Rorug04G0220700
ERF Family

Thioesterase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004722
Physical Location & Seq
Reverse (-)
38019646 .. 38022023
2378 bp
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UTR
Exon/CDS
Intron
Rorug04G0220700.1

Sequence Viewer

Length: 459 bp
ATGTCTCTCGTGGCGAACGAGGAGTTCCAGCACATTCTGCGTGTTCTGAACACCAACGTCGATGGAAAGCAGAAGATCATGTTCGCTCTCACCTCCATCAAGGGTATCGGTCGCCGTTTAGCCAACATCGTCTGCAAGAAGGCCGACGTCGACATGAACAAGAGGGCTGGTGAGCTGTCTGCTCAGGAGATTGAGAACCTGATGTACATTGTGAGTAATCCCCGCCAGTTCAAGATCCCTGACTGGTTTCTCAACAGAAAGAAGGATTACAAGGATGGTAAATATTCCCAGGTGGTTGCCAATGCCTTGGATATGAAGCTCAGGGATGATCTCGAGAGGCTCAAGAAGATCAGGAATCATCGTGGTCTTCGACACTACTGGGGCCTTCGCGTGCGTGGACAGCACACCAAGACCACTGGCCGCAGGGGGAAGACTGTTGGTGTGTCAAAGAAACGTTGA

Protein Analysis

152

Amino Acids

17.68

Weight (kDa)

10.48

Isoelectric Point (pI)

