Rh4DG280100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0013525
Physical Location & Seq
Forward (+)
50242597 .. 50243187
591 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG280100.1

Sequence Viewer

Length: 591 bp
ATGAAGAACTGTGAATCGTCATCTTCATCGTCATCATCTGAGTCGAGTGAGAGTGAATGTGGGGAGGTGGTTGACATGAACAGCCTGAGAGGTGAGGTCCCTGCGCAACCGATACTAGAGGGTTTGCAGCCATCGACCCAAGGAGGGGTGGCATTATTGACCCAGCCGAGTTCGGTAACCACACTTCAAGAAGATGAATCCTGCACTGGAACTAGTACTAGTTGTGGCAGCAGTGATAGTATTGGTCCTAACAATGCGGCTAGTAGCTCAGTTATGGAAGCATCTGTTGTGACGAAGATTGAAGTATGCATGGGCAAAAAGTGCAAGACATCAGGAGGTGTAGCCTTGTTGGAAGAATTCGAGAGGTTGATGGGTGTCGAAGGAAGTGTTGTTGGGTGCAAGTGTATGGGAAAATGCAAAAATGGTCCAAATGTGAGGGTTTTGAATTCTCTGGATGAAGGAACAGAGGACTCTGTAAGGAATCCCACTAATCCTTTGTACATTGGAGTGGGACTGGAAGATGTGAGCCTCATCGTGGCCAACTTAATGGGGGAAGTCAAGAAGGATTTGGGGCTTGCCACTGCAACTTAG

Protein Analysis

196

Amino Acids

20.14

Weight (kDa)

4.42

Isoelectric Point (pI)

