Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0005249

Physical Location & Seq

Genomic Coordinates Forward (+)

6834077 .. 6844689

Gene Structure 10613 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1097800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4215 bp

ATGAGCAATTCCGTAGGCCATAACTTGCTCAATCAGAGCTTGTTTCAAAGCAAGATAAATTCTTCTGGTATTCCTGCCAACACATTGTTTCAAGCTAAGAGCGTCCACCAAGTGACAGCTCAGGCGCGAAAATCACCCATATCCAAGAAATTCTGTGGGAACACTTTGAACGTTCAGAAACCAAAGTTAGCCATGGGGTCATGCCGCCCCATTACAGCTATTCCTCTTGCCGTATTAACCACTGATCCGCCTTCCAATCAGCAGCTTGCTGGGAAATTCAATCTCGATGGGAATATTGAAATGCAGGTTTATGTGAATCCTTCCACACCGGGATCTGCCACTCAAGTAGATATCTGTGTAGCATATAGCGGTGACTCTTTGACTCTTCACTGGGGTGGGATAAAAGATAGAAAAGAAAAATGGGTGCTTCCATCTCGTCACCCAGATGGAACCAAAGTATATAAGAACAAAGCTCTTAGAACACCTTTTGTGAATTCCGGCTCCAATTCCTTGCTTCAAATTGAGATTGATGAGCCTACAATACAAGCAATCGAATTTCTTTTAGTTGATGAAAGCCAGAACAGATGGTTTAAGAACAATGGTGATAACTTTCATGTTAAGTTACCTGCAAAAGAGAAGTTGATATCCAATGCTTCAGTTCCTGAAGAGCTTGTACAAATTCAAGCATATTTGAGGTGGGAAAGAAAGGGTAAACAGATGTATACACCAGAGCAAGAAAAGGTGGAGTACGAAGCAGCCCGAACTGAGTTATTGGAGGAAGTAGCTAGGGGTACTTCCATACAAGAGCTCCAAGCAAGGTTGACTAAGAAAAATGATGGTGGTAAGCTTGAAGAGCCATCTGGTTCTGATACAAAGGCTGCAAAAAAATCTGAAAGCAAAAAGGTTAGAACCGAGAGAATTCAACGCAAGACGAGGGACTTAACGCGGATTATCAACAAACATGCTACTAAACCTGTTGATGAAGCTAAATTTGTAGATGAGAAACATCCAACCAAACCAAAACCCTTGACAGCAGTTGAGCTTTTCGCCAAGGAAAAGGAAGAACGGGATGGAGGTTCTGTTCTGAGGAAAAACATCTTCAAGATCAATGATAAGGAACTTCTGGTACTTGTAACCAAGCCTGCTGGGAAGACAAAGGTTCATCTGGCAACAGATTTCAAAGAGCCACTTGCCCTTCACTGGGCTTTATCTGCAAGCAAGGCTGGAGAGTGGTCGGAACCACCTGCAAGTACACATCCCCAGGGTTCAGTTGCTCTAAATGGGGCTGTTGAAACACAATTTAGACCAAGTTTCACTGACTCTACTTATGAGGTTCAATCTATAGAAATAGAAATTGAAGGAGAGAGTTTTAAAGGAATGCCGTTTGTCCTTAATGCTTCTGGGAACTGGATGAAGAATCAAGGCTCCGATTTCTACATTGACTTTGGTGTCGAACGCAAGAAAGCTCAGAAGGATACTGGTGATGGCAAGGGTACTGCAAAGGCTTTGTTGGATAAAATAGCCGACCAGGAGAGTGAGGCACAGAAATCCTTTATGCATCGATTTAACATTGCAGCAGACTTGGTCAATCAAGCAAAAGATGCTGGTGAATTAGGTCTTGCAGGTATATTGGTGTGGATGAGGTTTATGGCTACGAGGCAGCTCATTTGGAATAAAAATTACAATGTGAAACCACGTGAGATTAGTAAAGCACAGGACAGGCTCACGGACTTGCTCCAAAATGTTTATGCAAATTATCCACAGTATCGCGAACTTCTACGGATGATCTTGTCTACCGTTGGCCGTGGAGGAGAAGGGGATGTGGGACAGCGAATTCGAGATGAGATCCTTGTTATCCAGAGAAAGAATAATTGCAAAGGTGGAATGATGGAGGAATGGCATCAAAAGTTGCATAATAACACAAGTCCAGATGATGTTGTTATTTGTCAGGCATTAATAGATTACATAAAAAATGACTTTGACATTGGTGTTTACTGGAAAACTTTGAATGAGAATGGAATCACAAAAGAACGTCTCCTGAGCTACGATCGTGCAATCCGTAATGAACCAAATTTCAGGAGTGATCAGAAAGATGGTCTACTGCGTGATCTGGGGCACTACATGAGAACTTTGAAGGCTGTTCATTCAGGTGCAGATCTTGAGTCTGCCATTCAAAATTGTATGGGCTACAAAGATGAGGGTCAAGGATTCATGGTTGGAGTACAAATAAATCCTATATCTGGCTTGCCATCTGAAGTTCCAGACCTCCTGCGATTTGTACTAGAACATGTTGAGGACAAGAATGTGGAAGTCCTTATTGAGGGTTTGCTTGAGGCGCGCCAGGCGCTTTGGCCATTGCTCTCTAAGCCTCATGATCGTCTGAGGGATCTTTTATTTTTAGACATTGCCCTGGATTCTACTGTTAGGACCGCAATTGAGAGGGGATATGAAGAACTGAATAATGCTGGACCAGAGAAAATTATGTACTTCATCTCTTTGGTTCTAGAAAACCTGGCCCTATCATCAGACAATAACGAAGATCTCATTTACTGTTTGAAGGGATGGGATCAGGCTCAAAACATGTTAAAAAGTAAAAGCAATGACTGGGCATTATATTCAAAATCAATCCTAGACAGAACCCGCCTTGCACTTGCAAACAAGGCAGAATCGTATCTCAGAATCTTACAGCCATCAGCAGAATACCTCGGAGCACAGCTTGGAGTGGACCAATGGGCTGTGAACATATTTACTGAAGAAATAATCCGTGCTGGATCAGCTGCATCTTTATCCTCACTCCTGAATCGGCTTGATCCGGTTCTTCGAAAGACTGCTAATATGGGAAGTTGGCAGGTTATCAGCCCAGTTGAAGTGGTTGGATATGTCGTCTATGTGGATGAATTGCTCACTGTTCAGAATAAAGTTTACAGCAAGCCCACAATTTTGGTGGCAAAAAGTGTAAAAGGCGAGGAAGAAATTCCAGATGGCACTGTTGCTGTTCTGACACCAGATATGCCAGACGTCCTATCTCATGTCTCTGTCCGAGCAAGAAATAGCAAGGTTTGTTTTGCGACATGCTTTGACCCCAATATTCTGGCTGACCTCCAAGCTAGTGAGGGGAAATTATTACGTCTAAAGCCAACACCTGCTGATATAGTTTATAGTGAGGTCAATGAGGGTGAGCTAGATGCAGGTTCAACTGATTCAAAAGAAGACACTCCATCTCTGACATTGGTCAAGAAAAAATTTACCGGTAGATATGCCATATCATCTGATGAGTTCACAAGCGAAATGGTTGGAGCTAAATCACGTAATATGGCTTATGTAAAAGGAAAACTTCCATCTTGGGTTGGAATTCCTACATCGGTTGCCCTACCATTTGGATCATTTGAGAAGGTTCTTTCAGAAGATTCAAACAAGGCAGTGGCTGAAAAGTTGGAAACTCTAAAGAAAAAGTTGAAAGACGGAGATTTTGATTCGCTCAAGAAGATTCGGGAAACAGTTTTACAGCTGGCAGCACCACACCAGTTGGTGCAAGAGCTGAAGACTACAATGAAGGGGTCAGGGATGCCTTGGCCTGGTGATGAAGGTGAACAACGGTGGGAACAAGCATGGACGGCTATAAAAAAGGTCTGGGCTTCAAAGTGGAACGAGAGAGCGTACTTCAGCACGAGAAAAGTGAAATTAGATCACGACTATCTTTGCATGGCTGTCCTTGTTCAGGAAATCATAAACGCTGACTATGCATTTGTTATTCACACAACTAACCCATCTTCAGGAGACTCATCAGAGATATATGCTGAGGTTGTTAAGGGGCTTGGAGAAACCCTAGTTGGAGCTTATCCTGGACGTGCTCTAAGTTTTATTAGCAAAAAGAATGATCTGGACTCTCCTCAGGTGTTAGGATACCCTAGCAAACCGATTGGCCTCTTTATAAAACGGTCTATAATCTTTCGATCCGATTCCAATGGTGAAGATCTCGAAGGTTATGCTGGTGCAGGTCTTTACGACAGTGTACCCATGGATGAGGAAGAGAAAGTCGTGGTTGACTACTCATCTGACCCGCTGATGGTTGACGGAAACTTCCGCAAGACAATCCTCTCTAGCATTGCTCGTGCAGGGAGCGCAATCGAGGAACTGTATGGATCTCCACAAGACATTGAAGGTGTGATCAGGGACGGTAAACTCTATGTTGTCCAGACAAGACCCCAAATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009628 GO:0009631 GO:0009941 GO:0009987 GO:0016052 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016781 GO:0031967 GO:0031975 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050521 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPDK_N

Protein Analysis

1405

Amino Acids

156.57

Weight (kDa)

6.33

Isoelectric Point (pI)

36.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ig_N_CWD1	PF23166	90 - 208	2.8e-40	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
DUF7067	PF23229	222 - 277	9.3e-28	Alpha-glucan water dikinase, DUF7067
Ig_N_CWD1	PF23166	365 - 482	9.8e-29	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
GWD1_pHisD	PF22973	948 - 1060	3.8e-53	Alpha-glucan water dikinase, phosphohistidine-like domain
