Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0005249

Physical Location & Seq

Genomic Coordinates Reverse (-)

10315838 .. 10328104

Gene Structure 12267 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000006771

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4203 bp

ATGCGTGCAGGGACTGAATCTAAGAAATCCACGGCTCGTATCACAGTATTTCCCAGAACTGTAAAGTGTGTCGAGGAATATGAGCAACTCGTGCCAGCGCAGCTGCGCAAGTCTTCTTCTATATCCAAGAAATTTTGTGGCAACGGTTTGCAGAAGCCGAAGCTGGCCATGGCGTCTCGACGACCTGTTACATTTGTTCCTTGCGCTGTACTGACCACGGATCCGCATTCTGATCAGCTTGCCGGGAAATACAACCTTGATGGTAACATTGAAATGCAGGTTTATGTTGATGCTTCCTCTCCAGGGTCTGCCACTAAAGTAGATATTCAGGTCTCATATGGCGGTGATTCTTTGGTTCTTCATTGGGGTGGGATAAAAGATAGAAAAGAAAAATGGGTGCTTCCTTCTCGTCGCCCAGATGGAACCAAAGAATATAAGAAGAGAGCTCTTAGAACTCCTTTTGCGAAATCTGGCTCCAATTCCGTACTTAAAATTGAGATTGATGATCCTGCAATACAAGCTATAGAGTTTCTCATAGTTGATGAAAGTCAGAACAGATGGTTTAAGAACAATGGTGGCAACTTTCATGTTAAGTTACCTTTGAAAGAGAAGTTGATATCAAATGTTTCAGTTCCTGAAGATTTAGTGCAAATTCAAGCATATCTGAGGTGGGAGCGAAAGGGTAAACAGATGTATACACCGGAGCAAGAGAAGGTGGAGTATGAAGCAGCCCGAACTGAGCTATTGGAGGAAGTAGCCAGGGGTGCTTCCATACAAGAGCTCCAAGCAAGGCTGACTAAGAAAACTGATGCTAGTAACAGTCAGGAACCATCTCATGCTGGTAACAAAGAAGTTTCAAAGGCCGCAAAGAAACATGAGAGTAAAAAGGTTTTCAGAACTGAAAGAATTAAGCGGAAGCAACGAGATTTAATGAAGATTCTCAACAAACATGCTACTAAACCTGTAGATGAAGCTAAACTTGCACATGAAGAATCAAGGAAACCAAAAGCCTTGAAAGCAGTTGAGCTATTTGCCAAAGCAAAAGAAGAACAAGATGGTGCTTCCGTCCTGAACAAAAAAATCTACAGGCTCGGTGATAAGGACCTGCTGGTACTTGTGACAAAGGTTGCTGCTAAGACAAAGGTTCATCTGGCAACAGATATCAAAGAGCCAATTAACCTTCATTGGGCTCTATCTGTAAACAAAGCTGGGGAGTGGTTGGAACCACCTCCAAATGTACTGCCCCAGGGTTCAGTTTCTCTAAATGGGTCTGTTGAAACACAATTTGTATCTAGTTCTGTTGATTCTACTTATGAGGTTCAATCTTTAGAAATAGAAATGCAAGAGGAAAGTTTCAAAGGAATGCCATTTGTCCTTTGTTCTTCTGGAAACTGGATTAAGAACCAAGGATCAGATTTCTACGCTGACTTTGGTGTTGCGGTCAAGAAAGTTCAGAAGGATGCTGGTGATGGAAAAGGTGCTGCAAAGGATTTATTAGATAAAATAGCAGGTCTGGAGAGTGAGGCCCAAAAATCCTTTATGCATCGGTTTAACATTGCAGCAGACTTGATAAATCAAGCAAAAGATGCTGGCAAATTAGGTCTTGCAGGTATTCTGGTGTGGATGAGGTTTATGGCTATGAGGCAGCTGATTTGGAATAAAAATTACAACGTCAAACCACGTGAGATTAGTAAAGCACAGGACAGGCTCACAGGCTTGCTCCAGAATATTTACACAAGTTATCCACACTATCGAGAGCTTCTACGGTTGATATTGTCTACTGTTGGACGTGGAGGAGAAGGGGATGTGGGGCAGCGAATTCGAGATGAGATTCTTGTTATCCAGAGAAACTGTGATTGTAAGGGCGGAATGATGGAGGAATGGCATCAAAAGTTGCATAATAACACTAGCCCAGATGATGTTGTTATATGTCAGGCACTAATTGATTACATCAAAAGTGACTTTGATATTGGTGTTTACTGGAAAACTTTGAACGAGAATGGAATTACAAAAGAACGTCTACTAAGCTATGACCGTGCAATCCACAATGAACCAAGTTTCAGGGCAGATCAGAAAGAGCGTCTTCTGCATGATCTGGGGAACTACATGAGAACTTTAAAGGCAGTTCACTCAGGTGCAGATCTTGAGTCTGCCATCACAAATTGTTTGGGTTACAGTGCTCAGGGTCAAGGCTTCATGGTTGGAGTACAAATAAATCCTGTATCTGGCCTGCCCCCTGAAGTTCCAGGACTGCTGCAGTTTGTAATGGAACATGTTGAGGACAAGAATGTGGAAGTCCTTATTGAGGGTTTGCTTGAGGCTCGTCAGGAGCTGTGGCCATTGCTCTCCAAGCCTAATGATCGCCTGAAGGATCTCTTATTTTTGGACATTGCCCTTGATTCTACTGTGAGAACCGCCATTGAGAGGGGATATGAAGAACTGAATAATGCTGGGCCGGAGAAAATTATGTACTTCATCTCAATGGTTCTAGAAAACCTGGCATTATCATCAGATGATAATGAGGATCTCCTCTACTGTTTGAAGGGTTGGAATCATGCGTTAAACATGTTAAAGAGTAATACCGATAACTGGGCATTATATGCAAAATCAATCCTAGACAGAACCCGCCTGGCGCTTGCAAACAAGGCAGAGTCGTATCACGGTATTTTACAGCCATCAGCAGAGTACCTTGGATCAAAGCTTGGAGTGGACCAACGGGCTTTGAGCATATTTACTGAAGAAGTTATCCGAGCTGGATCAGCTGCCTCTTTGTCTGCACTCGTTAATCGACTTGATCCAGTTCTTCGGAAGACTGCTAATTTAGGAAGTTGGCAGGTTATCAGCCCAGTTGAAGTGGTTGGATATGTTGTAGTTGTGGACGAGTTGCTCACTGTCCAGAATAAAGTATATGAGAAGCCGACTATTTTGGTGGCAAGAAGTGTGAGAGGTGAGGAAGAAATTCCAGATGGCACAGTTGCTGTGCTAACACCTGATATGCCAGACGTCCTATCTCATGTTTCTGTCCGAGCAAGAAATAGCAAGGTTTGTTTTGCGACATGTTTTGACTCCAATATTCTAGCTGACCTCCAAGCTTGTAAAGGGAAGTTATTGCGTGTAAAGCCAACTCCTGCTGATGTAGTTTATAGCGAGGTTAATGAGAGTGAGCTAGGAGATGCAAGTTCAACTAATTCAAATGAAGACATCCCAGCTCTGACATTAGTGAAGAAACAGTTTACGGGTAGATATGCCATATCATCTGATGAGTTCACAAGTGAAATGGTCGGAGCTAAATCACGTAATATTTCTTATATAAAAGGAAAACTACCCTCTTGGGTTGGAATTCCTACATCAGTTGCCCTACCATTTGGAGTATTTGAAAAAGTTCTTTCTGAAGATTCAAATAAGGCTGTTGCTGACAAGTTAGAAATTCTAAATAAAAAGTTAAAAGAAGGAGATTTTGGTTCACTTGGGGAGATTCGAGAGACAGTTTTACAGTTGACAGCACCACCCCCGTTGGTGCAAGAGCTGAAGACTAGGATGCAAAGTTCAGGAATGCCTTGGCCTGGTGATGAGGGTGAACAACGATGGGAACAAGCATGGTTGGCTATAAAAAAGGTCTGGGCTTCAAAGTGGAATGAGAGAGCATACTTCAGCACAAGAAAAGTGAAATTAGACCATGATTATCTTTGCATGGCTGTCCTTGTTCAAGAAATAATAAATGCCGACTATGCATTCGTTATTCACACAACTAACCCATCTTCAGGAGACTCGTTGGAGATATATGCTGAGGTTGTCAAAGGTCTTGGAGAAACCTTAGTTGGAGCTTATCCTGGACGTGCTTTAAGTTTTATTAGCAAGAAAAATGCTCTGGACTCTCCTCAGGTGTTAGGTTACCCTAGCAAACCGATTGGCCTCTTTATCAGACGGTCCATAATCTTCCGATCCGATTCCAATGGTGAAGATCTTGAAGGTTATGCTGGTGCAGGCCTTTATGACAGTGTGCCTATGGATGCGGAAGAGGAAGTCGTGCTTGACTACTCATCTGACCCACTCGTTACTGATGGGAACTTCCAAAAGACAATTCTGTCCAGCATTGCTCGCGCAGGAAGTGCAATTGAGGAGTTATATGGTTCAGCACAAGACATCGAAGGTGTGATCAGAGATGGGAAACTCTATGTTGTCCAGACAAGACCGCAGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009628 GO:0009631 GO:0009941 GO:0009987 GO:0016052 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016781 GO:0031967 GO:0031975 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050521 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPDK_N

