Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0005249

Physical Location & Seq

Genomic Coordinates Forward (+)

49503911 .. 49516424

Gene Structure 12514 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG329300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3969 bp

ATGAGCAACTCGGTGAGCCATAATATTCTCCAGCAGAATCTGCTTCAGAGCAAAATCAATTCTTCAGGCATTCCTGCCAACACATTGTTACAACCCAAGTGTATACATCCAGTGCCAGCGCAGCCGCGCAAGTCTTCTTCTATATCCAAGAAATTTTGTGGCAACAGTTTGCAGAAGCCGAAGCTGGCCATGGGGTCTCGTCCACCTGTTACATTTGTTCCTTGCGCTGTACTAACCACGGATCCGCATTCTGATCAGCTTGCCGGGAAATACAACCTTGATGAGAACATTGAATTGCAGGTTTATGTTGATGCTTCCTCTCCAGGGTCTGCCACTAGAGTAGATATTCAGGTCTCATATGGCGGTGACTCTTTGGTTCTTCATTGGGGTGGGATAAAAGATAGAAAAGAAAAATGGGTGCTTCCTTCTCGTCACCCAGATGGAACCAAAGAATATAAGAAGAGAGCTCTTAGAACTCCTTTTGCGAAATCTGGCTCCAATTCCGTACTTAAAATTGAGATTGATGATCCTGCAATACAAGCTATAGAGTTTCTCATAGTTGATGAAAGTCAGAACAGATGGTTTAAGAACAACGGCGGCAACTTTCATGTTAAATTACCTTTGAAAGAGAAGTTGATATCAAATGTTTCAGTTCCTGAAGATTTAGTGCAAATTCAAGCATATCTGAGGTGGGAGCGAAAGGGTAAACAGATGTACACACCAGAACAAGAGAAGGTGGAGTATGAAGCAGCCCGAACTGAGCTACTGGAGGAAGTAGCCAGGGGTGCTTCCATACAAGAGCTCCAAGCAAGGCTGACTAAGAAAACTGATTCTGGTAACAGTCAGGAACCATCTCATGCTGGTAACAAAGAAGTTTCCAAGGCCGCAAAGAAACATGAGAGTAAACAGGTTTTCAGAACTGGAGGAATTAAGCGGAAGCAACGAGACTTAATGAAGATTCTCAACAAACATGCTACTAAACCTGTAGATGAAGCTAAACTTGCACATGAAGAATCAACGAAACCAAAAGCCTTGAAAGCAGTTGAGCTATTTGCCAAGGCAAAAGAAGAACAAGATGGTGCTTCCGTCCTGAACAAAAAAATCTACAGGCTCGGTGATAAGGACCTGCTGGTACTTGTGACAAAGGTTGCTGACAAGATAAAGGTTCATCTGGCGACAGATATCAAAGAGCCAATTAACCTTCATTGGGCTCTATCTGTAAACAAAGCTGGAGAGTGGTTGGAACCACCTCCAAATGTACTGCCCCAGGGTTCAGTTTCTCTAAATGGGGCTGTTGAAACACAATTTGTATCTAGTTCTGTTGATTCTAATTATGAGGTTCAATCTTTAGAAATAGAAATGCAAGAGGAAAGTTTCAAAGGAATGCCATTTGTCCTTTGTTCTTCTGGAAACTGGATTAAGAACCAAGGCTCAGATTTCTACGCTGACTTTGGTGTTGCGGTCAAGAAAGTTCAGAAGGATGCTGGTGACGGGAAAGGTACTGCAAAGGATTTATTGGATAAAATAGCAGATCTGGAGAGTGAGGCGCAAAAATCCTTTATGCATCGGTTTAACATTGCAGCAGACTTGATAAATCAAGCAAAAGATGCTGGCGAATTAGGTCTTGCAGGTATTCTGGTGTGGATGAGGTTTATGGCTACGAGGCAGCTGATTTGGAATAAAAATTACAATGTCAAACCACGTGAGATTAGTAAAGCACAGGACAGGCTCACAGGCTTGCTCCAGAGTATTTACACAAGTCATCCACAGTATCGAGAACTTTTACGGATGATATTGTCTACTGTTGGACGTGGAGGAGAAGGGGATGTGGGACAGCGAATTCGAGATGAGATCCTTGTTATTCAGAGAAACTGTGAGTGTAAGGGCGGAATGATGGAGGAATGGCATCAAAAGTTGCATAATAACACTAGCCCAGATGATGTTGTTATATGTCAGGCGCTAATTGATTACATCAAAAGTGACTTTGATATTGGTGTTTACTGGAAAACTTTGAACAACAATGGAATTACAAAAGAACGTCTTCTAAGCTATGACCGTGCAATCCACAATGAACCAAGTTTCAGGAGAGATCAGAAAGAGCGTCTTCTGCATGATCTGGGGAACTACATGAGAACTTTAAAGGCAGTTCACTCAGGTGCAGATCTTGAGTCTGCCATCACAAATTGTTTGGGTTACAGTGCTCAGGGTCAAGGCTTCATGGTTGGAGTACAAATAAATCCTGTATCTGGCCTGCCCTCTGAAGTTCCAGGACTGCTGCAATTTGTAATGGAACATGTTGAGGACAAGAATGTGGAAGTCCTTATTGAGGGTTTGCTTGAGGCTCGTCAGGAGCTGTGGTCATTGCTCTCTAAGCCTAATGATCGCCTGAAGGATCTCTTATTTTTGGACATTGCCCTTGATTCTACTGTGAGAACCGCCATTGAGAGGGGATATGAAGAACTGAATAATGCTGGGCCAGAGAAAATTATGTACTTCATCTCAATGGTTCTAGAAAACCTGGCATTATCGTCAGATGATAATGAGGATCTCATTTACTGTTTGAAGGGATGGAATCATGCATTAAACATGCTAAAGAGTAATACCGATAACTGGGCATTATATGCAAAATCAATCCTAGACAGAACCCGCCTGGCACTTGCAAACAAGGCAGAGTCGTATCACAGTATTTTACAGCCATCAGCAGAGTACCTTGGATCAAAGCTTGGAGTGGACCAAGGGGCTTTGAGCATATTTACTGAAGAAGTTATCCGTGCTGGATCAGCTGCCTCTTTGTCCGCACTCGTTAATCGACTTGATCCAGTTCTTCGGAAGACTGCTAATTTGGGAAGTTGGCAGGTTATCAGCCCAGTTGAAGTGGTTGGATATGTTGTAGTTGTGGACGAGTTGCTCACTGTCCAGAATAAAGTATACGATAAGCCGACAATTTTGGTGGCAAGAAGTGTAAGAGGTGAGGAAGAAATTCCAGATGGCACAGTTGCTGTGCTAACACCTGATATGCCAGACGTCCTATCTCATGTTTCTGTCCGAGCAAGAAATAGCAAGGTTTGTTTTGCGACGTGTTTTGACTCCAATATTCTAGCTGACCTCCAAGCTTGTGAAGGGAAGTTATTGCGTGTAAAGCCAACTCCTGCTGATGTAGTTTATAGCGAGGTTAATGAGAGTGAGCTAGGAGATGCAAGTTCAACTAATACAAATGAAGACATCCCAGCTCTGACATTAGTGAAGAAACAGTTTACGGGTAGATATGCCATATCATCTGATGAGTTCACAAGTGAAATGGTTGGAGCTAAATCACGTAATATTTCTTATATAAAAGGAAAACTGCCCTCTTGGGTTGGAATTCCTACATCAGTTGCCCTACCATTTGGAGTATTTGAAAAAGTTCTTTCTGAAGATTCAAATAAGGCTGTTGCTGACAAGTTAGAAATTCTAAAGAAAAAGTTAAAAGAAGGAGATTTTGGTTCACTTGGGGAGATTCGAGAGACAGTTTTACAGTTGACAGCACCACCCCCGTTGGTGCAAGAGCTGAAGACTAAGATGCAAAGTTCAGGAATGCCTTGGCCTGGTGATGAGGGTGAACAACGATGGGAACAAGCATGGTTGGCTATAAAAAAGGTCTGGGCTTCAAAGTGGAATGAGAGAGCATACTTCAGCACAAGAAAAGTGAAATTAGACCATGATTATCTTTGCATGGCTGTCCTTGTTCAAGAAATAATAAGTGCCGACTATGCATTCGTTATTCACACAACTAACCCATCTTCAGGAGACTCGTCGGAGATATATGCCGAGGTTGTCAAAGGTCTTGGAGAAACCTTAGTTGGAGCTTATCCTGGACGTGCCTTAAGTTTTATTAGCAAGAAAAATGCTCTGGACTCTCCTCAGGTAATGCACTACAGTGCCAAAAGTGGCATAAGAGTAACAGACCATGTATGGATTGAATTGCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009628 GO:0009631 GO:0009941 GO:0009987 GO:0016052 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016781 GO:0031967 GO:0031975 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050521 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPDK_N

Protein Analysis

1322

Amino Acids

147.0

Weight (kDa)

6.52

Isoelectric Point (pI)

41.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ig_N_CWD1	PF23166	89 - 206	6e-41	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
DUF7067	PF23229	220 - 275	3.8e-28	Alpha-glucan water dikinase, DUF7067
