FvH4_4g29250

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004856
Physical Location & Seq
Reverse (-)
29457474 .. 29459995
2522 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g29250.t1

Sequence Viewer

Length: 1545 bp
ATGGATGGAAAAGATGATGATCTGAAGGAGCCAGAATATGGGCAGGAGGTGAAGGAGAAGTCTTACATTAGTGGTAAGCTCAAGTACTGGAAATACGCTGGAGGAATGAAGGCTGAACTGTTGAAGGATTTGATGAACGGGACTAATCTCAAAACCGAGAAGTACTTGAAAGGTTTGGTGATAGCTAGGGCAAGACTCGCCCGGTTGAAATTATGGGCGGTTCGACTCACCAAATTGCTGCTACTTCTGGCTATTGCATTGCAAATCAAGGGTTTGGGTGATACCTTGATATCCAGCTTCTCTCTTCCCCATGAAAGGATTTACAAAAACAATGGATATTTGATGGTTTCATCCAACGGAGGACTAAATCAGATGCGATCTGGTATATGTGACATGGTAGCAATTGCAAGATACTTAAACGTCACGCTTATAGTTCCTGAGTTGGATAACACCTCCTTCTGGAATGACCGCAGTCAATTTGAAGACATATTCGATGTGGATTATTTCATTACCTCGTTGAGAGATGAGGTCAAAATTTTGAGAGAACTACCTCCTAAGCAGAAGAAGAAAGTGGAATTAGAGTCCCTTTATTCCATGCCCCCGATTAGTTGGTCCAACATGACTTACTACTATAACACGATTCTTCCTCGCATACAAACATACGAAGTTGTACACTTCACAAAAACAGATGCTAGGCTTGCCAACAATGGCATCCCTGAAGAGGTTCAGAAGCTTCGCTGCCGTGCTAACTACCAGGCTCTGAGATTCTCTCCTGTGATTGAGGCATTAGGCAAGAAAATCGTTAATATCCTCCGCGAAAGAGGCCCTTTCTTGGTTCTTCATCTCCGGTACGAAATGGACATGGTGGCCTTTTCTGGTTGCACTGAGGGTTGCACTCGAGAAGAGATTGAAGAGACCACGAAAATGAGATATGCTTATCCATGGTGGAAAGAAAAGGAGATTGATTCAGAGAAGAAGAGGAAGGATGGACTTTGCCCTTTAACACCTGAAGAAACTACTCTAGCATTGAGAGCACTAGGCATTGATCGTAATATCCAAGTCTATATTGCTGCTGGAGATATATACAAGGCAGAGAGGAGACTGGCACCTCTCAAAGAAGCTTTTCCAAATTTGGTGAGAAAGGAGACACTAGTTGATGCATCAGATCTTCTTCCTCTCCAAAACCACTCGAACCAGATGGCGGCTCTGGACTACTACGTCTCGATAGAAAGTGACATCTTTGTCCCAACATATGGAGGAAACATGGCAAAAGTAGTTGAAGGCCATAGAAGATACTTGGGATTCAAGAAAACCATTCTTCTAGACAGGAAGGCTCTGGTGGATCTGATAGACCAGTACAACAATGGAACCCTGAGCTGGAACGAGTTCGCCGTCGGAGTCAATACTGTTCATGCAGACCGTATGGGAAACCCAACTTGGAGATTGGAGGTTCCAGGAAAACCTAAACTAGAGGACTACTTCTATACAAACCCAACTGAGTGTTTAACAATGCCGGCTGAAGATGATGATCAACTATTACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

515

Amino Acids

59.11

Weight (kDa)

7.54

Isoelectric Point (pI)

37.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 113 - 433 1.7e-102 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1217, 1241
AccB1I GGYRCC 1 cut(s) 1105
AccB7I CCANNNNNTGG 2 cut(s) 38, 1253
AccII CGCG 1 cut(s) 816
AciI CCGC 4 cut(s) 218, 469, 814, 1202
AclWI GGATC 1 cut(s) 1350
