Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0005249

Physical Location & Seq

Genomic Coordinates Forward (+)

60405444 .. 60417957

Gene Structure 12514 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG344600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4224 bp

ATGAGCAACTCGGTGAGCCATAATATTCTCCAGCAGAATCTGCTTCAGAGCAAAATCAATTCTTCAGGCATTCCTGCCAACACATTGTTACAACCCAAGTGTATACATCCAGTGCCAGCGCAGCCGCGCAAGTCTTCTTCTATATCCAAGAAATTTTGTGGCAACAGTTTGCAGAAGCCGAAGCTGGCCATGGGGTCTCGTCCACCTGTTACATTTGTTCCTTGCGCTGTACTAACCACGGATCCGCATTCTGATCAGCTTGCCGGGAAATACAACCTTGATGAGAACATTGAATTGCAGGTTTATGTTGATGCTTCCTCTCCAGGGTCTGCCACTAGAGTAGATATTCAGGTCTCATATGGCGGTGACTCTTTGGTTCTTCATTGGGGTGGGATAAAAGATAGAAAAGAAAAATGGGTGCTTCCTTCTCGTCACCCAGATGGAACCAAAGAATATAAGAAGAGAGCTCTTAGAACTCCTTTTGCGAAATCTGGCTCCAATTCCGTACTTAAAATTGAGATTGATGATCCTGCAATACAAGCTATAGAGTTTCTCATAGTTGATGAAAGTCAGAACAGATGGTTTAAGAACAACGGCGGCAACTTTCATGTTAAATTACCTTTGAAAGAGAAGTTGATATCAAATGTTTCAGTTCCTGAAGATTTAGTGCAAATTCAAGCATATCTGAGGTGGGAGCGAAAGGGTAAACAGATGTACACACCAGAACAAGAGAAGGTGGAGTATGAAGCAGCCCGAACTGAGCTACTGGAGGAAGTAGCCAGGGGTGCTTCCATACAAGAGCTCCAAGCAAGGCTGACTAAGAAAACTGATTCTGGTAACAGTCAGGAACCATCTCATGCTGGTAACAAAGAAGTTTCCAAGGCCGCAAAGAAACATGAGAGTAAACAGGTTTTCAGAACTGGAGGAATTAAGCGGAAGCAACGAGACTTAATGAAGATTCTCAACAAACATGCTACTAAACCTGTAGATGAAGCTAAACTTGCACATGAAGAATCAACGAAACCAAAAGCCTTGAAAGCAGTTGAGCTATTTGCCAAGGCAAAAGAAGAACAAGATGGTGCTTCCGTCCTGAACAAAAAAATCTACAGGCTCGGTGATAAGGACCTGCTGGTACTTGTGACAAAGGTTGCTGACAAGATAAAGGTTCATCTGGCGACAGATATCAAAGAGCCAATTAACCTTCATTGGGCTCTATCTGTAAACAAAGCTGGAGAGTGGTTGGAACCACCTCCAAATGTACTGCCCCAGGGTTCAGTTTCTCTAAATGGGGCTGTTGAAACACAATTTGTATCTAGTTCTGTTGATTCTAATTATGAGGTTCAATCTTTAGAAATAGAAATGCAAGAGGAAAGTTTCAAAGGAATGCCATTTGTCCTTTGTTCTTCTGGAAACTGGATTAAGAACCAAGGCTCAGATTTCTACGCTGACTTTGGTGTTGCGGTCAAGAAAGTTCAGAAGGATGCTGGTGACGGGAAAGGTACTGCAAAGGATTTATTGGATAAAATAGCAGATCTGGAGAGTGAGGCGCAAAAATCCTTTATGCATCGGTTTAACATTGCAGCAGACTTGATAAATCAAGCAAAAGATGCTGGCGAATTAGGTCTTGCAGGTATTCTGGTGTGGATGAGGTTTATGGCTACGAGGCAGCTGATTTGGAATAAAAATTACAATGTCAAACCACGTGAGATTAGTAAAGCACAGGACAGGCTCACAGGCTTGCTCCAGAGTATTTACACAAGTCATCCACAGTATCGAGAACTTTTACGGATGATATTGTCTACTGTTGGACGTGGAGGAGAAGGGGATGTGGGACAGCGAATTCGAGATGAGATCCTTGTTATTCAGAGAAACTGTGAGTGTAAGGGCGGAATGATGGAGGAATGGCATCAAAAGTTGCATAATAACACTAGCCCAGATGATGTTGTTATATGTCAGGCGCTAATTGATTACATCAAAAGTGACTTTGATATTGGTGTTTACTGGAAAACTTTGAACAACAATGGAATTACAAAAGAACGTCTTCTAAGCTATGACCGTGCAATCCACAATGAACCAAGTTTCAGGAGAGATCAGAAAGAGCGTCTTCTGCATGATCTGGGGAACTACATGAGAACTTTAAAGGCAGTTCACTCAGGTGCAGATCTTGAGTCTGCCATCACAAATTGTTTGGGTTACAGTGCTCAGGGTCAAGGCTTCATGGTTGGAGTACAAATAAATCCTGTATCTGGCCTGCCCTCTGAAGTTCCAGGACTGCTGCAATTTGTAATGGAACATGTTGAGGACAAGAATGTGGAAGTCCTTATTGAGGGTTTGCTTGAGGCTCGTCAGGAGCTGTGGTCATTGCTCTCTAAGCCTAATGATCGCCTGAAGGATCTCTTATTTTTGGACATTGCCCTTGATTCTACTGTGAGAACCGCCATTGAGAGGGGATATGAAGAACTGAATAATGCTGGGCCAGAGAAAATTATGTACTTCATCTCAATGGTTCTAGAAAACCTGGCATTATCGTCAGATGATAATGAGGATCTCATTTACTGTTTGAAGGGATGGAATCATGCATTAAACATGCTAAAGAGTAATACCGATAACTGGGCATTATATGCAAAATCAATCCTAGACAGAACCCGCCTGGCACTTGCAAACAAGGCAGAGTCGTATCACAGTATTTTACAGCCATCAGCAGAGTACCTTGGATCAAAGCTTGGAGTGGACCAAGGGGCTTTGAGCATATTTACTGAAGAAGTTATCCGTGCTGGATCAGCTGCCTCTTTGTCCGCACTCGTTAATCGACTTGATCCAGTTCTTCGGAAGACTGCTAATTTGGGAAGTTGGCAGGTTATCAGCCCAGTTGAAGTGGTTGGATATGTTGTAGTTGTGGACGAGTTGCTCACTGTCCAGAATAAAGTATACGATAAGCCGACAATTTTGGTGGCAAGAAGTGTAAGAGGTGAGGAAGAAATTCCAGATGGCACAGTTGCTGTGCTAACACCTGATATGCCAGACGTCCTATCTCATGTTTCTGTCCGAGCAAGAAATAGCAAGGTTTGTTTTGCGACGTGTTTTGACTCCAATATTCTAGCTGACCTCCAAGCTTGTGAAGGGAAGTTATTGCGTGTAAAGCCAACTCCTGCTGATGTAGTTTATAGCGAGGTTAATGAGAGTGAGCTAGGAGATGCAAGTTCAACTAATACAAATGAAGACATCCCAGCTCTGACATTAGTGAAGAAACAGTTTACGGGTAGATATGCCATATCATCTGATGAGTTCACAAGTGAAATGGTTGGAGCTAAATCACGTAATATTTCTTATATAAAAGGAAAACTGCCCTCTTGGGTTGGAATTCCTACATCAGTTGCCCTACCATTTGGAGTATTTGAAAAAGTTCTTTCTGAAGATTCAAATAAGGCTGTTGCTGACAAGTTAGAAATTCTAAAGAAAAAGTTAAAAGAAGGAGATTTTGGTTCACTTGGGGAGATTCGAGAGACAGTTTTACAGTTGACAGCACCACCCCCGTTGGTGCAAGAGCTGAAGACTAAGATGCAAAGTTCAGGAATGCCTTGGCCTGGTGATGAGGGTGAACAACGATGGGAACAAGCATGGTTGGCTATAAAAAAGGTCTGGGCTTCAAAGTGGAATGAGAGAGCATACTTCAGCACAAGAAAAGTGAAATTAGACCATGATTATCTTTGCATGGCTGTCCTTGTTCAAGAAATAATAAGTGCCGACTATGCATTCGTTATTCACACAACTAACCCATCTTCAGGAGACTCGTCGGAGATATATGCCGAGGTTGTCAAAGGTCTTGGAGAAACCTTAGTTGGAGCTTATCCTGGACGTGCCTTAAGTTTTATTAGCAAGAAAAATGCTCTGGACTCTCCTCAGGTGTTAGGTTACCCTAGCAAACCGGTTGGCCTCTTTATCAGACGGTCCATAATCTTCCGATCCGATTCCAATGGTGAAGATCTTGAAGGTTATGCTGGTGCAGGCCTTTATGACAGTGTGCCTATGGATGCGGAAGAGGAAGTCGTGCTTGACTACTCATCTGACCCACTCGTTACTGATGGGAACTTCCAAAAGACAATTCTGTCCAGCATTGCTCGCGCAGGAAGTGCAATCGAGGAGTTATATGGTTCAGCACAAGACATTGAAGGTGTGATCAGAGATGGGAAACTCTATGTTGTCCAGACAAGACCCCAGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009628 GO:0009631 GO:0009941 GO:0009987 GO:0016052 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016781 GO:0031967 GO:0031975 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050521 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPDK_N

Protein Analysis

1407

Amino Acids

156.04

Weight (kDa)

6.04

Isoelectric Point (pI)

41.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ig_N_CWD1	PF23166	89 - 206	6.5e-41	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