Protein Analysis

1401

Amino Acids

155.72

Weight (kDa)

6.5

Isoelectric Point (pI)

39.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ig_N_CWD1	PF23166	82 - 199	9e-41	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
DUF7067	PF23229	213 - 268	4.1e-28	Alpha-glucan water dikinase, DUF7067
Ig_N_CWD1	PF23166	358 - 476	1.7e-26	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
GWD1_pHisD	PF22973	943 - 1055	3.6e-53	Alpha-glucan water dikinase, phosphohistidine-like domain
PPDK_N	PF01326	1198 - 1274	3.9e-12	Pyruvate phosphate dikinase, AMP/ATP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10760 AT1G10760 AT1G10760
fragaria_vesca	FvH4_4g25800 FvH4_4g25800 FvH4_4g25800 FvH4_4g25800
malus_domestica	MD13G1096800.v1.1 MD16G1097800.v1.1
prunus_persica	Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1
pyrus_communis	pycom13g08450 pycom16g08330
rosa_chinensis	RchiOBHm_Chr4g0433251
rosa_laevigata	RLG00000006771
rosa_multiflora	Rmu_co8055492.1_g000001 Rmu_sc0010643.1_g000001
rosa_roxburghii	Rroxscaffold_5G00374810
rosa_rugosa	Rorug04G0267200 Rorug04G0267300 Rorug04G0267400 Rorug04G0267400
rosa_samantha	Rh4AG321900 Rh4BG329300 Rh4CG344600
rosa_wichuraiana	Rw4G027860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	4084
AatII	GACGTC	1 cut(s)	3006
Acc16I	TGCGCA	1 cut(s)	107
Acc36I	ACCTGC	5 cut(s)	268, 1113, 1499, 1598, 2824
AccI	GTMKAC	3 cut(s)	695, 1778, 2020
AccII	CGCG	1 cut(s)	4101
AcoI	YGGCCR	2 cut(s)	165, 2336
AcsI	RAATTY	6 cut(s)	131, 651, 1818, 2958, 3339, 3426
AcuI	CTGAAG	8 cut(s)	657, 2259, 2387, 2757, 3411, 3548, 3634, 3744
AcvI	CACGTG	1 cut(s)	1682
AcyI	GRCGYC	2 cut(s)	173, 3003
AfaI	GTAC	7 cut(s)	210, 486, 1113, 1239, 2208, 2471, 2687
AfiI	CCNNNNNNNGG	8 cut(s)	303, 1186, 2225, 2305, 2424, 2589, 3563, 3761
AflIII	ACRYGT	3 cut(s)	2272, 2565, 3056
AjiI	CACGTC	2 cut(s)	1790, 3836
AjnI	CCWGG	8 cut(s)	301, 758, 1245, 2245, 2496, 2628, 3562, 3829
AjuI	GAANNNNNNNTTGG	6 cut(s)	3441, 3473, 3801, 3833, 4065, 4097
AleI	CACNNNNGTG	1 cut(s)	2133
AloI	GAACNNNNNNTCC	2 cut(s)	3529, 3561
Alw21I	GWGCWC	3 cut(s)	448, 783, 2182
Alw26I	GTCTC	4 cut(s)	180, 337, 3476, 3759
AlwNI	CAGNNNCTG	5 cut(s)	14, 308, 635, 2332, 2978
AoxI	GGCC	9 cut(s)	165, 861, 1524, 2227, 2336, 2453, 3560, 3910, 3985
ApoI	RAATTY	6 cut(s)	131, 651, 1818, 2958, 3339, 3426
ArsI	GACNNNNNNTTYG	2 cut(s)	3716, 3748
Asp700I	GAANNNNTTC	2 cut(s)	2958, 3381
