Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0005249

Physical Location & Seq

Genomic Coordinates Forward (+)

27461069 .. 27474154

Gene Structure 13086 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g25800.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4203 bp

ATGAGCAGCTCGGTGAGCCATAATTTGCTGCAGAGCAAAATCAATCCTTCAGGCATTCCTGCCAACACATTGTTACGGCCCAAGTGTGTACATCAAGTCCCAGCGCAGCCGCGGAAGTCTTCTTTATCCAAGAAGTTCTGTGGCAACAGTTTGCAGAAGCCCAAGCTCGCCATGGGGTCTCGCAGACCTGTTACATTTGTTCCTTGTGCTGTACTGACCACGGATCAGCGTTCTGATCAGCTCGCCAGTAAATACAACCTTGATGGCAACATTGAATTGCAGGTTTATGTAGATGCTTCCTCTCCAGGGTCTACTAAAGTAGATATCCATGTCTCAAATAGCGGTGATTCTTTGGTTCTTCATTGGGGTGGGATACAAGATAGAAAAGAAAACTGGGTGCTTCCTTCTCGTCACCCAGATGGAACCACAGTATATAAGAAGAAAGCTCTTAGAACTCCTTTTGCAAAATCTGGTCCCAATTCCTCACTTAAAATCGAGATTGATGATCCTGCAATACAAGCTATAGAGTTTCTCATAGTTGATGAAAGCCAGAACAGATGGTTTAAGAACAACGGCGGCAACTTTCATGTTAAGTTACCTTTGAAAGAGATGTCGATATCAAATGTTTCAGTTCCTGAAGATTTAGTGCAAATTCAAGCGTATCTGAGGTGGGAGCGAAAGGGTAAACAGATGTATACACCAGAGCAAGAGAAGGTGGAGTACGAAGCAGCCCGAACTGAGCTATTGGAGGAAGTAGCCAGGGGTGCTTCCATACAAGAGCTCCAAGCAAGGCTGACTAAGAAAAGCGATTCTGGTAACAGTCATGAACAATCTCAAGCTGGTAACAAAGAGGTCTCCAAGGCCGCAAAGAAACATGAGAGTAAACAAGTCTACAGAACTGGAAGAATTAACCGGAAACAACGAGACTTGATGAAGATTCTCAACAAACATACTACTAAACCTGTAGATGAAGCTAAACTCGCAAATGAAGAATCAGCAAAACCAAAAGCCTTGAAAGCAGTTGAGCTTTTTGCCAAGGAAAAAGAAGAACAAGATGGTGCTTCCACTCTGAACAAACAAATCTACAAGCTCGGTGATAAGGACCTTCTGGTACTTGTGACAAAGGTCGCTGACAAGATAAGGGTTCATCTGGCAACAGACATCAAAGAGCCAGTTAACCTTCATTGGGCTCTATCTGTAAACAGGGCCGGAGAGTGGTTGGAACCACCTCCAAATGTACTACCCCAGGGTTCAGTTTCTCTAAATGGGGCTGTTGAAACACAATTTGTATCCAGTTCTGTTGATTCTACTTATGAGATCCAATCTTTAGAAATAGAAACGCAAGAGAAAAGTTTCAAAGGAATGCCATTTGTCCTTTGTTCTTCTGGAAACTGGATTAAGAACCAAGGCTCAGATTTCTATGCCGACTTTGGTGTTGCAGTCAAGAAAGTTCAGAAGGATGCTGGTGATGGAAAAGGTACTGCAAAGGCTTTATTGGATAATATAGCAGATCTGGAGAGTGAGGCGCAAAAATCCTTTATGCATAGGTTTAACATTGCAGCAGACTTGATGAGTCAAGCAAAAGATGCTGGTGCATTAGGTCTTGCAGGTATTTTGGTGTGGATGAGGTTTATGGCTACAAGGCAGCTCATTTGGAATAAAAATTACAATGTCAAACCACGTGAGATTAGTAAAGCACAGGACAGACTCACAGACTTGCTCCAGAGTATTTACATAAGTTATCCACAGCATCGAGAGCTTCTACGGATGATATTGTCTACTGTTGGACGTGGAGGAGAAGGGGATGTGGGACAACGAATTCGAGATGAGATCCTTGTCATCCAGAGAAACTGCGATTGTAAAGGTGGAATGATGGAGGAATGGCATCAAAAGTTACATAATAACACTAGCCCAGATGATGTTGTTATATGTCAGGCACTAATTGATTACATCAAAAGTGACTTTGATATTGGTGTTTATTGGAAAACTTTGAACGATAATGGAATCACAAAAGAGCGCCTTCTAAGCTATGACCGGGCAATCCATAATGAACCAAGATTCAGGGCAGATCAGAAAGACCGTCTTCTGCATGATCTGGGGAACTACTTGAGAACTTTAAAGGCAGTTCACTCAGGTGCAGATCTTGAGTCTGCCATCACAAATTGCTTGGGTTACAGTGCTAAGGGTCAAGGCTTTATGGTTGGAGTACAAATAAATCCTGTATCTGGCTTGCCCTCTGAAGTTCCAGGGCTGCTGCAATTTGTAATGGAACATGTTGAGGACAGGAATGTGGAAGTCCTTGTTGAGGGTTTGCTTGAGGCTCGTCAGGAGATCTGGCCATTGCTCTCTAAGCCTAATGATCGCCTGAAGGATCTCTTATTTTTGGACATTGCCCTTGATTCTACTGTGAGAACTGCCATTGAGAGAGGATATGAAGAACTGAATAATGCTGGGCCAGAGAAAATTATGTACTTCATTTCAATGGTTCTAGAGAACCTGGCATTATCATCAGACGATAATGAGGACCTTCTCTACTGTTTGAAGGGATGGAATCAGGCTTTAAACATGTTAAAGAATAATGCCGATCACTGGGCATTATTTGCAAAATCAATTCTAGATAGAACCCGCCTGGCGCTTGCAAACAAGGCAGAGTTATATCACAGTATTTTACAGCCATCAGCAGAGTACCTTGGATCAAAGCTTGGAGTGGACAAATTGGCTTTGAGCATATTTACTGAAGAAGTTATCCGTGCTGGATCAGCTGCCTCTTTATCCACACTTGTTAATCGACTTGATCCAGTTCTTCGGAAGACTGCTAACTTGGGAAGTTGGCAGGTTATCAGCCCAGTTGAAGTGGTTGGATATGTTGTAGTTGTGGACGAGTTGCTCACTGTCCAGAATAAAGTATACGATAAGCCAACAATTTTGGTGGCAAGAAGTGTGAGAGGTGAGGAAGAAATTCCAGATGGCACAGTTGCTGTGCTAACACCTGATATGCCAGACGTCTTATCTCATGTATCTGTCCGAGCAAGAAATAGCAAGGTTTGTTTCGCGACATGTTTTGACTCCAATATTCTAGCTGACCTCCAAGCTTGTGAAGGGAAGTTATTGCGCGTAAAACCAACTTCTGCTGACGTAGTTTATAGTGAGGTTAATGAGAGTGAGCTAGGAGATGCAAGTTCAACTAATTTAAATGAAGACACCCCAGCACTGACATTAGTGAAGAAACAGTTTACGGGTAGATATGCCATATCATCTGATGAGTTCACAAGTGAAATGGTGGGAGCTAAATCACGTAATATTTCTTATATAAAAGGAAAACTGCCCTCTTGGGTTGGAATTCCTACATCAGTTGCCCTACCTTTTGGAGTATTTGAAAAAGTTCTTTCTGAAGACTCAAATAAGGCTGTTGCTGACAAGATAGAAACTCTAAAGAAAAAGTTAAAAGAAGGTGATTTTGGTTCACTAGGGGAGATTCGAGAGACAGTTTTACAGTTGACAGCACCACCCCCATTGGTGCAAGAGCTGAAGTCTAAGATGCAAAGTTCAGGAATGCCTTGGCCTGGTGATGAGGGTGAACAACGATGGGAACAAGCATGGCTGTCTATAAAGAAGGTCTGGGCTTCGAAGTGGAATGAGAGAGCATACTTCAGCACAAGAAAAGTGAAATTAGATCATGATTATCTTTGCATGGCTGTCCTTGTTCAAGAAATAATAAATGCTGACTATGCATTTGTTATTCACACAACTAACCCATCTTCAGGAGACTCGTCGGAAATATATGCCGAGGTTGTCAAGGGACTTGGAGAAACCTTAGTTGGAGCTTATCCTGGACGTGCTTTAAGTTTTATTAGCAAGAAAAATGATCTGGACTCTCCTCAGCTGTTAGGTTACCCTAGCAAACCGATTGGCCTCTTTATAAGACGGTCCATAATCTTCCGATCTGATTCCAATGGTGAAGATCTTGAAGGTTATGCTGGTGCAGGCCTTTATGACAGTGTGCCTATGGATAAGGAAGAGGAAGTCGTGCTTGACTACTCATCTGACCCACTCGTTACTGATGGGAACTTCCAAAAGAAAATTCTATCAAGCATTGCTCATGCAGGAAATGCAATCGAGGAGTTATATGGTTTGCCACAAGATATTGAAGGTGTGATCAGAGATGGCAAACTCTATGTTGTCCAGACAAGACCCCAGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009628 GO:0009631 GO:0009941 GO:0009987 GO:0016052 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016781 GO:0031967 GO:0031975 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050521 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPDK_N

Protein Analysis

1401

Amino Acids

155.59

Weight (kDa)

6.13

Isoelectric Point (pI)

41.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ig_N_CWD1	PF23166	83 - 199	4.4e-40	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
DUF7067	PF23229	213 - 268	4.1e-28	Alpha-glucan water dikinase, DUF7067
