Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0005249

Physical Location & Seq

Genomic Coordinates Forward (+)

6850815 .. 6861543

Gene Structure 10729 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1096800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4218 bp

ATGAGCAATTCCGTAGGCCATAACTTGCTCAACCAGAGCCTGCTTCAAAGAAAGGTAAATTCTTCCGGTATTCCTGCCAACACATTGTTTCAAGCTAAGAGCGTCCACCAAGTGACGGCACAGGCGCGAAAATCATCGATATCCAAGAAGTTTTGTGGGAACACTTTGAACGTTCAGAAACCAAAGTTAGCCATGGGGTCACGCCGCCCCGTTGCAGCTGTTCCTCGTGCCGTATTAACCACTAATCCGCCTTCGGATCAGCAGCTTGCCGGGAAATTCAATCTCGATGGGAATATTGAATTGCAGGTTTATGTGAATGCTTCCACTCCGGGATCTGCCACTCAAGTAAATTTTCTTGTAGCATACAGCGGTGACTCTTTGACTCTTCACTGGGGTGGGGTACAAGATATGAAAGAAAAGTGGGTGCTTCCGTCTCGTCACCCAGATGGAACCAAAGTATACAAGAACAGAGCTCTTAGAACACCTTTTGTGAGTTCTGGCTCCAGTTCCTCGCTTCAAATTGAGATTGATGATCCTACAATACAAGCAATCGAGTTTCTTTTAGTTGATGAAAGCCAGAACAGATGGTTTAAGAACAATGGTGGTAACTTTCATGTTAAATTACCTGCGAAAGAGAAGCTGATATCCAATGCTCCAGTTCCTGAAGAGCTTGTACAAATTCAAGCATATTTGAGGTGGGAAAGAAAGGGTAAACAGATGTATACACCAGAGCAAGAAAAGGTGGAGTACGAAGCAGCCCGATCTGAGTTATTGGAGGAAGTAGCTAGGGGTACTTCCATACAAGATCTCCATGCAAGGTTGACTAAGAAAGATGATGATGGTAAAATTGAAGAGCCATCTCGTTCTGATACAAAGACTGCAAAGAAAGATGAGAGAAAACAGGTTTTTAGAACCAAGGGAATTCAACGCAAGAGGAGGGACTTAATGCAGATTATCAACAAACATGCTTCTAAACCTGTTTACGAGGGTAAAATTGTAGATGAAGAACATCCAGCCAAACCAAAACCCTTGACAGCAGTTGAGCTTTTCGCCAAGGAAAAGGAAGAACAGGATGGAGGTTCTATTCTGAGGAAAAACATTTTCAAGCTCAATGATAAGGAACTTCTGATACTCGTAACCAATCCTGCTGGGAAGACAAAGGTTCATCTGGCAACAGATTTCAAAGAGCCACTTACCCTTCACTGGGCTTTATCTGCAAGCAAGGCTGGAGAGTGGTTGGAACCACCTGCAAACACACTTCCCCAGGGTTCAGTTGCTCTTAATGGGGTTACTGAAACACAATTTAGACCAGGTTCCGCTGACTCTACTTATGAGGTTCAATCTTTAGAAATAGAAGTTGAAGCAGATAATTTTAAAGGAATGCCATTCGTCCTTAATGCTTCTGGGACATGGATGAAGAATCAAGGCTCCAATTTCTATGTTGACTTTGGCGTTGAACGCAAGAAAGCTCAGAAGGATACTGGTGATGGCAAAGGTACTGCAAAGGCTTTGTTGGATAAAATAGCAGGCCAGGAGAGTGAGGCACAGAAATCTTTTATGCATCGATTTAACATTGCAGCAGACTTGGTCAATCAAGCAAAGGATGCTGGCGAATTAGGTCTTGCGGGTATTTTGGTGTGGATGAGGTTTATGGCCACAAGGCAGCTCATTTGGAATAAAAATTACAATGTGAAACCACGTGAGATTAGTAAAGCTCAGAACAGGCTCACAGACTTGCTTCAGAATGTTTATGCAAATTATCCTCAGTATCGAGAACTTCTACGGATGATATTGTCTACTGTTGGCCGTGGAGGAGAAGGGGATGTGGGACAGCGAATTCGAGATGAGATCCTTGTCGTCCAGAGAAACAATGATTGCAATGGCTCATTTATGGAGGAATGGCATCAAAAGTTGCATAATAACACGAGTCCAGATGATGTTGTTATTTGTCAGGCATTAATGGATTACATAAAAAATGACTTTGATATTGGTGTTTACTGGAAAACTTTGAATGAGAATGGAATCACAAAAGAACGTCTTCTAAGCTACGATCGTGCAATCCATAATGAACCAAAATTCAGGAGAGATCAGAAAGAGGGTCTTCTGCGGGATCTGGGGCATTACATGAGAACTTTGAAGGCTGTTCATTCAGGTGCAGATCTTGAGTCTGCCATTCAAAATTGTATGGGCTACAAAGATGAGGGTCAAGGATTCATGGTTGGAGTAAAAATAAATCCTATATCTGGCTTGCCATCAGAAGTTCCAGATCTCCTGCGATTTGTACTAGAACATGTTGAAGACAGGAATGTGGAAGTCCTTGTTGAGGGTTTGCTTGAGGCGCGCCAGGGGCTTAGGACATTGCTCTCTAAGCCTCATGATCGTCTGAGGGATCTTTTATTTTTGGACATTGCCCTTGATTCTACTGTTAGGACCGCTATTGAGAGGGGATATGAAGAACTGAATAATGCTGGGCCAGAGAAAATTATGTACTTCATCTCTTTGGTTCTGGAAAACCTGGCACTGTCATCAGATGATAACGAGGATCTCATTTACTGTTTGAAGGGATGGGATCAGGCTCAAAACATGTTAAAAAGTAACAGTAATGACTGGGCATTATATTCAAAATCAATCCTAGACAGAACCCGCCTTGCGCTTGCATACAAGGCAGAATCATATCTCAGTATTTTACAGCCGTCAGCAGAATACCTTGGAGCACAGCTTGGAGTGGACCAATGGGCTTTGAACATATTTACCGAAGAAATAATCCGTGCTGGATCAGCTGCCTCTTTATCCTCACTCCTTAATCGGCTTGATCCGGTTCTTCGAAAGACTGCTCATATGGGAAGTTGGCAGGTTATCAGCCCAATTGAAGTGGTTGGATATGTGGTCTATGTGGATGAATTACTCACTGTTCAGAATAAAGTTTACAGCAAGCCCACAATTTTGGTGGCAAAAACTGTAAAGGGTGAGGAAGAAATTCCAGATGGTACTGTTGCAGTGCTGACACCTGATATGCCAGACGTCCTATCTCATGTTTCTGTCCGAGCAAGAAATAGCAAGGTATGTTTTGCGACATGCTTTGACCCCAATATTCTGGCCGACCTCAAAGCTAGTGAAGGGAAGTTATTACGTCTAAAGCCAACACCTGCTGATATAGTTTATAGTGAGGTCAATGAGGGTGAGCTAGATGCGAGTTCAACTGATTCAAGAGAAGACACTCCATCTCTAACATTGGTCAAGAAAGAATTTACTGGTAGATATGCCATATCATCTGATGAGTTCACAAGTGAAATGGTTGGAGCTAAATCACGTAATATTGCTTATGTAAAAGGAAAACTTCCGTCTTGGGTTGGAATTCCTACATCAGTTGCCCTACCATTTGGATCATTCGAAAAGGTTCTTTCAGAAGATTCGAATAAGGCTGTGGCTGAAAAGTTGGAAACTCTAAAGAAAAAGTTAAGGGACGGAAATTTTGATTCACTCAAGGAGATTCGGGAAACAGTTTTACAGATGGCAGCACCACCCCAGTTGGTGCAAGAGCTGAAGACTACGATGAAAAGTTCAGGAATGCCTTGGCCTGGTGATGAAGGCGAACAACGGTGGGAACAAGCATGGACGGCTATAAAAAAGGTCTGGGCTTCAAAGTGGAACGAGAGAGCATACTTCAGCACGAGAAAAGTGAAACTAGATCACGAGTATCTCTGCATGGCTGTCCTTGTTCAGGAAATCATAAATGCTGACTATGCATTCGTTATTCATACAACTAACCCATCTTCAGGAGACTCATCAGAGATATATGCTGAGGTTGTTAAGGGACTTGGAGAGACTCTAGTTGGAGCTTATCCTGGACGTGCTCTAAGTTTTATTAGCAAAAAGAATGATCTGGACTCTCCTCAGGTGTTGGGTTACCCTAGCAAACCCATCGGCCTCTTTATAAGACGATCCATAATCTTTCGATCTGATTCCAATGGTGAAGATCTCGAAGGTTATGCTGGTGCAGGCCTGTATGACAGTGTCCCTATGGACGAGGAAGAGAAAGTCTTGGTCGACTACTCATCTGACCCGCTTATGGTTGACGGAAACTTTCGCAAGACAATCCTCTCTAGCATTGCTCGTGCAGGAAGTGCAATCGAGGAACTGTATGGATCTCCACAAGACATTGAAGGCGTGATCAGGGACGGTAAACTCTATGTTGTCCAGACAAGACCCCAAATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009628 GO:0009631 GO:0009941 GO:0009987 GO:0016052 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016781 GO:0031967 GO:0031975 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050521 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPDK_N

Protein Analysis

1406

Amino Acids

156.91

Weight (kDa)

6.17

Isoelectric Point (pI)

39.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ig_N_CWD1	PF23166	90 - 208	7e-40	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
DUF7067	PF23229	222 - 277	1.8e-27	Alpha-glucan water dikinase, DUF7067
Ig_N_CWD1	PF23166	366 - 483	5.9e-26	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
GWD1_pHisD	PF22973	949 - 1061	8.9e-53	Alpha-glucan water dikinase, phosphohistidine-like domain