AcsI RAATTY 2 cut(s) 534, 1129
AcuI CTGAAG 4 cut(s) 44, 738, 1029, 1539
AfaI GTAC 5 cut(s) 86, 164, 672, 851, 1358
AfiI CCNNNNNNNGG 8 cut(s) 38, 315, 459, 721, 832, 1201, 1253, 1377
AgsI TTSAA 7 cut(s) 124, 169, 208, 482, 911, 1280, 1306
AhlI ACTAGT 1 cut(s) 1150
AjnI CCWGG 2 cut(s) 753, 1453
AjuI GAANNNNNNNTTGG 2 cut(s) 1420, 1452
AluBI AGCT 6 cut(s) 79, 185, 297, 733, 1121, 1377
AluI AGCT 6 cut(s) 79, 185, 297, 733, 1121, 1377
Alw21I GWGCWC 1 cut(s) 1036
Alw26I GTCTC 4 cut(s) 908, 1093, 1139, 1225
AlwI GGATC 1 cut(s) 1350
AlwNI CAGNNNCTG 1 cut(s) 760
Ama87I CYCGRG 1 cut(s) 897
AoxI GGCC 3 cut(s) 823, 867, 1282
ApeKI GCWGC 3 cut(s) 238, 738, 1070
ApoI RAATTY 2 cut(s) 534, 1129
Asp700I GAANNNNTTC 3 cut(s) 723, 1122, 1385
AspS9I GGNCC 2 cut(s) 612, 824
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 5 cut(s) 61, 190, 220, 290, 1147
AvaI CYCGRG 1 cut(s) 897
AvaII GGWCC 1 cut(s) 612
BanI GGYRCC 1 cut(s) 1105
BbsI GAAGAC 1 cut(s) 489
Bbv12I GWGCWC 1 cut(s) 1036
BbvI GCAGC 3 cut(s) 225, 725, 1057
BccI CCATC 3 cut(s) 337, 980, 1192
BceAI ACGGC 2 cut(s) 726, 1376
BcgI CGANNNNNNTGC 2 cut(s) 781, 815
BciT130I CCWGG 2 cut(s) 755, 1455
BclI TGATCA 1 cut(s) 1528
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 4 cut(s) 908, 1093, 1139, 1225
BcuI ACTAGT 1 cut(s) 1150
BfaI CTAG 7 cut(s) 186, 693, 1022, 1037, 1151, 1322, 1469
BglII AGATCT 1 cut(s) 1165
BisI GCNGC 4 cut(s) 239, 739, 1071, 1203
BlsI GCNGC 4 cut(s) 240, 740, 1072, 1204
BmcAI AGTACT 2 cut(s) 86, 164
Bme1390I CCNGG 3 cut(s) 202, 755, 1455
Bme18I GGWCC 1 cut(s) 612
BmeT110I CYCGRG 1 cut(s) 897
BmgT120I GGNCC 2 cut(s) 612, 824
BmiI GGNNCC 4 cut(s) 30, 1107, 1369, 1452
BmrFI CCNGG 3 cut(s) 202, 755, 1455
BmsI GCATC 5 cut(s) 363, 679, 720, 1147, 1169
BoxI GACNNNNGTC 1 cut(s) 471
BpiI GAAGAC 1 cut(s) 489
BplI GAGNNNNNCTC 2 cut(s) 754, 786
BpmI CTGGAG 2 cut(s) 120, 1095
Bpu10I CCTNAGC 2 cut(s) 555, 1373
BpuEI CTTGAG 1 cut(s) 65
BpuMI CCSGG 1 cut(s) 202
BsaBI GATNNNNATC 2 cut(s) 18, 1527
BsaI GGTCTC 1 cut(s) 908
BsaJI CCNNGG 1 cut(s) 941
BsaWI WCCGGW 1 cut(s) 846
Bsc4I CCNNNNNNNGG 8 cut(s) 38, 315, 459, 721, 832, 1201, 1253, 1377
Bse118I RCCGGY 1 cut(s) 1513
Bse1I ACTGG 3 cut(s) 92, 1107, 1354
