AT5G10880

tRNA synthetase class II (G, H, P and S) family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000826
Physical Location & Seq
Forward (+)
3432693 .. 3433867
1175 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G10880.1

Sequence Viewer

Length: 930 bp
ATGGTGAAAGAAACTCGTCTTGGAATCACTGCAAAGAAAGATGAAGATTTTGGGAAGTGGTATTCCGAGGCTTGTAGATTTGGTGAATTGGTTGAATACTACGAGTCTGTAAAGGGATGCTACATTCTCAAGCCATCAGGCATCAGCGATGAATATTTGGAACGTTATACGAACACCACACTCAAGGGTTTGCTCCAGAGGTTGCTTGGGTTACAAGAGCTGGTGGATAAGAGGACACAGGGACCTCCCTTTGAAGCCGCGTGTATGACTCATGGAGATGACAAAGGTTTAGTATTCCCTCCTAAAGTGGCACCTGTTCAAGTTGTTGTAATCCATGTTCCCATCAAAGGCGCTGCTGATTATCAAGAACTTTGTGATGCTTGTGAAGCTGTTGAATCCACCTTGCTTGGGGCTGGAATCCGTGCTGAAGCAGATATACGCGACAACTATTCTTGCGGATGGAAGTATGCGGATCAAGAACTAACGGGTGTTCCTTTGAGAATTGAAACTGGACCTAGAGATTTGGCCAATGATCAGGTGAGGATAGTGACGCGTGACAATGGAGCTAAGATGGATGTCAAGAGAGGGGATTTGATTGAACAAGTTAAGGACTTGCTAGAGAAAATCCAGAGTAACCTCTATGATGTGGCAAAGAGAAAGGTTGAAGAATGTACTCAAAAGGTTGAAACTTGGGATGAATTTGTGGAAGCTTTGAGCCAAAAGAAACTGATTTTAGCTCCATGGTGTGATAAGGTTGAAGTGGAGAAGGATGTGAAGAGGCGTACCAGAGGTGATGAAACAGGAGGAGGAGGAGGAGGAGCTAAGACTCTATGTACTCCTCTTGAGCAGCCTGAACTAGGAGAAGAGACTCTATGCTTTGCATCAGGAAAGCCAGCCAAGAAGTGGAGCTACTGGGGAAGAAGTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
HGTP_anticodon ProRS-C_1 tRNA-synt_2b

Protein Analysis

309

Amino Acids

34.58

Weight (kDa)

5.26

Isoelectric Point (pI)