36.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S13 PF00416 14 - 142 1.3e-56 Ribosomal protein S13/S18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AccI GTMKAC 1 cut(s) 150
AccII CGCG 1 cut(s) 390
AciI CCGC 2 cut(s) 223, 421
AclI AACGTT 1 cut(s) 454
AclWI GGATC 1 cut(s) 229
AcoI YGGCCR 1 cut(s) 418
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 1 cut(s) 206
AgsI TTSAA 1 cut(s) 232
AjnI CCWGG 1 cut(s) 288
AluBI AGCT 2 cut(s) 175, 319
AluI AGCT 2 cut(s) 175, 319
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 1 cut(s) 229
Ama87I CYCGRG 1 cut(s) 332
AoxI GGCC 3 cut(s) 141, 382, 418
AspS9I GGNCC 1 cut(s) 382
AsuHPI GGTGA 2 cut(s) 82, 182
AvaI CYCGRG 1 cut(s) 332
BauI CACGAG 1 cut(s) 8
BbsI GAAGAC 2 cut(s) 359, 437
BccI CCATC 3 cut(s) 56, 104, 269
BceAI ACGGC 1 cut(s) 99
BciT130I CCWGG 1 cut(s) 290
BcoDI GTCTC 1 cut(s) 9
BisI GCNGC 1 cut(s) 421
BlsI GCNGC 1 cut(s) 422
Bme1390I CCNGG 1 cut(s) 290
BmeT110I CYCGRG 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 382
BmiI GGNNCC 1 cut(s) 383
BmrFI CCNGG 1 cut(s) 290
BmrI ACTGGG 1 cut(s) 388
BmuI ACTGGG 1 cut(s) 388
BpiI GAAGAC 2 cut(s) 359, 437
Bpu10I CCTNAGC 2 cut(s) 183, 320
BpuEI CTTGAG 1 cut(s) 326
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 2 cut(s) 288, 306
Bse1I ACTGG 4 cut(s) 226, 248, 383, 421
BseBI CCWGG 1 cut(s) 290
BseDI CCNNGG 2 cut(s) 288, 306
BseGI GGATG 2 cut(s) 280, 331
BseMII CTCAG 2 cut(s) 197, 334
BseNI ACTGG 4 cut(s) 226, 248, 383, 421
BseRI GAGGAG 1 cut(s) 35
Bsh1236I CGCG 1 cut(s) 390
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 3 cut(s) 143, 384, 420
BsiEI CGRYCG 1 cut(s) 112
BsiHKCI CYCGRG 1 cut(s) 332
BsmAI GTCTC 1 cut(s) 9
BsnI GGCC 3 cut(s) 143, 384, 420
BsoBI CYCGRG 1 cut(s) 332
Bsp1407I TGTACA 1 cut(s) 204
Bsp143I GATC 4 cut(s) 75, 234, 328, 348
BspACI CCGC 2 cut(s) 223, 421
BspANI GGCC 3 cut(s) 143, 384, 420
BspCNI CTCAG 2 cut(s) 196, 333
BspFNI CGCG 1 cut(s) 390
BspLI GGNNCC 1 cut(s) 383
BspPI GGATC 1 cut(s) 229
BsrGI TGTACA 1 cut(s) 204
BsrI ACTGG 4 cut(s) 226, 248, 383, 421
BssECI CCNNGG 2 cut(s) 288, 306
BssMI GATC 4 cut(s) 75, 234, 328, 348
BssNI GRCGYC 1 cut(s) 147
BssSI CACGAG 1 cut(s) 8
BssT1I CCWWGG 1 cut(s) 306
Bst2BI CACGAG 1 cut(s) 8
Bst2UI CCWGG 1 cut(s) 290
Bst4CI ACNGT 1 cut(s) 436
BstACI GRCGYC 1 cut(s) 147
BstAPI GCANNNNNTGC 1 cut(s) 37
BstAUI TGTACA 1 cut(s) 204
BstC8I GCNNGC 1 cut(s) 392
BstDEI CTNAG 2 cut(s) 183, 320
BstF5I GGATG 2 cut(s) 280, 331
BstFNI CGCG 1 cut(s) 390
BstKTI GATC 4 cut(s) 78, 237, 331, 351
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 4 cut(s) 75, 234, 328, 348
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 2 cut(s) 37, 400
BstNI CCWGG 1 cut(s) 290
BstSCI CCNGG 1 cut(s) 288
BstUI CGCG 1 cut(s) 390
BstV2I GAAGAC 2 cut(s) 359, 437
BstX2I RGATCY 1 cut(s) 234
BstXI CCANNNNNNTGG 1 cut(s) 307
BstYI RGATCY 1 cut(s) 234
BsuRI GGCC 3 cut(s) 143, 384, 420
BtsCI GGATG 2 cut(s) 280, 331
BtsIMutI CAGTG 1 cut(s) 414
Cac8I GCNNGC 1 cut(s) 392
Cfr13I GGNCC 1 cut(s) 382
Csp6I GTAC 1 cut(s) 205
CviAII CATG 2 cut(s) 79, 154
CviJI RGCY 8 cut(s) 122, 143, 167, 175, 319, 340, 384, 420
CviKI_1 RGCY 8 cut(s) 122, 143, 167, 175, 319, 340, 384, 420
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 2 cut(s) 183, 320
DpnI GATC 4 cut(s) 77, 236, 330, 350
DpnII GATC 4 cut(s) 75, 234, 328, 348
EaeI YGGCCR 1 cut(s) 418
Eco130I CCWWGG 1 cut(s) 306
Eco88I CYCGRG 1 cut(s) 332
EcoO109I RGGNCCY 1 cut(s) 382
EcoRII CCWGG 1 cut(s) 288
EcoT14I CCWWGG 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 2 cut(s) 82, 157
FaiI YATR 3 cut(s) 80, 155, 314
FatI CATG 2 cut(s) 78, 153
FauI CCCGC 1 cut(s) 230
FblI GTMKAC 1 cut(s) 150
Fnu4HI GCNGC 1 cut(s) 421
FokI GGATG 2 cut(s) 287, 338
Fsp4HI GCNGC 1 cut(s) 421
GluI GCNGC 1 cut(s) 421
HaeIII GGCC 3 cut(s) 143, 384, 420
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 2 cut(s) 82, 157
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HinfI GANTC 1 cut(s) 355
HphI GGTGA 2 cut(s) 82, 182
Hpy166II GTNNAC 2 cut(s) 151, 398
Hpy188I TCNGA 1 cut(s) 48
Hpy188III TCNNGA 6 cut(s) 185, 232, 332, 334, 343, 352
Hpy8I GTNNAC 2 cut(s) 151, 398
Hpy99I CGWCG 3 cut(s) 62, 149, 152
HpyAV CCTTC 3 cut(s) 133, 256, 395
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4IV ACGT 3 cut(s) 57, 147, 454
HpyCH4V TGCA 1 cut(s) 135
HpyF10VI GCNNNNNNNGC 2 cut(s) 37, 400
HpyF3I CTNAG 2 cut(s) 183, 320
HpySE526I ACGT 3 cut(s) 57, 147, 454
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 2 cut(s) 82, 157
Kzo9I GATC 4 cut(s) 75, 234, 328, 348
MaeII ACGT 3 cut(s) 57, 147, 454
MalI GATC 4 cut(s) 77, 236, 330, 350
MboI GATC 4 cut(s) 75, 234, 328, 348
MboII GAAGA 4 cut(s) 85, 358, 359, 442
MflI RGATCY 1 cut(s) 234
MnlI CCTC 4 cut(s) 13, 103, 156, 330
MspR9I CCNGG 1 cut(s) 290
MvaI CCWGG 1 cut(s) 290
MvnI CGCG 1 cut(s) 390
MwoI GCNNNNNNNGC 2 cut(s) 37, 400
NdeII GATC 4 cut(s) 75, 234, 328, 348
NlaIII CATG 2 cut(s) 82, 157
NlaIV GGNNCC 1 cut(s) 383
PaeR7I CTCGAG 1 cut(s) 332
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 367
PfeI GAWTC 1 cut(s) 355
PkrI GCNGC 1 cut(s) 422
Psp1406I AACGTT 1 cut(s) 454
Psp6I CCWGG 1 cut(s) 288
PspGI CCWGG 1 cut(s) 288
PspN4I GGNNCC 1 cut(s) 383
PspPI GGNCC 1 cut(s) 382
PsrI GAACNNNNNNTAC 2 cut(s) 188, 220
PsuI RGATCY 1 cut(s) 234
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
SalI GTCGAC 1 cut(s) 149
SatI GCNGC 1 cut(s) 421
Sau3AI GATC 4 cut(s) 75, 234, 328, 348
Sau96I GGNCC 1 cut(s) 382
ScrFI CCNGG 1 cut(s) 290
SetI ASST 8 cut(s) 60, 95, 150, 177, 201, 294, 321, 457
Sfr274I CTCGAG 1 cut(s) 332
SlaI CTCGAG 1 cut(s) 332
SmlI CTYRAG 2 cut(s) 332, 341
SmoI CTYRAG 2 cut(s) 332, 341
SsiI CCGC 2 cut(s) 223, 421
SspI AATATT 1 cut(s) 284
StyD4I CCNGG 1 cut(s) 288
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 1 cut(s) 436
TaiI ACGT 3 cut(s) 60, 150, 457
TaqI TCGA 4 cut(s) 60, 150, 333, 370
TaqII GACCGA 1 cut(s) 98
TatI WGTACW 1 cut(s) 204
TauI GCSGC 1 cut(s) 423
TfiI GAWTC 1 cut(s) 355
TscAI CASTG 1 cut(s) 421
TspDTI ATGAA 2 cut(s) 170, 329
TspRI CASTG 1 cut(s) 421
XhoI CTCGAG 1 cut(s) 332
XmiI GTMKAC 1 cut(s) 150
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.