Bse3DI GCAATG 1 cut(s) 257
Bse8I GATNNNNATC 2 cut(s) 18, 1527
BseBI CCWGG 2 cut(s) 755, 1455
BseDI CCNNGG 1 cut(s) 941
BseGI GGATG 4 cut(s) 10, 350, 711, 991
BseJI GATNNNNATC 2 cut(s) 18, 1527
BseLI CCNNNNNNNGG 8 cut(s) 38, 315, 459, 721, 832, 1201, 1253, 1377
BseMI GCAATG 1 cut(s) 257
BseMII CTCAG 5 cut(s) 429, 752, 876, 1364, 1488
BseNI ACTGG 3 cut(s) 92, 1107, 1354
BseRI GAGGAG 1 cut(s) 1111
BseXI GCAGC 3 cut(s) 225, 725, 1057
Bsh1236I CGCG 1 cut(s) 816
BshFI GGCC 3 cut(s) 825, 869, 1284
BshNI GGYRCC 1 cut(s) 1105
BsiHKAI GWGCWC 1 cut(s) 1036
BsiHKCI CYCGRG 1 cut(s) 897
BsiSI CCGG 3 cut(s) 202, 847, 1514
BslFI GGGAC 3 cut(s) 154, 568, 1229
BslI CCNNNNNNNGG 8 cut(s) 38, 315, 459, 721, 832, 1201, 1253, 1377
BsmAI GTCTC 4 cut(s) 908, 1093, 1139, 1225
BsmBI CGTCTC 1 cut(s) 1225
BsmFI GGGAC 3 cut(s) 154, 568, 1229
BsnI GGCC 3 cut(s) 825, 869, 1284
Bso31I GGTCTC 1 cut(s) 908
BsoBI CYCGRG 1 cut(s) 897
Bsp1286I GDGCHC 1 cut(s) 1036
Bsp1407I TGTACA 1 cut(s) 670
Bsp143I GATC 6 cut(s) 19, 377, 1045, 1165, 1342, 1528
Bsp19I CCATGG 1 cut(s) 941
BspACI CCGC 4 cut(s) 218, 469, 814, 1202
BspANI GGCC 3 cut(s) 825, 869, 1284
BspCNI CTCAG 5 cut(s) 430, 753, 877, 1365, 1489
BspFNI CGCG 1 cut(s) 816
BspLI GGNNCC 4 cut(s) 30, 1107, 1369, 1452
BspPI GGATC 1 cut(s) 1350
BspT107I GGYRCC 1 cut(s) 1105
BspTNI GGTCTC 1 cut(s) 908
BsrDI GCAATG 1 cut(s) 257
BsrFI RCCGGY 1 cut(s) 1513
BsrGI TGTACA 1 cut(s) 670
BsrI ACTGG 3 cut(s) 92, 1107, 1354
BssAI RCCGGY 1 cut(s) 1513
BssECI CCNNGG 1 cut(s) 941
BssMI GATC 6 cut(s) 19, 377, 1045, 1165, 1342, 1528
BssT1I CCWWGG 1 cut(s) 941
Bst2UI CCWGG 2 cut(s) 755, 1455
Bst4CI ACNGT 4 cut(s) 120, 1408, 1421, 1542
Bst6I CTCTTC 5 cut(s) 309, 714, 897, 906, 971
BstAUI TGTACA 1 cut(s) 670
BstC8I GCNNGC 2 cut(s) 699, 1515
BstDEI CTNAG 6 cut(s) 438, 555, 761, 885, 1373, 1497
BstDSI CCRYGG 1 cut(s) 941
BstF5I GGATG 4 cut(s) 10, 350, 711, 991
BstFNI CGCG 1 cut(s) 816
BstKTI GATC 6 cut(s) 22, 380, 1048, 1168, 1345, 1531
BstMAI GTCTC 4 cut(s) 908, 1093, 1139, 1225
BstMBI GATC 6 cut(s) 19, 377, 1045, 1165, 1342, 1528
BstMWI GCNNNNNNNGC 4 cut(s) 197, 698, 822, 1031
BstNI CCWGG 2 cut(s) 755, 1455
BstPAI GACNNNNGTC 1 cut(s) 471
