Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0005249

Physical Location & Seq

Genomic Coordinates Forward (+)

54592928 .. 54604440

Gene Structure 11513 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G027860.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3273 bp

ATGAAGATTCTCAACAAACATGCTACTAAACCTGTAGATGAAGCTAAACTTGCGCATGAAGAATCAAAGAAACCAAAAGCCTTGAAAGCAGTTGAGCTATTTGCCAGGGCAAAAGAAGAACAAGATGGTGGTTCCGTCCTGAACAAAAAAATCTACAGGCTCGGTGATAAGGACCTGCTGGTACTTGTGACAAAGGTTGCTGACAAGATAAAGGTTCATCTGGCAACAGATATCAAAGAGCCAATTAACATTCATTGGGCTCTATCTGTAAACAAAGCTGGAGAGTGGTTGGAACCACCTCCAAATGTACTGCCCCAGGGTTCAGTTTCTCTAAATGGGGCTGTTGAAACACAATTGGTATCTAGTTCTGTTGATTCTACTTATGAGGTTCAATCTTTAGAAATAGAAATGCAAGAAGAAAGTTTCAAAGGAATGCCATTTGTCCTTTGTTCTTCTGGAAACTGGATTAAGAACCAAGGCTCAGATTTCTACGCTGACTTTGGTGTTGCGGTCAAGAAAGTTCAGAAGGATGCTGGTGATGGGAAAGGTACTGCAAAGTATTTATTGGATAAAATAGCAGATCTGGAGAGTGAGGCGCAAAAATCCTTTATGCATCGGTTTAACATTGCAGCAGACTTGATAAATCAAGCAAAAGATGCTGGCGAATTAGGTCTTGCTGGTATTCTGGTGTGGATGAGGTTTATGGCTACGAGGCAGCTGATTTGGAATAAAAATTACAATGTCAAACCACGTGAGATTAGTAAAGCACAGGACAGGCTCACAGGCTTGCTCCAGAATATTTACACAAGTCATCCACAGTATCGAGAGCTTCTACGGATGATATTGTCTACTGTTGGACGTGGAGGAGAAGGGGATGTGGGACAGCGAATTCGAGATGAGATCCTTGTTATCCAGAGAAACTGTGATTGTAAGGGGGGAATGATGGAGGAATGGCATCAAAAGTTGCATAATAACACTAGCCCAGATGATGTTGTTATATGTCAGGCACTAATTGATTACATCAAAAGTGACTTTGATATTGGTGTTTACTGGAAAACTTTGAACAAGAATGGAATTACAAAAGAACGTCTTCTAAGCTATGACCGTGCAATCCACAATGAACCAAGTTTCAGGGCAGATCAGAAAGAGCGTCTTCTGCATGATCTGGGGAACTACATGAGAACTTTAAAGGCAGTTCACTCAGGTGCAGATCTTGAGTCTGCCATCACAAATTGTTTGGGTTACAGTGCTCAGGGTCAAGGCTTCATGGTTGGAGTACAAATAAATCCTGTATCTGGCCTGCCCTCTGAAGTTCCAGGACTGCTGCAATTTGTAATGGAACATGTTGAGGACAAGAATGTGGAAGTCCTTATTGAGGGTTTGCTTGAGGCTCGTCAGGAGCTGTGGCCGTTGCTCTCTAAGCCTAATGATCGCCTGAAGGATCTCTTATTTTTAGACATTGCCCTTGATTCTACTGTGAGAACCGCCATTGAGAGGGGATATGAAGAACTGAATAATGCTGGGCCGGAGAAAATTATGTACTTCATCTCAATGGTTCTAGAAAACCTGGCATTATCATCAGATGATAATGAGGATCTCATCTACTGTTTGAAGGGATGGAATCATGCGTTAAACATGTTAAAGAGTAATACCGATAACTGGGCATTATATGCAAAATCAATCCTAGACAGAACCCGCCTGGCGCTTGCAAACAAGGCAGAGTCGTATCACAGTATTTTACAGCCATCAGCAGAATACCTTGGATCAAAGCTTGGAGTGGACCAATGGGCTTTGAGCATATTTACTGAAGAAGTTATCCGTGCTGGATCAGCTGCCTCTTTGTCCGCACTCGTTAATCGACTTGATCCAGTTCTTCGGAAGACTGCTAATTTGGGAAGTTGGCAGGTTATCAGCCCAGTTGAAGTGGTTGGATATGTTGTAGTTGTGGACGAGTTGCTCACTGTCCAGAATAAAGTATATGAGAAGCCGACTATTTTGGTGGCAAGAAGTGTGAGAGGTGAGGAAGAAATTCCAGATGGCACAGTTGCTGTGCTAACACCTGATATGCCAGACGTCCTATCTCATGTTTCTGTCCGAGCAAGAAATAGCAAGGTTTGTTTTGCGACATGTTTTGACTCCAATATTCTAGCTGACCTCCAAGCTTGTAAAGGGAAGTTATTGCGTGTAAAGCCAACTCCTGCTGATGTAGTTTATAGCGAGGTTAATGAGAATGAGCTAGGAGATGCAAGTTCAACTAATTCAAATGAAGACATCCCAGCTCTGACATTAGTGAAGAAACAGTTTACGGGTAGATATGCCATATCATCTGATGAGTTCGCAAGTGAAATGGTTGGAGCTAAATCACGTAATATTTCTTATATAAAAGGAAAACTGCCCTCTTGGGTTGGAATTCCTACATCAGTTGCCCTACCATTTGGAGTATTTGAAAAAGTTCTTTCTGAAGATTCAAATAAGGCTGTTGCTGACAAGTTAGAAATTCTAAAGAAAAAGTTAAAAGAAGGAGATTTTGGTTCACTTGGGGAGATTCGAGAGACAGTTTTACAGTTGACAGCACCACCCCCTTTGGTGCAAGAGCTGAAGACTAAGATGCAAAGTTCAGGAATGCCTTGGCCTGGTGATGAGGGTGAACAACGATGGGAACAAGCATGGTTGGCTATAAAAAAGGTCTGGGCTTCAAAGTGGAATGAGAGAGCATACTTCAGCACAAGAAAAGTGAAATTAGACCATGATTATCTTTGCATGGCTGTCCTTGTTCAAGAAATAATAAATGCCGACTATGCATTCGTTATTCACACAACTAACCCATCTTCAGGAGACTCGTCGGAGATATATGCTGAGGTTGTCAAAGGTCTTGGAGAAACCTTAGTTGGAGCTTATCCTGGACGTGCTTTAAGTTTTATTAGCAAGAAAAACGCTCTGGACTCTCCTCAGGTGTTAGGTTACCCTAGCAAACCGATTGGCCTCTTTATCAGACAGTCCATAATCTTCCGATCCGATTCCAATGGTGAAGATCTTGAAGGTTATGCTGGTGCAGGCCTTTATGACAGTGTGCCTATGGATGCGGAAGAGGAAGTCGTGCTTGACTACTCATCTGACCCACTCATTACTGATGGGAACTTCCAAAAGACAATTCTGTCCAGCATTGCTCGCGCAGGAAGTGCAATCGAGGAGTTATATGGTTCAGCACAAGACATCGAAGGTGTGATCAGAGATGGGAAACTCTATGTTGTCCAGACAAGACCCCAGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009628 GO:0009631 GO:0009941 GO:0009987 GO:0016052 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016781 GO:0031967 GO:0031975 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050521 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPDK_N

Protein Analysis

1090

Amino Acids

120.83

Weight (kDa)

5.38

Isoelectric Point (pI)

37.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ig_N_CWD1	PF23166	48 - 166	2e-26	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
GWD1_pHisD	PF22973	633 - 745	3.3e-53	Alpha-glucan water dikinase, phosphohistidine-like domain
