Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0005249

Physical Location & Seq

Genomic Coordinates Forward (+)

55758972 .. 55770464

Gene Structure 11493 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00374810.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4221 bp

ATGAGCAACTCGGTGAGCCATAATTTTCTCCAGCAGAATCTGCTTCAGAGCAAAATCAATTCTTCAGGCATTCCTGCCAACACATTGTTACAACCCAAGTGTATACATCAAGTGCCAGCGCAGCCGCGCAAGTCTTCTTCTATAGCCAAGAAATTTTGTGGCAACAGTTTGCAGAAGCCGAAGCTGGCCATGGGGTCTCGTCGGCCTGTTACATTTGTTCCTTGTGCTGTACTAACCACGGATCCGCATTCTGATCTTGCCGGGAAATACAACCTTGATGGTAACATTGAATTGCAGGTTTATGTTGATGCTTCCTCTCCAGGGACTGCCACTAAAGTAGATATTCAGGTCTCATATGGCGGTGATTCTTTGGTTCTTCATTGGGGTGGGATAGAAGATAGAAAAGAAAAATGGGTGCTTCCTTCTCGTCGCCCAGATGGAACCAAAGAATATAAGAAGAGAGCTCTTAGAACTCCTTTTGCGAAATCTGGCTCCAATTCCGTACTTAAGATTGAGATTGATGATCCTGCAATACAAGCTATAGAGTTTCTCATAGTTGATGAAAGTCAGAACAGATGGTTTAAGAACAATGGCGGCAACTTTCATGTTAAATTACCTTTGAAAGAGAAGTTGATATCAAATGTTTCAGTTCCTGAAGATTTAGTGCAAATTCAAGCATATCTGAGGTGGGAGCGAAAGGGTAAAAAGATGTACACACCAGAGCAAGAGAAGGTGGAGTATGAAGCAGCCCGAACTGAGCTATTGGAGGAAGTAGCCAGGGGTGCTTCCATGCAAGAGCTCCGAGCAAGGCTGACTAAGAAAACTGATGCTAGTAACAGTCAGGAACCATCTCATGGTGGTAACAAAGAAGTTTCCAAGGCCGCAAAGAAACATGAGAGTAAACAGGTTTTCAGAACTGGAAGAATTAAGCGGAAGCAACGAGACTTAATGAAGATTCTCAACAAACATGCTACTAAACCAGTAGATGAAGCTAAACTTGCACATGAAGAATCAACGAAACCAAAAGCCTTGAAAGCAGTTGAGCTATTTGCCAAGGCAAAAGAAGAACAAGATGGTGCTTCCATCCTGAACAAAAAAATCTACAGGCTCGGTGATAACGACCTGCTGGTACTTGTGACAAAGGTTGCTGACAAGATAAAGGTTCATCTGGCAACAGATATCAAAGAGCCAATTAACGTTCATTGGGCTCTATCTGTAAACAAAGCTGGAGAGTGGTTGGAACCACCTCCAAATGTACTGCCCCAGGGTTCAGTTTCTCTAAATGGGGCTGTTGAAACACAATTTGTATCTAGTTCTGTTGATTCTATTTATGAGGTTCAATCTTTAGAAATAGAAATGCAAGAGGAAAGTTTCAAGGGCATGCCATTTGTCCTATGTTCTTCTGGAAACTGGATTAAAAACCAAGGCTCAGATTTCTACGCTGACTTTGGTGTTGCGGTCAAGAAAGTTCAGAAGGATGCTGGTGATGGGAAAGGTACTGCAAAGGATTTATTGGATAAAATAGCAGATCTGGAGAGTGAGGCGCAAAAATCCTTTATGCATCGGTTTAACATTGCAGCAGACTTGATAAATCAAGCAAAAGATGCTGGCGAATTAGGTCTTGCAGGTATTCTGGTGTGGATGAGGTTTATGGCAACGAGGCAGCTGATTTGGAATAAAAATTACAATGTCAAACCACGTGAGATTAGTAAAGCACAGGACAGGCTCACAGGCTTGCTCCAGAATATTTACACAAGTCATCCACAGTATCGAGAGCTTCTACGGATGATATTGTCTACTGTTGGACGTGGAGGAGAAGGGGATGTGGGACAGCGAATTCGAGATGAGATCCTTGTTATCCAGAGAAACTGTGATTGTAAGGGCGGAATGATGGAGGAATGGCATCAAAAGTTGCATAATAACACTAGCCCAGATGATGTTGTTATATGTCAGGCACTAATTGATTACATCAAAAGTGACTTTGATATTGGTGTTTACTGGAAAACTTTGAACAAGAATGGAATTACAAAAGAACGTCTTCTAAGCTATGACCGTGCAATCCACAATGAACCAAGTTTCAGGGCAGATCAGAAAGAGCGTCTTCTGCATGATCTGGGGAACTACATGAGAACTTTAAAGGCAGTTCACTCAGGTGCAGATCTTGAGTCTGCCATCACAAATTGTTTGGGTTACAGTGCTCAGGGTGAAGGCTTCATGGTTGGAGTACAAATAAATCCTGTATCTGGCCTACCCTCTGAAGTTCCAGGACTGCTGCAATTTGTAATGGAACATGTTGAGGACAAGAATGTGGAAGTCCTTATTGAGGGTTTGCTTGAGGCTCGTCAGGAGCTGTGGCCATTGCTCTCTAGGCCTAATGATCGCCTGAAGGATCTCTTATTTTTGGACATTGCCCTTGATTCTACTGTGAGAACCGCCATTGAGAGGGGATATGAAGAACTGAATAATGCTGGGCCGGAGAAAATTATGTACTTCATCTCAATGGTTCTAGAAAACCTGGCATTATCATCAGATGATAATGAGGATCTCATCTACTGTTTGAAGGGATGGAATCATGCGTTAAACATGTTAAAGAGTAATACCGATAACTGGGCACTATATGCAAAATCAATTCTAGACAGAACCCGCCTGGCGCTTGCAAACAAGGCAGAGTCGTATCACAGTATTTTACAGCCATCAGCAGAGTACCTTGGATCAAAGCTTGGAGTGGACCAATGGGCTTTGAGCATATTTACTGAAGAAGTTATCCGTGCTGGATCAGCTGCCTCTTTGTCCGCACTCGTTAATCGACTTGATCCAGTTCTTCGGAAGACTGCTAATTTGGGAAGTTGGCAGGTTATCAGCCCAGTTGAAGTGGTTGGATATGTTGTAGTTGTGGACGAGTTGCTCACTGTCCAGAATAAAGTATATGAGAAGCCGACTATTTTGGTGGCAAGAAGTGTGAGAGGTGAGGAAGAAATTCCAGATGGCACAGTTGCTGTGCTAACACCGGATATGCCAGACGTCCTATCTCATGTTTCTGTCCGAGCAAGAAATAGCAAGGTTTGTTTTGCGACATGTTTTGACTCCAATATTCTAGCTGACCTCCAAGCTTGTAAAGGGAAGTTATTGCGTGTAAAGCCAACTTCTGCTGATGTAGTTTATAGCGAGGTTAATGAGAGTGAGCTAGGAGATGGAACTTCAACTGATTCAAATGAAGACATCCCAGCTCTGACATTAGTGAAGAAACAGTTTACAGGTAGATATGCCATATCATCTGATGAGTTCACAAGTGAAATGGTTGGAGCTAAATCACGTAATATTTATTATATAAAAGGAAAACTGCCCTCTTGGGTTGGAATTCCTACATCAGTTGCCCTACCATTTGGAGTATTTGAAAAAGTTCTTTCTGAAGATTCAAATAAGGCTGTTGCTGACAAGTTAGAAATTCTAAAGAAAAAGTTAAAAGAAGGAGATTTTGGTTCACTTGGGGAGATTCGAGAGACAGTTTTACAGTTGACAGCACCACCCCCGTTGGTGCAAGAGCTGAAGACTAAGATGCAAAGTTCAGGAATGCCTTGGCCTGGTGATGAGGGTGAACAACGATGGGAACAAGCATGGTTGGCTATAAAAAAGGTCTGGGCTTCAAAGTGGAATGAGAGAGCATACTTCAGCACAAGAAAAGTGAAATTAGACCATGATTATCTTTGCATGGCTGTCCTTGTTCAAGAAATAATAAATGCCGACTATGCATTCGTTATTCACACAACTAACCCATCTTCAGGAGACTCGTCGGAGATATATGCTGAGGTTGTCAAAGGTCTTGGAGAAACCTTAGTTGGAGCTTATCCTGGACGTGCCTTAAGTTTTATTAGCAAGAAAAATGCTCTGGACTCTCCTCAGGTGTTAGGTTACCCTAGCAAACCGATTGGCCTGTTTATCAGACAGTCCATAATCTTCCGATCCGATTCCAATGGTGAAGATCTTGAAGGTTATGCTGGTGCAGGCCTTTATGACAGTGTGCCTATGGATGCGGAAGAGGAAGTCGTGCTTGACTACTCATCTGACCCACTCATTACTGATGGGAACTTCCAAAAGACAATTCTGTCCAGCATTGCTCGCGCAGGAAGTGCAATCGAGGAGTTATATGATTCAGCACAAGACATCGAAGGTGTGATCAGAGATGGGAAACTCTATGTTGTCCAGACAAGACCCCAGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009628 GO:0009631 GO:0009941 GO:0009987 GO:0016052 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016781 GO:0031967 GO:0031975 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050521 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPDK_N

Protein Analysis

1406

Amino Acids

156.34

Weight (kDa)

6.06

Isoelectric Point (pI)