DUF7067	PF23229	220 - 275	4.1e-28	Alpha-glucan water dikinase, DUF7067
Ig_N_CWD1	PF23166	365 - 483	3e-27	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
GWD1_pHisD	PF22973	950 - 1062	2.7e-53	Alpha-glucan water dikinase, phosphohistidine-like domain
PPDK_N	PF01326	1205 - 1406	4.4e-19	Pyruvate phosphate dikinase, AMP/ATP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10760 AT1G10760 AT1G10760
fragaria_vesca	FvH4_4g25800 FvH4_4g25800 FvH4_4g25800 FvH4_4g25800
malus_domestica	MD13G1096800.v1.1 MD16G1097800.v1.1
prunus_persica	Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1
pyrus_communis	pycom13g08450 pycom16g08330
rosa_chinensis	RchiOBHm_Chr4g0433251
rosa_laevigata	RLG00000006771
rosa_multiflora	Rmu_co8055492.1_g000001 Rmu_sc0010643.1_g000001
rosa_roxburghii	Rroxscaffold_5G00374810
rosa_rugosa	Rorug04G0267200 Rorug04G0267300 Rorug04G0267400 Rorug04G0267400
rosa_samantha	Rh4AG321900 Rh4BG329300 Rh4CG344600
rosa_wichuraiana	Rw4G027860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	4105
AatII	GACGTC	1 cut(s)	3027
Acc36I	ACCTGC	4 cut(s)	289, 1134, 1619, 2845
AccI	GTMKAC	3 cut(s)	103, 1799, 2928
AccII	CGCG	2 cut(s)	127, 4122
AcoI	YGGCCR	1 cut(s)	186
AcsI	RAATTY	6 cut(s)	152, 672, 1839, 2979, 3360, 3447
AcvI	CACGTG	1 cut(s)	1703
AcyI	GRCGYC	1 cut(s)	3024
AfaI	GTAC	9 cut(s)	231, 507, 716, 1134, 1260, 1501, 2229, 2492, 2708
AfiI	CCNNNNNNNGG	8 cut(s)	324, 1207, 2246, 2326, 2445, 2610, 3584, 3782
AflII	CTTAAG	1 cut(s)	3862
AflIII	ACRYGT	2 cut(s)	2293, 3077
AgeI	ACCGGT	1 cut(s)	3925
AjiI	CACGTC	3 cut(s)	1811, 3078, 3857
AjnI	CCWGG	8 cut(s)	322, 779, 1266, 2266, 2517, 2649, 3583, 3850
AjuI	GAANNNNNNNTTGG	6 cut(s)	3462, 3494, 3822, 3854, 4086, 4118
AleI	CACNNNNGTG	1 cut(s)	2154
Alw21I	GWGCWC	3 cut(s)	469, 804, 2203
Alw26I	GTCTC	5 cut(s)	201, 358, 939, 3497, 3780
AlwNI	CAGNNNCTG	5 cut(s)	40, 329, 656, 2353, 2999
AoxI	GGCC	7 cut(s)	186, 882, 2248, 2474, 3581, 3931, 4006
ApeKI	GCWGC	6 cut(s)	121, 749, 1580, 1666, 2275, 2783
ApoI	RAATTY	6 cut(s)	152, 672, 1839, 2979, 3360, 3447
ArsI	GACNNNNNNTTYG	6 cut(s)	1824, 1856, 2824, 2856, 3737, 3769
AsiGI	ACCGGT	1 cut(s)	3925
Asp700I	GAANNNNTTC	3 cut(s)	2040, 2979, 3402
AspLEI	GCGC	6 cut(s)	121, 129, 227, 1549, 1960, 4124
AspS9I	GGNCC	4 cut(s)	1123, 2474, 2731, 3948
AsuC2I	CCSGG	1 cut(s)	265
AsuHPI	GGTGA	9 cut(s)	25, 377, 425, 1127, 1499, 2981, 3599, 3608, 3989
AvaII	GGWCC	3 cut(s)	1123, 2731, 3948
AxyI	CCTNAGG	1 cut(s)	3900
BalI	TGGCCA	1 cut(s)	188
BamHI	GGATCC	1 cut(s)	241
BanII	GRGCYC	3 cut(s)	469, 804, 1213
BbrPI	CACGTG	1 cut(s)	1703
