Rorug04G0220200

Protein CHUP1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000826
Physical Location & Seq
Reverse (-)
37989725 .. 37994102
4378 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0220200.1

Sequence Viewer

Length: 978 bp
ATGGCAGTGGAGAAGGCTGGTATAGCTAAAGATGTTACTGAATTGATTGGGAAAACCCCATTGGTGTATCTGAACCATATTGTGGAGGGTTGTGTTGCTCGTATCGCTGCCAAATTGGAAATGATGGAGCCCTGCTCTAGTGTAAAAGACAGAATTGGTTATAGTATGATTGCAGATGCTGAGGCAAAGGGCCTTATCACACCTGGGCAGAGTGTCCTAATTGAGCCAACAAGTGGTAACACTGGCATAGGGCTAGCTTTCATGGCGGCAGCTAAACAGTACAGGCTTATCATTACAATGCCTGCTTCAATGAGTCTTGAAAGAAGAATCATCCTCCGAGCCTTCGGAGCTGAGTTGGTTCTCACAGACCCTGCAAAGGGCATGAAAGGGGCTGTTCAGAAGGCTGAGGAGATATTGGCCAAGACACCTAATGCTTACATGCTCCAGCAGTTTGAGAATCCTGCCAACCCAAAGGTACATTATGAAACCACTGGACCGGAGATATGGGAAGGCACTGGAGGGAAAGTAGATGCTTTTGTTTCTGGGATAGGTACAGGTGGAACTATAACTGGTGCAGGAAAATATCTTAAAGAGAAGAACCCTAGTGTAAAGCTGTATGGTGTGGAACCAGTTGAGAGTCCAGTCCTAACCGGAGGAAAGCCTGGTCCACACAAGATCCAAGGAATAGGTGCTGGTTTTGTCCCAGGTGTCTTGGAAGTCAATATCATTGATGAAGTCATTCAAATATCAAGTGATGAAGCAATTGAAACTGCCAAGCTTCTTGCAACAAAAGAAGGTTTGCTTGTGGGAATATCATCTGGTGCTGCTGCCGCTGCTGCAGTTAGGATTGCAAAGAGACCAGAGAATGCTGGAAAGCTCATTGTTGTGGTTTTCCCAAGCTTTGGGGAGAGATATCTCTCCTCTGTGCTGTTTGAGTCAGTGAGACGGGAAGCTGAAAGCATGACCTTTGAGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

325

Amino Acids

34.3

Weight (kDa)