Ig_N_CWD1	PF23166	358 - 476	7.8e-27	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
GWD1_pHisD	PF22973	943 - 1055	8.1e-54	Alpha-glucan water dikinase, phosphohistidine-like domain
PPDK_N	PF01326	1200 - 1399	1e-19	Pyruvate phosphate dikinase, AMP/ATP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10760 AT1G10760 AT1G10760
fragaria_vesca	FvH4_4g25800 FvH4_4g25800 FvH4_4g25800 FvH4_4g25800
malus_domestica	MD13G1096800.v1.1 MD16G1097800.v1.1
prunus_persica	Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1
pyrus_communis	pycom13g08450 pycom16g08330
rosa_chinensis	RchiOBHm_Chr4g0433251
rosa_laevigata	RLG00000006771
rosa_multiflora	Rmu_co8055492.1_g000001 Rmu_sc0010643.1_g000001
rosa_roxburghii	Rroxscaffold_5G00374810
rosa_rugosa	Rorug04G0267200 Rorug04G0267300 Rorug04G0267400 Rorug04G0267400
rosa_samantha	Rh4AG321900 Rh4BG329300 Rh4CG344600
rosa_wichuraiana	Rw4G027860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3920
AatII	GACGTC	1 cut(s)	3006
Acc36I	ACCTGC	3 cut(s)	271, 1598, 2824
AccI	GTMKAC	5 cut(s)	311, 695, 891, 1778, 2907
AccII	CGCG	3 cut(s)	112, 3053, 3114
AciI	CCGC	6 cut(s)	110, 112, 342, 576, 864, 2626
AclWI	GGATC	8 cut(s)	231, 500, 1312, 1825, 2379, 2701, 2764, 2789
AcoI	YGGCCR	1 cut(s)	2336
AcsI	RAATTY	5 cut(s)	651, 1818, 2958, 3339, 4080
AcuI	CTGAAG	9 cut(s)	33, 657, 2259, 2387, 2757, 3411, 3548, 3634, 3744
AcvI	CACGTG	1 cut(s)	1682
AcyI	GRCGYC	1 cut(s)	3003
AfaI	GTAC	9 cut(s)	90, 213, 722, 1113, 1239, 1480, 2208, 2471, 2687
AfiI	CCNNNNNNNGG	8 cut(s)	306, 1186, 1215, 2225, 2305, 2589, 3563, 3761
AflIII	ACRYGT	3 cut(s)	2272, 2565, 3056
AjiI	CACGTC	2 cut(s)	1790, 3836
AjnI	CCWGG	8 cut(s)	304, 758, 1245, 2245, 2496, 2628, 3562, 3829
AjuI	GAANNNNNNNTTGG	6 cut(s)	3441, 3473, 3801, 3833, 4065, 4097
AleI	CACNNNNGTG	1 cut(s)	2133
Alw21I	GWGCWC	1 cut(s)	783
Alw26I	GTCTC	6 cut(s)	183, 337, 859, 918, 3476, 3759
AlwI	GGATC	8 cut(s)	231, 500, 1312, 1825, 2379, 2701, 2764, 2789
AlwNI	CAGNNNCTG	2 cut(s)	635, 2978
AoxI	GGCC	8 cut(s)	77, 861, 1206, 2336, 2453, 3560, 3910, 3985
ApeKI	GCWGC	9 cut(s)	6, 28, 106, 728, 1559, 1645, 2251, 2254, 2762
ApoI	RAATTY	5 cut(s)	651, 1818, 2958, 3339, 4080
ArsI	GACNNNNNNTTYG	4 cut(s)	1803, 1835, 3716, 3748
Asp700I	GAANNNNTTC	3 cut(s)	1352, 2958, 3381
AspLEI	GCGC	5 cut(s)	106, 1528, 2019, 2635, 3114
AspS9I	GGNCC	7 cut(s)	78, 473, 1102, 1206, 2453, 2524, 3927
AsuC2I	CCSGG	1 cut(s)	2036
AsuII	TTCGAA	1 cut(s)	3626
AvaII	GGWCC	4 cut(s)	473, 1102, 2524, 3927
BalI	TGGCCA	1 cut(s)	2338
BanII	GRGCYC	2 cut(s)	783, 1192