BbsI	GAAGAC	6 cut(s)	126, 2033, 2096, 2837, 3225, 3557
Bbv12I	GWGCWC	3 cut(s)	469, 804, 2203
BbvI	GCAGC	6 cut(s)	133, 761, 1592, 1678, 2262, 2770
BceAI	ACGGC	1 cut(s)	610
BciT130I	CCWGG	8 cut(s)	324, 781, 1268, 2268, 2519, 2651, 3585, 3852
BclI	TGATCA	2 cut(s)	253, 4176
BcnI	CCSGG	1 cut(s)	265
BcoDI	GTCTC	5 cut(s)	201, 358, 939, 3497, 3780
BfaI	CTAG	8 cut(s)	336, 1314, 1929, 2510, 2636, 3098, 3188, 3918
BfmI	CTRYAG	3 cut(s)	543, 984, 1105
BfoI	RGCGCY	1 cut(s)	1961
BfrI	CTTAAG	1 cut(s)	3862
BfuAI	ACCTGC	4 cut(s)	289, 1134, 1619, 2845
BglII	AGATCT	3 cut(s)	1531, 2161, 3982
BisI	GCNGC	9 cut(s)	122, 125, 598, 750, 885, 1581, 1667, 2276, 2784
BlsI	GCNGC	9 cut(s)	123, 126, 599, 751, 886, 1582, 1668, 2277, 2785
Bme1390I	CCNGG	9 cut(s)	265, 324, 781, 1268, 2268, 2519, 2651, 3585, 3852
Bme18I	GGWCC	3 cut(s)	1123, 2731, 3948
BmgBI	CACGTC	3 cut(s)	1811, 3078, 3857
BmgT120I	GGNCC	4 cut(s)	1123, 2474, 2731, 3948
BmiI	GGNNCC	5 cut(s)	243, 445, 496, 849, 1245
BmrFI	CCNGG	9 cut(s)	265, 324, 781, 1268, 2268, 2519, 2651, 3585, 3852
BmrI	ACTGGG	2 cut(s)	2620, 2861
BmsI	GCATC	8 cut(s)	301, 1471, 1573, 1597, 1915, 3184, 3549, 4021
BmuI	ACTGGG	2 cut(s)	2620, 2861
BpiI	GAAGAC	6 cut(s)	126, 2033, 2096, 2837, 3225, 3557
BpmI	CTGGAG	7 cut(s)	14, 306, 788, 942, 1251, 1556, 1727
Bpu10I	CCTNAGC	1 cut(s)	2202
BpuEI	CTTGAG	2 cut(s)	2186, 2357
BpuMI	CCSGG	1 cut(s)	265
BsaAI	YACGTR	2 cut(s)	1703, 3317
BsaHI	GRCGYC	1 cut(s)	3024
BsaI	GGTCTC	2 cut(s)	201, 358
BsaWI	WCCGGW	1 cut(s)	3925
BsaXI	ACNNNNNCTCC	4 cut(s)	786, 816, 2342, 2372
Bsc4I	CCNNNNNNNGG	8 cut(s)	324, 1207, 2246, 2326, 2445, 2610, 3584, 3782
Bse118I	RCCGGY	1 cut(s)	3925
Bse1I	ACTGG	8 cut(s)	110, 771, 925, 1418, 2006, 2615, 2819, 2867
Bse21I	CCTNAGG	1 cut(s)	3900
Bse3DI	GCAATG	4 cut(s)	1575, 2360, 2409, 4113
BseBI	CCWGG	8 cut(s)	324, 781, 1268, 2268, 2519, 2651, 3585, 3852
BseGI	GGATG	9 cut(s)	106, 1486, 1650, 1762, 1794, 1831, 2573, 3222, 4036
BseLI	CCNNNNNNNGG	8 cut(s)	324, 1207, 2246, 2326, 2445, 2610, 3584, 3782
BseMI	GCAATG	4 cut(s)	1575, 2360, 2409, 4113
BseMII	CTCAG	6 cut(s)	677, 750, 1446, 2166, 2216, 3914
BseNI	ACTGG	8 cut(s)	110, 771, 925, 1418, 2006, 2615, 2819, 2867
BseRI	GAGGAG	3 cut(s)	1830, 3888, 4154
BseXI	GCAGC	6 cut(s)	133, 761, 1592, 1678, 2262, 2770
BseYI	CCCAGC	2 cut(s)	2471, 3226
BsgI	GTGCAG	2 cut(s)	2178, 4023
Bsh1236I	CGCG	2 cut(s)	127, 4122
BshFI	GGCC	7 cut(s)	188, 884, 2250, 2476, 3583, 3933, 4008
BshTI	ACCGGT	1 cut(s)	3925
BsiHKAI	GWGCWC	3 cut(s)	469, 804, 2203
BsiSI	CCGG	2 cut(s)	264, 3926
BslFI	GGGAC	1 cut(s)	1845