PPDK_N	PF01326	1201 - 1403	7e-21	Pyruvate phosphate dikinase, AMP/ATP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10760 AT1G10760 AT1G10760
fragaria_vesca	FvH4_4g25800 FvH4_4g25800 FvH4_4g25800 FvH4_4g25800
malus_domestica	MD13G1096800.v1.1 MD16G1097800.v1.1
prunus_persica	Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1
pyrus_communis	pycom13g08450 pycom16g08330
rosa_chinensis	RchiOBHm_Chr4g0433251
rosa_laevigata	RLG00000006771
rosa_multiflora	Rmu_co8055492.1_g000001 Rmu_sc0010643.1_g000001
rosa_roxburghii	Rroxscaffold_5G00374810
rosa_rugosa	Rorug04G0267200 Rorug04G0267300 Rorug04G0267400 Rorug04G0267400
rosa_samantha	Rh4AG321900 Rh4BG329300 Rh4CG344600
rosa_wichuraiana	Rw4G027860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3932
AarI	CACCTGC	2 cut(s)	1252, 3151
AasI	GACNNNNNNGTC	1 cut(s)	1448
AatII	GACGTC	1 cut(s)	3021
Acc36I	ACCTGC	8 cut(s)	295, 634, 1252, 1613, 2839, 3151, 3179, 3986
AccI	GTMKAC	3 cut(s)	722, 1793, 2098
AccII	CGCG	4 cut(s)	127, 946, 1770, 2338
AciI	CCGC	8 cut(s)	205, 248, 369, 946, 2430, 2641, 4061, 4084
AclI	AACGTT	1 cut(s)	171
AcoI	YGGCCR	2 cut(s)	1801, 2351
AcuI	CTGAAG	7 cut(s)	639, 684, 2274, 2772, 3560, 3646, 3756
AcvI	CACGTG	1 cut(s)	1697
AcyI	GRCGYC	1 cut(s)	3018
AdeI	CACNNNGTG	1 cut(s)	112
AfiI	CCNNNNNNNGG	8 cut(s)	1200, 1201, 2240, 2320, 3575, 3718, 3773, 4066
AflIII	ACRYGT	2 cut(s)	2287, 2580
AgeI	ACCGGT	1 cut(s)	3248
AjiI	CACGTC	1 cut(s)	3848
AjnI	CCWGG	7 cut(s)	1260, 1527, 2340, 2409, 2511, 3574, 3841
AjuI	GAANNNNNNNTTGG	2 cut(s)	3813, 3845
AleI	CACNNNNGTG	1 cut(s)	393
AloI	GAACNNNNNNTCC	2 cut(s)	1065, 1097
Alw21I	GWGCWC	3 cut(s)	810, 2713, 3853
Alw26I	GTCTC	3 cut(s)	2039, 3037, 3771
AlwNI	CAGNNNCTG	2 cut(s)	662, 3425
AoxI	GGCC	6 cut(s)	16, 1801, 2351, 2514, 3572, 3922
ApeKI	GCWGC	7 cut(s)	262, 755, 878, 1574, 1660, 2777, 3512
ArsI	GACNNNNNNTTYG	6 cut(s)	1818, 1850, 3452, 3484, 3728, 3760
AscI	GGCGCGCC	1 cut(s)	2336
AseI	ATTAAT	1 cut(s)	1955
AsiGI	ACCGGT	1 cut(s)	3248
Asp700I	GAANNNNTTC	1 cut(s)	2973
AspLEI	GCGC	5 cut(s)	127, 2338, 2340, 2347, 4124
AspS9I	GGNCC	4 cut(s)	2427, 2468, 2515, 2725
AsuC2I	CCSGG	1 cut(s)	330
AsuII	TTCGAA	1 cut(s)	2821
AvaII	GGWCC	3 cut(s)	2427, 2468, 2725
AxyI	CCTNAGG	1 cut(s)	3891
BaeGI	GKGCMC	1 cut(s)	2118
BalI	TGGCCA	1 cut(s)	2353
BanII	GRGCYC	1 cut(s)	810
BarI	GAAGNNNNNNTAC	2 cut(s)	657, 689
BauI	CACGAG	2 cut(s)	3667, 4110
BbrPI	CACGTG	1 cut(s)	1697
BbsI	GAAGAC	3 cut(s)	1157, 3216, 3548
Bbv12I	GWGCWC	3 cut(s)	810, 2713, 3853
BbvCI	CCTCAGC	1 cut(s)	3798
BbvI	GCAGC	7 cut(s)	274, 767, 865, 1586, 1672, 2764, 3524
BceAI	ACGGC	4 cut(s)	215, 1366, 1788, 3630
BcgI	CGANNNNNNTGC	2 cut(s)	3968, 4002