42.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HGTP_anticodon PF03129 107 - 204 5e-18 Anticodon binding domain
ProRS-C_1 PF09180 231 - 309 6.1e-24 Prolyl-tRNA synthetase, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 310
AccB7I CCANNNNNTGG 1 cut(s) 903
AccII CGCG 3 cut(s) 260, 441, 553
AciI CCGC 3 cut(s) 258, 456, 470
AclI AACGTT 1 cut(s) 163
AclWI GGATC 1 cut(s) 480
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 1 cut(s) 698
AcuI CTGAAG 1 cut(s) 447
AfaI GTAC 3 cut(s) 673, 784, 835
AfiI CCNNNNNNNGG 4 cut(s) 347, 408, 857, 903
AflIII ACRYGT 1 cut(s) 551
AgsI TTSAA 9 cut(s) 95, 254, 320, 395, 506, 599, 665, 686, 758
AjuI GAANNNNNNNTTGG 1 cut(s) 35
AluBI AGCT 7 cut(s) 220, 389, 566, 710, 737, 821, 909
AluI AGCT 7 cut(s) 220, 389, 566, 710, 737, 821, 909
Alw26I GTCTC 1 cut(s) 860
AlwI GGATC 1 cut(s) 480
AoxI GGCC 1 cut(s) 525
ApeKI GCWGC 2 cut(s) 353, 847
ApoI RAATTY 1 cut(s) 698
AspLEI GCGC 1 cut(s) 353
AspS9I GGNCC 2 cut(s) 242, 512
AsuHPI GGTGA 4 cut(s) 16, 95, 550, 803
AvaII GGWCC 2 cut(s) 242, 512
BalI TGGCCA 1 cut(s) 527
BanI GGYRCC 1 cut(s) 310
BarI GAAGNNNNNNTAC 4 cut(s) 420, 452, 893, 925
BbvI GCAGC 2 cut(s) 340, 859
BccI CCATC 4 cut(s) 142, 350, 453, 565
BclI TGATCA 1 cut(s) 532
BcoDI GTCTC 1 cut(s) 860
BfaI CTAG 3 cut(s) 516, 617, 857
BfoI RGCGCY 1 cut(s) 354
BisI GCNGC 3 cut(s) 258, 354, 848
BlsI GCNGC 3 cut(s) 259, 355, 849
Bme18I GGWCC 2 cut(s) 242, 512
BmgT120I GGNCC 2 cut(s) 242, 512
BmiI GGNNCC 2 cut(s) 243, 312
BmrI ACTGGG 1 cut(s) 922
BmsI GCATC 4 cut(s) 107, 150, 367, 890
BmuI ACTGGG 1 cut(s) 922
BpmI CTGGAG 1 cut(s) 179
BpuEI CTTGAG 3 cut(s) 113, 167, 863
BsaJI CCNNGG 2 cut(s) 66, 740
Bsc4I CCNNNNNNNGG 4 cut(s) 347, 408, 857, 903
Bse1I ACTGG 2 cut(s) 514, 917
BseDI CCNNGG 2 cut(s) 66, 740
BseGI GGATG 5 cut(s) 122, 464, 580, 700, 775
BseLI CCNNNNNNNGG 4 cut(s) 347, 408, 857, 903
BseNI ACTGG 2 cut(s) 514, 917
BseRI GAGGAG 6 cut(s) 819, 822, 825, 828, 828, 831
BseXI GCAGC 2 cut(s) 340, 859
Bsh1236I CGCG 3 cut(s) 260, 441, 553
BshFI GGCC 1 cut(s) 527
BshNI GGYRCC 1 cut(s) 310
BslFI GGGAC 1 cut(s) 255
BslI CCNNNNNNNGG 4 cut(s) 347, 408, 857, 903
BsmAI GTCTC 1 cut(s) 860
BsmFI GGGAC 1 cut(s) 255
BsnI GGCC 1 cut(s) 527
Bsp143I GATC 2 cut(s) 472, 532
Bsp19I CCATGG 1 cut(s) 740
BspACI CCGC 3 cut(s) 258, 456, 470
BspANI GGCC 1 cut(s) 527
BspFNI CGCG 3 cut(s) 260, 441, 553
BspLI GGNNCC 2 cut(s) 243, 312
BspPI GGATC 1 cut(s) 480