PPDK_N	PF01326	888 - 964	6.8e-13	Pyruvate phosphate dikinase, AMP/ATP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10760 AT1G10760 AT1G10760
fragaria_vesca	FvH4_4g25800 FvH4_4g25800 FvH4_4g25800 FvH4_4g25800
malus_domestica	MD13G1096800.v1.1 MD16G1097800.v1.1
prunus_persica	Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1
pyrus_communis	pycom13g08450 pycom16g08330
rosa_chinensis	RchiOBHm_Chr4g0433251
rosa_laevigata	RLG00000006771
rosa_multiflora	Rmu_co8055492.1_g000001 Rmu_sc0010643.1_g000001
rosa_roxburghii	Rroxscaffold_5G00374810
rosa_rugosa	Rorug04G0267200 Rorug04G0267300 Rorug04G0267400 Rorug04G0267400
rosa_samantha	Rh4AG321900 Rh4BG329300 Rh4CG344600
rosa_wichuraiana	Rw4G027860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	3154
AatII	GACGTC	1 cut(s)	2076
Acc16I	TGCGCA	1 cut(s)	54
Acc36I	ACCTGC	2 cut(s)	183, 1894
AccI	GTMKAC	1 cut(s)	848
AccII	CGCG	1 cut(s)	3171
AciI	CCGC	5 cut(s)	509, 1485, 1696, 1845, 3083
AclWI	GGATC	7 cut(s)	895, 1449, 1602, 1771, 1834, 1859, 3006
AcoI	YGGCCR	1 cut(s)	1406
AcsI	RAATTY	4 cut(s)	888, 2028, 2409, 2496
AcuI	CTGAAG	7 cut(s)	1329, 1457, 1827, 2481, 2618, 2704, 2814
AcvI	CACGTG	1 cut(s)	752
AcyI	GRCGYC	1 cut(s)	2073
AfaI	GTAC	5 cut(s)	183, 309, 550, 1278, 1541
AfiI	CCNNNNNNNGG	6 cut(s)	1295, 1375, 1494, 1659, 2633, 2831
AflIII	ACRYGT	3 cut(s)	1342, 1635, 2126
AjiI	CACGTC	2 cut(s)	860, 2906
AjnI	CCWGG	7 cut(s)	104, 315, 1315, 1566, 1698, 2632, 2899
AjuI	GAANNNNNNNTTGG	6 cut(s)	2511, 2543, 2871, 2903, 3135, 3167
AleI	CACNNNNGTG	1 cut(s)	1203
Alw21I	GWGCWC	1 cut(s)	1252
Alw26I	GTCTC	2 cut(s)	2546, 2829
AlwI	GGATC	7 cut(s)	895, 1449, 1602, 1771, 1834, 1859, 3006
AlwNI	CAGNNNCTG	2 cut(s)	1402, 2048
AoxI	GGCC	6 cut(s)	1297, 1406, 1523, 2630, 2980, 3055
ApeKI	GCWGC	4 cut(s)	629, 715, 1324, 1832
ApoI	RAATTY	4 cut(s)	888, 2028, 2409, 2496
ArsI	GACNNNNNNTTYG	6 cut(s)	873, 905, 1873, 1905, 2786, 2818
Asp700I	GAANNNNTTC	3 cut(s)	1089, 2028, 2451
AspLEI	GCGC	4 cut(s)	55, 598, 1705, 3173
AspS9I	GGNCC	3 cut(s)	172, 1523, 1780
AsuHPI	GGTGA	6 cut(s)	176, 548, 2030, 2648, 2657, 3038
AvaII	GGWCC	2 cut(s)	172, 1780
AxyI	CCTNAGG	1 cut(s)	2949
