Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0005249

Physical Location & Seq

Genomic Coordinates Forward (+)

63435591 .. 63448629

Gene Structure 13039 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG321900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4224 bp

ATGAGCAACTCGGTGAGCCATAATTTTCTCCAGCAGAATCCGCTTCAGAGCAAAATCAATTCTTCAGGCATTCCTGCCAACACATTGTTACAACCCAAGTGTATACATCCAGTGCCAGCGCAGCCGCGCAAGTCTTGTTCTATATCCAAGAAATTTTGTGGCAACAGTTTGCAGAAGCCGAAGCTGGCCATGGGGTCTCGTCGGCCTGTTGCATTTGTTCCTTGCGCTGTACTAACCACGGATCCGCATTCTGATCAGCTTGCCGGGAAATACAACCTTGATGGTAACATTGAATTGCAGGTTTATGTTGATGCTTCCTCTCCAGGGTCTGCCACTAAAGTAGAATTTCAGGTCTCATATGGCAGTGATTCTTTGGTTCTTCATTGGGGTGGGATAAAAGATAGAAAAGAAAAATGGGTGCTTCCTTCTCGTCGCCCAGATGGAACCAAAGAATATAAGAAGAGAGCTCTTAGAACTCCTTTTGTGAAATCTGGCTCCAATTCCGTACTTAAAATTGAGATTGATGATCCTGCAATACAAGCTATAGAGTTTCTCATAGTTGATGAAAGTCAGAACAGATGGTTTAAGAACAATGGCGGCAACTTTCATGTTAAATTACCTTCGAAAGAGAAGTTGATATCAAATGTTTCAGTTCCTGAAGATTTAGTGCAAATTCAAGCATATCTGAGGTGGGAGCGAAAGGGTAAACAGATGTACACACCAGAGCAAGAGAAGGTGGAGTATGAAGCGGCCCGAACTGAGCTATTGGAGGAAGTAGCCAGGGGTGCTTCCATACAAGAGCTCCAAGCAAGGCTGACTAAGAAAACTGATGCTAGTAACAGTCAGGAACCATCTCATGCTGATAACAAAGAAGTTTCCAAGGCCGCAAAGAAACATGAGAGTAAAAAGGTTTCCAGAACTGAAAGAATTAAGCGGAAGCAACGAGATTTAATGAAGATTCTCAACAAACATGCTACTAAACCTGTAGATGAAGCTAAACTTGCGCATGAAGAATCAAGGAAACCAAAAGCCTTGAAAGCAGTTGAGCTATTTGCCAAGGCAAAAGAAGAACAAGATGGTGCTTCCGTCCTGAACAAAAACATCTACAGGCTCGGTGATAAGGACCTGCTGGTACTTGTGACAAAGGTTGCTGATAAGACAAGGGTTCATCTGGCAACAGATATCAAAGAGCCAATTAACATTCATTGGGCTCTATCTGTAAACAAAGCTGGAGAGTGGTTGGAACCACCTCCAAATGTACTGCCCCAGGGTTCAGTTTCTCTAAATGGGGCTGTTGAAACACAATTTGTATCTAGTTCTGTTGATTCTACTTATGAGGTTCAATCTTTAGAAATAGAAATGCAAGAGGAAAGTTTCAAAGGAATGCCATTCGTCCTTTGTTCTTCTGGAAACTGGATTAAGAACCAAGGATCAGATTTCTACGCTGACTTTGGTGTTGCGGTCAAGAAAGTTCAGAAGGATGCTGGTGATGGAAAAGGTACTGCAAAGGATTTATTAGATAAAATAGCAGGTCTGGAGAGTGAGGCGCAAAAATCCTTTATGCATCGGTTTAACATTGCAGCAGACTTGATAAATCAAGCAAAAGATGCTGGCAAATTAGGTCTTGCAGGTATTCTGGTGTGGATGAGGTTTATGGCTATGAGGCAGCTGATTTGGAATAAAAATTACAATGTCAAACCACGTGAGATTAGTAAAGCACAGGACAGGCTCACAGGCTTGCTCCAGAATATTTACACAAGTCATCCACAGTATCGAGAGCTTCTACGGTTGATATTGTCTACTGTTGGACGTGGAGGAGAAGGGGATGTGGGACAGCGAATTCGAGATGAGATCCTTGTTATCCAGAGAAACTGTGATTGCAAGGGCGGAATGATGGAGGAATGGCATCAAAAGTTGCATAATAACACTAGCCCAGATGATGTTGTTATATGTCAGGCGCTAATTGATTACATCAAAAGTGACTTTGATATTGGTGTTTACTGGAAAACTTTGAACGAGAATGGAATTACAAAAGAACGTCTTCTAAGTTATGACCGTGCAATCCACAATGAACCAAGTTTCAGGGCAGATCAGAAAGAGCGTCTTCTGCATGATCTGGGGAACTACATGAGAACTTTAAAGGCAGTTCACTCAGGTGCAGATCTTGAGTCTGCCATCACAAATTGTTTGGGTTACAGTGCTCAGGGTCAAGGCTTCATGGTTGGAGTACAAATAAATCCTGTATCTGGCCTGCCCTCAGAAGTTCCAGGACTGCTGCAATTTGTAATGGAACATGTTGAGGACAAGAATGTGGAAGTCCTTATTGAGGGTTTGCTTGAGGCTCGTCAGGAGCTGTGGCCATTGCTCTCTAAGCCTAATGATCGCCTAAAGGATCTCTTATTTTTGGACATTGCCCTTGATTCTACTGTGAGAACCGCCATTGAGAGGGGATATGAAGAACTGAATAATGCTGGGCCGGAGAAAATTATGTACTTCATCTCAATGGTTCTAGAAAACCTGGCATTATCATCAGATGATAATGAGGATCTCATGTACTGTTTGAAGGGATGGAATCATGCGTTAAACATGTTAAAGAGTAATACCGATAACTGGGCATTATATGCTAAATCAATCCTAGACAGAACCCGCCTGGCGCTTGCAAACAAGGCAGAATCGTATCACAGTATTTTACAGCCATCAGCAGAGTACCTTGGATCAAAGCTTGGAGTGGACCAACGGGCTTTGAGCATATTTACTGAAGAAGTTATCCGTGCTGGATCAGCTGCCTCTTTGTCCGCACTCGTTAATCGACTTGATCCAGTTCTTCGGAAAACTGCTAATTTGGGAAGTTGGCAGGTTATCAGCCCAGTTGAAGTGGTTGGATATGTTGTAGTTGTGGACGAGTTGCTCACTGTCCAGAATAAAGTATATGAGAAGCCGACTATTTTGGTGGCAAGAAATGTGAGAGGTGAGGAAGAAATTCCAGATGGCACAGTTGCTGTGCTAACACCTGATATGCCAGACGTCCTATCTCATGTTTCTGTCCGAGCAAGAAATAGCAAGGTTTGTTTTGCGACATGTTTTGACTCCAATATTCTAGCTGACCTCCAAGCTTGTAAAGGGAAGTTATTGCGTGTAAAGCCAACTACTGCTGATGTAGTTTATAGCGAGGTTAATGAGAGTGAGCTAGGAGATGCAAGTTCAACTAATTCAAATGAAGACATCCCAGCTCTGACATTAGTGAAGAAACAGTTTACGGGTAGATATGCCATATCATCTGATGAGTTCACAAGTGAAATGGTTGGAGCTAAATCACGTAATATTTCTTATATAAAAGGAAAACTACCCTCTTGGGTTGGAATTCCTACATCAGTTGCCCTACCATTTGGAGTATTTGAAAAAGTTCTTTCTGAAGATTCAAATAAGGCTGTTGCTGACAAGTTAGAAATTCTAAAGAAAAAGTTAAAAGAAGGAGATTTTGGTTCACTTGGGGAGATTCGAGAGACAGTTTTACAGTTGACAGCACCACCCCCGTTGGTGCAAGAGCTGAAGACTAAGATGCAAAGTTCAGGAATGCCTTGGCCTGGTGATGAGGGTGAACAACGATGGGAACAAGCATGGTTGGCTATATTAAAGGTCTGGGCTTCAAAGTGGAATGAGAGAGCATACTTCAGCACAAGAAAAGTGAAATTAGACCATGATTATCTTTGCATGGCTGTCCTTGTTCAAGAAATAATAAATGCCGACTATGCATTCGTTATTCACACAACTAACCCATCTTCAGGAGACTCGTCGGAGATATATGCTGAGGTTGTCAAAGGTCTTGGAGAAACCTTAGTTGGAGCTTATCCTGGACGTGCTTTAAGTTTTATTAGCAAGAAAAATGCTCTGGACTCTCCTCAGGTGTTAGGTTACCCTAGCAAACCGATTGGCCTCTTTATCAGACAGTCCATAATCTTCCGATCCGATTCCAATGGTGAAGATCTTGAAGGTTATGCTGGTGCAGGCCTTTATGACAGTGTGCCTATGGATGCGGAAGAGGAAGTCGTGCTTGACTACTCATCTGACCCACTCGTTACTGATGGGAACTTCCAAAAGACAATTCTGTCCAGCATTGCTCGCGCAGGAAGTGCAATCGAGGAGTTATATGGTTCAGCACAAGACATTGAAGGTGTGATCAGAGATGGGAAACTCTATGTTGTCCAGACAAGACCCCAGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009628 GO:0009631 GO:0009941 GO:0009987 GO:0016052 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016781 GO:0031967 GO:0031975 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050521 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPDK_N

Protein Analysis

1407

Amino Acids