Ig_N_CWD1	PF23166	365 - 483	2.8e-27	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
GWD1_pHisD	PF22973	950 - 1062	2.5e-53	Alpha-glucan water dikinase, phosphohistidine-like domain
PPDK_N	PF01326	1205 - 1281	2.2e-12	Pyruvate phosphate dikinase, AMP/ATP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10760 AT1G10760 AT1G10760
fragaria_vesca	FvH4_4g25800 FvH4_4g25800 FvH4_4g25800 FvH4_4g25800
malus_domestica	MD13G1096800.v1.1 MD16G1097800.v1.1
prunus_persica	Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1
pyrus_communis	pycom13g08450 pycom16g08330
rosa_chinensis	RchiOBHm_Chr4g0433251
rosa_laevigata	RLG00000006771
rosa_multiflora	Rmu_co8055492.1_g000001 Rmu_sc0010643.1_g000001
rosa_roxburghii	Rroxscaffold_5G00374810
rosa_rugosa	Rorug04G0267200 Rorug04G0267300 Rorug04G0267400 Rorug04G0267400
rosa_samantha	Rh4AG321900 Rh4BG329300 Rh4CG344600
rosa_wichuraiana	Rw4G027860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	3027
Acc36I	ACCTGC	4 cut(s)	289, 1134, 1619, 2845
AccI	GTMKAC	3 cut(s)	103, 1799, 2928
AccII	CGCG	1 cut(s)	127
AclWI	GGATC	9 cut(s)	236, 249, 521, 1846, 2400, 2553, 2722, 2785, 2810
AcoI	YGGCCR	1 cut(s)	186
AcsI	RAATTY	6 cut(s)	152, 672, 1839, 2979, 3360, 3447
AcvI	CACGTG	1 cut(s)	1703
AcyI	GRCGYC	1 cut(s)	3024
AfaI	GTAC	9 cut(s)	231, 507, 716, 1134, 1260, 1501, 2229, 2492, 2708
AfiI	CCNNNNNNNGG	8 cut(s)	324, 1207, 2246, 2326, 2445, 2610, 3584, 3782
AflII	CTTAAG	1 cut(s)	3862
AflIII	ACRYGT	2 cut(s)	2293, 3077
AjiI	CACGTC	3 cut(s)	1811, 3078, 3857
AjnI	CCWGG	8 cut(s)	322, 779, 1266, 2266, 2517, 2649, 3583, 3850
AjuI	GAANNNNNNNTTGG	4 cut(s)	3462, 3494, 3822, 3854
AleI	CACNNNNGTG	2 cut(s)	2154, 3915
Alw21I	GWGCWC	3 cut(s)	469, 804, 2203
Alw26I	GTCTC	5 cut(s)	201, 358, 939, 3497, 3780
AlwI	GGATC	9 cut(s)	236, 249, 521, 1846, 2400, 2553, 2722, 2785, 2810
AlwNI	CAGNNNCTG	5 cut(s)	40, 329, 656, 2353, 2999
AoxI	GGCC	5 cut(s)	186, 882, 2248, 2474, 3581
ApeKI	GCWGC	6 cut(s)	121, 749, 1580, 1666, 2275, 2783
ApoI	RAATTY	6 cut(s)	152, 672, 1839, 2979, 3360, 3447
ArsI	GACNNNNNNTTYG	6 cut(s)	1824, 1856, 2824, 2856, 3737, 3769
Asp700I	GAANNNNTTC	3 cut(s)	2040, 2979, 3402
AspLEI	GCGC	5 cut(s)	121, 129, 227, 1549, 1960
AspS9I	GGNCC	3 cut(s)	1123, 2474, 2731
AsuC2I	CCSGG	1 cut(s)	265
AsuHPI	GGTGA	8 cut(s)	25, 377, 425, 1127, 1499, 2981, 3599, 3608
AvaII	GGWCC	2 cut(s)	1123, 2731
AxyI	CCTNAGG	1 cut(s)	3900
BalI	TGGCCA	1 cut(s)	188
BamHI	GGATCC	1 cut(s)	241