6.07

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 12 - 299 7.3e-76 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 82, 233, 902
AciI CCGC 2 cut(s) 266, 831
AclWI GGATC 1 cut(s) 670
AcoI YGGCCR 1 cut(s) 417
AfaI GTAC 3 cut(s) 281, 477, 553
AfiI CCNNNNNNNGG 5 cut(s) 82, 233, 376, 377, 902
AgsI TTSAA 4 cut(s) 309, 320, 743, 767
AjnI CCWGG 3 cut(s) 202, 661, 703
AjuI GAANNNNNNNTTGG 2 cut(s) 44, 76
AluBI AGCT 9 cut(s) 26, 257, 272, 350, 613, 778, 877, 900, 953
AluI AGCT 9 cut(s) 26, 257, 272, 350, 613, 778, 877, 900, 953
Alw26I GTCTC 3 cut(s) 850, 937, 965
AlwI GGATC 1 cut(s) 670
AlwNI CAGNNNCTG 2 cut(s) 179, 371
AoxI GGCC 2 cut(s) 190, 417
ApeKI GCWGC 6 cut(s) 107, 269, 824, 827, 833, 836
Asp700I GAANNNNTTC 1 cut(s) 738
AspS9I GGNCC 3 cut(s) 190, 494, 665
AsuNHI GCTAGC 1 cut(s) 253
AvaII GGWCC 2 cut(s) 494, 665
BalI TGGCCA 1 cut(s) 419
BanII GRGCYC 1 cut(s) 132
BbvCI CCTCAGC 2 cut(s) 180, 405
BbvI GCAGC 6 cut(s) 94, 281, 811, 814, 820, 823
BccI CCATC 1 cut(s) 118
BciT130I CCWGG 3 cut(s) 204, 663, 705
BcoDI GTCTC 3 cut(s) 850, 937, 965
BfaI CTAG 3 cut(s) 138, 254, 603
BfmI CTRYAG 1 cut(s) 837
BisI GCNGC 8 cut(s) 108, 267, 270, 825, 828, 831, 834, 837
BlsI GCNGC 8 cut(s) 109, 268, 271, 826, 829, 832, 835, 838
Bme1390I CCNGG 3 cut(s) 204, 663, 705
Bme18I GGWCC 2 cut(s) 494, 665
BmgT120I GGNCC 3 cut(s) 190, 494, 665
BmiI GGNNCC 2 cut(s) 129, 627
BmrFI CCNGG 3 cut(s) 204, 663, 705
BmsI GCATC 2 cut(s) 166, 520
BmtI GCTAGC 1 cut(s) 257
BplI GAGNNNNNCTC 2 cut(s) 119, 151
BpmI CTGGAG 2 cut(s) 428, 537
Bpu10I CCTNAGC 2 cut(s) 180, 405
BsaI GGTCTC 2 cut(s) 850, 965
BsaJI CCNNGG 3 cut(s) 203, 679, 703
BsaWI WCCGGW 2 cut(s) 496, 650
BsaXI ACNNNNNCTCC 2 cut(s) 510, 540
Bsc4I CCNNNNNNNGG 5 cut(s) 82, 233, 376, 377, 902
Bse1I ACTGG 6 cut(s) 247, 496, 520, 574, 629, 641
BseBI CCWGG 3 cut(s) 204, 663, 705
BseDI CCNNGG 3 cut(s) 203, 679, 703
BseGI GGATG 1 cut(s) 330
BseLI CCNNNNNNNGG 5 cut(s) 82, 233, 376, 377, 902
BseMII CTCAG 3 cut(s) 171, 342, 396
BseNI ACTGG 6 cut(s) 247, 496, 520, 574, 629, 641
BseRI GAGGAG 2 cut(s) 422, 910
BseXI GCAGC 6 cut(s) 94, 281, 811, 814, 820, 823
BsgI GTGCAG 1 cut(s) 594
BshFI GGCC 2 cut(s) 192, 419
BsiSI CCGG 2 cut(s) 497, 651
BslFI GGGAC 1 cut(s) 686
BslI CCNNNNNNNGG 5 cut(s) 82, 233, 376, 377, 902
BsmAI GTCTC 3 cut(s) 850, 937, 965
BsmBI CGTCTC 1 cut(s) 937
BsmFI GGGAC 1 cut(s) 686
BsmI GAATGC 1 cut(s) 871
BsnI GGCC 2 cut(s) 192, 419
Bso31I GGTCTC 2 cut(s) 850, 965
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 1 cut(s) 675
BspACI CCGC 2 cut(s) 266, 831
BspANI GGCC 2 cut(s) 192, 419
BspCNI CTCAG 3 cut(s) 172, 343, 397
BspLI GGNNCC 2 cut(s) 129, 627
BspMAI CTGCAG 1 cut(s) 841
BspOI GCTAGC 1 cut(s) 257