BslI	CCNNNNNNNGG	8 cut(s)	324, 1207, 2246, 2326, 2445, 2610, 3584, 3782
BsmAI	GTCTC	5 cut(s)	201, 358, 939, 3497, 3780
BsmFI	GGGAC	1 cut(s)	1845
BsmI	GAATGC	5 cut(s)	69, 247, 1389, 3579, 3752
BsnI	GGCC	7 cut(s)	188, 884, 2250, 2476, 3583, 3933, 4008
Bso31I	GGTCTC	2 cut(s)	201, 358
Bsp1286I	GDGCHC	4 cut(s)	469, 804, 1213, 2203
Bsp1407I	TGTACA	1 cut(s)	714
Bsp19I	CCATGG	1 cut(s)	189
BspANI	GGCC	7 cut(s)	188, 884, 2250, 2476, 3583, 3933, 4008
BspCNI	CTCAG	6 cut(s)	678, 751, 1445, 2165, 2215, 3913
BspFNI	CGCG	2 cut(s)	127, 4122
BspLI	GGNNCC	5 cut(s)	243, 445, 496, 849, 1245
BspMI	ACCTGC	4 cut(s)	289, 1134, 1619, 2845
BspTI	CTTAAG	1 cut(s)	3862
BspTNI	GGTCTC	2 cut(s)	201, 358
BsrDI	GCAATG	4 cut(s)	1575, 2360, 2409, 4113
BsrFI	RCCGGY	1 cut(s)	3925
BsrGI	TGTACA	1 cut(s)	714
BsrI	ACTGG	8 cut(s)	110, 771, 925, 1418, 2006, 2615, 2819, 2867
BssAI	RCCGGY	1 cut(s)	3925
BssNAI	GTATAC	2 cut(s)	104, 2929
BssNI	GRCGYC	1 cut(s)	3024
BssT1I	CCWWGG	7 cut(s)	189, 879, 1056, 1426, 2710, 2734, 3578
Bst1107I	GTATAC	2 cut(s)	104, 2929
Bst2UI	CCWGG	8 cut(s)	324, 781, 1268, 2268, 2519, 2651, 3585, 3852
Bst6I	CTCTTC	2 cut(s)	455, 4032
BstACI	GRCGYC	1 cut(s)	3024
BstAFI	CTTAAG	1 cut(s)	3862
BstAPI	GCANNNNNTGC	4 cut(s)	40, 1607, 2621, 4130
BstAUI	TGTACA	1 cut(s)	714
BstBAI	YACGTR	2 cut(s)	1703, 3317
BstC8I	GCNNGC	8 cut(s)	117, 186, 261, 1612, 1739, 2252, 4006, 4120
BstDSI	CCRYGG	2 cut(s)	189, 237
BstEII	GGTNACC	1 cut(s)	3911
BstENI	CCTNNNNNAGG	1 cut(s)	2324
BstF5I	GGATG	9 cut(s)	106, 1486, 1650, 1762, 1794, 1831, 2573, 3222, 4036
BstFNI	CGCG	2 cut(s)	127, 4122
BstH2I	RGCGCY	1 cut(s)	1961
BstHHI	GCGC	6 cut(s)	121, 129, 227, 1549, 1960, 4124
BstMAI	GTCTC	5 cut(s)	201, 358, 939, 3497, 3780
BstNI	CCWGG	8 cut(s)	324, 781, 1268, 2268, 2519, 2651, 3585, 3852
BstNSI	RCATGY	3 cut(s)	974, 2297, 2590
BstPI	GGTNACC	1 cut(s)	3911
BstSCI	CCNGG	9 cut(s)	263, 322, 779, 1266, 2266, 2517, 2649, 3583, 3850
BstSFI	CTRYAG	3 cut(s)	543, 984, 1105
BstUI	CGCG	2 cut(s)	127, 4122
BstV1I	GCAGC	6 cut(s)	133, 761, 1592, 1678, 2262, 2770
BstV2I	GAAGAC	6 cut(s)	126, 2033, 2096, 2837, 3225, 3557
BstX2I	RGATCY	7 cut(s)	241, 1531, 1851, 2161, 2392, 2545, 3982
BstYI	RGATCY	7 cut(s)	241, 1531, 1851, 2161, 2392, 2545, 3982
BstZ17I	GTATAC	2 cut(s)	104, 2929
Bsu36I	CCTNAGG	1 cut(s)	3900
BsuRI	GGCC	7 cut(s)	188, 884, 2250, 2476, 3583, 3933, 4008
BtgI	CCRYGG	2 cut(s)	189, 237
BtrI	CACGTC	3 cut(s)	1811, 3078, 3857
BtsCI	GGATG	9 cut(s)	106, 1486, 1650, 1762, 1794, 1831, 2573, 3222, 4036
BtsIMutI	CAGTG	4 cut(s)	117, 2203, 2910, 4024