42.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ig_N_CWD1	PF23166	88 - 205	1.9e-40	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
DUF7067	PF23229	219 - 274	7.7e-28	Alpha-glucan water dikinase, DUF7067
Ig_N_CWD1	PF23166	364 - 482	5.6e-27	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
GWD1_pHisD	PF22973	949 - 1061	1.1e-53	Alpha-glucan water dikinase, phosphohistidine-like domain
PPDK_N	PF01326	1204 - 1280	8.9e-13	Pyruvate phosphate dikinase, AMP/ATP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10760 AT1G10760 AT1G10760
fragaria_vesca	FvH4_4g25800 FvH4_4g25800 FvH4_4g25800 FvH4_4g25800
malus_domestica	MD13G1096800.v1.1 MD16G1097800.v1.1
prunus_persica	Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1
pyrus_communis	pycom13g08450 pycom16g08330
rosa_chinensis	RchiOBHm_Chr4g0433251
rosa_laevigata	RLG00000006771
rosa_multiflora	Rmu_co8055492.1_g000001 Rmu_sc0010643.1_g000001
rosa_roxburghii	Rroxscaffold_5G00374810
rosa_rugosa	Rorug04G0267200 Rorug04G0267300 Rorug04G0267400 Rorug04G0267400
rosa_samantha	Rh4AG321900 Rh4BG329300 Rh4CG344600
rosa_wichuraiana	Rw4G027860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	4102
AatII	GACGTC	1 cut(s)	3024
Acc36I	ACCTGC	4 cut(s)	286, 1131, 1616, 2842
AccB7I	CCANNNNNTGG	1 cut(s)	854
AccI	GTMKAC	2 cut(s)	103, 1796
AccII	CGCG	2 cut(s)	127, 4119
AclI	AACGTT	1 cut(s)	1197
AcoI	YGGCCR	2 cut(s)	186, 2354
AcsI	RAATTY	6 cut(s)	152, 669, 1836, 2976, 3357, 3444
AcvI	CACGTG	1 cut(s)	1700
AcyI	GRCGYC	1 cut(s)	3021
AfaI	GTAC	9 cut(s)	231, 504, 713, 1131, 1257, 1498, 2226, 2489, 2705
AfiI	CCNNNNNNNGG	8 cut(s)	321, 854, 2243, 2323, 2442, 2607, 3581, 3779
AflII	CTTAAG	2 cut(s)	506, 3859
AflIII	ACRYGT	3 cut(s)	2290, 2583, 3074
AjiI	CACGTC	2 cut(s)	1808, 3854
AjnI	CCWGG	8 cut(s)	319, 776, 1263, 2263, 2514, 2646, 3580, 3847
AjuI	GAANNNNNNNTTGG	6 cut(s)	3459, 3491, 3819, 3851, 4083, 4115
AleI	CACNNNNGTG	1 cut(s)	2151
Alw21I	GWGCWC	3 cut(s)	466, 801, 2200
Alw26I	GTCTC	5 cut(s)	201, 355, 936, 3494, 3777
AlwNI	CAGNNNCTG	5 cut(s)	40, 326, 653, 2350, 2996
ApeKI	GCWGC	6 cut(s)	121, 746, 1577, 1663, 2272, 2780
ApoI	RAATTY	6 cut(s)	152, 669, 1836, 2976, 3357, 3444
ArsI	GACNNNNNNTTYG	6 cut(s)	1821, 1853, 2821, 2853, 3734, 3766
Asp700I	GAANNNNTTC	3 cut(s)	2037, 2976, 3399
AspLEI	GCGC	5 cut(s)	121, 129, 1546, 2653, 4121
AspS9I	GGNCC	2 cut(s)	2471, 2728
AsuC2I	CCSGG	1 cut(s)	262
AsuHPI	GGTGA	9 cut(s)	25, 374, 1124, 1496, 2216, 2978, 3596, 3605, 3986
AvaII	GGWCC	1 cut(s)	2728