156.38

Weight (kDa)

6.27

Isoelectric Point (pI)

42.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ig_N_CWD1	PF23166	89 - 206	1.3e-41	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
DUF7067	PF23229	220 - 275	4.1e-28	Alpha-glucan water dikinase, DUF7067
Ig_N_CWD1	PF23166	366 - 483	1.7e-26	Alpha-glucan water dikinase 1 Ig-like N-terminal domain
GWD1_pHisD	PF22973	950 - 1062	2.5e-53	Alpha-glucan water dikinase, phosphohistidine-like domain
PPDK_N	PF01326	1205 - 1281	4.5e-12	Pyruvate phosphate dikinase, AMP/ATP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0005249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10760 AT1G10760 AT1G10760
fragaria_vesca	FvH4_4g25800 FvH4_4g25800 FvH4_4g25800 FvH4_4g25800
malus_domestica	MD13G1096800.v1.1 MD16G1097800.v1.1
prunus_persica	Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1 Prupe.1G248900_v2.0.a1
pyrus_communis	pycom13g08450 pycom16g08330
rosa_chinensis	RchiOBHm_Chr4g0433251
rosa_laevigata	RLG00000006771
rosa_multiflora	Rmu_co8055492.1_g000001 Rmu_sc0010643.1_g000001
rosa_roxburghii	Rroxscaffold_5G00374810
rosa_rugosa	Rorug04G0267200 Rorug04G0267300 Rorug04G0267400 Rorug04G0267400
rosa_samantha	Rh4AG321900 Rh4BG329300 Rh4CG344600
rosa_wichuraiana	Rw4G027860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	4105
AatII	GACGTC	1 cut(s)	3027
Acc16I	TGCGCA	1 cut(s)	1005
Acc36I	ACCTGC	5 cut(s)	289, 1134, 1520, 1619, 2845
AccI	GTMKAC	2 cut(s)	103, 1799
AccII	CGCG	2 cut(s)	127, 4122
AcoI	YGGCCR	2 cut(s)	186, 2357
AcsI	RAATTY	7 cut(s)	152, 344, 672, 1839, 2979, 3360, 3447
AcuI	CTGAAG	8 cut(s)	29, 48, 678, 2778, 3432, 3569, 3655, 3765
AcvI	CACGTG	1 cut(s)	1703
AcyI	GRCGYC	1 cut(s)	3024
AfiI	CCNNNNNNNGG	7 cut(s)	324, 2246, 2326, 2445, 2610, 3584, 3782
AflIII	ACRYGT	3 cut(s)	2293, 2586, 3077
AjiI	CACGTC	2 cut(s)	1811, 3857
AjnI	CCWGG	8 cut(s)	322, 779, 1266, 2266, 2517, 2649, 3583, 3850
AjuI	GAANNNNNNNTTGG	6 cut(s)	3462, 3494, 3822, 3854, 4086, 4118
AleI	CACNNNNGTG	1 cut(s)	2154
Alw21I	GWGCWC	3 cut(s)	469, 804, 2203
Alw26I	GTCTC	4 cut(s)	201, 358, 3497, 3780
AlwNI	CAGNNNCTG	4 cut(s)	329, 656, 2353, 2999
ApeKI	GCWGC	5 cut(s)	121, 1580, 1666, 2275, 2783
ApoI	RAATTY	7 cut(s)	152, 344, 672, 1839, 2979, 3360, 3447
ArsI	GACNNNNNNTTYG	4 cut(s)	1824, 1856, 3737, 3769
Asp700I	GAANNNNTTC	3 cut(s)	2040, 2979, 3402
AspLEI	GCGC	8 cut(s)	121, 129, 227, 1006, 1549, 1960, 2656, 4124
AspS9I	GGNCC	4 cut(s)	751, 1123, 2474, 2731
