Rroxscaffold_5G00369610
ERF Family

Thioesterase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004722
Physical Location & Seq
Reverse (-)
51408416 .. 51409683
1268 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00369610.1

Sequence Viewer

Length: 534 bp
ATGGCTCCTCAGTCCCGGTCCCAGTCCCAGCCACCCCCACCGTCATCGGAGGACAAGACGGAGGCTCTGGATGCTCTGCTTCATGCATTCAGGTTCGAGGTTGAAGAAGTGACACCCCGAAAGGTCTCTGGTCACCTCAGAGTCACCGAAAAGAGCTGTCAGCCATTCAAGGTGTTACATGGAGGTGTGTCAGCTCTGATATCCGAGTCTCTGGCTAGCATGGGAGCTCACCTGGCTTCTGGTTTACAAAGGGTGGCTGGAATTCACCTCAGCATCAACCACTTGAACCGAGCTGAGCTCGGTGATCTTATCTACACTGAAGCCTCACCCATCAGCGTTGGCAAAACCATTCAGGTTCATGTTCCACTGTACCCTCTTGTAGTTTGGGAGGTGCGATTTTCAAAGATGGATCCTTCAAACTCAGACATCAAATCCCTAGTATCATCATCAAGAGTTACTCTTCTGTGCAACATGCATGTGCCAGAGCATGCAAAAGATGCAGGTCAAGCCCTCAAGAAGTATGCCAAATTATAG

Protein Analysis

177

Amino Acids

19.28

Weight (kDa)

8.57

Isoelectric Point (pI)