PPDK_N	PF01326	1202 - 1404	4.7e-21	Pyruvate phosphate dikinase, AMP/ATP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0005249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10760 AT1G10760 AT1G10760
fragaria_vesca	FvH4_4g25800 FvH4_4g25800 FvH4_4g25800 FvH4_4g25800
malus_domestica	MD13G1096800.v1.1 MD16G1097800.v1.1
prunus_persica	Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1
pyrus_communis	pycom13g08450 pycom16g08330
rosa_chinensis	RchiOBHm_Chr4g0433251
rosa_laevigata	RLG00000006771
rosa_multiflora	Rmu_co8055492.1_g000001 Rmu_sc0010643.1_g000001
rosa_roxburghii	Rroxscaffold_5G00374810
rosa_rugosa	Rorug04G0267200 Rorug04G0267300 Rorug04G0267400 Rorug04G0267400
rosa_samantha	Rh4AG321900 Rh4BG329300 Rh4CG344600
rosa_wichuraiana	Rw4G027860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3935
AarI	CACCTGC	2 cut(s)	1255, 3154
AatII	GACGTC	1 cut(s)	3024
Acc36I	ACCTGC	5 cut(s)	295, 634, 1255, 2842, 3154
AccI	GTMKAC	4 cut(s)	459, 722, 1796, 4047
AccII	CGCG	2 cut(s)	127, 2341
AciI	CCGC	9 cut(s)	205, 248, 369, 1317, 1625, 2107, 2433, 2644, 4064
AclI	AACGTT	1 cut(s)	171
AcoI	YGGCCR	3 cut(s)	1653, 1804, 3096
AcuI	CTGAAG	5 cut(s)	684, 1724, 3563, 3649, 3759
AcvI	CACGTG	1 cut(s)	1700
AcyI	GRCGYC	1 cut(s)	3021
AdeI	CACNNNGTG	1 cut(s)	112
AfaI	GTAC	8 cut(s)	402, 675, 749, 793, 1498, 2283, 2489, 2989
AfiI	CCNNNNNNNGG	9 cut(s)	115, 1203, 1204, 2243, 2323, 3578, 3721, 3776, 4069
AflIII	ACRYGT	2 cut(s)	2290, 2583
AjiI	CACGTC	1 cut(s)	3851
AjnI	CCWGG	7 cut(s)	1263, 1309, 1530, 2343, 2514, 3577, 3844
AleI	CACNNNNGTG	1 cut(s)	393
Alw21I	GWGCWC	3 cut(s)	475, 2716, 3856
Alw26I	GTCTC	3 cut(s)	438, 3774, 3818
AlwNI	CAGNNNCTG	2 cut(s)	40, 662
AoxI	GGCC	9 cut(s)	16, 1528, 1653, 1804, 2471, 3096, 3575, 3925, 4000
ApeKI	GCWGC	7 cut(s)	215, 262, 755, 1577, 1663, 2780, 3515
ArsI	GACNNNNNNTTYG	6 cut(s)	1821, 1853, 3455, 3487, 3731, 3763
AscI	GGCGCGCC	1 cut(s)	2339
AseI	ATTAAT	1 cut(s)	1958
Asp700I	GAANNNNTTC	3 cut(s)	2037, 2976, 3396
AspLEI	GCGC	4 cut(s)	127, 2341, 2343, 2653
AspS9I	GGNCC	3 cut(s)	2430, 2471, 2728
AsuC2I	CCSGG	2 cut(s)	271, 330
AsuHPI	GGTGA	7 cut(s)	383, 431, 1496, 2978, 3191, 3593, 3983
AsuII	TTCGAA	3 cut(s)	2824, 3390, 3413
AvaII	GGWCC	2 cut(s)	2430, 2728
AxyI	CCTNAGG	1 cut(s)	3894
BalI	TGGCCA	1 cut(s)	1655
BanII	GRGCYC	1 cut(s)	475
BarI	GAAGNNNNNNTAC	2 cut(s)	657, 689
BauI	CACGAG	5 cut(s)	225, 1924, 3670, 3692, 4113
BbrPI	CACGTG	1 cut(s)	1700
BbsI	GAAGAC	6 cut(s)	1160, 2030, 2093, 2304, 3219, 3551
Bbv12I	GWGCWC	3 cut(s)	475, 2716, 3856
BbvCI	CCTCAGC	1 cut(s)	3801
BbvI	GCAGC	7 cut(s)	227, 274, 767, 1589, 1675, 2767, 3527
BceAI	ACGGC	5 cut(s)	132, 215, 1791, 2677, 3633
BcgI	CGANNNNNNTGC	4 cut(s)	3904, 3938, 3971, 4005