AsuC2I	CCSGG	1 cut(s)	265
AsuHPI	GGTGA	7 cut(s)	25, 1127, 1499, 2981, 3599, 3608, 3989
AsuII	TTCGAA	1 cut(s)	623
AvaII	GGWCC	2 cut(s)	1123, 2731
AxyI	CCTNAGG	1 cut(s)	3900
BalI	TGGCCA	2 cut(s)	188, 2359
BamHI	GGATCC	1 cut(s)	241
BanII	GRGCYC	3 cut(s)	469, 804, 1213
BbrPI	CACGTG	1 cut(s)	1703
BbsI	GAAGAC	4 cut(s)	2033, 2096, 3225, 3557
Bbv12I	GWGCWC	3 cut(s)	469, 804, 2203
BbvCI	CCTCAGC	1 cut(s)	3807
BbvI	GCAGC	5 cut(s)	133, 1592, 1678, 2262, 2770
BciT130I	CCWGG	8 cut(s)	324, 781, 1268, 2268, 2519, 2651, 3585, 3852
BclI	TGATCA	2 cut(s)	253, 4176
BcnI	CCSGG	1 cut(s)	265
BcoDI	GTCTC	4 cut(s)	201, 358, 3497, 3780
BfaI	CTAG	8 cut(s)	834, 1314, 1929, 2510, 2636, 3098, 3188, 3918
BfmI	CTRYAG	3 cut(s)	543, 984, 1105
BfoI	RGCGCY	2 cut(s)	1961, 2657
BfuAI	ACCTGC	5 cut(s)	289, 1134, 1520, 1619, 2845
BglII	AGATCT	2 cut(s)	2161, 3982
BisI	GCNGC	9 cut(s)	122, 125, 598, 750, 885, 1581, 1667, 2276, 2784
BlsI	GCNGC	9 cut(s)	123, 126, 599, 751, 886, 1582, 1668, 2277, 2785
Bme1390I	CCNGG	9 cut(s)	265, 324, 781, 1268, 2268, 2519, 2651, 3585, 3852
Bme18I	GGWCC	2 cut(s)	1123, 2731
BmgBI	CACGTC	2 cut(s)	1811, 3857
BmgT120I	GGNCC	4 cut(s)	751, 1123, 2474, 2731
BmiI	GGNNCC	5 cut(s)	243, 445, 496, 849, 1245
BmrFI	CCNGG	9 cut(s)	265, 324, 781, 1268, 2268, 2519, 2651, 3585, 3852
BmrI	ACTGGG	2 cut(s)	2620, 2861
BmsI	GCATC	9 cut(s)	301, 820, 1471, 1573, 1597, 1915, 3184, 3549, 4021
BmuI	ACTGGG	2 cut(s)	2620, 2861
BpiI	GAAGAC	4 cut(s)	2033, 2096, 3225, 3557
BpmI	CTGGAG	5 cut(s)	14, 306, 1251, 1556, 1727
Bpu10I	CCTNAGC	2 cut(s)	2202, 3807
Bpu14I	TTCGAA	1 cut(s)	623
BpuEI	CTTGAG	2 cut(s)	2186, 2357
BpuMI	CCSGG	1 cut(s)	265
BsaAI	YACGTR	2 cut(s)	1703, 3317
BsaHI	GRCGYC	1 cut(s)	3024
BsaI	GGTCTC	2 cut(s)	201, 358
BsaXI	ACNNNNNCTCC	2 cut(s)	786, 816
Bsc4I	CCNNNNNNNGG	7 cut(s)	324, 2246, 2326, 2445, 2610, 3584, 3782
Bse1I	ACTGG	6 cut(s)	110, 1418, 2006, 2615, 2819, 2867
Bse21I	CCTNAGG	1 cut(s)	3900
Bse3DI	GCAATG	4 cut(s)	1575, 2360, 2409, 4113
BseBI	CCWGG	8 cut(s)	324, 781, 1268, 2268, 2519, 2651, 3585, 3852
BseGI	GGATG	8 cut(s)	106, 1486, 1650, 1762, 1831, 2573, 3222, 4036
BseLI	CCNNNNNNNGG	7 cut(s)	324, 2246, 2326, 2445, 2610, 3584, 3782
BseMI	GCAATG	4 cut(s)	1575, 2360, 2409, 4113
BseMII	CTCAG	7 cut(s)	677, 750, 2166, 2216, 2271, 3798, 3914
BseNI	ACTGG	6 cut(s)	110, 1418, 2006, 2615, 2819, 2867
BseRI	GAGGAG	3 cut(s)	1830, 3888, 4154
BseXI	GCAGC	5 cut(s)	133, 1592, 1678, 2262, 2770
BseYI	CCCAGC	2 cut(s)	2471, 3226