BciT130I	CCWGG	7 cut(s)	1262, 1529, 2342, 2411, 2513, 3576, 3843
BciVI	GTATCC	2 cut(s)	1468, 3896
BclI	TGATCA	2 cut(s)	2083, 4167
BcnI	CCSGG	1 cut(s)	330
BcoDI	GTCTC	3 cut(s)	2039, 3037, 3771
BfaI	CTAG	9 cut(s)	786, 2282, 2504, 2630, 3108, 3182, 3827, 3909, 4101
BfmI	CTRYAG	1 cut(s)	1341
BfoI	RGCGCY	1 cut(s)	2348
BfuAI	ACCTGC	8 cut(s)	295, 634, 1252, 1613, 2839, 3151, 3179, 3986
BfuI	GTATCC	2 cut(s)	1468, 3896
BglII	AGATCT	3 cut(s)	2155, 2539, 3973
BisI	GCNGC	8 cut(s)	205, 263, 756, 879, 1575, 1661, 2778, 3513
BlsI	GCNGC	8 cut(s)	206, 264, 757, 880, 1576, 1662, 2779, 3514
Bme1390I	CCNGG	8 cut(s)	330, 1262, 1529, 2342, 2411, 2513, 3576, 3843
Bme18I	GGWCC	3 cut(s)	2427, 2468, 2725
BmgBI	CACGTC	1 cut(s)	3848
BmgT120I	GGNCC	4 cut(s)	2427, 2468, 2515, 2725
BmiI	GGNNCC	4 cut(s)	451, 502, 1239, 1426
BmrFI	CCNGG	8 cut(s)	330, 1262, 1529, 2342, 2411, 2513, 3576, 3843
BmrI	ACTGGG	4 cut(s)	400, 1210, 2614, 2855
BmsI	GCATC	6 cut(s)	1567, 1591, 1909, 2789, 3175, 3555
BmuI	ACTGGG	4 cut(s)	400, 1210, 2614, 2855
BoxI	GACNNNNGTC	1 cut(s)	3230
BpiI	GAAGAC	3 cut(s)	1157, 3216, 3548
BpmI	CTGGAG	1 cut(s)	1245
Bpu10I	CCTNAGC	3 cut(s)	120, 2039, 3798
Bpu14I	TTCGAA	1 cut(s)	2821
BpuEI	CTTGAG	4 cut(s)	327, 2180, 2351, 3464
BpuMI	CCSGG	1 cut(s)	330
Bsa29I	ATCGAT	1 cut(s)	1561
BsaAI	YACGTR	2 cut(s)	1697, 3308
BsaHI	GRCGYC	1 cut(s)	3018
BsaJI	CCNNGG	9 cut(s)	192, 1050, 1260, 1261, 1804, 2409, 2704, 3569, 4017
BsaWI	WCCGGW	2 cut(s)	2812, 3248
BsaXI	ACNNNNNCTCC	4 cut(s)	792, 822, 1065, 1095
Bsc4I	CCNNNNNNNGG	8 cut(s)	1200, 1201, 2240, 2320, 3575, 3718, 3773, 4066
Bse118I	RCCGGY	1 cut(s)	3248
Bse1I	ACTGG	8 cut(s)	395, 1205, 1412, 1483, 2000, 2609, 2861, 3523
Bse21I	CCTNAGG	1 cut(s)	3891
Bse3DI	GCAATG	5 cut(s)	1569, 2354, 2403, 2605, 4104
BseBI	CCWGG	7 cut(s)	1262, 1529, 2342, 2411, 2513, 3576, 3843
BseCI	ATCGAT	1 cut(s)	1561
BseDI	CCNNGG	9 cut(s)	192, 1050, 1260, 1261, 1804, 2409, 2704, 3569, 4017
BseLI	CCNNNNNNNGG	8 cut(s)	1200, 1201, 2240, 2320, 3575, 3718, 3773, 4066
BseMI	GCAATG	5 cut(s)	1569, 2354, 2403, 2605, 4104
BseMII	CTCAG	9 cut(s)	134, 756, 1076, 1481, 2030, 2372, 2689, 3789, 3905
BseNI	ACTGG	8 cut(s)	395, 1205, 1412, 1483, 2000, 2609, 2861, 3523
BsePI	GCGCGC	1 cut(s)	2336
BseRI	GAGGAG	2 cut(s)	1824, 3879
BseSI	GKGCMC	1 cut(s)	2118
BseXI	GCAGC	7 cut(s)	274, 767, 865, 1586, 1672, 2764, 3524
BseYI	CCCAGC	2 cut(s)	269, 1145
BsgI	GTGCAG	3 cut(s)	2172, 4014, 4134
Bsh1236I	CGCG	4 cut(s)	127, 946, 1770, 2338
Bsh1285I	CGRYCG	1 cut(s)	2050
BshFI	GGCC	6 cut(s)	18, 1803, 2353, 2516, 3574, 3924
BshTI	ACCGGT	1 cut(s)	3248
BshVI	ATCGAT	1 cut(s)	1561
BsiEI	CGRYCG	1 cut(s)	2050
BsiHKAI	GWGCWC	3 cut(s)	810, 2713, 3853
