FvH4_4g29240

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004856
Physical Location & Seq
Reverse (-)
29451980 .. 29454517
2538 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g29240.t1

Sequence Viewer

Length: 1512 bp
ATGCATTTGCAATGGAGGCCTATAGATCTGTTGGGAGGGAGCCTTTACTCCCACACTCAATGCCAGAGTCCGAAAAAAAATTGCGTGTTGCACCATAGATATTTGCGTGCAATTCTGCATATAGCAATATGCATGTCGAAATTGTACCTCTCTTTTCTCACGATAATCACTCTTCTCTTCTGCATAAAAGCCTTTCAAATGTTTTTCCTTGTCAATAATCATATCGTTGATCATCATCACCAACAACATCATATATCTTTCTCTTCTTCTTCACCATCTCATTTTCAAGGGGACTACGTGAACAATGGATTTCTGATGGTCTCATCCAATGGAGGATTGAATCAAATGCGAGCTGGGATTTGTGACATGGTGGCTATTGCAAGGTTTTTAAATGTCACGCTTATAGTTCCTGAGTTGGATAAAACCTCCTTTTGGAATGATCAGAGTCAGTTTGATGATATATTCGACGTCGATCACTTCATAGCATCATTGAGCAATGAAGTCCGGATATTGAAAGTTCTGCCCCAGAAACAGAGAAGAAGAATAGAGATGGCTTCCCTCTATTCCATGCCTCCTGTTAGTTGGTCTAATATGACGTTTTATTACAACACGATTCTTCCTCGGATAAAGAAGTATGAAGTCCTGCATTTCACTAAAACTGATACAAGACTTGCCAATAATGGGATCCCTGTAGAGGTTCAGAAAATGCGCTGCAAGGCAAACTATGAAGCCCTGAGGTTCACTCCCCCAATTGAGGAACTGGGAAAAAAGATTGTAAGGCTTATGAGAGAAAGGGGGCCATTCTTAGTTCTCCATCTCAGATATGAAATGGACATGCTAGCATTTTCAGGTTGTACTGAAGGCTGCGATGACGATGAAATTGAAGAATTGACAAAGATGAGATATGCTTATCCATGGTGGAAAGAGAAAGAAATTAACTCCAGAAAGAAGAGGAAATCTGGGAAATGCCCATTAACTCCTGAGGAGACTGCTCTTGCACTGAGGGCATTGGACATTGATCCTAGTATGCAAATATACATAGCTGCTGGTGATATCTATGGGGGAGAAAAAAGAATGGCATCCCTGAGATTAGCCTTTCCACACATAGTTAAGAAGGAAACATTGCTGGAAGCCTCAGAGCTTGAGCCCTTCCGAAACCATTCAAACCAAATGGCTGCACTGGATTACCTAGTATCACTGGAAAGTGATATCTTTGCTCCGACTTATGGAGGAAACATGGCAAGAGTTGTCGAAGGCCATAGACGGTATTTGGGATTCAGGACAACAATTCGTCTGGACAGAATGCTCCTAGTGAATTTGATAGACCAGTACAAGAAGGGAAGACTGAGCTGGGATGAATTTTCCCAGGCAGTTAAAACAGGACATGCCGATCGCATGGGTACCCCAACTCGAAGGCTGGAGATTCCTGGTAAACCCAAGGAAGAGGATTACTTCTATAACAATCCACAGGAATGTTTGCCACCAATTTCTACAAAACCAAAGAGTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

504

Amino Acids

58.27

Weight (kDa)

8.96

Isoelectric Point (pI)