BarI	GAAGNNNNNNTAC	2 cut(s)	704, 736
BbrPI	CACGTG	1 cut(s)	1682
BbsI	GAAGAC	5 cut(s)	111, 2075, 2816, 3204, 3399
Bbv12I	GWGCWC	1 cut(s)	783
BbvCI	CCTCAGC	1 cut(s)	3879
BbvI	GCAGC	9 cut(s)	15, 18, 118, 740, 1571, 1657, 2238, 2241, 2749
BceAI	ACGGC	2 cut(s)	92, 589
BciT130I	CCWGG	8 cut(s)	306, 760, 1247, 2247, 2498, 2630, 3564, 3831
BciVI	GTATCC	2 cut(s)	366, 1300
BclI	TGATCA	2 cut(s)	235, 4155
BcnI	CCSGG	1 cut(s)	2036
BcoDI	GTCTC	6 cut(s)	183, 337, 859, 918, 3476, 3759
BfaI	CTAG	7 cut(s)	1908, 2489, 2615, 3077, 3167, 3467, 3897
BfmI	CTRYAG	4 cut(s)	29, 522, 892, 963
BfoI	RGCGCY	2 cut(s)	2020, 2636
BfuAI	ACCTGC	3 cut(s)	271, 1598, 2824
BfuI	GTATCC	2 cut(s)	366, 1300
BglII	AGATCT	4 cut(s)	1510, 2140, 2331, 3961
Bme1390I	CCNGG	9 cut(s)	306, 760, 1247, 2036, 2247, 2498, 2630, 3564, 3831
Bme18I	GGWCC	4 cut(s)	473, 1102, 2524, 3927
BmgBI	CACGTC	2 cut(s)	1790, 3836
BmgT120I	GGNCC	7 cut(s)	78, 473, 1102, 1206, 2453, 2524, 3927
BmiI	GGNNCC	3 cut(s)	424, 475, 1224
BmrFI	CCNGG	9 cut(s)	306, 760, 1247, 2036, 2247, 2498, 2630, 3564, 3831
BmrI	ACTGGG	3 cut(s)	403, 2599, 2840
BmsI	GCATC	7 cut(s)	283, 1450, 1576, 1759, 1894, 3163, 3528
BmuI	ACTGGG	3 cut(s)	403, 2599, 2840
BoxI	GACNNNNGTC	1 cut(s)	1124
BpiI	GAAGAC	5 cut(s)	111, 2075, 2816, 3204, 3399
BpmI	CTGGAG	3 cut(s)	288, 1535, 1706
Bpu10I	CCTNAGC	2 cut(s)	2181, 3879
Bpu14I	TTCGAA	1 cut(s)	3626
BpuEI	CTTGAG	4 cut(s)	819, 2128, 2165, 2336
BpuMI	CCSGG	1 cut(s)	2036
BsaAI	YACGTR	2 cut(s)	1682, 3296
BsaHI	GRCGYC	1 cut(s)	3003
BsaI	GGTCTC	2 cut(s)	183, 859
BsaWI	WCCGGW	1 cut(s)	912
BsaXI	ACNNNNNCTCC	2 cut(s)	765, 795
Bsc4I	CCNNNNNNNGG	8 cut(s)	306, 1186, 1215, 2225, 2305, 2589, 3563, 3761
Bse1I	ACTGG	9 cut(s)	246, 398, 904, 1172, 1293, 1397, 2594, 2798, 2846
Bse3DI	GCAATG	4 cut(s)	1554, 2339, 2388, 4092
BseBI	CCWGG	8 cut(s)	306, 760, 1247, 2247, 2498, 2630, 3564, 3831
BseGI	GGATG	6 cut(s)	1465, 1629, 1773, 1810, 1839, 2552
BseLI	CCNNNNNNNGG	8 cut(s)	306, 1186, 1215, 2225, 2305, 2589, 3563, 3761
BseMI	GCAATG	4 cut(s)	1554, 2339, 2388, 4092
BseMII	CTCAG	5 cut(s)	656, 729, 1425, 2145, 3893
BseNI	ACTGG	9 cut(s)	246, 398, 904, 1172, 1293, 1397, 2594, 2798, 2846
BseRI	GAGGAG	3 cut(s)	1809, 3867, 4133
BseXI	GCAGC	9 cut(s)	15, 18, 118, 740, 1571, 1657, 2238, 2241, 2749
BseYI	CCCAGC	3 cut(s)	100, 2450, 3205
BsgI	GTGCAG	2 cut(s)	2157, 4002
Bsh1236I	CGCG	3 cut(s)	112, 3053, 3114
BshFI	GGCC	8 cut(s)	79, 863, 1208, 2338, 2455, 3562, 3912, 3987
BsiHKAI	GWGCWC	1 cut(s)	783
BsiSI	CCGG	3 cut(s)	913, 1209, 2035
BslFI	GGGAC	4 cut(s)	83, 459, 1824, 3813