BveI	ACCTGC	4 cut(s)	289, 1134, 1619, 2845
Cac8I	GCNNGC	8 cut(s)	117, 186, 261, 1612, 1739, 2252, 4006, 4120
CaiI	CAGNNNCTG	5 cut(s)	40, 329, 656, 2353, 2999
CfoI	GCGC	6 cut(s)	121, 129, 227, 1549, 1960, 4124
Cfr10I	RCCGGY	1 cut(s)	3925
Cfr13I	GGNCC	4 cut(s)	1123, 2474, 2731, 3948
CseI	GACGC	1 cut(s)	2090
Csp6I	GTAC	9 cut(s)	230, 506, 715, 1133, 1259, 1500, 2228, 2491, 2707
CspAI	ACCGGT	1 cut(s)	3925
CspCI	CAANNNNNGTGG	4 cut(s)	2931, 2966, 3516, 3551
CviQI	GTAC	9 cut(s)	230, 506, 715, 1133, 1259, 1500, 2228, 2491, 2707
DraI	TTTAAA	1 cut(s)	2139
DrdI	GACNNNNNNGTC	1 cut(s)	4105
DseDI	GACNNNNNNGTC	1 cut(s)	4105
EaeI	YGGCCR	1 cut(s)	186
Eam1104I	CTCTTC	2 cut(s)	455, 4032
EarI	CTCTTC	2 cut(s)	455, 4032
EciI	GGCGGA	1 cut(s)	1902
Ecl136II	GAGCTC	2 cut(s)	467, 802
Eco130I	CCWWGG	7 cut(s)	189, 879, 1056, 1426, 2710, 2734, 3578
Eco147I	AGGCCT	1 cut(s)	4008
Eco24I	GRGCYC	3 cut(s)	469, 804, 1213
Eco31I	GGTCTC	2 cut(s)	201, 358
Eco32I	GATATC	2 cut(s)	639, 1183
Eco47I	GGWCC	3 cut(s)	1123, 2731, 3948
Eco53kI	GAGCTC	2 cut(s)	467, 802
Eco72I	CACGTG	1 cut(s)	1703
Eco81I	CCTNAGG	1 cut(s)	3900
Eco91I	GGTNACC	1 cut(s)	3911
EcoICRI	GAGCTC	2 cut(s)	467, 802
EcoNI	CCTNNNNNAGG	1 cut(s)	2324
EcoO109I	RGGNCCY	1 cut(s)	1123
EcoO65I	GGTNACC	1 cut(s)	3911
EcoRI	GAATTC	2 cut(s)	1839, 3360
EcoRII	CCWGG	8 cut(s)	322, 779, 1266, 2266, 2517, 2649, 3583, 3850
EcoRV	GATATC	2 cut(s)	639, 1183
EcoT14I	CCWWGG	7 cut(s)	189, 879, 1056, 1426, 2710, 2734, 3578
EcoT22I	ATGCAT	3 cut(s)	1566, 2581, 3754
EcoT38I	GRGCYC	3 cut(s)	469, 804, 1213
ErhI	CCWWGG	7 cut(s)	189, 879, 1056, 1426, 2710, 2734, 3578
FaqI	GGGAC	1 cut(s)	1845
FauI	CCCGC	1 cut(s)	2654
FauNDI	CATATG	1 cut(s)	358
FbaI	TGATCA	2 cut(s)	253, 4176
FblI	GTMKAC	3 cut(s)	103, 1799, 2928
Fnu4HI	GCNGC	9 cut(s)	122, 125, 598, 750, 885, 1581, 1667, 2276, 2784
FokI	GGATG	9 cut(s)	93, 1493, 1657, 1749, 1801, 1838, 2580, 3209, 4043
FriOI	GRGCYC	3 cut(s)	469, 804, 1213
Fsp4HI	GCNGC	9 cut(s)	122, 125, 598, 750, 885, 1581, 1667, 2276, 2784
FspBI	CTAG	8 cut(s)	336, 1314, 1929, 2510, 2636, 3098, 3188, 3918
GlaI	GCGC	6 cut(s)	120, 128, 226, 1548, 1959, 4123
GluI	GCNGC	9 cut(s)	122, 125, 598, 750, 885, 1581, 1667, 2276, 2784
GsaI	CCCAGC	2 cut(s)	2475, 3230
GsuI	CTGGAG	7 cut(s)	14, 306, 788, 942, 1251, 1556, 1727
HaeII	RGCGCY	1 cut(s)	1961
HaeIII	GGCC	7 cut(s)	188, 884, 2250, 2476, 3583, 3933, 4008
HapII	CCGG	2 cut(s)	264, 3926
HgaI	GACGC	1 cut(s)	2090
HhaI	GCGC	6 cut(s)	121, 129, 227, 1549, 1960, 4124
Hin1I	GRCGYC	1 cut(s)	3024
Hin6I	GCGC	6 cut(s)	119, 127, 225, 1547, 1958, 4122