54.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013525)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48300
fragaria_vesca FvH4_4g21650 FvH4_4g21660
malus_domestica MD13G1156700.v1.1 MD16G1134300.v1.1
prunus_persica Prupe.1G103600_v2.0.a1 Prupe.1G103600_v2.0.a1
pyrus_communis pycom13g12050 pycom16g11860
rosa_chinensis RchiOBHm_Chr4g0427881
rosa_multiflora Rmu_co8387153.1_g000001 Rmu_sc0000066.1_g000016
rosa_roxburghii Rroxscaffold_5G00369530
rosa_rugosa Rorug04G0220200
rosa_samantha Rh4AG277200 Rh4BG283000 Rh4DG280100
rosa_wichuraiana Rw4G024080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 105
AciI CCGC 1 cut(s) 257
AcoI YGGCCR 1 cut(s) 537
AcsI RAATTY 2 cut(s) 356, 445
AfaI GTAC 2 cut(s) 217, 500
AfiI CCNNNNNNNGG 3 cut(s) 144, 145, 535
AgsI TTSAA 3 cut(s) 188, 302, 445
AhlI ACTAGT 2 cut(s) 212, 218
AluBI AGCT 1 cut(s) 267
AluI AGCT 1 cut(s) 267
AoxI GGCC 1 cut(s) 537
ApeKI GCWGC 2 cut(s) 127, 228
ApoI RAATTY 2 cut(s) 356, 445
AspLEI GCGC 1 cut(s) 106
AspS9I GGNCC 3 cut(s) 97, 245, 425
AsuHPI GGTGA 1 cut(s) 104
AvaII GGWCC 3 cut(s) 97, 245, 425
BalI TGGCCA 1 cut(s) 539
BarI GAAGNNNNNNTAC 2 cut(s) 168, 200
BbvI GCAGC 2 cut(s) 139, 240
BccI CCATC 2 cut(s) 139, 364
BcuI ACTAGT 2 cut(s) 212, 218
BfaI CTAG 4 cut(s) 116, 213, 219, 261
BisI GCNGC 3 cut(s) 128, 229, 258
BlsI GCNGC 3 cut(s) 129, 230, 259
BmcAI AGTACT 1 cut(s) 217
Bme18I GGWCC 3 cut(s) 97, 245, 425
BmgT120I GGNCC 3 cut(s) 97, 245, 425
BmiI GGNNCC 1 cut(s) 99
BmsI GCATC 1 cut(s) 290
BsaJI CCNNGG 1 cut(s) 139
Bsc4I CCNNNNNNNGG 3 cut(s) 144, 145, 535
Bse1I ACTGG 2 cut(s) 211, 519
BseDI CCNNGG 1 cut(s) 139
BseGI GGATG 1 cut(s) 460
BseLI CCNNNNNNNGG 3 cut(s) 144, 145, 535
BseMII CTCAG 3 cut(s) 30, 77, 282
BseNI ACTGG 2 cut(s) 211, 519
BseXI GCAGC 2 cut(s) 139, 240
BseYI CCCAGC 1 cut(s) 162
BsgI GTGCAG 1 cut(s) 187
BshFI GGCC 1 cut(s) 539
BslFI GGGAC 2 cut(s) 83, 525
BslI CCNNNNNNNGG 3 cut(s) 144, 145, 535
BsmFI GGGAC 2 cut(s) 83, 525
BsnI GGCC 1 cut(s) 539
Bsp1407I TGTACA 1 cut(s) 498
BspACI CCGC 1 cut(s) 257
BspANI GGCC 1 cut(s) 539
BspCNI CTCAG 3 cut(s) 31, 78, 281
BspLI GGNNCC 1 cut(s) 99
BsrGI TGTACA 1 cut(s) 498
BsrI ACTGG 2 cut(s) 211, 519
BssECI CCNNGG 1 cut(s) 139
BssT1I CCWWGG 1 cut(s) 139
Bst4CI ACNGT 1 cut(s) 11
BstAPI GCANNNNNTGC 1 cut(s) 321
BstAUI TGTACA 1 cut(s) 498
BstC8I GCNNGC 1 cut(s) 576
BstDEI CTNAG 4 cut(s) 39, 86, 268, 588
BstEII GGTNACC 1 cut(s) 175
BstF5I GGATG 1 cut(s) 460
BstHHI GCGC 1 cut(s) 106
BstMWI GCNNNNNNNGC 1 cut(s) 321
BstPI GGTNACC 1 cut(s) 175
BstV1I GCAGC 2 cut(s) 139, 240
BstXI CCANNNNNNTGG 1 cut(s) 547
BsuRI GGCC 1 cut(s) 539
BtsCI GGATG 1 cut(s) 460
BtsI GCAGTG 2 cut(s) 238, 579
BtsIMutI CAGTG 3 cut(s) 204, 238, 579
Cac8I GCNNGC 1 cut(s) 576
CfoI GCGC 1 cut(s) 106
Cfr13I GGNCC 3 cut(s) 97, 245, 425
Csp6I GTAC 2 cut(s) 216, 499
CviAII CATG 2 cut(s) 76, 310
CviJI RGCY 9 cut(s) 84, 130, 166, 260, 267, 344, 528, 539, 574
CviKI_1 RGCY 9 cut(s) 84, 130, 166, 260, 267, 344, 528, 539, 574
CviQI GTAC 2 cut(s) 216, 499
DdeI CTNAG 4 cut(s) 39, 86, 268, 588
EaeI YGGCCR 1 cut(s) 537
Eco130I CCWWGG 1 cut(s) 139
Eco47I GGWCC 3 cut(s) 97, 245, 425
Eco91I GGTNACC 1 cut(s) 175
EcoO109I RGGNCCY 1 cut(s) 97
EcoO65I GGTNACC 1 cut(s) 175
EcoRI GAATTC 2 cut(s) 356, 445
EcoT14I CCWWGG 1 cut(s) 139