BspT107I GGYRCC 1 cut(s) 310
BsrI ACTGG 2 cut(s) 514, 917
BssECI CCNNGG 2 cut(s) 66, 740
BssMI GATC 2 cut(s) 472, 532
BssT1I CCWWGG 1 cut(s) 740
Bst6I CTCTTC 2 cut(s) 770, 858
BstC8I GCNNGC 1 cut(s) 894
BstDEI CTNAG 2 cut(s) 567, 822
BstDSI CCRYGG 1 cut(s) 740
BstENI CCTNNNNNAGG 1 cut(s) 855
BstF5I GGATG 5 cut(s) 122, 464, 580, 700, 775
BstFNI CGCG 3 cut(s) 260, 441, 553
BstH2I RGCGCY 1 cut(s) 354
BstHHI GCGC 1 cut(s) 353
BstKTI GATC 2 cut(s) 475, 535
BstMAI GTCTC 1 cut(s) 860
BstMBI GATC 2 cut(s) 472, 532
BstMWI GCNNNNNNNGC 1 cut(s) 386
BstUI CGCG 3 cut(s) 260, 441, 553
BstV1I GCAGC 2 cut(s) 340, 859
BsuRI GGCC 1 cut(s) 527
BtgI CCRYGG 1 cut(s) 740
BtgZI GCGATG 1 cut(s) 162
BtsCI GGATG 5 cut(s) 122, 464, 580, 700, 775
BtsI GCAGTG 1 cut(s) 27
BtsIMutI CAGTG 1 cut(s) 27
Cac8I GCNNGC 1 cut(s) 894
CfoI GCGC 1 cut(s) 353
Cfr13I GGNCC 2 cut(s) 242, 512
CseI GACGC 1 cut(s) 559
Csp6I GTAC 3 cut(s) 672, 783, 834
CspCI CAANNNNNGTGG 2 cut(s) 388, 423
CviAII CATG 3 cut(s) 272, 335, 741
CviQI GTAC 3 cut(s) 672, 783, 834
DdeI CTNAG 2 cut(s) 567, 822
DpnI GATC 2 cut(s) 474, 534
DpnII GATC 2 cut(s) 472, 532
EaeI YGGCCR 1 cut(s) 525
Eam1104I CTCTTC 2 cut(s) 770, 858
EarI CTCTTC 2 cut(s) 770, 858
Eco130I CCWWGG 1 cut(s) 740
Eco47I GGWCC 2 cut(s) 242, 512
Eco57I CTGAAG 1 cut(s) 447
EcoNI CCTNNNNNAGG 1 cut(s) 855
EcoO109I RGGNCCY 1 cut(s) 242
EcoT14I CCWWGG 1 cut(s) 740
ErhI CCWWGG 1 cut(s) 740
FaeI CATG 3 cut(s) 275, 338, 744
FaqI GGGAC 1 cut(s) 255
FatI CATG 3 cut(s) 271, 334, 740
FbaI TGATCA 1 cut(s) 532
Fnu4HI GCNGC 3 cut(s) 258, 354, 848
FokI GGATG 5 cut(s) 129, 471, 587, 707, 782
Fsp4HI GCNGC 3 cut(s) 258, 354, 848
FspBI CTAG 3 cut(s) 516, 617, 857
GlaI GCGC 1 cut(s) 352
GluI GCNGC 3 cut(s) 258, 354, 848
GsuI CTGGAG 1 cut(s) 179
HaeII RGCGCY 1 cut(s) 354
HaeIII GGCC 1 cut(s) 527
HgaI GACGC 1 cut(s) 559
HhaI GCGC 1 cut(s) 353
Hin1II CATG 3 cut(s) 275, 338, 744
Hin6I GCGC 1 cut(s) 351
HinP1I GCGC 1 cut(s) 351
HindIII AAGCTT 1 cut(s) 708
HinfI GANTC 7 cut(s) 24, 104, 268, 395, 417, 826, 868
HphI GGTGA 4 cut(s) 16, 95, 550, 803
Hpy188I TCNGA 1 cut(s) 67
Hpy188III TCNNGA 7 cut(s) 196, 365, 476, 580, 628, 842, 885
HpyAV CCTTC 1 cut(s) 760
HpyCH4IV ACGT 1 cut(s) 163
HpyCH4V TGCA 2 cut(s) 32, 881
HpyF10VI GCNNNNNNNGC 1 cut(s) 386
HpyF3I CTNAG 2 cut(s) 567, 822
HpySE526I ACGT 1 cut(s) 163
Hsp92II CATG 3 cut(s) 275, 338, 744
HspAI GCGC 1 cut(s) 351
Ksp22I TGATCA 1 cut(s) 532
Kzo9I GATC 2 cut(s) 472, 532