BciT130I	CCWGG	7 cut(s)	1265, 1311, 1532, 2345, 2516, 3579, 3846
BciVI	GTATCC	1 cut(s)	1471
BclI	TGATCA	1 cut(s)	4170
BcnI	CCSGG	2 cut(s)	271, 330
BcoDI	GTCTC	3 cut(s)	438, 3774, 3818
BfaI	CTAG	9 cut(s)	786, 2285, 2633, 3111, 3185, 3686, 3830, 3912, 4104
BfuAI	ACCTGC	5 cut(s)	295, 634, 1255, 2842, 3154
BfuI	GTATCC	1 cut(s)	1471
BglII	AGATCT	4 cut(s)	805, 2158, 2266, 3976
BisI	GCNGC	8 cut(s)	205, 216, 263, 756, 1578, 1664, 2781, 3516
BlsI	GCNGC	8 cut(s)	206, 217, 264, 757, 1579, 1665, 2782, 3517
Bme1390I	CCNGG	9 cut(s)	271, 330, 1265, 1311, 1532, 2345, 2516, 3579, 3846
Bme18I	GGWCC	2 cut(s)	2430, 2728
BmgBI	CACGTC	1 cut(s)	3851
BmgT120I	GGNCC	3 cut(s)	2430, 2471, 2728
BmiI	GGNNCC	5 cut(s)	451, 502, 1242, 1315, 1429
BmrFI	CCNGG	9 cut(s)	271, 330, 1265, 1311, 1532, 2345, 2516, 3579, 3846
BmrI	ACTGGG	4 cut(s)	400, 1213, 2617, 3520
BmsI	GCATC	4 cut(s)	1570, 1594, 1912, 3178
BmuI	ACTGGG	4 cut(s)	400, 1213, 2617, 3520
BpiI	GAAGAC	6 cut(s)	1160, 2030, 2093, 2304, 3219, 3551
BpmI	CTGGAG	3 cut(s)	487, 639, 1248
Bpu10I	CCTNAGC	2 cut(s)	2351, 3801
Bpu14I	TTCGAA	3 cut(s)	2824, 3390, 3413
BpuEI	CTTGAG	4 cut(s)	327, 2183, 2354, 3467
BpuMI	CCSGG	2 cut(s)	271, 330
Bsa29I	ATCGAT	2 cut(s)	137, 1564
BsaAI	YACGTR	2 cut(s)	1700, 3311
BsaHI	GRCGYC	1 cut(s)	3021
BsaJI	CCNNGG	9 cut(s)	192, 915, 1053, 1263, 1264, 1807, 2344, 2707, 3572
BsaWI	WCCGGW	2 cut(s)	65, 2815
BsaXI	ACNNNNNCTCC	2 cut(s)	792, 822
Bsc4I	CCNNNNNNNGG	9 cut(s)	115, 1203, 1204, 2243, 2323, 3578, 3721, 3776, 4069
Bse1I	ACTGG	9 cut(s)	395, 504, 656, 1208, 1486, 2003, 2612, 3256, 3526
Bse21I	CCTNAGG	1 cut(s)	3894
Bse3DI	GCAATG	5 cut(s)	1572, 1885, 2357, 2406, 4107
BseBI	CCWGG	7 cut(s)	1265, 1311, 1532, 2345, 2516, 3579, 3846
BseCI	ATCGAT	2 cut(s)	137, 1564
BseDI	CCNNGG	9 cut(s)	192, 915, 1053, 1263, 1264, 1807, 2344, 2707, 3572
BseGI	GGATG	9 cut(s)	1009, 1078, 1419, 1609, 1647, 1791, 1828, 2570, 2902
BseLI	CCNNNNNNNGG	9 cut(s)	115, 1203, 1204, 2243, 2323, 3578, 3721, 3776, 4069
BseMI	GCAATG	5 cut(s)	1572, 1885, 2357, 2406, 4107
BseMII	CTCAG	9 cut(s)	756, 1079, 1484, 1730, 1778, 2375, 2692, 3792, 3908
BseNI	ACTGG	9 cut(s)	395, 504, 656, 1208, 1486, 2003, 2612, 3256, 3526
BsePI	GCGCGC	1 cut(s)	2339
BseRI	GAGGAG	3 cut(s)	949, 1827, 3882
BseXI	GCAGC	7 cut(s)	227, 274, 767, 1589, 1675, 2767, 3527
BseYI	CCCAGC	2 cut(s)	1148, 2468
BsgI	GTGCAG	3 cut(s)	2175, 4017, 4137
Bsh1236I	CGCG	2 cut(s)	127, 2341
Bsh1285I	CGRYCG	1 cut(s)	2053
BshFI	GGCC	9 cut(s)	18, 1530, 1655, 1806, 2473, 3098, 3577, 3927, 4002
BshVI	ATCGAT	2 cut(s)	137, 1564
BsiEI	CGRYCG	1 cut(s)	2053
BsiHKAI	GWGCWC	3 cut(s)	475, 2716, 3856
BsiSI	CCGG	4 cut(s)	66, 270, 329, 2816
BslFI	GGGAC	7 cut(s)	953, 1420, 1842, 3476, 3828, 4001, 4190
