Rh4AG277200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0013525
Physical Location & Seq
Forward (+)
59376511 .. 59378242
1732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG277200.1

Sequence Viewer

Length: 1071 bp
ATGGAGGTTTCTGGATTAGTTTCCCGCCAAGGTCCGTGCTTTTCCGGCGCCGGAATCGATACCCGGTCTGCAAATTCATCGTTTTCCGGCGACGTCCACTTTCCGGGTCGTAATGAATTTGGAGTGAGTTTGAGGACCCGAAGGGCTATGAGTTCTGGGTTTTGCGATAAGGGGCATGTGGAGTATTATTATTCGGGTCCCAGATGCGGTGGTAATAAGAAGGAGAAGGAGATCAAGAAGAAATTGAAGTTGCTTAAAGGATTGTCGGAGCTGTCTGCGTCTTCTCAGATTAGTACTGGGTTGGATTCCGAGGAGGGTTTGGCTGCTCAAGTTCAGGGGAAGCTGATTTCGGAAGGTGCAGAAGCATTGCTACAGCAATTGGAACAGGTAAGAGCTGAGGAGAAGGAGTTGAAGAAAAAGAAGAAAGAAGAGAAGGCGAGGCTTAAAGCAGAGCGGATGAAAACCATGAAGGACTGTGAATCGTCATCTTCATCGTCTTCATCTGAGTCGAGTGAGAGTGAATGTGGGGAGGTGGTTGACATGAACAGCCTGAGAGGTGAGGTCCCTGCGCAACCGATGCTAGAGGGTTTGCAGCCATCGACCCAAGGAGGGGTGGCATTATTGACCCAACCGAGTTCGGTAACCACACTTCAAGAAGATAAATGCTGCACTGGAGCTAGTACTAGTTGTGGCAGCAGTGATAGTATTGGTCCTAACAATGCGGCTAGTAGCTCAGTTATGGAAGCATCTGTTGTGACGAAGATTGAAGTATGCATGGGCAAAAAGTGCAAGACATCAGGAGGTGTAGCCTTGTTGGAAGAATTCGAGAGGTTGATGGGTGTCGAAGGAAGTGTTGTTGGGTGCAAGTGTATGGGAAAATGCAAAAATGGTCCAAATGTGAGGGTTTTGAATTCTCTGGATGAGATTGAATCCGAAGAAGGAACAGAGGACTCTGTAAGGTATCCCACTAATCCTTTGTACATTGGAGTGGGGCTGGAAGATGTGAGCCTCATCGTGGCCAACTTAATGGGGGAAGTCAAGAAGGATTTGGGGCTTGCCACTTCAGCTTAG

Protein Analysis

356

Amino Acids

37.72

Weight (kDa)

5.39

Isoelectric Point (pI)