BsiSI	CCGG	4 cut(s)	329, 498, 2813, 3249
BslFI	GGGAC	3 cut(s)	950, 1839, 4187
BslI	CCNNNNNNNGG	8 cut(s)	1200, 1201, 2240, 2320, 3575, 3718, 3773, 4066
BsmAI	GTCTC	3 cut(s)	2039, 3037, 3771
BsmBI	CGTCTC	1 cut(s)	2039
BsmFI	GGGAC	3 cut(s)	950, 1839, 4187
BsmI	GAATGC	1 cut(s)	1383
BsnI	GGCC	6 cut(s)	18, 1803, 2353, 2516, 3574, 3924
Bsp119I	TTCGAA	1 cut(s)	2821
Bsp1286I	GDGCHC	4 cut(s)	810, 2118, 2713, 3853
Bsp1407I	TGTACA	1 cut(s)	673
Bsp19I	CCATGG	2 cut(s)	192, 4017
Bsp68I	TCGCGA	1 cut(s)	1770
BspACI	CCGC	8 cut(s)	205, 248, 369, 946, 2430, 2641, 4061, 4084
BspANI	GGCC	6 cut(s)	18, 1803, 2353, 2516, 3574, 3924
BspCNI	CTCAG	9 cut(s)	133, 757, 1077, 1480, 2031, 2373, 2688, 3790, 3904
BspDI	ATCGAT	1 cut(s)	1561
BspFNI	CGCG	4 cut(s)	127, 946, 1770, 2338
BspHI	TCATGA	1 cut(s)	2371
BspLI	GGNNCC	4 cut(s)	451, 502, 1239, 1426
BspMI	ACCTGC	8 cut(s)	295, 634, 1252, 1613, 2839, 3151, 3179, 3986
BspQI	GCTCTTC	2 cut(s)	660, 846
BspT104I	TTCGAA	1 cut(s)	2821
BsrDI	GCAATG	5 cut(s)	1569, 2354, 2403, 2605, 4104
BsrFI	RCCGGY	1 cut(s)	3248
BsrGI	TGTACA	1 cut(s)	673
BsrI	ACTGG	8 cut(s)	395, 1205, 1412, 1483, 2000, 2609, 2861, 3523
BssAI	RCCGGY	1 cut(s)	3248
BssECI	CCNNGG	9 cut(s)	192, 1050, 1260, 1261, 1804, 2409, 2704, 3569, 4017
BssHII	GCGCGC	1 cut(s)	2336
BssNAI	GTATAC	1 cut(s)	723
BssNI	GRCGYC	1 cut(s)	3018
BssSI	CACGAG	2 cut(s)	3667, 4110
BssT1I	CCWWGG	4 cut(s)	192, 1050, 3569, 4017
Bst1107I	GTATAC	1 cut(s)	723
Bst2BI	CACGAG	2 cut(s)	3667, 4110
Bst2UI	CCWGG	7 cut(s)	1262, 1529, 2342, 2411, 2513, 3576, 3843
Bst6I	CTCTTC	4 cut(s)	390, 660, 846, 4023
BstACI	GRCGYC	1 cut(s)	3018
BstAPI	GCANNNNNTGC	1 cut(s)	1601
BstAUI	TGTACA	1 cut(s)	673
BstBAI	YACGTR	2 cut(s)	1697, 3308
BstBI	TTCGAA	1 cut(s)	2821
BstC8I	GCNNGC	7 cut(s)	267, 1143, 1216, 2246, 2338, 2930, 3510
BstDSI	CCRYGG	3 cut(s)	192, 1804, 4017
BstENI	CCTNNNNNAGG	2 cut(s)	2318, 3716
BstFNI	CGCG	4 cut(s)	127, 946, 1770, 2338
BstH2I	RGCGCY	1 cut(s)	2348
BstHHI	GCGC	5 cut(s)	127, 2338, 2340, 2347, 4124
BstMAI	GTCTC	3 cut(s)	2039, 3037, 3771
BstMCI	CGRYCG	1 cut(s)	2050
BstNI	CCWGG	7 cut(s)	1262, 1529, 2342, 2411, 2513, 3576, 3843
BstNSI	RCATGY	4 cut(s)	965, 2291, 2584, 3075
BstPAI	GACNNNNGTC	1 cut(s)	3230
BstSCI	CCNGG	8 cut(s)	328, 1260, 1527, 2340, 2409, 2511, 3574, 3841
BstSFI	CTRYAG	1 cut(s)	1341
BstSLI	GKGCMC	1 cut(s)	2118
BstUI	CGCG	4 cut(s)	127, 946, 1770, 2338
BstV1I	GCAGC	7 cut(s)	274, 767, 865, 1586, 1672, 2764, 3524
BstV2I	GAAGAC	3 cut(s)	1157, 3216, 3548
BstX2I	RGATCY	7 cut(s)	332, 1845, 2155, 2386, 2539, 3973, 4142
BstXI	CCANNNNNNTGG	2 cut(s)	2941, 3091
BstYI	RGATCY	7 cut(s)	332, 1845, 2155, 2386, 2539, 3973, 4142
BstZ17I	GTATAC	1 cut(s)	723