BslI	CCNNNNNNNGG	9 cut(s)	115, 1203, 1204, 2243, 2323, 3578, 3721, 3776, 4069
BsmAI	GTCTC	3 cut(s)	438, 3774, 3818
BsmBI	CGTCTC	1 cut(s)	438
BsmFI	GGGAC	7 cut(s)	953, 1420, 1842, 3476, 3828, 4001, 4190
BsmI	GAATGC	4 cut(s)	322, 1386, 3573, 3746
BsnI	GGCC	9 cut(s)	18, 1530, 1655, 1806, 2473, 3098, 3577, 3927, 4002
Bsp119I	TTCGAA	3 cut(s)	2824, 3390, 3413
Bsp1286I	GDGCHC	3 cut(s)	475, 2716, 3856
Bsp1407I	TGTACA	1 cut(s)	673
Bsp19I	CCATGG	1 cut(s)	192
BspACI	CCGC	9 cut(s)	205, 248, 369, 1317, 1625, 2107, 2433, 2644, 4064
BspANI	GGCC	9 cut(s)	18, 1530, 1655, 1806, 2473, 3098, 3577, 3927, 4002
BspCNI	CTCAG	9 cut(s)	757, 1080, 1483, 1729, 1777, 2376, 2691, 3793, 3907
BspDI	ATCGAT	2 cut(s)	137, 1564
BspFNI	CGCG	2 cut(s)	127, 2341
BspHI	TCATGA	1 cut(s)	2374
BspLI	GGNNCC	5 cut(s)	451, 502, 1242, 1315, 1429
BspMI	ACCTGC	5 cut(s)	295, 634, 1255, 2842, 3154
BspQI	GCTCTTC	2 cut(s)	660, 846
BspT104I	TTCGAA	3 cut(s)	2824, 3390, 3413
BsrDI	GCAATG	5 cut(s)	1572, 1885, 2357, 2406, 4107
BsrGI	TGTACA	1 cut(s)	673
BsrI	ACTGG	9 cut(s)	395, 504, 656, 1208, 1486, 2003, 2612, 3256, 3526
BssECI	CCNNGG	9 cut(s)	192, 915, 1053, 1263, 1264, 1807, 2344, 2707, 3572
BssHII	GCGCGC	1 cut(s)	2339
BssNAI	GTATAC	2 cut(s)	460, 723
BssNI	GRCGYC	1 cut(s)	3021
BssSI	CACGAG	5 cut(s)	225, 1924, 3670, 3692, 4113
BssT1I	CCWWGG	5 cut(s)	192, 915, 1053, 2707, 3572
Bst1107I	GTATAC	2 cut(s)	460, 723
Bst2BI	CACGAG	5 cut(s)	225, 1924, 3670, 3692, 4113
Bst2UI	CCWGG	7 cut(s)	1265, 1311, 1532, 2345, 2516, 3579, 3846
Bst6I	CTCTTC	4 cut(s)	390, 660, 846, 4026
BstACI	GRCGYC	1 cut(s)	3021
BstAPI	GCANNNNNTGC	2 cut(s)	1604, 4124
BstAUI	TGTACA	1 cut(s)	673
BstBAI	YACGTR	2 cut(s)	1700, 3311
BstBI	TTCGAA	3 cut(s)	2824, 3390, 3413
BstDSI	CCRYGG	2 cut(s)	192, 1807
BstEII	GGTNACC	1 cut(s)	3905
BstENI	CCTNNNNNAGG	2 cut(s)	2321, 3719
BstF5I	GGATG	9 cut(s)	1009, 1078, 1419, 1609, 1647, 1791, 1828, 2570, 2902
BstFNI	CGCG	2 cut(s)	127, 2341
BstHHI	GCGC	4 cut(s)	127, 2341, 2343, 2653
BstMAI	GTCTC	3 cut(s)	438, 3774, 3818
BstMCI	CGRYCG	1 cut(s)	2053
BstNI	CCWGG	7 cut(s)	1265, 1311, 1532, 2345, 2516, 3579, 3846
BstNSI	RCATGY	4 cut(s)	968, 2294, 2587, 3078
BstPI	GGTNACC	1 cut(s)	3905
BstSCI	CCNGG	9 cut(s)	269, 328, 1263, 1309, 1530, 2343, 2514, 3577, 3844
BstUI	CGCG	2 cut(s)	127, 2341
BstV1I	GCAGC	7 cut(s)	227, 274, 767, 1589, 1675, 2767, 3527
BstV2I	GAAGAC	6 cut(s)	1160, 2030, 2093, 2304, 3219, 3551
BstXI	CCANNNNNNTGG	2 cut(s)	2944, 3094
BstZ17I	GTATAC	2 cut(s)	460, 723
Bsu15I	ATCGAT	2 cut(s)	137, 1564
Bsu36I	CCTNAGG	1 cut(s)	3894
BsuI	GTATCC	1 cut(s)	1471
BsuRI	GGCC	9 cut(s)	18, 1530, 1655, 1806, 2473, 3098, 3577, 3927, 4002
BsuTUI	ATCGAT	2 cut(s)	137, 1564
BtgI	CCRYGG	2 cut(s)	192, 1807
BtrI	CACGTC	1 cut(s)	3851