48.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013525)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48300
fragaria_vesca FvH4_4g21650 FvH4_4g21660
malus_domestica MD13G1156700.v1.1 MD16G1134300.v1.1
prunus_persica Prupe.1G103600_v2.0.a1 Prupe.1G103600_v2.0.a1
pyrus_communis pycom13g12050 pycom16g11860
rosa_chinensis RchiOBHm_Chr4g0427881
rosa_multiflora Rmu_co8387153.1_g000001 Rmu_sc0000066.1_g000016
rosa_roxburghii Rroxscaffold_5G00369530
rosa_rugosa Rorug04G0220200
rosa_samantha Rh4AG277200 Rh4BG283000 Rh4DG280100
rosa_wichuraiana Rw4G024080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 96
Acc16I TGCGCA 1 cut(s) 570
AccB1I GGYRCC 1 cut(s) 47
AccBSI CCGCTC 1 cut(s) 454
AciI CCGC 4 cut(s) 25, 207, 454, 722
AcoI YGGCCR 1 cut(s) 1017
AcsI RAATTY 4 cut(s) 73, 116, 821, 910
AcuI CTGAAG 1 cut(s) 1047
AcyI GRCGYC 2 cut(s) 48, 93
AfaI GTAC 3 cut(s) 295, 682, 980
AfiI CCNNNNNNNGG 5 cut(s) 103, 206, 609, 610, 1015
AgsI TTSAA 6 cut(s) 247, 412, 653, 767, 910, 929
AhlI ACTAGT 1 cut(s) 683
AluBI AGCT 6 cut(s) 271, 343, 395, 677, 732, 1067
AluI AGCT 6 cut(s) 271, 343, 395, 677, 732, 1067
AoxI GGCC 1 cut(s) 1017
ApeKI GCWGC 4 cut(s) 323, 592, 666, 693
ApoI RAATTY 4 cut(s) 73, 116, 821, 910
AspLEI GCGC 2 cut(s) 50, 571
AspS9I GGNCC 6 cut(s) 32, 135, 197, 562, 710, 890
AsuC2I CCSGG 2 cut(s) 64, 105
AsuHPI GGTGA 1 cut(s) 569
AvaII GGWCC 6 cut(s) 32, 135, 197, 562, 710, 890
BalI TGGCCA 1 cut(s) 1019
BanI GGYRCC 1 cut(s) 47
BarI GAAGNNNNNNTAC 4 cut(s) 354, 386, 633, 665
BbsI GAAGAC 2 cut(s) 273, 489
BbvCI CCTCAGC 1 cut(s) 396
BbvI GCAGC 4 cut(s) 310, 604, 653, 705
BccI CCATC 2 cut(s) 604, 829
BcgI CGANNNNNNTGC 2 cut(s) 60, 94
BciVI GTATCC 1 cut(s) 972
BcnI CCSGG 2 cut(s) 64, 105
BcuI ACTAGT 1 cut(s) 683
BfaI CTAG 4 cut(s) 581, 678, 684, 726
BfmI CTRYAG 1 cut(s) 371
BfoI RGCGCY 1 cut(s) 51
BfuI GTATCC 1 cut(s) 972
BisI GCNGC 5 cut(s) 324, 593, 667, 694, 723
BlsI GCNGC 5 cut(s) 325, 594, 668, 695, 724
BmcAI AGTACT 2 cut(s) 295, 682
Bme1390I CCNGG 2 cut(s) 64, 105
Bme18I GGWCC 6 cut(s) 32, 135, 197, 562, 710, 890
BmgT120I GGNCC 6 cut(s) 32, 135, 197, 562, 710, 890
BmiI GGNNCC 5 cut(s) 49, 137, 198, 199, 564
BmrFI CCNGG 2 cut(s) 64, 105
BmrI ACTGGG 1 cut(s) 306
BmsI GCATC 3 cut(s) 194, 567, 755
BmuI ACTGGG 1 cut(s) 306
BpiI GAAGAC 2 cut(s) 273, 489
BpmI CTGGAG 1 cut(s) 693
Bpu10I CCTNAGC 1 cut(s) 396
BpuEI CTTGAG 1 cut(s) 312
BpuMI CCSGG 2 cut(s) 64, 105
Bsa29I ATCGAT 1 cut(s) 57
BsaHI GRCGYC 2 cut(s) 48, 93
BsaJI CCNNGG 3 cut(s) 28, 309, 604
BsaXI ACNNNNNCTCC 2 cut(s) 392, 422