BsgI	GTGCAG	2 cut(s)	2178, 4023
Bsh1236I	CGCG	2 cut(s)	127, 4122
BsiHKAI	GWGCWC	3 cut(s)	469, 804, 2203
BsiSI	CCGG	2 cut(s)	264, 2477
BslFI	GGGAC	1 cut(s)	1845
BslI	CCNNNNNNNGG	7 cut(s)	324, 2246, 2326, 2445, 2610, 3584, 3782
BsmAI	GTCTC	4 cut(s)	201, 358, 3497, 3780
BsmFI	GGGAC	1 cut(s)	1845
BsmI	GAATGC	5 cut(s)	69, 247, 1389, 3579, 3752
Bso31I	GGTCTC	2 cut(s)	201, 358
Bsp119I	TTCGAA	1 cut(s)	623
Bsp1286I	GDGCHC	4 cut(s)	469, 804, 1213, 2203
Bsp1407I	TGTACA	1 cut(s)	714
Bsp19I	CCATGG	1 cut(s)	189
BspCNI	CTCAG	7 cut(s)	678, 751, 2165, 2215, 2270, 3799, 3913
BspFNI	CGCG	2 cut(s)	127, 4122
BspLI	GGNNCC	5 cut(s)	243, 445, 496, 849, 1245
BspMI	ACCTGC	5 cut(s)	289, 1134, 1520, 1619, 2845
BspT104I	TTCGAA	1 cut(s)	623
BspTNI	GGTCTC	2 cut(s)	201, 358
BsrDI	GCAATG	4 cut(s)	1575, 2360, 2409, 4113
BsrGI	TGTACA	1 cut(s)	714
BsrI	ACTGG	6 cut(s)	110, 1418, 2006, 2615, 2819, 2867
BssNAI	GTATAC	1 cut(s)	104
BssNI	GRCGYC	1 cut(s)	3024
BssT1I	CCWWGG	6 cut(s)	189, 879, 1056, 1426, 2710, 3578
Bst1107I	GTATAC	1 cut(s)	104
Bst2UI	CCWGG	8 cut(s)	324, 781, 1268, 2268, 2519, 2651, 3585, 3852
Bst6I	CTCTTC	2 cut(s)	455, 4032
BstACI	GRCGYC	1 cut(s)	3024
BstAPI	GCANNNNNTGC	3 cut(s)	1607, 2621, 4130
BstAUI	TGTACA	1 cut(s)	714
BstBAI	YACGTR	2 cut(s)	1703, 3317
BstBI	TTCGAA	1 cut(s)	623
BstC8I	GCNNGC	9 cut(s)	117, 186, 261, 1612, 1739, 2252, 2658, 4006, 4120
BstDSI	CCRYGG	2 cut(s)	189, 237
BstEII	GGTNACC	1 cut(s)	3911
BstENI	CCTNNNNNAGG	1 cut(s)	2324
BstF5I	GGATG	8 cut(s)	106, 1486, 1650, 1762, 1831, 2573, 3222, 4036
BstFNI	CGCG	2 cut(s)	127, 4122
BstH2I	RGCGCY	2 cut(s)	1961, 2657
BstHHI	GCGC	8 cut(s)	121, 129, 227, 1006, 1549, 1960, 2656, 4124
BstMAI	GTCTC	4 cut(s)	201, 358, 3497, 3780
BstNI	CCWGG	8 cut(s)	324, 781, 1268, 2268, 2519, 2651, 3585, 3852
BstNSI	RCATGY	4 cut(s)	974, 2297, 2590, 3081
BstPI	GGTNACC	1 cut(s)	3911
BstSCI	CCNGG	9 cut(s)	263, 322, 779, 1266, 2266, 2517, 2649, 3583, 3850
BstSFI	CTRYAG	3 cut(s)	543, 984, 1105
BstUI	CGCG	2 cut(s)	127, 4122
BstV1I	GCAGC	5 cut(s)	133, 1592, 1678, 2262, 2770
BstV2I	GAAGAC	4 cut(s)	2033, 2096, 3225, 3557
BstX2I	RGATCY	6 cut(s)	241, 1851, 2161, 2392, 2545, 3982
BstYI	RGATCY	6 cut(s)	241, 1851, 2161, 2392, 2545, 3982
BstZ17I	GTATAC	1 cut(s)	104
Bsu36I	CCTNAGG	1 cut(s)	3900
BtgI	CCRYGG	2 cut(s)	189, 237
BtrI	CACGTC	2 cut(s)	1811, 3857
BtsCI	GGATG	8 cut(s)	106, 1486, 1650, 1762, 1831, 2573, 3222, 4036
BtsI	GCAGTG	1 cut(s)	370
BtsIMutI	CAGTG	5 cut(s)	117, 370, 2203, 2910, 4024