BtsCI	GGATG	9 cut(s)	1009, 1078, 1419, 1609, 1647, 1791, 1828, 2570, 2902
BtsI	GCAGTG	1 cut(s)	3003
BtsIMutI	CAGTG	6 cut(s)	388, 1201, 2519, 2907, 3003, 4018
BveI	ACCTGC	5 cut(s)	295, 634, 1255, 2842, 3154
CaiI	CAGNNNCTG	2 cut(s)	40, 662
CciI	TCATGA	1 cut(s)	2374
CfoI	GCGC	4 cut(s)	127, 2341, 2343, 2653
Cfr13I	GGNCC	3 cut(s)	2430, 2471, 2728
ClaI	ATCGAT	2 cut(s)	137, 1564
CseI	GACGC	1 cut(s)	91
CsiI	ACCWGGT	1 cut(s)	1309
Csp6I	GTAC	8 cut(s)	401, 674, 748, 792, 1497, 2282, 2488, 2988
CspCI	CAANNNNNGTGG	6 cut(s)	2853, 2888, 2928, 2963, 3510, 3545
CviQI	GTAC	8 cut(s)	401, 674, 748, 792, 1497, 2282, 2488, 2988
DraI	TTTAAA	1 cut(s)	1375
DraIII	CACNNNGTG	1 cut(s)	112
EaeI	YGGCCR	3 cut(s)	1653, 1804, 3096
Eam1104I	CTCTTC	4 cut(s)	390, 660, 846, 4026
EarI	CTCTTC	4 cut(s)	390, 660, 846, 4026
EciI	GGCGGA	1 cut(s)	237
Ecl136II	GAGCTC	1 cut(s)	473
Eco130I	CCWWGG	5 cut(s)	192, 915, 1053, 2707, 3572
Eco147I	AGGCCT	1 cut(s)	4002
Eco24I	GRGCYC	1 cut(s)	475
Eco32I	GATATC	2 cut(s)	141, 645
Eco47I	GGWCC	2 cut(s)	2430, 2728
Eco53kI	GAGCTC	1 cut(s)	473
Eco57I	CTGAAG	5 cut(s)	684, 1724, 3563, 3649, 3759
Eco72I	CACGTG	1 cut(s)	1700
Eco81I	CCTNAGG	1 cut(s)	3894
Eco91I	GGTNACC	1 cut(s)	3905
EcoICRI	GAGCTC	1 cut(s)	473
EcoNI	CCTNNNNNAGG	2 cut(s)	2321, 3719
EcoO65I	GGTNACC	1 cut(s)	3905
EcoRI	GAATTC	3 cut(s)	921, 1836, 3354
EcoRII	CCWGG	7 cut(s)	1263, 1309, 1530, 2343, 2514, 3577, 3844
EcoRV	GATATC	2 cut(s)	141, 645
EcoT14I	CCWWGG	5 cut(s)	192, 915, 1053, 2707, 3572
EcoT22I	ATGCAT	2 cut(s)	1563, 3748
EcoT38I	GRGCYC	1 cut(s)	475
ErhI	CCWWGG	5 cut(s)	192, 915, 1053, 2707, 3572
Esp3I	CGTCTC	1 cut(s)	438
FalI	AAGNNNNNCTT	8 cut(s)	1176, 1208, 2022, 2054, 2085, 2117, 3321, 3353
FaqI	GGGAC	7 cut(s)	953, 1420, 1842, 3476, 3828, 4001, 4190
FauI	CCCGC	4 cut(s)	1618, 2100, 2651, 4071
FauNDI	CATATG	1 cut(s)	2838
FbaI	TGATCA	1 cut(s)	4170
FblI	GTMKAC	4 cut(s)	459, 722, 1796, 4047
Fnu4HI	GCNGC	8 cut(s)	205, 216, 263, 756, 1578, 1664, 2781, 3516
FokI	GGATG	9 cut(s)	996, 1085, 1426, 1616, 1654, 1798, 1835, 2577, 2909
FriOI	GRGCYC	1 cut(s)	475
Fsp4HI	GCNGC	8 cut(s)	205, 216, 263, 756, 1578, 1664, 2781, 3516
FspBI	CTAG	9 cut(s)	786, 2285, 2633, 3111, 3185, 3686, 3830, 3912, 4104
GlaI	GCGC	4 cut(s)	126, 2340, 2342, 2652
GluI	GCNGC	8 cut(s)	205, 216, 263, 756, 1578, 1664, 2781, 3516
GsaI	CCCAGC	2 cut(s)	1152, 2472
GsuI	CTGGAG	3 cut(s)	487, 639, 1248
HaeIII	GGCC	9 cut(s)	18, 1530, 1655, 1806, 2473, 3098, 3577, 3927, 4002
HapII	CCGG	4 cut(s)	66, 270, 329, 2816
HgaI	GACGC	1 cut(s)	91
HhaI	GCGC	4 cut(s)	127, 2341, 2343, 2653
Hin1I	GRCGYC	1 cut(s)	3021
Hin6I	GCGC	4 cut(s)	125, 2339, 2341, 2651
HinP1I	GCGC	4 cut(s)	125, 2339, 2341, 2651
HincII	GTYRAC	4 cut(s)	822, 1444, 4048, 4075