Bsc4I CCNNNNNNNGG 5 cut(s) 103, 206, 609, 610, 1015
Bse1I ACTGG 2 cut(s) 301, 676
Bse3DI GCAATG 1 cut(s) 365
BseCI ATCGAT 1 cut(s) 57
BseDI CCNNGG 3 cut(s) 28, 309, 604
BseGI GGATG 2 cut(s) 462, 925
BseLI CCNNNNNNNGG 5 cut(s) 103, 206, 609, 610, 1015
BseMI GCAATG 1 cut(s) 365
BseMII CTCAG 5 cut(s) 299, 387, 495, 542, 747
BseNI ACTGG 2 cut(s) 301, 676
BseRI GAGGAG 2 cut(s) 326, 413
BseXI GCAGC 4 cut(s) 310, 604, 653, 705
BsgI GTGCAG 2 cut(s) 378, 652
BshFI GGCC 1 cut(s) 1019
BshNI GGYRCC 1 cut(s) 47
BshVI ATCGAT 1 cut(s) 57
BsiSI CCGG 5 cut(s) 45, 51, 64, 87, 104
BslFI GGGAC 2 cut(s) 183, 548
BslI CCNNNNNNNGG 5 cut(s) 103, 206, 609, 610, 1015
BsmFI GGGAC 2 cut(s) 183, 548
BsnI GGCC 1 cut(s) 1019
Bsp1407I TGTACA 1 cut(s) 978
Bsp143I GATC 1 cut(s) 231
BspACI CCGC 4 cut(s) 25, 207, 454, 722
BspANI GGCC 1 cut(s) 1019
BspCNI CTCAG 5 cut(s) 298, 388, 496, 543, 746
BspDI ATCGAT 1 cut(s) 57
BspLI GGNNCC 5 cut(s) 49, 137, 198, 199, 564
BspT107I GGYRCC 1 cut(s) 47
BsrBI CCGCTC 1 cut(s) 454
BsrDI GCAATG 1 cut(s) 365
BsrGI TGTACA 1 cut(s) 978
BsrI ACTGG 2 cut(s) 301, 676
BssECI CCNNGG 3 cut(s) 28, 309, 604
BssMI GATC 1 cut(s) 231
BssNI GRCGYC 2 cut(s) 48, 93
BssT1I CCWWGG 2 cut(s) 28, 604
Bst4CI ACNGT 1 cut(s) 476
Bst6I CTCTTC 1 cut(s) 423
BstACI GRCGYC 2 cut(s) 48, 93
BstAPI GCANNNNNTGC 2 cut(s) 577, 786
BstAUI TGTACA 1 cut(s) 978
BstC8I GCNNGC 1 cut(s) 1056
BstDEI CTNAG 6 cut(s) 285, 396, 504, 551, 733, 1068
BstEII GGTNACC 1 cut(s) 640
BstF5I GGATG 2 cut(s) 462, 925
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 2 cut(s) 50, 571
BstKTI GATC 1 cut(s) 234
BstMBI GATC 1 cut(s) 231
BstMWI GCNNNNNNNGC 4 cut(s) 45, 577, 786, 1064
BstNSI RCATGY 1 cut(s) 179
BstPI GGTNACC 1 cut(s) 640
BstSCI CCNGG 2 cut(s) 62, 103
BstSFI CTRYAG 1 cut(s) 371
BstV1I GCAGC 4 cut(s) 310, 604, 653, 705
BstV2I GAAGAC 2 cut(s) 273, 489
BstXI CCANNNNNNTGG 1 cut(s) 1027
Bsu15I ATCGAT 1 cut(s) 57
BsuI GTATCC 1 cut(s) 972
BsuRI GGCC 1 cut(s) 1019
BsuTUI ATCGAT 1 cut(s) 57
BtsCI GGATG 2 cut(s) 462, 925
BtsI GCAGTG 1 cut(s) 703
BtsIMutI CAGTG 2 cut(s) 669, 703
Cac8I GCNNGC 1 cut(s) 1056
CfoI GCGC 2 cut(s) 50, 571
Cfr13I GGNCC 6 cut(s) 32, 135, 197, 562, 710, 890
ClaI ATCGAT 1 cut(s) 57
CseI GACGC 1 cut(s) 267
Csp6I GTAC 3 cut(s) 294, 681, 979
CviAII CATG 4 cut(s) 176, 466, 541, 775
CviQI GTAC 3 cut(s) 294, 681, 979
DdeI CTNAG 6 cut(s) 285, 396, 504, 551, 733, 1068
DinI GGCGCC 1 cut(s) 49
DpnI GATC 1 cut(s) 233
DpnII GATC 1 cut(s) 231
EaeI YGGCCR 1 cut(s) 1017
Eam1104I CTCTTC 1 cut(s) 423