BstSCI CCNGG 3 cut(s) 200, 753, 1453
BstUI CGCG 1 cut(s) 816
BstV1I GCAGC 3 cut(s) 225, 725, 1057
BstV2I GAAGAC 1 cut(s) 489
BstX2I RGATCY 2 cut(s) 1165, 1342
BstYI RGATCY 2 cut(s) 1165, 1342
BsuRI GGCC 3 cut(s) 825, 869, 1284
BtgI CCRYGG 1 cut(s) 941
BtsCI GGATG 4 cut(s) 10, 350, 711, 991
BtsIMutI CAGTG 1 cut(s) 882
Cac8I GCNNGC 2 cut(s) 699, 1515
CaiI CAGNNNCTG 1 cut(s) 760
Cfr10I RCCGGY 1 cut(s) 1513
Cfr13I GGNCC 2 cut(s) 612, 824
Csp6I GTAC 5 cut(s) 85, 163, 671, 850, 1357
CviAII CATG 8 cut(s) 311, 394, 595, 619, 862, 942, 1264, 1412
CviQI GTAC 5 cut(s) 85, 163, 671, 850, 1357
DdeI CTNAG 6 cut(s) 438, 555, 761, 885, 1373, 1497
DpnI GATC 6 cut(s) 21, 379, 1047, 1167, 1344, 1530
DpnII GATC 6 cut(s) 19, 377, 1045, 1165, 1342, 1528
DrdI GACNNNNNNGTC 2 cut(s) 1217, 1241
DseDI GACNNNNNNGTC 2 cut(s) 1217, 1241
Eam1104I CTCTTC 5 cut(s) 309, 714, 897, 906, 971
EarI CTCTTC 5 cut(s) 309, 714, 897, 906, 971
Eco130I CCWWGG 1 cut(s) 941
Eco31I GGTCTC 1 cut(s) 908
Eco32I GATATC 1 cut(s) 291
Eco47I GGWCC 1 cut(s) 612
Eco57I CTGAAG 4 cut(s) 44, 738, 1029, 1539
Eco88I CYCGRG 1 cut(s) 897
EcoO109I RGGNCCY 1 cut(s) 824
EcoRII CCWGG 2 cut(s) 753, 1453
EcoRV GATATC 1 cut(s) 291
EcoT14I CCWWGG 1 cut(s) 941
EcoT22I ATGCAT 1 cut(s) 1162
ErhI CCWWGG 1 cut(s) 941
Esp3I CGTCTC 1 cut(s) 1225
FaeI CATG 8 cut(s) 314, 397, 598, 622, 865, 945, 1267, 1415
FalI AAGNNNNNCTT 2 cut(s) 811, 843
FaqI GGGAC 3 cut(s) 154, 568, 1229
FatI CATG 8 cut(s) 310, 393, 594, 618, 861, 941, 1263, 1411
FauNDI CATATG 1 cut(s) 1252
FbaI TGATCA 1 cut(s) 1528
Fnu4HI GCNGC 4 cut(s) 239, 739, 1071, 1203
FokI GGATG 4 cut(s) 17, 337, 698, 998
Fsp4HI GCNGC 4 cut(s) 239, 739, 1071, 1203
FspBI CTAG 7 cut(s) 186, 693, 1022, 1037, 1151, 1322, 1469
GluI GCNGC 4 cut(s) 239, 739, 1071, 1203
GsuI CTGGAG 2 cut(s) 120, 1095
HaeIII GGCC 3 cut(s) 825, 869, 1284
HapII CCGG 3 cut(s) 202, 847, 1514
Hin1II CATG 8 cut(s) 314, 397, 598, 622, 865, 945, 1267, 1415
HindIII AAGCTT 2 cut(s) 731, 1119
HinfI GANTC 8 cut(s) 195, 225, 581, 640, 765, 965, 1302, 1398
HpaII CCGG 3 cut(s) 202, 847, 1514
HphI GGTGA 5 cut(s) 61, 190, 220, 290, 1147
Hpy166II GTNNAC 1 cut(s) 673
Hpy188I TCNGA 8 cut(s) 24, 372, 729, 762, 970, 1165, 1347, 1397