BspPI GGATC 1 cut(s) 670
BspTNI GGTCTC 2 cut(s) 850, 965
BsrI ACTGG 6 cut(s) 247, 496, 520, 574, 629, 641
BssECI CCNNGG 3 cut(s) 203, 679, 703
BssMI GATC 1 cut(s) 675
BssT1I CCWWGG 1 cut(s) 679
Bst2UI CCWGG 3 cut(s) 204, 663, 705
Bst4CI ACNGT 1 cut(s) 279
BstC8I GCNNGC 2 cut(s) 255, 303
BstDEI CTNAG 3 cut(s) 180, 351, 405
BstF5I GGATG 1 cut(s) 330
BstKTI GATC 1 cut(s) 678
BstMAI GTCTC 3 cut(s) 850, 937, 965
BstMBI GATC 1 cut(s) 675
BstMWI GCNNNNNNNGC 7 cut(s) 23, 104, 263, 347, 830, 833, 836
BstNI CCWGG 3 cut(s) 204, 663, 705
BstNSI RCATGY 1 cut(s) 442
BstSCI CCNGG 3 cut(s) 202, 661, 703
BstSFI CTRYAG 1 cut(s) 837
BstV1I GCAGC 6 cut(s) 94, 281, 811, 814, 820, 823
BstX2I RGATCY 1 cut(s) 675
BstYI RGATCY 1 cut(s) 675
BsuRI GGCC 2 cut(s) 192, 419
BtsCI GGATG 1 cut(s) 330
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 5 cut(s) 12, 240, 489, 513, 945
Cac8I GCNNGC 2 cut(s) 255, 303
CaiI CAGNNNCTG 2 cut(s) 179, 371
Cfr13I GGNCC 3 cut(s) 190, 494, 665
Csp6I GTAC 3 cut(s) 280, 476, 552
CviAII CATG 4 cut(s) 262, 382, 439, 961
CviQI GTAC 3 cut(s) 280, 476, 552
DdeI CTNAG 3 cut(s) 180, 351, 405
DpnI GATC 1 cut(s) 677
DpnII GATC 1 cut(s) 675
EaeI YGGCCR 1 cut(s) 417
Eco130I CCWWGG 1 cut(s) 679
Eco24I GRGCYC 1 cut(s) 132
Eco31I GGTCTC 2 cut(s) 850, 965
Eco32I GATATC 1 cut(s) 914
Eco47I GGWCC 2 cut(s) 494, 665
EcoO109I RGGNCCY 1 cut(s) 190
EcoRII CCWGG 3 cut(s) 202, 661, 703
EcoRV GATATC 1 cut(s) 914
EcoT14I CCWWGG 1 cut(s) 679
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 679
Esp3I CGTCTC 1 cut(s) 937
FaeI CATG 4 cut(s) 265, 385, 442, 964
FalI AAGNNNNNCTT 2 cut(s) 786, 818
FaqI GGGAC 1 cut(s) 686
FatI CATG 4 cut(s) 261, 381, 438, 960
Fnu4HI GCNGC 8 cut(s) 108, 267, 270, 825, 828, 831, 834, 837
FokI GGATG 1 cut(s) 317
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 8 cut(s) 108, 267, 270, 825, 828, 831, 834, 837
FspBI CTAG 3 cut(s) 138, 254, 603
GluI GCNGC 8 cut(s) 108, 267, 270, 825, 828, 831, 834, 837
GsuI CTGGAG 2 cut(s) 428, 537
HaeIII GGCC 2 cut(s) 192, 419
HapII CCGG 2 cut(s) 497, 651
Hin1II CATG 4 cut(s) 265, 385, 442, 964
HindIII AAGCTT 2 cut(s) 776, 898
HinfI GANTC 5 cut(s) 313, 327, 457, 637, 935
HpaII CCGG 2 cut(s) 497, 651
Hpy166II GTNNAC 1 cut(s) 668
Hpy188I TCNGA 4 cut(s) 72, 338, 347, 399
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 1 cut(s) 668
HpyAV CCTTC 5 cut(s) 7, 352, 394, 503, 788
HpyCH4III ACNGT 1 cut(s) 279
HpyCH4V TGCA 6 cut(s) 173, 374, 575, 785, 839, 851
HpyF10VI GCNNNNNNNGC 7 cut(s) 23, 104, 263, 347, 830, 833, 836
HpyF3I CTNAG 3 cut(s) 180, 351, 405
Hsp92II CATG 4 cut(s) 265, 385, 442, 964
Kzo9I GATC 1 cut(s) 675
LmnI GCTCC 3 cut(s) 127, 347, 447
Lsp1109I GCAGC 6 cut(s) 94, 281, 811, 814, 820, 823
