FvH4_4g21730
ERF Family

Thioesterase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004722
Physical Location & Seq
Reverse (-)
24722610 .. 24724139
1530 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g21730.t1

Sequence Viewer

Length: 501 bp
ATGGCTCTCCAGTCTCATCCCCAACCGCCACCACCGTCATCGGAGGACAAGACGGAGGCTCTAGATCCTCTGCTTCATGCATTCGGGTTCAAGGTTGAAGAAGTGACACCACATAAGATCTGTGGTCACCTCAGAGTCACCGAAAAGAGCTGCCAGCCATTCAAGGTGTTACATGGAGGTGTCTCAGCTCTGATATCCGAGTCTCTGGCAAGCATGGGAGCTCACCTGGCTTCTGGTTTACAAAGGGTGGCTGGAATTCACCTCAGCATCAATCACATGAAGCGAGCTGAGCTCGGTGATCTTATCTTTGGTGAAGCCTCACCTATCAGCGTTGGCAAAACCATTCAGGTATGGGAGGTCCGACTTTGGAAGATTGATCCTTCAAACTCAGGCTTAAAGTCCTTAGTATCATCATCAAGAGTTACTCTTCTATGCAACATGCCTGTGCCAGAGCATGCAAAAGATGCAAGTCAAGCCCTCAAGAAGTATGCCAAATTATAG

Protein Analysis

167

Amino Acids

17.94

Weight (kDa)

8.81

Isoelectric Point (pI)