HinP1I	GCGC	6 cut(s)	119, 127, 225, 1547, 1958, 4122
HincII	GTYRAC	1 cut(s)	3519
HindII	GTYRAC	1 cut(s)	3519
HindIII	AAGCTT	2 cut(s)	2720, 3111
HpaII	CCGG	2 cut(s)	264, 3926
HphI	GGTGA	9 cut(s)	25, 377, 425, 1127, 1499, 2981, 3599, 3608, 3989
Hpy99I	CGWCG	2 cut(s)	3079, 3796
HpyCH4IV	ACGT	7 cut(s)	1702, 1810, 2038, 3024, 3077, 3316, 3856
HpySE526I	ACGT	7 cut(s)	1702, 1810, 2038, 3024, 3077, 3316, 3856
Hsp92I	GRCGYC	1 cut(s)	3024
HspAI	GCGC	6 cut(s)	119, 127, 225, 1547, 1958, 4122
Ksp22I	TGATCA	2 cut(s)	253, 4176
LmnI	GCTCC	7 cut(s)	500, 694, 807, 1746, 2350, 3305, 3842
Lsp1109I	GCAGC	6 cut(s)	133, 761, 1592, 1678, 2262, 2770
LweI	GCATC	8 cut(s)	301, 1471, 1573, 1597, 1915, 3184, 3549, 4021
MaeI	CTAG	8 cut(s)	336, 1314, 1929, 2510, 2636, 3098, 3188, 3918
MaeII	ACGT	7 cut(s)	1702, 1810, 2038, 3024, 3077, 3316, 3856
MflI	RGATCY	7 cut(s)	241, 1531, 1851, 2161, 2392, 2545, 3982
MhlI	GDGCHC	4 cut(s)	469, 804, 1213, 2203
MlsI	TGGCCA	1 cut(s)	188
MluNI	TGGCCA	1 cut(s)	188
MlyI	GAGTC	6 cut(s)	362, 2177, 2681, 3080, 3782, 3887
MmeI	TCCRAC	8 cut(s)	1221, 1786, 2203, 2860, 3283, 3337, 3774, 3820
Mox20I	TGGCCA	1 cut(s)	188
Mph1103I	ATGCAT	3 cut(s)	1566, 2581, 3754
MroXI	GAANNNNTTC	3 cut(s)	2040, 2979, 3402
MscI	TGGCCA	1 cut(s)	188
MslI	CAYNNNNRTG	4 cut(s)	387, 438, 2154, 2501
Msp20I	TGGCCA	1 cut(s)	188
MspA1I	CMGCKG	2 cut(s)	1669, 2783
MspCI	CTTAAG	1 cut(s)	3862
MspI	CCGG	2 cut(s)	264, 3926
MspR9I	CCNGG	9 cut(s)	265, 324, 781, 1268, 2268, 2519, 2651, 3585, 3852
Mva1269I	GAATGC	5 cut(s)	69, 247, 1389, 3579, 3752
MvaI	CCWGG	8 cut(s)	324, 781, 1268, 2268, 2519, 2651, 3585, 3852
MvnI	CGCG	2 cut(s)	127, 4122
NciI	CCSGG	1 cut(s)	265
NcoI	CCATGG	1 cut(s)	189
NdeI	CATATG	1 cut(s)	358
NlaIV	GGNNCC	5 cut(s)	243, 445, 496, 849, 1245
NmeAIII	GCCGAG	1 cut(s)	3832
NmuCI	GTSAC	5 cut(s)	365, 431, 1138, 1487, 1979
NsiI	ATGCAT	3 cut(s)	1566, 2581, 3754
NspI	RCATGY	3 cut(s)	974, 2297, 2590
OliI	CACNNNNGTG	1 cut(s)	2154
PasI	CCCWGGG	1 cut(s)	1267
PceI	AGGCCT	1 cut(s)	4008
PciI	ACATGT	1 cut(s)	2293
PctI	GAATGC	5 cut(s)	69, 247, 1389, 3579, 3752
PdmI	GAANNNNTTC	3 cut(s)	2040, 2979, 3402
PfoI	TCCNGGA	2 cut(s)	2266, 3850
PinAI	ACCGGT	1 cut(s)	3925
PkrI	GCNGC	9 cut(s)	123, 126, 599, 751, 886, 1582, 1668, 2277, 2785
PleI	GAGTC	6 cut(s)	362, 2176, 2680, 3080, 3782, 3887
PmaCI	CACGTG	1 cut(s)	1703
PmlI	CACGTG	1 cut(s)	1703
PpsI	GAGTC	6 cut(s)	362, 2176, 2680, 3080, 3782, 3887
Ppu21I	YACGTR	2 cut(s)	1703, 3317
PpuMI	RGGWCCY	1 cut(s)	1123
PscI	ACATGT	1 cut(s)	2293
Psp124BI	GAGCTC	2 cut(s)	469, 804
Psp5II	RGGWCCY	1 cut(s)	1123