BslI	CCNNNNNNNGG	8 cut(s)	306, 1186, 1215, 2225, 2305, 2589, 3563, 3761
BsmAI	GTCTC	6 cut(s)	183, 337, 859, 918, 3476, 3759
BsmFI	GGGAC	4 cut(s)	83, 459, 1824, 3813
BsmI	GAATGC	3 cut(s)	54, 1368, 3558
BsnI	GGCC	8 cut(s)	79, 863, 1208, 2338, 2455, 3562, 3912, 3987
Bso31I	GGTCTC	2 cut(s)	183, 859
Bsp119I	TTCGAA	1 cut(s)	3626
Bsp1286I	GDGCHC	2 cut(s)	783, 1192
Bsp1407I	TGTACA	1 cut(s)	88
Bsp19I	CCATGG	1 cut(s)	171
Bsp68I	TCGCGA	1 cut(s)	3053
BspACI	CCGC	6 cut(s)	110, 112, 342, 576, 864, 2626
BspANI	GGCC	8 cut(s)	79, 863, 1208, 2338, 2455, 3562, 3912, 3987
BspCNI	CTCAG	5 cut(s)	657, 730, 1424, 2144, 3892
BspFNI	CGCG	3 cut(s)	112, 3053, 3114
BspHI	TCATGA	2 cut(s)	823, 3676
BspLI	GGNNCC	3 cut(s)	424, 475, 1224
BspMAI	CTGCAG	1 cut(s)	33
BspMI	ACCTGC	3 cut(s)	271, 1598, 2824
BspPI	GGATC	8 cut(s)	231, 500, 1312, 1825, 2379, 2701, 2764, 2789
BspT104I	TTCGAA	1 cut(s)	3626
BspTNI	GGTCTC	2 cut(s)	183, 859
BsrDI	GCAATG	4 cut(s)	1554, 2339, 2388, 4092
BsrGI	TGTACA	1 cut(s)	88
BsrI	ACTGG	9 cut(s)	246, 398, 904, 1172, 1293, 1397, 2594, 2798, 2846
BssNAI	GTATAC	2 cut(s)	696, 2908
BssNI	GRCGYC	1 cut(s)	3003
BssT1I	CCWWGG	6 cut(s)	171, 858, 1035, 1405, 2689, 3557
Bst1107I	GTATAC	2 cut(s)	696, 2908
Bst2UI	CCWGG	8 cut(s)	306, 760, 1247, 2247, 2498, 2630, 3564, 3831
Bst6I	CTCTTC	1 cut(s)	4011
BstACI	GRCGYC	1 cut(s)	3003
BstAPI	GCANNNNNTGC	3 cut(s)	1586, 2600, 4109
BstAUI	TGTACA	1 cut(s)	88
BstBAI	YACGTR	2 cut(s)	1682, 3296
BstBI	TTCGAA	1 cut(s)	3626
BstC8I	GCNNGC	5 cut(s)	168, 243, 2231, 2637, 3985
BstDSI	CCRYGG	3 cut(s)	110, 171, 219
BstEII	GGTNACC	1 cut(s)	3890
BstENI	CCTNNNNNAGG	1 cut(s)	2303
BstF5I	GGATG	6 cut(s)	1465, 1629, 1773, 1810, 1839, 2552
BstFNI	CGCG	3 cut(s)	112, 3053, 3114
BstH2I	RGCGCY	2 cut(s)	2020, 2636
BstHHI	GCGC	5 cut(s)	106, 1528, 2019, 2635, 3114
BstMAI	GTCTC	6 cut(s)	183, 337, 859, 918, 3476, 3759
BstNI	CCWGG	8 cut(s)	306, 760, 1247, 2247, 2498, 2630, 3564, 3831
BstNSI	RCATGY	3 cut(s)	2276, 2569, 3060
BstPAI	GACNNNNGTC	1 cut(s)	1124
BstPI	GGTNACC	1 cut(s)	3890
BstSCI	CCNGG	9 cut(s)	304, 758, 1245, 2034, 2245, 2496, 2628, 3562, 3829
BstSFI	CTRYAG	4 cut(s)	29, 522, 892, 963
BstUI	CGCG	3 cut(s)	112, 3053, 3114
BstV1I	GCAGC	9 cut(s)	15, 18, 118, 740, 1571, 1657, 2238, 2241, 2749
BstV2I	GAAGAC	5 cut(s)	111, 2075, 2816, 3204, 3399
BstX2I	RGATCY	7 cut(s)	1317, 1510, 1830, 2140, 2331, 2371, 3961
BstYI	RGATCY	7 cut(s)	1317, 1510, 1830, 2140, 2331, 2371, 3961
BstZ17I	GTATAC	2 cut(s)	696, 2908
BsuI	GTATCC	2 cut(s)	366, 1300
BsuRI	GGCC	8 cut(s)	79, 863, 1208, 2338, 2455, 3562, 3912, 3987
BtgI	CCRYGG	3 cut(s)	110, 171, 219