BanII	GRGCYC	3 cut(s)	469, 804, 1213
BbrPI	CACGTG	1 cut(s)	1703
BbsI	GAAGAC	6 cut(s)	126, 2033, 2096, 2837, 3225, 3557
Bbv12I	GWGCWC	3 cut(s)	469, 804, 2203
BbvI	GCAGC	6 cut(s)	133, 761, 1592, 1678, 2262, 2770
BceAI	ACGGC	1 cut(s)	610
BciT130I	CCWGG	8 cut(s)	324, 781, 1268, 2268, 2519, 2651, 3585, 3852
BclI	TGATCA	1 cut(s)	253
BcnI	CCSGG	1 cut(s)	265
BcoDI	GTCTC	5 cut(s)	201, 358, 939, 3497, 3780
BfaI	CTAG	7 cut(s)	336, 1314, 1929, 2510, 2636, 3098, 3188
BfmI	CTRYAG	4 cut(s)	543, 984, 1105, 3913
BfoI	RGCGCY	1 cut(s)	1961
BfrI	CTTAAG	1 cut(s)	3862
BfuAI	ACCTGC	4 cut(s)	289, 1134, 1619, 2845
BglII	AGATCT	2 cut(s)	1531, 2161
BisI	GCNGC	9 cut(s)	122, 125, 598, 750, 885, 1581, 1667, 2276, 2784
BlsI	GCNGC	9 cut(s)	123, 126, 599, 751, 886, 1582, 1668, 2277, 2785
Bme1390I	CCNGG	9 cut(s)	265, 324, 781, 1268, 2268, 2519, 2651, 3585, 3852
Bme18I	GGWCC	2 cut(s)	1123, 2731
BmgBI	CACGTC	3 cut(s)	1811, 3078, 3857
BmgT120I	GGNCC	3 cut(s)	1123, 2474, 2731
BmiI	GGNNCC	5 cut(s)	243, 445, 496, 849, 1245
BmrFI	CCNGG	9 cut(s)	265, 324, 781, 1268, 2268, 2519, 2651, 3585, 3852
BmrI	ACTGGG	2 cut(s)	2620, 2861
BmsI	GCATC	7 cut(s)	301, 1471, 1573, 1597, 1915, 3184, 3549
BmuI	ACTGGG	2 cut(s)	2620, 2861
BpiI	GAAGAC	6 cut(s)	126, 2033, 2096, 2837, 3225, 3557
BpmI	CTGGAG	7 cut(s)	14, 306, 788, 942, 1251, 1556, 1727
Bpu10I	CCTNAGC	1 cut(s)	2202
BpuEI	CTTGAG	2 cut(s)	2186, 2357
BpuMI	CCSGG	1 cut(s)	265
BsaAI	YACGTR	2 cut(s)	1703, 3317
BsaHI	GRCGYC	1 cut(s)	3024
BsaI	GGTCTC	2 cut(s)	201, 358
BsaXI	ACNNNNNCTCC	4 cut(s)	786, 816, 2342, 2372
Bsc4I	CCNNNNNNNGG	8 cut(s)	324, 1207, 2246, 2326, 2445, 2610, 3584, 3782
Bse1I	ACTGG	8 cut(s)	110, 771, 925, 1418, 2006, 2615, 2819, 2867
Bse21I	CCTNAGG	1 cut(s)	3900
Bse3DI	GCAATG	3 cut(s)	1575, 2360, 2409
BseBI	CCWGG	8 cut(s)	324, 781, 1268, 2268, 2519, 2651, 3585, 3852
BseGI	GGATG	8 cut(s)	106, 1486, 1650, 1762, 1794, 1831, 2573, 3222
BseLI	CCNNNNNNNGG	8 cut(s)	324, 1207, 2246, 2326, 2445, 2610, 3584, 3782
BseMI	GCAATG	3 cut(s)	1575, 2360, 2409
BseMII	CTCAG	6 cut(s)	677, 750, 1446, 2166, 2216, 3914
BseNI	ACTGG	8 cut(s)	110, 771, 925, 1418, 2006, 2615, 2819, 2867
BseRI	GAGGAG	2 cut(s)	1830, 3888
BseXI	GCAGC	6 cut(s)	133, 761, 1592, 1678, 2262, 2770
BseYI	CCCAGC	2 cut(s)	2471, 3226
BsgI	GTGCAG	1 cut(s)	2178
Bsh1236I	CGCG	1 cut(s)	127
BshFI	GGCC	5 cut(s)	188, 884, 2250, 2476, 3583
BsiHKAI	GWGCWC	3 cut(s)	469, 804, 2203
BsiSI	CCGG	1 cut(s)	264
BslFI	GGGAC	1 cut(s)	1845