48.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 104 - 424 5.3e-103 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 471
Acc65I GGTACC 1 cut(s) 1400
AccB1I GGYRCC 1 cut(s) 1400
AccIII TCCGGA 1 cut(s) 504
AclWI GGATC 3 cut(s) 679, 692, 1013
AcsI RAATTY 2 cut(s) 1315, 1358
AcuI CTGAAG 1 cut(s) 879
AcyI GRCGYC 1 cut(s) 468
AfaI GTAC 4 cut(s) 146, 856, 1331, 1402
AfiI CCNNNNNNNGG 5 cut(s) 432, 681, 694, 754, 1226
AgsI TTSAA 6 cut(s) 197, 287, 340, 514, 884, 1164
AjnI CCWGG 2 cut(s) 1365, 1426
AluBI AGCT 4 cut(s) 353, 1043, 1141, 1350
AluI AGCT 4 cut(s) 353, 1043, 1141, 1350
Alw26I GTCTC 2 cut(s) 325, 980
AlwI GGATC 3 cut(s) 679, 692, 1013
Aor13HI TCCGGA 1 cut(s) 504
AoxI GGCC 3 cut(s) 17, 797, 1255
ApeKI GCWGC 4 cut(s) 711, 864, 1043, 1175
ApoI RAATTY 2 cut(s) 1315, 1358
Asp700I GAANNNNTTC 1 cut(s) 1159
Asp718I GGTACC 1 cut(s) 1400
AspLEI GCGC 1 cut(s) 711
AspS9I GGNCC 1 cut(s) 797
AsuHPI GGTGA 3 cut(s) 230, 264, 1061
AsuNHI GCTAGC 1 cut(s) 838
AxyI CCTNAGG 2 cut(s) 734, 981
BamHI GGATCC 1 cut(s) 684
BanI GGYRCC 1 cut(s) 1400
BanII GRGCYC 1 cut(s) 1149
BbsI GAAGAC 1 cut(s) 1348
BbvI GCAGC 4 cut(s) 698, 851, 1030, 1162
BccI CCATC 4 cut(s) 283, 310, 544, 822
BciT130I CCWGG 2 cut(s) 1367, 1428
BclI TGATCA 2 cut(s) 229, 439
BcoDI GTCTC 2 cut(s) 325, 980
BfaI CTAG 4 cut(s) 839, 1023, 1190, 1310
BfmI CTRYAG 2 cut(s) 21, 690
BglII AGATCT 1 cut(s) 25
BisI GCNGC 4 cut(s) 712, 865, 1044, 1176
BlsI GCNGC 4 cut(s) 713, 866, 1045, 1177
Bme1390I CCNGG 2 cut(s) 1367, 1428
BmgT120I GGNCC 1 cut(s) 797
BmiI GGNNCC 4 cut(s) 41, 686, 798, 1402
BmrFI CCNGG 2 cut(s) 1367, 1428
BmrI ACTGGG 1 cut(s) 770
BmsI GCATC 2 cut(s) 494, 1088
BmtI GCTAGC 1 cut(s) 842
BmuI ACTGGG 1 cut(s) 770
BpiI GAAGAC 1 cut(s) 1348
BplI GAGNNNNNCTC 2 cut(s) 727, 759
BpmI CTGGAG 2 cut(s) 925, 1439
BpuEI CTTGAG 1 cut(s) 1163
BsaAI YACGTR 1 cut(s) 298
BsaBI GATNNNNATC 1 cut(s) 234
BsaHI GRCGYC 1 cut(s) 468
BsaI GGTCTC 1 cut(s) 325
BsaJI CCNNGG 4 cut(s) 620, 914, 1365, 1437
BsaWI WCCGGW 1 cut(s) 504
Bsc4I CCNNNNNNNGG 5 cut(s) 432, 681, 694, 754, 1226
Bse1I ACTGG 4 cut(s) 765, 1185, 1203, 1327
Bse21I CCTNAGG 2 cut(s) 734, 981
Bse3DI GCAATG 3 cut(s) 17, 502, 1121
Bse8I GATNNNNATC 1 cut(s) 234
BseAI TCCGGA 1 cut(s) 504
BseBI CCWGG 2 cut(s) 1367, 1428
BseDI CCNNGG 4 cut(s) 620, 914, 1365, 1437
BseGI GGATG 3 cut(s) 323, 1079, 1360
BseJI GATNNNNATC 1 cut(s) 234
BseLI CCNNNNNNNGG 5 cut(s) 432, 681, 694, 754, 1226
BseMI GCAATG 3 cut(s) 17, 502, 1121
BseMII CTCAG 8 cut(s) 402, 725, 832, 972, 992, 1076, 1149, 1337
BseNI ACTGG 4 cut(s) 765, 1185, 1203, 1327
BseRI GAGGAG 1 cut(s) 998