AxyI	CCTNAGG	1 cut(s)	3897
BaeGI	GKGCMC	1 cut(s)	2614
BalI	TGGCCA	2 cut(s)	188, 2356
BamHI	GGATCC	1 cut(s)	241
BanII	GRGCYC	3 cut(s)	466, 801, 1210
BbrPI	CACGTG	1 cut(s)	1700
BbsI	GAAGAC	6 cut(s)	126, 2030, 2093, 2834, 3222, 3554
Bbv12I	GWGCWC	3 cut(s)	466, 801, 2200
BbvCI	CCTCAGC	1 cut(s)	3804
BbvI	GCAGC	6 cut(s)	133, 758, 1589, 1675, 2259, 2767
BciT130I	CCWGG	8 cut(s)	321, 778, 1265, 2265, 2516, 2648, 3582, 3849
BclI	TGATCA	1 cut(s)	4173
BcnI	CCSGG	1 cut(s)	262
BcoDI	GTCTC	5 cut(s)	201, 355, 936, 3494, 3777
BfaI	CTAG	9 cut(s)	831, 1311, 1926, 2367, 2507, 2633, 3095, 3185, 3915
BfmI	CTRYAG	3 cut(s)	141, 540, 1102
BfoI	RGCGCY	1 cut(s)	2654
BfrI	CTTAAG	2 cut(s)	506, 3859
BfuAI	ACCTGC	4 cut(s)	286, 1131, 1616, 2842
BglII	AGATCT	3 cut(s)	1528, 2158, 3979
BisI	GCNGC	9 cut(s)	122, 125, 595, 747, 882, 1578, 1664, 2273, 2781
BlsI	GCNGC	9 cut(s)	123, 126, 596, 748, 883, 1579, 1665, 2274, 2782
Bme1390I	CCNGG	9 cut(s)	262, 321, 778, 1265, 2265, 2516, 2648, 3582, 3849
Bme18I	GGWCC	1 cut(s)	2728
BmgBI	CACGTC	2 cut(s)	1808, 3854
BmgT120I	GGNCC	2 cut(s)	2471, 2728
BmiI	GGNNCC	5 cut(s)	243, 442, 493, 846, 1242
BmrFI	CCNGG	9 cut(s)	262, 321, 778, 1265, 2265, 2516, 2648, 3582, 3849
BmrI	ACTGGG	2 cut(s)	2617, 2858
BmsI	GCATC	8 cut(s)	298, 817, 1468, 1570, 1594, 1912, 3546, 4018
BmuI	ACTGGG	2 cut(s)	2617, 2858
BpiI	GAAGAC	6 cut(s)	126, 2030, 2093, 2834, 3222, 3554
BpmI	CTGGAG	5 cut(s)	14, 303, 1248, 1553, 1724
Bpu10I	CCTNAGC	2 cut(s)	2199, 3804
BpuEI	CTTGAG	2 cut(s)	2183, 2354
BpuMI	CCSGG	1 cut(s)	262
BsaAI	YACGTR	2 cut(s)	1700, 3314
BsaHI	GRCGYC	1 cut(s)	3021
BsaI	GGTCTC	2 cut(s)	201, 355
BsaWI	WCCGGW	1 cut(s)	3007
Bsc4I	CCNNNNNNNGG	8 cut(s)	321, 854, 2243, 2323, 2442, 2607, 3581, 3779
Bse1I	ACTGG	7 cut(s)	922, 980, 1415, 2003, 2612, 2816, 2864
Bse21I	CCTNAGG	1 cut(s)	3897
Bse3DI	GCAATG	4 cut(s)	1572, 2357, 2406, 4110
BseBI	CCWGG	8 cut(s)	321, 778, 1265, 2265, 2516, 2648, 3582, 3849
BseGI	GGATG	9 cut(s)	1083, 1483, 1647, 1759, 1791, 1828, 2570, 3219, 4033
BseLI	CCNNNNNNNGG	8 cut(s)	321, 854, 2243, 2323, 2442, 2607, 3581, 3779
BseMI	GCAATG	4 cut(s)	1572, 2357, 2406, 4110
BseMII	CTCAG	7 cut(s)	674, 747, 1443, 2163, 2213, 3795, 3911
BseNI	ACTGG	7 cut(s)	922, 980, 1415, 2003, 2612, 2816, 2864
BseRI	GAGGAG	3 cut(s)	1827, 3885, 4151
BseSI	GKGCMC	1 cut(s)	2614
BseXI	GCAGC	6 cut(s)	133, 758, 1589, 1675, 2259, 2767
BseYI	CCCAGC	2 cut(s)	2468, 3223
BsgI	GTGCAG	2 cut(s)	2175, 4020
Bsh1236I	CGCG	2 cut(s)	127, 4119
BsiHKAI	GWGCWC	3 cut(s)	466, 801, 2200