Hpy188III TCNNGA 7 cut(s) 437, 460, 899, 1208, 1222, 1306, 1322
Hpy8I GTNNAC 1 cut(s) 673
Hpy99I CGWCG 1 cut(s) 1397
HpyAV CCTTC 8 cut(s) 19, 46, 103, 118, 466, 976, 1274, 1324
HpyCH4III ACNGT 4 cut(s) 120, 1408, 1421, 1542
HpyCH4IV ACGT 2 cut(s) 420, 1218
HpyCH4V TGCA 7 cut(s) 257, 262, 407, 882, 894, 1160, 1415
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 698, 822, 1031
HpyF3I CTNAG 6 cut(s) 438, 555, 761, 885, 1373, 1497
HpySE526I ACGT 2 cut(s) 420, 1218
Hsp92II CATG 8 cut(s) 314, 397, 598, 622, 865, 945, 1267, 1415
KroI GCCGGC 1 cut(s) 1513
KroNI GCCGGC 1 cut(s) 1515
Ksp22I TGATCA 1 cut(s) 1528
Kzo9I GATC 6 cut(s) 19, 377, 1045, 1165, 1342, 1528
LmnI GCTCC 1 cut(s) 28
Lsp1109I GCAGC 3 cut(s) 225, 725, 1057
LweI GCATC 5 cut(s) 363, 679, 720, 1147, 1169
MaeI CTAG 7 cut(s) 186, 693, 1022, 1037, 1151, 1322, 1469
MaeII ACGT 2 cut(s) 420, 1218
MaeIII GTNAC 3 cut(s) 389, 421, 1232
MalI GATC 6 cut(s) 21, 379, 1047, 1167, 1344, 1530
MboI GATC 6 cut(s) 19, 377, 1045, 1165, 1342, 1528
MfeI CAATTG 1 cut(s) 402
MflI RGATCY 2 cut(s) 1165, 1342
MhlI GDGCHC 1 cut(s) 1036
MluCI AATT 7 cut(s) 209, 233, 402, 476, 534, 575, 1129
MlyI GAGTC 4 cut(s) 189, 219, 590, 1407
MmeI TCCRAC 4 cut(s) 378, 423, 639, 1375
Mph1103I ATGCAT 1 cut(s) 1162
MroNI GCCGGC 1 cut(s) 1513
MroXI GAANNNNTTC 3 cut(s) 723, 1122, 1385
MseI TTAA 4 cut(s) 416, 804, 1001, 1505
MslI CAYNNNNRTG 1 cut(s) 923
MspI CCGG 3 cut(s) 202, 847, 1514
MspR9I CCNGG 3 cut(s) 202, 755, 1455
MunI CAATTG 1 cut(s) 402
MvaI CCWGG 2 cut(s) 755, 1455
MvnI CGCG 1 cut(s) 816
MwoI GCNNNNNNNGC 4 cut(s) 197, 698, 822, 1031
NaeI GCCGGC 1 cut(s) 1515
NciI CCSGG 1 cut(s) 202
NcoI CCATGG 1 cut(s) 941
NdeI CATATG 1 cut(s) 1252
NdeII GATC 6 cut(s) 19, 377, 1045, 1165, 1342, 1528
NgoMIV GCCGGC 1 cut(s) 1513
NlaIII CATG 8 cut(s) 314, 397, 598, 622, 865, 945, 1267, 1415
NlaIV GGNNCC 4 cut(s) 30, 1107, 1369, 1452
NmuCI GTSAC 3 cut(s) 389, 421, 1232
NsiI ATGCAT 1 cut(s) 1162
PaeR7I CTCGAG 1 cut(s) 897
PcsI WCGNNNNNNNCGW 1 cut(s) 1389
PdiI GCCGGC 1 cut(s) 1515
PdmI GAANNNNTTC 3 cut(s) 723, 1122, 1385
PfeI GAWTC 4 cut(s) 640, 765, 965, 1302
PflMI CCANNNNNTGG 2 cut(s) 38, 1253
PfoI TCCNGGA 1 cut(s) 1453
PkrI GCNGC 4 cut(s) 240, 740, 1072, 1204