BanII	GRGCYC	1 cut(s)	262
BbrPI	CACGTG	1 cut(s)	752
BbsI	GAAGAC	5 cut(s)	1082, 1145, 1886, 2274, 2606
Bbv12I	GWGCWC	1 cut(s)	1252
BbvCI	CCTCAGC	1 cut(s)	2856
BbvI	GCAGC	4 cut(s)	641, 727, 1311, 1819
BceAI	ACGGC	1 cut(s)	1393
BciT130I	CCWGG	7 cut(s)	106, 317, 1317, 1568, 1700, 2634, 2901
BclI	TGATCA	1 cut(s)	3225
BcoDI	GTCTC	2 cut(s)	2546, 2829
BfaI	CTAG	7 cut(s)	363, 978, 1559, 1685, 2147, 2237, 2967
BfmI	CTRYAG	2 cut(s)	33, 154
BfoI	RGCGCY	1 cut(s)	1706
BfuAI	ACCTGC	2 cut(s)	183, 1894
BglII	AGATCT	3 cut(s)	580, 1210, 3031
BisI	GCNGC	4 cut(s)	630, 716, 1325, 1833
BlsI	GCNGC	4 cut(s)	631, 717, 1326, 1834
Bme1390I	CCNGG	7 cut(s)	106, 317, 1317, 1568, 1700, 2634, 2901
Bme18I	GGWCC	2 cut(s)	172, 1780
BmgBI	CACGTC	2 cut(s)	860, 2906
BmgT120I	GGNCC	3 cut(s)	172, 1523, 1780
BmiI	GGNNCC	2 cut(s)	133, 294
BmrFI	CCNGG	7 cut(s)	106, 317, 1317, 1568, 1700, 2634, 2901
BmrI	ACTGGG	2 cut(s)	1669, 1910
BmsI	GCATC	7 cut(s)	520, 622, 646, 964, 2233, 2598, 3070
BmuI	ACTGGG	2 cut(s)	1669, 1910
BpiI	GAAGAC	5 cut(s)	1082, 1145, 1886, 2274, 2606
BpmI	CTGGAG	3 cut(s)	300, 605, 776
Bpu10I	CCTNAGC	2 cut(s)	1251, 2856
BpuEI	CTTGAG	2 cut(s)	1235, 1406
BsaAI	YACGTR	2 cut(s)	752, 2366
BsaHI	GRCGYC	1 cut(s)	2073
BsaJI	CCNNGG	6 cut(s)	105, 315, 316, 475, 1759, 2627
Bsc4I	CCNNNNNNNGG	6 cut(s)	1295, 1375, 1494, 1659, 2633, 2831
Bse1I	ACTGG	5 cut(s)	467, 1055, 1664, 1868, 1916
Bse21I	CCTNAGG	1 cut(s)	2949
Bse3DI	GCAATG	3 cut(s)	624, 1458, 3162
BseBI	CCWGG	7 cut(s)	106, 317, 1317, 1568, 1700, 2634, 2901
BseDI	CCNNGG	6 cut(s)	105, 315, 316, 475, 1759, 2627
BseGI	GGATG	8 cut(s)	535, 699, 811, 843, 880, 1622, 2271, 3085
BseLI	CCNNNNNNNGG	6 cut(s)	1295, 1375, 1494, 1659, 2633, 2831
BseMI	GCAATG	3 cut(s)	624, 1458, 3162
BseMII	CTCAG	5 cut(s)	495, 1215, 1265, 2847, 2963
BseNI	ACTGG	5 cut(s)	467, 1055, 1664, 1868, 1916
BseRI	GAGGAG	3 cut(s)	879, 2937, 3203
BseXI	GCAGC	4 cut(s)	641, 727, 1311, 1819
BseYI	CCCAGC	2 cut(s)	1520, 2275
BsgI	GTGCAG	2 cut(s)	1227, 3072
Bsh1236I	CGCG	1 cut(s)	3171
BshFI	GGCC	6 cut(s)	1299, 1408, 1525, 2632, 2982, 3057
BsiHKAI	GWGCWC	1 cut(s)	1252
BsiSI	CCGG	1 cut(s)	1526