49.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT PF03061 55 - 127 2.3e-14 Thioesterase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 26
AclWI GGATC 2 cut(s) 59, 371
AcsI RAATTY 1 cut(s) 255
AgsI TTSAA 4 cut(s) 91, 98, 163, 384
AjnI CCWGG 1 cut(s) 225
AluBI AGCT 5 cut(s) 150, 188, 221, 287, 292
AluI AGCT 5 cut(s) 150, 188, 221, 287, 292
Alw21I GWGCWC 2 cut(s) 223, 294
Alw26I GTCTC 3 cut(s) 18, 187, 207
AlwI GGATC 2 cut(s) 59, 371
ApeKI GCWGC 1 cut(s) 150
ApoI RAATTY 1 cut(s) 255
AspS9I GGNCC 1 cut(s) 358
AsuHPI GGTGA 7 cut(s) 119, 130, 215, 251, 308, 312, 323
AvaII GGWCC 1 cut(s) 358
BanII GRGCYC 2 cut(s) 223, 294
Bbv12I GWGCWC 2 cut(s) 223, 294
BbvCI CCTCAGC 1 cut(s) 263
BbvI GCAGC 1 cut(s) 137
BciT130I CCWGG 1 cut(s) 227
BcoDI GTCTC 3 cut(s) 18, 187, 207
BfaI CTAG 1 cut(s) 62
BglII AGATCT 1 cut(s) 117
BisI GCNGC 1 cut(s) 151
BlpI GCTNAGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 152
Bme1390I CCNGG 1 cut(s) 227
Bme18I GGWCC 1 cut(s) 358
BmgT120I GGNCC 1 cut(s) 358
BmrFI CCNGG 1 cut(s) 227
BmsI GCATC 2 cut(s) 276, 454
BplI GAGNNNNNCTC 2 cut(s) 276, 308
Bpu10I CCTNAGC 1 cut(s) 263
Bpu1102I GCTNAGC 1 cut(s) 288
BpuEI CTTGAG 1 cut(s) 464
Bse1I ACTGG 1 cut(s) 10
BseBI CCWGG 1 cut(s) 227
BseGI GGATG 1 cut(s) 16
BseMII CTCAG 5 cut(s) 145, 198, 277, 279, 402
BseNI ACTGG 1 cut(s) 10
BseXI GCAGC 1 cut(s) 137
BsiHKAI GWGCWC 2 cut(s) 223, 294
BsmAI GTCTC 3 cut(s) 18, 187, 207
BsmI GAATGC 1 cut(s) 80
Bsp1286I GDGCHC 2 cut(s) 223, 294
Bsp143I GATC 4 cut(s) 64, 117, 298, 376
Bsp1720I GCTNAGC 1 cut(s) 288
BspACI CCGC 1 cut(s) 26
BspCNI CTCAG 5 cut(s) 144, 197, 276, 280, 401
BspPI GGATC 2 cut(s) 59, 371
BsrI ACTGG 1 cut(s) 10
BssMI GATC 4 cut(s) 64, 117, 298, 376
Bst2UI CCWGG 1 cut(s) 227
Bst4CI ACNGT 1 cut(s) 36
Bst6I CTCTTC 1 cut(s) 432
BstAPI GCANNNNNTGC 1 cut(s) 464
BstC8I GCNNGC 4 cut(s) 155, 211, 285, 456
BstDEI CTNAG 6 cut(s) 131, 184, 263, 288, 388, 403
BstEII GGTNACC 1 cut(s) 125
BstF5I GGATG 1 cut(s) 16
BstKTI GATC 4 cut(s) 67, 120, 301, 379
BstMAI GTCTC 3 cut(s) 18, 187, 207
BstMBI GATC 4 cut(s) 64, 117, 298, 376
BstMWI GCNNNNNNNGC 4 cut(s) 227, 289, 464, 473
BstNI CCWGG 1 cut(s) 227
BstNSI RCATGY 2 cut(s) 442, 458
BstPI GGTNACC 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 225
BstV1I GCAGC 1 cut(s) 137
BstX2I RGATCY 2 cut(s) 64, 117
BstYI RGATCY 2 cut(s) 64, 117
BtsCI GGATG 1 cut(s) 16
Cac8I GCNNGC 4 cut(s) 155, 211, 285, 456
Cfr13I GGNCC 1 cut(s) 358
CviAII CATG 6 cut(s) 77, 173, 214, 277, 439, 455
DdeI CTNAG 6 cut(s) 131, 184, 263, 288, 388, 403
DpnI GATC 4 cut(s) 66, 119, 300, 378
DpnII GATC 4 cut(s) 64, 117, 298, 376
Eam1104I CTCTTC 1 cut(s) 432
EarI CTCTTC 1 cut(s) 432
Ecl136II GAGCTC 2 cut(s) 221, 292
Eco24I GRGCYC 2 cut(s) 223, 294
Eco32I GATATC 1 cut(s) 195
Eco47I GGWCC 1 cut(s) 358
Eco53kI GAGCTC 2 cut(s) 221, 292
Eco91I GGTNACC 1 cut(s) 125
EcoICRI GAGCTC 2 cut(s) 221, 292
EcoO65I GGTNACC 1 cut(s) 125
EcoRI GAATTC 1 cut(s) 255
EcoRII CCWGG 1 cut(s) 225
EcoRV GATATC 1 cut(s) 195
EcoT22I ATGCAT 1 cut(s) 82