Bsu15I	ATCGAT	1 cut(s)	1561
Bsu36I	CCTNAGG	1 cut(s)	3891
BsuI	GTATCC	2 cut(s)	1468, 3896
BsuRI	GGCC	6 cut(s)	18, 1803, 2353, 2516, 3574, 3924
BsuTUI	ATCGAT	1 cut(s)	1561
BtgI	CCRYGG	3 cut(s)	192, 1804, 4017
BtrI	CACGTC	1 cut(s)	3848
BtsI	GCAGTG	1 cut(s)	3426
BtsIMutI	CAGTG	8 cut(s)	240, 388, 1198, 1314, 2904, 2985, 3426, 4015
BtuMI	TCGCGA	1 cut(s)	1770
BveI	ACCTGC	8 cut(s)	295, 634, 1252, 1613, 2839, 3151, 3179, 3986
Cac8I	GCNNGC	7 cut(s)	267, 1143, 1216, 2246, 2338, 2930, 3510
CaiI	CAGNNNCTG	2 cut(s)	662, 3425
CciI	TCATGA	1 cut(s)	2371
CfoI	GCGC	5 cut(s)	127, 2338, 2340, 2347, 4124
Cfr10I	RCCGGY	1 cut(s)	3248
Cfr13I	GGNCC	4 cut(s)	2427, 2468, 2515, 2725
ClaI	ATCGAT	1 cut(s)	1561
CseI	GACGC	1 cut(s)	91
CspAI	ACCGGT	1 cut(s)	3248
CspCI	CAANNNNNGTGG	4 cut(s)	2925, 2960, 3507, 3542
DraI	TTTAAA	1 cut(s)	1372
DraIII	CACNNNGTG	1 cut(s)	112
DrdI	GACNNNNNNGTC	1 cut(s)	1448
DseDI	GACNNNNNNGTC	1 cut(s)	1448
EaeI	YGGCCR	2 cut(s)	1801, 2351
Eam1104I	CTCTTC	4 cut(s)	390, 660, 846, 4023
EarI	CTCTTC	4 cut(s)	390, 660, 846, 4023
EciI	GGCGGA	1 cut(s)	237
Ecl136II	GAGCTC	1 cut(s)	808
Eco130I	CCWWGG	4 cut(s)	192, 1050, 3569, 4017
Eco24I	GRGCYC	1 cut(s)	810
Eco32I	GATATC	2 cut(s)	352, 645
Eco47I	GGWCC	3 cut(s)	2427, 2468, 2725
Eco53kI	GAGCTC	1 cut(s)	808
Eco57I	CTGAAG	7 cut(s)	639, 684, 2274, 2772, 3560, 3646, 3756
Eco72I	CACGTG	1 cut(s)	1697
Eco81I	CCTNAGG	1 cut(s)	3891
EcoICRI	GAGCTC	1 cut(s)	808
EcoNI	CCTNNNNNAGG	2 cut(s)	2318, 3716
EcoRI	GAATTC	4 cut(s)	493, 918, 1833, 3351
EcoRII	CCWGG	7 cut(s)	1260, 1527, 2340, 2409, 2511, 3574, 3841
EcoRV	GATATC	2 cut(s)	352, 645
EcoT14I	CCWWGG	4 cut(s)	192, 1050, 3569, 4017
EcoT22I	ATGCAT	2 cut(s)	1560, 3745
EcoT38I	GRGCYC	1 cut(s)	810
ErhI	CCWWGG	4 cut(s)	192, 1050, 3569, 4017
Esp3I	CGTCTC	1 cut(s)	2039
FalI	AAGNNNNNCTT	4 cut(s)	1173, 1205, 3318, 3350
FaqI	GGGAC	3 cut(s)	950, 1839, 4187
FauI	CCCGC	2 cut(s)	2648, 4068
FbaI	TGATCA	2 cut(s)	2083, 4167
FblI	GTMKAC	3 cut(s)	722, 1793, 2098
Fnu4HI	GCNGC	8 cut(s)	205, 263, 756, 879, 1575, 1661, 2778, 3513
FriOI	GRGCYC	1 cut(s)	810
Fsp4HI	GCNGC	8 cut(s)	205, 263, 756, 879, 1575, 1661, 2778, 3513
FspBI	CTAG	9 cut(s)	786, 2282, 2504, 2630, 3108, 3182, 3827, 3909, 4101
GlaI	GCGC	5 cut(s)	126, 2337, 2339, 2346, 4123
GluI	GCNGC	8 cut(s)	205, 263, 756, 879, 1575, 1661, 2778, 3513
GsaI	CCCAGC	2 cut(s)	273, 1149
GsuI	CTGGAG	1 cut(s)	1245
HaeII	RGCGCY	1 cut(s)	2348
HaeIII	GGCC	6 cut(s)	18, 1803, 2353, 2516, 3574, 3924
HapII	CCGG	4 cut(s)	329, 498, 2813, 3249
HgaI	GACGC	1 cut(s)	91
HhaI	GCGC	5 cut(s)	127, 2338, 2340, 2347, 4124
Hin1I	GRCGYC	1 cut(s)	3018
Hin6I	GCGC	5 cut(s)	125, 2336, 2338, 2345, 4122
HinP1I	GCGC	5 cut(s)	125, 2336, 2338, 2345, 4122