BseXI GCAGC 4 cut(s) 698, 851, 1030, 1162
BseYI CCCAGC 2 cut(s) 353, 1350
BsgI GTGCAG 1 cut(s) 1161
Bsh1285I CGRYCG 1 cut(s) 1393
BshFI GGCC 3 cut(s) 19, 799, 1257
BshNI GGYRCC 1 cut(s) 1400
BsiEI CGRYCG 1 cut(s) 1393
BsiSI CCGG 1 cut(s) 505
BslFI GGGAC 1 cut(s) 305
BslI CCNNNNNNNGG 5 cut(s) 432, 681, 694, 754, 1226
BsmAI GTCTC 2 cut(s) 325, 980
BsmFI GGGAC 1 cut(s) 305
BsmI GAATGC 1 cut(s) 1308
BsnI GGCC 3 cut(s) 19, 799, 1257
Bso31I GGTCTC 1 cut(s) 325
Bsp1286I GDGCHC 1 cut(s) 1149
Bsp13I TCCGGA 1 cut(s) 504
Bsp143I GATC 7 cut(s) 25, 229, 439, 472, 684, 1018, 1390
Bsp19I CCATGG 1 cut(s) 914
BspANI GGCC 3 cut(s) 19, 799, 1257
BspCNI CTCAG 8 cut(s) 403, 726, 831, 973, 993, 1077, 1148, 1338
BspEI TCCGGA 1 cut(s) 504
BspLI GGNNCC 4 cut(s) 41, 686, 798, 1402
BspOI GCTAGC 1 cut(s) 842
BspPI GGATC 3 cut(s) 679, 692, 1013
BspT107I GGYRCC 1 cut(s) 1400
BspTNI GGTCTC 1 cut(s) 325
BsrDI GCAATG 3 cut(s) 17, 502, 1121
BsrI ACTGG 4 cut(s) 765, 1185, 1203, 1327
BssECI CCNNGG 4 cut(s) 620, 914, 1365, 1437
BssMI GATC 7 cut(s) 25, 229, 439, 472, 684, 1018, 1390
BssNI GRCGYC 1 cut(s) 468
BssT1I CCWWGG 2 cut(s) 914, 1437
Bst2UI CCWGG 2 cut(s) 1367, 1428
Bst4CI ACNGT 1 cut(s) 1266
Bst6I CTCTTC 5 cut(s) 177, 182, 268, 944, 1437
BstACI GRCGYC 1 cut(s) 468
BstBAI YACGTR 1 cut(s) 298
BstC8I GCNNGC 3 cut(s) 108, 351, 840
BstDEI CTNAG 9 cut(s) 411, 734, 805, 818, 981, 1001, 1085, 1135, 1346
BstDSI CCRYGG 1 cut(s) 914
BstF5I GGATG 3 cut(s) 323, 1079, 1360
BstHHI GCGC 1 cut(s) 711
BstKTI GATC 7 cut(s) 28, 232, 442, 475, 687, 1021, 1393
BstMAI GTCTC 2 cut(s) 325, 980
BstMBI GATC 7 cut(s) 25, 229, 439, 472, 684, 1018, 1390
BstMCI CGRYCG 1 cut(s) 1393
BstMWI GCNNNNNNNGC 2 cut(s) 16, 1004
BstNI CCWGG 2 cut(s) 1367, 1428
BstNSI RCATGY 3 cut(s) 136, 838, 1388
BstSCI CCNGG 2 cut(s) 1365, 1426
BstSFI CTRYAG 2 cut(s) 21, 690
BstV1I GCAGC 4 cut(s) 698, 851, 1030, 1162
BstV2I GAAGAC 1 cut(s) 1348
BstX2I RGATCY 2 cut(s) 25, 684
BstYI RGATCY 2 cut(s) 25, 684
Bsu36I CCTNAGG 2 cut(s) 734, 981
BsuRI GGCC 3 cut(s) 19, 799, 1257
BtgI CCRYGG 1 cut(s) 914
BtgZI GCGATG 1 cut(s) 882
BtsCI GGATG 3 cut(s) 323, 1079, 1360
BtsIMutI CAGTG 3 cut(s) 998, 1178, 1196
Cac8I GCNNGC 3 cut(s) 108, 351, 840
CfoI GCGC 1 cut(s) 711
Cfr13I GGNCC 1 cut(s) 797
Csp6I GTAC 4 cut(s) 145, 855, 1330, 1401
CviAII CATG 8 cut(s) 133, 367, 568, 835, 915, 1237, 1385, 1396
CviQI GTAC 4 cut(s) 145, 855, 1330, 1401
DdeI CTNAG 9 cut(s) 411, 734, 805, 818, 981, 1001, 1085, 1135, 1346
DpnI GATC 7 cut(s) 27, 231, 441, 474, 686, 1020, 1392
DpnII GATC 7 cut(s) 25, 229, 439, 472, 684, 1018, 1390
DraI TTTAAA 1 cut(s) 390
