RchiOBHm_Chr4g0427941
ERF Family

Thioesterase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004722
Physical Location & Seq
Reverse (-)
53062249 .. 53063281
1033 bp
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UTR
Exon/CDS
Intron
PRQ39685

Sequence Viewer

Length: 288 bp
ATGGGAGCTCACCTGGCTTCTAGTTTACAAAGGGTGGCTGGAATCCACCTCAGTATCAACCACTTGAACCGAGCTGAGCTCGGTGATCTTATTTACACTGAAGCCTCACCCATCAGCGTTGGCAAAACCATTCAGGTTTGGGAGGTGCGACTTTCAAAGATAGATCCTTCAAACTCAGACATCAAATCCCTAGTATCATCATCAAGAGTTACTCTTCTGTGCAACATGCATGTGCCAGAGCATGCAAAAGATGCAGGTCAAGCCCTCAAGAAGTATGCCAAATTATAG

Protein Analysis

95

Amino Acids

10.33

Weight (kDa)

9.25

Isoelectric Point (pI)

32.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT PF03061 7 - 55 5.2e-08 Thioesterase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 245
AclWI GGATC 1 cut(s) 158
AcuI CTGAAG 1 cut(s) 120
AgsI TTSAA 3 cut(s) 67, 156, 171
AjnI CCWGG 1 cut(s) 12
AluBI AGCT 3 cut(s) 8, 74, 79
AluI AGCT 3 cut(s) 8, 74, 79
Alw21I GWGCWC 2 cut(s) 10, 81
AlwI GGATC 1 cut(s) 158
AsuHPI GGTGA 2 cut(s) 95, 99
BaeI ACNNNNGTAYC 2 cut(s) 37, 70
BanII GRGCYC 2 cut(s) 10, 81
Bbv12I GWGCWC 2 cut(s) 10, 81
BccI CCATC 1 cut(s) 119
BciT130I CCWGG 1 cut(s) 14
BfaI CTAG 2 cut(s) 21, 191
BfuAI ACCTGC 1 cut(s) 245
BlpI GCTNAGC 1 cut(s) 75
Bme1390I CCNGG 1 cut(s) 14
BmrFI CCNGG 1 cut(s) 14
BmsI GCATC 1 cut(s) 241
BplI GAGNNNNNCTC 2 cut(s) 63, 95
Bpu1102I GCTNAGC 1 cut(s) 75
BpuEI CTTGAG 1 cut(s) 251
BseBI CCWGG 1 cut(s) 14
BseMII CTCAG 3 cut(s) 64, 66, 189
BsiHKAI GWGCWC 2 cut(s) 10, 81
Bsp1286I GDGCHC 2 cut(s) 10, 81
Bsp143I GATC 2 cut(s) 85, 163
Bsp1720I GCTNAGC 1 cut(s) 75
BspCNI CTCAG 3 cut(s) 63, 67, 188
BspMI ACCTGC 1 cut(s) 245
BspPI GGATC 1 cut(s) 158
BssMI GATC 2 cut(s) 85, 163
Bst2UI CCWGG 1 cut(s) 14
Bst6I CTCTTC 1 cut(s) 219
BstAPI GCANNNNNTGC 1 cut(s) 251
BstC8I GCNNGC 1 cut(s) 243
BstDEI CTNAG 3 cut(s) 50, 75, 175
BstKTI GATC 2 cut(s) 88, 166
BstMBI GATC 2 cut(s) 85, 163
BstMWI GCNNNNNNNGC 3 cut(s) 14, 251, 260
BstNI CCWGG 1 cut(s) 14
BstNSI RCATGY 3 cut(s) 229, 233, 245
BstSCI CCNGG 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 163
BstYI RGATCY 1 cut(s) 163
BtsIMutI CAGTG 1 cut(s) 96
BveI ACCTGC 1 cut(s) 245
Cac8I GCNNGC 1 cut(s) 243
CviAII CATG 3 cut(s) 226, 230, 242
CviJI RGCY 7 cut(s) 8, 17, 38, 74, 79, 104, 263
CviKI_1 RGCY 7 cut(s) 8, 17, 38, 74, 79, 104, 263