BtrI	CACGTC	2 cut(s)	1790, 3836
BtsCI	GGATG	6 cut(s)	1465, 1629, 1773, 1810, 1839, 2552
BtsIMutI	CAGTG	5 cut(s)	2182, 2587, 2889, 3209, 4003
BtuMI	TCGCGA	1 cut(s)	3053
BveI	ACCTGC	3 cut(s)	271, 1598, 2824
Cac8I	GCNNGC	5 cut(s)	168, 243, 2231, 2637, 3985
CaiI	CAGNNNCTG	2 cut(s)	635, 2978
CciI	TCATGA	2 cut(s)	823, 3676
CfoI	GCGC	5 cut(s)	106, 1528, 2019, 2635, 3114
Cfr13I	GGNCC	7 cut(s)	78, 473, 1102, 1206, 2453, 2524, 3927
Cfr42I	CCGCGG	1 cut(s)	113
Csp6I	GTAC	9 cut(s)	89, 212, 721, 1112, 1238, 1479, 2207, 2470, 2686
CspCI	CAANNNNNGTGG	4 cut(s)	2910, 2945, 3495, 3530
CviQI	GTAC	9 cut(s)	89, 212, 721, 1112, 1238, 1479, 2207, 2470, 2686
DraI	TTTAAA	3 cut(s)	2118, 2562, 3192
EaeI	YGGCCR	1 cut(s)	2336
Eam1104I	CTCTTC	1 cut(s)	4011
EarI	CTCTTC	1 cut(s)	4011
Ecl136II	GAGCTC	1 cut(s)	781
Eco130I	CCWWGG	6 cut(s)	171, 858, 1035, 1405, 2689, 3557
Eco147I	AGGCCT	1 cut(s)	3987
Eco24I	GRGCYC	2 cut(s)	783, 1192
Eco31I	GGTCTC	2 cut(s)	183, 859
Eco32I	GATATC	2 cut(s)	325, 618
Eco47I	GGWCC	4 cut(s)	473, 1102, 2524, 3927
Eco53kI	GAGCTC	1 cut(s)	781
Eco57I	CTGAAG	9 cut(s)	33, 657, 2259, 2387, 2757, 3411, 3548, 3634, 3744
Eco72I	CACGTG	1 cut(s)	1682
Eco91I	GGTNACC	1 cut(s)	3890
EcoICRI	GAGCTC	1 cut(s)	781
EcoNI	CCTNNNNNAGG	1 cut(s)	2303
EcoO109I	RGGNCCY	2 cut(s)	1102, 2524
EcoO65I	GGTNACC	1 cut(s)	3890
EcoRI	GAATTC	2 cut(s)	1818, 3339
EcoRII	CCWGG	8 cut(s)	304, 758, 1245, 2245, 2496, 2628, 3562, 3829
EcoRV	GATATC	2 cut(s)	325, 618
EcoT14I	CCWWGG	6 cut(s)	171, 858, 1035, 1405, 2689, 3557
EcoT22I	ATGCAT	2 cut(s)	1545, 3733
EcoT38I	GRGCYC	2 cut(s)	783, 1192
ErhI	CCWWGG	6 cut(s)	171, 858, 1035, 1405, 2689, 3557
FalI	AAGNNNNNCTT	8 cut(s)	2004, 2036, 2067, 2099, 2360, 2392, 4014, 4046
FaqI	GGGAC	4 cut(s)	83, 459, 1824, 3813
FauI	CCCGC	1 cut(s)	2633
FbaI	TGATCA	2 cut(s)	235, 4155
FblI	GTMKAC	5 cut(s)	311, 695, 891, 1778, 2907
FokI	GGATG	6 cut(s)	1472, 1636, 1780, 1817, 1826, 2559
FriOI	GRGCYC	2 cut(s)	783, 1192
FspBI	CTAG	7 cut(s)	1908, 2489, 2615, 3077, 3167, 3467, 3897
GlaI	GCGC	5 cut(s)	105, 1527, 2018, 2634, 3113
GsaI	CCCAGC	3 cut(s)	104, 2454, 3209
GsuI	CTGGAG	3 cut(s)	288, 1535, 1706
HaeII	RGCGCY	2 cut(s)	2020, 2636
HaeIII	GGCC	8 cut(s)	79, 863, 1208, 2338, 2455, 3562, 3912, 3987
HapII	CCGG	3 cut(s)	913, 1209, 2035
HhaI	GCGC	5 cut(s)	106, 1528, 2019, 2635, 3114
Hin1I	GRCGYC	1 cut(s)	3003
Hin6I	GCGC	5 cut(s)	104, 1526, 2017, 2633, 3112
HinP1I	GCGC	5 cut(s)	104, 1526, 2017, 2633, 3112
HincII	GTYRAC	2 cut(s)	1177, 3498
HindII	GTYRAC	2 cut(s)	1177, 3498
HindIII	AAGCTT	2 cut(s)	2699, 3090
HpaI	GTTAAC	1 cut(s)	1177