Psp6I	CCWGG	8 cut(s)	322, 779, 1266, 2266, 2517, 2649, 3583, 3850
PspCI	CACGTG	1 cut(s)	1703
PspEI	GGTNACC	1 cut(s)	3911
PspFI	CCCAGC	2 cut(s)	2471, 3226
PspGI	CCWGG	8 cut(s)	322, 779, 1266, 2266, 2517, 2649, 3583, 3850
PspN4I	GGNNCC	5 cut(s)	243, 445, 496, 849, 1245
PspPI	GGNCC	4 cut(s)	1123, 2474, 2731, 3948
PspPPI	RGGWCCY	1 cut(s)	1123
PstNI	CAGNNNCTG	5 cut(s)	40, 329, 656, 2353, 2999
PsuI	RGATCY	7 cut(s)	241, 1531, 1851, 2161, 2392, 2545, 3982
PvuII	CAGCTG	2 cut(s)	1669, 2783
RsaI	GTAC	9 cut(s)	231, 507, 716, 1134, 1260, 1501, 2229, 2492, 2708
RsaNI	GTAC	9 cut(s)	230, 506, 715, 1133, 1259, 1500, 2228, 2491, 2707
RseI	CAYNNNNRTG	4 cut(s)	387, 438, 2154, 2501
SacI	GAGCTC	2 cut(s)	469, 804
SatI	GCNGC	9 cut(s)	122, 125, 598, 750, 885, 1581, 1667, 2276, 2784
Sau96I	GGNCC	4 cut(s)	1123, 2474, 2731, 3948
SchI	GAGTC	6 cut(s)	362, 2177, 2681, 3080, 3782, 3887
ScrFI	CCNGG	9 cut(s)	265, 324, 781, 1268, 2268, 2519, 2651, 3585, 3852
SduI	GDGCHC	4 cut(s)	469, 804, 1213, 2203
SfaNI	GCATC	8 cut(s)	301, 1471, 1573, 1597, 1915, 3184, 3549, 4021
SfcI	CTRYAG	3 cut(s)	543, 984, 1105
SinI	GGWCC	3 cut(s)	1123, 2731, 3948
SmiMI	CAYNNNNRTG	4 cut(s)	387, 438, 2154, 2501
SmlI	CTYRAG	3 cut(s)	2165, 2336, 3862
SmoI	CTYRAG	3 cut(s)	2165, 2336, 3862
SseBI	AGGCCT	1 cut(s)	4008
SspI	AATATT	3 cut(s)	25, 3094, 3322
SspMI	CTAG	8 cut(s)	336, 1314, 1929, 2510, 2636, 3098, 3188, 3918
SstI	GAGCTC	2 cut(s)	469, 804
StuI	AGGCCT	1 cut(s)	4008
StyD4I	CCNGG	9 cut(s)	263, 322, 779, 1266, 2266, 2517, 2649, 3583, 3850
StyI	CCWWGG	7 cut(s)	189, 879, 1056, 1426, 2710, 2734, 3578
TaiI	ACGT	7 cut(s)	1705, 1813, 2041, 3027, 3080, 3319, 3859
TaqI	TCGA	5 cut(s)	1774, 1843, 2809, 3499, 4137
TatI	WGTACW	5 cut(s)	229, 714, 1258, 2227, 2490
TauI	GCSGC	3 cut(s)	127, 600, 887
TscAI	CASTG	4 cut(s)	117, 2203, 2917, 4024
TseFI	GTSAC	5 cut(s)	365, 431, 1138, 1487, 1979
TseI	GCWGC	6 cut(s)	121, 749, 1580, 1666, 2275, 2783
Tsp45I	GTSAC	5 cut(s)	365, 431, 1138, 1487, 1979
TspGWI	ACGGA	5 cut(s)	254, 493, 1075, 1801, 2759
TspRI	CASTG	4 cut(s)	117, 2203, 2917, 4024
Vha464I	CTTAAG	1 cut(s)	3862
VpaK11BI	GGWCC	3 cut(s)	1123, 2731, 3948
XagI	CCTNNNNNAGG	1 cut(s)	2324
XapI	RAATTY	6 cut(s)	152, 672, 1839, 2979, 3360, 3447
XbaI	TCTAGA	1 cut(s)	2509
XceI	RCATGY	3 cut(s)	974, 2297, 2590
XmiI	GTMKAC	3 cut(s)	103, 1799, 2928
XmnI	GAANNNNTTC	3 cut(s)	2040, 2979, 3402
XspI	CTAG	8 cut(s)	336, 1314, 1929, 2510, 2636, 3098, 3188, 3918
ZraI	GACGTC	1 cut(s)	3025
Zsp2I	ATGCAT	3 cut(s)	1566, 2581, 3754

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.