54.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT PF03061 57 - 119 3e-09 Thioesterase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 491
AclWI GGATC 2 cut(s) 404, 417
AcsI RAATTY 1 cut(s) 261
AcuI CTGAAG 1 cut(s) 339
AfaI GTAC 1 cut(s) 371
AgsI TTSAA 5 cut(s) 104, 169, 286, 402, 417
AjnI CCWGG 1 cut(s) 231
AluBI AGCT 5 cut(s) 156, 194, 227, 293, 298
AluI AGCT 5 cut(s) 156, 194, 227, 293, 298
Alw21I GWGCWC 2 cut(s) 229, 300
Alw26I GTCTC 2 cut(s) 130, 213
AlwI GGATC 2 cut(s) 404, 417
ApoI RAATTY 1 cut(s) 261
AspS9I GGNCC 1 cut(s) 18
AsuC2I CCSGG 1 cut(s) 16
AsuHPI GGTGA 6 cut(s) 125, 136, 221, 257, 314, 318
AsuNHI GCTAGC 1 cut(s) 215
AvaII GGWCC 1 cut(s) 18
BamHI GGATCC 1 cut(s) 409
BanII GRGCYC 2 cut(s) 229, 300
Bbv12I GWGCWC 2 cut(s) 229, 300
BbvCI CCTCAGC 1 cut(s) 269
BccI CCATC 2 cut(s) 338, 400
BciT130I CCWGG 1 cut(s) 233
BcnI CCSGG 1 cut(s) 16
BcoDI GTCTC 2 cut(s) 130, 213
BfaI CTAG 2 cut(s) 216, 437
BfuAI ACCTGC 1 cut(s) 491
BlpI GCTNAGC 1 cut(s) 294
Bme1390I CCNGG 2 cut(s) 16, 233
Bme18I GGWCC 1 cut(s) 18
BmgT120I GGNCC 1 cut(s) 18
BmiI GGNNCC 3 cut(s) 6, 20, 411
BmrFI CCNGG 2 cut(s) 16, 233
BmrI ACTGGG 1 cut(s) 16
BmsI GCATC 3 cut(s) 61, 282, 487
BmtI GCTAGC 1 cut(s) 219
BmuI ACTGGG 1 cut(s) 16
BplI GAGNNNNNCTC 2 cut(s) 282, 314
Bpu10I CCTNAGC 1 cut(s) 269
Bpu1102I GCTNAGC 1 cut(s) 294
BpuEI CTTGAG 1 cut(s) 497
BpuMI CCSGG 1 cut(s) 16
BsaI GGTCTC 1 cut(s) 130
Bse1I ACTGG 1 cut(s) 22
BseBI CCWGG 1 cut(s) 233
BseGI GGATG 1 cut(s) 76
BseMII CTCAG 5 cut(s) 23, 151, 283, 285, 435
BseNI ACTGG 1 cut(s) 22
BseYI CCCAGC 1 cut(s) 27
BsiHKAI GWGCWC 2 cut(s) 229, 300
BsiSI CCGG 1 cut(s) 16
BslFI GGGAC 2 cut(s) 4, 10
BsmAI GTCTC 2 cut(s) 130, 213
BsmFI GGGAC 2 cut(s) 4, 10
BsmI GAATGC 1 cut(s) 86
Bso31I GGTCTC 1 cut(s) 130
Bsp1286I GDGCHC 2 cut(s) 229, 300
Bsp143I GATC 2 cut(s) 304, 409
Bsp1720I GCTNAGC 1 cut(s) 294
BspCNI CTCAG 5 cut(s) 22, 150, 282, 286, 434
BspLI GGNNCC 3 cut(s) 6, 20, 411
BspMI ACCTGC 1 cut(s) 491
BspOI GCTAGC 1 cut(s) 219
BspPI GGATC 2 cut(s) 404, 417
BspTNI GGTCTC 1 cut(s) 130
BsrI ACTGG 1 cut(s) 22
BssMI GATC 2 cut(s) 304, 409
Bst2UI CCWGG 1 cut(s) 233
Bst4CI ACNGT 2 cut(s) 42, 369
Bst6I CTCTTC 1 cut(s) 465
BstAPI GCANNNNNTGC 1 cut(s) 497
BstC8I GCNNGC 2 cut(s) 217, 489
BstDEI CTNAG 5 cut(s) 9, 137, 269, 294, 421
BstEII GGTNACC 1 cut(s) 131
BstF5I GGATG 1 cut(s) 76
BstKTI GATC 2 cut(s) 307, 412
BstMAI GTCTC 2 cut(s) 130, 213
BstMBI GATC 2 cut(s) 304, 409
BstMWI GCNNNNNNNGC 4 cut(s) 71, 233, 497, 506
BstNI CCWGG 1 cut(s) 233
BstNSI RCATGY 3 cut(s) 475, 479, 491
BstPI GGTNACC 1 cut(s) 131
BstSCI CCNGG 2 cut(s) 14, 231
BstX2I RGATCY 1 cut(s) 409
BstYI RGATCY 1 cut(s) 409
BtsCI GGATG 1 cut(s) 76
BtsIMutI CAGTG 2 cut(s) 315, 365
BveI ACCTGC 1 cut(s) 491
Cac8I GCNNGC 2 cut(s) 217, 489
Cfr13I GGNCC 1 cut(s) 18
Csp6I GTAC 1 cut(s) 370
CviAII CATG 7 cut(s) 83, 179, 220, 359, 472, 476, 488
CviQI GTAC 1 cut(s) 370
DdeI CTNAG 5 cut(s) 9, 137, 269, 294, 421
DpnI GATC 2 cut(s) 306, 411
DpnII GATC 2 cut(s) 304, 409
Eam1104I CTCTTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 465
Ecl136II GAGCTC 2 cut(s) 227, 298
Eco24I GRGCYC 2 cut(s) 229, 300
Eco31I GGTCTC 1 cut(s) 130
Eco32I GATATC 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 18
Eco53kI GAGCTC 2 cut(s) 227, 298
Eco57I CTGAAG 1 cut(s) 339