HincII	GTYRAC	3 cut(s)	822, 4045, 4072
HindII	GTYRAC	3 cut(s)	822, 4045, 4072
HindIII	AAGCTT	1 cut(s)	845
HpaII	CCGG	4 cut(s)	329, 498, 2813, 3249
HpyCH4IV	ACGT	7 cut(s)	171, 1696, 2032, 3018, 3127, 3307, 3847
HpySE526I	ACGT	7 cut(s)	171, 1696, 2032, 3018, 3127, 3307, 3847
Hsp92I	GRCGYC	1 cut(s)	3018
HspAI	GCGC	5 cut(s)	125, 2336, 2338, 2345, 4122
Ksp22I	TGATCA	2 cut(s)	2083, 4167
LguI	GCTCTTC	2 cut(s)	660, 846
LmnI	GCTCC	8 cut(s)	506, 813, 1430, 1740, 2708, 3296, 3833, 4119
Lsp1109I	GCAGC	7 cut(s)	274, 767, 865, 1586, 1672, 2764, 3524
LweI	GCATC	6 cut(s)	1567, 1591, 1909, 2789, 3175, 3555
MaeI	CTAG	9 cut(s)	786, 2282, 2504, 2630, 3108, 3182, 3827, 3909, 4101
MaeII	ACGT	7 cut(s)	171, 1696, 2032, 3018, 3127, 3307, 3847
MaeIII	GTNAC	5 cut(s)	112, 371, 437, 621, 1132
MfeI	CAATTG	1 cut(s)	2433
MflI	RGATCY	7 cut(s)	332, 1845, 2155, 2386, 2539, 3973, 4142
MhlI	GDGCHC	4 cut(s)	810, 2118, 2713, 3853
MlsI	TGGCCA	1 cut(s)	2353
MluNI	TGGCCA	1 cut(s)	2353
MlyI	GAGTC	6 cut(s)	368, 376, 1313, 2171, 3773, 3878
MmeI	TCCRAC	9 cut(s)	1034, 1215, 1491, 2197, 2854, 3274, 3328, 3414, 3811
Mox20I	TGGCCA	1 cut(s)	2353
Mph1103I	ATGCAT	2 cut(s)	1560, 3745
MroXI	GAANNNNTTC	1 cut(s)	2973
MscI	TGGCCA	1 cut(s)	2353
MslI	CAYNNNNRTG	3 cut(s)	393, 444, 2148
Msp20I	TGGCCA	1 cut(s)	2353
MspA1I	CMGCKG	3 cut(s)	2777, 3508, 4063
MspI	CCGG	4 cut(s)	329, 498, 2813, 3249
MspR9I	CCNGG	8 cut(s)	330, 1262, 1529, 2342, 2411, 2513, 3576, 3843
MunI	CAATTG	1 cut(s)	2433
Mva1269I	GAATGC	1 cut(s)	1383
MvaI	CCWGG	7 cut(s)	1262, 1529, 2342, 2411, 2513, 3576, 3843
MvnI	CGCG	4 cut(s)	127, 946, 1770, 2338
NciI	CCSGG	1 cut(s)	330
NcoI	CCATGG	2 cut(s)	192, 4017
NlaIV	GGNNCC	4 cut(s)	451, 502, 1239, 1426
NmuCI	GTSAC	3 cut(s)	112, 371, 437
NruI	TCGCGA	1 cut(s)	1770
NsiI	ATGCAT	2 cut(s)	1560, 3745
NspI	RCATGY	4 cut(s)	965, 2291, 2584, 3075
NspV	TTCGAA	1 cut(s)	2821
OliI	CACNNNNGTG	1 cut(s)	393
PagI	TCATGA	1 cut(s)	2371
PalAI	GGCGCGCC	1 cut(s)	2336
PaqCI	CACCTGC	2 cut(s)	1252, 3151
PasI	CCCWGGG	1 cut(s)	1261
PauI	GCGCGC	1 cut(s)	2336
PciI	ACATGT	2 cut(s)	2287, 2580
PciSI	GCTCTTC	2 cut(s)	660, 846
PcsI	WCGNNNNNNNCGW	1 cut(s)	2056
PctI	GAATGC	1 cut(s)	1383
PdmI	GAANNNNTTC	1 cut(s)	2973
PflFI	GACNNNGTC	1 cut(s)	1583
PfoI	TCCNGGA	1 cut(s)	3841
PinAI	ACCGGT	1 cut(s)	3248
PkrI	GCNGC	8 cut(s)	206, 264, 757, 880, 1576, 1662, 2779, 3514
Ple19I	CGATCG	1 cut(s)	2050
PleI	GAGTC	6 cut(s)	368, 376, 1313, 2170, 3773, 3878
PmaCI	CACGTG	1 cut(s)	1697
PmlI	CACGTG	1 cut(s)	1697
PpsI	GAGTC	6 cut(s)	368, 376, 1313, 2170, 3773, 3878
Ppu21I	YACGTR	2 cut(s)	1697, 3308
PscI	ACATGT	2 cut(s)	2287, 2580
PshAI	GACNNNNGTC	1 cut(s)	3230
PshBI	ATTAAT	1 cut(s)	1955