AspLEI	GCGC	5 cut(s)	100, 108, 206, 2635, 4103
AspS9I	GGNCC	5 cut(s)	1102, 1525, 2453, 2710, 3927
AsuC2I	CCSGG	1 cut(s)	244
AsuHPI	GGTGA	7 cut(s)	356, 1106, 1478, 2960, 3578, 3587, 3968
AvaII	GGWCC	3 cut(s)	1102, 2710, 3927
AxyI	CCTNAGG	1 cut(s)	3879
BalI	TGGCCA	2 cut(s)	167, 2338
BamHI	GGATCC	1 cut(s)	220
BanII	GRGCYC	3 cut(s)	448, 783, 1192
BauI	CACGAG	1 cut(s)	89
BbrPI	CACGTG	1 cut(s)	1682
BbsI	GAAGAC	5 cut(s)	105, 2075, 2816, 3204, 3536
Bbv12I	GWGCWC	3 cut(s)	448, 783, 2182
BbvCI	CCTCAGC	1 cut(s)	3786
BceAI	ACGGC	1 cut(s)	48
BcgI	CGANNNNNNTGC	2 cut(s)	1802, 1836
BciT130I	CCWGG	8 cut(s)	303, 760, 1247, 2247, 2498, 2630, 3564, 3831
BclI	TGATCA	2 cut(s)	232, 4155
BcnI	CCSGG	1 cut(s)	244
BcoDI	GTCTC	4 cut(s)	180, 337, 3476, 3759
BfaI	CTAG	9 cut(s)	813, 1293, 1908, 2489, 2615, 3077, 3167, 3534, 3897
BfmI	CTRYAG	4 cut(s)	522, 963, 1084, 2255
BfoI	RGCGCY	1 cut(s)	2636
BfuAI	ACCTGC	5 cut(s)	268, 1113, 1499, 1598, 2824
BglII	AGATCT	2 cut(s)	2140, 3961
Bme1390I	CCNGG	9 cut(s)	244, 303, 760, 1247, 2247, 2498, 2630, 3564, 3831
Bme18I	GGWCC	3 cut(s)	1102, 2710, 3927
BmgBI	CACGTC	2 cut(s)	1790, 3836
BmgT120I	GGNCC	5 cut(s)	1102, 1525, 2453, 2710, 3927
BmiI	GGNNCC	5 cut(s)	222, 424, 475, 828, 1224
BmrFI	CCNGG	9 cut(s)	244, 303, 760, 1247, 2247, 2498, 2630, 3564, 3831
BmrI	ACTGGG	2 cut(s)	2599, 2840
BmsI	GCATC	9 cut(s)	280, 799, 1450, 1552, 1576, 1894, 3163, 3528, 4000
BmuI	ACTGGG	2 cut(s)	2599, 2840
BpiI	GAAGAC	5 cut(s)	105, 2075, 2816, 3204, 3536
BpmI	CTGGAG	3 cut(s)	285, 1535, 1706
Bpu10I	CCTNAGC	2 cut(s)	2181, 3786
BpuEI	CTTGAG	2 cut(s)	2165, 2336
BpuMI	CCSGG	1 cut(s)	244
BsaAI	YACGTR	2 cut(s)	1682, 3296
BsaHI	GRCGYC	2 cut(s)	173, 3003
BsaI	GGTCTC	1 cut(s)	337
BsaWI	WCCGGW	1 cut(s)	700
BsaXI	ACNNNNNCTCC	2 cut(s)	765, 795
Bsc4I	CCNNNNNNNGG	8 cut(s)	303, 1186, 2225, 2305, 2424, 2589, 3563, 3761
Bse1I	ACTGG	5 cut(s)	1397, 1985, 2594, 2798, 2846
Bse21I	CCTNAGG	1 cut(s)	3879
Bse3DI	GCAATG	4 cut(s)	1554, 2339, 2388, 4092
BseBI	CCWGG	8 cut(s)	303, 760, 1247, 2247, 2498, 2630, 3564, 3831
BseGI	GGATG	6 cut(s)	1465, 1629, 1810, 3201, 3543, 4015
BseLI	CCNNNNNNNGG	8 cut(s)	303, 1186, 2225, 2305, 2424, 2589, 3563, 3761
BseMI	GCAATG	4 cut(s)	1554, 2339, 2388, 4092
BseMII	CTCAG	6 cut(s)	656, 729, 2145, 2195, 3777, 3893
BseNI	ACTGG	5 cut(s)	1397, 1985, 2594, 2798, 2846
BseRI	GAGGAG	4 cut(s)	1809, 2519, 3867, 4133
BseYI	CCCAGC	3 cut(s)	1208, 2450, 3205