Eco91I GGTNACC 1 cut(s) 131
EcoICRI GAGCTC 2 cut(s) 227, 298
EcoO65I GGTNACC 1 cut(s) 131
EcoRI GAATTC 1 cut(s) 261
EcoRII CCWGG 1 cut(s) 231
EcoRV GATATC 1 cut(s) 201
EcoT22I ATGCAT 2 cut(s) 88, 477
EcoT38I GRGCYC 2 cut(s) 229, 300
FaeI CATG 7 cut(s) 86, 182, 223, 362, 475, 479, 491
FaiI YATR 9 cut(s) 84, 180, 221, 360, 473, 477, 489, 522, 532
FaqI GGGAC 2 cut(s) 4, 10
FatI CATG 7 cut(s) 82, 178, 219, 358, 471, 475, 487
FokI GGATG 1 cut(s) 83
FriOI GRGCYC 2 cut(s) 229, 300
FspBI CTAG 2 cut(s) 216, 437
GsaI CCCAGC 1 cut(s) 31
HapII CCGG 1 cut(s) 16
Hin1II CATG 7 cut(s) 86, 182, 223, 362, 475, 479, 491
HinfI GANTC 2 cut(s) 141, 206
HpaII CCGG 1 cut(s) 16
HphI GGTGA 6 cut(s) 125, 136, 221, 257, 314, 318
Hpy166II GTNNAC 1 cut(s) 245
Hpy188I TCNGA 5 cut(s) 49, 140, 198, 205, 424
Hpy188III TCNNGA 3 cut(s) 68, 450, 514
Hpy8I GTNNAC 1 cut(s) 245
HpyAV CCTTC 1 cut(s) 423
HpyCH4III ACNGT 2 cut(s) 42, 369
HpyCH4V TGCA 5 cut(s) 86, 468, 475, 491, 500
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 233, 497, 506
HpyF3I CTNAG 5 cut(s) 9, 137, 269, 294, 421
Hsp92II CATG 7 cut(s) 86, 182, 223, 362, 475, 479, 491
Kzo9I GATC 2 cut(s) 304, 409
LmnI GCTCC 2 cut(s) 10, 224
LweI GCATC 3 cut(s) 61, 282, 487
MaeI CTAG 2 cut(s) 216, 437
MaeIII GTNAC 5 cut(s) 109, 131, 142, 174, 454
MalI GATC 2 cut(s) 306, 411
MboI GATC 2 cut(s) 304, 409
MboII GAAGA 2 cut(s) 116, 452
MflI RGATCY 1 cut(s) 409
MhlI GDGCHC 2 cut(s) 229, 300
MluCI AATT 2 cut(s) 261, 527
MlyI GAGTC 2 cut(s) 150, 215
Mph1103I ATGCAT 2 cut(s) 88, 477
MslI CAYNNNNRTG 2 cut(s) 183, 476
MspI CCGG 1 cut(s) 16
MspR9I CCNGG 2 cut(s) 16, 233
Mva1269I GAATGC 1 cut(s) 86
MvaI CCWGG 1 cut(s) 233
MwoI GCNNNNNNNGC 4 cut(s) 71, 233, 497, 506
NciI CCSGG 1 cut(s) 16
NdeII GATC 2 cut(s) 304, 409
NheI GCTAGC 1 cut(s) 215
NlaIII CATG 7 cut(s) 86, 182, 223, 362, 475, 479, 491
NlaIV GGNNCC 3 cut(s) 6, 20, 411
NmuCI GTSAC 3 cut(s) 109, 131, 142
NsiI ATGCAT 2 cut(s) 88, 477
NspI RCATGY 3 cut(s) 475, 479, 491
PaeI GCATGC 1 cut(s) 491
PctI GAATGC 1 cut(s) 86
PleI GAGTC 2 cut(s) 149, 214
PpsI GAGTC 2 cut(s) 149, 214
Psp124BI GAGCTC 2 cut(s) 229, 300
Psp6I CCWGG 1 cut(s) 231
PspEI GGTNACC 1 cut(s) 131
PspFI CCCAGC 1 cut(s) 27
PspGI CCWGG 1 cut(s) 231
PspN4I GGNNCC 3 cut(s) 6, 20, 411
PspPI GGNCC 1 cut(s) 18
PsuI RGATCY 1 cut(s) 409
RsaI GTAC 1 cut(s) 371
RsaNI GTAC 1 cut(s) 370
RseI CAYNNNNRTG 2 cut(s) 183, 476
SacI GAGCTC 2 cut(s) 229, 300
Sau3AI GATC 2 cut(s) 304, 409
Sau96I GGNCC 1 cut(s) 18
SchI GAGTC 2 cut(s) 150, 215
ScrFI CCNGG 2 cut(s) 16, 233
SduI GDGCHC 2 cut(s) 229, 300
SfaNI GCATC 3 cut(s) 61, 282, 487
SinI GGWCC 1 cut(s) 18
SmiMI CAYNNNNRTG 2 cut(s) 183, 476
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
SphI GCATGC 1 cut(s) 491
Sse9I AATT 2 cut(s) 261, 527
SspMI CTAG 2 cut(s) 216, 437
SstI GAGCTC 2 cut(s) 229, 300
StyD4I CCNGG 2 cut(s) 14, 231
TaaI ACNGT 2 cut(s) 42, 369
TaqI TCGA 1 cut(s) 96
TasI AATT 2 cut(s) 261, 527
TscAI CASTG 2 cut(s) 322, 372
TseFI GTSAC 3 cut(s) 109, 131, 142
Tsp45I GTSAC 3 cut(s) 109, 131, 142
TspDTI ATGAA 2 cut(s) 71, 347
TspGWI ACGGA 1 cut(s) 74
TspRI CASTG 2 cut(s) 322, 372
VpaK11BI GGWCC 1 cut(s) 18
XapI RAATTY 1 cut(s) 261
XceI RCATGY 3 cut(s) 475, 479, 491
XspI CTAG 2 cut(s) 216, 437
Zsp2I ATGCAT 2 cut(s) 88, 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.