BveI	ACCTGC	5 cut(s)	289, 1134, 1520, 1619, 2845
Cac8I	GCNNGC	9 cut(s)	117, 186, 261, 1612, 1739, 2252, 2658, 4006, 4120
CaiI	CAGNNNCTG	4 cut(s)	329, 656, 2353, 2999
CfoI	GCGC	8 cut(s)	121, 129, 227, 1006, 1549, 1960, 2656, 4124
Cfr13I	GGNCC	4 cut(s)	751, 1123, 2474, 2731
CseI	GACGC	1 cut(s)	2090
CspCI	CAANNNNNGTGG	2 cut(s)	3516, 3551
DraI	TTTAAA	1 cut(s)	2139
DrdI	GACNNNNNNGTC	1 cut(s)	4105
DseDI	GACNNNNNNGTC	1 cut(s)	4105
EaeI	YGGCCR	2 cut(s)	186, 2357
Eam1104I	CTCTTC	2 cut(s)	455, 4032
EarI	CTCTTC	2 cut(s)	455, 4032
EciI	GGCGGA	1 cut(s)	1902
Ecl136II	GAGCTC	2 cut(s)	467, 802
Eco130I	CCWWGG	6 cut(s)	189, 879, 1056, 1426, 2710, 3578
Eco147I	AGGCCT	1 cut(s)	4008
Eco24I	GRGCYC	3 cut(s)	469, 804, 1213
Eco31I	GGTCTC	2 cut(s)	201, 358
Eco32I	GATATC	2 cut(s)	639, 1183
Eco47I	GGWCC	2 cut(s)	1123, 2731
Eco53kI	GAGCTC	2 cut(s)	467, 802
Eco57I	CTGAAG	8 cut(s)	29, 48, 678, 2778, 3432, 3569, 3655, 3765
Eco72I	CACGTG	1 cut(s)	1703
Eco81I	CCTNAGG	1 cut(s)	3900
Eco91I	GGTNACC	1 cut(s)	3911
EcoICRI	GAGCTC	2 cut(s)	467, 802
EcoNI	CCTNNNNNAGG	1 cut(s)	2324
EcoO109I	RGGNCCY	1 cut(s)	1123
EcoO65I	GGTNACC	1 cut(s)	3911
EcoRI	GAATTC	2 cut(s)	1839, 3360
EcoRII	CCWGG	8 cut(s)	322, 779, 1266, 2266, 2517, 2649, 3583, 3850
EcoRV	GATATC	2 cut(s)	639, 1183
EcoT14I	CCWWGG	6 cut(s)	189, 879, 1056, 1426, 2710, 3578
EcoT22I	ATGCAT	2 cut(s)	1566, 3754
EcoT38I	GRGCYC	3 cut(s)	469, 804, 1213
ErhI	CCWWGG	6 cut(s)	189, 879, 1056, 1426, 2710, 3578
FaqI	GGGAC	1 cut(s)	1845
FauI	CCCGC	1 cut(s)	2654
FauNDI	CATATG	1 cut(s)	358
FbaI	TGATCA	2 cut(s)	253, 4176
FblI	GTMKAC	2 cut(s)	103, 1799
Fnu4HI	GCNGC	9 cut(s)	122, 125, 598, 750, 885, 1581, 1667, 2276, 2784
FokI	GGATG	8 cut(s)	93, 1493, 1657, 1749, 1838, 2580, 3209, 4043
FriOI	GRGCYC	3 cut(s)	469, 804, 1213
Fsp4HI	GCNGC	9 cut(s)	122, 125, 598, 750, 885, 1581, 1667, 2276, 2784
FspBI	CTAG	8 cut(s)	834, 1314, 1929, 2510, 2636, 3098, 3188, 3918
FspI	TGCGCA	1 cut(s)	1005
GlaI	GCGC	8 cut(s)	120, 128, 226, 1005, 1548, 1959, 2655, 4123
GluI	GCNGC	9 cut(s)	122, 125, 598, 750, 885, 1581, 1667, 2276, 2784
GsaI	CCCAGC	2 cut(s)	2475, 3230
GsuI	CTGGAG	5 cut(s)	14, 306, 1251, 1556, 1727
HaeII	RGCGCY	2 cut(s)	1961, 2657
HapII	CCGG	2 cut(s)	264, 2477
HgaI	GACGC	1 cut(s)	2090
HhaI	GCGC	8 cut(s)	121, 129, 227, 1006, 1549, 1960, 2656, 4124
Hin1I	GRCGYC	1 cut(s)	3024
Hin6I	GCGC	8 cut(s)	119, 127, 225, 1004, 1547, 1958, 2654, 4122
HinP1I	GCGC	8 cut(s)	119, 127, 225, 1004, 1547, 1958, 2654, 4122