HpaII	CCGG	3 cut(s)	913, 1209, 2035
Hpy99I	CGWCG	1 cut(s)	3775
HpyCH4IV	ACGT	6 cut(s)	1681, 1789, 3003, 3135, 3295, 3835
HpySE526I	ACGT	6 cut(s)	1681, 1789, 3003, 3135, 3295, 3835
Hsp92I	GRCGYC	1 cut(s)	3003
HspAI	GCGC	5 cut(s)	104, 1526, 2017, 2633, 3112
Ksp22I	TGATCA	2 cut(s)	235, 4155
KspAI	GTTAAC	1 cut(s)	1177
KspI	CCGCGG	1 cut(s)	113
LmnI	GCTCC	5 cut(s)	673, 786, 1725, 3284, 3821
Lsp1109I	GCAGC	9 cut(s)	15, 18, 118, 740, 1571, 1657, 2238, 2241, 2749
LweI	GCATC	7 cut(s)	283, 1450, 1576, 1759, 1894, 3163, 3528
MaeI	CTAG	7 cut(s)	1908, 2489, 2615, 3077, 3167, 3467, 3897
MaeII	ACGT	6 cut(s)	1681, 1789, 3003, 3135, 3295, 3835
MflI	RGATCY	7 cut(s)	1317, 1510, 1830, 2140, 2331, 2371, 3961
MhlI	GDGCHC	2 cut(s)	783, 1192
MlsI	TGGCCA	1 cut(s)	2338
MluNI	TGGCCA	1 cut(s)	2338
MlyI	GAGTC	7 cut(s)	1582, 1701, 2156, 3059, 3389, 3761, 3866
MmeI	TCCRAC	7 cut(s)	1200, 1765, 2182, 2839, 3316, 3753, 3799
Mox20I	TGGCCA	1 cut(s)	2338
Mph1103I	ATGCAT	2 cut(s)	1545, 3733
MroXI	GAANNNNTTC	3 cut(s)	1352, 2958, 3381
MscI	TGGCCA	1 cut(s)	2338
MslI	CAYNNNNRTG	4 cut(s)	366, 417, 2133, 2480
Msp20I	TGGCCA	1 cut(s)	2338
MspA1I	CMGCKG	3 cut(s)	112, 2762, 3883
MspI	CCGG	3 cut(s)	913, 1209, 2035
MspR9I	CCNGG	9 cut(s)	306, 760, 1247, 2036, 2247, 2498, 2630, 3564, 3831
Mva1269I	GAATGC	3 cut(s)	54, 1368, 3558
MvaI	CCWGG	8 cut(s)	306, 760, 1247, 2247, 2498, 2630, 3564, 3831
MvnI	CGCG	3 cut(s)	112, 3053, 3114
NciI	CCSGG	1 cut(s)	2036
NcoI	CCATGG	1 cut(s)	171
NlaIV	GGNNCC	3 cut(s)	424, 475, 1224
NmeAIII	GCCGAG	1 cut(s)	3811
NmuCI	GTSAC	3 cut(s)	410, 1117, 1958
NruI	TCGCGA	1 cut(s)	3053
NsiI	ATGCAT	2 cut(s)	1545, 3733
NspI	RCATGY	3 cut(s)	2276, 2569, 3060
NspV	TTCGAA	1 cut(s)	3626
OliI	CACNNNNGTG	1 cut(s)	2133
PagI	TCATGA	2 cut(s)	823, 3676
PasI	CCCWGGG	1 cut(s)	1246
PceI	AGGCCT	1 cut(s)	3987
PciI	ACATGT	3 cut(s)	2272, 2565, 3056
PctI	GAATGC	3 cut(s)	54, 1368, 3558
PdmI	GAANNNNTTC	3 cut(s)	1352, 2958, 3381
PfoI	TCCNGGA	1 cut(s)	3829
PleI	GAGTC	7 cut(s)	1581, 1701, 2155, 3059, 3389, 3761, 3866
PmaCI	CACGTG	1 cut(s)	1682
PmlI	CACGTG	1 cut(s)	1682
PpsI	GAGTC	7 cut(s)	1581, 1701, 2155, 3059, 3389, 3761, 3866
Ppu21I	YACGTR	2 cut(s)	1682, 3296
PpuMI	RGGWCCY	2 cut(s)	1102, 2524
PscI	ACATGT	3 cut(s)	2272, 2565, 3056
PshAI	GACNNNNGTC	1 cut(s)	1124
PsiI	TTATAA	1 cut(s)	3920
Psp124BI	GAGCTC	1 cut(s)	783
Psp5II	RGGWCCY	2 cut(s)	1102, 2524
Psp6I	CCWGG	8 cut(s)	304, 758, 1245, 2245, 2496, 2628, 3562, 3829
PspCI	CACGTG	1 cut(s)	1682
PspEI	GGTNACC	1 cut(s)	3890
PspFI	CCCAGC	3 cut(s)	100, 2450, 3205
PspGI	CCWGG	8 cut(s)	304, 758, 1245, 2245, 2496, 2628, 3562, 3829