EcoT38I GRGCYC 2 cut(s) 223, 294
FaeI CATG 6 cut(s) 80, 176, 217, 280, 442, 458
FatI CATG 6 cut(s) 76, 172, 213, 276, 438, 454
Fnu4HI GCNGC 1 cut(s) 151
FokI GGATG 1 cut(s) 3
FriOI GRGCYC 2 cut(s) 223, 294
Fsp4HI GCNGC 1 cut(s) 151
FspBI CTAG 1 cut(s) 62
GluI GCNGC 1 cut(s) 151
Hin1II CATG 6 cut(s) 80, 176, 217, 280, 442, 458
HinfI GANTC 2 cut(s) 135, 200
HphI GGTGA 7 cut(s) 119, 130, 215, 251, 308, 312, 323
Hpy166II GTNNAC 1 cut(s) 239
Hpy188I TCNGA 5 cut(s) 43, 134, 192, 199, 362
Hpy188III TCNNGA 3 cut(s) 62, 417, 481
Hpy8I GTNNAC 1 cut(s) 239
HpyAV CCTTC 1 cut(s) 390
HpyCH4III ACNGT 1 cut(s) 36
HpyCH4V TGCA 4 cut(s) 80, 435, 458, 467
HpyF10VI GCNNNNNNNGC 4 cut(s) 227, 289, 464, 473
HpyF3I CTNAG 6 cut(s) 131, 184, 263, 288, 388, 403
Hsp92II CATG 6 cut(s) 80, 176, 217, 280, 442, 458
Kzo9I GATC 4 cut(s) 64, 117, 298, 376
LmnI GCTCC 1 cut(s) 218
Lsp1109I GCAGC 1 cut(s) 137
LweI GCATC 2 cut(s) 276, 454
MaeI CTAG 1 cut(s) 62
MaeIII GTNAC 5 cut(s) 103, 125, 136, 168, 421
MalI GATC 4 cut(s) 66, 119, 300, 378
MboI GATC 4 cut(s) 64, 117, 298, 376
MboII GAAGA 3 cut(s) 110, 382, 419
MflI RGATCY 2 cut(s) 64, 117
MhlI GDGCHC 2 cut(s) 223, 294
MluCI AATT 2 cut(s) 255, 494
MlyI GAGTC 2 cut(s) 144, 209
MmeI TCCRAC 1 cut(s) 385
MnlI CCTC 9 cut(s) 37, 49, 78, 140, 170, 272, 328, 349, 488
Mph1103I ATGCAT 1 cut(s) 82
MseI TTAA 1 cut(s) 395
MslI CAYNNNNRTG 2 cut(s) 177, 443
MspR9I CCNGG 1 cut(s) 227
Mva1269I GAATGC 1 cut(s) 80
MvaI CCWGG 1 cut(s) 227
MwoI GCNNNNNNNGC 4 cut(s) 227, 289, 464, 473
NdeII GATC 4 cut(s) 64, 117, 298, 376
NlaIII CATG 6 cut(s) 80, 176, 217, 280, 442, 458
NmuCI GTSAC 3 cut(s) 103, 125, 136
NsiI ATGCAT 1 cut(s) 82
NspI RCATGY 2 cut(s) 442, 458
PaeI GCATGC 1 cut(s) 458
PctI GAATGC 1 cut(s) 80
PkrI GCNGC 1 cut(s) 152
PleI GAGTC 2 cut(s) 143, 208
PpsI GAGTC 2 cut(s) 143, 208
Psp124BI GAGCTC 2 cut(s) 223, 294
Psp6I CCWGG 1 cut(s) 225
PspEI GGTNACC 1 cut(s) 125
PspGI CCWGG 1 cut(s) 225
PspPI GGNCC 1 cut(s) 358
PsuI RGATCY 2 cut(s) 64, 117
RseI CAYNNNNRTG 2 cut(s) 177, 443
SacI GAGCTC 2 cut(s) 223, 294
SaqAI TTAA 1 cut(s) 395
SatI GCNGC 1 cut(s) 151
Sau3AI GATC 4 cut(s) 64, 117, 298, 376
Sau96I GGNCC 1 cut(s) 358
SchI GAGTC 2 cut(s) 144, 209
ScrFI CCNGG 1 cut(s) 227
SduI GDGCHC 2 cut(s) 223, 294
SfaNI GCATC 2 cut(s) 276, 454
SinI GGWCC 1 cut(s) 358
SmiMI CAYNNNNRTG 2 cut(s) 177, 443
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
SphI GCATGC 1 cut(s) 458
Sse9I AATT 2 cut(s) 255, 494
SsiI CCGC 1 cut(s) 26
SspMI CTAG 1 cut(s) 62
SstI GAGCTC 2 cut(s) 223, 294
StyD4I CCNGG 1 cut(s) 225
TaaI ACNGT 1 cut(s) 36
TasI AATT 2 cut(s) 255, 494
Tru1I TTAA 1 cut(s) 395
Tru9I TTAA 1 cut(s) 395
TseFI GTSAC 3 cut(s) 103, 125, 136
TseI GCWGC 1 cut(s) 150
Tsp45I GTSAC 3 cut(s) 103, 125, 136
TspDTI ATGAA 2 cut(s) 65, 293
TspGWI ACGGA 1 cut(s) 68
VpaK11BI GGWCC 1 cut(s) 358
XapI RAATTY 1 cut(s) 255
XbaI TCTAGA 1 cut(s) 61
XceI RCATGY 2 cut(s) 442, 458
XspI CTAG 1 cut(s) 62
Zsp2I ATGCAT 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.