HincII	GTYRAC	1 cut(s)	3519
HindII	GTYRAC	1 cut(s)	3519
HindIII	AAGCTT	2 cut(s)	2720, 3111
HpaII	CCGG	2 cut(s)	264, 2477
HphI	GGTGA	7 cut(s)	25, 1127, 1499, 2981, 3599, 3608, 3989
Hpy99I	CGWCG	3 cut(s)	204, 435, 3796
HpyAV	CCTTC	9 cut(s)	435, 630, 727, 1471, 1814, 2557, 3464, 3983, 4163
HpyCH4IV	ACGT	6 cut(s)	1702, 1810, 2038, 3024, 3316, 3856
HpySE526I	ACGT	6 cut(s)	1702, 1810, 2038, 3024, 3316, 3856
Hsp92I	GRCGYC	1 cut(s)	3024
HspAI	GCGC	8 cut(s)	119, 127, 225, 1004, 1547, 1958, 2654, 4122
Ksp22I	TGATCA	2 cut(s)	253, 4176
LmnI	GCTCC	7 cut(s)	500, 694, 807, 1746, 2350, 3305, 3842
Lsp1109I	GCAGC	5 cut(s)	133, 1592, 1678, 2262, 2770
LweI	GCATC	9 cut(s)	301, 820, 1471, 1573, 1597, 1915, 3184, 3549, 4021
MaeI	CTAG	8 cut(s)	834, 1314, 1929, 2510, 2636, 3098, 3188, 3918
MaeII	ACGT	6 cut(s)	1702, 1810, 2038, 3024, 3316, 3856
MaeIII	GTNAC	8 cut(s)	87, 284, 836, 1138, 1979, 2192, 3911, 4075
MflI	RGATCY	6 cut(s)	241, 1851, 2161, 2392, 2545, 3982
MhlI	GDGCHC	4 cut(s)	469, 804, 1213, 2203
MlsI	TGGCCA	2 cut(s)	188, 2359
MluNI	TGGCCA	2 cut(s)	188, 2359
MlyI	GAGTC	4 cut(s)	2177, 3080, 3782, 3887
MmeI	TCCRAC	8 cut(s)	1221, 1786, 2203, 2860, 3283, 3337, 3774, 3820
Mox20I	TGGCCA	2 cut(s)	188, 2359
Mph1103I	ATGCAT	2 cut(s)	1566, 3754
MroXI	GAANNNNTTC	3 cut(s)	2040, 2979, 3402
MscI	TGGCCA	2 cut(s)	188, 2359
MslI	CAYNNNNRTG	3 cut(s)	387, 2154, 2501
Msp20I	TGGCCA	2 cut(s)	188, 2359
MspA1I	CMGCKG	2 cut(s)	1669, 2783
MspI	CCGG	2 cut(s)	264, 2477
MspR9I	CCNGG	9 cut(s)	265, 324, 781, 1268, 2268, 2519, 2651, 3585, 3852
Mva1269I	GAATGC	5 cut(s)	69, 247, 1389, 3579, 3752
MvaI	CCWGG	8 cut(s)	324, 781, 1268, 2268, 2519, 2651, 3585, 3852
MvnI	CGCG	2 cut(s)	127, 4122
NciI	CCSGG	1 cut(s)	265
NcoI	CCATGG	1 cut(s)	189
NdeI	CATATG	1 cut(s)	358
NlaIV	GGNNCC	5 cut(s)	243, 445, 496, 849, 1245
NmuCI	GTSAC	2 cut(s)	1138, 1979
NsbI	TGCGCA	1 cut(s)	1005
NsiI	ATGCAT	2 cut(s)	1566, 3754
NspI	RCATGY	4 cut(s)	974, 2297, 2590, 3081
NspV	TTCGAA	1 cut(s)	623
OliI	CACNNNNGTG	1 cut(s)	2154
PasI	CCCWGGG	1 cut(s)	1267
PceI	AGGCCT	1 cut(s)	4008
PciI	ACATGT	3 cut(s)	2293, 2586, 3077
PctI	GAATGC	5 cut(s)	69, 247, 1389, 3579, 3752
PdmI	GAANNNNTTC	3 cut(s)	2040, 2979, 3402
PfoI	TCCNGGA	2 cut(s)	2266, 3850
PkrI	GCNGC	9 cut(s)	123, 126, 599, 751, 886, 1582, 1668, 2277, 2785
PleI	GAGTC	4 cut(s)	2176, 3080, 3782, 3887
PmaCI	CACGTG	1 cut(s)	1703
PmlI	CACGTG	1 cut(s)	1703
PpsI	GAGTC	4 cut(s)	2176, 3080, 3782, 3887
Ppu21I	YACGTR	2 cut(s)	1703, 3317