LweI GCATC 2 cut(s) 166, 520
MaeI CTAG 3 cut(s) 138, 254, 603
MaeIII GTNAC 2 cut(s) 34, 236
MalI GATC 1 cut(s) 677
MboI GATC 1 cut(s) 675
MboII GAAGA 2 cut(s) 336, 607
MfeI CAATTG 1 cut(s) 762
MflI RGATCY 1 cut(s) 675
MhlI GDGCHC 1 cut(s) 132
MlsI TGGCCA 1 cut(s) 419
MluCI AATT 5 cut(s) 41, 113, 153, 219, 762
MluNI TGGCCA 1 cut(s) 419
MlyI GAGTC 3 cut(s) 322, 646, 944
MnlI CCTC 7 cut(s) 79, 175, 344, 400, 512, 647, 931
Mox20I TGGCCA 1 cut(s) 419
MroXI GAANNNNTTC 1 cut(s) 738
MscI TGGCCA 1 cut(s) 419
MseI TTAA 1 cut(s) 588
MslI CAYNNNNRTG 2 cut(s) 296, 884
Msp20I TGGCCA 1 cut(s) 419
MspA1I CMGCKG 1 cut(s) 833
MspI CCGG 2 cut(s) 497, 651
MspR9I CCNGG 3 cut(s) 204, 663, 705
MunI CAATTG 1 cut(s) 762
Mva1269I GAATGC 1 cut(s) 871
MvaI CCWGG 3 cut(s) 204, 663, 705
MwoI GCNNNNNNNGC 7 cut(s) 23, 104, 263, 347, 830, 833, 836
NdeII GATC 1 cut(s) 675
NheI GCTAGC 1 cut(s) 253
NlaIII CATG 4 cut(s) 265, 385, 442, 964
NlaIV GGNNCC 2 cut(s) 129, 627
NspI RCATGY 1 cut(s) 442
PctI GAATGC 1 cut(s) 871
PdmI GAANNNNTTC 1 cut(s) 738
PfeI GAWTC 2 cut(s) 327, 457
PflMI CCANNNNNTGG 3 cut(s) 82, 233, 902
PkrI GCNGC 8 cut(s) 109, 268, 271, 826, 829, 832, 835, 838
PleI GAGTC 3 cut(s) 321, 645, 943
PpsI GAGTC 3 cut(s) 321, 645, 943
Psp6I CCWGG 3 cut(s) 202, 661, 703
PspGI CCWGG 3 cut(s) 202, 661, 703
PspN4I GGNNCC 2 cut(s) 129, 627
PspPI GGNCC 3 cut(s) 190, 494, 665
PstI CTGCAG 1 cut(s) 841
PstNI CAGNNNCTG 2 cut(s) 179, 371
PsuI RGATCY 1 cut(s) 675
RsaI GTAC 3 cut(s) 281, 477, 553
RsaNI GTAC 3 cut(s) 280, 476, 552
RseI CAYNNNNRTG 2 cut(s) 296, 884
SaqAI TTAA 1 cut(s) 588
SatI GCNGC 8 cut(s) 108, 267, 270, 825, 828, 831, 834, 837
Sau3AI GATC 1 cut(s) 675
Sau96I GGNCC 3 cut(s) 190, 494, 665
SchI GAGTC 3 cut(s) 322, 646, 944
ScrFI CCNGG 3 cut(s) 204, 663, 705
SduI GDGCHC 1 cut(s) 132
SfaNI GCATC 2 cut(s) 166, 520
SfcI CTRYAG 1 cut(s) 837
SinI GGWCC 2 cut(s) 494, 665
SmiMI CAYNNNNRTG 2 cut(s) 296, 884
Sse9I AATT 5 cut(s) 41, 113, 153, 219, 762
SsiI CCGC 2 cut(s) 266, 831
SspMI CTAG 3 cut(s) 138, 254, 603
StyD4I CCNGG 3 cut(s) 202, 661, 703
StyI CCWWGG 1 cut(s) 679
TaaI ACNGT 1 cut(s) 279
TasI AATT 5 cut(s) 41, 113, 153, 219, 762
TatI WGTACW 1 cut(s) 279
TauI GCSGC 2 cut(s) 269, 833
TfiI GAWTC 2 cut(s) 327, 457
Tru1I TTAA 1 cut(s) 588
Tru9I TTAA 1 cut(s) 588
TscAI CASTG 5 cut(s) 12, 247, 496, 520, 945
TseI GCWGC 6 cut(s) 107, 269, 824, 827, 833, 836
TspDTI ATGAA 5 cut(s) 250, 398, 498, 747, 771
TspRI CASTG 5 cut(s) 12, 247, 496, 520, 945
Van91I CCANNNNNTGG 3 cut(s) 82, 233, 902
VpaK11BI GGWCC 2 cut(s) 494, 665
XceI RCATGY 1 cut(s) 442
XmnI GAANNNNTTC 1 cut(s) 738
XspI CTAG 3 cut(s) 138, 254, 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.