EarI CTCTTC 1 cut(s) 423
Eco130I CCWWGG 2 cut(s) 28, 604
Eco47I GGWCC 6 cut(s) 32, 135, 197, 562, 710, 890
Eco57I CTGAAG 1 cut(s) 1047
Eco91I GGTNACC 1 cut(s) 640
EcoO109I RGGNCCY 3 cut(s) 135, 197, 562
EcoO65I GGTNACC 1 cut(s) 640
EcoRI GAATTC 2 cut(s) 821, 910
EcoT14I CCWWGG 2 cut(s) 28, 604
EcoT22I ATGCAT 1 cut(s) 776
EgeI GGCGCC 1 cut(s) 49
EheI GGCGCC 1 cut(s) 49
ErhI CCWWGG 2 cut(s) 28, 604
FaeI CATG 4 cut(s) 179, 469, 544, 778
FaiI YATR 8 cut(s) 149, 177, 467, 542, 740, 772, 776, 872
FaqI GGGAC 2 cut(s) 183, 548
FatI CATG 4 cut(s) 175, 465, 540, 774
FauI CCCGC 1 cut(s) 32
Fnu4HI GCNGC 5 cut(s) 324, 593, 667, 694, 723
FokI GGATG 2 cut(s) 469, 932
Fsp4HI GCNGC 5 cut(s) 324, 593, 667, 694, 723
FspBI CTAG 4 cut(s) 581, 678, 684, 726
FspI TGCGCA 1 cut(s) 570
GlaI GCGC 2 cut(s) 49, 570
GluI GCNGC 5 cut(s) 324, 593, 667, 694, 723
GsuI CTGGAG 1 cut(s) 693
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 1 cut(s) 1019
HapII CCGG 5 cut(s) 45, 51, 64, 87, 104
HgaI GACGC 1 cut(s) 267
HhaI GCGC 2 cut(s) 50, 571
Hin1I GRCGYC 2 cut(s) 48, 93
Hin1II CATG 4 cut(s) 179, 469, 544, 778
Hin6I GCGC 2 cut(s) 48, 569
HinP1I GCGC 2 cut(s) 48, 569
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HinfI GANTC 6 cut(s) 54, 305, 479, 506, 929, 950
HpaII CCGG 5 cut(s) 45, 51, 64, 87, 104
HphI GGTGA 1 cut(s) 569
Hpy166II GTNNAC 2 cut(s) 97, 538
Hpy188I TCNGA 6 cut(s) 268, 288, 310, 352, 505, 934
Hpy188III TCNNGA 7 cut(s) 12, 235, 653, 798, 826, 917, 1039
Hpy8I GTNNAC 2 cut(s) 97, 538
Hpy99I CGWCG 1 cut(s) 95
HpyCH4III ACNGT 1 cut(s) 476
HpyCH4IV ACGT 1 cut(s) 93
HpyCH4V TGCA 8 cut(s) 71, 359, 592, 669, 774, 789, 864, 882
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 577, 786, 1064
HpyF3I CTNAG 6 cut(s) 285, 396, 504, 551, 733, 1068
HpySE526I ACGT 1 cut(s) 93
Hsp92I GRCGYC 2 cut(s) 48, 93
Hsp92II CATG 4 cut(s) 179, 469, 544, 778
HspAI GCGC 2 cut(s) 48, 569
KasI GGCGCC 1 cut(s) 47
KflI GGGWCCC 1 cut(s) 197
Kzo9I GATC 1 cut(s) 231
LmnI GCTCC 2 cut(s) 268, 674
Lsp1109I GCAGC 4 cut(s) 310, 604, 653, 705
LweI GCATC 3 cut(s) 194, 567, 755
MaeI CTAG 4 cut(s) 581, 678, 684, 726
MaeII ACGT 1 cut(s) 93
MaeIII GTNAC 2 cut(s) 640, 754
MalI GATC 1 cut(s) 233
MbiI CCGCTC 1 cut(s) 454
MboI GATC 1 cut(s) 231
MfeI CAATTG 1 cut(s) 377
MlsI TGGCCA 1 cut(s) 1019
MluCI AATT 6 cut(s) 73, 116, 242, 377, 821, 910
MluNI TGGCCA 1 cut(s) 1019
Mly113I GGCGCC 1 cut(s) 48
MlyI GAGTC 2 cut(s) 515, 944
MmeI TCCRAC 3 cut(s) 246, 282, 795
Mox20I TGGCCA 1 cut(s) 1019
Mph1103I ATGCAT 1 cut(s) 776
MscI TGGCCA 1 cut(s) 1019