BslI	CCNNNNNNNGG	8 cut(s)	324, 1207, 2246, 2326, 2445, 2610, 3584, 3782
BsmAI	GTCTC	5 cut(s)	201, 358, 939, 3497, 3780
BsmFI	GGGAC	1 cut(s)	1845
BsmI	GAATGC	5 cut(s)	69, 247, 1389, 3579, 3752
BsnI	GGCC	5 cut(s)	188, 884, 2250, 2476, 3583
Bso31I	GGTCTC	2 cut(s)	201, 358
Bsp1286I	GDGCHC	4 cut(s)	469, 804, 1213, 2203
Bsp1407I	TGTACA	1 cut(s)	714
Bsp19I	CCATGG	1 cut(s)	189
BspANI	GGCC	5 cut(s)	188, 884, 2250, 2476, 3583
BspCNI	CTCAG	6 cut(s)	678, 751, 1445, 2165, 2215, 3913
BspFNI	CGCG	1 cut(s)	127
BspLI	GGNNCC	5 cut(s)	243, 445, 496, 849, 1245
BspMI	ACCTGC	4 cut(s)	289, 1134, 1619, 2845
BspPI	GGATC	9 cut(s)	236, 249, 521, 1846, 2400, 2553, 2722, 2785, 2810
BspTI	CTTAAG	1 cut(s)	3862
BspTNI	GGTCTC	2 cut(s)	201, 358
BsrDI	GCAATG	3 cut(s)	1575, 2360, 2409
BsrGI	TGTACA	1 cut(s)	714
BsrI	ACTGG	8 cut(s)	110, 771, 925, 1418, 2006, 2615, 2819, 2867
BssNAI	GTATAC	2 cut(s)	104, 2929
BssNI	GRCGYC	1 cut(s)	3024
BssT1I	CCWWGG	7 cut(s)	189, 879, 1056, 1426, 2710, 2734, 3578
Bst1107I	GTATAC	2 cut(s)	104, 2929
Bst2UI	CCWGG	8 cut(s)	324, 781, 1268, 2268, 2519, 2651, 3585, 3852
Bst6I	CTCTTC	1 cut(s)	455
BstACI	GRCGYC	1 cut(s)	3024
BstAFI	CTTAAG	1 cut(s)	3862
BstAPI	GCANNNNNTGC	3 cut(s)	40, 1607, 2621
BstAUI	TGTACA	1 cut(s)	714
BstBAI	YACGTR	2 cut(s)	1703, 3317
BstC8I	GCNNGC	6 cut(s)	117, 186, 261, 1612, 1739, 2252
BstDSI	CCRYGG	2 cut(s)	189, 237
BstENI	CCTNNNNNAGG	1 cut(s)	2324
BstF5I	GGATG	8 cut(s)	106, 1486, 1650, 1762, 1794, 1831, 2573, 3222
BstFNI	CGCG	1 cut(s)	127
BstH2I	RGCGCY	1 cut(s)	1961
BstHHI	GCGC	5 cut(s)	121, 129, 227, 1549, 1960
BstMAI	GTCTC	5 cut(s)	201, 358, 939, 3497, 3780
BstNI	CCWGG	8 cut(s)	324, 781, 1268, 2268, 2519, 2651, 3585, 3852
BstNSI	RCATGY	3 cut(s)	974, 2297, 2590
BstSCI	CCNGG	9 cut(s)	263, 322, 779, 1266, 2266, 2517, 2649, 3583, 3850
BstSFI	CTRYAG	4 cut(s)	543, 984, 1105, 3913
BstUI	CGCG	1 cut(s)	127
BstV1I	GCAGC	6 cut(s)	133, 761, 1592, 1678, 2262, 2770
BstV2I	GAAGAC	6 cut(s)	126, 2033, 2096, 2837, 3225, 3557
BstX2I	RGATCY	6 cut(s)	241, 1531, 1851, 2161, 2392, 2545
BstYI	RGATCY	6 cut(s)	241, 1531, 1851, 2161, 2392, 2545
BstZ17I	GTATAC	2 cut(s)	104, 2929
Bsu36I	CCTNAGG	1 cut(s)	3900
BsuRI	GGCC	5 cut(s)	188, 884, 2250, 2476, 3583
BtgI	CCRYGG	2 cut(s)	189, 237
BtrI	CACGTC	3 cut(s)	1811, 3078, 3857
BtsCI	GGATG	8 cut(s)	106, 1486, 1650, 1762, 1794, 1831, 2573, 3222
BtsIMutI	CAGTG	4 cut(s)	117, 2203, 2910, 3922
BveI	ACCTGC	4 cut(s)	289, 1134, 1619, 2845