EcoT22I ATGCAT 1 cut(s) 311
ErhI CCWWGG 1 cut(s) 139
FaeI CATG 2 cut(s) 79, 313
FaiI YATR 5 cut(s) 77, 275, 307, 311, 407
FaqI GGGAC 2 cut(s) 83, 525
FatI CATG 2 cut(s) 75, 309
Fnu4HI GCNGC 3 cut(s) 128, 229, 258
FokI GGATG 1 cut(s) 467
Fsp4HI GCNGC 3 cut(s) 128, 229, 258
FspBI CTAG 4 cut(s) 116, 213, 219, 261
FspI TGCGCA 1 cut(s) 105
GlaI GCGC 1 cut(s) 105
GluI GCNGC 3 cut(s) 128, 229, 258
GsaI CCCAGC 1 cut(s) 166
HaeIII GGCC 1 cut(s) 539
HhaI GCGC 1 cut(s) 106
Hin1II CATG 2 cut(s) 79, 313
Hin6I GCGC 1 cut(s) 104
HinP1I GCGC 1 cut(s) 104
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HinfI GANTC 5 cut(s) 14, 41, 197, 470, 481
HphI GGTGA 1 cut(s) 104
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 1 cut(s) 40
Hpy188III TCNNGA 5 cut(s) 188, 333, 361, 452, 559
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 3 cut(s) 374, 452, 556
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4V TGCA 7 cut(s) 127, 204, 309, 324, 399, 417, 584
HpyF10VI GCNNNNNNNGC 1 cut(s) 321
HpyF3I CTNAG 4 cut(s) 39, 86, 268, 588
Hsp92II CATG 2 cut(s) 79, 313
HspAI GCGC 1 cut(s) 104
LpnPI CCDG 8 cut(s) 98, 114, 176, 192, 214, 318, 437, 500
Lsp1109I GCAGC 2 cut(s) 139, 240
LweI GCATC 1 cut(s) 290
MaeI CTAG 4 cut(s) 116, 213, 219, 261
MaeIII GTNAC 2 cut(s) 175, 289
MboII GAAGA 6 cut(s) 15, 16, 203, 307, 365, 530
MlsI TGGCCA 1 cut(s) 539
MluCI AATT 2 cut(s) 356, 445
MluNI TGGCCA 1 cut(s) 539
MlyI GAGTC 2 cut(s) 50, 464
MmeI TCCRAC 1 cut(s) 330
Mox20I TGGCCA 1 cut(s) 539
Mph1103I ATGCAT 1 cut(s) 311
MscI TGGCCA 1 cut(s) 539
MseI TTAA 1 cut(s) 545
MslI CAYNNNNRTG 1 cut(s) 506
Msp20I TGGCCA 1 cut(s) 539
MwoI GCNNNNNNNGC 1 cut(s) 321
NlaIII CATG 2 cut(s) 79, 313
NlaIV GGNNCC 1 cut(s) 99
NmeAIII GCCGAG 1 cut(s) 192
NmuCI GTSAC 1 cut(s) 289
NsbI TGCGCA 1 cut(s) 105
NsiI ATGCAT 1 cut(s) 311
PfeI GAWTC 3 cut(s) 14, 197, 481
PkrI GCNGC 3 cut(s) 129, 230, 259
PleI GAGTC 2 cut(s) 49, 464
PpsI GAGTC 2 cut(s) 49, 464
PpuMI RGGWCCY 1 cut(s) 97
Psp5II RGGWCCY 1 cut(s) 97
PspEI GGTNACC 1 cut(s) 175
PspFI CCCAGC 1 cut(s) 162
PspN4I GGNNCC 1 cut(s) 99
PspPI GGNCC 3 cut(s) 97, 245, 425
PspPPI RGGWCCY 1 cut(s) 97
RsaI GTAC 2 cut(s) 217, 500
RsaNI GTAC 2 cut(s) 216, 499
RseI CAYNNNNRTG 1 cut(s) 506
SaqAI TTAA 1 cut(s) 545
SatI GCNGC 3 cut(s) 128, 229, 258
Sau96I GGNCC 3 cut(s) 97, 245, 425
ScaI AGTACT 1 cut(s) 217
SchI GAGTC 2 cut(s) 50, 464
SetI ASST 6 cut(s) 69, 94, 99, 269, 340, 368
SfaNI GCATC 1 cut(s) 290
SinI GGWCC 3 cut(s) 97, 245, 425
SmiMI CAYNNNNRTG 1 cut(s) 506
SpeI ACTAGT 2 cut(s) 212, 218
Sse9I AATT 2 cut(s) 356, 445
SsiI CCGC 1 cut(s) 257
SspMI CTAG 4 cut(s) 116, 213, 219, 261
StyI CCWWGG 1 cut(s) 139
TaaI ACNGT 1 cut(s) 11
TaqI TCGA 4 cut(s) 44, 134, 360, 378
TasI AATT 2 cut(s) 356, 445
TatI WGTACW 2 cut(s) 215, 498
TauI GCSGC 1 cut(s) 260
TfiI GAWTC 3 cut(s) 14, 197, 481
Tru1I TTAA 1 cut(s) 545
Tru9I TTAA 1 cut(s) 545
TscAI CASTG 3 cut(s) 211, 238, 586
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 2 cut(s) 127, 228
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 5 cut(s) 15, 17, 92, 210, 471
TspRI CASTG 3 cut(s) 211, 238, 586
VpaK11BI GGWCC 3 cut(s) 97, 245, 425
XapI RAATTY 2 cut(s) 356, 445
XspI CTAG 4 cut(s) 116, 213, 219, 261
ZrmI AGTACT 1 cut(s) 217
Zsp2I ATGCAT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.