Eam1104I CTCTTC 5 cut(s) 177, 182, 268, 944, 1437
EarI CTCTTC 5 cut(s) 177, 182, 268, 944, 1437
Eco130I CCWWGG 2 cut(s) 914, 1437
Eco147I AGGCCT 1 cut(s) 19
Eco24I GRGCYC 1 cut(s) 1149
Eco31I GGTCTC 1 cut(s) 325
Eco32I GATATC 2 cut(s) 1054, 1210
Eco57I CTGAAG 1 cut(s) 879
Eco81I CCTNAGG 2 cut(s) 734, 981
EcoRII CCWGG 2 cut(s) 1365, 1426
EcoRV GATATC 2 cut(s) 1054, 1210
EcoT14I CCWWGG 2 cut(s) 914, 1437
EcoT22I ATGCAT 2 cut(s) 6, 134
EcoT38I GRGCYC 1 cut(s) 1149
ErhI CCWWGG 2 cut(s) 914, 1437
FaeI CATG 8 cut(s) 136, 370, 571, 838, 918, 1240, 1388, 1399
FaqI GGGAC 1 cut(s) 305
FatI CATG 8 cut(s) 132, 366, 567, 834, 914, 1236, 1384, 1395
FbaI TGATCA 2 cut(s) 229, 439
Fnu4HI GCNGC 4 cut(s) 712, 865, 1044, 1176
FokI GGATG 3 cut(s) 310, 1066, 1367
FriOI GRGCYC 1 cut(s) 1149
Fsp4HI GCNGC 4 cut(s) 712, 865, 1044, 1176
FspBI CTAG 4 cut(s) 839, 1023, 1190, 1310
GlaI GCGC 1 cut(s) 710
GluI GCNGC 4 cut(s) 712, 865, 1044, 1176
GsaI CCCAGC 2 cut(s) 357, 1354
GsuI CTGGAG 2 cut(s) 925, 1439
HaeIII GGCC 3 cut(s) 19, 799, 1257
HapII CCGG 1 cut(s) 505
HhaI GCGC 1 cut(s) 711
Hin1I GRCGYC 1 cut(s) 468
Hin1II CATG 8 cut(s) 136, 370, 571, 838, 918, 1240, 1388, 1399
Hin6I GCGC 1 cut(s) 709
HinP1I GCGC 1 cut(s) 709
HinfI GANTC 7 cut(s) 67, 340, 445, 613, 1275, 1423, 1504
HpaII CCGG 1 cut(s) 505
HphI GGTGA 3 cut(s) 230, 264, 1061
Hpy166II GTNNAC 3 cut(s) 301, 741, 1433
Hpy188I TCNGA 9 cut(s) 72, 315, 444, 624, 702, 821, 1138, 1154, 1221
Hpy188III TCNNGA 7 cut(s) 160, 410, 505, 942, 980, 1279, 1295
Hpy8I GTNNAC 3 cut(s) 301, 741, 1433
Hpy99I CGWCG 2 cut(s) 470, 473
HpyAV CCTTC 6 cut(s) 854, 1108, 1159, 1247, 1330, 1407
HpyCH4III ACNGT 1 cut(s) 1266
HpyCH4IV ACGT 3 cut(s) 297, 468, 596
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 1004
HpyF3I CTNAG 9 cut(s) 411, 734, 805, 818, 981, 1001, 1085, 1135, 1346
HpySE526I ACGT 3 cut(s) 297, 468, 596
Hsp92I GRCGYC 1 cut(s) 468
Hsp92II CATG 8 cut(s) 136, 370, 571, 838, 918, 1240, 1388, 1399
HspAI GCGC 1 cut(s) 709
Kpn2I TCCGGA 1 cut(s) 504
KpnI GGTACC 1 cut(s) 1404
Ksp22I TGATCA 2 cut(s) 229, 439
Kzo9I GATC 7 cut(s) 25, 229, 439, 472, 684, 1018, 1390
LmnI GCTCC 3 cut(s) 39, 1222, 1311
Lsp1109I GCAGC 4 cut(s) 698, 851, 1030, 1162
LweI GCATC 2 cut(s) 494, 1088
MaeI CTAG 4 cut(s) 839, 1023, 1190, 1310
MaeII ACGT 3 cut(s) 297, 468, 596
MaeIII GTNAC 2 cut(s) 362, 394
MalI GATC 7 cut(s) 27, 231, 441, 474, 686, 1020, 1392
MboI GATC 7 cut(s) 25, 229, 439, 472, 684, 1018, 1390
MfeI CAATTG 1 cut(s) 750
MflI RGATCY 2 cut(s) 25, 684
MhlI GDGCHC 1 cut(s) 1149
MlyI GAGTC 2 cut(s) 76, 454
MmeI TCCRAC 2 cut(s) 396, 1244