LmnI GCTCC 5 cut(s) 198, 563, 742, 818, 906
Lsp1109I GCAGC 2 cut(s) 340, 859
LweI GCATC 4 cut(s) 107, 150, 367, 890
MaeI CTAG 3 cut(s) 516, 617, 857
MaeII ACGT 1 cut(s) 163
MaeIII GTNAC 5 cut(s) 210, 547, 554, 632, 923
MalI GATC 2 cut(s) 474, 534
MboI GATC 2 cut(s) 472, 532
MboII GAAGA 5 cut(s) 56, 677, 787, 875, 930
MlsI TGGCCA 1 cut(s) 527
MluCI AATT 3 cut(s) 86, 501, 698
MluI ACGCGT 1 cut(s) 551
MluNI TGGCCA 1 cut(s) 527
MlyI GAGTC 4 cut(s) 113, 262, 820, 862
Mox20I TGGCCA 1 cut(s) 527
MscI TGGCCA 1 cut(s) 527
MseI TTAA 1 cut(s) 606
MslI CAYNNNNRTG 1 cut(s) 276
Msp20I TGGCCA 1 cut(s) 527
MvnI CGCG 3 cut(s) 260, 441, 553
MwoI GCNNNNNNNGC 1 cut(s) 386
NcoI CCATGG 1 cut(s) 740
NdeII GATC 2 cut(s) 472, 532
NlaIII CATG 3 cut(s) 275, 338, 744
NlaIV GGNNCC 2 cut(s) 243, 312
NmuCI GTSAC 2 cut(s) 547, 554
PfeI GAWTC 3 cut(s) 24, 395, 417
PflMI CCANNNNNTGG 1 cut(s) 903
PkrI GCNGC 3 cut(s) 259, 355, 849
PleI GAGTC 4 cut(s) 112, 262, 820, 862
PpsI GAGTC 4 cut(s) 112, 262, 820, 862
PpuMI RGGWCCY 1 cut(s) 242
Psp1406I AACGTT 1 cut(s) 163
Psp5II RGGWCCY 1 cut(s) 242
PspN4I GGNNCC 2 cut(s) 243, 312
PspPI GGNCC 2 cut(s) 242, 512
PspPPI RGGWCCY 1 cut(s) 242
PsrI GAACNNNNNNTAC 2 cut(s) 321, 353
RsaI GTAC 3 cut(s) 673, 784, 835
RsaNI GTAC 3 cut(s) 672, 783, 834
RseI CAYNNNNRTG 1 cut(s) 276
SaqAI TTAA 1 cut(s) 606
SatI GCNGC 3 cut(s) 258, 354, 848
Sau3AI GATC 2 cut(s) 472, 532
Sau96I GGNCC 2 cut(s) 242, 512
SchI GAGTC 4 cut(s) 113, 262, 820, 862
SfaNI GCATC 4 cut(s) 107, 150, 367, 890
SinI GGWCC 2 cut(s) 242, 512
SmiMI CAYNNNNRTG 1 cut(s) 276
SmlI CTYRAG 3 cut(s) 128, 182, 842
SmoI CTYRAG 3 cut(s) 128, 182, 842
Sse9I AATT 3 cut(s) 86, 501, 698
SsiI CCGC 3 cut(s) 258, 456, 470
SspI AATATT 1 cut(s) 155
SspMI CTAG 3 cut(s) 516, 617, 857
StyI CCWWGG 1 cut(s) 740
TaiI ACGT 1 cut(s) 166
TasI AATT 3 cut(s) 86, 501, 698
TatI WGTACW 2 cut(s) 671, 833
TauI GCSGC 1 cut(s) 260
TfiI GAWTC 3 cut(s) 24, 395, 417
Tru1I TTAA 1 cut(s) 606
Tru9I TTAA 1 cut(s) 606
TscAI CASTG 1 cut(s) 34
TseFI GTSAC 2 cut(s) 547, 554
TseI GCWGC 2 cut(s) 353, 847
Tsp45I GTSAC 2 cut(s) 547, 554
TspDTI ATGAA 4 cut(s) 57, 165, 711, 810
TspGWI ACGGA 1 cut(s) 410
TspRI CASTG 1 cut(s) 34
Van91I CCANNNNNTGG 1 cut(s) 903
VpaK11BI GGWCC 2 cut(s) 242, 512
XagI CCTNNNNNAGG 1 cut(s) 855
XapI RAATTY 1 cut(s) 698
XcmI CCANNNNNNNNNTGG 2 cut(s) 203, 900
XspI CTAG 3 cut(s) 516, 617, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.