DdeI CTNAG 3 cut(s) 50, 75, 175
DpnI GATC 2 cut(s) 87, 165
DpnII GATC 2 cut(s) 85, 163
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
Ecl136II GAGCTC 2 cut(s) 8, 79
Eco24I GRGCYC 2 cut(s) 10, 81
Eco53kI GAGCTC 2 cut(s) 8, 79
Eco57I CTGAAG 1 cut(s) 120
EcoICRI GAGCTC 2 cut(s) 8, 79
EcoRII CCWGG 1 cut(s) 12
EcoT22I ATGCAT 1 cut(s) 231
EcoT38I GRGCYC 2 cut(s) 10, 81
FaeI CATG 3 cut(s) 229, 233, 245
FaiI YATR 5 cut(s) 227, 231, 243, 276, 286
FatI CATG 3 cut(s) 225, 229, 241
FriOI GRGCYC 2 cut(s) 10, 81
FspBI CTAG 2 cut(s) 21, 191
Hin1II CATG 3 cut(s) 229, 233, 245
HinfI GANTC 1 cut(s) 42
HphI GGTGA 2 cut(s) 95, 99
Hpy166II GTNNAC 1 cut(s) 26
Hpy188I TCNGA 1 cut(s) 178
Hpy188III TCNNGA 2 cut(s) 204, 268
Hpy8I GTNNAC 1 cut(s) 26
HpyAV CCTTC 1 cut(s) 177
HpyCH4V TGCA 4 cut(s) 222, 229, 245, 254
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 251, 260
HpyF3I CTNAG 3 cut(s) 50, 75, 175
Hsp92II CATG 3 cut(s) 229, 233, 245
Kzo9I GATC 2 cut(s) 85, 163
LmnI GCTCC 1 cut(s) 5
LpnPI CCDG 5 cut(s) 24, 26, 119, 240, 249
LweI GCATC 1 cut(s) 241
MaeI CTAG 2 cut(s) 21, 191
MaeIII GTNAC 1 cut(s) 208
MalI GATC 2 cut(s) 87, 165
MboI GATC 2 cut(s) 85, 163
MboII GAAGA 1 cut(s) 206
MflI RGATCY 1 cut(s) 163
MhlI GDGCHC 2 cut(s) 10, 81
MluCI AATT 1 cut(s) 281
MnlI CCTC 4 cut(s) 59, 115, 136, 275
Mph1103I ATGCAT 1 cut(s) 231
MslI CAYNNNNRTG 1 cut(s) 230
MspR9I CCNGG 1 cut(s) 14
MvaI CCWGG 1 cut(s) 14
MwoI GCNNNNNNNGC 3 cut(s) 14, 251, 260
NdeII GATC 2 cut(s) 85, 163
NlaIII CATG 3 cut(s) 229, 233, 245
NsiI ATGCAT 1 cut(s) 231
NspI RCATGY 3 cut(s) 229, 233, 245
PaeI GCATGC 1 cut(s) 245
PfeI GAWTC 1 cut(s) 42
Psp124BI GAGCTC 2 cut(s) 10, 81
Psp6I CCWGG 1 cut(s) 12
PspGI CCWGG 1 cut(s) 12
PsuI RGATCY 1 cut(s) 163
RseI CAYNNNNRTG 1 cut(s) 230
SacI GAGCTC 2 cut(s) 10, 81
Sau3AI GATC 2 cut(s) 85, 163
ScrFI CCNGG 1 cut(s) 14
SduI GDGCHC 2 cut(s) 10, 81
SetI ASST 8 cut(s) 10, 15, 51, 76, 81, 138, 147, 259
SfaNI GCATC 1 cut(s) 241
SmiMI CAYNNNNRTG 1 cut(s) 230
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
SphI GCATGC 1 cut(s) 245
Sse9I AATT 1 cut(s) 281
SspMI CTAG 2 cut(s) 21, 191
SstI GAGCTC 2 cut(s) 10, 81
StyD4I CCNGG 1 cut(s) 12
TasI AATT 1 cut(s) 281
TfiI GAWTC 1 cut(s) 42
TscAI CASTG 1 cut(s) 103
TspRI CASTG 1 cut(s) 103
XceI RCATGY 3 cut(s) 229, 233, 245
XspI CTAG 2 cut(s) 21, 191
Zsp2I ATGCAT 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.