MseI TTAA 3 cut(s) 255, 444, 1025
MslI CAYNNNNRTG 1 cut(s) 986
Msp20I TGGCCA 1 cut(s) 1019
MspI CCGG 5 cut(s) 45, 51, 64, 87, 104
MspR9I CCNGG 2 cut(s) 64, 105
MunI CAATTG 1 cut(s) 377
MwoI GCNNNNNNNGC 4 cut(s) 45, 577, 786, 1064
NarI GGCGCC 1 cut(s) 48
NciI CCSGG 2 cut(s) 64, 105
NdeII GATC 1 cut(s) 231
NlaIII CATG 4 cut(s) 179, 469, 544, 778
NlaIV GGNNCC 5 cut(s) 49, 137, 198, 199, 564
NmuCI GTSAC 1 cut(s) 754
NsbI TGCGCA 1 cut(s) 570
NsiI ATGCAT 1 cut(s) 776
NspI RCATGY 1 cut(s) 179
PfeI GAWTC 4 cut(s) 54, 305, 479, 929
PkrI GCNGC 5 cut(s) 325, 594, 668, 695, 724
PleI GAGTC 2 cut(s) 514, 944
PluTI GGCGCC 1 cut(s) 51
PpsI GAGTC 2 cut(s) 514, 944
PpuMI RGGWCCY 3 cut(s) 135, 197, 562
Psp5II RGGWCCY 3 cut(s) 135, 197, 562
PspEI GGTNACC 1 cut(s) 640
PspN4I GGNNCC 5 cut(s) 49, 137, 198, 199, 564
PspPI GGNCC 6 cut(s) 32, 135, 197, 562, 710, 890
PspPPI RGGWCCY 3 cut(s) 135, 197, 562
RsaI GTAC 3 cut(s) 295, 682, 980
RsaNI GTAC 3 cut(s) 294, 681, 979
RseI CAYNNNNRTG 1 cut(s) 986
SaqAI TTAA 3 cut(s) 255, 444, 1025
SatI GCNGC 5 cut(s) 324, 593, 667, 694, 723
Sau3AI GATC 1 cut(s) 231
Sau96I GGNCC 6 cut(s) 32, 135, 197, 562, 710, 890
ScaI AGTACT 2 cut(s) 295, 682
SchI GAGTC 2 cut(s) 515, 944
ScrFI CCNGG 2 cut(s) 64, 105
SfaNI GCATC 3 cut(s) 194, 567, 755
SfcI CTRYAG 1 cut(s) 371
SfoI GGCGCC 1 cut(s) 49
SinI GGWCC 6 cut(s) 32, 135, 197, 562, 710, 890
SmiMI CAYNNNNRTG 1 cut(s) 986
SmlI CTYRAG 1 cut(s) 327
SmoI CTYRAG 1 cut(s) 327
SpeI ACTAGT 1 cut(s) 683
Sse9I AATT 6 cut(s) 73, 116, 242, 377, 821, 910
SsiI CCGC 4 cut(s) 25, 207, 454, 722
SspDI GGCGCC 1 cut(s) 47
SspMI CTAG 4 cut(s) 581, 678, 684, 726
StyD4I CCNGG 2 cut(s) 62, 103
StyI CCWWGG 2 cut(s) 28, 604
TaaI ACNGT 1 cut(s) 476
TaiI ACGT 1 cut(s) 96
TaqI TCGA 5 cut(s) 57, 509, 599, 825, 843
TasI AATT 6 cut(s) 73, 116, 242, 377, 821, 910
TatI WGTACW 3 cut(s) 293, 680, 978
TauI GCSGC 1 cut(s) 725
TfiI GAWTC 4 cut(s) 54, 305, 479, 929
Tru1I TTAA 3 cut(s) 255, 444, 1025
Tru9I TTAA 3 cut(s) 255, 444, 1025
TscAI CASTG 2 cut(s) 676, 703
TseFI GTSAC 1 cut(s) 754
TseI GCWGC 4 cut(s) 323, 592, 666, 693
Tsp45I GTSAC 1 cut(s) 754
TspDTI ATGAA 7 cut(s) 66, 129, 473, 480, 482, 489, 557
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 2 cut(s) 676, 703
VpaK11BI GGWCC 6 cut(s) 32, 135, 197, 562, 710, 890
XapI RAATTY 4 cut(s) 73, 116, 821, 910
XceI RCATGY 1 cut(s) 179
XspI CTAG 4 cut(s) 581, 678, 684, 726
ZraI GACGTC 1 cut(s) 94
ZrmI AGTACT 2 cut(s) 295, 682
Zsp2I ATGCAT 1 cut(s) 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.