Mph1103I ATGCAT 2 cut(s) 6, 134
MroI TCCGGA 1 cut(s) 504
MroXI GAANNNNTTC 1 cut(s) 1159
MseI TTAA 5 cut(s) 389, 936, 974, 1110, 1374
MslI CAYNNNNRTG 1 cut(s) 1471
MspI CCGG 1 cut(s) 505
MspR9I CCNGG 2 cut(s) 1367, 1428
MunI CAATTG 1 cut(s) 750
Mva1269I GAATGC 1 cut(s) 1308
MvaI CCWGG 2 cut(s) 1367, 1428
MwoI GCNNNNNNNGC 2 cut(s) 16, 1004
NcoI CCATGG 1 cut(s) 914
NdeII GATC 7 cut(s) 25, 229, 439, 472, 684, 1018, 1390
NheI GCTAGC 1 cut(s) 838
NlaIII CATG 8 cut(s) 136, 370, 571, 838, 918, 1240, 1388, 1399
NlaIV GGNNCC 4 cut(s) 41, 686, 798, 1402
NmuCI GTSAC 2 cut(s) 362, 394
NsiI ATGCAT 2 cut(s) 6, 134
NspI RCATGY 3 cut(s) 136, 838, 1388
PceI AGGCCT 1 cut(s) 19
PctI GAATGC 1 cut(s) 1308
PdmI GAANNNNTTC 1 cut(s) 1159
PfeI GAWTC 4 cut(s) 340, 613, 1275, 1423
PkrI GCNGC 4 cut(s) 713, 866, 1045, 1177
Ple19I CGATCG 1 cut(s) 1393
PleI GAGTC 2 cut(s) 75, 453
PpsI GAGTC 2 cut(s) 75, 453
Ppu21I YACGTR 1 cut(s) 298
Psp6I CCWGG 2 cut(s) 1365, 1426
PspFI CCCAGC 2 cut(s) 353, 1350
PspGI CCWGG 2 cut(s) 1365, 1426
PspN4I GGNNCC 4 cut(s) 41, 686, 798, 1402
PspPI GGNCC 1 cut(s) 797
PsuI RGATCY 2 cut(s) 25, 684
PvuI CGATCG 1 cut(s) 1393
RsaI GTAC 4 cut(s) 146, 856, 1331, 1402
RsaNI GTAC 4 cut(s) 145, 855, 1330, 1401
RseI CAYNNNNRTG 1 cut(s) 1471
SaqAI TTAA 5 cut(s) 389, 936, 974, 1110, 1374
SatI GCNGC 4 cut(s) 712, 865, 1044, 1176
Sau3AI GATC 7 cut(s) 25, 229, 439, 472, 684, 1018, 1390
Sau96I GGNCC 1 cut(s) 797
SchI GAGTC 2 cut(s) 76, 454
ScrFI CCNGG 2 cut(s) 1367, 1428
SduI GDGCHC 1 cut(s) 1149
SfaNI GCATC 2 cut(s) 494, 1088
SfcI CTRYAG 2 cut(s) 21, 690
SmiMI CAYNNNNRTG 1 cut(s) 1471
SmlI CTYRAG 1 cut(s) 1142
SmoI CTYRAG 1 cut(s) 1142
SseBI AGGCCT 1 cut(s) 19
SspMI CTAG 4 cut(s) 839, 1023, 1190, 1310
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 2 cut(s) 1365, 1426
StyI CCWWGG 2 cut(s) 914, 1437
TaaI ACNGT 1 cut(s) 1266
TaiI ACGT 3 cut(s) 300, 471, 599
TaqI TCGA 5 cut(s) 137, 465, 471, 1251, 1411
TatI WGTACW 2 cut(s) 854, 1329
TfiI GAWTC 4 cut(s) 340, 613, 1275, 1423
Tru1I TTAA 5 cut(s) 389, 936, 974, 1110, 1374
Tru9I TTAA 5 cut(s) 389, 936, 974, 1110, 1374
TscAI CASTG 3 cut(s) 1005, 1185, 1203
TseFI GTSAC 2 cut(s) 362, 394
TseI GCWGC 4 cut(s) 711, 864, 1043, 1175
Tsp45I GTSAC 2 cut(s) 362, 394
TspDTI ATGAA 7 cut(s) 469, 513, 651, 741, 840, 891, 1371
TspRI CASTG 3 cut(s) 1005, 1185, 1203
XapI RAATTY 2 cut(s) 1315, 1358
XceI RCATGY 3 cut(s) 136, 838, 1388
XmnI GAANNNNTTC 1 cut(s) 1159
XspI CTAG 4 cut(s) 839, 1023, 1190, 1310
ZraI GACGTC 1 cut(s) 469
Zsp2I ATGCAT 2 cut(s) 6, 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.