BsgI	GTGCAG	4 cut(s)	27, 2157, 2760, 4002
Bsh1236I	CGCG	1 cut(s)	4101
BshFI	GGCC	9 cut(s)	167, 863, 1526, 2229, 2338, 2455, 3562, 3912, 3987
BsiHKAI	GWGCWC	3 cut(s)	448, 783, 2182
BsiSI	CCGG	3 cut(s)	243, 701, 2456
BslFI	GGGAC	1 cut(s)	25
BslI	CCNNNNNNNGG	8 cut(s)	303, 1186, 2225, 2305, 2424, 2589, 3563, 3761
BsmAI	GTCTC	4 cut(s)	180, 337, 3476, 3759
BsmBI	CGTCTC	1 cut(s)	180
BsmFI	GGGAC	1 cut(s)	25
BsmI	GAATGC	4 cut(s)	226, 1368, 3558, 3731
BsnI	GGCC	9 cut(s)	167, 863, 1526, 2229, 2338, 2455, 3562, 3912, 3987
Bso31I	GGTCTC	1 cut(s)	337
Bsp1286I	GDGCHC	4 cut(s)	448, 783, 1192, 2182
Bsp19I	CCATGG	1 cut(s)	168
BspANI	GGCC	9 cut(s)	167, 863, 1526, 2229, 2338, 2455, 3562, 3912, 3987
BspCNI	CTCAG	6 cut(s)	657, 730, 2144, 2194, 3778, 3892
BspFNI	CGCG	1 cut(s)	4101
BspLI	GGNNCC	5 cut(s)	222, 424, 475, 828, 1224
BspMAI	CTGCAG	1 cut(s)	2259
BspMI	ACCTGC	5 cut(s)	268, 1113, 1499, 1598, 2824
BspTNI	GGTCTC	1 cut(s)	337
BsrDI	GCAATG	4 cut(s)	1554, 2339, 2388, 4092
BsrI	ACTGG	5 cut(s)	1397, 1985, 2594, 2798, 2846
BssNAI	GTATAC	1 cut(s)	696
BssNI	GRCGYC	2 cut(s)	173, 3003
BssSI	CACGAG	1 cut(s)	89
BssT1I	CCWWGG	4 cut(s)	168, 1405, 2689, 3557
Bst1107I	GTATAC	1 cut(s)	696
Bst2BI	CACGAG	1 cut(s)	89
Bst2UI	CCWGG	8 cut(s)	303, 760, 1247, 2247, 2498, 2630, 3564, 3831
Bst6I	CTCTTC	2 cut(s)	434, 4011
BstACI	GRCGYC	2 cut(s)	173, 3003
BstAPI	GCANNNNNTGC	4 cut(s)	91, 1586, 2600, 4109
BstBAI	YACGTR	2 cut(s)	1682, 3296
BstDSI	CCRYGG	3 cut(s)	30, 168, 216
BstEII	GGTNACC	1 cut(s)	3890
BstENI	CCTNNNNNAGG	1 cut(s)	2303
BstF5I	GGATG	6 cut(s)	1465, 1629, 1810, 3201, 3543, 4015
BstFNI	CGCG	1 cut(s)	4101
BstH2I	RGCGCY	1 cut(s)	2636
BstHHI	GCGC	5 cut(s)	100, 108, 206, 2635, 4103
BstMAI	GTCTC	4 cut(s)	180, 337, 3476, 3759
BstNI	CCWGG	8 cut(s)	303, 760, 1247, 2247, 2498, 2630, 3564, 3831
BstNSI	RCATGY	4 cut(s)	953, 2276, 2569, 3060
BstPI	GGTNACC	1 cut(s)	3890
BstSCI	CCNGG	9 cut(s)	242, 301, 758, 1245, 2245, 2496, 2628, 3562, 3829
BstSFI	CTRYAG	4 cut(s)	522, 963, 1084, 2255
BstUI	CGCG	1 cut(s)	4101
BstV2I	GAAGAC	5 cut(s)	105, 2075, 2816, 3204, 3536
BstX2I	RGATCY	5 cut(s)	220, 2140, 2371, 2524, 3961
BstYI	RGATCY	5 cut(s)	220, 2140, 2371, 2524, 3961
BstZ17I	GTATAC	1 cut(s)	696
Bsu36I	CCTNAGG	1 cut(s)	3879
BsuRI	GGCC	9 cut(s)	167, 863, 1526, 2229, 2338, 2455, 3562, 3912, 3987
BtgI	CCRYGG	3 cut(s)	30, 168, 216
BtrI	CACGTC	2 cut(s)	1790, 3836