HindII	GTYRAC	4 cut(s)	822, 1444, 4048, 4075
HpaII	CCGG	4 cut(s)	66, 270, 329, 2816
HphI	GGTGA	7 cut(s)	383, 431, 1496, 2978, 3191, 3593, 3983
HpyCH4IV	ACGT	7 cut(s)	171, 1699, 2035, 3021, 3130, 3310, 3850
HpySE526I	ACGT	7 cut(s)	171, 1699, 2035, 3021, 3130, 3310, 3850
Hsp92I	GRCGYC	1 cut(s)	3021
HspAI	GCGC	4 cut(s)	125, 2339, 2341, 2651
Ksp22I	TGATCA	1 cut(s)	4170
LguI	GCTCTTC	2 cut(s)	660, 846
LmnI	GCTCC	6 cut(s)	506, 658, 1433, 2711, 3299, 3836
Lsp1109I	GCAGC	7 cut(s)	227, 274, 767, 1589, 1675, 2767, 3527
LweI	GCATC	4 cut(s)	1570, 1594, 1912, 3178
MabI	ACCWGGT	1 cut(s)	1309
MaeI	CTAG	9 cut(s)	786, 2285, 2633, 3111, 3185, 3686, 3830, 3912, 4104
MaeII	ACGT	7 cut(s)	171, 1699, 2035, 3021, 3130, 3310, 3850
MaeIII	GTNAC	9 cut(s)	112, 198, 371, 437, 605, 1135, 1288, 2594, 3905
MfeI	CAATTG	1 cut(s)	2865
MhlI	GDGCHC	3 cut(s)	475, 2716, 3856
MlsI	TGGCCA	1 cut(s)	1655
MluNI	TGGCCA	1 cut(s)	1655
MlyI	GAGTC	8 cut(s)	368, 376, 1316, 1936, 2174, 3776, 3820, 3881
MmeI	TCCRAC	8 cut(s)	1218, 1494, 2200, 2857, 3277, 3331, 3417, 3814
Mox20I	TGGCCA	1 cut(s)	1655
Mph1103I	ATGCAT	2 cut(s)	1563, 3748
MroXI	GAANNNNTTC	3 cut(s)	2037, 2976, 3396
MscI	TGGCCA	1 cut(s)	1655
MslI	CAYNNNNRTG	3 cut(s)	393, 444, 2151
Msp20I	TGGCCA	1 cut(s)	1655
MspA1I	CMGCKG	4 cut(s)	218, 369, 1319, 2780
MspI	CCGG	4 cut(s)	66, 270, 329, 2816
MspR9I	CCNGG	9 cut(s)	271, 330, 1265, 1311, 1532, 2345, 2516, 3579, 3846
MunI	CAATTG	1 cut(s)	2865
Mva1269I	GAATGC	4 cut(s)	322, 1386, 3573, 3746
MvaI	CCWGG	7 cut(s)	1265, 1311, 1532, 2345, 2516, 3579, 3846
MvnI	CGCG	2 cut(s)	127, 2341
NciI	CCSGG	2 cut(s)	271, 330
NcoI	CCATGG	1 cut(s)	192
NdeI	CATATG	1 cut(s)	2838
NlaIV	GGNNCC	5 cut(s)	451, 502, 1242, 1315, 1429
NmuCI	GTSAC	4 cut(s)	112, 198, 371, 437
NsiI	ATGCAT	2 cut(s)	1563, 3748
NspI	RCATGY	4 cut(s)	968, 2294, 2587, 3078
NspV	TTCGAA	3 cut(s)	2824, 3390, 3413
OliI	CACNNNNGTG	1 cut(s)	393
PagI	TCATGA	1 cut(s)	2374
PalAI	GGCGCGCC	1 cut(s)	2339
PaqCI	CACCTGC	2 cut(s)	1255, 3154
PasI	CCCWGGG	1 cut(s)	1264
PauI	GCGCGC	1 cut(s)	2339
PceI	AGGCCT	1 cut(s)	4002
PciI	ACATGT	2 cut(s)	2290, 2583
PciSI	GCTCTTC	2 cut(s)	660, 846
PctI	GAATGC	4 cut(s)	322, 1386, 3573, 3746
PdmI	GAANNNNTTC	3 cut(s)	2037, 2976, 3396
PflFI	GACNNNGTC	2 cut(s)	1586, 4013
PfoI	TCCNGGA	2 cut(s)	328, 3844
PkrI	GCNGC	8 cut(s)	206, 217, 264, 757, 1579, 1665, 2782, 3517
Ple19I	CGATCG	1 cut(s)	2053
PleI	GAGTC	8 cut(s)	368, 376, 1316, 1935, 2173, 3776, 3820, 3881
PmaCI	CACGTG	1 cut(s)	1700
PmlI	CACGTG	1 cut(s)	1700
PpsI	GAGTC	8 cut(s)	368, 376, 1316, 1935, 2173, 3776, 3820, 3881
Ppu21I	YACGTR	2 cut(s)	1700, 3311
PscI	ACATGT	2 cut(s)	2290, 2583
PshBI	ATTAAT	1 cut(s)	1958
PsiI	TTATAA	1 cut(s)	3935