BslFI	GGGAC	1 cut(s)	894
BslI	CCNNNNNNNGG	6 cut(s)	1295, 1375, 1494, 1659, 2633, 2831
BsmAI	GTCTC	2 cut(s)	2546, 2829
BsmFI	GGGAC	1 cut(s)	894
BsmI	GAATGC	3 cut(s)	438, 2628, 2801
BsnI	GGCC	6 cut(s)	1299, 1408, 1525, 2632, 2982, 3057
Bsp1286I	GDGCHC	2 cut(s)	262, 1252
BspACI	CCGC	5 cut(s)	509, 1485, 1696, 1845, 3083
BspANI	GGCC	6 cut(s)	1299, 1408, 1525, 2632, 2982, 3057
BspCNI	CTCAG	5 cut(s)	494, 1214, 1264, 2848, 2962
BspFNI	CGCG	1 cut(s)	3171
BspLI	GGNNCC	2 cut(s)	133, 294
BspMI	ACCTGC	2 cut(s)	183, 1894
BspPI	GGATC	7 cut(s)	895, 1449, 1602, 1771, 1834, 1859, 3006
BsrDI	GCAATG	3 cut(s)	624, 1458, 3162
BsrI	ACTGG	5 cut(s)	467, 1055, 1664, 1868, 1916
BssECI	CCNNGG	6 cut(s)	105, 315, 316, 475, 1759, 2627
BssNI	GRCGYC	1 cut(s)	2073
BssT1I	CCWWGG	3 cut(s)	475, 1759, 2627
Bst2UI	CCWGG	7 cut(s)	106, 317, 1317, 1568, 1700, 2634, 2901
Bst6I	CTCTTC	1 cut(s)	3081
BstACI	GRCGYC	1 cut(s)	2073
BstAPI	GCANNNNNTGC	3 cut(s)	656, 1670, 3179
BstBAI	YACGTR	2 cut(s)	752, 2366
BstC8I	GCNNGC	6 cut(s)	661, 788, 1301, 1707, 3055, 3169
BstDEI	CTNAG	9 cut(s)	481, 1094, 1201, 1251, 1419, 2604, 2856, 2884, 2949
BstEII	GGTNACC	1 cut(s)	2960
BstENI	CCTNNNNNAGG	1 cut(s)	1373
BstF5I	GGATG	8 cut(s)	535, 699, 811, 843, 880, 1622, 2271, 3085
BstFNI	CGCG	1 cut(s)	3171
BstH2I	RGCGCY	1 cut(s)	1706
BstHHI	GCGC	4 cut(s)	55, 598, 1705, 3173
BstMAI	GTCTC	2 cut(s)	2546, 2829
BstNI	CCWGG	7 cut(s)	106, 317, 1317, 1568, 1700, 2634, 2901
BstNSI	RCATGY	4 cut(s)	23, 1346, 1639, 2130
BstPI	GGTNACC	1 cut(s)	2960
BstSCI	CCNGG	7 cut(s)	104, 315, 1315, 1566, 1698, 2632, 2899
BstSFI	CTRYAG	2 cut(s)	33, 154
BstUI	CGCG	1 cut(s)	3171
BstV1I	GCAGC	4 cut(s)	641, 727, 1311, 1819
BstV2I	GAAGAC	5 cut(s)	1082, 1145, 1886, 2274, 2606
BstX2I	RGATCY	6 cut(s)	580, 900, 1210, 1441, 1594, 3031
BstYI	RGATCY	6 cut(s)	580, 900, 1210, 1441, 1594, 3031
Bsu36I	CCTNAGG	1 cut(s)	2949
BsuRI	GGCC	6 cut(s)	1299, 1408, 1525, 2632, 2982, 3057
BtrI	CACGTC	2 cut(s)	860, 2906
BtsCI	GGATG	8 cut(s)	535, 699, 811, 843, 880, 1622, 2271, 3085
BtsIMutI	CAGTG	3 cut(s)	1252, 1959, 3073
BveI	ACCTGC	2 cut(s)	183, 1894
Cac8I	GCNNGC	6 cut(s)	661, 788, 1301, 1707, 3055, 3169