BsiSI	CCGG	3 cut(s)	261, 2474, 3008
BslFI	GGGAC	2 cut(s)	337, 1842
BslI	CCNNNNNNNGG	8 cut(s)	321, 854, 2243, 2323, 2442, 2607, 3581, 3779
BsmAI	GTCTC	5 cut(s)	201, 355, 936, 3494, 3777
BsmFI	GGGAC	2 cut(s)	337, 1842
BsmI	GAATGC	4 cut(s)	69, 247, 3576, 3749
Bso31I	GGTCTC	2 cut(s)	201, 355
Bsp1286I	GDGCHC	5 cut(s)	466, 801, 1210, 2200, 2614
Bsp1407I	TGTACA	1 cut(s)	711
Bsp19I	CCATGG	1 cut(s)	189
BspCNI	CTCAG	7 cut(s)	675, 748, 1442, 2162, 2212, 3796, 3910
BspFNI	CGCG	2 cut(s)	127, 4119
BspLI	GGNNCC	5 cut(s)	243, 442, 493, 846, 1242
BspMI	ACCTGC	4 cut(s)	286, 1131, 1616, 2842
BspTI	CTTAAG	2 cut(s)	506, 3859
BspTNI	GGTCTC	2 cut(s)	201, 355
BsrDI	GCAATG	4 cut(s)	1572, 2357, 2406, 4110
BsrGI	TGTACA	1 cut(s)	711
BsrI	ACTGG	7 cut(s)	922, 980, 1415, 2003, 2612, 2816, 2864
BssNAI	GTATAC	1 cut(s)	104
BssNI	GRCGYC	1 cut(s)	3021
BssT1I	CCWWGG	6 cut(s)	189, 876, 1053, 1423, 2707, 3575
Bst1107I	GTATAC	1 cut(s)	104
Bst2UI	CCWGG	8 cut(s)	321, 778, 1265, 2265, 2516, 2648, 3582, 3849
Bst6I	CTCTTC	2 cut(s)	452, 4029
BstACI	GRCGYC	1 cut(s)	3021
BstAFI	CTTAAG	2 cut(s)	506, 3859
BstAPI	GCANNNNNTGC	4 cut(s)	40, 1604, 2618, 4127
BstAUI	TGTACA	1 cut(s)	711
BstBAI	YACGTR	2 cut(s)	1700, 3314
BstC8I	GCNNGC	8 cut(s)	117, 186, 1382, 1609, 1736, 2655, 4003, 4117
BstDSI	CCRYGG	2 cut(s)	189, 237
BstEII	GGTNACC	1 cut(s)	3908
BstENI	CCTNNNNNAGG	1 cut(s)	2321
BstF5I	GGATG	9 cut(s)	1083, 1483, 1647, 1759, 1791, 1828, 2570, 3219, 4033
BstFNI	CGCG	2 cut(s)	127, 4119
BstH2I	RGCGCY	1 cut(s)	2654
BstHHI	GCGC	5 cut(s)	121, 129, 1546, 2653, 4121
BstMAI	GTCTC	5 cut(s)	201, 355, 936, 3494, 3777
BstNI	CCWGG	8 cut(s)	321, 778, 1265, 2265, 2516, 2648, 3582, 3849
BstNSI	RCATGY	5 cut(s)	971, 1384, 2294, 2587, 3078
BstPI	GGTNACC	1 cut(s)	3908
BstSCI	CCNGG	9 cut(s)	260, 319, 776, 1263, 2263, 2514, 2646, 3580, 3847
BstSFI	CTRYAG	3 cut(s)	141, 540, 1102
BstSLI	GKGCMC	1 cut(s)	2614
BstUI	CGCG	2 cut(s)	127, 4119
BstV1I	GCAGC	6 cut(s)	133, 758, 1589, 1675, 2259, 2767
BstV2I	GAAGAC	6 cut(s)	126, 2030, 2093, 2834, 3222, 3554
BstX2I	RGATCY	7 cut(s)	241, 1528, 1848, 2158, 2389, 2542, 3979
BstYI	RGATCY	7 cut(s)	241, 1528, 1848, 2158, 2389, 2542, 3979
BstZ17I	GTATAC	1 cut(s)	104
Bsu36I	CCTNAGG	1 cut(s)	3897
BtgI	CCRYGG	2 cut(s)	189, 237
BtrI	CACGTC	2 cut(s)	1808, 3854
BtsCI	GGATG	9 cut(s)	1083, 1483, 1647, 1759, 1791, 1828, 2570, 3219, 4033
BtsIMutI	CAGTG	3 cut(s)	2200, 2907, 4021
BveI	ACCTGC	4 cut(s)	286, 1131, 1616, 2842