PpuMI	RGGWCCY	1 cut(s)	1123
PscI	ACATGT	3 cut(s)	2293, 2586, 3077
Psp124BI	GAGCTC	2 cut(s)	469, 804
Psp5II	RGGWCCY	1 cut(s)	1123
Psp6I	CCWGG	8 cut(s)	322, 779, 1266, 2266, 2517, 2649, 3583, 3850
PspCI	CACGTG	1 cut(s)	1703
PspEI	GGTNACC	1 cut(s)	3911
PspFI	CCCAGC	2 cut(s)	2471, 3226
PspGI	CCWGG	8 cut(s)	322, 779, 1266, 2266, 2517, 2649, 3583, 3850
PspN4I	GGNNCC	5 cut(s)	243, 445, 496, 849, 1245
PspPI	GGNCC	4 cut(s)	751, 1123, 2474, 2731
PspPPI	RGGWCCY	1 cut(s)	1123
PstNI	CAGNNNCTG	4 cut(s)	329, 656, 2353, 2999
PsuI	RGATCY	6 cut(s)	241, 1851, 2161, 2392, 2545, 3982
PvuII	CAGCTG	2 cut(s)	1669, 2783
RseI	CAYNNNNRTG	3 cut(s)	387, 2154, 2501
SacI	GAGCTC	2 cut(s)	469, 804
SatI	GCNGC	9 cut(s)	122, 125, 598, 750, 885, 1581, 1667, 2276, 2784
Sau96I	GGNCC	4 cut(s)	751, 1123, 2474, 2731
SchI	GAGTC	4 cut(s)	2177, 3080, 3782, 3887
ScrFI	CCNGG	9 cut(s)	265, 324, 781, 1268, 2268, 2519, 2651, 3585, 3852
SduI	GDGCHC	4 cut(s)	469, 804, 1213, 2203
SfaNI	GCATC	9 cut(s)	301, 820, 1471, 1573, 1597, 1915, 3184, 3549, 4021
SfcI	CTRYAG	3 cut(s)	543, 984, 1105
SfuI	TTCGAA	1 cut(s)	623
SinI	GGWCC	2 cut(s)	1123, 2731
SmiMI	CAYNNNNRTG	3 cut(s)	387, 2154, 2501
SmlI	CTYRAG	2 cut(s)	2165, 2336
SmoI	CTYRAG	2 cut(s)	2165, 2336
SseBI	AGGCCT	1 cut(s)	4008
SspI	AATATT	3 cut(s)	1750, 3094, 3322
SspMI	CTAG	8 cut(s)	834, 1314, 1929, 2510, 2636, 3098, 3188, 3918
SstI	GAGCTC	2 cut(s)	469, 804
StuI	AGGCCT	1 cut(s)	4008
StyD4I	CCNGG	9 cut(s)	263, 322, 779, 1266, 2266, 2517, 2649, 3583, 3850
StyI	CCWWGG	6 cut(s)	189, 879, 1056, 1426, 2710, 3578
TaiI	ACGT	6 cut(s)	1705, 1813, 2041, 3027, 3319, 3859
TaqI	TCGA	6 cut(s)	623, 1774, 1843, 2809, 3499, 4137
TatI	WGTACW	6 cut(s)	229, 714, 1258, 2227, 2490, 2553
TauI	GCSGC	4 cut(s)	127, 600, 752, 887
TscAI	CASTG	5 cut(s)	117, 370, 2203, 2917, 4024
TseFI	GTSAC	2 cut(s)	1138, 1979
TseI	GCWGC	5 cut(s)	121, 1580, 1666, 2275, 2783
Tsp45I	GTSAC	2 cut(s)	1138, 1979
TspGWI	ACGGA	4 cut(s)	254, 493, 1075, 2759
TspRI	CASTG	5 cut(s)	117, 370, 2203, 2917, 4024
VpaK11BI	GGWCC	2 cut(s)	1123, 2731
XagI	CCTNNNNNAGG	1 cut(s)	2324
XapI	RAATTY	7 cut(s)	152, 344, 672, 1839, 2979, 3360, 3447
XbaI	TCTAGA	1 cut(s)	2509
XceI	RCATGY	4 cut(s)	974, 2297, 2590, 3081
XmiI	GTMKAC	2 cut(s)	103, 1799
XmnI	GAANNNNTTC	3 cut(s)	2040, 2979, 3402
XspI	CTAG	8 cut(s)	834, 1314, 1929, 2510, 2636, 3098, 3188, 3918
ZraI	GACGTC	1 cut(s)	3025
Zsp2I	ATGCAT	2 cut(s)	1566, 3754

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.