BtsCI	GGATG	6 cut(s)	1465, 1629, 1810, 3201, 3543, 4015
BtsIMutI	CAGTG	3 cut(s)	2182, 2889, 4003
BveI	ACCTGC	5 cut(s)	268, 1113, 1499, 1598, 2824
CaiI	CAGNNNCTG	5 cut(s)	14, 308, 635, 2332, 2978
CfoI	GCGC	5 cut(s)	100, 108, 206, 2635, 4103
Cfr13I	GGNCC	5 cut(s)	1102, 1525, 2453, 2710, 3927
CseI	GACGC	2 cut(s)	162, 2069
Csp6I	GTAC	7 cut(s)	209, 485, 1112, 1238, 2207, 2470, 2686
CspCI	CAANNNNNGTGG	2 cut(s)	3495, 3530
CviQI	GTAC	7 cut(s)	209, 485, 1112, 1238, 2207, 2470, 2686
DraI	TTTAAA	1 cut(s)	2118
DrdI	GACNNNNNNGTC	1 cut(s)	4084
DseDI	GACNNNNNNGTC	1 cut(s)	4084
EaeI	YGGCCR	2 cut(s)	165, 2336
Eam1104I	CTCTTC	2 cut(s)	434, 4011
EarI	CTCTTC	2 cut(s)	434, 4011
EciI	GGCGGA	1 cut(s)	1881
Ecl136II	GAGCTC	2 cut(s)	446, 781
Eco130I	CCWWGG	4 cut(s)	168, 1405, 2689, 3557
Eco147I	AGGCCT	1 cut(s)	3987
Eco24I	GRGCYC	3 cut(s)	448, 783, 1192
Eco31I	GGTCTC	1 cut(s)	337
Eco32I	GATATC	2 cut(s)	618, 1162
Eco47I	GGWCC	3 cut(s)	1102, 2710, 3927
Eco53kI	GAGCTC	2 cut(s)	446, 781
Eco57I	CTGAAG	8 cut(s)	657, 2259, 2387, 2757, 3411, 3548, 3634, 3744
Eco72I	CACGTG	1 cut(s)	1682
Eco81I	CCTNAGG	1 cut(s)	3879
Eco91I	GGTNACC	1 cut(s)	3890
EcoICRI	GAGCTC	2 cut(s)	446, 781
EcoNI	CCTNNNNNAGG	1 cut(s)	2303
EcoO109I	RGGNCCY	1 cut(s)	1102
EcoO65I	GGTNACC	1 cut(s)	3890
EcoRI	GAATTC	2 cut(s)	1818, 3339
EcoRII	CCWGG	8 cut(s)	301, 758, 1245, 2245, 2496, 2628, 3562, 3829
EcoRV	GATATC	2 cut(s)	618, 1162
EcoT14I	CCWWGG	4 cut(s)	168, 1405, 2689, 3557
EcoT22I	ATGCAT	2 cut(s)	1545, 3733
EcoT38I	GRGCYC	3 cut(s)	448, 783, 1192
ErhI	CCWWGG	4 cut(s)	168, 1405, 2689, 3557
Esp3I	CGTCTC	1 cut(s)	180
FalI	AAGNNNNNCTT	8 cut(s)	963, 995, 2067, 2099, 2360, 2392, 4014, 4046
FaqI	GGGAC	1 cut(s)	25
FauI	CCCGC	1 cut(s)	2633
FauNDI	CATATG	1 cut(s)	337
FbaI	TGATCA	2 cut(s)	232, 4155
FblI	GTMKAC	3 cut(s)	695, 1778, 2020
FokI	GGATG	6 cut(s)	1472, 1636, 1817, 3188, 3550, 4022
FriOI	GRGCYC	3 cut(s)	448, 783, 1192
FspBI	CTAG	9 cut(s)	813, 1293, 1908, 2489, 2615, 3077, 3167, 3534, 3897
FspI	TGCGCA	1 cut(s)	107
GlaI	GCGC	5 cut(s)	99, 107, 205, 2634, 4102
GsaI	CCCAGC	3 cut(s)	1212, 2454, 3209
GsuI	CTGGAG	3 cut(s)	285, 1535, 1706
HaeII	RGCGCY	1 cut(s)	2636
HaeIII	GGCC	9 cut(s)	167, 863, 1526, 2229, 2338, 2455, 3562, 3912, 3987
HapII	CCGG	3 cut(s)	243, 701, 2456
HgaI	GACGC	2 cut(s)	162, 2069
HhaI	GCGC	5 cut(s)	100, 108, 206, 2635, 4103
Hin1I	GRCGYC	2 cut(s)	173, 3003
Hin6I	GCGC	5 cut(s)	98, 106, 204, 2633, 4101