CaiI	CAGNNNCTG	2 cut(s)	1402, 2048
CfoI	GCGC	4 cut(s)	55, 598, 1705, 3173
Cfr13I	GGNCC	3 cut(s)	172, 1523, 1780
CseI	GACGC	1 cut(s)	1139
Csp6I	GTAC	5 cut(s)	182, 308, 549, 1277, 1540
CspCI	CAANNNNNGTGG	2 cut(s)	2565, 2600
CviQI	GTAC	5 cut(s)	182, 308, 549, 1277, 1540
DdeI	CTNAG	9 cut(s)	481, 1094, 1201, 1251, 1419, 2604, 2856, 2884, 2949
DraI	TTTAAA	1 cut(s)	1188
DrdI	GACNNNNNNGTC	1 cut(s)	3154
DseDI	GACNNNNNNGTC	1 cut(s)	3154
EaeI	YGGCCR	1 cut(s)	1406
Eam1104I	CTCTTC	1 cut(s)	3081
EarI	CTCTTC	1 cut(s)	3081
Eco130I	CCWWGG	3 cut(s)	475, 1759, 2627
Eco147I	AGGCCT	1 cut(s)	3057
Eco24I	GRGCYC	1 cut(s)	262
Eco32I	GATATC	1 cut(s)	232
Eco47I	GGWCC	2 cut(s)	172, 1780
Eco57I	CTGAAG	7 cut(s)	1329, 1457, 1827, 2481, 2618, 2704, 2814
Eco72I	CACGTG	1 cut(s)	752
Eco81I	CCTNAGG	1 cut(s)	2949
Eco91I	GGTNACC	1 cut(s)	2960
EcoNI	CCTNNNNNAGG	1 cut(s)	1373
EcoO109I	RGGNCCY	1 cut(s)	172
EcoO65I	GGTNACC	1 cut(s)	2960
EcoRI	GAATTC	2 cut(s)	888, 2409
EcoRII	CCWGG	7 cut(s)	104, 315, 1315, 1566, 1698, 2632, 2899
EcoRV	GATATC	1 cut(s)	232
EcoT14I	CCWWGG	3 cut(s)	475, 1759, 2627
EcoT22I	ATGCAT	2 cut(s)	615, 2803
EcoT38I	GRGCYC	1 cut(s)	262
ErhI	CCWWGG	3 cut(s)	475, 1759, 2627
FaqI	GGGAC	1 cut(s)	894
FauI	CCCGC	1 cut(s)	1703
FbaI	TGATCA	1 cut(s)	3225
FblI	GTMKAC	1 cut(s)	848
Fnu4HI	GCNGC	4 cut(s)	630, 716, 1325, 1833
FokI	GGATG	8 cut(s)	542, 706, 798, 850, 887, 1629, 2258, 3092
FriOI	GRGCYC	1 cut(s)	262
Fsp4HI	GCNGC	4 cut(s)	630, 716, 1325, 1833
FspBI	CTAG	7 cut(s)	363, 978, 1559, 1685, 2147, 2237, 2967
FspI	TGCGCA	1 cut(s)	54
GlaI	GCGC	4 cut(s)	54, 597, 1704, 3172
GluI	GCNGC	4 cut(s)	630, 716, 1325, 1833
GsaI	CCCAGC	2 cut(s)	1524, 2279
GsuI	CTGGAG	3 cut(s)	300, 605, 776
HaeII	RGCGCY	1 cut(s)	1706
HaeIII	GGCC	6 cut(s)	1299, 1408, 1525, 2632, 2982, 3057
HapII	CCGG	1 cut(s)	1526
HgaI	GACGC	1 cut(s)	1139
HhaI	GCGC	4 cut(s)	55, 598, 1705, 3173
Hin1I	GRCGYC	1 cut(s)	2073
Hin6I	GCGC	4 cut(s)	53, 596, 1703, 3171
HinP1I	GCGC	4 cut(s)	53, 596, 1703, 3171
HincII	GTYRAC	1 cut(s)	2568
HindII	GTYRAC	1 cut(s)	2568
HindIII	AAGCTT	2 cut(s)	1769, 2160
HpaII	CCGG	1 cut(s)	1526