PsiI	TTATAA	1 cut(s)	3932
Psp124BI	GAGCTC	1 cut(s)	810
Psp1406I	AACGTT	1 cut(s)	171
Psp6I	CCWGG	7 cut(s)	1260, 1527, 2340, 2409, 2511, 3574, 3841
PspCI	CACGTG	1 cut(s)	1697
PspFI	CCCAGC	2 cut(s)	269, 1145
PspGI	CCWGG	7 cut(s)	1260, 1527, 2340, 2409, 2511, 3574, 3841
PspN4I	GGNNCC	4 cut(s)	451, 502, 1239, 1426
PspPI	GGNCC	4 cut(s)	2427, 2468, 2515, 2725
PsrI	GAACNNNNNNTAC	2 cut(s)	1110, 1142
PstNI	CAGNNNCTG	2 cut(s)	662, 3425
PsuI	RGATCY	7 cut(s)	332, 1845, 2155, 2386, 2539, 3973, 4142
PsyI	GACNNNGTC	1 cut(s)	1583
PteI	GCGCGC	1 cut(s)	2336
PvuI	CGATCG	1 cut(s)	2050
PvuII	CAGCTG	2 cut(s)	2777, 3508
RruI	TCGCGA	1 cut(s)	1770
RseI	CAYNNNNRTG	3 cut(s)	393, 444, 2148
SacI	GAGCTC	1 cut(s)	810
SapI	GCTCTTC	2 cut(s)	660, 846
SatI	GCNGC	8 cut(s)	205, 263, 756, 879, 1575, 1661, 2778, 3513
Sau96I	GGNCC	4 cut(s)	2427, 2468, 2515, 2725
SchI	GAGTC	6 cut(s)	368, 376, 1313, 2171, 3773, 3878
ScrFI	CCNGG	8 cut(s)	330, 1262, 1529, 2342, 2411, 2513, 3576, 3843
SduI	GDGCHC	4 cut(s)	810, 2118, 2713, 3853
SfaNI	GCATC	6 cut(s)	1567, 1591, 1909, 2789, 3175, 3555
SfcI	CTRYAG	1 cut(s)	1341
SfuI	TTCGAA	1 cut(s)	2821
SgsI	GGCGCGCC	1 cut(s)	2336
SinI	GGWCC	3 cut(s)	2427, 2468, 2725
SmiMI	CAYNNNNRTG	3 cut(s)	393, 444, 2148
SmlI	CTYRAG	4 cut(s)	342, 2159, 2330, 3479
SmoI	CTYRAG	4 cut(s)	342, 2159, 2330, 3479
SsiI	CCGC	8 cut(s)	205, 248, 369, 946, 2430, 2641, 4061, 4084
SspI	AATATT	2 cut(s)	295, 3088
SspMI	CTAG	9 cut(s)	786, 2282, 2504, 2630, 3108, 3182, 3827, 3909, 4101
SstI	GAGCTC	1 cut(s)	810
StyD4I	CCNGG	8 cut(s)	328, 1260, 1527, 2340, 2409, 2511, 3574, 3841
StyI	CCWWGG	4 cut(s)	192, 1050, 3569, 4017
TaiI	ACGT	7 cut(s)	174, 1699, 2035, 3021, 3130, 3310, 3850
TaqI	TCGA	9 cut(s)	285, 552, 1452, 1561, 1837, 2821, 3952, 3978, 4128
TatI	WGTACW	5 cut(s)	673, 1250, 2221, 2278, 2484
TauI	GCSGC	1 cut(s)	207
TscAI	CASTG	8 cut(s)	247, 395, 1205, 1321, 2911, 2992, 3426, 4015
TseFI	GTSAC	3 cut(s)	112, 371, 437
TseI	GCWGC	7 cut(s)	262, 755, 878, 1574, 1660, 2777, 3512
Tsp45I	GTSAC	3 cut(s)	112, 371, 437
TspGWI	ACGGA	6 cut(s)	1742, 1795, 2048, 2753, 3477, 4089
TspRI	CASTG	8 cut(s)	247, 395, 1205, 1321, 2911, 2992, 3426, 4015
Tth111I	GACNNNGTC	1 cut(s)	1583
VpaK11BI	GGWCC	3 cut(s)	2427, 2468, 2725
VspI	ATTAAT	1 cut(s)	1955
XagI	CCTNNNNNAGG	2 cut(s)	2318, 3716
XbaI	TCTAGA	1 cut(s)	2503
XceI	RCATGY	4 cut(s)	965, 2291, 2584, 3075
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2941
XmiI	GTMKAC	3 cut(s)	722, 1793, 2098
XmnI	GAANNNNTTC	1 cut(s)	2973
XspI	CTAG	9 cut(s)	786, 2282, 2504, 2630, 3108, 3182, 3827, 3909, 4101
ZraI	GACGTC	1 cut(s)	3019
Zsp2I	ATGCAT	2 cut(s)	1560, 3745

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.