Cac8I	GCNNGC	6 cut(s)	117, 186, 261, 1612, 1739, 2252
CaiI	CAGNNNCTG	5 cut(s)	40, 329, 656, 2353, 2999
CfoI	GCGC	5 cut(s)	121, 129, 227, 1549, 1960
Cfr13I	GGNCC	3 cut(s)	1123, 2474, 2731
CseI	GACGC	1 cut(s)	2090
Csp6I	GTAC	9 cut(s)	230, 506, 715, 1133, 1259, 1500, 2228, 2491, 2707
CspCI	CAANNNNNGTGG	4 cut(s)	2931, 2966, 3516, 3551
CviQI	GTAC	9 cut(s)	230, 506, 715, 1133, 1259, 1500, 2228, 2491, 2707
DraI	TTTAAA	1 cut(s)	2139
EaeI	YGGCCR	1 cut(s)	186
Eam1104I	CTCTTC	1 cut(s)	455
EarI	CTCTTC	1 cut(s)	455
EciI	GGCGGA	1 cut(s)	1902
Ecl136II	GAGCTC	2 cut(s)	467, 802
Eco130I	CCWWGG	7 cut(s)	189, 879, 1056, 1426, 2710, 2734, 3578
Eco24I	GRGCYC	3 cut(s)	469, 804, 1213
Eco31I	GGTCTC	2 cut(s)	201, 358
Eco32I	GATATC	2 cut(s)	639, 1183
Eco47I	GGWCC	2 cut(s)	1123, 2731
Eco53kI	GAGCTC	2 cut(s)	467, 802
Eco72I	CACGTG	1 cut(s)	1703
Eco81I	CCTNAGG	1 cut(s)	3900
EcoICRI	GAGCTC	2 cut(s)	467, 802
EcoNI	CCTNNNNNAGG	1 cut(s)	2324
EcoO109I	RGGNCCY	1 cut(s)	1123
EcoRI	GAATTC	2 cut(s)	1839, 3360
EcoRII	CCWGG	8 cut(s)	322, 779, 1266, 2266, 2517, 2649, 3583, 3850
EcoRV	GATATC	2 cut(s)	639, 1183
EcoT14I	CCWWGG	7 cut(s)	189, 879, 1056, 1426, 2710, 2734, 3578
EcoT22I	ATGCAT	3 cut(s)	1566, 2581, 3754
EcoT38I	GRGCYC	3 cut(s)	469, 804, 1213
ErhI	CCWWGG	7 cut(s)	189, 879, 1056, 1426, 2710, 2734, 3578
FalI	AAGNNNNNCTT	8 cut(s)	984, 1016, 2025, 2057, 2088, 2120, 2381, 2413
FaqI	GGGAC	1 cut(s)	1845
FauI	CCCGC	1 cut(s)	2654
FauNDI	CATATG	1 cut(s)	358
FbaI	TGATCA	1 cut(s)	253
FblI	GTMKAC	3 cut(s)	103, 1799, 2928
Fnu4HI	GCNGC	9 cut(s)	122, 125, 598, 750, 885, 1581, 1667, 2276, 2784
FokI	GGATG	8 cut(s)	93, 1493, 1657, 1749, 1801, 1838, 2580, 3209
FriOI	GRGCYC	3 cut(s)	469, 804, 1213
Fsp4HI	GCNGC	9 cut(s)	122, 125, 598, 750, 885, 1581, 1667, 2276, 2784
FspBI	CTAG	7 cut(s)	336, 1314, 1929, 2510, 2636, 3098, 3188
GlaI	GCGC	5 cut(s)	120, 128, 226, 1548, 1959
GluI	GCNGC	9 cut(s)	122, 125, 598, 750, 885, 1581, 1667, 2276, 2784
GsaI	CCCAGC	2 cut(s)	2475, 3230
GsuI	CTGGAG	7 cut(s)	14, 306, 788, 942, 1251, 1556, 1727
HaeII	RGCGCY	1 cut(s)	1961
HaeIII	GGCC	5 cut(s)	188, 884, 2250, 2476, 3583
HapII	CCGG	1 cut(s)	264
HgaI	GACGC	1 cut(s)	2090
HhaI	GCGC	5 cut(s)	121, 129, 227, 1549, 1960
Hin1I	GRCGYC	1 cut(s)	3024
Hin6I	GCGC	5 cut(s)	119, 127, 225, 1547, 1958
HinP1I	GCGC	5 cut(s)	119, 127, 225, 1547, 1958
HincII	GTYRAC	1 cut(s)	3519
HindII	GTYRAC	1 cut(s)	3519
HindIII	AAGCTT	2 cut(s)	2720, 3111
HpaII	CCGG	1 cut(s)	264
HphI	GGTGA	8 cut(s)	25, 377, 425, 1127, 1499, 2981, 3599, 3608