PleI GAGTC 4 cut(s) 189, 219, 589, 1406
PpsI GAGTC 4 cut(s) 189, 219, 589, 1406
PshAI GACNNNNGTC 1 cut(s) 471
Psp6I CCWGG 2 cut(s) 753, 1453
PspGI CCWGG 2 cut(s) 753, 1453
PspN4I GGNNCC 4 cut(s) 30, 1107, 1369, 1452
PspPI GGNCC 2 cut(s) 612, 824
PstNI CAGNNNCTG 1 cut(s) 760
PsuI RGATCY 2 cut(s) 1165, 1342
RsaI GTAC 5 cut(s) 86, 164, 672, 851, 1358
RsaNI GTAC 5 cut(s) 85, 163, 671, 850, 1357
RseI CAYNNNNRTG 1 cut(s) 923
SaqAI TTAA 4 cut(s) 416, 804, 1001, 1505
SatI GCNGC 4 cut(s) 239, 739, 1071, 1203
Sau3AI GATC 6 cut(s) 19, 377, 1045, 1165, 1342, 1528
Sau96I GGNCC 2 cut(s) 612, 824
ScaI AGTACT 2 cut(s) 86, 164
SchI GAGTC 4 cut(s) 189, 219, 590, 1407
ScrFI CCNGG 3 cut(s) 202, 755, 1455
SduI GDGCHC 1 cut(s) 1036
SfaNI GCATC 5 cut(s) 363, 679, 720, 1147, 1169
Sfr274I CTCGAG 1 cut(s) 897
SinI GGWCC 1 cut(s) 612
SlaI CTCGAG 1 cut(s) 897
SmiMI CAYNNNNRTG 1 cut(s) 923
SmlI CTYRAG 2 cut(s) 80, 897
SmoI CTYRAG 2 cut(s) 80, 897
SpeI ACTAGT 1 cut(s) 1150
Sse9I AATT 7 cut(s) 209, 233, 402, 476, 534, 575, 1129
SsiI CCGC 4 cut(s) 218, 469, 814, 1202
SspMI CTAG 7 cut(s) 186, 693, 1022, 1037, 1151, 1322, 1469
StyD4I CCNGG 3 cut(s) 200, 753, 1453
StyI CCWWGG 1 cut(s) 941
TaaI ACNGT 4 cut(s) 120, 1408, 1421, 1542
TaiI ACGT 2 cut(s) 423, 1221
TaqI TCGA 5 cut(s) 223, 492, 898, 1190, 1223
TasI AATT 7 cut(s) 209, 233, 402, 476, 534, 575, 1129
TatI WGTACW 4 cut(s) 84, 162, 670, 1356
TauI GCSGC 1 cut(s) 1205
TfiI GAWTC 4 cut(s) 640, 765, 965, 1302
Tru1I TTAA 4 cut(s) 416, 804, 1001, 1505
Tru9I TTAA 4 cut(s) 416, 804, 1001, 1505
TscAI CASTG 1 cut(s) 889
TseFI GTSAC 3 cut(s) 389, 421, 1232
TseI GCWGC 3 cut(s) 238, 738, 1070
Tsp45I GTSAC 3 cut(s) 389, 421, 1232
TspDTI ATGAA 7 cut(s) 122, 149, 327, 339, 496, 830, 1400
TspGWI ACGGA 1 cut(s) 372
TspRI CASTG 1 cut(s) 889
Van91I CCANNNNNTGG 2 cut(s) 38, 1253
VpaK11BI GGWCC 1 cut(s) 612
XapI RAATTY 2 cut(s) 534, 1129
XbaI TCTAGA 1 cut(s) 1321
XcmI CCANNNNNNNNNTGG 1 cut(s) 1361
XhoI CTCGAG 1 cut(s) 897
XmnI GAANNNNTTC 3 cut(s) 723, 1122, 1385
XspI CTAG 7 cut(s) 186, 693, 1022, 1037, 1151, 1322, 1469
ZrmI AGTACT 2 cut(s) 86, 164
Zsp2I ATGCAT 1 cut(s) 1162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.