PspN4I	GGNNCC	3 cut(s)	424, 475, 1224
PspPI	GGNCC	7 cut(s)	78, 473, 1102, 1206, 2453, 2524, 3927
PspPPI	RGGWCCY	2 cut(s)	1102, 2524
PstI	CTGCAG	1 cut(s)	33
PstNI	CAGNNNCTG	2 cut(s)	635, 2978
PsuI	RGATCY	7 cut(s)	1317, 1510, 1830, 2140, 2331, 2371, 3961
PvuII	CAGCTG	2 cut(s)	2762, 3883
RruI	TCGCGA	1 cut(s)	3053
RsaI	GTAC	9 cut(s)	90, 213, 722, 1113, 1239, 1480, 2208, 2471, 2687
RsaNI	GTAC	9 cut(s)	89, 212, 721, 1112, 1238, 1479, 2207, 2470, 2686
RseI	CAYNNNNRTG	4 cut(s)	366, 417, 2133, 2480
SacI	GAGCTC	1 cut(s)	783
SacII	CCGCGG	1 cut(s)	113
Sau96I	GGNCC	7 cut(s)	78, 473, 1102, 1206, 2453, 2524, 3927
SchI	GAGTC	7 cut(s)	1582, 1701, 2156, 3059, 3389, 3761, 3866
ScrFI	CCNGG	9 cut(s)	306, 760, 1247, 2036, 2247, 2498, 2630, 3564, 3831
SduI	GDGCHC	2 cut(s)	783, 1192
SfaNI	GCATC	7 cut(s)	283, 1450, 1576, 1759, 1894, 3163, 3528
SfcI	CTRYAG	4 cut(s)	29, 522, 892, 963
Sfr303I	CCGCGG	1 cut(s)	113
SfuI	TTCGAA	1 cut(s)	3626
SgrBI	CCGCGG	1 cut(s)	113
SinI	GGWCC	4 cut(s)	473, 1102, 2524, 3927
SmiI	ATTTAAAT	1 cut(s)	3192
SmiMI	CAYNNNNRTG	4 cut(s)	366, 417, 2133, 2480
SmlI	CTYRAG	4 cut(s)	834, 2107, 2144, 2315
SmoI	CTYRAG	4 cut(s)	834, 2107, 2144, 2315
SseBI	AGGCCT	1 cut(s)	3987
SsiI	CCGC	6 cut(s)	110, 112, 342, 576, 864, 2626
SspI	AATATT	2 cut(s)	3073, 3301
SspMI	CTAG	7 cut(s)	1908, 2489, 2615, 3077, 3167, 3467, 3897
SstI	GAGCTC	1 cut(s)	783
StuI	AGGCCT	1 cut(s)	3987
StyD4I	CCNGG	9 cut(s)	304, 758, 1245, 2034, 2245, 2496, 2628, 3562, 3829
StyI	CCWWGG	6 cut(s)	171, 858, 1035, 1405, 2689, 3557
SwaI	ATTTAAAT	1 cut(s)	3192
TaiI	ACGT	6 cut(s)	1684, 1792, 3006, 3138, 3298, 3838
TaqI	TCGA	8 cut(s)	495, 614, 1753, 1822, 2788, 3478, 3626, 4116
TatI	WGTACW	5 cut(s)	88, 211, 1237, 2206, 2469
TauI	GCSGC	3 cut(s)	112, 579, 866
TscAI	CASTG	5 cut(s)	2182, 2594, 2896, 3216, 4003
TseFI	GTSAC	3 cut(s)	410, 1117, 1958
TseI	GCWGC	9 cut(s)	6, 28, 106, 728, 1559, 1645, 2251, 2254, 2762
Tsp45I	GTSAC	3 cut(s)	410, 1117, 1958
TspGWI	ACGGA	3 cut(s)	236, 1780, 2738
TspRI	CASTG	5 cut(s)	2182, 2594, 2896, 3216, 4003
VpaK11BI	GGWCC	4 cut(s)	473, 1102, 2524, 3927
XagI	CCTNNNNNAGG	1 cut(s)	2303
XapI	RAATTY	5 cut(s)	651, 1818, 2958, 3339, 4080
XbaI	TCTAGA	2 cut(s)	2488, 2614
XceI	RCATGY	3 cut(s)	2276, 2569, 3060
XcmI	CCANNNNNNNNNTGG	1 cut(s)	169
XmiI	GTMKAC	5 cut(s)	311, 695, 891, 1778, 2907
XmnI	GAANNNNTTC	3 cut(s)	1352, 2958, 3381
XspI	CTAG	7 cut(s)	1908, 2489, 2615, 3077, 3167, 3467, 3897
ZraI	GACGTC	1 cut(s)	3004
Zsp2I	ATGCAT	2 cut(s)	1545, 3733

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.