Hpy99I	CGWCG	2 cut(s)	3079, 3796
HpyAV	CCTTC	9 cut(s)	435, 727, 1211, 1471, 1814, 2383, 2557, 3113, 3464
HpyCH4IV	ACGT	7 cut(s)	1702, 1810, 2038, 3024, 3077, 3316, 3856
HpySE526I	ACGT	7 cut(s)	1702, 1810, 2038, 3024, 3077, 3316, 3856
Hsp92I	GRCGYC	1 cut(s)	3024
HspAI	GCGC	5 cut(s)	119, 127, 225, 1547, 1958
Ksp22I	TGATCA	1 cut(s)	253
LmnI	GCTCC	7 cut(s)	500, 694, 807, 1746, 2350, 3305, 3842
Lsp1109I	GCAGC	6 cut(s)	133, 761, 1592, 1678, 2262, 2770
LweI	GCATC	7 cut(s)	301, 1471, 1573, 1597, 1915, 3184, 3549
MaeI	CTAG	7 cut(s)	336, 1314, 1929, 2510, 2636, 3098, 3188
MaeII	ACGT	7 cut(s)	1702, 1810, 2038, 3024, 3077, 3316, 3856
MflI	RGATCY	6 cut(s)	241, 1531, 1851, 2161, 2392, 2545
MhlI	GDGCHC	4 cut(s)	469, 804, 1213, 2203
MlsI	TGGCCA	1 cut(s)	188
MluNI	TGGCCA	1 cut(s)	188
MlyI	GAGTC	6 cut(s)	362, 2177, 2681, 3080, 3782, 3887
MmeI	TCCRAC	8 cut(s)	1221, 1786, 2203, 2860, 3283, 3337, 3774, 3820
Mox20I	TGGCCA	1 cut(s)	188
Mph1103I	ATGCAT	3 cut(s)	1566, 2581, 3754
MroXI	GAANNNNTTC	3 cut(s)	2040, 2979, 3402
MscI	TGGCCA	1 cut(s)	188
MslI	CAYNNNNRTG	5 cut(s)	387, 438, 2154, 2501, 3915
Msp20I	TGGCCA	1 cut(s)	188
MspA1I	CMGCKG	2 cut(s)	1669, 2783
MspCI	CTTAAG	1 cut(s)	3862
MspI	CCGG	1 cut(s)	264
MspR9I	CCNGG	9 cut(s)	265, 324, 781, 1268, 2268, 2519, 2651, 3585, 3852
Mva1269I	GAATGC	5 cut(s)	69, 247, 1389, 3579, 3752
MvaI	CCWGG	8 cut(s)	324, 781, 1268, 2268, 2519, 2651, 3585, 3852
MvnI	CGCG	1 cut(s)	127
NciI	CCSGG	1 cut(s)	265
NcoI	CCATGG	1 cut(s)	189
NdeI	CATATG	1 cut(s)	358
NlaIV	GGNNCC	5 cut(s)	243, 445, 496, 849, 1245
NmeAIII	GCCGAG	1 cut(s)	3832
NmuCI	GTSAC	5 cut(s)	365, 431, 1138, 1487, 1979
NsiI	ATGCAT	3 cut(s)	1566, 2581, 3754
NspI	RCATGY	3 cut(s)	974, 2297, 2590
OliI	CACNNNNGTG	2 cut(s)	2154, 3915
PasI	CCCWGGG	1 cut(s)	1267
PciI	ACATGT	1 cut(s)	2293
PctI	GAATGC	5 cut(s)	69, 247, 1389, 3579, 3752
PdmI	GAANNNNTTC	3 cut(s)	2040, 2979, 3402
PfeI	GAWTC	9 cut(s)	37, 830, 958, 1013, 1325, 2420, 2572, 3416, 3496
PfoI	TCCNGGA	2 cut(s)	2266, 3850
PkrI	GCNGC	9 cut(s)	123, 126, 599, 751, 886, 1582, 1668, 2277, 2785
PleI	GAGTC	6 cut(s)	362, 2176, 2680, 3080, 3782, 3887
PmaCI	CACGTG	1 cut(s)	1703
PmlI	CACGTG	1 cut(s)	1703
PpsI	GAGTC	6 cut(s)	362, 2176, 2680, 3080, 3782, 3887
Ppu21I	YACGTR	2 cut(s)	1703, 3317
PpuMI	RGGWCCY	1 cut(s)	1123
PscI	ACATGT	1 cut(s)	2293
Psp124BI	GAGCTC	2 cut(s)	469, 804
Psp5II	RGGWCCY	1 cut(s)	1123
Psp6I	CCWGG	8 cut(s)	322, 779, 1266, 2266, 2517, 2649, 3583, 3850