Cac8I	GCNNGC	8 cut(s)	117, 186, 1382, 1609, 1736, 2655, 4003, 4117
CaiI	CAGNNNCTG	5 cut(s)	40, 326, 653, 2350, 2996
CfoI	GCGC	5 cut(s)	121, 129, 1546, 2653, 4121
Cfr13I	GGNCC	2 cut(s)	2471, 2728
CseI	GACGC	1 cut(s)	2087
Csp6I	GTAC	9 cut(s)	230, 503, 712, 1130, 1256, 1497, 2225, 2488, 2704
CspCI	CAANNNNNGTGG	2 cut(s)	3513, 3548
CviQI	GTAC	9 cut(s)	230, 503, 712, 1130, 1256, 1497, 2225, 2488, 2704
DraI	TTTAAA	1 cut(s)	2136
DrdI	GACNNNNNNGTC	1 cut(s)	4102
DseDI	GACNNNNNNGTC	1 cut(s)	4102
EaeI	YGGCCR	2 cut(s)	186, 2354
Eam1104I	CTCTTC	2 cut(s)	452, 4029
EarI	CTCTTC	2 cut(s)	452, 4029
EciI	GGCGGA	1 cut(s)	1899
Ecl136II	GAGCTC	2 cut(s)	464, 799
Eco130I	CCWWGG	6 cut(s)	189, 876, 1053, 1423, 2707, 3575
Eco147I	AGGCCT	2 cut(s)	2371, 4005
Eco24I	GRGCYC	3 cut(s)	466, 801, 1210
Eco31I	GGTCTC	2 cut(s)	201, 355
Eco32I	GATATC	2 cut(s)	636, 1180
Eco47I	GGWCC	1 cut(s)	2728
Eco53kI	GAGCTC	2 cut(s)	464, 799
Eco72I	CACGTG	1 cut(s)	1700
Eco81I	CCTNAGG	1 cut(s)	3897
Eco91I	GGTNACC	1 cut(s)	3908
EcoICRI	GAGCTC	2 cut(s)	464, 799
EcoNI	CCTNNNNNAGG	1 cut(s)	2321
EcoO65I	GGTNACC	1 cut(s)	3908
EcoRI	GAATTC	2 cut(s)	1836, 3357
EcoRII	CCWGG	8 cut(s)	319, 776, 1263, 2263, 2514, 2646, 3580, 3847
EcoRV	GATATC	2 cut(s)	636, 1180
EcoT14I	CCWWGG	6 cut(s)	189, 876, 1053, 1423, 2707, 3575
EcoT22I	ATGCAT	2 cut(s)	1563, 3751
EcoT38I	GRGCYC	3 cut(s)	466, 801, 1210
ErhI	CCWWGG	6 cut(s)	189, 876, 1053, 1423, 2707, 3575
FaqI	GGGAC	2 cut(s)	337, 1842
FauI	CCCGC	1 cut(s)	2651
FauNDI	CATATG	1 cut(s)	355
FbaI	TGATCA	1 cut(s)	4173
FblI	GTMKAC	2 cut(s)	103, 1796
Fnu4HI	GCNGC	9 cut(s)	122, 125, 595, 747, 882, 1578, 1664, 2273, 2781
FokI	GGATG	9 cut(s)	1070, 1490, 1654, 1746, 1798, 1835, 2577, 3206, 4040
FriOI	GRGCYC	3 cut(s)	466, 801, 1210
Fsp4HI	GCNGC	9 cut(s)	122, 125, 595, 747, 882, 1578, 1664, 2273, 2781
FspBI	CTAG	9 cut(s)	831, 1311, 1926, 2367, 2507, 2633, 3095, 3185, 3915
GlaI	GCGC	5 cut(s)	120, 128, 1545, 2652, 4120
GluI	GCNGC	9 cut(s)	122, 125, 595, 747, 882, 1578, 1664, 2273, 2781
GsaI	CCCAGC	2 cut(s)	2472, 3227
GsuI	CTGGAG	5 cut(s)	14, 303, 1248, 1553, 1724
HaeII	RGCGCY	1 cut(s)	2654
HapII	CCGG	3 cut(s)	261, 2474, 3008
HgaI	GACGC	1 cut(s)	2087
HhaI	GCGC	5 cut(s)	121, 129, 1546, 2653, 4121
Hin1I	GRCGYC	1 cut(s)	3021
Hin6I	GCGC	5 cut(s)	119, 127, 1544, 2651, 4119
HinP1I	GCGC	5 cut(s)	119, 127, 1544, 2651, 4119
HincII	GTYRAC	1 cut(s)	3516
HindII	GTYRAC	1 cut(s)	3516
HindIII	AAGCTT	2 cut(s)	2717, 3108
HpaII	CCGG	3 cut(s)	261, 2474, 3008
HphI	GGTGA	9 cut(s)	25, 374, 1124, 1496, 2216, 2978, 3596, 3605, 3986