HphI	GGTGA	6 cut(s)	176, 548, 2030, 2648, 2657, 3038
Hpy99I	CGWCG	1 cut(s)	2845
HpyAV	CCTTC	7 cut(s)	520, 863, 1432, 1606, 2513, 3032, 3212
HpyCH4IV	ACGT	6 cut(s)	751, 859, 1087, 2073, 2365, 2905
HpyF3I	CTNAG	9 cut(s)	481, 1094, 1201, 1251, 1419, 2604, 2856, 2884, 2949
HpySE526I	ACGT	6 cut(s)	751, 859, 1087, 2073, 2365, 2905
Hsp92I	GRCGYC	1 cut(s)	2073
HspAI	GCGC	4 cut(s)	53, 596, 1703, 3171
Ksp22I	TGATCA	1 cut(s)	3225
LmnI	GCTCC	4 cut(s)	795, 1399, 2354, 2891
Lsp1109I	GCAGC	4 cut(s)	641, 727, 1311, 1819
LweI	GCATC	7 cut(s)	520, 622, 646, 964, 2233, 2598, 3070
MaeI	CTAG	7 cut(s)	363, 978, 1559, 1685, 2147, 2237, 2967
MaeII	ACGT	6 cut(s)	751, 859, 1087, 2073, 2365, 2905
MaeIII	GTNAC	4 cut(s)	187, 1028, 1241, 2960
MfeI	CAATTG	1 cut(s)	353
MflI	RGATCY	6 cut(s)	580, 900, 1210, 1441, 1594, 3031
MhlI	GDGCHC	2 cut(s)	262, 1252
MlyI	GAGTC	5 cut(s)	1226, 1730, 2129, 2831, 2936
MmeI	TCCRAC	8 cut(s)	270, 835, 1252, 1909, 2332, 2386, 2823, 2869
Mph1103I	ATGCAT	2 cut(s)	615, 2803
MroXI	GAANNNNTTC	3 cut(s)	1089, 2028, 2451
MslI	CAYNNNNRTG	2 cut(s)	1203, 1550
MspA1I	CMGCKG	2 cut(s)	718, 1832
MspI	CCGG	1 cut(s)	1526
MspR9I	CCNGG	7 cut(s)	106, 317, 1317, 1568, 1700, 2634, 2901
MunI	CAATTG	1 cut(s)	353
Mva1269I	GAATGC	3 cut(s)	438, 2628, 2801
MvaI	CCWGG	7 cut(s)	106, 317, 1317, 1568, 1700, 2634, 2901
MvnI	CGCG	1 cut(s)	3171
NlaIV	GGNNCC	2 cut(s)	133, 294
NmuCI	GTSAC	2 cut(s)	187, 1028
NsbI	TGCGCA	1 cut(s)	54
NsiI	ATGCAT	2 cut(s)	615, 2803
NspI	RCATGY	4 cut(s)	23, 1346, 1639, 2130
OliI	CACNNNNGTG	1 cut(s)	1203
PasI	CCCWGGG	1 cut(s)	316
PceI	AGGCCT	1 cut(s)	3057
PciI	ACATGT	3 cut(s)	1342, 1635, 2126
PctI	GAATGC	3 cut(s)	438, 2628, 2801
PdmI	GAANNNNTTC	3 cut(s)	1089, 2028, 2451
PfeI	GAWTC	8 cut(s)	7, 62, 374, 1469, 1621, 2465, 2545, 3017
PfoI	TCCNGGA	2 cut(s)	1315, 2899
PkrI	GCNGC	4 cut(s)	631, 717, 1326, 1834
PleI	GAGTC	5 cut(s)	1225, 1729, 2129, 2831, 2936
PmaCI	CACGTG	1 cut(s)	752
PmlI	CACGTG	1 cut(s)	752
PpsI	GAGTC	5 cut(s)	1225, 1729, 2129, 2831, 2936
Ppu21I	YACGTR	2 cut(s)	752, 2366
PpuMI	RGGWCCY	1 cut(s)	172
PscI	ACATGT	3 cut(s)	1342, 1635, 2126
Psp5II	RGGWCCY	1 cut(s)	172