Psp124BI	GAGCTC	1 cut(s)	475
Psp1406I	AACGTT	1 cut(s)	171
Psp6I	CCWGG	7 cut(s)	1263, 1309, 1530, 2343, 2514, 3577, 3844
PspCI	CACGTG	1 cut(s)	1700
PspEI	GGTNACC	1 cut(s)	3905
PspFI	CCCAGC	2 cut(s)	1148, 2468
PspGI	CCWGG	7 cut(s)	1263, 1309, 1530, 2343, 2514, 3577, 3844
PspN4I	GGNNCC	5 cut(s)	451, 502, 1242, 1315, 1429
PspPI	GGNCC	3 cut(s)	2430, 2471, 2728
PsrI	GAACNNNNNNTAC	2 cut(s)	1113, 1145
PstNI	CAGNNNCTG	2 cut(s)	40, 662
PsyI	GACNNNGTC	2 cut(s)	1586, 4013
PteI	GCGCGC	1 cut(s)	2339
PvuI	CGATCG	1 cut(s)	2053
PvuII	CAGCTG	2 cut(s)	218, 2780
RsaI	GTAC	8 cut(s)	402, 675, 749, 793, 1498, 2283, 2489, 2989
RsaNI	GTAC	8 cut(s)	401, 674, 748, 792, 1497, 2282, 2488, 2988
RseI	CAYNNNNRTG	3 cut(s)	393, 444, 2151
SacI	GAGCTC	1 cut(s)	475
SalI	GTCGAC	1 cut(s)	4046
SapI	GCTCTTC	2 cut(s)	660, 846
SatI	GCNGC	8 cut(s)	205, 216, 263, 756, 1578, 1664, 2781, 3516
Sau96I	GGNCC	3 cut(s)	2430, 2471, 2728
SchI	GAGTC	8 cut(s)	368, 376, 1316, 1936, 2174, 3776, 3820, 3881
ScrFI	CCNGG	9 cut(s)	271, 330, 1265, 1311, 1532, 2345, 2516, 3579, 3846
SduI	GDGCHC	3 cut(s)	475, 2716, 3856
SexAI	ACCWGGT	1 cut(s)	1309
SfaNI	GCATC	4 cut(s)	1570, 1594, 1912, 3178
SfuI	TTCGAA	3 cut(s)	2824, 3390, 3413
SgsI	GGCGCGCC	1 cut(s)	2339
SinI	GGWCC	2 cut(s)	2430, 2728
SmiMI	CAYNNNNRTG	3 cut(s)	393, 444, 2151
SmlI	CTYRAG	4 cut(s)	342, 2162, 2333, 3482
SmoI	CTYRAG	4 cut(s)	342, 2162, 2333, 3482
SseBI	AGGCCT	1 cut(s)	4002
SsiI	CCGC	9 cut(s)	205, 248, 369, 1317, 1625, 2107, 2433, 2644, 4064
SspI	AATATT	3 cut(s)	295, 3091, 3316
SspMI	CTAG	9 cut(s)	786, 2285, 2633, 3111, 3185, 3686, 3830, 3912, 4104
SstI	GAGCTC	1 cut(s)	475
StuI	AGGCCT	1 cut(s)	4002
StyD4I	CCNGG	9 cut(s)	269, 328, 1263, 1309, 1530, 2343, 2514, 3577, 3844
StyI	CCWWGG	5 cut(s)	192, 915, 1053, 2707, 3572
TaiI	ACGT	7 cut(s)	174, 1702, 2038, 3024, 3133, 3313, 3853
TatI	WGTACW	3 cut(s)	673, 2281, 2487
TauI	GCSGC	1 cut(s)	207
TscAI	CASTG	6 cut(s)	395, 1208, 2526, 2914, 3003, 4018
TseFI	GTSAC	4 cut(s)	112, 198, 371, 437
TseI	GCWGC	7 cut(s)	215, 262, 755, 1577, 1663, 2780, 3515
Tsp45I	GTSAC	4 cut(s)	112, 198, 371, 437
TspGWI	ACGGA	6 cut(s)	420, 1798, 2756, 3330, 3480, 4092
TspRI	CASTG	6 cut(s)	395, 1208, 2526, 2914, 3003, 4018
Tth111I	GACNNNGTC	2 cut(s)	1586, 4013
VpaK11BI	GGWCC	2 cut(s)	2430, 2728
VspI	ATTAAT	1 cut(s)	1958
XagI	CCTNNNNNAGG	2 cut(s)	2321, 3719
XceI	RCATGY	4 cut(s)	968, 2294, 2587, 3078
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2944
XmiI	GTMKAC	4 cut(s)	459, 722, 1796, 4047
XmnI	GAANNNNTTC	3 cut(s)	2037, 2976, 3396
XspI	CTAG	9 cut(s)	786, 2285, 2633, 3111, 3185, 3686, 3830, 3912, 4104
ZraI	GACGTC	1 cut(s)	3022
Zsp2I	ATGCAT	2 cut(s)	1563, 3748

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.