PspCI	CACGTG	1 cut(s)	1703
PspFI	CCCAGC	2 cut(s)	2471, 3226
PspGI	CCWGG	8 cut(s)	322, 779, 1266, 2266, 2517, 2649, 3583, 3850
PspN4I	GGNNCC	5 cut(s)	243, 445, 496, 849, 1245
PspPI	GGNCC	3 cut(s)	1123, 2474, 2731
PspPPI	RGGWCCY	1 cut(s)	1123
PstNI	CAGNNNCTG	5 cut(s)	40, 329, 656, 2353, 2999
PsuI	RGATCY	6 cut(s)	241, 1531, 1851, 2161, 2392, 2545
PvuII	CAGCTG	2 cut(s)	1669, 2783
RsaI	GTAC	9 cut(s)	231, 507, 716, 1134, 1260, 1501, 2229, 2492, 2708
RsaNI	GTAC	9 cut(s)	230, 506, 715, 1133, 1259, 1500, 2228, 2491, 2707
RseI	CAYNNNNRTG	5 cut(s)	387, 438, 2154, 2501, 3915
SacI	GAGCTC	2 cut(s)	469, 804
SatI	GCNGC	9 cut(s)	122, 125, 598, 750, 885, 1581, 1667, 2276, 2784
Sau96I	GGNCC	3 cut(s)	1123, 2474, 2731
SchI	GAGTC	6 cut(s)	362, 2177, 2681, 3080, 3782, 3887
ScrFI	CCNGG	9 cut(s)	265, 324, 781, 1268, 2268, 2519, 2651, 3585, 3852
SduI	GDGCHC	4 cut(s)	469, 804, 1213, 2203
SfaNI	GCATC	7 cut(s)	301, 1471, 1573, 1597, 1915, 3184, 3549
SfcI	CTRYAG	4 cut(s)	543, 984, 1105, 3913
SinI	GGWCC	2 cut(s)	1123, 2731
SmiMI	CAYNNNNRTG	5 cut(s)	387, 438, 2154, 2501, 3915
SmlI	CTYRAG	3 cut(s)	2165, 2336, 3862
SmoI	CTYRAG	3 cut(s)	2165, 2336, 3862
SspI	AATATT	3 cut(s)	25, 3094, 3322
SspMI	CTAG	7 cut(s)	336, 1314, 1929, 2510, 2636, 3098, 3188
SstI	GAGCTC	2 cut(s)	469, 804
StyD4I	CCNGG	9 cut(s)	263, 322, 779, 1266, 2266, 2517, 2649, 3583, 3850
StyI	CCWWGG	7 cut(s)	189, 879, 1056, 1426, 2710, 2734, 3578
TaiI	ACGT	7 cut(s)	1705, 1813, 2041, 3027, 3080, 3319, 3859
TaqI	TCGA	4 cut(s)	1774, 1843, 2809, 3499
TatI	WGTACW	5 cut(s)	229, 714, 1258, 2227, 2490
TauI	GCSGC	3 cut(s)	127, 600, 887
TfiI	GAWTC	9 cut(s)	37, 830, 958, 1013, 1325, 2420, 2572, 3416, 3496
TscAI	CASTG	4 cut(s)	117, 2203, 2917, 3922
TseFI	GTSAC	5 cut(s)	365, 431, 1138, 1487, 1979
TseI	GCWGC	6 cut(s)	121, 749, 1580, 1666, 2275, 2783
Tsp45I	GTSAC	5 cut(s)	365, 431, 1138, 1487, 1979
TspGWI	ACGGA	5 cut(s)	254, 493, 1075, 1801, 2759
TspRI	CASTG	4 cut(s)	117, 2203, 2917, 3922
Vha464I	CTTAAG	1 cut(s)	3862
VpaK11BI	GGWCC	2 cut(s)	1123, 2731
XagI	CCTNNNNNAGG	1 cut(s)	2324
XapI	RAATTY	6 cut(s)	152, 672, 1839, 2979, 3360, 3447
XbaI	TCTAGA	1 cut(s)	2509
XceI	RCATGY	3 cut(s)	974, 2297, 2590
XmiI	GTMKAC	3 cut(s)	103, 1799, 2928
XmnI	GAANNNNTTC	3 cut(s)	2040, 2979, 3402
XspI	CTAG	7 cut(s)	336, 1314, 1929, 2510, 2636, 3098, 3188
ZraI	GACGTC	1 cut(s)	3025
Zsp2I	ATGCAT	3 cut(s)	1566, 2581, 3754

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.