Hpy99I	CGWCG	3 cut(s)	204, 432, 3793
HpyCH4IV	ACGT	7 cut(s)	1197, 1699, 1807, 2035, 3021, 3313, 3853
HpySE526I	ACGT	7 cut(s)	1197, 1699, 1807, 2035, 3021, 3313, 3853
Hsp92I	GRCGYC	1 cut(s)	3021
HspAI	GCGC	5 cut(s)	119, 127, 1544, 2651, 4119
Ksp22I	TGATCA	1 cut(s)	4173
LmnI	GCTCC	7 cut(s)	497, 691, 804, 1743, 2347, 3302, 3839
Lsp1109I	GCAGC	6 cut(s)	133, 758, 1589, 1675, 2259, 2767
LweI	GCATC	8 cut(s)	298, 817, 1468, 1570, 1594, 1912, 3546, 4018
MaeI	CTAG	9 cut(s)	831, 1311, 1926, 2367, 2507, 2633, 3095, 3185, 3915
MaeII	ACGT	7 cut(s)	1197, 1699, 1807, 2035, 3021, 3313, 3853
MaeIII	GTNAC	9 cut(s)	87, 208, 281, 833, 860, 1135, 1976, 2189, 3908
MflI	RGATCY	7 cut(s)	241, 1528, 1848, 2158, 2389, 2542, 3979
MhlI	GDGCHC	5 cut(s)	466, 801, 1210, 2200, 2614
MlsI	TGGCCA	2 cut(s)	188, 2356
MluNI	TGGCCA	2 cut(s)	188, 2356
MlyI	GAGTC	5 cut(s)	2174, 2678, 3077, 3779, 3884
MmeI	TCCRAC	8 cut(s)	1218, 1783, 2200, 2857, 3280, 3334, 3771, 3817
Mox20I	TGGCCA	2 cut(s)	188, 2356
Mph1103I	ATGCAT	2 cut(s)	1563, 3751
MroXI	GAANNNNTTC	3 cut(s)	2037, 2976, 3399
MscI	TGGCCA	2 cut(s)	188, 2356
MslI	CAYNNNNRTG	3 cut(s)	384, 2151, 2498
Msp20I	TGGCCA	2 cut(s)	188, 2356
MspA1I	CMGCKG	2 cut(s)	1666, 2780
MspCI	CTTAAG	2 cut(s)	506, 3859
MspI	CCGG	3 cut(s)	261, 2474, 3008
MspR9I	CCNGG	9 cut(s)	262, 321, 778, 1265, 2265, 2516, 2648, 3582, 3849
Mva1269I	GAATGC	4 cut(s)	69, 247, 3576, 3749
MvaI	CCWGG	8 cut(s)	321, 778, 1265, 2265, 2516, 2648, 3582, 3849
MvnI	CGCG	2 cut(s)	127, 4119
NciI	CCSGG	1 cut(s)	262
NcoI	CCATGG	1 cut(s)	189
NdeI	CATATG	1 cut(s)	355
NlaIV	GGNNCC	5 cut(s)	243, 442, 493, 846, 1242
NmuCI	GTSAC	2 cut(s)	1135, 1976
NsiI	ATGCAT	2 cut(s)	1563, 3751
NspI	RCATGY	5 cut(s)	971, 1384, 2294, 2587, 3078
OliI	CACNNNNGTG	1 cut(s)	2151
PaeI	GCATGC	1 cut(s)	1384
PasI	CCCWGGG	1 cut(s)	1264
PceI	AGGCCT	2 cut(s)	2371, 4005
PciI	ACATGT	3 cut(s)	2290, 2583, 3074
PcsI	WCGNNNNNNNCGW	1 cut(s)	1116
PctI	GAATGC	4 cut(s)	69, 247, 3576, 3749
PdmI	GAANNNNTTC	3 cut(s)	2037, 2976, 3399
PflMI	CCANNNNNTGG	1 cut(s)	854
PfoI	TCCNGGA	2 cut(s)	2263, 3847
PkrI	GCNGC	9 cut(s)	123, 126, 596, 748, 883, 1579, 1665, 2274, 2782
PleI	GAGTC	5 cut(s)	2173, 2677, 3077, 3779, 3884
PmaCI	CACGTG	1 cut(s)	1700
PmlI	CACGTG	1 cut(s)	1700
PpsI	GAGTC	5 cut(s)	2173, 2677, 3077, 3779, 3884
Ppu21I	YACGTR	2 cut(s)	1700, 3314
PscI	ACATGT	3 cut(s)	2290, 2583, 3074
Psp124BI	GAGCTC	2 cut(s)	466, 801
Psp1406I	AACGTT	1 cut(s)	1197
Psp6I	CCWGG	8 cut(s)	319, 776, 1263, 2263, 2514, 2646, 3580, 3847