Psp6I	CCWGG	7 cut(s)	104, 315, 1315, 1566, 1698, 2632, 2899
PspCI	CACGTG	1 cut(s)	752
PspEI	GGTNACC	1 cut(s)	2960
PspFI	CCCAGC	2 cut(s)	1520, 2275
PspGI	CCWGG	7 cut(s)	104, 315, 1315, 1566, 1698, 2632, 2899
PspN4I	GGNNCC	2 cut(s)	133, 294
PspPI	GGNCC	3 cut(s)	172, 1523, 1780
PspPPI	RGGWCCY	1 cut(s)	172
PstNI	CAGNNNCTG	2 cut(s)	1402, 2048
PsuI	RGATCY	6 cut(s)	580, 900, 1210, 1441, 1594, 3031
PvuII	CAGCTG	2 cut(s)	718, 1832
RsaI	GTAC	5 cut(s)	183, 309, 550, 1278, 1541
RsaNI	GTAC	5 cut(s)	182, 308, 549, 1277, 1540
RseI	CAYNNNNRTG	2 cut(s)	1203, 1550
SatI	GCNGC	4 cut(s)	630, 716, 1325, 1833
Sau96I	GGNCC	3 cut(s)	172, 1523, 1780
SchI	GAGTC	5 cut(s)	1226, 1730, 2129, 2831, 2936
ScrFI	CCNGG	7 cut(s)	106, 317, 1317, 1568, 1700, 2634, 2901
SduI	GDGCHC	2 cut(s)	262, 1252
SfaNI	GCATC	7 cut(s)	520, 622, 646, 964, 2233, 2598, 3070
SfcI	CTRYAG	2 cut(s)	33, 154
SinI	GGWCC	2 cut(s)	172, 1780
SmiMI	CAYNNNNRTG	2 cut(s)	1203, 1550
SmlI	CTYRAG	2 cut(s)	1214, 1385
SmoI	CTYRAG	2 cut(s)	1214, 1385
SseBI	AGGCCT	1 cut(s)	3057
SsiI	CCGC	5 cut(s)	509, 1485, 1696, 1845, 3083
SspI	AATATT	3 cut(s)	799, 2143, 2371
SspMI	CTAG	7 cut(s)	363, 978, 1559, 1685, 2147, 2237, 2967
StuI	AGGCCT	1 cut(s)	3057
StyD4I	CCNGG	7 cut(s)	104, 315, 1315, 1566, 1698, 2632, 2899
StyI	CCWWGG	3 cut(s)	475, 1759, 2627
TaiI	ACGT	6 cut(s)	754, 862, 1090, 2076, 2368, 2908
TaqI	TCGA	6 cut(s)	823, 892, 1858, 2548, 3186, 3216
TatI	WGTACW	3 cut(s)	307, 1276, 1539
TfiI	GAWTC	8 cut(s)	7, 62, 374, 1469, 1621, 2465, 2545, 3017
TscAI	CASTG	3 cut(s)	1252, 1966, 3073
TseFI	GTSAC	2 cut(s)	187, 1028
TseI	GCWGC	4 cut(s)	629, 715, 1324, 1832
Tsp45I	GTSAC	2 cut(s)	187, 1028
TspGWI	ACGGA	3 cut(s)	124, 850, 1808
TspRI	CASTG	3 cut(s)	1252, 1966, 3073
VpaK11BI	GGWCC	2 cut(s)	172, 1780
XagI	CCTNNNNNAGG	1 cut(s)	1373
XapI	RAATTY	4 cut(s)	888, 2028, 2409, 2496
XbaI	TCTAGA	1 cut(s)	1558
XceI	RCATGY	4 cut(s)	23, 1346, 1639, 2130
XmiI	GTMKAC	1 cut(s)	848
XmnI	GAANNNNTTC	3 cut(s)	1089, 2028, 2451
XspI	CTAG	7 cut(s)	363, 978, 1559, 1685, 2147, 2237, 2967
ZraI	GACGTC	1 cut(s)	2074
Zsp2I	ATGCAT	2 cut(s)	615, 2803

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.