 2 cut(s) 172, 590
NcoI CCATGG 1 cut(s) 492
NdeII GATC 4 cut(s) 249, 349, 369, 727
NgoMIV GCCGGC 1 cut(s) 156
NheI GCTAGC 1 cut(s) 664
NlaIII CATG 4 cut(s) 220, 496, 568, 853
NlaIV GGNNCC 2 cut(s) 213, 214
NmuCI GTSAC 1 cut(s) 536
OliI CACNNNNGTG 1 cut(s) 689
PceI AGGCCT 1 cut(s) 8
PcsI WCGNNNNNNNCGW 1 cut(s) 521
PctI GAATGC 1 cut(s) 746
PdiI GCCGGC 1 cut(s) 158
PfeI GAWTC 5 cut(s) 329, 506, 530, 1000, 1042
PkrI GCNGC 4 cut(s) 49, 298, 618, 1138
PleI GAGTC 2 cut(s) 805, 1016
PpsI GAGTC 2 cut(s) 805, 1016
PpuMI RGGWCCY 1 cut(s) 212
Psp5II RGGWCCY 1 cut(s) 212
Psp6I CCWGG 1 cut(s) 1065
PspGI CCWGG 1 cut(s) 1065
PspN4I GGNNCC 2 cut(s) 213, 214
PspPI GGNCC 4 cut(s) 212, 320, 965, 1123
PspPPI RGGWCCY 1 cut(s) 212
PstI CTGCAG 1 cut(s) 1138
RsaI GTAC 3 cut(s) 79, 760, 1052
RsaNI GTAC 3 cut(s) 78, 759, 1051
RseI CAYNNNNRTG 1 cut(s) 689
SaqAI TTAA 5 cut(s) 90, 164, 471, 792, 1097
SatI GCNGC 4 cut(s) 48, 297, 617, 1137
Sau3AI GATC 4 cut(s) 249, 349, 369, 727
Sau96I GGNCC 4 cut(s) 212, 320, 965, 1123
ScaI AGTACT 1 cut(s) 760
SchI GAGTC 2 cut(s) 806, 1016
ScrFI CCNGG 3 cut(s) 172, 590, 1067
SfcI CTRYAG 1 cut(s) 1134
SinI GGWCC 3 cut(s) 212, 320, 1123
SmiI ATTTAAAT 1 cut(s) 165
SmiMI CAYNNNNRTG 1 cut(s) 689
SmlI CTYRAG 2 cut(s) 272, 333
SmoI CTYRAG 2 cut(s) 272, 333
SpeI ACTAGT 1 cut(s) 755
Sse9I AATT 8 cut(s) 166, 389, 404, 434, 600, 896, 1093, 1098
SseBI AGGCCT 1 cut(s) 8
SspMI CTAG 2 cut(s) 665, 756
StuI AGGCCT 1 cut(s) 8
StyD4I CCNGG 3 cut(s) 170, 588, 1065
StyI CCWWGG 1 cut(s) 492
SwaI ATTTAAAT 1 cut(s) 165
TaaI ACNGT 6 cut(s) 198, 324, 503, 542, 691, 991
TaiI ACGT 1 cut(s) 83
TaqI TCGA 4 cut(s) 57, 284, 348, 900
TasI AATT 8 cut(s) 166, 389, 404, 434, 600, 896, 1093, 1098
TatI WGTACW 2 cut(s) 758, 1050
TauI GCSGC 1 cut(s) 299
TfiI GAWTC 5 cut(s) 329, 506, 530, 1000, 1042
Tru1I TTAA 5 cut(s) 90, 164, 471, 792, 1097
Tru9I TTAA 5 cut(s) 90, 164, 471, 792, 1097
TscAI CASTG 5 cut(s) 41, 141, 508, 541, 547
TseFI GTSAC 1 cut(s) 536
TseI GCWGC 3 cut(s) 47, 616, 1136
Tsp45I GTSAC 1 cut(s) 536
TspDTI ATGAA 4 cut(s) 245, 500, 510, 581
TspGWI ACGGA 2 cut(s) 201, 1015
TspRI CASTG 5 cut(s) 41, 141, 508, 541, 547
VpaK11BI GGWCC 3 cut(s) 212, 320, 1123
XapI RAATTY 2 cut(s) 166, 896
XmiI GTMKAC 1 cut(s) 561
XspI CTAG 2 cut(s) 665, 756
ZrmI AGTACT 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.