PspCI	CACGTG	1 cut(s)	1700
PspEI	GGTNACC	1 cut(s)	3908
PspFI	CCCAGC	2 cut(s)	2468, 3223
PspGI	CCWGG	8 cut(s)	319, 776, 1263, 2263, 2514, 2646, 3580, 3847
PspN4I	GGNNCC	5 cut(s)	243, 442, 493, 846, 1242
PspPI	GGNCC	2 cut(s)	2471, 2728
PstNI	CAGNNNCTG	5 cut(s)	40, 326, 653, 2350, 2996
PsuI	RGATCY	7 cut(s)	241, 1528, 1848, 2158, 2389, 2542, 3979
PvuII	CAGCTG	2 cut(s)	1666, 2780
RsaI	GTAC	9 cut(s)	231, 504, 713, 1131, 1257, 1498, 2226, 2489, 2705
RsaNI	GTAC	9 cut(s)	230, 503, 712, 1130, 1256, 1497, 2225, 2488, 2704
RseI	CAYNNNNRTG	3 cut(s)	384, 2151, 2498
SacI	GAGCTC	2 cut(s)	466, 801
SatI	GCNGC	9 cut(s)	122, 125, 595, 747, 882, 1578, 1664, 2273, 2781
Sau96I	GGNCC	2 cut(s)	2471, 2728
SchI	GAGTC	5 cut(s)	2174, 2678, 3077, 3779, 3884
ScrFI	CCNGG	9 cut(s)	262, 321, 778, 1265, 2265, 2516, 2648, 3582, 3849
SduI	GDGCHC	5 cut(s)	466, 801, 1210, 2200, 2614
SfaNI	GCATC	8 cut(s)	298, 817, 1468, 1570, 1594, 1912, 3546, 4018
SfcI	CTRYAG	3 cut(s)	141, 540, 1102
SinI	GGWCC	1 cut(s)	2728
SmiMI	CAYNNNNRTG	3 cut(s)	384, 2151, 2498
SmlI	CTYRAG	4 cut(s)	506, 2162, 2333, 3859
SmoI	CTYRAG	4 cut(s)	506, 2162, 2333, 3859
SphI	GCATGC	1 cut(s)	1384
SseBI	AGGCCT	2 cut(s)	2371, 4005
SspI	AATATT	3 cut(s)	1747, 3091, 3319
SspMI	CTAG	9 cut(s)	831, 1311, 1926, 2367, 2507, 2633, 3095, 3185, 3915
SstI	GAGCTC	2 cut(s)	466, 801
StuI	AGGCCT	2 cut(s)	2371, 4005
StyD4I	CCNGG	9 cut(s)	260, 319, 776, 1263, 2263, 2514, 2646, 3580, 3847
StyI	CCWWGG	6 cut(s)	189, 876, 1053, 1423, 2707, 3575
TaiI	ACGT	7 cut(s)	1200, 1702, 1810, 2038, 3024, 3316, 3856
TaqI	TCGA	6 cut(s)	1771, 1840, 2806, 3496, 4134, 4164
TatI	WGTACW	5 cut(s)	229, 711, 1255, 2224, 2487
TauI	GCSGC	3 cut(s)	127, 597, 884
TscAI	CASTG	3 cut(s)	2200, 2914, 4021
TseFI	GTSAC	2 cut(s)	1135, 1976
TseI	GCWGC	6 cut(s)	121, 746, 1577, 1663, 2272, 2780
Tsp45I	GTSAC	2 cut(s)	1135, 1976
TspGWI	ACGGA	4 cut(s)	254, 490, 1798, 2756
TspRI	CASTG	3 cut(s)	2200, 2914, 4021
Van91I	CCANNNNNTGG	1 cut(s)	854
Vha464I	CTTAAG	2 cut(s)	506, 3859
VpaK11BI	GGWCC	1 cut(s)	2728
XagI	CCTNNNNNAGG	1 cut(s)	2321
XapI	RAATTY	6 cut(s)	152, 669, 1836, 2976, 3357, 3444
XbaI	TCTAGA	2 cut(s)	2506, 2632
XceI	RCATGY	5 cut(s)	971, 1384, 2294, 2587, 3078
XmiI	GTMKAC	2 cut(s)	103, 1796
XmnI	GAANNNNTTC	3 cut(s)	2037, 2976, 3399
XspI	CTAG	9 cut(s)	831, 1311, 1926, 2367, 2507, 2633, 3095, 3185, 3915
ZraI	GACGTC	1 cut(s)	3022
Zsp2I	ATGCAT	2 cut(s)	1563, 3751

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.