HinP1I	GCGC	5 cut(s)	98, 106, 204, 2633, 4101
HincII	GTYRAC	1 cut(s)	3498
HindII	GTYRAC	1 cut(s)	3498
HindIII	AAGCTT	2 cut(s)	2699, 3090
HpaII	CCGG	3 cut(s)	243, 701, 2456
HphI	GGTGA	7 cut(s)	356, 1106, 1478, 2960, 3578, 3587, 3968
Hpy99I	CGWCG	2 cut(s)	183, 414
HpyCH4IV	ACGT	7 cut(s)	1671, 1681, 1789, 2017, 3003, 3295, 3835
HpySE526I	ACGT	7 cut(s)	1671, 1681, 1789, 2017, 3003, 3295, 3835
Hsp92I	GRCGYC	2 cut(s)	173, 3003
HspAI	GCGC	5 cut(s)	98, 106, 204, 2633, 4101
Ksp22I	TGATCA	2 cut(s)	232, 4155
LmnI	GCTCC	8 cut(s)	479, 673, 703, 786, 1725, 2329, 3284, 3821
LweI	GCATC	9 cut(s)	280, 799, 1450, 1552, 1576, 1894, 3163, 3528, 4000
MaeI	CTAG	9 cut(s)	813, 1293, 1908, 2489, 2615, 3077, 3167, 3534, 3897
MaeII	ACGT	7 cut(s)	1671, 1681, 1789, 2017, 3003, 3295, 3835
MfeI	CAATTG	1 cut(s)	4113
MflI	RGATCY	5 cut(s)	220, 2140, 2371, 2524, 3961
MhlI	GDGCHC	4 cut(s)	448, 783, 1192, 2182
MlsI	TGGCCA	2 cut(s)	167, 2338
MluNI	TGGCCA	2 cut(s)	167, 2338
MlyI	GAGTC	5 cut(s)	2156, 2660, 3059, 3761, 3866
MmeI	TCCRAC	9 cut(s)	1200, 1765, 2182, 2528, 2839, 3262, 3316, 3753, 3799
Mox20I	TGGCCA	2 cut(s)	167, 2338
Mph1103I	ATGCAT	2 cut(s)	1545, 3733
MroXI	GAANNNNTTC	2 cut(s)	2958, 3381
MscI	TGGCCA	2 cut(s)	167, 2338
MslI	CAYNNNNRTG	4 cut(s)	272, 366, 2133, 2480
Msp20I	TGGCCA	2 cut(s)	167, 2338
MspA1I	CMGCKG	3 cut(s)	103, 1648, 2762
MspI	CCGG	3 cut(s)	243, 701, 2456
MspR9I	CCNGG	9 cut(s)	244, 303, 760, 1247, 2247, 2498, 2630, 3564, 3831
MunI	CAATTG	1 cut(s)	4113
Mva1269I	GAATGC	4 cut(s)	226, 1368, 3558, 3731
MvaI	CCWGG	8 cut(s)	303, 760, 1247, 2247, 2498, 2630, 3564, 3831
MvnI	CGCG	1 cut(s)	4101
NciI	CCSGG	1 cut(s)	244
NcoI	CCATGG	1 cut(s)	168
NdeI	CATATG	1 cut(s)	337
NlaIV	GGNNCC	5 cut(s)	222, 424, 475, 828, 1224
NmuCI	GTSAC	2 cut(s)	1117, 1958
NsbI	TGCGCA	1 cut(s)	107
NsiI	ATGCAT	2 cut(s)	1545, 3733
NspI	RCATGY	4 cut(s)	953, 2276, 2569, 3060
OliI	CACNNNNGTG	1 cut(s)	2133
PasI	CCCWGGG	1 cut(s)	1246
PceI	AGGCCT	1 cut(s)	3987
PciI	ACATGT	3 cut(s)	2272, 2565, 3056
PctI	GAATGC	4 cut(s)	226, 1368, 3558, 3731
PdmI	GAANNNNTTC	2 cut(s)	2958, 3381
PfoI	TCCNGGA	2 cut(s)	2245, 3829
PleI	GAGTC	5 cut(s)	2155, 2659, 3059, 3761, 3866
PmaCI	CACGTG	1 cut(s)	1682
PmlI	CACGTG	1 cut(s)	1682
PpsI	GAGTC	5 cut(s)	2155, 2659, 3059, 3761, 3866
Ppu21I	YACGTR	2 cut(s)	1682, 3296
PpuMI	RGGWCCY	1 cut(s)	1102
PscI	ACATGT	3 cut(s)	2272, 2565, 3056
Psp124BI	GAGCTC	2 cut(s)	448, 783
Psp5II	RGGWCCY	1 cut(s)	1102
Psp6I	CCWGG	8 cut(s)	301, 758, 1245, 2245, 2496, 2628, 3562, 3829
PspCI	CACGTG	1 cut(s)	1682
PspEI	GGTNACC	1 cut(s)	3890
PspFI	CCCAGC	3 cut(s)	1208, 2450, 3205
PspGI	CCWGG	8 cut(s)	301, 758, 1245, 2245, 2496, 2628, 3562, 3829
PspN4I	GGNNCC	5 cut(s)	222, 424, 475, 828, 1224
PspPI	GGNCC	5 cut(s)	1102, 1525, 2453, 2710, 3927
PspPPI	RGGWCCY	1 cut(s)	1102
PstI	CTGCAG	1 cut(s)	2259
PstNI	CAGNNNCTG	5 cut(s)	14, 308, 635, 2332, 2978
PsuI	RGATCY	5 cut(s)	220, 2140, 2371, 2524, 3961
PvuII	CAGCTG	3 cut(s)	103, 1648, 2762
RsaI	GTAC	7 cut(s)	210, 486, 1113, 1239, 2208, 2471, 2687
RsaNI	GTAC	7 cut(s)	209, 485, 1112, 1238, 2207, 2470, 2686
RseI	CAYNNNNRTG	4 cut(s)	272, 366, 2133, 2480
SacI	GAGCTC	2 cut(s)	448, 783
Sau96I	GGNCC	5 cut(s)	1102, 1525, 2453, 2710, 3927
SchI	GAGTC	5 cut(s)	2156, 2660, 3059, 3761, 3866
ScrFI	CCNGG	9 cut(s)	244, 303, 760, 1247, 2247, 2498, 2630, 3564, 3831
SduI	GDGCHC	4 cut(s)	448, 783, 1192, 2182
SfaNI	GCATC	9 cut(s)	280, 799, 1450, 1552, 1576, 1894, 3163, 3528, 4000
SfcI	CTRYAG	4 cut(s)	522, 963, 1084, 2255
SinI	GGWCC	3 cut(s)	1102, 2710, 3927
SmiMI	CAYNNNNRTG	4 cut(s)	272, 366, 2133, 2480
SmlI	CTYRAG	2 cut(s)	2144, 2315
SmoI	CTYRAG	2 cut(s)	2144, 2315
SseBI	AGGCCT	1 cut(s)	3987
SspI	AATATT	3 cut(s)	1729, 3073, 3301
SspMI	CTAG	9 cut(s)	813, 1293, 1908, 2489, 2615, 3077, 3167, 3534, 3897
SstI	GAGCTC	2 cut(s)	448, 783
StuI	AGGCCT	1 cut(s)	3987
StyD4I	CCNGG	9 cut(s)	242, 301, 758, 1245, 2245, 2496, 2628, 3562, 3829
StyI	CCWWGG	4 cut(s)	168, 1405, 2689, 3557
TaiI	ACGT	7 cut(s)	1674, 1684, 1792, 2020, 3006, 3298, 3838
TaqI	TCGA	7 cut(s)	72, 178, 1753, 1822, 2788, 3478, 4146
TatI	WGTACW	4 cut(s)	208, 1237, 2206, 2469
TauI	GCSGC	1 cut(s)	866
TscAI	CASTG	3 cut(s)	2182, 2896, 4003
TseFI	GTSAC	2 cut(s)	1117, 1958
Tsp45I	GTSAC	2 cut(s)	1117, 1958
TspGWI	ACGGA	3 cut(s)	233, 472, 1054
TspRI	CASTG	3 cut(s)	2182, 2896, 4003
VpaK11BI	GGWCC	3 cut(s)	1102, 2710, 3927
XagI	CCTNNNNNAGG	1 cut(s)	2303
XapI	RAATTY	6 cut(s)	131, 651, 1818, 2958, 3339, 3426
XbaI	TCTAGA	1 cut(s)	2488
XceI	RCATGY	4 cut(s)	953, 2276, 2569, 3060
XmiI	GTMKAC	3 cut(s)	695, 1778, 2020
XmnI	GAANNNNTTC	2 cut(s)	2958, 3381
XspI	CTAG	9 cut(s)	813, 1293, 1908, 2489, 2615, 3077, 3167, 3534, 3897
ZraI	GACGTC	1 cut(s)	3004
Zsp2I	ATGCAT	2 cut(s)	1545, 3733

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.